Chy4G086120 (gene) Cucumber (hystrix) v1

Overview
NameChy4G086120
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationchrH04: 25014435 .. 25018446 (-)
RNA-Seq ExpressionChy4G086120
SyntenyChy4G086120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCCCAGCTAAAACCGGCTCGATTATAACAACAGGGAACGTGGCATCAAATATCGGCGGTGCGGCGTCACACGCTTACACATGCTCGAAGCATGCGGTAGTGGGGTTGATGAAGAACGCGGCGGGAGAGTTGGGGCAGTTCGGGATTAGGTGAATTGTTTATCTCCGTATGGGTTGATGACGGGGATGGCGAGGAAGTTGGATGAGATGCAGTTTGAGATGATTATGAGTTTCAGGGCAAATTTGAAAGGAGTGACGCTTAAGGTTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGTGATGAAAGTAGGTATGTGAGTGGCCATAATTTGTTGATTGATGGAGGTATTAGTATTGTTAATCCTAATATCCAAAATTTTCAATACCCTCAAAATTAGGTGCTTGTTGGTCCGAAGGTAAAATTATTAGAAGAAGCTATGAGTAGTTTAGTTATTAGAAAAGATAGATTTCTAAATCTTTAACCAATGGTTCAATATTATTGTGTTCTTCGCTTAATTGCTTTTTTTGTACACTATGAACTTTTGTGTAATAAATGAAGCTGTATCAAAGAAATAACTATAACGATAGATTATTTTGAGTAAAACTTGGATATGGGCAGGTGTCACCCAGCACTTGCATCCCTCTTCCTCAGTTTTCTTTAATTTCCTTCTTCATCCAAATCTCAATTTCACTTCCTTTTTCTTCCTTCATCAATCTCCTAATCGTTCTTTGTTCTTATTTTTAAAGATTGGATTTATATTATATATTAAAATGAAAGAGCAGTGGACATGAGTTATGAGTTTAATTTTAAATATTGAGAAAGCTGTTGTAATAGTTTTCTACGTCTTTTAATGATAATTAATAAATTAGTGTTATATATAATTTTAATATACCAACATTAACATAATTCAATTATCTTGTAATATTGAGCTTAAACTAAGATCCTCAATTCCTTAAAAAGTAATATTTATACTACTCATCTAAGGTAAGAACACTAAAATCTCGCAAAGGTTTATTAATATATTAATATGCTAGTATAAACTCATCCAAAGTGTTATATAAATATACTTTTCTTCAAATTTAAAGTACACGTAAATGTACTAACATTATTATTTTCTTTTTGGCCTGTTTTTTGGATGGTGAAAGGAGGACTGAGTGTGAATTATTCAATTTGAAAATGGCTACTTATTAAATGACTTTTTCATCTTCTTTGTATTTTCTTCTTCTTTTTCTTCCTAATCGACATATTGTATGAAACTTATGATTTGAGTTAATTAATTGCATAGATTAAAGTAAGTGCACGAACAATAATTATTATTGTCCTTGAAAACAACAAAGCATATTGAAAATGAATGGTGGGGTAAATGCAGGTAGATTTCTTAAAGCTTTCATTAAGAGTTTAAATTCATTCCTCTGTAAAAGTTTAAGACTTTAATAATTTATATTTTTTTTAAAAAAGATATATAAATTAGGAGGTGGACTAGAACATCTTCACTTGATGGACATATTCATCATCTTCCATCTGATATGTGTTCAATAAACACGGAGCGAGATATAATAGTACAAAAGTTTGACACAATTTTTTTTTCTAAAAGGAACATAATGGTGAGTATATATTGATACAATTATCAAAGTTTTGAGTTCAAGTTGATACATCACTCACACTTTAGGGGTTTAAATTTATTTAAAAAAAAAAAAAAAACTAAAATGGCCAATAAAAGAAGGGACCAGTAGTGAAGTGAGAAAAAAAAAATGGAGAATGAGTGAAACTTGCAACTTTCGACTTTTGAGACATTCAATGAAACATTTAATCATACCTAATTGCATTTAAACTTTTATCTATTTTGAATATAAATTCATTCACACAAAATTTGAAGAGTGCTTTTGCGAAAATTTAAAAAAAATTATGATAATAGGGTCTATGTCTCTATATTTTCTAAATTAATTAAATAGCAAATTTAAAAGTCAATAACTCTTTGATAGTTTTCTAATAGTCGTCCAATACCTACGTAAATCTGTCGGTGCTTAGATGCATTATTAAAAAATTGGTCATTGTCTTTCCAGCCATACACACCATAACAAAGCTCACAATATATTTCTTCTCAGCCATTGGTTTATAGGACCACAATATTTATATATTTATATATATATATATATATATATATTTTAATTATAATTGTTTAAGCATAATTAATCATATATGAATTATACCTTCCTAAAATTGGGAAGTTATGTTGTTTTTTTATTCGTCTAAGCCATCTAAAGATAAAATTCTCAAATTATATTATACTTTAATCGACTAAATTGAAAATTAATTTGAAAGTGGTTTAATTTTCTCAAATTTTCTTTTTTACGAATTTAAACCTATGAAACCCCACATAACTTTAGGGGGAAATGTTATATAATTATAGGACGACTATGTATATCTAGGTTTTATTATTATTATTATTATTATGAAAGATAATTATTGGTTTGTGGATTAAAAATTTTGATACGGCTTTGGATACAAAATTTTTAATTATTTTTTTTAAAAAAATCATCAGCCACATAGTTAAAATGAACATGTTAGAAATGATTTTTTTATGCGTATTTTAATAAAATAAATAATTTTTGGACCAAACGTATTGTTTAATATTCCAACTATTTTTCCATTATAAATATACACTCCTTCTCCTCTAAAACCATCAATAATACTTTCTCTCCTCTAAAAGAATAAGAAAGAAAAATGACAAGCAGTGAGGTCATCTCTACGACAACAGGGCAAAGGAGGTAATCAAAAAACAATATTTTAGCTAAACTTTTCTTTTATATATATATAAATCTATTTGAACATCTTCTAAGTTTTATATCCATAATTAATATTAATTTGCAATTTTTTTTATTTTTTATTTTTTTGTCAACAATTCCAGTGTTAAGCAAGTTATTCAGGGTTTTTTTCTTTTTTCTTTTTTAATTCCAAAATAAAATATTGAAAAACATGAAGTCGTTTGAAAATCAATTTTGGGAAATAATTATATTAATTATAGCTAGGGTCATCTTTGCTAGTATTTGATATTGAGATGTTGATAAAAAATCTTGGCTTTTGAATCACTTGTCTATTGATGACTTTATGTCCATTTTAATCCAAAGGAAAAGAAAAAACAAACAAACAAGCAAGCTTTGTATTTCTCTAGTTTATTTGGTTCATATTCTAATTCCTTGTGTGTGATCCATTAGACTAGAAGGAAAGGTGGCAATAATAACCGGCGGTGCAAGCGGGATTGGCGAATGCACTGCAAAACTCTTTGTCCACCATGGTGCCAAAGTAGTCGTCGCAGACATCCAAGACGACCTCGGTCGTGCATTGTGTGCCAACGTGCTCGGCTCGACCAACTCCCTCTATGTTCACTGCGATGTCACAGACGAATCCCAAGTCCAAGCGGCAGTGGCAGCTGCAGTAGAAACCTTCGGCAAGCTCGACATCATGATGAATAATGCAGGCATAGCCGACCCCAGCAAGCCTCGAATCATCGACAACGATAAACAAGATTTCGATCGAGTCCTCAGCGTCAACATCACAGGTGTCTTCCTCGGCATCAAACATGCAGCACAGGCCATGATCCCAGTCAAAACTGGCTCGATTTTATCGACCGCAAGCGTGGCATCGTATATCGGTGGCTCTGCATCGCACGCTTACACATGTTCGAAGCACGCAGTGGTAGGGCTAACGAAGAATGCCGCGGTGGAGTTGGGGCAGTTTGGGATTAGGGTAAATTGTTTGTCGCCTTATGTGTTGGCGACGCCGTTAGCAACGGAGTATGTGGATTTGGACGGGGCGGAGTTTGAGAAGTATGCGAGTTCTAGGGCAAATTTGAAAGGAGTAACACTTAAGGCTGAAGATGTGGCTAATGCAGCCTTGTTTTTGGCAAGTGATGAAAGTAGGTATGTGAGTGGCCATAATTTGTTTGTTGATGGAGGTTTTAGTATTGTTAATCCTAATATCCAAATCTTCCAATACACTCAATAG

mRNA sequence

ATGATCCCAGCTAAAACCGGCTCGATTATAACAACAGGGAACGTGGCATCAAATATCGGCGGTGCGGCGTCACACGCTTACACATGCTCGAAGCATGCGGTAGTGGGGTTGATGAAGAACGCGGCGGGAGAGTTGGGGCAGTTCGGGATTAGGGCAAATTTGAAAGGAGTGACGCTTAAGGTTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGTGATGAAAGTAGACTAGAAGGAAAGGTGGCAATAATAACCGGCGGTGCAAGCGGGATTGGCGAATGCACTGCAAAACTCTTTGTCCACCATGGTGCCAAAGTAGTCGTCGCAGACATCCAAGACGACCTCGGTCGTGCATTGTGTGCCAACGTGCTCGGCTCGACCAACTCCCTCTATGTTCACTGCGATGTCACAGACGAATCCCAAGTCCAAGCGGCAGTGGCAGCTGCAGTAGAAACCTTCGGCAAGCTCGACATCATGATGAATAATGCAGGCATAGCCGACCCCAGCAAGCCTCGAATCATCGACAACGATAAACAAGATTTCGATCGAGTCCTCAGCGTCAACATCACAGGTGTCTTCCTCGGCATCAAACATGCAGCACAGGCCATGATCCCAGTCAAAACTGGCTCGATTTTATCGACCGCAAGCGTGGCATCGTATATCGGTGGCTCTGCATCGCACGCTTACACATGTTCGAAGCACGCAGTGGTAGGGCTAACGAAGAATGCCGCGGTGGAGTTGGGGCAGTTTGGGATTAGGGTAAATTGTTTGTCGCCTTATGTGTTGGCGACGCCGTTAGCAACGGAGTATGTGGATTTGGACGGGGCGGAGTTTGAGAAGTATGCGAGTTCTAGGGCAAATTTGAAAGGAGTAACACTTAAGGCTGAAGATGTGGCTAATGCAGCCTTGTTTTTGGCAAGTGATGAAAGTAGGTATGTGAGTGGCCATAATTTGTTTGTTGATGGAGGTTTTAGTATTGTTAATCCTAATATCCAAATCTTCCAATACACTCAATAG

Coding sequence (CDS)

ATGATCCCAGCTAAAACCGGCTCGATTATAACAACAGGGAACGTGGCATCAAATATCGGCGGTGCGGCGTCACACGCTTACACATGCTCGAAGCATGCGGTAGTGGGGTTGATGAAGAACGCGGCGGGAGAGTTGGGGCAGTTCGGGATTAGGGCAAATTTGAAAGGAGTGACGCTTAAGGTTGAAGATGTGGCCAATGCAGCTTTGTTTTTGGCAAGTGATGAAAGTAGACTAGAAGGAAAGGTGGCAATAATAACCGGCGGTGCAAGCGGGATTGGCGAATGCACTGCAAAACTCTTTGTCCACCATGGTGCCAAAGTAGTCGTCGCAGACATCCAAGACGACCTCGGTCGTGCATTGTGTGCCAACGTGCTCGGCTCGACCAACTCCCTCTATGTTCACTGCGATGTCACAGACGAATCCCAAGTCCAAGCGGCAGTGGCAGCTGCAGTAGAAACCTTCGGCAAGCTCGACATCATGATGAATAATGCAGGCATAGCCGACCCCAGCAAGCCTCGAATCATCGACAACGATAAACAAGATTTCGATCGAGTCCTCAGCGTCAACATCACAGGTGTCTTCCTCGGCATCAAACATGCAGCACAGGCCATGATCCCAGTCAAAACTGGCTCGATTTTATCGACCGCAAGCGTGGCATCGTATATCGGTGGCTCTGCATCGCACGCTTACACATGTTCGAAGCACGCAGTGGTAGGGCTAACGAAGAATGCCGCGGTGGAGTTGGGGCAGTTTGGGATTAGGGTAAATTGTTTGTCGCCTTATGTGTTGGCGACGCCGTTAGCAACGGAGTATGTGGATTTGGACGGGGCGGAGTTTGAGAAGTATGCGAGTTCTAGGGCAAATTTGAAAGGAGTAACACTTAAGGCTGAAGATGTGGCTAATGCAGCCTTGTTTTTGGCAAGTGATGAAAGTAGGTATGTGAGTGGCCATAATTTGTTTGTTGATGGAGGTTTTAGTATTGTTAATCCTAATATCCAAATCTTCCAATACACTCAATAG

Protein sequence

MIPAKTGSIITTGNVASNIGGAASHAYTCSKHAVVGLMKNAAGELGQFGIRANLKGVTLKVEDVANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYVSGHNLFVDGGFSIVNPNIQIFQYTQ*
Homology
BLAST of Chy4G086120 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.6e-90
Identity = 167/275 (60.73%), Postives = 215/275 (78.18%), Query Frame = 0

Query: 64  VANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCAN 123
           +A  +  L +   RLEGKVA+ITGGASGIGE TAKLF  HGAKV +AD+QD+LG ++   
Sbjct: 1   MAATSQVLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSV-VE 60

Query: 124 VLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFD 183
            +G++NS Y+HCDVT+E  V+ AV   V T+GKLDIM +NAGI+DP++PRIIDN+K DF+
Sbjct: 61  AIGTSNSTYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFE 120

Query: 184 RVLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKN 243
           RV SVN+TGVFL +KHAA+ MIP ++G+I+STAS++S +GG +SHAY  SKHAV+GLT+N
Sbjct: 121 RVFSVNVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRN 180

Query: 244 AAVELGQFGIRVNCLSPYVLATPLATEYVDL-DGAEFEKYASSRANLKGVTLKAEDVANA 303
            AVELGQFGIRVNCLSP+ L T L  ++  + +  EFE   +   NLKG     EDVANA
Sbjct: 181 LAVELGQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANA 240

Query: 304 ALFLASDESRYVSGHNLFVDGGFSIVNPNIQIFQY 338
           AL+LASDE++YVSGHNLF+DGGFS+ N  I++FQY
Sbjct: 241 ALYLASDEAKYVSGHNLFIDGGFSVCNSVIKVFQY 274

BLAST of Chy4G086120 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 8.6e-81
Identity = 160/274 (58.39%), Postives = 203/274 (74.09%), Query Frame = 0

Query: 64  VANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCAN 123
           +A  +  +++D  +L GKVA+ITGGASGIG CTA+LFV HGA+VVVADIQD+LG +L A 
Sbjct: 1   MAAGSSHVSADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAE 60

Query: 124 VLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFD 183
            LG   S YVHCDVT+E  V AAV  AV  FGKLD+M NNAG++ P   R+ +  K+DF+
Sbjct: 61  -LGPDASSYVHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFE 120

Query: 184 RVLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKN 243
           RVL+VN+ G FLG KHAA+ M P + GSI+STAS++S + G+ASHAYT SKHA+VG T+N
Sbjct: 121 RVLAVNLVGPFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTEN 180

Query: 244 AAVELGQFGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGV-TLKAEDVANA 303
           AA ELG+ GIRVNC+SP  +ATPLA   + +D    E   ++ ANLKG   LKA+D+A A
Sbjct: 181 AAGELGRHGIRVNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAA 240

Query: 304 ALFLASDESRYVSGHNLFVDGGFSIVNPNIQIFQ 337
           ALFLASD+ RYVSG NL VDGG S+VN +   F+
Sbjct: 241 ALFLASDDGRYVSGQNLRVDGGLSVVNSSFGFFR 273

BLAST of Chy4G086120 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.6e-71
Identity = 137/260 (52.69%), Postives = 179/260 (68.85%), Query Frame = 0

Query: 72  ASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSL 131
           AS  +RL+ KVAIITGGA GIGE TAKLFV +GAKVV+ADI DD G+ +C N+       
Sbjct: 8   ASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVIS 67

Query: 132 YVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNIT 191
           +VHCDVT +  V+  V   +   GKLDIM  N G+   +   I++   +DF RV+ +N+ 
Sbjct: 68  FVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVY 127

Query: 192 GVFLGIKHAAQAMIPVKTGSILSTASVASYIGG-SASHAYTCSKHAVVGLTKNAAVELGQ 251
           G FL  KHAA+ MIP K GSI+ TAS++S+  G   SH YT +KHAV+GLT +   ELGQ
Sbjct: 128 GAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQ 187

Query: 252 FGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDE 311
            GIRVNC+SPYV+A+PL T+   +D +  E+ A   ANLKG+ L+AEDVA+A  +LA DE
Sbjct: 188 HGIRVNCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGDE 247

Query: 312 SRYVSGHNLFVDGGFSIVNP 331
           S+YVSG NL +DGG++  NP
Sbjct: 248 SKYVSGLNLVIDGGYTRTNP 267

BLAST of Chy4G086120 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.6e-71
Identity = 138/266 (51.88%), Postives = 181/266 (68.05%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVHCD 136
           RLEGKVA++TGGASGIGE  A+LF+ HGAK+ + D+QD+LG+ +   + G  ++ Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 137 VTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVFLG 196
           VT E  V+ AV    E +G +DIM+NNAGI       I D D  +F +V  +N+ GVFLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 197 IKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 256
           +KHAA+ MIP   GSI+S ASV+S I G+  H YT +KHAVVGLTK+ A ELG+ GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 257 CLSPYVLATPLATEYVDLDGAE------FEKYASSRANLKGVTLKAEDVANAALFLASDE 316
           C+SPY + T L+  Y+     +      F  +  S ANLKGV L   DVA A L+LA++E
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLMPNDVAEAVLYLATEE 241

Query: 317 SRYVSGHNLFVDGGFSIVNPNIQIFQ 337
           S+YVSG NL +DGGFSI N  +Q+F+
Sbjct: 242 SKYVSGLNLVIDGGFSIANHTLQVFE 267

BLAST of Chy4G086120 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 134/259 (51.74%), Postives = 178/259 (68.73%), Query Frame = 0

Query: 73  SDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLY 132
           S  +RL+ KVAIITGGA GIGE TAKLFV +GAKVV+ADI DD G+ +C N+       +
Sbjct: 9   SSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISF 68

Query: 133 VHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITG 192
           VHCDVT +  V+  V   +   GKLDIM  N G+   +   I++   +DF RV+ +N+ G
Sbjct: 69  VHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYG 128

Query: 193 VFLGIKHAAQAMIPVKTGSILSTASVASYIGG-SASHAYTCSKHAVVGLTKNAAVELGQF 252
            FL  KHAA+ MIP K GSI+ TAS++S+  G   SH YT +KHAV+GLT +   ELG++
Sbjct: 129 AFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEY 188

Query: 253 GIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDES 312
           GIRVNC+SPY++A+PL T+   +D +  E+ A   ANLKG  L+AEDVA+A  +LA DES
Sbjct: 189 GIRVNCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGDES 248

Query: 313 RYVSGHNLFVDGGFSIVNP 331
           +YVSG NL +DGG++  NP
Sbjct: 249 KYVSGLNLVIDGGYTRTNP 267

BLAST of Chy4G086120 vs. ExPASy TrEMBL
Match: A0A0A0LGR0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778270 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.7e-140
Identity = 258/265 (97.36%), Postives = 262/265 (98.87%), Query Frame = 0

Query: 75  ESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH 134
           + RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH
Sbjct: 13  QRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH 72

Query: 135 CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVF 194
           CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDK DFDRVLS+N+TGVF
Sbjct: 73  CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDFDRVLSINVTGVF 132

Query: 195 LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 254
           LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR
Sbjct: 133 LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 192

Query: 255 VNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV 314
           VNCLSPYVLATPLATE+VDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV
Sbjct: 193 VNCLSPYVLATPLATEFVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV 252

Query: 315 SGHNLFVDGGFSIVNPNIQIFQYTQ 340
           SGHNLFVDGGFSIVNPNIQIFQY Q
Sbjct: 253 SGHNLFVDGGFSIVNPNIQIFQYPQ 277

BLAST of Chy4G086120 vs. ExPASy TrEMBL
Match: A0A5A7TKJ5 (Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004260 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.0e-129
Identity = 235/272 (86.40%), Postives = 254/272 (93.38%), Query Frame = 0

Query: 65  ANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANV 124
           ++  + L + + RLEGKVA+ITGGASGIGECTAK+FVHHGAKVV+ADIQDDLG ALCANV
Sbjct: 3   SSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCANV 62

Query: 125 LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDR 184
           LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLD+MMNNAGI DPSKPRIIDND+ DFDR
Sbjct: 63  LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDFDR 122

Query: 185 VLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 244
           VL++N+TGVFLGIKHAAQAMIP KTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA
Sbjct: 123 VLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 182

Query: 245 AVELGQFGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAAL 304
           AVELGQFGIRVNCLSPY L TPLAT++V L+GAEFE+   S+ANLKGVTLKAEDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANAAL 242

Query: 305 FLASDESRYVSGHNLFVDGGFSIVNPNIQIFQ 337
           FLASDESRYVSGHNL +DGGFSIVNPNI IFQ
Sbjct: 243 FLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

BLAST of Chy4G086120 vs. ExPASy TrEMBL
Match: A0A1S3B7H5 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.0e-129
Identity = 235/272 (86.40%), Postives = 254/272 (93.38%), Query Frame = 0

Query: 65  ANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANV 124
           ++  + L + + RLEGKVA+ITGGASGIGECTAK+FVHHGAKVV+ADIQDDLG ALCANV
Sbjct: 3   SSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCANV 62

Query: 125 LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDR 184
           LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLD+MMNNAGI DPSKPRIIDND+ DFDR
Sbjct: 63  LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDFDR 122

Query: 185 VLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 244
           VL++N+TGVFLGIKHAAQAMIP KTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA
Sbjct: 123 VLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 182

Query: 245 AVELGQFGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAAL 304
           AVELGQFGIRVNCLSPY L TPLAT++V L+GAEFE+   S+ANLKGVTLKAEDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANAAL 242

Query: 305 FLASDESRYVSGHNLFVDGGFSIVNPNIQIFQ 337
           FLASDESRYVSGHNL +DGGFSIVNPNI IFQ
Sbjct: 243 FLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

BLAST of Chy4G086120 vs. ExPASy TrEMBL
Match: A0A0A0LEP1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778280 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 3.7e-127
Identity = 235/275 (85.45%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 65  ANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANV 124
           +N  +   + + RLEGKVA+ITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG ALCANV
Sbjct: 3   SNEIISTTTWQRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCANV 62

Query: 125 LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDR 184
           LGSTNSLYVHCDVT+ESQ      AAVETFGKLDIMMNNAGIADPSKPRIIDNDK DFDR
Sbjct: 63  LGSTNSLYVHCDVTEESQ------AAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDFDR 122

Query: 185 VLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 244
           VLS+N+TGVFLGIKHAAQAMIP KTGSILSTASVASY GGSASHAYTCSKHAVVGLTKNA
Sbjct: 123 VLSINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYTGGSASHAYTCSKHAVVGLTKNA 182

Query: 245 AVELGQFGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAAL 304
           AVELGQFGIRVNCLSP+ L TPLAT++V LDG EFEK   S+ANLKGVTLKAEDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPFALVTPLATKFVGLDGPEFEKIMGSKANLKGVTLKAEDVANAAL 242

Query: 305 FLASDESRYVSGHNLFVDGGFSIVNPNIQIFQYTQ 340
           FLASDESRYVSGHNLF+DGGFSIVNPNI IFQY Q
Sbjct: 243 FLASDESRYVSGHNLFIDGGFSIVNPNIDIFQYPQ 271

BLAST of Chy4G086120 vs. ExPASy TrEMBL
Match: A0A6J1J123 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481719 PE=4 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 6.8e-121
Identity = 219/264 (82.95%), Postives = 242/264 (91.67%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGS-TNSLYVHC 136
           RLEGKVAIITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG+ALCA  LG  TN LYVHC
Sbjct: 14  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATALGGPTNCLYVHC 73

Query: 137 DVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVFL 196
           DVTDE+Q++AAV AAVE FGKLDIMMNNAGIADPSKPRIIDN K+DF+RVL++N+TGVFL
Sbjct: 74  DVTDEAQIKAAVEAAVEAFGKLDIMMNNAGIADPSKPRIIDNHKEDFERVLNINVTGVFL 133

Query: 197 GIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV 256
           GIKHAAQAMIPVKTGSI+STAS+ASY+GGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV
Sbjct: 134 GIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV 193

Query: 257 NCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYVS 316
           NCLSP+ + TPLAT +V L+G EFE   +S ANLKGVTL+ EDVANAALFLASDESRYVS
Sbjct: 194 NCLSPFAMVTPLATRFVGLEGEEFETAMNSMANLKGVTLRTEDVANAALFLASDESRYVS 253

Query: 317 GHNLFVDGGFSIVNPNIQIFQYTQ 340
           GHNL +DGGF I NPNI+IF+Y +
Sbjct: 254 GHNLLIDGGFGITNPNIKIFEYPE 277

BLAST of Chy4G086120 vs. NCBI nr
Match: XP_004136700.3 (secoisolariciresinol dehydrogenase [Cucumis sativus])

HSP 1 Score: 514 bits (1324), Expect = 4.40e-182
Identity = 261/265 (98.49%), Postives = 263/265 (99.25%), Query Frame = 0

Query: 75  ESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH 134
           + RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH
Sbjct: 13  QRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH 72

Query: 135 CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVF 194
           CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVF
Sbjct: 73  CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVF 132

Query: 195 LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 254
           LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR
Sbjct: 133 LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 192

Query: 255 VNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV 314
           VNCLSPYVLATPLATE+VDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV
Sbjct: 193 VNCLSPYVLATPLATEFVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV 252

Query: 315 SGHNLFVDGGFSIVNPNIQIFQYTQ 339
           SGHNLFVDGGFSIVNPNIQIFQY Q
Sbjct: 253 SGHNLFVDGGFSIVNPNIQIFQYPQ 277

BLAST of Chy4G086120 vs. NCBI nr
Match: KAE8651098.1 (hypothetical protein Csa_002484 [Cucumis sativus])

HSP 1 Score: 512 bits (1318), Expect = 3.74e-181
Identity = 259/265 (97.74%), Postives = 262/265 (98.87%), Query Frame = 0

Query: 75  ESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH 134
           + RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH
Sbjct: 13  QRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVH 72

Query: 135 CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVF 194
           CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVF
Sbjct: 73  CDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVF 132

Query: 195 LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 254
           LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR
Sbjct: 133 LGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIR 192

Query: 255 VNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV 314
           VNCLSPYVLATPLATE+VDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV
Sbjct: 193 VNCLSPYVLATPLATEFVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYV 252

Query: 315 SGHNLFVDGGFSIVNPNIQIFQYTQ 339
           SGHNLF+DGGFSIVNPNI IFQY Q
Sbjct: 253 SGHNLFIDGGFSIVNPNIDIFQYPQ 277

BLAST of Chy4G086120 vs. NCBI nr
Match: XP_004136701.3 (secoisolariciresinol dehydrogenase isoform X1 [Cucumis sativus])

HSP 1 Score: 481 bits (1238), Expect = 5.69e-169
Identity = 240/275 (87.27%), Postives = 254/275 (92.36%), Query Frame = 0

Query: 65  ANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANV 124
           +N  +   + + RLEGKVA+ITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG ALCANV
Sbjct: 3   SNEIISTTTWQRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCANV 62

Query: 125 LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDR 184
           LGSTNSLYVHCDVT+ESQVQ AVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDK DFDR
Sbjct: 63  LGSTNSLYVHCDVTEESQVQEAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDFDR 122

Query: 185 VLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 244
           VLS+N+TGVFLGIKHAAQAMIP KTGSILSTASVASY GGSASHAYTCSKHAVVGLTKNA
Sbjct: 123 VLSINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYTGGSASHAYTCSKHAVVGLTKNA 182

Query: 245 AVELGQFGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAAL 304
           AVELGQFGIRVNCLSP+ L TPLAT++V LDG EFEK   S+ANLKGVTLKAEDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPFALVTPLATKFVGLDGPEFEKIMGSKANLKGVTLKAEDVANAAL 242

Query: 305 FLASDESRYVSGHNLFVDGGFSIVNPNIQIFQYTQ 339
           FLASDESRYVSGHNLF+DGGFSIVNPNI IFQY Q
Sbjct: 243 FLASDESRYVSGHNLFIDGGFSIVNPNIDIFQYPQ 277

BLAST of Chy4G086120 vs. NCBI nr
Match: XP_031737878.1 (secoisolariciresinol dehydrogenase isoform X2 [Cucumis sativus])

HSP 1 Score: 480 bits (1236), Expect = 9.92e-169
Identity = 239/263 (90.87%), Postives = 249/263 (94.68%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVHCD 136
           RLEGKVA+ITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG ALCANVLGSTNSLYVHCD
Sbjct: 11  RLEGKVALITGGASGIGECTAKLFVHHGAKVVIADIQDDLGHALCANVLGSTNSLYVHCD 70

Query: 137 VTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVFLG 196
           VT+ESQVQ AVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDK DFDRVLS+N+TGVFLG
Sbjct: 71  VTEESQVQEAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDFDRVLSINVTGVFLG 130

Query: 197 IKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 256
           IKHAAQAMIP KTGSILSTASVASY GGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVN
Sbjct: 131 IKHAAQAMIPAKTGSILSTASVASYTGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 190

Query: 257 CLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYVSG 316
           CLSP+ L TPLAT++V LDG EFEK   S+ANLKGVTLKAEDVANAALFLASDESRYVSG
Sbjct: 191 CLSPFALVTPLATKFVGLDGPEFEKIMGSKANLKGVTLKAEDVANAALFLASDESRYVSG 250

Query: 317 HNLFVDGGFSIVNPNIQIFQYTQ 339
           HNLF+DGGFSIVNPNI IFQY Q
Sbjct: 251 HNLFIDGGFSIVNPNIDIFQYPQ 273

BLAST of Chy4G086120 vs. NCBI nr
Match: XP_008442883.1 (PREDICTED: secoisolariciresinol dehydrogenase-like [Cucumis melo] >KAA0043844.1 secoisolariciresinol dehydrogenase-like [Cucumis melo var. makuwa] >TYK25290.1 secoisolariciresinol dehydrogenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 476 bits (1224), Expect = 6.91e-167
Identity = 235/272 (86.40%), Postives = 254/272 (93.38%), Query Frame = 0

Query: 65  ANAALFLASDESRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANV 124
           ++  + L + + RLEGKVA+ITGGASGIGECTAK+FVHHGAKVV+ADIQDDLG ALCANV
Sbjct: 3   SSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCANV 62

Query: 125 LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDR 184
           LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLD+MMNNAGI DPSKPRIIDND+ DFDR
Sbjct: 63  LGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDFDR 122

Query: 185 VLSVNITGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 244
           VL++N+TGVFLGIKHAAQAMIP KTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA
Sbjct: 123 VLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNA 182

Query: 245 AVELGQFGIRVNCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAAL 304
           AVELGQFGIRVNCLSPY L TPLAT++V L+GAEFE+   S+ANLKGVTLKAEDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANAAL 242

Query: 305 FLASDESRYVSGHNLFVDGGFSIVNPNIQIFQ 336
           FLASDESRYVSGHNL +DGGFSIVNPNI IFQ
Sbjct: 243 FLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

BLAST of Chy4G086120 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 8.6e-68
Identity = 132/269 (49.07%), Postives = 177/269 (65.80%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVL---GSTNSLYV 136
           RL GKVA+ITGGA+GIGE   +LF  HGAKV + D+QDDLG  +C ++L       + ++
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 137 HCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGV 196
           H DV  E  +  AV  AV+ FG LDI++NNAG+     P I +    +F+    VN+ G 
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 197 FLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGI 256
           FL +KHAA+ MIP K GSI+S  SV   +GG   H+Y  SKHAV+GLT++ A ELGQ GI
Sbjct: 137 FLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGI 196

Query: 257 RVNCLSPYVLATPLATEYVDLDG------AEFEKYASSRANLKGVTLKAEDVANAALFLA 316
           RVNC+SPY +AT LA  ++  +         F  +A++ ANLKGV L  +DVANA LFLA
Sbjct: 197 RVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVANAVLFLA 256

Query: 317 SDESRYVSGHNLFVDGGFSIVNPNIQIFQ 337
           SD+SRY+SG NL +DGGF+  N + ++F+
Sbjct: 257 SDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

BLAST of Chy4G086120 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 3.1e-65
Identity = 136/263 (51.71%), Postives = 175/263 (66.54%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNS---LYV 136
           RLEGKVAIITGGA GIG+ T  LF  HGA VV+AD+ +  G +L  ++     S    ++
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 137 HCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPR--IIDNDKQDFDRVLSVNIT 196
            CDV+ E+ V+  V   V  +G+LDI+ NNAG+    K    I+D D  +FD V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 197 GVFLGIKHAAQAMIPVK-TGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQ 256
           GV LG+KH A+AMI     G I+STASVA  +GG   HAYT SKHA+VGLTKNAA ELG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 257 FGIRVNCLSPYVLATPLAT---------EYVDLDGAEFEKYASSRANLKGVTLKAEDVAN 316
           +GIRVNC+SP+ +AT +           +  D D  E E++  S ANLKG TL+A D+A 
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGETLRANDIAE 270

Query: 317 AALFLASDESRYVSGHNLFVDGG 325
           AAL+LASDES+YV+GHNL VDGG
Sbjct: 271 AALYLASDESKYVNGHNLVVDGG 293

BLAST of Chy4G086120 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.0e-64
Identity = 127/255 (49.80%), Postives = 179/255 (70.20%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVHCD 136
           RL+GK+ IITGGASGIG  + +LF  HGA+VV+ D+QD+LG+ +  ++ G   + Y HCD
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSI-GEDKASYYHCD 64

Query: 137 VTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVFLG 196
           VT+E++V+ AV   VE +GKLD++ +NAG+ +P    I+D +  + DR +++N+ G    
Sbjct: 65  VTNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTAAF 124

Query: 197 IKHAAQAMIPVK-TGSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV 256
           IKHAA+AM+     GSI+ T SVA+ I G+A H YT SKH ++GL K+A+  LG++GIRV
Sbjct: 125 IKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRV 184

Query: 257 NCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYVS 316
           N ++P+ +ATPL      ++    E+  S+ ANLKG+ LKA  VA AALFLASDES YVS
Sbjct: 185 NGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAYVS 244

Query: 317 GHNLFVDGGFSIVNP 331
           G NL VDGG+S+V P
Sbjct: 245 GQNLAVDGGYSVVKP 257

BLAST of Chy4G086120 vs. TAIR 10
Match: AT2G47120.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 126/256 (49.22%), Postives = 182/256 (71.09%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVHCD 136
           RLEGK+ IITGGASGIG   A+LF  HGAKVV+ D+Q++LG+ + A ++G   + +  CD
Sbjct: 5   RLEGKIVIITGGASGIGADAARLFTDHGAKVVIVDVQEELGQNV-AVLIGKDKASFYRCD 64

Query: 137 VTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVFLG 196
           VT+E++V+ AV   VE  GKLD++ +NAG+ +P +   +D D + FDR+++VN+ G    
Sbjct: 65  VTNETEVEDAVKFTVEKHGKLDVLFSNAGVLEPLE-SFLDFDLERFDRIMAVNVRGAAAF 124

Query: 197 IKHAAQAMIPVKT-GSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV 256
           IKHAA+AM+   T GSI+ T SV++ IGG   H YT SKH +VGL ++A  +LG++GIRV
Sbjct: 125 IKHAARAMVEKGTRGSIVCTTSVSAEIGG-GHHGYTASKHGLVGLIRSACGDLGKYGIRV 184

Query: 257 NCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYVS 316
           N ++PY +ATP+ T + ++ G + E Y  ++  LKG+ LKA  VA  ALFLASD+S Y+S
Sbjct: 185 NGVAPYAVATPM-TSHDEVTGKQLEDYFDAKGILKGMVLKASHVAQVALFLASDDSAYIS 244

Query: 317 GHNLFVDGGFSIVNPN 332
           G NL VDGG+++V P+
Sbjct: 245 GQNLAVDGGYTVVKPS 256

BLAST of Chy4G086120 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 8.3e-63
Identity = 126/253 (49.80%), Postives = 176/253 (69.57%), Query Frame = 0

Query: 77  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCANVLGSTNSLYVHCD 136
           RL+GK+AIITGGASGIG    +LF  HGAKVV+ DIQ++LG+ L  ++ G   + +  C+
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSI-GLDKASFYRCN 64

Query: 137 VTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKQDFDRVLSVNITGVFLG 196
           VTDE+ V+ AV   VE  GKLD++ +NAG+ + +   ++D D + FDR ++VN+ G    
Sbjct: 65  VTDETDVENAVKFTVEKHGKLDVLFSNAGVLE-AFGSVLDLDLEAFDRTMAVNVRGAAAF 124

Query: 197 IKHAAQAMIPVKT-GSILSTASVASYIGGSASHAYTCSKHAVVGLTKNAAVELGQFGIRV 256
           IKHAA++M+   T GSI+ T S+A+ IGG   H+YT SKHA++GL ++A   LGQ+GIRV
Sbjct: 125 IKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRV 184

Query: 257 NCLSPYVLATPLATEYVDLDGAEFEKYASSRANLKGVTLKAEDVANAALFLASDESRYVS 316
           N ++PY +AT + + Y +      E+Y  +  NLKGV LKA  +A AALFLASD+S Y+S
Sbjct: 185 NGVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASDDSVYIS 244

Query: 317 GHNLFVDGGFSIV 329
           G NL VDGGFS+V
Sbjct: 245 GQNLVVDGGFSVV 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94KL74.6e-9060.73Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
Q7FAE18.6e-8158.39Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
A3F5F01.6e-7152.69Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
F1SWA03.6e-7151.88Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL81.4e-7051.74Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LGR01.7e-14097.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778270 PE=4 SV=1[more]
A0A5A7TKJ51.0e-12986.40Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3B7H51.0e-12986.40secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 ... [more]
A0A0A0LEP13.7e-12785.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778280 PE=4 SV=1[more]
A0A6J1J1236.8e-12182.95secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481... [more]
Match NameE-valueIdentityDescription
XP_004136700.34.40e-18298.49secoisolariciresinol dehydrogenase [Cucumis sativus][more]
KAE8651098.13.74e-18197.74hypothetical protein Csa_002484 [Cucumis sativus][more]
XP_004136701.35.69e-16987.27secoisolariciresinol dehydrogenase isoform X1 [Cucumis sativus][more]
XP_031737878.19.92e-16990.87secoisolariciresinol dehydrogenase isoform X2 [Cucumis sativus][more]
XP_008442883.16.91e-16786.40PREDICTED: secoisolariciresinol dehydrogenase-like [Cucumis melo] >KAA0043844.1 ... [more]
Match NameE-valueIdentityDescription
AT1G52340.18.6e-6849.07NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G51680.13.1e-6551.71NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47140.12.0e-6449.80NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47120.11.3e-6349.22NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.28.3e-6349.80NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 204..220
score: 29.86
coord: 289..309
score: 34.88
coord: 230..249
score: 42.87
coord: 251..268
score: 37.28
coord: 155..166
score: 57.66
coord: 82..99
score: 48.2
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 230..249
score: 37.39
coord: 155..166
score: 50.92
coord: 210..218
score: 42.2
NoneNo IPR availableGENE3D3.40.50.720coord: 77..336
e-value: 2.0E-82
score: 278.3
NoneNo IPR availableGENE3D3.40.50.720coord: 1..76
e-value: 2.4E-14
score: 55.2
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 87..326
e-value: 3.1E-69
score: 233.1
coord: 4..53
e-value: 8.8E-9
score: 35.3
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 77..329
e-value: 1.3E-22
score: 78.0
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 84..270
e-value: 3.4E-17
score: 60.4
NoneNo IPR availablePANTHERPTHR43180:SF50SECOISOLARICIRESINOL DEHYDROGENASE-LIKEcoord: 1..53
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 52..77
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 1..53
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 76..336
NoneNo IPR availablePANTHERPTHR43180:SF50SECOISOLARICIRESINOL DEHYDROGENASE-LIKEcoord: 76..336
NoneNo IPR availablePANTHERPTHR43180:SF50SECOISOLARICIRESINOL DEHYDROGENASE-LIKEcoord: 52..77
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 217..245
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 14..42
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 79..328
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 1..77

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G086120.1Chy4G086120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity