Chy4G084330 (gene) Cucumber (hystrix) v1

BLAST of Chy4G084330 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 226.1 bits (575), Expect = 1.6e-57
Identity = 190/734 (25.89%), Postives = 321/734 (43.73%), Query Frame = 0

Query: 24  KRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEV--FKQKDI 83
           +R+    S+++AVL DA+EKQ  ++ L NWL +L    Y+V++ +DE+K +   F Q + 
Sbjct: 32  QRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAATYEVDDILDEYKTKATRFLQSEY 91

Query: 84  GKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETTHTF 143
           G+          ++   + K   +  Q   +LN IA    +FHL+ K I+  +  T  T 
Sbjct: 92  GRY-------HPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQEKIIERQA-ATRETG 151

Query: 144 PSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILNDFNKCAHVFHIVGEAGIGKSTVA 203
              +E  +          Y       +   I     +D  K + V  I+G  G+GK+T++
Sbjct: 152 SVLTEPQV----------YGRDKEKDEIVKILINTASDAQKLS-VLPILGMGGLGKTTLS 211

Query: 204 RFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLL 263
           + ++N   V      + W+C+ + FN  RL+K +    + K +  D+    L  K++ LL
Sbjct: 212 QMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSL-SDMDLAPLQKKLQELL 271

Query: 264 KVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQKTER 323
             + + +V  D  + N D      L  +LK+G  G+ ++ TT+ E++             
Sbjct: 272 NGKRYFLVLDD--VWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKVG------------ 331

Query: 324 RSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNLKKNPVAGDR 383
                                                                       
Sbjct: 332 ------------------------------------------------------------ 391

Query: 384 ESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQSSSRL 443
                                     ++   + P            ++L  LS +    L
Sbjct: 392 --------------------------SIMGTLQP------------YELSNLSPEDCWFL 451

Query: 444 FKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQEK-LS 503
           F + AF +++E+ NP L  I  ++++KC GVPLA K LG +L  K    EW ++++  + 
Sbjct: 452 FMQRAFGHQEEI-NPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREEREWEHVRDSPIW 511

Query: 504 LQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQLWMANGFLHSPEE 563
              + E+ IL  LR+ Y  +P  L+ CF++C+    D  ++  ++I  WMA+GFL S  +
Sbjct: 512 NLPQDESSILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLLS--K 571

Query: 564 KNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGNN- 623
            N  +E++G + + EL+ R F QEIE      + K+H LI  LA  +    +  S     
Sbjct: 572 GNLELEDVGNEVWNELYLRSFFQEIEVESGKTYFKMHDLIHDLATSLFSANTSSSNIREI 625

Query: 624 HPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLRLLYLSNADLQEIPNSIGTLKYLR 683
           + N    + SI F E       +S +   +  +  LR+L L N++L ++P+SIG L +LR
Sbjct: 632 NANYDGYMMSIGFAE-----VVSSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLR 625

Query: 684 YLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLD 743
           YLDL GN +I++LP  +C LQ+LQTL L  C +L  LPK    L SLR L +    L   
Sbjct: 692 YLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTST 625

Query: 744 KNGVGTMNSLRFLA 754
              +G +  L+ L+
Sbjct: 752 PPRIGLLTCLKSLS 625

BLAST of Chy4G084330 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 3.3e-55
Identity = 185/712 (25.98%), Postives = 312/712 (43.82%), Query Frame = 0

Query: 24  KRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEV--FKQKDI 83
           +R+    S+++AVL DA+EKQ  N+ L NWL +L    Y+V++ +DE+K +   F Q + 
Sbjct: 32  QRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDEYKTKATRFSQSEY 91

Query: 84  GKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETTHTF 143
           G+          ++   + K   +  Q   +L  IA     FHL  K ++          
Sbjct: 92  GRY-------HPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHLHEKIVERQ-------- 151

Query: 144 PSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILNDFNKCAH--VFHIVGEAGIGKST 203
                 ++R    S      +    ++ D I   ++N+ +   H  V  I+G  G+GK+T
Sbjct: 152 ------AVRRETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQHLSVLPILGMGGLGKTT 211

Query: 204 VARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQLLSKVKR 263
           +A+ ++N   V  +  S+ W+CV E F+  RL+K +    + + +  ++    L  K++ 
Sbjct: 212 LAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQE 271

Query: 264 LLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQKT 323
           LL  + +L+V  D  + N D +    L  +LK+G  G+ ++ TT+ E++           
Sbjct: 272 LLNGKRYLLVLDD--VWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVG---------- 331

Query: 324 ERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNLKKNPVAG 383
                                                                       
Sbjct: 332 ------------------------------------------------------------ 391

Query: 384 DRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQSSS 443
                                       ++   + P            ++L  LS++   
Sbjct: 392 ----------------------------SIMGTLQP------------YELSNLSQEDCW 451

Query: 444 RLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQEK- 503
            LF + AF +++E+ NP L  I  ++++K  GVPLA K LG +L  K     W ++++  
Sbjct: 452 LLFMQRAFGHQEEI-NPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWEHVRDSP 511

Query: 504 LSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQLWMANGFLHSP 563
           +    + E+ IL  LR+ Y Q+P  LK CF +C+    D  +    +I LWMA+GFL S 
Sbjct: 512 IWNLPQDESSILPALRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLLS- 571

Query: 564 EEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGN 623
            + N  +E++G++ + EL+ R F QEIE      + K+H LI  LA  +    +  S   
Sbjct: 572 -KGNMELEDVGDEVWKELYLRSFFQEIEVKDGKTYFKMHDLIHDLATSLFSANTSSSNIR 601

Query: 624 N-HPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLRLLYLSNADLQEIPNSIGTLKY 683
             + +  T + SI F E         L EK I     LR+L L ++   ++P+SIG L +
Sbjct: 632 EINKHSYTHMMSIGFAEVVFFYTLPPL-EKFI----SLRVLNLGDSTFNKLPSSIGDLVH 601

Query: 684 LRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYL 730
           LRYL+L+G + ++ LP  +C LQ+LQTL L  C+ L  LPK+   L SLR L
Sbjct: 692 LRYLNLYG-SGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNL 601

BLAST of Chy4G084330 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 217.6 bits (553), Expect = 5.7e-55
Identity = 187/740 (25.27%), Postives = 326/740 (44.05%), Query Frame = 0

Query: 24  KRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEV--FKQKDI 83
           K++    S ++AVL DA+EKQ + + ++NWL +L    Y+V++ +D+ K E   FKQ  +
Sbjct: 32  KKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDCKTEAARFKQAVL 91

Query: 84  GKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETTHTF 143
           G+      +   ++         + K+  ++L+ IA     FHL  + I+  +      F
Sbjct: 92  GRYHPRTITFCYKVGK-------RMKEMMEKLDAIAEERRNFHLDERIIERQAARRQTGF 151

Query: 144 PSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILND--FNKCAHVFHIVGEAGIGKST 203
                               +    ++ D I   ++N+  +++   V  I+G  G+GK+T
Sbjct: 152 VLTEP--------------KVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGKTT 211

Query: 204 VARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQLLSKVKR 263
           +A+ ++N   +  +   + WVCV + F+  RL+K +    + K +  D+    L  K++ 
Sbjct: 212 LAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSL-GDMDLAPLQKKLQE 271

Query: 264 LLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQKT 323
           LL  + + +V  D  + N D      L  +LK+G  G+ I++TT+ E+I +         
Sbjct: 272 LLNGKRYFLVLDD--VWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGS--------- 331

Query: 324 ERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNLKKNPVAG 383
                                                                    + G
Sbjct: 332 ---------------------------------------------------------IMG 391

Query: 384 DRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQSSS 443
             +                                            +++L  LS++   
Sbjct: 392 TLQ--------------------------------------------LYQLSNLSQEDCW 451

Query: 444 RLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQE-K 503
            LFK+ AF ++ E  +P+L +I  ++++KC GVPLA K LG LL  K   +EW ++++ +
Sbjct: 452 LLFKQRAFCHQTET-SPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRDSE 511

Query: 504 LSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQLWMANGFLHSP 563
           +    + EN +L  LR+ Y  +P  L+ CF +C+    D  I    +I LWMA+ FL S 
Sbjct: 512 IWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLLS- 571

Query: 564 EEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKI-TQEQSEGSGG 623
            + N  +E++G + + EL+ R F QEIE      + K+H LI  LA  + +   S  S  
Sbjct: 572 -KGNMELEDVGNEVWNELYLRSFFQEIEVKSGKTYFKMHDLIHDLATSMFSASASSRSIR 631

Query: 624 NNHPNEVTEIRSIAFQERNMVLPN-----ASLTEKCIWTYKGLRLLYLSNADLQEIPNSI 683
             +  +  ++  I    ++M+        +S +      +  LR+L LSN++ +++P+S+
Sbjct: 632 QINVKDDEDMMFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSV 633

Query: 684 GTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVT 743
           G L +LRYLDL GN KI  LP  +C LQ+LQTL L +C +L  LPK    L SLR L + 
Sbjct: 692 GDLVHLRYLDLSGN-KICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLD 633

Query: 744 TNKLRLDKNGVGTMNSLRFL 753
              L      +G +  L+ L
Sbjct: 752 HCPLTSMPPRIGLLTCLKTL 633

BLAST of Chy4G084330 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 4.8e-54
Identity = 192/739 (25.98%), Postives = 323/739 (43.71%), Query Frame = 0

Query: 24  KRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEV--FKQKDI 83
           +++    S+++AVL DA+EKQ +++ + NWL +L +  Y+V++ + E K E   F+Q  +
Sbjct: 32  EKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEAIRFEQSRL 91

Query: 84  GKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETTHTF 143
           G       +  ++I         + K+  ++L+ I+    +FH   K  +  +   T   
Sbjct: 92  GFYHPGIINFRHKIGR-------RMKEIMEKLDAISEERRKFHFLEKITERQAAAATR-- 151

Query: 144 PSASEISIRHLKPSWQLRYP-LTDASRQYDNIYDGILNDFNKCAH--VFHIVGEAGIGKS 203
                      +  + L  P +    ++ D I   ++N+ N      VF I+G  G+GK+
Sbjct: 152 -----------ETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEELPVFPIIGMGGLGKT 211

Query: 204 TVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHAD-NKEICEDLTTEQLLSKV 263
           T+A+ ++N   V  +   + WVCV + F+  RL+K +  + + +    EDL + Q   K+
Sbjct: 212 TLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQ--KKL 271

Query: 264 KRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQ 323
           + LL  + +L+V  D  + N D      L  +L +G  G+ I+ TT+ E++ +       
Sbjct: 272 QELLNGKRYLLVLDD--VWNDDLEKWAKLRAVLTVGARGASILATTRLEKVGS------- 331

Query: 324 KTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNLKKNPV 383
                                                                      +
Sbjct: 332 -----------------------------------------------------------I 391

Query: 384 AGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQS 443
            G  +  H                                            L  LS   
Sbjct: 392 MGTLQPYH--------------------------------------------LSNLSPHD 451

Query: 444 SSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQE 503
           S  LF + AF  ++E  NP L  I  ++++KC GVPLA K LG LL  K   +EW ++++
Sbjct: 452 SLLLFMQRAFGQQKEA-NPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREESEWEHVRD 511

Query: 504 -KLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQLWMANGFLH 563
            ++    + E+ IL  LR+ Y  +P  L+ CF +C+    D  +   ++I LWMA+GFL 
Sbjct: 512 NEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLL 571

Query: 564 SPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSG 623
           S  + N  +E++G + + EL+ R F QEIE      + K+H LI  LA  +    +  S 
Sbjct: 572 S--KGNLELEDVGNEVWNELYLRSFFQEIEAKSGNTYFKIHDLIHDLATSLF--SASASC 625

Query: 624 GNNHPNEVTEIR---SIAFQERNMVLPNASLTEKCIWTYKGLRLLYLSNADLQEIPNSIG 683
           GN     V + +   SI F         +S +   +  +  LR+L LS + L+++P+SIG
Sbjct: 632 GNIREINVKDYKHTVSIGF-----AAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIG 625

Query: 684 TLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTT 743
            L +LRYLDL  N   + LP  +C LQ+LQTL + +C +L  LPK    L SLR+L V  
Sbjct: 692 DLLHLRYLDLSCN-NFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDG 625

Query: 744 NKLRLDKNGVGTMNSLRFL 753
             L      +G +  L+ L
Sbjct: 752 CPLTSTPPRIGLLTCLKTL 625

BLAST of Chy4G084330 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 1.2e-44
Identity = 193/781 (24.71%), Postives = 321/781 (41.10%), Query Frame = 0

Query: 24  KRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEVFKQKDIG- 83
           +R+   + ++ AVL+DAEEKQ  N  +  W+ EL++V Y  E+++D+   E  +  +IG 
Sbjct: 40  ERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALR-LNIGA 99

Query: 84  --------KQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTS 143
                   +Q+    S  + +  N    + + ++    L ++A++     LK    + T+
Sbjct: 100 ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLK----ELTA 159

Query: 144 METTHTFPSASEISIRHLKPSWQLRYPLTDASRQY--DNIYDGIL------NDFNKCAHV 203
           M      P+ S                L D S  +  D+  D I+      N  +    V
Sbjct: 160 MIPKQRLPTTS----------------LVDESEVFGRDDDKDEIMRFLIPENGKDNGITV 219

Query: 204 FHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICE 263
             IVG  G+GK+T+++ LYN   V     ++ W  V E F+  ++ K+VY    ++  CE
Sbjct: 220 VAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRP-CE 279

Query: 264 DLTTEQLLSKVKRLLKVE--TFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQ 323
               + L  K+K  L      FL+V  DL   N  D    +L Q       GS+I+VTT+
Sbjct: 280 FTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWD--LLRQPFIHAAQGSQILVTTR 339

Query: 324 TEEIANDYEDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANK 383
           ++ +A+                                                      
Sbjct: 340 SQRVAS------------------------------------------------------ 399

Query: 384 IYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVC 443
                                                                     +C
Sbjct: 400 ---------------------------------------------------------IMC 459

Query: 444 QI-IFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLL 503
            + +  L+ LS      LF +  F N++   N E+  + ++++ KC G+PLA+K LG +L
Sbjct: 460 AVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVL 519

Query: 504 SSKTSIAEWTNIQEK--LSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIIS 563
             +  + EW  +       L   K N +L VLRV Y  +P+ LK CF +CS         
Sbjct: 520 RFEGKVIEWERVLSSRIWDLPADKSN-LLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFE 579

Query: 564 SNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQ 623
            + ++ LWMA GFL      +  +E +G +YF EL SR  +Q+ +   +     +H  I 
Sbjct: 580 KDKVVLLWMAEGFLQQ-TRSSKNLEELGNEYFSELESRSLLQKTKTRYI-----MHDFIN 639

Query: 624 KLAHKITQE-QSEGSGG----------------NNH--PNEVTEIRSIAFQER--NMVLP 683
           +LA   + E  S+   G                +N+  P E   +R + F      + L 
Sbjct: 640 ELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLT 677

Query: 684 NAS--------LTEKCIWTYKGLRLLYLSNADLQEI-PNSIGTLKYLRYLDLHGNTKIKH 743
           N+S        ++EK + T   LR+L LS+  +  + P+    + + R+LDL   T+++ 
Sbjct: 700 NSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRTELEK 677

Query: 744 LPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRF 753
           LP S+C + +LQTL+L  CS+L++LP DI NLI+LRYL +   KLR      G + SL+ 
Sbjct: 760 LPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQT 677

BLAST of Chy4G084330 vs. ExPASy TrEMBL
Match: A0A0A0LBH9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G815400 PE=4 SV=1)

HSP 1 Score: 1617.8 bits (4188), Expect = 0.0e+00
Identity = 819/909 (90.10%), Postives = 841/909 (92.52%), Query Frame = 0

Query: 1   MAHCIYTQAENILTKLKYCPPYQKRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNV 60
           MAHCIYTQAENILTKLK  PPYQKRIEY MSSLKAVLLDAEEKQEQNQ L+NWL ELQNV
Sbjct: 1   MAHCIYTQAENILTKLKDSPPYQKRIEYAMSSLKAVLLDAEEKQEQNQRLQNWLKELQNV 60

Query: 61  FYQVENSIDEFKWEVFKQKDIGKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRM 120
           FYQVE+SIDEFKWE+FKQKDIGKQVLA FSCSN+ISANKLKQKCKRKQ CDELN IATRM
Sbjct: 61  FYQVEDSIDEFKWEIFKQKDIGKQVLAPFSCSNQISANKLKQKCKRKQVCDELNNIATRM 120

Query: 121 YEFHLKVKHIDSTSMETTHTFPSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILNDF 180
           YEFHLKVKHIDS SMETTHTFPSASEISIRHLKPSWQL YPL DASR YD IYDGILN F
Sbjct: 121 YEFHLKVKHIDSISMETTHTFPSASEISIRHLKPSWQLLYPLIDASRTYDEIYDGILNVF 180

Query: 181 NKCAHVFHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHAD 240
           N+C HVFHIVGEAGIGKSTVARFLYNHN+VVG  TSRYWVCVEEGFN HRLVKEVYSHAD
Sbjct: 181 NECTHVFHIVGEAGIGKSTVARFLYNHNNVVGKYTSRYWVCVEEGFNKHRLVKEVYSHAD 240

Query: 241 NKEICEDLTTEQLLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKII 300
           NKEICEDLTTEQLLSKVKRLLKVETFL+VFQDLSITNL DRS  VL +LL+MGQHGSKII
Sbjct: 241 NKEICEDLTTEQLLSKVKRLLKVETFLLVFQDLSITNLKDRSPLVLNELLEMGQHGSKII 300

Query: 301 VTTQTEEIANDYEDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADA 360
           VTTQTEEIAN  +D G KTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQ EETADA
Sbjct: 301 VTTQTEEIANYLQDRGYKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQIEETADA 360

Query: 361 IANKIYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAV---FNPVIPPR 420
           IANKIY                 EHS SLTEDAQNL VPIP+VTEDT +    NP IPP 
Sbjct: 361 IANKIY-----------------EHSLSLTEDAQNLEVPIPQVTEDTNIGLGINPDIPPI 420

Query: 421 KQDDTVCQIIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK 480
           KQDDT  Q IFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK
Sbjct: 421 KQDDTEYQTIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK 480

Query: 481 CLGSLLSSKTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSND 540
           CLGSLLSS+TSIA+W NI+EKLSLQEKKENGIL+VLRVCYDQMPSQLKPCFLHCSQL ND
Sbjct: 481 CLGSLLSSETSIAKWKNIEEKLSLQEKKENGILHVLRVCYDQMPSQLKPCFLHCSQLPND 540

Query: 541 RIISSNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLH 600
           RIISSNDMIQLWMANG LHSPEEKNSTMENIGEKYFMELWSR FIQEIEEHGLGYWIKLH
Sbjct: 541 RIISSNDMIQLWMANGLLHSPEEKNSTMENIGEKYFMELWSRYFIQEIEEHGLGYWIKLH 600

Query: 601 PLIQKLAHKITQEQSEGSGGNNHPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLRL 660
           PLIQKLAHKITQEQSEGSGGNNHP EVTEIRSIAFQERNMVLPNASLTEKCIW YKGLRL
Sbjct: 601 PLIQKLAHKITQEQSEGSGGNNHPKEVTEIRSIAFQERNMVLPNASLTEKCIWKYKGLRL 660

Query: 661 LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP 720
           LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP
Sbjct: 661 LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP 720

Query: 721 KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERQNCLAGLETLM 780
           KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFER +CLAGLETLM
Sbjct: 721 KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERPDCLAGLETLM 780

Query: 781 IYNCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWL 840
           IYNCTTLKLLPD+MRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWL
Sbjct: 781 IYNCTTLKLLPDEMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWL 840

Query: 841 ENSAETLRILQIINCPIRIMERQGIENYESLENTIIYGAVRFETTTPGYDFEHRNLAMRN 900
           ENSAETLR LQIINCPIRIMERQGIE YE++ENTIIYGAVRFE   PGYDFEHRNLA+RN
Sbjct: 841 ENSAETLRTLQIINCPIRIMERQGIEKYEAVENTIIYGAVRFEMAPPGYDFEHRNLAVRN 892

Query: 901 GNEEMYIYP 907
           GNEEM+IYP
Sbjct: 901 GNEEMHIYP 892

BLAST of Chy4G084330 vs. ExPASy TrEMBL
Match: A0A1S3B6X8 (putative disease resistance protein RGA1 OS=Cucumis melo OX=3656 GN=LOC103486832 PE=4 SV=1)

HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 729/901 (80.91%), Postives = 780/901 (86.57%), Query Frame = 0

Query: 1   MAHCIYTQAENILTKLKYCPPYQKRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNV 60
           MA+CIYTQAENILTKLK CP YQKRIEYTMSSLKA+LLDAEEKQEQNQ L +WL ELQNV
Sbjct: 1   MAYCIYTQAENILTKLKDCPAYQKRIEYTMSSLKAILLDAEEKQEQNQRLHDWLKELQNV 60

Query: 61  FYQVENSIDEFKWEVFKQKDIGKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRM 120
           FYQVE+SIDEFKWE FKQKD GKQVLAHFSCS+RISANKLKQ  KR + CDELNKIA  M
Sbjct: 61  FYQVEDSIDEFKWEFFKQKDTGKQVLAHFSCSSRISANKLKQNFKRNKVCDELNKIAANM 120

Query: 121 YEFHLKVKHIDSTSMETTHTFPSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILNDF 180
           YEFHLKVKHI S SMETTH+FPSASEIS RHLKPSWQL YPL  ASR YD  YDGILN F
Sbjct: 121 YEFHLKVKHIGSISMETTHSFPSASEISTRHLKPSWQLLYPLKHASRNYDEAYDGILNVF 180

Query: 181 NKCAH-VFHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHA 240
           NK  H  FHIVGEAGIGKST+ARFLYNH +VV   T RYWVCVEEGFNTHRL+KE+YSHA
Sbjct: 181 NKYTHGFFHIVGEAGIGKSTMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHA 240

Query: 241 DNKEICEDLTTEQLLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKI 300
           DNKE  EDLTTEQLLSK  RL + ETFL+VFQDLSITNLD  S+ VL +LL+MG H SKI
Sbjct: 241 DNKETREDLTTEQLLSKFIRLQREETFLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKI 300

Query: 301 IVTTQTEEIANDYEDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETAD 360
           IVTTQTEEIA D + HG KTE +SK+NLANGD VSA  QSLTKNTQNQAVPGSQTEETA 
Sbjct: 301 IVTTQTEEIAKDIDVHGYKTEIQSKKNLANGDEVSADKQSLTKNTQNQAVPGSQTEETAG 360

Query: 361 AIANKIYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQ 420
           AIAN IYE E+NL+KNPVAGD ESE SR +T+DAQNL V IP+VTE             +
Sbjct: 361 AIANMIYETEKNLEKNPVAGDGESESSRLITKDAQNLDVSIPQVTE------------CE 420

Query: 421 DDTVCQIIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCL 480
            D   Q IFKL+KLSKQSSS LFKEYAFRN  EV+NPELTKIVDQLLEKCMGVPLAIKCL
Sbjct: 421 PDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCL 480

Query: 481 GSLLSSKTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRI 540
           GSLLSSKTSIAEW NI++KL  QEKKE+GIL+VLRVCYDQMPSQLKPCFL+CSQL NDRI
Sbjct: 481 GSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRI 540

Query: 541 ISSNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPL 600
            SSNDMIQLWMANG LHSPEEKN  MENIG+KYFMELWSRCFIQEIEEHGLGYWIKLHPL
Sbjct: 541 FSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPL 600

Query: 601 IQKLAHKITQEQSEGSGGNNHPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLRLLY 660
           IQKLAHKITQEQSEGS GNNH  E+TEIRSIAFQERNMVLPNASLTEKCIW YK LRLLY
Sbjct: 601 IQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLY 660

Query: 661 LSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKD 720
           L NADLQEIPNSIGTLKYLRYLDLHGN KIKHLP+SIC+LQSLQTLIL SCSALEDLPKD
Sbjct: 661 LGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKD 720

Query: 721 IRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERQNCLAGLETLMIY 780
           IRNLISLRYLWVTTNKL L KNGVGTM SLRFLAIGGC NLENLFER +CLAGLETLMIY
Sbjct: 721 IRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIY 780

Query: 781 NCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWLEN 840
           NC TLKLLP++MRYLKSLQNLMIW CKQLTLNL+EVEFKLQRFT+KELP+VE LP+WLEN
Sbjct: 781 NCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQRFTVKELPKVESLPRWLEN 840

Query: 841 SAETLRILQIINCPIRIMERQGIENYESLENTIIYGAVRFETTTPGYDFEHRNLAMRNGN 900
           S ETLR LQIINCPIR+ME    + Y +LE  +I+G+VRF+ T P YDFEHRNL +  GN
Sbjct: 841 SIETLRTLQIINCPIRLMELPVDKKYRALEIFLIHGSVRFD-TMPDYDFEHRNLTIFRGN 888

BLAST of Chy4G084330 vs. ExPASy TrEMBL
Match: A0A5A7UFA9 (Putative disease resistance protein RGA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006100 PE=4 SV=1)

HSP 1 Score: 1368.6 bits (3541), Expect = 0.0e+00
Identity = 703/872 (80.62%), Postives = 753/872 (86.35%), Query Frame = 0

Query: 30  MSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEVFKQKDIGKQVLAHF 89
           MSSLKA+LLDAEEKQEQNQ L +WL ELQNVFYQVE+SIDEFKWE FKQKD GKQVLAHF
Sbjct: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60

Query: 90  SCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETTHTFPSASEISI 149
           SCS+RISANKLKQ  KR + CDELNKIA  MYEFHLKVKHI S SMETTH+FPSASEIS 
Sbjct: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120

Query: 150 RHLKPSWQLRYPLTDASRQYDNIYDGILNDFNKCAH-VFHIVGEAGIGKSTVARFLYNHN 209
           RHLKPSWQL YPL  ASR YD  YDGILN FNK  H  FHIVGEAGIGKST+ARFLYNH 
Sbjct: 121 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 180

Query: 210 DVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLLKVETFLI 269
           +VV   T RYWVCVEEGFNTHRL+KE+YSHADNKE  EDLTTEQLLSK  RL + ETFL+
Sbjct: 181 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 240

Query: 270 VFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQKTERRSKENLA 329
           VFQDLSITNLD  S+ VL +LL+MG H SKIIVTTQTEEIA D + HG KTE +SK+NLA
Sbjct: 241 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 300

Query: 330 NGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNLKKNPVAGDRESEHSRS 389
           NGD VSA  QSLTKNTQNQAVPGSQTEETA AIAN IYE E+NL+KNPVAGD ESE SR 
Sbjct: 301 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 360

Query: 390 LTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQSSSRLFKEYAFR 449
           +T+DAQNL V IP+VTE             + D   Q IFKL+KLSKQSSS LFKEYAFR
Sbjct: 361 ITKDAQNLDVSIPQVTE------------CEPDRGYQTIFKLKKLSKQSSSLLFKEYAFR 420

Query: 450 NRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQEKLSLQEKKENG 509
           N  EV+NPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEW NI++KL  QEKKE+G
Sbjct: 421 NSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDG 480

Query: 510 ILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQLWMANGFLHSPEEKNSTMENI 569
           IL+VLRVCYDQMPSQLKPCFL+CSQL NDRI SSNDMIQLWMANG LHSPEEKN  MENI
Sbjct: 481 ILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENI 540

Query: 570 GEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPNEVTEIR 629
           G+KYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGS GNNH  E+TEIR
Sbjct: 541 GKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIR 600

Query: 630 SIAFQERNMVLPNASLTEKCIWTYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHGNTK 689
           SIAFQERNMVLPNASLTEKCIW YK LRLLYL NADLQEIPNSIGTLKYLRYLDLHGN K
Sbjct: 601 SIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQK 660

Query: 690 IKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNS 749
           IKHLP+SIC+LQSLQTLIL SCSALEDLPKDIRNLISLRYLWVTTNKL L KNGVGTM S
Sbjct: 661 IKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTS 720

Query: 750 LRFLAIGGCNNLENLFERQNCLAGLETLMIYNCTTLKLLPDKMRYLKSLQNLMIWSCKQL 809
           LRFLAIGGC NLENLFER +CLAGLETLMIYNC TLKLLP++MRYLKSLQNLMIW CKQL
Sbjct: 721 LRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQL 780

Query: 810 TLNLKEVEFKLQRFTIKELPRVERLPQWLENSAETLRILQIINCPIRIMERQGIENYESL 869
           TLNL+EVEFKLQRFT+KELP+VE LP+WLENS ETLR LQIINCPIR+ME    + Y +L
Sbjct: 781 TLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRAL 840

Query: 870 ENTIIYGAVRFETTTPGYDFEHRNLAMRNGNE 901
           E  +I+G+VRF+ T P YDFEHRNL +  GNE
Sbjct: 841 EIFLIHGSVRFD-TMPDYDFEHRNLTIFRGNE 859

BLAST of Chy4G084330 vs. ExPASy TrEMBL
Match: A0A6J1J5Q0 (putative disease resistance protein RGA1 OS=Cucurbita maxima OX=3661 GN=LOC111481987 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.9e-258
Identity = 489/875 (55.89%), Postives = 604/875 (69.03%), Query Frame = 0

Query: 30  MSSLKAVLLDAEEKQEQNQH--LRNWLMELQNVFYQVENSIDEFKWEVFKQKDIGKQVLA 89
           M SLK++L DAEEKQEQ Q+  LRNWL ELQN+FYQ+E+S+DEFKW+ F+Q   GK+V A
Sbjct: 1   MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 60

Query: 90  HFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTS-METTHTFPSASE 149
            F CS+ ISA+K K   K K+ C+ LN +A RMYEFHL  KH DS   M+T H+F S +E
Sbjct: 61  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 120

Query: 150 ISIRHLKPSWQLRYPLTDASRQY-DNIYDGILNDFNKCAHVFHIVGEAGIGKSTVARFLY 209
           +S+RH+KPSWQL+Y L DA   Y DN Y  +L  F      F IVGEAGIGKST+A+FLY
Sbjct: 121 VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY 180

Query: 210 NHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLLKVET 269
           +H  VV    SR+WVCV+EGF+T RL++EVYS    KEIC DL+ +QL  KV++LLK  +
Sbjct: 181 HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS 240

Query: 270 FLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQKTERRSKE 329
           FL+V QDLS TNLDD S+  L +LL MG   SKI+VTT                      
Sbjct: 241 FLLVIQDLSTTNLDDSSA--LMELLDMGNEDSKILVTT---------------------- 300

Query: 330 NLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKI-YEIEQNLKKNPVAGDRESE 389
                                      Q+EETADA+  +I Y  E+   +N  + D+  E
Sbjct: 301 ---------------------------QSEETADALGVRIVYAFEKQSMQN--SNDKNQE 360

Query: 390 HSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQSSSRLFKE 449
                        VP                            FKLE+LS+  S++LFK 
Sbjct: 361 -------------VPTG--------------------------FKLERLSEDKSAKLFKR 420

Query: 450 YAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQEKLSLQEK 509
           YAFR+RQEV     T+I +Q+L+KC GVPLAIKC+GSLLSSK  IAEW NI++KL  QE+
Sbjct: 421 YAFRDRQEVNREVFTEIENQILQKCKGVPLAIKCMGSLLSSKIRIAEWKNIKDKLWAQEE 480

Query: 510 KENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQLWMANGFLHSPEEKNST 569
           + +GIL++LR+CY+QMPS LKPCFLHC+QL  DRI+SSND+IQLWMA+G LHSPEE +++
Sbjct: 481 ENDGILHILRLCYNQMPSHLKPCFLHCAQLP-DRILSSNDVIQLWMAHGLLHSPEENHAS 540

Query: 570 MENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPNEV 629
           M+NIGE+YFMELWSRCF+QEIEEHGLGYW KLHPLIQKLAH + QEQSEG  G      V
Sbjct: 541 MKNIGERYFMELWSRCFVQEIEEHGLGYWFKLHPLIQKLAHVLAQEQSEGLVGT---KPV 600

Query: 630 TEIRSIAFQERNMVLPNASLTEKCIWTYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLH 689
           T+I+S+AF  R+MV PNASL   CI  Y  LRLL+L NA+++EIPN+IGTL  LRYLDLH
Sbjct: 601 TQIKSLAFHVRDMVPPNASLPGMCIPKYNSLRLLHLGNANIEEIPNAIGTLTSLRYLDLH 660

Query: 690 GNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVG 749
           GN  IK LPNSICNLQSLQTLIL SCS L++LP DIRNLISL+YLW+TT +LRL KNGVG
Sbjct: 661 GNKNIKRLPNSICNLQSLQTLILESCSELQELPNDIRNLISLKYLWITTKELRLHKNGVG 720

Query: 750 TMNSLRFLAIGGCNNLENLFERQNCLAGLETLMIYNCTTLKLLPDKMRYLKSLQNLMIWS 809
           TM SL+FLAIGGC NL+ LFER  CLA LETLMIYNC TLK LP++M YLKSLQNL+IW 
Sbjct: 721 TMTSLQFLAIGGCKNLQILFERPECLADLETLMIYNCDTLKSLPNEMIYLKSLQNLVIWG 778

Query: 810 CKQLTLNLKEVEFKLQRFTIKELPRVERLPQWLENSAETLRILQIINCPIRIMERQGIEN 869
           CKQLTL L+  EF+LQRFTI+ELP+VE LP+WL+ S  TLR LQIINCPI I   + ++ 
Sbjct: 781 CKQLTLTLENAEFRLQRFTIRELPKVEELPEWLDGSIGTLRNLQIINCPITINVGR-LQA 778

Query: 870 YESLENTIIYGAVRFETTTPGYDFEHRNLAMRNGN 900
           ++ +E   I G+V+ +   PGY F++RN  +R  +
Sbjct: 841 FKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSS 778

BLAST of Chy4G084330 vs. ExPASy TrEMBL
Match: A0A6J1DLK5 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111021565 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 7.0e-258
Identity = 499/855 (58.36%), Postives = 606/855 (70.88%), Query Frame = 0

Query: 1   MAHCIYTQAENILTKLKYCPPYQKRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNV 60
           MAH IY++AENIL KLK CP   +R+EYTM SLKA+LLDAEEKQEQN  LRNWL ELQ+V
Sbjct: 41  MAHFIYSRAENILNKLKDCPIDLRRMEYTMLSLKAILLDAEEKQEQNHGLRNWLKELQDV 100

Query: 61  FYQVENSIDEFKWEVFKQKDIGKQVLAHFSCSNRISAN-KLKQKCKRKQACDELNKIATR 120
           FYQ E+SIDE KWE   Q+ IG++V A FSCS++ +A+ K     K K+ CD L++IA++
Sbjct: 101 FYQTEDSIDELKWE---QEGIGRKVRARFSCSDKTAAHKKTNPTSKMKKLCDHLDEIASK 160

Query: 121 MYEFHLKVKHIDSTSMETTHTFPSASEISIRHLKPSWQLRYPLTDASRQYDNI-YDGILN 180
           MYEFHL  +H  S  MET H+F SA+E+S RH++PSWQL YPLTDA   Y N  Y  +LN
Sbjct: 161 MYEFHLTEEHTSSIRMETAHSFLSATEVSTRHMRPSWQLFYPLTDAPNSYCNSRYVELLN 220

Query: 181 DF-NKCAHVFHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYS 240
           DF +     FHIVGEAGIGKST+A+F Y+H+DV  + TSR WVCV++G+NT RL+KEVYS
Sbjct: 221 DFIHSKQQFFHIVGEAGIGKSTLAKFFYSHHDVCSSFTSRSWVCVKDGYNTKRLLKEVYS 280

Query: 241 HADNKEICEDLTTEQLLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGS 300
             D+KE+ +D T +QLL KV+R L+ +TFL+VFQDLS  NL+D  S +L  LL++G +GS
Sbjct: 281 FQDDKEVRDDFTKKQLLLKVQRFLRGQTFLVVFQDLSSKNLEDDRS-MLKNLLELGNYGS 340

Query: 301 KIIVTTQTEEIANDYE-DHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEE 360
           KIIVTTQ EEIA+D +     KT++ S++N  N     A N     + QNQ         
Sbjct: 341 KIIVTTQNEEIADDLKLKKVYKTKKLSEQNQVNTIIHQAINIVEGLSEQNQ--------- 400

Query: 361 TADAIANKIYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPP 420
                 N I                  EH                               
Sbjct: 401 -----VNTI------------------EH------------------------------- 460

Query: 421 RKQDDTVCQIIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAI 480
                   Q I  +E LS+Q+S  LFK+YAFR+ +E +N EL  I  Q++EKC   PLAI
Sbjct: 461 --------QAIIIVEGLSEQNSFSLFKDYAFRDTKEAQNLEL--IAKQIVEKCKRFPLAI 520

Query: 481 KCLGSLLSSKTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSN 540
           KC+GSLLSSK  IAEW  +  KL  QE+++  IL +LRVCY+ MPS LKPCFL  SQL +
Sbjct: 521 KCVGSLLSSKNRIAEWNEVNNKLCAQEEEDGSILPILRVCYNLMPSYLKPCFLLYSQLPD 580

Query: 541 DRIISSNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKL 600
           DRI+SSND+IQLWMA+G LHSP+E + ++EN GEKYFMELWSRCF+QE+EEHGLG W KL
Sbjct: 581 DRILSSNDVIQLWMAHGLLHSPKENSLSLENTGEKYFMELWSRCFVQEMEEHGLGCWFKL 640

Query: 601 HPLIQKLAHKITQEQSEGSGGNNHPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLR 660
           HPLI+KLAH +T+EQ+EG  G   P+  TEIRSIAF+ R+ V P+  L  KCI  +K LR
Sbjct: 641 HPLIRKLAHVLTREQTEGLVGTK-PD--TEIRSIAFRVRDKVKPSVFLANKCISMFKCLR 700

Query: 661 LLYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDL 720
           LLYL NADLQEIPN+IGTLKYLRYLDL GN +IK LPNSIC LQSLQTLIL SCS+L +L
Sbjct: 701 LLYLGNADLQEIPNAIGTLKYLRYLDLQGNERIKQLPNSICKLQSLQTLILDSCSSLVEL 760

Query: 721 PKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERQNCLAGLETL 780
           P DI NLISLRYLWVT+NKLRL KNGVGTM SLRFLAIGGC+NL+ LFER +CLAGLETL
Sbjct: 761 PNDIANLISLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDNLQILFERPDCLAGLETL 814

Query: 781 MIYNCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQW 840
           MIYNC  LK LP++MRYLKSLQNL+IW CKQL L LKEVEFKLQRFTI+ELP+VE LPQW
Sbjct: 821 MIYNCNALKSLPNEMRYLKSLQNLVIWGCKQLKLTLKEVEFKLQRFTIRELPKVEELPQW 814

Query: 841 LENSAETLRILQIIN 852
           L+ S+ETLR  Q +N
Sbjct: 881 LQGSSETLR-FQYVN 814

BLAST of Chy4G084330 vs. NCBI nr
Match: XP_004136758.3 (putative disease resistance protein RGA1 [Cucumis sativus] >KGN59375.1 hypothetical protein Csa_002172 [Cucumis sativus])

HSP 1 Score: 1616 bits (4184), Expect = 0.0
Identity = 819/909 (90.10%), Postives = 841/909 (92.52%), Query Frame = 0

Query: 1   MAHCIYTQAENILTKLKYCPPYQKRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNV 60
           MAHCIYTQAENILTKLK  PPYQKRIEY MSSLKAVLLDAEEKQEQNQ L+NWL ELQNV
Sbjct: 1   MAHCIYTQAENILTKLKDSPPYQKRIEYAMSSLKAVLLDAEEKQEQNQRLQNWLKELQNV 60

Query: 61  FYQVENSIDEFKWEVFKQKDIGKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRM 120
           FYQVE+SIDEFKWE+FKQKDIGKQVLA FSCSN+ISANKLKQKCKRKQ CDELN IATRM
Sbjct: 61  FYQVEDSIDEFKWEIFKQKDIGKQVLAPFSCSNQISANKLKQKCKRKQVCDELNNIATRM 120

Query: 121 YEFHLKVKHIDSTSMETTHTFPSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILNDF 180
           YEFHLKVKHIDS SMETTHTFPSASEISIRHLKPSWQL YPL DASR YD IYDGILN F
Sbjct: 121 YEFHLKVKHIDSISMETTHTFPSASEISIRHLKPSWQLLYPLIDASRTYDEIYDGILNVF 180

Query: 181 NKCAHVFHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHAD 240
           N+C HVFHIVGEAGIGKSTVARFLYNHN+VVG  TSRYWVCVEEGFN HRLVKEVYSHAD
Sbjct: 181 NECTHVFHIVGEAGIGKSTVARFLYNHNNVVGKYTSRYWVCVEEGFNKHRLVKEVYSHAD 240

Query: 241 NKEICEDLTTEQLLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKII 300
           NKEICEDLTTEQLLSKVKRLLKVETFL+VFQDLSITNL DRS  VL +LL+MGQHGSKII
Sbjct: 241 NKEICEDLTTEQLLSKVKRLLKVETFLLVFQDLSITNLKDRSPLVLNELLEMGQHGSKII 300

Query: 301 VTTQTEEIANDYEDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADA 360
           VTTQTEEIAN  +D G KTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQ EETADA
Sbjct: 301 VTTQTEEIANYLQDRGYKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQIEETADA 360

Query: 361 IANKIYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAV---FNPVIPPR 420
           IANKIYE                 HS SLTEDAQNL VPIP+VTEDT +    NP IPP 
Sbjct: 361 IANKIYE-----------------HSLSLTEDAQNLEVPIPQVTEDTNIGLGINPDIPPI 420

Query: 421 KQDDTVCQIIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK 480
           KQDDT  Q IFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK
Sbjct: 421 KQDDTEYQTIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK 480

Query: 481 CLGSLLSSKTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSND 540
           CLGSLLSS+TSIA+W NI+EKLSLQEKKENGIL+VLRVCYDQMPSQLKPCFLHCSQL ND
Sbjct: 481 CLGSLLSSETSIAKWKNIEEKLSLQEKKENGILHVLRVCYDQMPSQLKPCFLHCSQLPND 540

Query: 541 RIISSNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLH 600
           RIISSNDMIQLWMANG LHSPEEKNSTMENIGEKYFMELWSR FIQEIEEHGLGYWIKLH
Sbjct: 541 RIISSNDMIQLWMANGLLHSPEEKNSTMENIGEKYFMELWSRYFIQEIEEHGLGYWIKLH 600

Query: 601 PLIQKLAHKITQEQSEGSGGNNHPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLRL 660
           PLIQKLAHKITQEQSEGSGGNNHP EVTEIRSIAFQERNMVLPNASLTEKCIW YKGLRL
Sbjct: 601 PLIQKLAHKITQEQSEGSGGNNHPKEVTEIRSIAFQERNMVLPNASLTEKCIWKYKGLRL 660

Query: 661 LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP 720
           LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP
Sbjct: 661 LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP 720

Query: 721 KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERQNCLAGLETLM 780
           KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFER +CLAGLETLM
Sbjct: 721 KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERPDCLAGLETLM 780

Query: 781 IYNCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWL 840
           IYNCTTLKLLPD+MRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWL
Sbjct: 781 IYNCTTLKLLPDEMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWL 840

Query: 841 ENSAETLRILQIINCPIRIMERQGIENYESLENTIIYGAVRFETTTPGYDFEHRNLAMRN 900
           ENSAETLR LQIINCPIRIMERQGIE YE++ENTIIYGAVRFE   PGYDFEHRNLA+RN
Sbjct: 841 ENSAETLRTLQIINCPIRIMERQGIEKYEAVENTIIYGAVRFEMAPPGYDFEHRNLAVRN 892

Query: 901 GNEEMYIYP 906
           GNEEM+IYP
Sbjct: 901 GNEEMHIYP 892

BLAST of Chy4G084330 vs. NCBI nr
Match: XP_008443163.1 (PREDICTED: putative disease resistance protein RGA1 [Cucumis melo])

HSP 1 Score: 1419 bits (3674), Expect = 0.0
Identity = 730/906 (80.57%), Postives = 781/906 (86.20%), Query Frame = 0

Query: 1   MAHCIYTQAENILTKLKYCPPYQKRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNV 60
           MA+CIYTQAENILTKLK CP YQKRIEYTMSSLKA+LLDAEEKQEQNQ L +WL ELQNV
Sbjct: 1   MAYCIYTQAENILTKLKDCPAYQKRIEYTMSSLKAILLDAEEKQEQNQRLHDWLKELQNV 60

Query: 61  FYQVENSIDEFKWEVFKQKDIGKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRM 120
           FYQVE+SIDEFKWE FKQKD GKQVLAHFSCS+RISANKLKQ  KR + CDELNKIA  M
Sbjct: 61  FYQVEDSIDEFKWEFFKQKDTGKQVLAHFSCSSRISANKLKQNFKRNKVCDELNKIAANM 120

Query: 121 YEFHLKVKHIDSTSMETTHTFPSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILNDF 180
           YEFHLKVKHI S SMETTH+FPSASEIS RHLKPSWQL YPL  ASR YD  YDGILN F
Sbjct: 121 YEFHLKVKHIGSISMETTHSFPSASEISTRHLKPSWQLLYPLKHASRNYDEAYDGILNVF 180

Query: 181 NKCAH-VFHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHA 240
           NK  H  FHIVGEAGIGKST+ARFLYNH +VV   T RYWVCVEEGFNTHRL+KE+YSHA
Sbjct: 181 NKYTHGFFHIVGEAGIGKSTMARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHA 240

Query: 241 DNKEICEDLTTEQLLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKI 300
           DNKE  EDLTTEQLLSK  RL + ETFL+VFQDLSITNLD  S+ VL +LL+MG H SKI
Sbjct: 241 DNKETREDLTTEQLLSKFIRLQREETFLLVFQDLSITNLDKCSTSVLIKLLEMGHHDSKI 300

Query: 301 IVTTQTEEIANDYEDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETAD 360
           IVTTQTEEIA D + HG KTE +SK+NLANGD VSA  QSLTKNTQNQAVPGSQTEETA 
Sbjct: 301 IVTTQTEEIAKDIDVHGYKTEIQSKKNLANGDEVSADKQSLTKNTQNQAVPGSQTEETAG 360

Query: 361 AIANKIYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQ 420
           AIAN IYE E+NL+KNPVAGD ESE SR +T+DAQNL V IP+VTE             +
Sbjct: 361 AIANMIYETEKNLEKNPVAGDGESESSRLITKDAQNLDVSIPQVTEC------------E 420

Query: 421 DDTVCQIIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCL 480
            D   Q IFKL+KLSKQSSS LFKEYAFRN  EV+NPELTKIVDQLLEKCMGVPLAIKCL
Sbjct: 421 PDRGYQTIFKLKKLSKQSSSLLFKEYAFRNSPEVQNPELTKIVDQLLEKCMGVPLAIKCL 480

Query: 481 GSLLSSKTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRI 540
           GSLLSSKTSIAEW NI++KL  QEKKE+GIL+VLRVCYDQMPSQLKPCFL+CSQL NDRI
Sbjct: 481 GSLLSSKTSIAEWKNIEKKLMPQEKKEDGILHVLRVCYDQMPSQLKPCFLYCSQLPNDRI 540

Query: 541 ISSNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPL 600
            SSNDMIQLWMANG LHSPEEKN  MENIG+KYFMELWSRCFIQEIEEHGLGYWIKLHPL
Sbjct: 541 FSSNDMIQLWMANGLLHSPEEKNLAMENIGKKYFMELWSRCFIQEIEEHGLGYWIKLHPL 600

Query: 601 IQKLAHKITQEQSEGSGGNNHPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLRLLY 660
           IQKLAHKITQEQSEGS GNNH  E+TEIRSIAFQERNMVLPNASLTEKCIW YK LRLLY
Sbjct: 601 IQKLAHKITQEQSEGSWGNNHSKEITEIRSIAFQERNMVLPNASLTEKCIWRYKCLRLLY 660

Query: 661 LSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLPKD 720
           L NADLQEIPNSIGTLKYLRYLDLHGN KIKHLP+SIC+LQSLQTLIL SCSALEDLPKD
Sbjct: 661 LGNADLQEIPNSIGTLKYLRYLDLHGNQKIKHLPDSICDLQSLQTLILDSCSALEDLPKD 720

Query: 721 IRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERQNCLAGLETLMIY 780
           IRNLISLRYLWVTTNKL L KNGVGTM SLRFLAIGGC NLENLFER +CLAGLETLMIY
Sbjct: 721 IRNLISLRYLWVTTNKLHLHKNGVGTMTSLRFLAIGGCKNLENLFERPDCLAGLETLMIY 780

Query: 781 NCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWLEN 840
           NC TLKLLP++MRYLKSLQNLMIW CKQLTLNL+EVEFKLQRFT+KELP+VE LP+WLEN
Sbjct: 781 NCNTLKLLPNEMRYLKSLQNLMIWRCKQLTLNLEEVEFKLQRFTVKELPKVESLPRWLEN 840

Query: 841 SAETLRILQIINCPIRIMERQGIENYESLENTIIYGAVRFETTTPGYDFEHRNLAMRNGN 900
           S ETLR LQIINCPIR+ME    + Y +LE  +I+G+VRF+T  P YDFEHRNL +  GN
Sbjct: 841 SIETLRTLQIINCPIRLMELPVDKKYRALEIFLIHGSVRFDTM-PDYDFEHRNLTIFRGN 893

Query: 901 EEMYIY 905
           E    Y
Sbjct: 901 EMNIHY 893

BLAST of Chy4G084330 vs. NCBI nr
Match: KAA0053940.1 (putative disease resistance protein RGA1 [Cucumis melo var. makuwa] >TYK25464.1 putative disease resistance protein RGA1 [Cucumis melo var. makuwa])

HSP 1 Score: 1366 bits (3536), Expect = 0.0
Identity = 704/877 (80.27%), Postives = 754/877 (85.97%), Query Frame = 0

Query: 30  MSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEVFKQKDIGKQVLAHF 89
           MSSLKA+LLDAEEKQEQNQ L +WL ELQNVFYQVE+SIDEFKWE FKQKD GKQVLAHF
Sbjct: 1   MSSLKAILLDAEEKQEQNQRLHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGKQVLAHF 60

Query: 90  SCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETTHTFPSASEISI 149
           SCS+RISANKLKQ  KR + CDELNKIA  MYEFHLKVKHI S SMETTH+FPSASEIS 
Sbjct: 61  SCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSISMETTHSFPSASEIST 120

Query: 150 RHLKPSWQLRYPLTDASRQYDNIYDGILNDFNKCAH-VFHIVGEAGIGKSTVARFLYNHN 209
           RHLKPSWQL YPL  ASR YD  YDGILN FNK  H  FHIVGEAGIGKST+ARFLYNH 
Sbjct: 121 RHLKPSWQLLYPLKHASRNYDEAYDGILNVFNKYTHGFFHIVGEAGIGKSTMARFLYNHE 180

Query: 210 DVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLLKVETFLI 269
           +VV   T RYWVCVEEGFNTHRL+KE+YSHADNKE  EDLTTEQLLSK  RL + ETFL+
Sbjct: 181 NVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRLQREETFLL 240

Query: 270 VFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQKTERRSKENLA 329
           VFQDLSITNLD  S+ VL +LL+MG H SKIIVTTQTEEIA D + HG KTE +SK+NLA
Sbjct: 241 VFQDLSITNLDKCSTSVLIKLLEMGHHDSKIIVTTQTEEIAKDIDVHGYKTEIQSKKNLA 300

Query: 330 NGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNLKKNPVAGDRESEHSRS 389
           NGD VSA  QSLTKNTQNQAVPGSQTEETA AIAN IYE E+NL+KNPVAGD ESE SR 
Sbjct: 301 NGDEVSADKQSLTKNTQNQAVPGSQTEETAGAIANMIYETEKNLEKNPVAGDGESESSRL 360

Query: 390 LTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQSSSRLFKEYAFR 449
           +T+DAQNL V IP+VTE             + D   Q IFKL+KLSKQSSS LFKEYAFR
Sbjct: 361 ITKDAQNLDVSIPQVTEC------------EPDRGYQTIFKLKKLSKQSSSLLFKEYAFR 420

Query: 450 NRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQEKLSLQEKKENG 509
           N  EV+NPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEW NI++KL  QEKKE+G
Sbjct: 421 NSPEVQNPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWKNIEKKLMPQEKKEDG 480

Query: 510 ILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQLWMANGFLHSPEEKNSTMENI 569
           IL+VLRVCYDQMPSQLKPCFL+CSQL NDRI SSNDMIQLWMANG LHSPEEKN  MENI
Sbjct: 481 ILHVLRVCYDQMPSQLKPCFLYCSQLPNDRIFSSNDMIQLWMANGLLHSPEEKNLAMENI 540

Query: 570 GEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPNEVTEIR 629
           G+KYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGS GNNH  E+TEIR
Sbjct: 541 GKKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAHKITQEQSEGSWGNNHSKEITEIR 600

Query: 630 SIAFQERNMVLPNASLTEKCIWTYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHGNTK 689
           SIAFQERNMVLPNASLTEKCIW YK LRLLYL NADLQEIPNSIGTLKYLRYLDLHGN K
Sbjct: 601 SIAFQERNMVLPNASLTEKCIWRYKCLRLLYLGNADLQEIPNSIGTLKYLRYLDLHGNQK 660

Query: 690 IKHLPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNS 749
           IKHLP+SIC+LQSLQTLIL SCSALEDLPKDIRNLISLRYLWVTTNKL L KNGVGTM S
Sbjct: 661 IKHLPDSICDLQSLQTLILDSCSALEDLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMTS 720

Query: 750 LRFLAIGGCNNLENLFERQNCLAGLETLMIYNCTTLKLLPDKMRYLKSLQNLMIWSCKQL 809
           LRFLAIGGC NLENLFER +CLAGLETLMIYNC TLKLLP++MRYLKSLQNLMIW CKQL
Sbjct: 721 LRFLAIGGCKNLENLFERPDCLAGLETLMIYNCNTLKLLPNEMRYLKSLQNLMIWRCKQL 780

Query: 810 TLNLKEVEFKLQRFTIKELPRVERLPQWLENSAETLRILQIINCPIRIMERQGIENYESL 869
           TLNL+EVEFKLQRFT+KELP+VE LP+WLENS ETLR LQIINCPIR+ME    + Y +L
Sbjct: 781 TLNLEEVEFKLQRFTVKELPKVESLPRWLENSIETLRTLQIINCPIRLMELPVDKKYRAL 840

Query: 870 ENTIIYGAVRFETTTPGYDFEHRNLAMRNGNEEMYIY 905
           E  +I+G+VRF+T  P YDFEHRNL +  GNE    Y
Sbjct: 841 EIFLIHGSVRFDTM-PDYDFEHRNLTIFRGNEMNIHY 864

BLAST of Chy4G084330 vs. NCBI nr
Match: XP_038904479.1 (disease resistance protein RGA2-like [Benincasa hispida])

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 597/916 (65.17%), Postives = 691/916 (75.44%), Query Frame = 0

Query: 1   MAHCIYTQAENILTKLKYCPPYQKRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNV 60
           MAH IY QAENILTKLK CP YQ RIEYTM SLKA+LLDAE+KQEQNQ+L +WL ELQNV
Sbjct: 1   MAHFIYLQAENILTKLKDCPTYQNRIEYTMLSLKAILLDAEKKQEQNQYLHDWLKELQNV 60

Query: 61  FYQVENSIDEFKWEVFKQKDIGKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRM 120
           FYQ+E+SIDEFKWE FKQ+   KQV AHFSCS +ISANKL+Q  K ++ CD+LN I  RM
Sbjct: 61  FYQIEDSIDEFKWEFFKQEGTRKQVCAHFSCSAQISANKLRQTRKMEKLCDQLNAITARM 120

Query: 121 YEFHLKVKHIDSTSMETTHTFPSASEISIRHLKPSWQLRYPLTDASR------QYDNIYD 180
           YEFHLK KHI S SMETTH F SA+EISIRH+KPSWQL YPLTDA R      QY+  Y 
Sbjct: 121 YEFHLKEKHIASLSMETTHPFLSATEISIRHMKPSWQLLYPLTDAPRDDDFAEQYNQRYK 180

Query: 181 GILNDFNKCAH-VFHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVK 240
            +L+DFN   H  FHIVGEAG+GKST+A+FLYNH  V+   TSRYWVCVEEGF+T RL+K
Sbjct: 181 DLLSDFNGSTHRFFHIVGEAGMGKSTLAKFLYNHKSVIEKYTSRYWVCVEEGFDTQRLMK 240

Query: 241 EVYSHADNKEICEDLTTEQLLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMG 300
           EVY+HADNKEICE+LT +QLLSKV+R LK  TFL++FQDLSITNL D    +L  +L+MG
Sbjct: 241 EVYNHADNKEICEELTMKQLLSKVRRSLKERTFLLIFQDLSITNLGDCD--ILKNILEMG 300

Query: 301 QHGSKIIVTTQTEEIANDYE----DHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAV 360
            H SKIIVTTQ+EEIA   +         TE++SK+ L   D  S  N S+  + QNQAV
Sbjct: 301 NHDSKIIVTTQSEEIAKALKLERVYRWPMTEKQSKQKLVANDGESVGNSSIAVDAQNQAV 360

Query: 361 PGSQTEETADAIANKIYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAV 420
           PG                                 H       + +L             
Sbjct: 361 PGP--------------------------------HDTGPIRFSYDLR------------ 420

Query: 421 FNPVIPPRKQDDTVCQIIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKC 480
           F+P IP  +QD    Q I+ LE+LS+++S  LFK YAF++ QE E  E+T+I  Q+++KC
Sbjct: 421 FHPSIPQNEQDVMTYQTIYTLERLSEENSILLFKHYAFKDGQEAEKTEITEIAKQIVQKC 480

Query: 481 MGVPLAIKCLGSLLSSKTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFL 540
            GVPLAIKCLG+LLSSKT IAEW N++ KL   ++K++ IL +LR+CY+QMPSQLKPCFL
Sbjct: 481 KGVPLAIKCLGNLLSSKTRIAEWQNVENKLRSLQEKDDSILPILRLCYNQMPSQLKPCFL 540

Query: 541 HCSQLSNDRIISSNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHG 600
            CSQL NDRI+SSNDMIQLWMA+G LHSPEE N +MENIGEKYFMELWSRCFIQEIEEHG
Sbjct: 541 LCSQLPNDRILSSNDMIQLWMAHGLLHSPEEINLSMENIGEKYFMELWSRCFIQEIEEHG 600

Query: 601 LGYWIKLHPLIQKLAHKITQEQSEGSGGNNHPNEVTEIRSIAFQERNMVLPNASLTEKCI 660
           LGYW+K+HPLI KLAH +TQEQSEGS G   P   TEIRSIAFQ RNMVLPNASL EKCI
Sbjct: 601 LGYWVKVHPLIIKLAHVLTQEQSEGSEGTKPPKPFTEIRSIAFQVRNMVLPNASLPEKCI 660

Query: 661 WTYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGS 720
             +K LRLLYL NA+LQEIPNSIGTLK LRYLDLHGN KIK LPN+IC+L SLQTLIL S
Sbjct: 661 SKFKCLRLLYLGNANLQEIPNSIGTLKNLRYLDLHGNQKIKQLPNAICDLLSLQTLILES 720

Query: 721 CSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERQNC 780
           CS+LE LPKDIRNLISLRYLWVTTNKL L KNGVGTM+SLRFLAIGGC+NL+NLFE+ +C
Sbjct: 721 CSSLEGLPKDIRNLISLRYLWVTTNKLHLHKNGVGTMSSLRFLAIGGCHNLQNLFEQPDC 780

Query: 781 LAGLETLMIYNCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPR 840
           LAGLETLMIY+C TLKLLP++MR+LKSLQNLMIWSCKQLTL L+ VEF+LQRFTIKELP+
Sbjct: 781 LAGLETLMIYDCNTLKLLPNEMRFLKSLQNLMIWSCKQLTLTLEAVEFRLQRFTIKELPK 840

Query: 841 VERLPQWLENSAETLRILQIINCPIRIMERQGIENYESLENTIIYGAVRFETTTPGYDFE 900
           VE LP+WLE SAETLR LQIINCPIR+       +  ++E  IIYGAVRFE T PGY FE
Sbjct: 841 VEELPRWLEGSAETLRSLQIINCPIRMGGM--FVSLNAVERIIIYGAVRFEMTAPGYCFE 868

Query: 901 HRNLAMRNGNEEMYIY 905
           HRN  +  G  E   Y
Sbjct: 901 HRNFVIGGGASEKMKY 868

BLAST of Chy4G084330 vs. NCBI nr
Match: XP_022154274.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 900 bits (2325), Expect = 0.0
Identity = 492/854 (57.61%), Postives = 606/854 (70.96%), Query Frame = 0

Query: 1   MAHCIYTQAENILTKLKYCPPYQKRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNV 60
           MAH IY++AENIL KLK CP   +R+EYTM SLKA+LLDAEEKQEQN  LRNWL ELQ+V
Sbjct: 41  MAHFIYSRAENILNKLKDCPIDLRRMEYTMLSLKAILLDAEEKQEQNHGLRNWLKELQDV 100

Query: 61  FYQVENSIDEFKWEVFKQKDIGKQVLAHFSCSNRISANK-LKQKCKRKQACDELNKIATR 120
           FYQ E+SIDE KWE   Q+ IG++V A FSCS++ +A+K      K K+ CD L++IA++
Sbjct: 101 FYQTEDSIDELKWE---QEGIGRKVRARFSCSDKTAAHKKTNPTSKMKKLCDHLDEIASK 160

Query: 121 MYEFHLKVKHIDSTSMETTHTFPSASEISIRHLKPSWQLRYPLTDASRQYDNI-YDGILN 180
           MYEFHL  +H  S  MET H+F SA+E+S RH++PSWQL YPLTDA   Y N  Y  +LN
Sbjct: 161 MYEFHLTEEHTSSIRMETAHSFLSATEVSTRHMRPSWQLFYPLTDAPNSYCNSRYVELLN 220

Query: 181 DF-NKCAHVFHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYS 240
           DF +     FHIVGEAGIGKST+A+F Y+H+DV  + TSR WVCV++G+NT RL+KEVYS
Sbjct: 221 DFIHSKQQFFHIVGEAGIGKSTLAKFFYSHHDVCSSFTSRSWVCVKDGYNTKRLLKEVYS 280

Query: 241 HADNKEICEDLTTEQLLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGS 300
             D+KE+ +D T +QLL KV+R L+ +TFL+VFQDLS  NL+D  S +L  LL++G +GS
Sbjct: 281 FQDDKEVRDDFTKKQLLLKVQRFLRGQTFLVVFQDLSSKNLEDDRS-MLKNLLELGNYGS 340

Query: 301 KIIVTTQTEEIANDYEDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEET 360
           KIIVTTQ EEIA+D +                                            
Sbjct: 341 KIIVTTQNEEIADDLK-------------------------------------------- 400

Query: 361 ADAIANKIYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPR 420
                 K+Y+ ++  ++N V          ++   A N+   +  ++E   V        
Sbjct: 401 ----LKKVYKTKKLSEQNQV---------NTIIHQAINI---VEGLSEQNQV-------- 460

Query: 421 KQDDTVCQIIFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIK 480
             +    Q I  +E LS+Q+S  LFK+YAFR+ +E +N EL  I  Q++EKC   PLAIK
Sbjct: 461 --NTIEHQAIIIVEGLSEQNSFSLFKDYAFRDTKEAQNLEL--IAKQIVEKCKRFPLAIK 520

Query: 481 CLGSLLSSKTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSND 540
           C+GSLLSSK  IAEW  +  KL  QE+++  IL +LRVCY+ MPS LKPCFL  SQL +D
Sbjct: 521 CVGSLLSSKNRIAEWNEVNNKLCAQEEEDGSILPILRVCYNLMPSYLKPCFLLYSQLPDD 580

Query: 541 RIISSNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLH 600
           RI+SSND+IQLWMA+G LHSP+E + ++EN GEKYFMELWSRCF+QE+EEHGLG W KLH
Sbjct: 581 RILSSNDVIQLWMAHGLLHSPKENSLSLENTGEKYFMELWSRCFVQEMEEHGLGCWFKLH 640

Query: 601 PLIQKLAHKITQEQSEGSGGNNHPNEVTEIRSIAFQERNMVLPNASLTEKCIWTYKGLRL 660
           PLI+KLAH +T+EQ+EG  G       TEIRSIAF+ R+ V P+  L  KCI  +K LRL
Sbjct: 641 PLIRKLAHVLTREQTEGLVGTKPD---TEIRSIAFRVRDKVKPSVFLANKCISMFKCLRL 700

Query: 661 LYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQTLILGSCSALEDLP 720
           LYL NADLQEIPN+IGTLKYLRYLDL GN +IK LPNSIC LQSLQTLIL SCS+L +LP
Sbjct: 701 LYLGNADLQEIPNAIGTLKYLRYLDLQGNERIKQLPNSICKLQSLQTLILDSCSSLVELP 760

Query: 721 KDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLAIGGCNNLENLFERQNCLAGLETLM 780
            DI NLISLRYLWVT+NKLRL KNGVGTM SLRFLAIGGC+NL+ LFER +CLAGLETLM
Sbjct: 761 NDIANLISLRYLWVTSNKLRLHKNGVGTMTSLRFLAIGGCDNLQILFERPDCLAGLETLM 814

Query: 781 IYNCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEVEFKLQRFTIKELPRVERLPQWL 840
           IYNC  LK LP++MRYLKSLQNL+IW CKQL L LKEVEFKLQRFTI+ELP+VE LPQWL
Sbjct: 821 IYNCNALKSLPNEMRYLKSLQNLVIWGCKQLKLTLKEVEFKLQRFTIRELPKVEELPQWL 814

Query: 841 ENSAETLRILQIIN 851
           + S+ETLR  Q +N
Sbjct: 881 QGSSETLRF-QYVN 814

BLAST of Chy4G084330 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 183.3 bits (464), Expect = 8.5e-46
Identity = 193/781 (24.71%), Postives = 321/781 (41.10%), Query Frame = 0

Query: 24  KRIEYTMSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEVFKQKDIG- 83
           +R+   + ++ AVL+DAEEKQ  N  +  W+ EL++V Y  E+++D+   E  +  +IG 
Sbjct: 40  ERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALR-LNIGA 99

Query: 84  --------KQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTS 143
                   +Q+    S  + +  N    + + ++    L ++A++     LK    + T+
Sbjct: 100 ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLK----ELTA 159

Query: 144 METTHTFPSASEISIRHLKPSWQLRYPLTDASRQY--DNIYDGIL------NDFNKCAHV 203
           M      P+ S                L D S  +  D+  D I+      N  +    V
Sbjct: 160 MIPKQRLPTTS----------------LVDESEVFGRDDDKDEIMRFLIPENGKDNGITV 219

Query: 204 FHIVGEAGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICE 263
             IVG  G+GK+T+++ LYN   V     ++ W  V E F+  ++ K+VY    ++  CE
Sbjct: 220 VAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRP-CE 279

Query: 264 DLTTEQLLSKVKRLLKVE--TFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQ 323
               + L  K+K  L      FL+V  DL   N  D    +L Q       GS+I+VTT+
Sbjct: 280 FTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWD--LLRQPFIHAAQGSQILVTTR 339

Query: 324 TEEIANDYEDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANK 383
           ++ +A+                                                      
Sbjct: 340 SQRVAS------------------------------------------------------ 399

Query: 384 IYEIEQNLKKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVC 443
                                                                     +C
Sbjct: 400 ---------------------------------------------------------IMC 459

Query: 444 QI-IFKLEKLSKQSSSRLFKEYAFRNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLL 503
            + +  L+ LS      LF +  F N++   N E+  + ++++ KC G+PLA+K LG +L
Sbjct: 460 AVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVL 519

Query: 504 SSKTSIAEWTNIQEK--LSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIIS 563
             +  + EW  +       L   K N +L VLRV Y  +P+ LK CF +CS         
Sbjct: 520 RFEGKVIEWERVLSSRIWDLPADKSN-LLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFE 579

Query: 564 SNDMIQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQ 623
            + ++ LWMA GFL      +  +E +G +YF EL SR  +Q+ +   +     +H  I 
Sbjct: 580 KDKVVLLWMAEGFLQQ-TRSSKNLEELGNEYFSELESRSLLQKTKTRYI-----MHDFIN 639

Query: 624 KLAHKITQE-QSEGSGG----------------NNH--PNEVTEIRSIAFQER--NMVLP 683
           +LA   + E  S+   G                +N+  P E   +R + F      + L 
Sbjct: 640 ELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLT 677

Query: 684 NAS--------LTEKCIWTYKGLRLLYLSNADLQEI-PNSIGTLKYLRYLDLHGNTKIKH 743
           N+S        ++EK + T   LR+L LS+  +  + P+    + + R+LDL   T+++ 
Sbjct: 700 NSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDL-SRTELEK 677

Query: 744 LPNSICNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRF 753
           LP S+C + +LQTL+L  CS+L++LP DI NLI+LRYL +   KLR      G + SL+ 
Sbjct: 760 LPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRFGRLKSLQT 677

BLAST of Chy4G084330 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 147.1 bits (370), Expect = 6.7e-35
Identity = 110/357 (30.81%), Postives = 173/357 (48.46%), Query Frame = 0

Query: 427 IFKLEKLSKQSSSRLFKEYAFRNRQEVE-NPELTKIVDQLLEKCMGVPLAIKCLGSLLSS 486
           I++++ ++ +    L   +AF N      N EL  I  ++ E+C G+PLA + + S L S
Sbjct: 323 IYQMKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRS 382

Query: 487 KTSIAEWTNIQEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDM 546
           K +  +W  + +  S      N IL VL++ YD +P QLK CF  CS      +    ++
Sbjct: 383 KPNPDDWYAVSKNFS---SYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDREEL 442

Query: 547 IQLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIKLHPLIQKLAH 606
           + LWMA   L+ P   +  +E+IG  Y  +L ++ F Q ++     +   +H L+  LA 
Sbjct: 443 VLLWMAIDLLYQP-RSSRRLEDIGNDYLGDLVAQSFFQRLDITMTSF--VMHDLMNDLAK 502

Query: 607 KITQEQSEGSGGNNHPNEVTEIR-----------SIAFQE------RNMVLP-------- 666
            ++ +       +N P   +  R           S+AF+          +LP        
Sbjct: 503 AVSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLE 562

Query: 667 NASLTEK----CIWTYKGLRLLYLSNADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSI 726
           +  LTEK     +    GLR+L LS+  +  +P S+  LK LRYLDL  +TKIK LP  +
Sbjct: 563 SLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDL-SSTKIKELPEFV 622

Query: 727 CNLQSLQTLILGSCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLA 754
           C L +LQTL+L +C  L  LPK I  LI+LR L +    L     G+  + SL+ L+
Sbjct: 623 CTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPGIKKLRSLQKLS 672

BLAST of Chy4G084330 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 129.8 bits (325), Expect = 1.1e-29
Identity = 169/769 (21.98%), Postives = 304/769 (39.53%), Query Frame = 0

Query: 22  YQKRIEYTMSSLK---AVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEVFKQ 81
           Y+K++E   S LK   + L DAE ++  N+ LR  + +L+ + Y+ E+ + + +      
Sbjct: 27  YRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQ---LAD 86

Query: 82  KDIGKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETT 141
            D G +  +  +  +R+   ++  + K+ +   E+N+  T++            + +E  
Sbjct: 87  GDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKI-----------KSQVEPY 146

Query: 142 HTFPSASEISIRHLKPSWQLRYPLTDASR------QYDNIYDGILNDFNKCAHVFHIVGE 201
             F + S +   +    W    P+ D ++          I + +    +    +   VG 
Sbjct: 147 FEFITPSNVGRDNGTDRWS--SPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLLIMAFVGM 206

Query: 202 AGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQ 261
            G+GK+T+A+ ++N  ++      R WV V + F   ++++ +  +  +  + +D+ T  
Sbjct: 207 GGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDIGT-- 266

Query: 262 LLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDY 321
           LL K+++ L  + +LIV  D+   NL       +YQ L  GQ GS +IVTT++E +A   
Sbjct: 267 LLRKIQQYLLGKRYLIVMDDVWDKNLSWWDK--IYQGLPRGQGGS-VIVTTRSESVAKRV 326

Query: 322 EDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNL 381
           +    KT R                                                   
Sbjct: 327 QARDDKTHRP-------------------------------------------------- 386

Query: 382 KKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEK 441
                                                                     E 
Sbjct: 387 ----------------------------------------------------------EL 446

Query: 442 LSKQSSSRLFKEYAF-RNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSI-A 501
           LS  +S  LF   AF  N    E PEL  +  +++ KC G+PL IK +G LL  K  +  
Sbjct: 447 LSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYH 506

Query: 502 EWTNI----QEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMI 561
           EW  I    Q++L     + + ++  L++ YD++PS LK C L  S    D +I    ++
Sbjct: 507 EWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLV 566

Query: 562 QLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWI--KLHPLIQKLA 621
             W+  GF+     +++T    GE  F  L +RC I+ +++   G  I  K+H +++ L 
Sbjct: 567 HGWIGEGFVMWRNGRSAT--ESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLV 626

Query: 622 HKITQEQS-----------EGSGGNNHPNEVT---EIRSIAFQERNMVLP--NASLTEKC 681
             I ++ S            G  GN    ++    ++R +    +   +   N+ L +K 
Sbjct: 627 IDIAKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKK- 663

Query: 682 IWTYKGLRLLYLS----NADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQT 741
               K LR+L +S    +A L EI + I +L++L  L L     +   P S+ +L +LQ 
Sbjct: 687 FTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQI 663

Query: 742 LILGSCSALEDLPKDIRNLISLRYLWVTT-NKLRLDKNGVGTMNSLRFL 753
           L    C  L+ L   I     L  L +T    L     G+G++  L  L
Sbjct: 747 LDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVL 663

BLAST of Chy4G084330 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 129.8 bits (325), Expect = 1.1e-29
Identity = 169/769 (21.98%), Postives = 304/769 (39.53%), Query Frame = 0

Query: 22  YQKRIEYTMSSLK---AVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEVFKQ 81
           Y+K++E   S LK   + L DAE ++  N+ LR  + +L+ + Y+ E+ + + +      
Sbjct: 27  YRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQ---LAD 86

Query: 82  KDIGKQVLAHFSCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDSTSMETT 141
            D G +  +  +  +R+   ++  + K+ +   E+N+  T++            + +E  
Sbjct: 87  GDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKI-----------KSQVEPY 146

Query: 142 HTFPSASEISIRHLKPSWQLRYPLTDASR------QYDNIYDGILNDFNKCAHVFHIVGE 201
             F + S +   +    W    P+ D ++          I + +    +    +   VG 
Sbjct: 147 FEFITPSNVGRDNGTDRWS--SPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLLIMAFVGM 206

Query: 202 AGIGKSTVARFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQ 261
            G+GK+T+A+ ++N  ++      R WV V + F   ++++ +  +  +  + +D+ T  
Sbjct: 207 GGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDIGT-- 266

Query: 262 LLSKVKRLLKVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDY 321
           LL K+++ L  + +LIV  D+   NL       +YQ L  GQ GS +IVTT++E +A   
Sbjct: 267 LLRKIQQYLLGKRYLIVMDDVWDKNLSWWDK--IYQGLPRGQGGS-VIVTTRSESVAKRV 326

Query: 322 EDHGQKTERRSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNL 381
           +    KT R                                                   
Sbjct: 327 QARDDKTHRP-------------------------------------------------- 386

Query: 382 KKNPVAGDRESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEK 441
                                                                     E 
Sbjct: 387 ----------------------------------------------------------EL 446

Query: 442 LSKQSSSRLFKEYAF-RNRQEVENPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSI-A 501
           LS  +S  LF   AF  N    E PEL  +  +++ KC G+PL IK +G LL  K  +  
Sbjct: 447 LSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHVYH 506

Query: 502 EWTNI----QEKLSLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMI 561
           EW  I    Q++L     + + ++  L++ YD++PS LK C L  S    D +I    ++
Sbjct: 507 EWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQLV 566

Query: 562 QLWMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWI--KLHPLIQKLA 621
             W+  GF+     +++T    GE  F  L +RC I+ +++   G  I  K+H +++ L 
Sbjct: 567 HGWIGEGFVMWRNGRSAT--ESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLV 626

Query: 622 HKITQEQS-----------EGSGGNNHPNEVT---EIRSIAFQERNMVLP--NASLTEKC 681
             I ++ S            G  GN    ++    ++R +    +   +   N+ L +K 
Sbjct: 627 IDIAKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKK- 663

Query: 682 IWTYKGLRLLYLS----NADLQEIPNSIGTLKYLRYLDLHGNTKIKHLPNSICNLQSLQT 741
               K LR+L +S    +A L EI + I +L++L  L L     +   P S+ +L +LQ 
Sbjct: 687 FTDCKYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQI 663

Query: 742 LILGSCSALEDLPKDIRNLISLRYLWVTT-NKLRLDKNGVGTMNSLRFL 753
           L    C  L+ L   I     L  L +T    L     G+G++  L  L
Sbjct: 747 LDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVL 663

BLAST of Chy4G084330 vs. TAIR 10
Match: AT1G59780.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 113.6 bits (283), Expect = 8.2e-25
Identity = 174/863 (20.16%), Postives = 325/863 (37.66%), Query Frame = 0

Query: 30  MSSLKAVLLDAEEKQEQNQHLRNWLMELQNVFYQVENSIDEFKWEVFKQKDIGKQVLAHF 89
           +  L A L DA+ K++     RN L E++ + Y  E+ I+ F      +  +  + LA F
Sbjct: 43  LKMLMAFLSDADAKKQTRALARNCLEEIKEITYDAEDIIEIF----LLKGSVNMRSLACF 102

Query: 90  SCSNRISANKLKQKCKRKQACDELNKIATRMYEFHLKVKHIDST--------SMETTHTF 149
               R  A ++    KR      ++K+   M    +K   +D            E  HTF
Sbjct: 103 PGGRREIALQITSISKR------ISKVIQVMQNLGIKSDIMDGVDSHAQLERKRELRHTF 162

Query: 150 PSASEISIRHLKPSWQLRYPLTDASRQYDNIYDGILNDFNKCAHVFHIVGEAGIGKSTVA 209
            S SE +             L    +  + + + ++   N  +H   I G  G+GK+T+A
Sbjct: 163 SSESESN-------------LVGLEKNVEKLVEELVG--NDSSHGVSITGLGGLGKTTLA 222

Query: 210 RFLYNHNDVVGNCTSRYWVCVEEGFNTHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLL 269
           R +++H+ V  +     WVCV + F    + K +  +   K    DL  + +  K+ +LL
Sbjct: 223 RQIFDHDKVKSHFDGLAWVCVSQEFTRKDVWKTILGNLSPKYKDSDLPEDDIQKKLFQLL 282

Query: 270 KVETFLIVFQDLSITNLDDRSSFVLYQLLKMGQHGSKIIVTTQTEEIANDYEDHGQKTER 329
           + +  LIVF DL          + +  +    + G K+++T++ + I             
Sbjct: 283 ETKKALIVFDDL----WKREDWYRIAPMFPERKAGWKVLLTSRNDAI------------- 342

Query: 330 RSKENLANGDRVSAHNQSLTKNTQNQAVPGSQTEETADAIANKIYEIEQNLKKNPVAGDR 389
                                                                       
Sbjct: 343 ------------------------------------------------------------ 402

Query: 390 ESEHSRSLTEDAQNLGVPIPKVTEDTAVFNPVIPPRKQDDTVCQIIFKLEKLSKQSSSRL 449
              H   +T                                     FK E L+     +L
Sbjct: 403 ---HPHCVT-------------------------------------FKPELLTHDECWKL 462

Query: 450 FKEYAFRNRQEVE----NPELTKIVDQLLEKCMGVPLAIKCLGSLLSSKTSIAEWTNIQE 509
            +  AF  ++ +     + E+ K+  ++ + C  +PLA+K LG LL +K ++ +W  I E
Sbjct: 463 LQRIAFSKQKTITGYIIDKEMVKMAKEMTKHCKRLPLAVKLLGGLLDAKHTLRQWKLISE 522

Query: 510 KL---------SLQEKKENGILYVLRVCYDQMPSQLKPCFLHCSQLSNDRIISSNDMIQL 569
            +         S  E   + + +VL + ++ +P  LK C L+ +    D  I    +  +
Sbjct: 523 NIISHIVVGGTSSNENDSSSVNHVLSLSFEGLPGYLKHCLLYLASYPEDHEIEIERLSYV 582

Query: 570 WMANGFLHSPEEKNSTMENIGEKYFMELWSRCFIQEIEEHGLGYWIK--LHPLIQKLAHK 629
           W A G  +    + +T+ ++ + Y  EL  R  +    +     + K  LH L++++   
Sbjct: 583 WAAEGITYPGNYEGATIRDVADLYIEELVKRNMVISERDALTSRFEKCQLHDLMREICLL 642

Query: 630 ITQEQSEGSGGNNHPNEVTEIRSIA-FQERNMVLPNASL-----------TEKCIWTYKG 689
             +E++      + P   + + S+A  + R +V+ N S+               ++   G
Sbjct: 643 KAKEENFLQIVTD-PTSSSSVHSLASSRSRRLVVYNTSIFSGENDMKNSKLRSLLFIPVG 702

Query: 690 ---------------LRLLYLSNADLQ--EIPNSIGTLKYLRYLDLHGNTKIKHLPNSIC 749
                          LR+L L  A  +  ++P+SIG L +L+YL L+    + +LP+S+ 
Sbjct: 703 YSRFSMGSNFIELPLLRVLDLDGAKFKGGKLPSSIGKLIHLKYLSLY-QASVTYLPSSLR 755

Query: 750 NLQSLQTLILG-SCSALEDLPKDIRNLISLRYLWVTTNKLRLDKNGVGTMNSLRFLA-IG 809
           NL+SL  L L  +   L ++P   + ++ LRYL +   +  L K  +G +  L  L    
Sbjct: 763 NLKSLLYLNLRINSGQLINVPNVFKEMLELRYLSLPWERSSLTKLELGNLLKLETLINFS 755

Query: 810 GCNNLENLFERQNCLAGLETLMIYNCTTLKLLPDKMRYLKSLQNLMIWSCKQLTLNLKEV 838
             ++      R   L  L+ L+      ++ L   +  L  L++L +      T +   V
Sbjct: 823 TKDSSVTDLHRMTKLRTLQILISGEGLHMETLSSALSMLGHLEDLTV------TPSENSV 755