Chy4G084080 (gene) Cucumber (hystrix) v1

Overview
NameChy4G084080
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionE3 ubiquitin-protein ligase
LocationchrH04: 23312547 .. 23315769 (+)
RNA-Seq ExpressionChy4G084080
SyntenyChy4G084080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAACAACGAGCTCTCAGACCCACTCTCAATCACTCTCTTCTTCGTTATCCCACTGTCAGCACTACTTTCACAATCCCTTTAACTCGCTACACTACCCGTTTGATTTCCTCTGACCGCCACCGTCCTCTACTCGCCGATTGCGCCGAAGATCACTCTGACTACGATGATTTCAGTCGTCGCTCTTTTATTAGGGAGAATTGCTCCTACTCTATGCCCATTTTGGTTCTTGACGTTGTCTGGAATTTGGCTTTTGTTCTTGTCTCTGTTGTTGTCCTTTTCTCTACGTTTAGAGAACGACCTTCTACCCCTTTACGCCTTTGGATTTCTGGGTATGGCTTACAATGCCTTTTGCATGTTGGTTTTGTCTTCTTTGAGTACCAGAGGAGTATGGCACACCATGGATTTGAGGACCGTGCGGCTCATCGCAGGTAGGGAAAATAGTGTTTATGGTTTTGTTTCATGGTGCTAGAGGAAGTTCTTTGTCATGCGCTGTCTCTGGTTGTCTACATTGTTGACCTTTTTCTAATCCCTTGAATTTTGAGCTGAAGACACTAATTTTTGGTCTTGTGCGTTTTTTTTTTCCTTCTGGAAACCCCTTCTTGTTTGATGGGAGAAACAGTGAACTATTGTGTAATTGAACATGGGTTTTCTTAGAAACGGTTGGGTAGCGGCAATGCATTTTCTTTGTTAGGCCTTCATATGTGGAAGAAGTAAAAGCGAAGATAGTTGAATAACTTGAATTGATTGCATTACATGTGCAATTTTGAGGTTTTCCTTTCCAATGCAGTAATCTAAGCAACATGTGTACCAATTTCACTCATCTTTTGTCCTTTTGAAGTTTGGTTTTGACCTGTTTTTCCTTTGAATCCAGTGGTATAGACATTGCATCTAGTTGTTCATTTTTTGTTGATTTCAATCACAGAAGTATTTTGTGTATAAAGTACTGAGGATAGTTTATATCACCTCCCGTAGGGTTCTCTTGGTGTTTGATTTATTAAAATATACTAAACAGGGAGGGAGAACTTAATACAAAAGGGAATGCCAGGTCTTGTAGAGTTGAGCGATTTGAACAGTTTTAGGGACTTGTCTTATGAGGAATGGATTAGTATTTTAAGAAAATAGTTTGATTTGGCATAAGTGCAGAGTATAATTCATTTCTCCTCTTTTTCTTTTTTAGTTTGTTTTTTCCTATGTAGAAATTGATACCTCCCATTTAATGCTGCTATCAGATGGAATTATAGTTGTTTCCATATTCTGGTTTTTTCTATTTTGCCTGTTGGTATTTGTAATATTGGGAAATGCTTGAAGGAAGTGTAGGTGACCTCTTAGAGAGAAAACATCTGTTATCTTCCTTTGGCTTTTTAATGAGTTTAAATTTTATTTCTTTTTATATTTGTTTTTGGACTCTATTTCTCTAAGTTTAACCTTTAAAATGGATAATAATGATGGAATTCTCTTTCTTACCCCCTTGGTGAATTCACAATCTATATGTTTCTTCTATATGTCAGAAAAGCCAAATTTGCTCAACGTATGGAAAATTCAAAACTGCCAAACGTATTGGCTACAGTTAATTGTATTCTTTTACGAATGGATATGCATCCCATTGTGTTCTCACATTGAGTCTTGAACTTGCTTAATAAACACAGAAAAATATACAGATCTTTTAGCTGCAACAAACTTTCGACTAATTTGTTCTTATATGTCCTTCTAGCATAATGAAAAGGTTGGAGTCAATGAATACAATGACTTCGTCTGTCTGGTGGGTCTTCGGATTCTATTGGATTGTCATGGGTGGCCAAGCACTTCTACAAGATTCTCCCCGGCTTTACTGGTAAGAACTTTTCCTTTTTGACAGTGACATGTTTCCTCATGGAGCAATAGGTTTATATACCAAATTGGAAATTTGCAACTTCAACTACATTGAAAGTTGTTGCAACTGCAATCTCACGTTTTTGCACTGTTCAATCTGGACTCAACTTTAAATTTTTCTTCACAAGTTCATAACCTTTCAGCAGATGTCATAGTAGAAAACTGGTGGTTAAGTTATTAATCTTTTCAGATTAGACTTCTAGTAATGTTTACATATCAAAACAAGCTGTTGAGATTTAGATCTCAGACAATTACAAAATCGCCTATGCAAAAAGCAAGGTATATCTTCAGTATACTGTTTTTTTTTAATAACAAAAAGAAACAGAAGAATTAAACCCATGATCCTATAAGGATAAGTGGATAACGATGTAAGAAAAAACAACCCTCAATTACAATCAACATAAATGTTTCACACAGACTCAACACTGATAACAAGTTTAAGGAAAACTAAGAAGATGATTTGAACTTTTCTGAATCCATTTCCTCGATCCAATCTGAAGATGTCATCAAATGTTTTTCTCTTTCCATTATTTCCAAATTGTAGCTTTAATTGGATTCACCCAAAGCAATCTGGCTTTGATTTGTTGGTGTGCGGAGCATTTAATCTAATCTAATTTGGTGCTTCGATGCTGATATTTTTCGGGGTCAATTGCAGGTTAACTGTGGTCTTTCTAGCGTTTGACTTGTTCTTCATTCTCTTTTGTATTGGGATGGCATGTGTTATTTTCTTCTCTCTTTGTTGCTGCATTCCAATTGTAGCATTTGCATATGCCATGACTACTAGAGAAGGTGCCTCAGAAGAGGATATCGGCACTCTTCCCAAGTATACATTTCGTCAGGCTGCTGTACTTGGAACATTTAACCTTGGAAAAGAACGAGAACCAATCGGTTCAACTGTGGAGGTAGATAACGTTCACCGTATCAAAGAACTTGCTCTTCATCCAGAGGATTCGGTCAGCTACTTATCTTTTATGTCTAATTATGAATATCTATAAGCTAGCTATCTAGTGGATTGCTACAATTCTATTGTATCATCATAGAAACATTATCACAATAGACAAGATGTAATCAAATTATAGTGTTTGTCACACTCACTATAGTGTTCATCTTGTAATTGTTTTCGCTTATCTCATCGAGGGTTTCTCCTAATTTGAAGCTGAGTATGCAAAAATTTACTTTTCTATAGGAATGTTGCATTTGCCTTTCAAGATATGAAGACGGGACGGAGCTCTATACTCTACCCTGCAACCACCATTTTCATTGTGGGTGCATTGCCAAATGGCTTCGGATAAATGCAACCTGCCCACTCTGCAAGTCTAACATCCGCCGGGGTGATACATTGGTTTGA

mRNA sequence

ATGGAAGAACAACGAGCTCTCAGACCCACTCTCAATCACTCTCTTCTTCGTTATCCCACTGTCAGCACTACTTTCACAATCCCTTTAACTCGCTACACTACCCGTTTGATTTCCTCTGACCGCCACCGTCCTCTACTCGCCGATTGCGCCGAAGATCACTCTGACTACGATGATTTCAGTCGTCGCTCTTTTATTAGGGAGAATTGCTCCTACTCTATGCCCATTTTGGTTCTTGACGTTGTCTGGAATTTGGCTTTTGTTCTTGTCTCTGTTGTTGTCCTTTTCTCTACGTTTAGAGAACGACCTTCTACCCCTTTACGCCTTTGGATTTCTGGGTATGGCTTACAATGCCTTTTGCATGTTGGTTTTGTCTTCTTTGAGTACCAGAGGAGTATGGCACACCATGGATTTGAGGACCGTGCGGCTCATCGCAGCATAATGAAAAGGTTGGAGTCAATGAATACAATGACTTCGTCTGTCTGGTGGGTCTTCGGATTCTATTGGATTGTCATGGGTGGCCAAGCACTTCTACAAGATTCTCCCCGGCTTTACTGGTTAACTGTGGTCTTTCTAGCGTTTGACTTGTTCTTCATTCTCTTTTGTATTGGGATGGCATGTGTTATTTTCTTCTCTCTTTGTTGCTGCATTCCAATTGTAGCATTTGCATATGCCATGACTACTAGAGAAGGTGCCTCAGAAGAGGATATCGGCACTCTTCCCAAGTATACATTTCGTCAGGCTGCTGTACTTGGAACATTTAACCTTGGAAAAGAACGAGAACCAATCGGTTCAACTGTGGAGGTAGATAACGTTCACCGTATCAAAGAACTTGCTCTTCATCCAGAGGATTCGGAATGTTGCATTTGCCTTTCAAGATATGAAGACGGGACGGAGCTCTATACTCTACCCTGCAACCACCATTTTCATTGTGGGTGCATTGCCAAATGGCTTCGGATAAATGCAACCTGCCCACTCTGCAAGTCTAACATCCGCCGGGGTGATACATTGGTTTGA

Coding sequence (CDS)

ATGGAAGAACAACGAGCTCTCAGACCCACTCTCAATCACTCTCTTCTTCGTTATCCCACTGTCAGCACTACTTTCACAATCCCTTTAACTCGCTACACTACCCGTTTGATTTCCTCTGACCGCCACCGTCCTCTACTCGCCGATTGCGCCGAAGATCACTCTGACTACGATGATTTCAGTCGTCGCTCTTTTATTAGGGAGAATTGCTCCTACTCTATGCCCATTTTGGTTCTTGACGTTGTCTGGAATTTGGCTTTTGTTCTTGTCTCTGTTGTTGTCCTTTTCTCTACGTTTAGAGAACGACCTTCTACCCCTTTACGCCTTTGGATTTCTGGGTATGGCTTACAATGCCTTTTGCATGTTGGTTTTGTCTTCTTTGAGTACCAGAGGAGTATGGCACACCATGGATTTGAGGACCGTGCGGCTCATCGCAGCATAATGAAAAGGTTGGAGTCAATGAATACAATGACTTCGTCTGTCTGGTGGGTCTTCGGATTCTATTGGATTGTCATGGGTGGCCAAGCACTTCTACAAGATTCTCCCCGGCTTTACTGGTTAACTGTGGTCTTTCTAGCGTTTGACTTGTTCTTCATTCTCTTTTGTATTGGGATGGCATGTGTTATTTTCTTCTCTCTTTGTTGCTGCATTCCAATTGTAGCATTTGCATATGCCATGACTACTAGAGAAGGTGCCTCAGAAGAGGATATCGGCACTCTTCCCAAGTATACATTTCGTCAGGCTGCTGTACTTGGAACATTTAACCTTGGAAAAGAACGAGAACCAATCGGTTCAACTGTGGAGGTAGATAACGTTCACCGTATCAAAGAACTTGCTCTTCATCCAGAGGATTCGGAATGTTGCATTTGCCTTTCAAGATATGAAGACGGGACGGAGCTCTATACTCTACCCTGCAACCACCATTTTCATTGTGGGTGCATTGCCAAATGGCTTCGGATAAATGCAACCTGCCCACTCTGCAAGTCTAACATCCGCCGGGGTGATACATTGGTTTGA

Protein sequence

MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDYDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV*
Homology
BLAST of Chy4G084080 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.6e-71
Identity = 134/284 (47.18%), Postives = 175/284 (61.62%), Query Frame = 0

Query: 70  SYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQ 129
           +YS P++ LD++WNLAFV + V VL  +  E+P+ PLR+W+ GYG+QC LH+  V  EY+
Sbjct: 104 AYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYR 163

Query: 130 RSMAHH----------------------GFEDRAAHRSIMKRLESMNTMTSSVWWVFGFY 189
           R                             EDR    +  K LES NTM S +WW+ GFY
Sbjct: 164 RRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFY 223

Query: 190 WIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIP-IVAFAYAMT 249
           W+  GGQ L  DSP+LYWL ++FL FD+FF++FC+ +ACVI  ++CCC+P I+A  YA+ 
Sbjct: 224 WVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVA 283

Query: 250 TREGASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSEC 309
            +EGAS+ DI  +PK+ F +   +   + GK R   G   E      I E +L PED+EC
Sbjct: 284 DQEGASKNDIDQMPKFRFTKTGNVEKLS-GKAR---GIMTECGTDSPI-ERSLSPEDAEC 343

Query: 310 CICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNI 331
           CICL  YEDG EL  LPCNHHFHC CI KWL IN+ CPLCK NI
Sbjct: 344 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of Chy4G084080 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 4.7e-71
Identity = 142/313 (45.37%), Postives = 185/313 (59.11%), Query Frame = 0

Query: 70  SYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQ 129
           +YS P++VLD+VWNLAFV V+  +L  + +E P  PLR+W+ GY LQC+LH+  V  EY+
Sbjct: 95  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 154

Query: 130 R-----------------------------------SMAHHGFEDRA------AHRSIMK 189
           R                                   S  + G +D +         S+ K
Sbjct: 155 RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 214

Query: 190 RLESMNTMTSSVWWVFGFYWIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVI 249
            LES NTM S +WW+ GFYW+  GGQ L Q+SPR+YWL++VFL FD+FF++FC+ +ACVI
Sbjct: 215 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 274

Query: 250 FFSLCCCIP-IVAFAYAMTTREGASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGST-- 309
             ++CCC+P I+A  YA+  +EGAS+EDI  L K+ FR+   LG  N     E  G+T  
Sbjct: 275 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRK---LGDANKHTNDEAQGTTEG 334

Query: 310 --VEVDNVHRIKELALHPEDSECCICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATC 337
              E      I+   L  ED+ECCICLS YEDGTEL  LPC HHFHC C+ KWL INATC
Sbjct: 335 IMTECGTDSPIEHTLLQ-EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATC 394

BLAST of Chy4G084080 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 265.8 bits (678), Expect = 6.8e-70
Identity = 139/301 (46.18%), Postives = 178/301 (59.14%), Query Frame = 0

Query: 70  SYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQ 129
           +YS P++VLD VWNLAFV+V+  VL  +  E P+ PLR+WI GYGLQC++H+  V  EY+
Sbjct: 69  AYSKPVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYR 128

Query: 130 R--SMAHHGFEDRAA-----------------------------------HRSIMKRLES 189
           R  S        R++                                   + S  K LES
Sbjct: 129 RRNSRRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLES 188

Query: 190 MNTMTSSVWWVFGFYWIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSL 249
            NTM S +WWV GFYW+  GGQ L Q SP+LYWL +VFL FD+FF++FC+ +ACVI  ++
Sbjct: 189 ANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAV 248

Query: 250 CCCIP-IVAFAYAMTTREGASEEDIGTLPKYTFRQAAVLGTFNLGKE--REPIGSTVEVD 309
           CCC+P I+A  YA+  +EGAS+EDI  L K+ FR+        + +E  +   G  +   
Sbjct: 249 CCCLPCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTEC 308

Query: 310 NVHRIKELALHPEDSECCICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSN 331
                 E AL  ED+ECCICLS YED TEL  LPC HHFHCGC+ KWL INATCPLCK N
Sbjct: 309 GTDSPVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYN 368

BLAST of Chy4G084080 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.6e-69
Identity = 131/292 (44.86%), Postives = 180/292 (61.64%), Query Frame = 0

Query: 66  RENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVF 125
           R +  YS P++  D++WN A VL S V+L  T  ERP+ P+R+WI  YGLQCL HV  V+
Sbjct: 68  RIDWGYSKPVVAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVW 127

Query: 126 FEYQR-----------SMAHHGF----------EDRAAHRSIMKRLESMNTMTSSVWWVF 185
            EY R           S  H  +          +D +   S +KR ES+NT+ S +WW+ 
Sbjct: 128 SEYWRRNSTRRARDLESYDHEDYNIEYDYEQDSDDNSTTYSFVKRCESINTVISFIWWII 187

Query: 186 GFYWIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIP-IVAFAY 245
           GFYW+V GG  LL ++P LYWL+V+FLA D+FF +FC+ +AC++  +LCCC+P I+A  Y
Sbjct: 188 GFYWVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCIIALLY 247

Query: 246 AMTTREGASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPED 305
           A+   EG SE ++G LP Y F+ A      N+    + +   + ++ +    E  L  ED
Sbjct: 248 AVAGTEGVSEAELGVLPLYKFK-AFHSNEKNITGPGKMV--PIPINGLCLATERTLLAED 307

Query: 306 SECCICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDT 336
           ++CCICLS YEDG EL+ LPCNHHFH  CI KWL++ ATCPLCK NI +G T
Sbjct: 308 ADCCICLSSYEDGAELHALPCNHHFHSTCIVKWLKMRATCPLCKYNILKGTT 356

BLAST of Chy4G084080 vs. ExPASy Swiss-Prot
Match: Q9SRM0 (RING-H2 finger protein ATL66 OS=Arabidopsis thaliana OX=3702 GN=ATL66 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-11
Identity = 26/58 (44.83%), Postives = 41/58 (70.69%), Query Frame = 0

Query: 275 KELALHPEDSECCICLSRYEDGTELYTL-PCNHHFHCGCIAKWLRINATCPLCKSNIR 332
           +E A   E+ ECCICL  +E+G ++  L PC+H +HC C+ +WL+  ++CPLC+ +IR
Sbjct: 96  RERAEEEEEKECCICLGGFEEGEKMKVLPPCSHCYHCECVDRWLKTESSCPLCRVSIR 153

BLAST of Chy4G084080 vs. ExPASy TrEMBL
Match: A0A5D3DQ81 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006360 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 3.9e-193
Identity = 332/337 (98.52%), Postives = 333/337 (98.81%), Query Frame = 0

Query: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDYDDFS 60
           MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSD DDFS
Sbjct: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDSDDFS 60

Query: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120
           RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH
Sbjct: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120

Query: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180
           VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS
Sbjct: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180

Query: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIGTLP 240
           PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDI TLP
Sbjct: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIRTLP 240

Query: 241 KYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCICLSRYEDGTELY 300
           KYTFRQAAVLGTFNLGKEREPI STVE+DN HRIKELALHPEDSECCICLSRYEDGTELY
Sbjct: 241 KYTFRQAAVLGTFNLGKEREPISSTVELDNGHRIKELALHPEDSECCICLSRYEDGTELY 300

Query: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 338
           TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV
Sbjct: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337

BLAST of Chy4G084080 vs. ExPASy TrEMBL
Match: A0A1S3B880 (E3 ubiquitin-protein ligase At4g11680-like OS=Cucumis melo OX=3656 GN=LOC103486858 PE=4 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 3.9e-193
Identity = 332/337 (98.52%), Postives = 333/337 (98.81%), Query Frame = 0

Query: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDYDDFS 60
           MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSD DDFS
Sbjct: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDSDDFS 60

Query: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120
           RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH
Sbjct: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120

Query: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180
           VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS
Sbjct: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180

Query: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIGTLP 240
           PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDI TLP
Sbjct: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIRTLP 240

Query: 241 KYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCICLSRYEDGTELY 300
           KYTFRQAAVLGTFNLGKEREPI STVE+DN HRIKELALHPEDSECCICLSRYEDGTELY
Sbjct: 241 KYTFRQAAVLGTFNLGKEREPISSTVELDNGHRIKELALHPEDSECCICLSRYEDGTELY 300

Query: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 338
           TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV
Sbjct: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337

BLAST of Chy4G084080 vs. ExPASy TrEMBL
Match: A0A0A0LEQ8 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G816170 PE=4 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 3.6e-191
Identity = 330/337 (97.92%), Postives = 332/337 (98.52%), Query Frame = 0

Query: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDYDDFS 60
           MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSD DDFS
Sbjct: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDSDDFS 60

Query: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120
           RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH
Sbjct: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120

Query: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180
           VGFVFFEYQRSMAHHGFEDR AHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS
Sbjct: 121 VGFVFFEYQRSMAHHGFEDRTAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180

Query: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIGTLP 240
           PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDI TLP
Sbjct: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIRTLP 240

Query: 241 KYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCICLSRYEDGTELY 300
           KYTFRQ AVLGTFNLGKEREPIGSTVE+DN HRIKELALHPEDSECCICLSRYEDGTELY
Sbjct: 241 KYTFRQ-AVLGTFNLGKEREPIGSTVELDNSHRIKELALHPEDSECCICLSRYEDGTELY 300

Query: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 338
           TLPCNHHFHCGCIAKWLRINATCPLCKSNIR+GDTLV
Sbjct: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRQGDTLV 336

BLAST of Chy4G084080 vs. ExPASy TrEMBL
Match: A0A6J1J3G3 (E3 ubiquitin-protein ligase At4g11680-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482323 PE=4 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.9e-168
Identity = 298/348 (85.63%), Postives = 312/348 (89.66%), Query Frame = 0

Query: 1   MEEQRALR-----PTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCA----- 60
           MEE+RALR      TLN S+LRYPTVSTTFTIPLTRYTTRLI+S RHRPLLADCA     
Sbjct: 1   MEEERALRRTANGGTLNDSVLRYPTVSTTFTIPLTRYTTRLITSGRHRPLLADCADSRSD 60

Query: 61  -EDHSDYDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLW 120
            EDH D +DF+RRS +RENC YSMPIL+LDVVWNLAFV VS+VVL STFRERPSTPLRLW
Sbjct: 61  TEDHDDANDFNRRSIVRENCFYSMPILILDVVWNLAFVFVSIVVLLSTFRERPSTPLRLW 120

Query: 121 ISGYGLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWI 180
           ISGYGLQCLLHVGFVFFEYQRSMA HGFEDRA HRSIMKRLESMNTMTSSVWWVFGFYWI
Sbjct: 121 ISGYGLQCLLHVGFVFFEYQRSMALHGFEDRAYHRSIMKRLESMNTMTSSVWWVFGFYWI 180

Query: 181 VMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTRE 240
           VMGG ALLQDSPRLYWLTVVFLAFDLFFILFCIGMACV+FFSLCCCIPI+AFAYAMT RE
Sbjct: 181 VMGGHALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVVFFSLCCCIPIIAFAYAMTARE 240

Query: 241 GASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCIC 300
           GASEE I  LPKYTFRQAA LG F  GKEREPIG+T E+DN H IKELALHPEDSECCIC
Sbjct: 241 GASEEVIRALPKYTFRQAA-LGAFIHGKEREPIGATTELDNDHLIKELALHPEDSECCIC 300

Query: 301 LSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 338
           LS+YEDGTELYTLPCNHHFHCGCI KWLRINATCPLCKS+I RGDTLV
Sbjct: 301 LSQYEDGTELYTLPCNHHFHCGCIGKWLRINATCPLCKSSICRGDTLV 347

BLAST of Chy4G084080 vs. ExPASy TrEMBL
Match: A0A6J1F1Q1 (E3 ubiquitin-protein ligase At4g11680-like OS=Cucurbita moschata OX=3662 GN=LOC111441367 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.6e-167
Identity = 297/348 (85.34%), Postives = 311/348 (89.37%), Query Frame = 0

Query: 1   MEEQRALR-----PTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCA----- 60
           MEE+RALR      TLN S+LRYPTVSTTFTIPLTRYTTRLI+S RHRPLLADCA     
Sbjct: 1   MEEERALRRTANGGTLNDSVLRYPTVSTTFTIPLTRYTTRLITSGRHRPLLADCADSRSD 60

Query: 61  -EDHSDYDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLW 120
            EDH D +DF+RRS +RENC YSMPILVLDVVWNLAFVLVS+VVL STFRERPSTPLRLW
Sbjct: 61  TEDHDDANDFNRRSIVRENCFYSMPILVLDVVWNLAFVLVSIVVLLSTFRERPSTPLRLW 120

Query: 121 ISGYGLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWI 180
           ISGYGLQCLLHVGFVFFEYQRSMA HGFEDRA HRSIMKRLESMNTMTSSVWWVFGFYWI
Sbjct: 121 ISGYGLQCLLHVGFVFFEYQRSMALHGFEDRAYHRSIMKRLESMNTMTSSVWWVFGFYWI 180

Query: 181 VMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTRE 240
           VMGG  LLQDSPRLYWLTVVFLAFDLFFILFC+GMACV+FFSLCCCIPI+AFAYAMT RE
Sbjct: 181 VMGGHTLLQDSPRLYWLTVVFLAFDLFFILFCVGMACVVFFSLCCCIPIIAFAYAMTARE 240

Query: 241 GASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCIC 300
           GASEE I  LPKYTFRQAA LG F  GKEREPIG+T E+DN H IKELALHPEDSECCIC
Sbjct: 241 GASEEVIRALPKYTFRQAA-LGAFIHGKEREPIGATTELDNDHLIKELALHPEDSECCIC 300

Query: 301 LSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 338
           LS+YED TELYTLPCNHHFHCGCI KWLRINATCPLCKS+I RGDTLV
Sbjct: 301 LSQYEDRTELYTLPCNHHFHCGCIGKWLRINATCPLCKSSICRGDTLV 347

BLAST of Chy4G084080 vs. NCBI nr
Match: XP_008443208.1 (PREDICTED: E3 ubiquitin-protein ligase At4g11680-like [Cucumis melo] >KAA0053916.1 E3 ubiquitin-protein ligase [Cucumis melo var. makuwa] >TYK25489.1 E3 ubiquitin-protein ligase [Cucumis melo var. makuwa])

HSP 1 Score: 677 bits (1747), Expect = 1.30e-245
Identity = 332/337 (98.52%), Postives = 333/337 (98.81%), Query Frame = 0

Query: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDYDDFS 60
           MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSD DDFS
Sbjct: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDSDDFS 60

Query: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120
           RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH
Sbjct: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120

Query: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180
           VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS
Sbjct: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180

Query: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIGTLP 240
           PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDI TLP
Sbjct: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIRTLP 240

Query: 241 KYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCICLSRYEDGTELY 300
           KYTFRQAAVLGTFNLGKEREPI STVE+DN HRIKELALHPEDSECCICLSRYEDGTELY
Sbjct: 241 KYTFRQAAVLGTFNLGKEREPISSTVELDNGHRIKELALHPEDSECCICLSRYEDGTELY 300

Query: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337
           TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV
Sbjct: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337

BLAST of Chy4G084080 vs. NCBI nr
Match: XP_004136763.1 (E3 ubiquitin-protein ligase At1g12760 [Cucumis sativus] >KGN59404.1 hypothetical protein Csa_002679 [Cucumis sativus])

HSP 1 Score: 671 bits (1730), Expect = 4.90e-243
Identity = 330/337 (97.92%), Postives = 332/337 (98.52%), Query Frame = 0

Query: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDYDDFS 60
           MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSD DDFS
Sbjct: 1   MEEQRALRPTLNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAEDHSDSDDFS 60

Query: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120
           RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH
Sbjct: 61  RRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLH 120

Query: 121 VGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180
           VGFVFFEYQRSMAHHGFEDR AHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS
Sbjct: 121 VGFVFFEYQRSMAHHGFEDRTAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGGQALLQDS 180

Query: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIGTLP 240
           PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDI TLP
Sbjct: 181 PRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASEEDIRTLP 240

Query: 241 KYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCICLSRYEDGTELY 300
           KYTFRQA VLGTFNLGKEREPIGSTVE+DN HRIKELALHPEDSECCICLSRYEDGTELY
Sbjct: 241 KYTFRQA-VLGTFNLGKEREPIGSTVELDNSHRIKELALHPEDSECCICLSRYEDGTELY 300

Query: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337
           TLPCNHHFHCGCIAKWLRINATCPLCKSNIR+GDTLV
Sbjct: 301 TLPCNHHFHCGCIAKWLRINATCPLCKSNIRQGDTLV 336

BLAST of Chy4G084080 vs. NCBI nr
Match: XP_038903580.1 (E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida])

HSP 1 Score: 630 bits (1625), Expect = 6.67e-227
Identity = 314/344 (91.28%), Postives = 324/344 (94.19%), Query Frame = 0

Query: 1   MEEQRALRPT------LNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCAE-DH 60
           MEE+RALR T      LN SLL YPTVSTTFTIPLTRYTTRLI+SDRHRPLLADCAE DH
Sbjct: 1   MEEERALRRTSNGGTLLNRSLLPYPTVSTTFTIPLTRYTTRLITSDRHRPLLADCAEEDH 60

Query: 61  SDYDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGY 120
           SD +DFSRRSFIRENCSYSMPILVLDVVWNLAFVLVS+VVL STF+ERPSTPLRLWISGY
Sbjct: 61  SDSEDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSIVVLLSTFKERPSTPLRLWISGY 120

Query: 121 GLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180
           GLQCLLHV FVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG
Sbjct: 121 GLQCLLHVAFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWIVMGG 180

Query: 181 QALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASE 240
           QALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASE
Sbjct: 181 QALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTREGASE 240

Query: 241 EDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCICLSRY 300
           EDI TLP+YTFRQAA LGT + GKEREPIG+TVE+DN H IKELALHPEDSECCICLSRY
Sbjct: 241 EDIRTLPRYTFRQAAELGTVSRGKEREPIGATVELDNGHCIKELALHPEDSECCICLSRY 300

Query: 301 EDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337
           EDGTELYTLPCNHHFHCGCI+KWLRINATCPLCKSNI RGDTLV
Sbjct: 301 EDGTELYTLPCNHHFHCGCISKWLRINATCPLCKSNICRGDTLV 344

BLAST of Chy4G084080 vs. NCBI nr
Match: XP_022983820.1 (E3 ubiquitin-protein ligase At4g11680-like isoform X1 [Cucurbita maxima] >XP_022983821.1 E3 ubiquitin-protein ligase At4g11680-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 595 bits (1534), Expect = 5.48e-213
Identity = 298/348 (85.63%), Postives = 312/348 (89.66%), Query Frame = 0

Query: 1   MEEQRALRPT-----LNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCA----- 60
           MEE+RALR T     LN S+LRYPTVSTTFTIPLTRYTTRLI+S RHRPLLADCA     
Sbjct: 1   MEEERALRRTANGGTLNDSVLRYPTVSTTFTIPLTRYTTRLITSGRHRPLLADCADSRSD 60

Query: 61  -EDHSDYDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLW 120
            EDH D +DF+RRS +RENC YSMPIL+LDVVWNLAFV VS+VVL STFRERPSTPLRLW
Sbjct: 61  TEDHDDANDFNRRSIVRENCFYSMPILILDVVWNLAFVFVSIVVLLSTFRERPSTPLRLW 120

Query: 121 ISGYGLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWI 180
           ISGYGLQCLLHVGFVFFEYQRSMA HGFEDRA HRSIMKRLESMNTMTSSVWWVFGFYWI
Sbjct: 121 ISGYGLQCLLHVGFVFFEYQRSMALHGFEDRAYHRSIMKRLESMNTMTSSVWWVFGFYWI 180

Query: 181 VMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTRE 240
           VMGG ALLQDSPRLYWLTVVFLAFDLFFILFCIGMACV+FFSLCCCIPI+AFAYAMT RE
Sbjct: 181 VMGGHALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVVFFSLCCCIPIIAFAYAMTARE 240

Query: 241 GASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCIC 300
           GASEE I  LPKYTFRQAA LG F  GKEREPIG+T E+DN H IKELALHPEDSECCIC
Sbjct: 241 GASEEVIRALPKYTFRQAA-LGAFIHGKEREPIGATTELDNDHLIKELALHPEDSECCIC 300

Query: 301 LSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337
           LS+YEDGTELYTLPCNHHFHCGCI KWLRINATCPLCKS+I RGDTLV
Sbjct: 301 LSQYEDGTELYTLPCNHHFHCGCIGKWLRINATCPLCKSSICRGDTLV 347

BLAST of Chy4G084080 vs. NCBI nr
Match: KAG6580862.1 (E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017618.1 E3 ubiquitin protein ligase RIE1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 595 bits (1534), Expect = 5.48e-213
Identity = 298/348 (85.63%), Postives = 312/348 (89.66%), Query Frame = 0

Query: 1   MEEQRALRPT-----LNHSLLRYPTVSTTFTIPLTRYTTRLISSDRHRPLLADCA----- 60
           MEE+RALR T     LN S+LRYPTVSTTFTIPLTRYTTRLI+S RHRPLLADCA     
Sbjct: 1   MEEERALRRTANGGTLNDSVLRYPTVSTTFTIPLTRYTTRLITSGRHRPLLADCADSRSD 60

Query: 61  -EDHSDYDDFSRRSFIRENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLW 120
            EDH D +DF+RRS +RENC YSMPILVLDVVWNLAFVLVS+VVL STFRERPSTPLRLW
Sbjct: 61  TEDHDDANDFNRRSIVRENCFYSMPILVLDVVWNLAFVLVSIVVLLSTFRERPSTPLRLW 120

Query: 121 ISGYGLQCLLHVGFVFFEYQRSMAHHGFEDRAAHRSIMKRLESMNTMTSSVWWVFGFYWI 180
           ISGYGLQCLLHVGFVFFEYQRSMA HGFEDRA HRSIMKRLESMNTMTSSVWWVFGFYWI
Sbjct: 121 ISGYGLQCLLHVGFVFFEYQRSMALHGFEDRAYHRSIMKRLESMNTMTSSVWWVFGFYWI 180

Query: 181 VMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIPIVAFAYAMTTRE 240
           VMGG  LLQDSPRLYWLTVVFLAFDLFFILFC+GMACV+FFSLCCCIPI+AFAYAMT RE
Sbjct: 181 VMGGHTLLQDSPRLYWLTVVFLAFDLFFILFCVGMACVVFFSLCCCIPIIAFAYAMTARE 240

Query: 241 GASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSECCIC 300
           GASEE I  LPKYTFRQAA LG F  GKEREPIG+T E+DN H IKELALHPEDSECCIC
Sbjct: 241 GASEEVIRALPKYTFRQAA-LGAFIHGKEREPIGATTELDNDHLIKELALHPEDSECCIC 300

Query: 301 LSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGDTLV 337
           LS+YEDGTELYTLPCNHHFHCGCI KWLRINATCPLCKS+I RGDTLV
Sbjct: 301 LSQYEDGTELYTLPCNHHFHCGCIGKWLRINATCPLCKSSICRGDTLV 347

BLAST of Chy4G084080 vs. TAIR 10
Match: AT1G68070.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.0e-73
Identity = 137/287 (47.74%), Postives = 185/287 (64.46%), Query Frame = 0

Query: 66  RENCSYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVF 125
           R +  YS P++ LD++WN AFVLV++V+L     E+P+ P+R+WI GY +QCL+HV  V+
Sbjct: 59  RADWGYSKPVVALDMLWNTAFVLVAIVMLLVFKEEKPNVPIRIWICGYAIQCLVHVVLVW 118

Query: 126 FEYQRSMAHHGFEDRAAHRSI---------------MKRLESMNTMTSSVWWVFGFYWIV 185
            E+++  A     D  A ++                 K  ESMNT+ S VWW+ GFYW+V
Sbjct: 119 LEFRKRNARSRPGDLEAAQATNQDSEDEDNDERFLSTKTCESMNTIISFVWWIVGFYWLV 178

Query: 186 MGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIP-IVAFAYAMTTRE 245
            GG  LLQ++  LYWLT VFLAFD+FF +FC+ +AC+I  +LCCC+P I+A  YA+  +E
Sbjct: 179 SGGDILLQNATHLYWLTFVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIALLYAVAGQE 238

Query: 246 GASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVD--NVHRIKELALHPEDSECC 305
           GASE D+  LPKY F         N  K+ +  G  + VD  + +   E  L PED++CC
Sbjct: 239 GASEADLSILPKYRFHTMN-----NDEKQSDGGGKMIPVDAASENLGNERVLLPEDADCC 298

Query: 306 ICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNIRRGD 335
           ICLS YEDG EL +LPCNHHFH  CI KWL++NATCPLCK NI +G+
Sbjct: 299 ICLSSYEDGAELVSLPCNHHFHSTCIVKWLKMNATCPLCKFNILKGN 340

BLAST of Chy4G084080 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.6e-72
Identity = 134/284 (47.18%), Postives = 175/284 (61.62%), Query Frame = 0

Query: 70  SYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQ 129
           +YS P++ LD++WNLAFV + V VL  +  E+P+ PLR+W+ GYG+QC LH+  V  EY+
Sbjct: 104 AYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYR 163

Query: 130 RSMAHH----------------------GFEDRAAHRSIMKRLESMNTMTSSVWWVFGFY 189
           R                             EDR    +  K LES NTM S +WW+ GFY
Sbjct: 164 RRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFY 223

Query: 190 WIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSLCCCIP-IVAFAYAMT 249
           W+  GGQ L  DSP+LYWL ++FL FD+FF++FC+ +ACVI  ++CCC+P I+A  YA+ 
Sbjct: 224 WVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVA 283

Query: 250 TREGASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGSTVEVDNVHRIKELALHPEDSEC 309
            +EGAS+ DI  +PK+ F +   +   + GK R   G   E      I E +L PED+EC
Sbjct: 284 DQEGASKNDIDQMPKFRFTKTGNVEKLS-GKAR---GIMTECGTDSPI-ERSLSPEDAEC 343

Query: 310 CICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSNI 331
           CICL  YEDG EL  LPCNHHFHC CI KWL IN+ CPLCK NI
Sbjct: 344 CICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of Chy4G084080 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.3e-72
Identity = 142/313 (45.37%), Postives = 185/313 (59.11%), Query Frame = 0

Query: 70  SYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQ 129
           +YS P++VLD+VWNLAFV V+  +L  + +E P  PLR+W+ GY LQC+LH+  V  EY+
Sbjct: 95  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 154

Query: 130 R-----------------------------------SMAHHGFEDRA------AHRSIMK 189
           R                                   S  + G +D +         S+ K
Sbjct: 155 RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 214

Query: 190 RLESMNTMTSSVWWVFGFYWIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVI 249
            LES NTM S +WW+ GFYW+  GGQ L Q+SPR+YWL++VFL FD+FF++FC+ +ACVI
Sbjct: 215 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 274

Query: 250 FFSLCCCIP-IVAFAYAMTTREGASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGST-- 309
             ++CCC+P I+A  YA+  +EGAS+EDI  L K+ FR+   LG  N     E  G+T  
Sbjct: 275 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRK---LGDANKHTNDEAQGTTEG 334

Query: 310 --VEVDNVHRIKELALHPEDSECCICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATC 337
              E      I+   L  ED+ECCICLS YEDGTEL  LPC HHFHC C+ KWL INATC
Sbjct: 335 IMTECGTDSPIEHTLLQ-EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATC 394

BLAST of Chy4G084080 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 269.6 bits (688), Expect = 3.3e-72
Identity = 142/313 (45.37%), Postives = 185/313 (59.11%), Query Frame = 0

Query: 70  SYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQ 129
           +YS P++VLD+VWNLAFV V+  +L  + +E P  PLR+W+ GY LQC+LH+  V  EY+
Sbjct: 24  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 83

Query: 130 R-----------------------------------SMAHHGFEDRA------AHRSIMK 189
           R                                   S  + G +D +         S+ K
Sbjct: 84  RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 143

Query: 190 RLESMNTMTSSVWWVFGFYWIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVI 249
            LES NTM S +WW+ GFYW+  GGQ L Q+SPR+YWL++VFL FD+FF++FC+ +ACVI
Sbjct: 144 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 203

Query: 250 FFSLCCCIP-IVAFAYAMTTREGASEEDIGTLPKYTFRQAAVLGTFNLGKEREPIGST-- 309
             ++CCC+P I+A  YA+  +EGAS+EDI  L K+ FR+   LG  N     E  G+T  
Sbjct: 204 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRK---LGDANKHTNDEAQGTTEG 263

Query: 310 --VEVDNVHRIKELALHPEDSECCICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATC 337
              E      I+   L  ED+ECCICLS YEDGTEL  LPC HHFHC C+ KWL INATC
Sbjct: 264 IMTECGTDSPIEHTLLQ-EDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATC 323

BLAST of Chy4G084080 vs. TAIR 10
Match: AT1G63170.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.8e-71
Identity = 139/301 (46.18%), Postives = 178/301 (59.14%), Query Frame = 0

Query: 70  SYSMPILVLDVVWNLAFVLVSVVVLFSTFRERPSTPLRLWISGYGLQCLLHVGFVFFEYQ 129
           +YS P++VLD VWNLAFV+V+  VL  +  E P+ PLR+WI GYGLQC++H+  V  EY+
Sbjct: 69  AYSKPVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYR 128

Query: 130 R--SMAHHGFEDRAA-----------------------------------HRSIMKRLES 189
           R  S        R++                                   + S  K LES
Sbjct: 129 RRNSRRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLES 188

Query: 190 MNTMTSSVWWVFGFYWIVMGGQALLQDSPRLYWLTVVFLAFDLFFILFCIGMACVIFFSL 249
            NTM S +WWV GFYW+  GGQ L Q SP+LYWL +VFL FD+FF++FC+ +ACVI  ++
Sbjct: 189 ANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAV 248

Query: 250 CCCIP-IVAFAYAMTTREGASEEDIGTLPKYTFRQAAVLGTFNLGKE--REPIGSTVEVD 309
           CCC+P I+A  YA+  +EGAS+EDI  L K+ FR+        + +E  +   G  +   
Sbjct: 249 CCCLPCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTEC 308

Query: 310 NVHRIKELALHPEDSECCICLSRYEDGTELYTLPCNHHFHCGCIAKWLRINATCPLCKSN 331
                 E AL  ED+ECCICLS YED TEL  LPC HHFHCGC+ KWL INATCPLCK N
Sbjct: 309 GTDSPVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYN 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z923.6e-7147.18E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q9LN714.7e-7145.37E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q8LDB86.8e-7046.18E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q8GUU22.6e-6944.86E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q9SRM02.5e-1144.83RING-H2 finger protein ATL66 OS=Arabidopsis thaliana OX=3702 GN=ATL66 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DQ813.9e-19398.52E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3B8803.9e-19398.52E3 ubiquitin-protein ligase At4g11680-like OS=Cucumis melo OX=3656 GN=LOC1034868... [more]
A0A0A0LEQ83.6e-19197.92RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G816170 P... [more]
A0A6J1J3G31.9e-16885.63E3 ubiquitin-protein ligase At4g11680-like isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1F1Q11.6e-16785.34E3 ubiquitin-protein ligase At4g11680-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_008443208.11.30e-24598.52PREDICTED: E3 ubiquitin-protein ligase At4g11680-like [Cucumis melo] >KAA0053916... [more]
XP_004136763.14.90e-24397.92E3 ubiquitin-protein ligase At1g12760 [Cucumis sativus] >KGN59404.1 hypothetical... [more]
XP_038903580.16.67e-22791.28E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida][more]
XP_022983820.15.48e-21385.63E3 ubiquitin-protein ligase At4g11680-like isoform X1 [Cucurbita maxima] >XP_022... [more]
KAG6580862.15.48e-21385.63E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
AT1G68070.11.0e-7347.74Zinc finger, C3HC4 type (RING finger) family protein [more]
AT4G11680.12.6e-7247.18Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.13.3e-7245.37Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.23.3e-7245.37Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G63170.14.8e-7146.18Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 286..326
e-value: 4.2E-6
score: 36.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 285..327
e-value: 9.7E-13
score: 48.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 286..327
score: 12.474524
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 264..334
e-value: 9.4E-19
score: 68.4
NoneNo IPR availablePANTHERPTHR45977TARGET OF ERK KINASE MPK-1coord: 15..337
NoneNo IPR availablePANTHERPTHR45977:SF19RING-FINGER UBIQUITIN LIGASEcoord: 15..337
NoneNo IPR availableCDDcd16474RING-H2_RNF111_likecoord: 285..329
e-value: 2.14325E-18
score: 75.8109
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 278..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G084080.1Chy4G084080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane