Chy4G083900 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G083900
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	3'-5' exonuclease domain-containing protein
Location	chrH04: 23110021 .. 23110653 (-)
RNA-Seq Expression	Chy4G083900
Synteny	Chy4G083900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTCTCACCATTTTCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTTCCCTTCTATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACATTGAGTGGCGTCCTAACGTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTTGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAATTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACGATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGGAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGGTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCCTTTGAGATTGGAAGGTATCTGCTGAGTTGA

mRNA sequence

ATGGCCACTCTCACCATTTTCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTTCCCTTCTATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACATTGAGTGGCGTCCTAACGTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTTGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAATTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACGATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGGAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGGTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCCTTTGAGATTGGAAGGTATCTGCTGAGTTGA

Coding sequence (CDS)

ATGGCCACTCTCACCATTTTCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTTCCCTTCTATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACATTGAGTGGCGTCCTAACGTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTTGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAATTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACGATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGGAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGGTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCCTTTGAGATTGGAAGGTATCTGCTGAGTTGA

Protein sequence

MATLTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDIEWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDRQWLNPGQILYATVDAFLSFEIGRYLLS*

Homology

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BLAST of Chy4G083900 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.1e-21
Identity = 60/170 (35.29%), Postives = 95/170 (55.88%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDIEWRPNVDRH-FRNPVATLQLCV-GRRCLIFQLIHASE 100
           M + ++L   R       VGLDIEWRP+  +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 IPQSLIDFLEDDTFTFVGVGIDNDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
           IPQSL   +ED T   VG+GID D +KL+ DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPGQILYATVDAFLSFEIGRYL 209
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ + + L
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

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BLAST of Chy4G083900 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 3.4e-19
Identity = 55/152 (36.18%), Postives = 84/152 (55.26%), Query Frame = 0


Query: 58  VVGLDIEWRPNVDRHFRNPVATLQLCVGR-RCLIFQLIHASEIPQSLIDFLEDDTFTFVG 117
           VVG D+EW P  +R     VA +QLCV   +C +F +   S  PQ L   LE+      G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 118 VGIDNDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL  D+D+ + N V+L ++A  +++  E  T  L +L + +LG+++ K +++
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSI 197

Query: 178 KLSRWDRQWLNPGQILYATVDAFLSFEIGRYL 209
           + S W +  L   Q LYA  DA+  F I R L
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNL 227

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BLAST of Chy4G083900 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-17
Identity = 51/143 (35.66%), Postives = 80/143 (55.94%), Query Frame = 0


Query: 58  VVGLDIEWRPNVDRHFRNPVATLQLCVGR-RCLIFQLIHASEIPQSLIDFLEDDTFTFVG 117
           VVG D+EW P      R+ VA +QLCV   +C +F +   S  PQ L   LE+ +    G
Sbjct: 72  VVGFDMEWPPIYKPGKRSRVAVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAG 131

Query: 118 VGIDNDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL  D+D+ + + V+L ++A ++++  E  T  L  L + VLG+++ K +++
Sbjct: 132 VGIEGDQWKLLRDFDVKLESFVELTDVANEKLKCAE--TWSLNGLVKHVLGKQLLKDKSI 191

Query: 178 KLSRWDRQWLNPGQILYATVDAF 200
           + S W    L   Q LYA  DA+
Sbjct: 192 RCSNWSNFPLTEDQKLYAATDAY 212

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BLAST of Chy4G083900 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 9.3e-17
Identity = 60/177 (33.90%), Postives = 85/177 (48.02%), Query Frame = 0


Query: 29  LTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDIEWRPNVDRHFRNPVATLQLCVGR-R 88
           + ++ +       W+   L+ H +     V+G D EW        R PVA LQL   R  
Sbjct: 52  IEVINSVQDPTTQWVLNELKNHCQTFK--VLGFDCEW--ITVGGSRRPVALLQLSSHRGL 111

Query: 89  CLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDVLKLYDDYDLNVANTVDLRELAADE 148
           C +F+L H  +IPQ L + LEDD+   VGV    D +KL  DY + VA+T+DLR L    
Sbjct: 112 CALFRLCHMKQIPQDLRELLEDDSVIKVGVAPQEDAMKLSHDYGVGVASTLDLRFLCVMA 171

Query: 149 MQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDRQWLNPGQILYATVDAFLSFEI 205
               E    GL  L +  L   +DK   +  S W+ + L P Q+ YA  DA ++  I
Sbjct: 172 GHKPE----GLGKLSKTHLNYTLDKHWRLACSNWEAKTLEPKQLDYAANDALMAVAI 220

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BLAST of Chy4G083900 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 49/152 (32.24%), Postives = 79/152 (51.97%), Query Frame = 0


Query: 58  VVGLDIEWRPNVDRHFRNPVATLQLCVG-RRCLIFQLIHASEIPQSLIDFLEDDTFTFVG 117
           V+G DIEW P   +     VA +Q+CV  ++C +F +   +  P+ L   LED++   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 118 VGIDNDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL  DY+L +   ++L E+A  +++  E  T     L + +   ++ K ++ 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKEKWT--FNGLIKHLFKEQLYKRKSY 191

Query: 178 KLSRWDRQWLNPGQILYATVDAFLSFEIGRYL 209
           + S WD   L   Q LYA  DA+    I + L
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

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BLAST of Chy4G083900 vs. ExPASy TrEMBL
Match: A0A0A0LHC5 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 2.7e-112
Identity = 203/210 (96.67%), Postives = 205/210 (97.62%), Query Frame = 0


Query: 1   MATLTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTI DRQIPLNSHDYYDITFDDDEILTLVTA PSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGID 120
           LD+EWRPN DRHFRNPVATLQLCVGRRCLIFQLIHASE PQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLY+DYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPGQILYATVDAFLSFEIGRYLLS 211
           WDRQWLNP QILYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

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BLAST of Chy4G083900 vs. ExPASy TrEMBL
Match: A0A2I4GID7 (Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.4e-71
Identity = 132/205 (64.39%), Postives = 163/205 (79.51%), Query Frame = 0


Query: 4   LTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDI 63
           ++I+D ++P ++H+ YD++FD D+I TL+T  PS+VD WIS+I RIHRRRLHRLVVGLD+
Sbjct: 3   ISIYDYELPFDTHNLYDVSFDSDQIGTLLTCSPSLVDSWISDIYRIHRRRLHRLVVGLDL 62

Query: 64  EWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+ +RH +NPVATLQLCVGRRCLIFQLIHA  IPQSL+DFL    FTFVGVGI +DV
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHAPYIPQSLVDFLGQTDFTFVGVGIKSDV 122

Query: 124 LKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL DDY L VA  VDLR LA +E+ + + R AGLK L  EVLG+ I+KPRN+ +SRWD 
Sbjct: 123 EKLLDDYGLEVACVVDLRLLAVEELGTTQLRNAGLKQLAWEVLGKRIEKPRNITMSRWDN 182

Query: 184 QWLNPGQILYATVDAFLSFEIGRYL 209
           +WL   Q+ YA +DAFLSFEIGR L
Sbjct: 183 EWLTCAQVQYACLDAFLSFEIGRQL 207

[Close]

BLAST of Chy4G083900 vs. ExPASy TrEMBL
Match: A0A6J1D121 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 PE=4 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 1.2e-70
Identity = 133/197 (67.51%), Postives = 153/197 (77.66%), Query Frame = 0


Query: 14  NSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDIEWRPNVDRHF 73
           NSH+ YDITFD D+I T +T+ P MVD WIS ILR HRRRLHRL+VGLD+EWRPN +R  
Sbjct: 5   NSHNCYDITFDSDQIKTTITSSPYMVDHWISRILRFHRRRLHRLLVGLDVEWRPNFNRSR 64

Query: 74  RNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDVLKLYDDYDLN 133
           RNP ATLQLCVGRRCLIFQLIHA EIP SL+ FL D+TFTFVGVGID D  KL+DDYDL 
Sbjct: 65  RNPAATLQLCVGRRCLIFQLIHAPEIPHSLLHFLGDETFTFVGVGIDQDARKLFDDYDLT 124

Query: 134 VANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDRQWLNPGQILY 193
           V N VDLR +A D     + R AGLK L RE+LGREI KP+ V +SRWD ++L   Q+ Y
Sbjct: 125 VNNAVDLRSMAVDRTGRRDLRNAGLKDLAREILGREIAKPKAVTMSRWDDEYLTSAQVAY 184

Query: 194 ATVDAFLSFEIGRYLLS 211
           A++DAFLSFEIGR L S
Sbjct: 185 ASIDAFLSFEIGRSLSS 201

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BLAST of Chy4G083900 vs. ExPASy TrEMBL
Match: A0A251SDD2 (Putative ribonuclease H-like domain-containing protein OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr15g0495511 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.5e-70
Identity = 129/207 (62.32%), Postives = 165/207 (79.71%), Query Frame = 0


Query: 4   LTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDI 63
           ++I D Q+P N+HDY+D+TF +D ILTLVT  PS VDMWISEI RIHR RLH LVVGLDI
Sbjct: 3   ISISDHQVPFNTHDYFDVTFSNDTILTLVTHTPSFVDMWISEIERIHRHRLHSLVVGLDI 62

Query: 64  EWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+ +R+ +NPVATLQLCVGRRCLIFQL++A  IPQSL++FL +  +TF GVGI++DV
Sbjct: 63  EWRPSFNRNIQNPVATLQLCVGRRCLIFQLLYAPYIPQSLLNFLRNPNYTFTGVGIESDV 122

Query: 124 LKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL +DY+L V    D+R LAA+     EF+ AG+K+L  +VLG+E++KP+ V +S+WD+
Sbjct: 123 EKLVEDYNLGVERMEDVRTLAAEAYGVREFKNAGIKSLAEQVLGKEVNKPKWVTMSKWDQ 182

Query: 184 QWLNPGQILYATVDAFLSFEIGRYLLS 211
            WLNP Q+ YA VDAFLSFEIGR L+S
Sbjct: 183 PWLNPDQVQYACVDAFLSFEIGRVLIS 209

[Close]

BLAST of Chy4G083900 vs. ExPASy TrEMBL
Match: A0A6A1WBZ0 (Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.6e-70
Identity = 132/208 (63.46%), Postives = 164/208 (78.85%), Query Frame = 0


Query: 1   MATLTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVG 60
           MA+++IFD Q+P N+++ Y ++ D D++LTLVT  PSMVD WIS+I RIH RRLHRLVVG
Sbjct: 1   MASISIFDHQLPDNTYNLYVVSLDSDDVLTLVTHSPSMVDSWISDIYRIHSRRLHRLVVG 60

Query: 61  LDIEWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGID 120
           LD+EWRP+ +RH +NPVATLQLCVGRRCLIFQ++ A   P SLI+FL D +FTFVGVGI+
Sbjct: 61  LDVEWRPSFNRHVQNPVATLQLCVGRRCLIFQIVRAPYFPDSLIEFLGDGSFTFVGVGIE 120

Query: 121 NDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           +DV KL DDY+L VAN VDLR+L  +E    E R AGLK L  EVLG++I KPR V LSR
Sbjct: 121 SDVEKLLDDYELEVANVVDLRDLVEEEWGIRELRNAGLKQLAWEVLGKDIQKPRRVTLSR 180

Query: 181 WDRQWLNPGQILYATVDAFLSFEIGRYL 209
           WD + L+  Q+ YA +DAFLSFEIGR+L
Sbjct: 181 WDNERLSHAQVQYACLDAFLSFEIGRFL 208

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BLAST of Chy4G083900 vs. NCBI nr
Match: XP_004136771.1 (Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 417 bits (1073), Expect = 3.98e-147
Identity = 203/210 (96.67%), Postives = 205/210 (97.62%), Query Frame = 0


Query: 1   MATLTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTI DRQIPLNSHDYYDITFDDDEILTLVTA PSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGID 120
           LD+EWRPN DRHFRNPVATLQLCVGRRCLIFQLIHASE PQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLY+DYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPGQILYATVDAFLSFEIGRYLLS 210
           WDRQWLNP QILYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

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BLAST of Chy4G083900 vs. NCBI nr
Match: KAE8651170.1 (hypothetical protein Csa_000755 [Cucumis sativus])

HSP 1 Score: 410 bits (1053), Expect = 7.93e-144
Identity = 199/206 (96.60%), Postives = 201/206 (97.57%), Query Frame = 0


Query: 1   MATLTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTI DRQIPLNSHDYYDITFDDDEILTLVTA PSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGID 120
           LD+EWRPN DRHFRNPVATLQLCVGRRCLIFQLIHASE PQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLY+DYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPGQILYATVDAFLSFEIGR 206
           WDRQWLNP QILYATVDAFLSFEIGR
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGR 206

[Close]

BLAST of Chy4G083900 vs. NCBI nr
Match: XP_038906336.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 363 bits (931), Expect = 1.77e-125
Identity = 176/210 (83.81%), Postives = 192/210 (91.43%), Query Frame = 0


Query: 1   MATLTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVG 60
           MA L+I DRQIPLNSHDYYDITFD+D ILTL+TA  S+VDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MAILSISDRQIPLNSHDYYDITFDNDAILTLLTACLSVVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDIEWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGID 120
           LDIEWRPN +R +R PVATLQLCVGRRCLIFQLIH SEIP+SLIDFLEDDTFTFVGVGID
Sbjct: 61  LDIEWRPNFNRRYRKPVATLQLCVGRRCLIFQLIHTSEIPESLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
            DV +LY+DYDL VANTVDLRELAA+EM+ D+ + AGLK LGREVLGREIDKPRNV LS+
Sbjct: 121 QDVQQLYNDYDLKVANTVDLRELAANEMERDDLKNAGLKILGREVLGREIDKPRNVTLSQ 180

Query: 181 WDRQWLNPGQILYATVDAFLSFEIGRYLLS 210
           WDRQ L+PGQ+LYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQCLSPGQVLYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Chy4G083900 vs. NCBI nr
Match: XP_042939990.1 (Werner Syndrome-like exonuclease [Carya illinoinensis] >KAG2687445.1 hypothetical protein I3760_09G051400 [Carya illinoinensis] >KAG6694513.1 hypothetical protein I3842_09G051700 [Carya illinoinensis])

HSP 1 Score: 283 bits (725), Expect = 4.30e-94
Identity = 132/205 (64.39%), Postives = 165/205 (80.49%), Query Frame = 0


Query: 4   LTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDI 63
           ++IFD ++P ++H+ YD++FD D I TLVT+ PS+VD WI +I RIHRRRL+RL+VGLD+
Sbjct: 3   ISIFDHELPFDTHNLYDVSFDSDRIQTLVTSSPSVVDSWIFDIYRIHRRRLNRLIVGLDL 62

Query: 64  EWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+ +RH +NPVATLQLCVGRRCLIFQLIHA+ IP+SL+DFL    FTFVGVGI +DV
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHATYIPESLVDFLGQTNFTFVGVGIKSDV 122

Query: 124 LKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL DDY+L VA  VDLR LA +E+   + R AGLK L  EVLG++I+KPRN+K+SRWD 
Sbjct: 123 EKLLDDYELEVACVVDLRLLAVEELGKMQLRNAGLKQLAWEVLGKQIEKPRNIKMSRWDN 182

Query: 184 QWLNPGQILYATVDAFLSFEIGRYL 208
           +WL   QI YA +DAFLSFEIGR L
Sbjct: 183 EWLTRAQIQYACLDAFLSFEIGRLL 207

[Close]

BLAST of Chy4G083900 vs. NCBI nr
Match: XP_018843659.1 (Werner Syndrome-like exonuclease [Juglans regia])

HSP 1 Score: 282 bits (721), Expect = 1.74e-93
Identity = 132/205 (64.39%), Postives = 163/205 (79.51%), Query Frame = 0


Query: 4   LTIFDRQIPLNSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDI 63
           ++I+D ++P ++H+ YD++FD D+I TL+T  PS+VD WIS+I RIHRRRLHRLVVGLD+
Sbjct: 3   ISIYDYELPFDTHNLYDVSFDSDQIGTLLTCSPSLVDSWISDIYRIHRRRLHRLVVGLDL 62

Query: 64  EWRPNVDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+ +RH +NPVATLQLCVGRRCLIFQLIHA  IPQSL+DFL    FTFVGVGI +DV
Sbjct: 63  EWRPSFNRHVQNPVATLQLCVGRRCLIFQLIHAPYIPQSLVDFLGQTDFTFVGVGIKSDV 122

Query: 124 LKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL DDY L VA  VDLR LA +E+ + + R AGLK L  EVLG+ I+KPRN+ +SRWD 
Sbjct: 123 EKLLDDYGLEVACVVDLRLLAVEELGTTQLRNAGLKQLAWEVLGKRIEKPRNITMSRWDN 182

Query: 184 QWLNPGQILYATVDAFLSFEIGRYL 208
           +WL   Q+ YA +DAFLSFEIGR L
Sbjct: 183 EWLTCAQVQYACLDAFLSFEIGRQL 207

[Close]

BLAST of Chy4G083900 vs. TAIR 10
Match: AT3G12430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.4e-22
Identity = 67/209 (32.06%), Postives = 98/209 (46.89%), Query Frame = 0


Query: 19  YDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDIEWRPN---------- 78
           Y + F  +E + +VT  PS++  WI ++L  +R   H LVVG+ ++W P+          
Sbjct: 17  YLVDFFGEEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVR 76

Query: 79  ----------------VDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTF 138
                           V R F  P  TLQLCVG RC+I QL H   +PQ L +FL D  +
Sbjct: 77  YRSDSPPVRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDY 136

Query: 139 TFVGVGIDNDVLKL-YDDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGRE-I 198
           TFVG+    D  KL    ++L +A  +DLR+  +D       +    + +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLKRSKHELEIAVLLDLRKFVSDS-SGRTMKRCSFEKIVEENLGHSGV 196

Query: 199 DKPRNVKLSRWDRQWLNPGQILYATVDAF 200
              R V  S W    L   QIL A++D +
Sbjct: 197 RLDRKVSRSDWRVYDLRYVQILQASIDVY 224

[Close]

BLAST of Chy4G083900 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 102.1 bits (253), Expect = 5.8e-22
Identity = 60/170 (35.29%), Postives = 95/170 (55.88%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDIEWRPNVDRH-FRNPVATLQLCV-GRRCLIFQLIHASE 100
           M + ++L   R       VGLDIEWRP+  +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 IPQSLIDFLEDDTFTFVGVGIDNDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
           IPQSL   +ED T   VG+GID D +KL+ DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPGQILYATVDAFLSFEIGRYL 209
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ + + L
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Chy4G083900 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 100.9 bits (250), Expect = 1.3e-21
Identity = 59/166 (35.54%), Postives = 93/166 (56.02%), Query Frame = 0


Query: 41  MWISEILRIHRRRLHRLVVGLDIEWRPNVDRH-FRNPVATLQLCV-GRRCLIFQLIHASE 100
           M + ++L   R       VGLDIEWRP+  +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 IPQSLIDFLEDDTFTFVGVGIDNDVLKLYDDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
           IPQSL   +ED T   VG+GID D +KL+ DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPGQILYATVDAFLSFEI 205
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ +
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHL 274

[Close]

BLAST of Chy4G083900 vs. TAIR 10
Match: AT3G12410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.7e-21
Identity = 66/211 (31.28%), Postives = 101/211 (47.87%), Query Frame = 0


Query: 14  NSHDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHRRRLHRLVVGLDIEW-------- 73
           N+H  Y + F  DE +  VT   S++  WI  +L  +R   H LVVG+ ++W        
Sbjct: 13  NTHLEYSVDFFGDEFIVTVTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYSD 72

Query: 74  -RPN--------VDRHFRNPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVG 133
            RPN        +  +  NP   LQLCVG RCLI QL +  ++P +L  FL D   TFVG
Sbjct: 73  PRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFVG 132

Query: 134 VGIDNDVLKLYD-DYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGRE-IDKPR 193
           V    D  KL    + L +   +D+R    D       R +  + +  E +G + +    
Sbjct: 133 VWNGQDAGKLARCCHQLEIGELLDIRRYVTDSW-GRSMRRSSFEEIVEECMGYQGVMLDP 192

Query: 194 NVKLSRWDRQWLNPGQILYATVDAFLSFEIG 206
            + +S W    L+  QIL A++DA++  ++G
Sbjct: 193 EISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]

BLAST of Chy4G083900 vs. TAIR 10
Match: AT2G36110.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 6.4e-21
Identity = 66/193 (34.20%), Postives = 96/193 (49.74%), Query Frame = 0


Query: 16  HDYYDITFDDDEILTLVTAFPSMVDMWISEILRIHR-RRLHRLVVGLDIEWRPNVDRHFR 75
           H+   I F  + ++  VT   S +  WI  I    R R  H LVVGLD++W P       
Sbjct: 14  HENRYIDFFGERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGS---D 73

Query: 76  NPVATLQLCVGRRCLIFQLIHASEIPQSLIDFLEDDTFTFVGVGIDNDVLKLYD-DYDLN 135
            P   LQLCVG RCLI QL H   IP+ L  FLED+T TFVGV    D  KL    + L 
Sbjct: 74  PPPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLE 133

Query: 136 VANTVDLRELAADEMQSDEFRTAGLKTLGREVLG-REIDKPRNVKLSRWDRQWLNPGQIL 195
           +   +D+R      + +  F     + +  E LG + + K + + +S W  + L+  QI+
Sbjct: 134 IWRLLDIRHYLPTRLLNSSF-----EKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIV 193

Query: 196 YATVDAFLSFEIG 206
            A+ D ++  ++G
Sbjct: 194 QASDDVYVCCKLG 198

The following BLAST results are available for this feature:

BLAST of Chy4G083900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	8.1e-21	35.29	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	3.4e-19	36.18	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	2.4e-17	35.66	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
Q9VGN7	9.3e-17	33.90	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]
O93530	1.3e-15	32.24	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]

BLAST of Chy4G083900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHC5	2.7e-112	96.67	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A2I4GID7	1.4e-71	64.39	Werner Syndrome-like exonuclease OS=Juglans regia OX=51240 GN=LOC109008130 PE=4 ...	[more]
A0A6J1D121	1.2e-70	67.51	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016622 ...	[more]
A0A251SDD2	1.5e-70	62.32	Putative ribonuclease H-like domain-containing protein OS=Helianthus annuus OX=4...	[more]
A0A6A1WBZ0	2.6e-70	63.46	Werner Syndrome-like exonuclease OS=Morella rubra OX=262757 GN=CJ030_MR2G022295 ...	[more]

BLAST of Chy4G083900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136771.1	3.98e-147	96.67	Werner Syndrome-like exonuclease [Cucumis sativus]	[more]
KAE8651170.1	7.93e-144	96.60	hypothetical protein Csa_000755 [Cucumis sativus]	[more]
XP_038906336.1	1.77e-125	83.81	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]
XP_042939990.1	4.30e-94	64.39	Werner Syndrome-like exonuclease [Carya illinoinensis] >KAG2687445.1 hypothetica...	[more]
XP_018843659.1	1.74e-93	64.39	Werner Syndrome-like exonuclease [Juglans regia]	[more]

BLAST of Chy4G083900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12430.1	4.4e-22	32.06	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.2	5.8e-22	35.29	Werner syndrome-like exonuclease	[more]
AT4G13870.1	1.3e-21	35.54	Werner syndrome-like exonuclease	[more]
AT3G12410.1	3.7e-21	31.28	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	6.4e-21	34.20	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 53..208 e-value: 8.9E-13 score: 48.4
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 17..209 e-value: 8.3E-58 score: 197.2
None	No IPR available	PANTHER	PTHR13620:SF65	OS01G0660800 PROTEIN	coord: 10..208
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 10..208
None	No IPR available	CDD	cd06141	WRN_exo	coord: 58..209 e-value: 5.83441E-60 score: 183.549
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 41..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G083900.1	Chy4G083900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding