Chy4G082800 (gene) Cucumber (hystrix) v1

Overview
NameChy4G082800
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionCation/H(+) antiporter 15-like
LocationchrH04: 22219004 .. 22221642 (-)
RNA-Seq ExpressionChy4G082800
SyntenyChy4G082800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCGATGTTAACAGAACCCGACGATCTATCCGCGTTTTTCAGTGGCGGCGGTGGGGGAGCTGGTAGGACTTTGAAGAACATCACGAAAATATGTGCATCTGCTCACCGGATTCATTCCACCGGCGTTTTCACAGGAGTAAACCCTTTGGAATTTTCAGTTCCTCTTCTTTTGTTGCAGGTGGGAATTTGTGCTGGAACTACTCTCTTTTCTTATCAGCTCCTCAAACCCTTCGGTCAACCCCTAATCGTCTCACAAATTTTGGTAACTTCTGTTTTTTTCTTCTTTTCTTCTTTTCTTCTTCTTTTTTTTTTTTTTTTTTTTTTTGTTTCTTCAATTCGATTATCCTTTTGAATTTGAGTTTTGAAATTGCTTTATTGGTTGTGTTGTGATCTTTACCAGAGTGGTTTCGTTCTGAGTTCTTCGGGTTTAGGGCAATGGAATGCATTTAGAGAAACAATTTTTCCCAGCAGGGGATTCGTTTTGTTGGATGTAATGTCTTCAATTGGTGGTATTTTTTACTTCTTTTTGATTGGAGTGCAAACAGATATGATGATTGTGAAGAAGATTGACACAAGAGCATTTGGTATAGGGTATTGTGCTGTGATTGTGCCTTTGGTTCTCACCATCTTTTTCTCTGTAGCTTTGGTGAATGCTTTTGATTCAAAAACTTCCAAAACTATTCTTCTAGTAGGTGGAGTAGAGTCATTTATCAACTTTCCCATGGTTGCTTCTCTTCTTTCTGAGCTTCATTTAATAAACTCTGAGTTTGGGAGAATTGCTTTATCATCTTCCATGGTTTCAGGTATTAGCACCATGTGTATTATAATGATAGGATCTATGTTGGATCCAATAAAAAGAACCACTTATGATGCATTATTTGTGAAATCTGTATCTTGGGTTATTGGAATTGGTCTTGTTTTGTGTAGTTGGTGTGTTATTATGTGGATGATAAAGAAGAATCCAGTGGGGCAGCCATTGAAGGAGGGCTTTGTGATTACATTGCTTTTAGGGGTTTTTGTGAGTGCATTCTGTAGCCAATCTTTGGGTGCTCACAGCTATTTTGGTGCTCTTGTATTCGGGATTATAATACCTCCAGGGCCTCCCATTGGACCAGCAGTGATGGAGAGACTTGAGTCCATCACCTCTTGGATCTTCATGCCTATCTTCTTTTTCAAAACAAGCTTGGTTGTCAATATGCAGTCCATCGAACTAAAGAAACTACTAGGTTTGTCATTCATTATCTTTGTGAGTGCATTTGGAAAGTTCTTTGGTGTCCTTGTGATGTCATTATTCAACAAAATGCCGCTGAGAGATGCTGTGTCGCTTAGCCTCATCATGAATAGTCAAGGAGCTTTCGAGCTTGGTATGTTTAAAATGTTGAAGAAGGACAAGGTAAGAATGAGGTATTTTGTCATTATATATATAATTACATTCAATCATCATGTTTCTCAAACTCCCTGTCGGTTTTGTTTTGTGAATATGTGCAGAAGATAGACAGCGAATCGTTTGGAATTATGTGCACAGGTGTGATGGTTTTGGTTGGGATTATTACTCCCATAATAAGATATTTGTTTGATCCTTCAAGAAGGTATGTAGTTTATAAGAGAAGAACTGTGATGCACTCAAGACCGGAATCTGATCTTCGTGTACTAGTCTGTATTCATGACCAAGAAGATGTTCCAAATGCCATAAACTTACTTGAGGCTTTGAATCCCACAAGACGAAACCATCTTGTTGTGTACATGCTTCATTTTGTCGAGCTTTTTGGTCGAGCTAACCCCCAACTCATTTCACACAAGTTTTCAAGAGGAAGGACTTCAAGGTCCGGCCCTTCCGAGCCTATCATTAATGCATTCAAATACTTTGGACAAAGCAACCGTGAAATTGTTACAATTTATCCCTTCACCGCGATTTCACCTCCAGCATCAATGCATGATGATGTTTATTCACTTGCTCTCGACAAAAGCATTTCCTTGATTCTTGTTCCTTTCCACAAGAGATTTCATTCCAACGGTGTTTTGTCATTATCCAAAAATAAGTTAAAATTGGTCAACAACCATATTCTTGACAAGGCACCTTGCTCGGTCGCTATTGTTGTCAACCGAGGAAATTCAAATATCTTAAGGTCTATTGCAACAGACTTGTATTGTTTTCAAATAGCTGTGGTGTTCTTAGGTGGACCTGACGATCGCGAGGCAATGTTCATCGGGGCAAAAATGTCTGGACATCCCAATATCAACTTGACAGTAATTCGACTGTTGGAGAATGGGAGTGTCACAAGCGATGACATGGAAGAGAGGAGGCTCGACTGTGAGGCAGTGATTGCGTTTCAAAGAGTCATGGTAGACAACTATAGAGTGAGGTTCATAGAAGAAGTGGTGAAGGATGGCAATGGAACAGTCTCTGTACTCCGTTCGATGGGAAACCATTTTGATCTTGTAATGGTTGGGAGACGTCATAACCCCTATTCGGTATTGGTTCAAGGGTTGGTACTTTGGAACGAGCGCACAGAACTTGGAGAAATCGGGGAGGTGTTATCGTCGTCAGATTTCATGGAGAATGCCATGATCTTGGTTGTGCAACAACACACCAATATGGTTCATCAAGAAACTAACTTTGGTTCTCAAGGATAG

mRNA sequence

ATGGGTTCGATGTTAACAGAACCCGACGATCTATCCGCGTTTTTCAGTGGCGGCGGTGGGGGAGCTGGTAGGACTTTGAAGAACATCACGAAAATATGTGCATCTGCTCACCGGATTCATTCCACCGGCGTTTTCACAGGAGTAAACCCTTTGGAATTTTCAGTTCCTCTTCTTTTGTTGCAGGTGGGAATTTGTGCTGGAACTACTCTCTTTTCTTATCAGCTCCTCAAACCCTTCGGTCAACCCCTAATCGTCTCACAAATTTTGAGTGGTTTCGTTCTGAGTTCTTCGGGTTTAGGGCAATGGAATGCATTTAGAGAAACAATTTTTCCCAGCAGGGGATTCGTTTTGTTGGATGTAATGTCTTCAATTGGTGGTATTTTTTACTTCTTTTTGATTGGAGTGCAAACAGATATGATGATTGTGAAGAAGATTGACACAAGAGCATTTGGTATAGGGTATTGTGCTGTGATTGTGCCTTTGGTTCTCACCATCTTTTTCTCTGTAGCTTTGGTGAATGCTTTTGATTCAAAAACTTCCAAAACTATTCTTCTAGTAGGTGGAGTAGAGTCATTTATCAACTTTCCCATGGTTGCTTCTCTTCTTTCTGAGCTTCATTTAATAAACTCTGAGTTTGGGAGAATTGCTTTATCATCTTCCATGGTTTCAGGTATTAGCACCATGTGTATTATAATGATAGGATCTATGTTGGATCCAATAAAAAGAACCACTTATGATGCATTATTTGTGAAATCTGTATCTTGGGTTATTGGAATTGGTCTTGTTTTGTGTAGTTGGTGTGTTATTATGTGGATGATAAAGAAGAATCCAGTGGGGCAGCCATTGAAGGAGGGCTTTGTGATTACATTGCTTTTAGGGGTTTTTGTGAGTGCATTCTGTAGCCAATCTTTGGGTGCTCACAGCTATTTTGGTGCTCTTGTATTCGGGATTATAATACCTCCAGGGCCTCCCATTGGACCAGCAGTGATGGAGAGACTTGAGTCCATCACCTCTTGGATCTTCATGCCTATCTTCTTTTTCAAAACAAGCTTGGTTGTCAATATGCAGTCCATCGAACTAAAGAAACTACTAGGTTTGTCATTCATTATCTTTGTGAGTGCATTTGGAAAGTTCTTTGGTGTCCTTGTGATGTCATTATTCAACAAAATGCCGCTGAGAGATGCTGTGTCGCTTAGCCTCATCATGAATAGTCAAGGAGCTTTCGAGCTTGGTATGTTTAAAATGTTGAAGAAGGACAAGAAGATAGACAGCGAATCGTTTGGAATTATGTGCACAGGTGTGATGGTTTTGGTTGGGATTATTACTCCCATAATAAGATATTTGTTTGATCCTTCAAGAAGGTATGTAGTTTATAAGAGAAGAACTGTGATGCACTCAAGACCGGAATCTGATCTTCGTGTACTAGTCTGTATTCATGACCAAGAAGATGTTCCAAATGCCATAAACTTACTTGAGGCTTTGAATCCCACAAGACGAAACCATCTTGTTGTGTACATGCTTCATTTTGTCGAGCTTTTTGGTCGAGCTAACCCCCAACTCATTTCACACAAGTTTTCAAGAGGAAGGACTTCAAGGTCCGGCCCTTCCGAGCCTATCATTAATGCATTCAAATACTTTGGACAAAGCAACCGTGAAATTGTTACAATTTATCCCTTCACCGCGATTTCACCTCCAGCATCAATGCATGATGATGTTTATTCACTTGCTCTCGACAAAAGCATTTCCTTGATTCTTGTTCCTTTCCACAAGAGATTTCATTCCAACGGTGTTTTGTCATTATCCAAAAATAAGTTAAAATTGGTCAACAACCATATTCTTGACAAGGCACCTTGCTCGGTCGCTATTGTTGTCAACCGAGGAAATTCAAATATCTTAAGGTCTATTGCAACAGACTTGTATTGTTTTCAAATAGCTGTGGTGTTCTTAGGTGGACCTGACGATCGCGAGGCAATGTTCATCGGGGCAAAAATGTCTGGACATCCCAATATCAACTTGACAGTAATTCGACTGTTGGAGAATGGGAGTGTCACAAGCGATGACATGGAAGAGAGGAGGCTCGACTGTGAGGCAGTGATTGCGTTTCAAAGAGTCATGGTAGACAACTATAGAGTGAGGTTCATAGAAGAAGTGGTGAAGGATGGCAATGGAACAGTCTCTGTACTCCGTTCGATGGGAAACCATTTTGATCTTGTAATGGTTGGGAGACGTCATAACCCCTATTCGGTATTGGTTCAAGGGTTGGTACTTTGGAACGAGCGCACAGAACTTGGAGAAATCGGGGAGGTGTTATCGTCGTCAGATTTCATGGAGAATGCCATGATCTTGGTTGTGCAACAACACACCAATATGGTTCATCAAGAAACTAACTTTGGTTCTCAAGGATAG

Coding sequence (CDS)

ATGGGTTCGATGTTAACAGAACCCGACGATCTATCCGCGTTTTTCAGTGGCGGCGGTGGGGGAGCTGGTAGGACTTTGAAGAACATCACGAAAATATGTGCATCTGCTCACCGGATTCATTCCACCGGCGTTTTCACAGGAGTAAACCCTTTGGAATTTTCAGTTCCTCTTCTTTTGTTGCAGGTGGGAATTTGTGCTGGAACTACTCTCTTTTCTTATCAGCTCCTCAAACCCTTCGGTCAACCCCTAATCGTCTCACAAATTTTGAGTGGTTTCGTTCTGAGTTCTTCGGGTTTAGGGCAATGGAATGCATTTAGAGAAACAATTTTTCCCAGCAGGGGATTCGTTTTGTTGGATGTAATGTCTTCAATTGGTGGTATTTTTTACTTCTTTTTGATTGGAGTGCAAACAGATATGATGATTGTGAAGAAGATTGACACAAGAGCATTTGGTATAGGGTATTGTGCTGTGATTGTGCCTTTGGTTCTCACCATCTTTTTCTCTGTAGCTTTGGTGAATGCTTTTGATTCAAAAACTTCCAAAACTATTCTTCTAGTAGGTGGAGTAGAGTCATTTATCAACTTTCCCATGGTTGCTTCTCTTCTTTCTGAGCTTCATTTAATAAACTCTGAGTTTGGGAGAATTGCTTTATCATCTTCCATGGTTTCAGGTATTAGCACCATGTGTATTATAATGATAGGATCTATGTTGGATCCAATAAAAAGAACCACTTATGATGCATTATTTGTGAAATCTGTATCTTGGGTTATTGGAATTGGTCTTGTTTTGTGTAGTTGGTGTGTTATTATGTGGATGATAAAGAAGAATCCAGTGGGGCAGCCATTGAAGGAGGGCTTTGTGATTACATTGCTTTTAGGGGTTTTTGTGAGTGCATTCTGTAGCCAATCTTTGGGTGCTCACAGCTATTTTGGTGCTCTTGTATTCGGGATTATAATACCTCCAGGGCCTCCCATTGGACCAGCAGTGATGGAGAGACTTGAGTCCATCACCTCTTGGATCTTCATGCCTATCTTCTTTTTCAAAACAAGCTTGGTTGTCAATATGCAGTCCATCGAACTAAAGAAACTACTAGGTTTGTCATTCATTATCTTTGTGAGTGCATTTGGAAAGTTCTTTGGTGTCCTTGTGATGTCATTATTCAACAAAATGCCGCTGAGAGATGCTGTGTCGCTTAGCCTCATCATGAATAGTCAAGGAGCTTTCGAGCTTGGTATGTTTAAAATGTTGAAGAAGGACAAGAAGATAGACAGCGAATCGTTTGGAATTATGTGCACAGGTGTGATGGTTTTGGTTGGGATTATTACTCCCATAATAAGATATTTGTTTGATCCTTCAAGAAGGTATGTAGTTTATAAGAGAAGAACTGTGATGCACTCAAGACCGGAATCTGATCTTCGTGTACTAGTCTGTATTCATGACCAAGAAGATGTTCCAAATGCCATAAACTTACTTGAGGCTTTGAATCCCACAAGACGAAACCATCTTGTTGTGTACATGCTTCATTTTGTCGAGCTTTTTGGTCGAGCTAACCCCCAACTCATTTCACACAAGTTTTCAAGAGGAAGGACTTCAAGGTCCGGCCCTTCCGAGCCTATCATTAATGCATTCAAATACTTTGGACAAAGCAACCGTGAAATTGTTACAATTTATCCCTTCACCGCGATTTCACCTCCAGCATCAATGCATGATGATGTTTATTCACTTGCTCTCGACAAAAGCATTTCCTTGATTCTTGTTCCTTTCCACAAGAGATTTCATTCCAACGGTGTTTTGTCATTATCCAAAAATAAGTTAAAATTGGTCAACAACCATATTCTTGACAAGGCACCTTGCTCGGTCGCTATTGTTGTCAACCGAGGAAATTCAAATATCTTAAGGTCTATTGCAACAGACTTGTATTGTTTTCAAATAGCTGTGGTGTTCTTAGGTGGACCTGACGATCGCGAGGCAATGTTCATCGGGGCAAAAATGTCTGGACATCCCAATATCAACTTGACAGTAATTCGACTGTTGGAGAATGGGAGTGTCACAAGCGATGACATGGAAGAGAGGAGGCTCGACTGTGAGGCAGTGATTGCGTTTCAAAGAGTCATGGTAGACAACTATAGAGTGAGGTTCATAGAAGAAGTGGTGAAGGATGGCAATGGAACAGTCTCTGTACTCCGTTCGATGGGAAACCATTTTGATCTTGTAATGGTTGGGAGACGTCATAACCCCTATTCGGTATTGGTTCAAGGGTTGGTACTTTGGAACGAGCGCACAGAACTTGGAGAAATCGGGGAGGTGTTATCGTCGTCAGATTTCATGGAGAATGCCATGATCTTGGTTGTGCAACAACACACCAATATGGTTCATCAAGAAACTAACTTTGGTTCTCAAGGATAG

Protein sequence

MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTSKTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIELKKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHDQEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPIINAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKDGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAMILVVQQHTNMVHQETNFGSQG*
Homology
BLAST of Chy4G082800 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.4e-145
Identity = 298/789 (37.77%), Postives = 481/789 (60.96%), Query Frame = 0

Query: 32  ICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQILSG 91
           IC +   I + GV+ G NPL+FS+PL +LQ+ +    T F   +LKPF QP ++S+IL G
Sbjct: 14  ICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILGG 73

Query: 92  FVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDTRAFG 151
            VL  S LG+   F  TIFP R  ++L+ M+++G +++ FL+GV+ D+M+V+K   RA  
Sbjct: 74  IVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRALT 133

Query: 152 IGYCAVIVPLVLTIFFSVALVNAFDSKTSKT-ILLVGGVESFINFPMVASLLSELHLINS 211
           I    +++P ++   FS ++  + D     T IL +G   S   FP++A +L+EL LIN+
Sbjct: 134 IAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELKLINT 193

Query: 212 EFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDALFVKSVSWVIGIGLVLCSWCV-- 271
           E GRI++S+++V+ +    ++ +   L    +T++ +L+V   S V    + +C + V  
Sbjct: 194 EIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVF---IAVCVFVVRP 253

Query: 272 -IMWMIKKNPVGQPLKEGFVITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIPPGPPIGP 331
            I W+I+K P G+   E  +  +L GV +S F + ++G HS FGA VFG++IP G P+G 
Sbjct: 254 GIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGL 313

Query: 332 AVMERLESITSWIFMPIFFFKTSLVVNMQSIE-LKKLLGLSFIIFVSAFGKFFGVLVMSL 391
            ++E+LE   S + +P+FF  + L  N+ +I+     L L  +IF++  GK  G ++++ 
Sbjct: 314 TLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVAF 373

Query: 392 FNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIITPIIR 451
           F+ MP+R+ ++L L++N++G  E+ +  + K  K +D E+F  M    +V+ G+ITPI+ 
Sbjct: 374 FHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVT 433

Query: 452 YLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHDQEDVPNAINLLEALNPTRRNHLVVYM 511
            L+ P ++ V YKRRT+  ++P+S+LRVLVC+H   +VP  INLLEA +PT+R+ + +Y+
Sbjct: 434 ILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYV 493

Query: 512 LHFVELFGRANPQLISH---KFSRGRTSRS-GPSEPIINAFKYFGQSNREIVTIYPFTAI 571
           LH VEL GRA+  LI H   K  R   +R+   S+ IINAF+ + Q +   V + P TAI
Sbjct: 494 LHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HAAFVAVQPLTAI 553

Query: 572 SPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHILDKAPCSVAI 631
           SP ++MH+DV SLA DK +S I++PFHK+   +G +  +    +LVN ++L+ +PCSV I
Sbjct: 554 SPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGI 613

Query: 632 VVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIRLL--EN 691
           +V+RG +   R + ++    Q+AV+F GGPDDREA+    +M+ HP I LTV+R +  E+
Sbjct: 614 LVDRGLNGATR-LNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDED 673

Query: 692 GSVTS---------------DDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKDGNGTV 751
            + T+               D  ++R+LD + +  F+    +   + +IE++V +G  TV
Sbjct: 674 EADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETV 733

Query: 752 SVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAMILVVQ 795
           + +RSM +  DL +VGR     S L  GL  W+E  ELG IG++L+SSDF     +LVVQ
Sbjct: 734 AAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQ 793

BLAST of Chy4G082800 vs. ExPASy Swiss-Prot
Match: Q9LMJ1 (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 4.2e-110
Identity = 235/765 (30.72%), Postives = 430/765 (56.21%), Query Frame = 0

Query: 32  ICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQILSG 91
           +C   H + S GVF G +PL++++PL+LLQ+ +   T+   Y+LLKP  Q +I +Q+L+G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 92  FVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDTRAFG 151
            +L  S  GQ +A+ +   P  G + L  +S++G   + FL+G++ D  I++K  ++A  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 152 IGYCAVIVPLVLTIFFSVALVNAFD--SKTSKTILLVGGVESFINFPMVASLLSELHLIN 211
           IG  +  +P  L     + L N ++        I  V  + +  +FP+  ++L+EL+++N
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 212 SEFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDALFVKSVSWVIGIGLVLCSWC-- 271
           S+ GR+A + S+V    +  + ++  M   ++  T  +++  S  WV  + LV+   C  
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFRMF--LRDGTLASVW--SFVWVTALILVIFFVCRP 266

Query: 272 VIMWMIKKNPVGQPLKEGFV--ITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIPPGPPI 331
            I+W+ ++  +    K G +    +++ +   +  S+ LG H+ FGA   G+ +P GPP+
Sbjct: 267 AIIWLTERRSISID-KAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPL 326

Query: 332 GPAVMERLESITSWIFMPIFFFKTSLVVNMQSIELKKLLGLSFIIFVSAFGKFFGVLVMS 391
           G  +  +LE   + + +P F   + L  N   I    +  +  +I ++   KF G    S
Sbjct: 327 GTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAAS 386

Query: 392 LFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIITPII 451
            +  + + DA SL+L+M  QG  E+    M K +K +++E F ++   ++++ GI   ++
Sbjct: 387 AYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLV 446

Query: 452 RYLFDPSRRYVVYKRRTVMHSRPES-DLRVLVCIHDQEDVPNAINLLEALNPTRRNHLVV 511
             L+DPS+RY    +RT++ +R  +   R+L+C+++ E+VP+ +NLLEA  P+R + + V
Sbjct: 447 VCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISV 506

Query: 512 YMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPIINAFKYFGQSNREIVTIYPFTAISP 571
           + LH VEL GRA+  L+ H         +  S  I+N F+ F Q N+  +    FTA +P
Sbjct: 507 FTLHLVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAP 566

Query: 572 PASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHILDKAPCSVAIVV 631
            +S++DD+ +LALDK  +LI++PFHK++  +G +      ++ +N ++L+KAPCSV I +
Sbjct: 567 FSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFI 626

Query: 632 NRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIRLLENGSVT 691
           +RG +   RS+        +AV+F+ G DD EA+    +++ HP +++T+I      S+ 
Sbjct: 627 DRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSLQ 686

Query: 692 SD---DMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKDGNGTVSVLRSMGNHFDLVMVG 751
            +   D+E    +   +  F+   +   ++ + EE+V+DG  T  V+ S+G+ FDLV+VG
Sbjct: 687 QNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVVG 746

Query: 752 RRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAMILVVQQ 787
           R H+  S ++ GL  W+E  ELG IG++ +SSDF  + +++  Q+
Sbjct: 747 RDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQE 786

BLAST of Chy4G082800 vs. ExPASy Swiss-Prot
Match: O22920 (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-107
Identity = 237/781 (30.35%), Postives = 443/781 (56.72%), Query Frame = 0

Query: 32  ICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQILSG 91
           +C + + + S G+F   NPL++++PLLLLQ+ +   T+   +++L+P  Q +I +Q+L+G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 92  FVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDTRAFG 151
            VL  S LG    +     P+ G +++  +S++G + + FL+G++ D  I++K  ++A  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 152 IGYCAVIVPLV---LTIFF---SVALVNAFDSKTSKTILLVGGVESFINFPMVASLLSEL 211
           IG  +   P     LTI F   ++ L +   S TS  I L     S  +FP+  ++L+EL
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISL----SSMTSFPVTTTVLAEL 208

Query: 212 HLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDALFVKSVSWVIGIGLVL-- 271
           +++NSE GR+A   SMV  + +  + +  ++    +  T  +L+  ++S +IG+ LV+  
Sbjct: 209 NILNSELGRLATHCSMVCEVCSWFVALAFNLY--TRDRTMTSLY--ALSMIIGLLLVIYF 268

Query: 272 CSWCVIMWMIKKNPVGQPLKE---GFVITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIP 331
               +I+W+ ++       K+    F + LLL   +++   +++G H+ FGA   G+ +P
Sbjct: 269 VFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLS--IASLSGEAMGVHAAFGAFWLGVSLP 328

Query: 332 PGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSI---ELKKLLGLSFIIFVSAFGK 391
            GPP+G  +  +LE   S +F+P F   + L  N   I       ++ +  I+ ++   K
Sbjct: 329 DGPPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCK 388

Query: 392 FFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVL 451
           F G    S + +  + DA+ L+ +M  QG  E+    + K  + +D+E F ++   ++ +
Sbjct: 389 FLGTAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFV 448

Query: 452 VGIITPIIRYLFDPSRRYVVYKRRTVMHSRPES-DLRVLVCIHDQEDVPNAINLLEALNP 511
            GI   ++ YL+DPS+RY    +RT++++R  +  LR+L+ +++ E+VP+ +NLLEA  P
Sbjct: 449 TGISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYP 508

Query: 512 TRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPIINAFKYFGQSNREIVTI 571
           TR N +  + LH VEL GRA+  L  H         +  S  I+NAF+ F Q  +  +  
Sbjct: 509 TRFNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIVNAFQRFEQKYQGALMA 568

Query: 572 YPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHILDKA 631
             FTA +P +S+++D+ +LALDK  +LI++PFHK++  +G +      ++ +N ++LD A
Sbjct: 569 QHFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAA 628

Query: 632 PCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIR 691
           PCSVAI ++RG +   RS+        +A++F+GG DD EA+ +  +M+  P++N+T+I 
Sbjct: 629 PCSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIH 688

Query: 692 LLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKDGNGTVSVLRSMGNHFD 751
                ++  +D  +   +   +  F+    +  ++ ++EE+V+DG  T  V+ S+G+ +D
Sbjct: 689 FRHKSALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYD 748

Query: 752 LVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAMILVVQQHTNMVHQETN 798
           +V+VGR H+  S ++ GL  W+E  ELG IG++L+S DF  + +++  QQ  +++  + +
Sbjct: 749 MVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGDDLLAMDDS 798

BLAST of Chy4G082800 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 9.1e-105
Identity = 237/789 (30.04%), Postives = 420/789 (53.23%), Query Frame = 0

Query: 28  NITKICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQ 87
           N TK C +  +  S GVF G NP++F++PL +LQ+ I    T     LL+P  QP ++++
Sbjct: 4   NSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAE 63

Query: 88  ILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDT 147
           ++ G +L  S LG+  AF + +FP +   +L+ ++++G +F+ FL G++ D   +++   
Sbjct: 64  VIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGK 123

Query: 148 RAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTSKTILLV--GGVESFINFPMVASLLSEL 207
           +A GI    + +P  L I  S  L        + T  LV  G   S   FP++A +L+EL
Sbjct: 124 KALGIALAGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILAEL 183

Query: 208 HLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDAL--FVKSVSWVIGIGLVL 267
            L+ +E GR+A+S++ V+ ++   ++ +   L     +   +L  F+   ++VIG   ++
Sbjct: 184 KLLTTEIGRLAMSAAAVNDVAAWILLALAIALSGSNTSPLVSLWVFLSGCAFVIGASFII 243

Query: 268 CSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIPPGP 327
               +  W+ ++   G+P++E ++   L  V V  F + ++G HS FGA V G++IP   
Sbjct: 244 PP--IFRWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEG 303

Query: 328 PIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIELKKLLGLSFIIFVSA-FGKFFGVL 387
           P   A++E++E + S +F+P++F  + L  N+ +I+  +  GL  ++  +A FGK  G L
Sbjct: 304 PFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILGTL 363

Query: 388 VMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIIT 447
            +SL  K+P+R+A++L  +MN++G  EL +  + K  K ++ ++F IM    +    I T
Sbjct: 364 GVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITT 423

Query: 448 PIIRYLFDPSRRYV---VYKRRTVMHSRPESDLRVLVCIHDQEDVPNAINLLEALNPTRR 507
           P++  ++ P+RR      YK R V      + LR+L C H    +P+ INLLEA     +
Sbjct: 424 PVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEK 483

Query: 508 NH-LVVYMLHFVELFGRANPQLISHKFSR------GRTSRSGPSEPIINAFKYFGQSNRE 567
              L VY LH  EL  R++  L+ HK  +       R   +  ++ ++ AF+ F Q +R 
Sbjct: 484 GEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSR- 543

Query: 568 IVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHI 627
            V + P TAIS  + +H+D+ + A+ K  +++++PFHK    +G L  ++   + VN  +
Sbjct: 544 -VNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRV 603

Query: 628 LDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINL 687
           L +APCSV I V+RG     +  A D+  + + V+F GGPDDREA+  G +M+ HP I L
Sbjct: 604 LLQAPCSVGIFVDRGLGGSSQVSAQDV-SYSVVVLFFGGPDDREALAYGLRMAEHPGIVL 663

Query: 688 TVIRL--------------LENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVV 747
           TV R               + N +  +  ++  + D E +   +++   +  V+F+E+ +
Sbjct: 664 TVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQI 723

Query: 748 KDGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMEN 788
           ++    V          +L +VGR   P   +   +   +E  ELG +G +L S +    
Sbjct: 724 ENAAVDVRSAIEEVRRSNLFLVGRM--PGGEIALAIRENSECPELGPVGSLLISPESSTK 783

BLAST of Chy4G082800 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.0e-104
Identity = 246/760 (32.37%), Postives = 428/760 (56.32%), Query Frame = 0

Query: 71  FSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGF--VLLDVMSSIGGIF 130
           F +  L+  G     S +L+G +LS S L + +A R   F +  +  ++  + ++   + 
Sbjct: 71  FLHFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAARR-FFSTEDYKEIVFSLTAACSYMM 130

Query: 131 YFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVL--TIFF----SVALVNAFDSKTSKT 190
           ++FL+GV+ D  +++    +A  IG  +V++  ++   IFF     V   N+  +  S  
Sbjct: 131 FWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLE 190

Query: 191 ILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPIK- 250
            +++  ++   +FP+V +LL EL L NSE GR+A+SS+++S  ST  +  +   +  +K 
Sbjct: 191 YVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKD 250

Query: 251 -RTTYDALFVKSV----SWVIGIGLVLCSWCV--------IMWMIKKNPVGQPLKEGFVI 310
            +T   ++F+  V      ++  G+V+   C+        + ++IK+ P G+P+K  ++ 
Sbjct: 251 EQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLS 310

Query: 311 TLLLGVFVSA----FCSQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPI 370
           T+++ V  SA    +C QS+    + G  + G+ +P GPP+G A++++ ES     F+P 
Sbjct: 311 TIIVMVSGSAILANWCKQSI----FMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPF 370

Query: 371 FFFKTSLVVNMQSI-ELKKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMN 430
           F   +S  +++ ++   + L G+  I+  S   KF    V +LF  MP+ D  +LSLIM+
Sbjct: 371 FIASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMS 430

Query: 431 SQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTV 490
            +G FELG + +  +   +  E+F + C  + +   II PI+RYL+DPSR Y  Y++R +
Sbjct: 431 FKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNM 490

Query: 491 MHSRPESDLRVLVCIHDQEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISH 550
            H +P S+LR+L CI+  +D+   INLLEA+ P+R + +  Y+LH +EL G+ANP  ISH
Sbjct: 491 QHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISH 550

Query: 551 KFSRGRTSRSGPSEPIINAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISL 610
           K    RT  +  S  ++ +F+ F +     V +  +TA+S P +MH D+  LAL+ + SL
Sbjct: 551 KLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSL 610

Query: 611 ILVPFHKRFHSNGVLSLSKNK-LKLVNNHILDKAPCSVAIVVNR---GNSNI------LR 670
           IL+PFH+ + ++G   +S N  ++ +N  +LD APCSV + V R   G  NI      + 
Sbjct: 611 ILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTIN 670

Query: 671 SIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIRLLENGSVTSDD-MEERR 730
               +L  + I ++FLGG DDREA+ +  +M+  P IN+T++RL+       ++ + ++ 
Sbjct: 671 GTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKM 730

Query: 731 LDCEAVIAFQ-RVMVDNYRVRFIEEVVKDGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLV 790
           LD E +   +   +VD   + + E+ ++D   T S+LRSM + FD+ +VGR +   SV  
Sbjct: 731 LDDELLRDVKSNTLVD---IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFT 790

Query: 791 QGLVLWNERTELGEIGEVLSSSDFMENAMILVVQQHTNMV 792
           +GL  W+E  ELG IG++L+S DF   A +LV+QQ   M+
Sbjct: 791 EGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQQQLMI 822

BLAST of Chy4G082800 vs. ExPASy TrEMBL
Match: A0A0A0LHP9 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824910 PE=4 SV=1)

HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 785/801 (98.00%), Postives = 793/801 (99.00%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTG NPLEFSVPLLLL
Sbjct: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGANPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQW AFRETIFPSRGFVLLDV
Sbjct: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWKAFRETIFPSRGFVLLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           MSSIG IFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPL+LTIFFSVALVNAFDSKTS
Sbjct: 121 MSSIGSIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLLLTIFFSVALVNAFDSKTS 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI
Sbjct: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
           KRTTYDALFV+SVSWVIGIGLVLCS CVIMWMIKKNPVGQPLKEGFV+TLLLGVFVSAFC
Sbjct: 241 KRTTYDALFVESVSWVIGIGLVLCSRCVIMWMIKKNPVGQPLKEGFVVTLLLGVFVSAFC 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL
Sbjct: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDK 420
           KKLLGLSFIIFVSAFGKF  VLVMSLFNKMP+RDAVSLSLIMNSQGAFELGMFKMLKK+K
Sbjct: 361 KKLLGLSFIIFVSAFGKFLSVLVMSLFNKMPVRDAVSLSLIMNSQGAFELGMFKMLKKNK 420

Query: 421 KIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480
           KID+ESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD
Sbjct: 421 KIDNESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480

Query: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPII 540
           QEDVPNAINLLEALNPTRRNHLVVYMLHFV+LFGRANPQLISHKFSRGRTSRSGPSEPII
Sbjct: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVKLFGRANPQLISHKFSRGRTSRSGPSEPII 540

Query: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSL 600
           NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKS+SLILVPFHKRFHSNGVLSL
Sbjct: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSVSLILVPFHKRFHSNGVLSL 600

Query: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660
           SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI
Sbjct: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660

Query: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720
           GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD
Sbjct: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720

Query: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAM 780
           GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 
Sbjct: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAT 780

Query: 781 ILVVQQHTNMVHQETNFGSQG 802
           ILVVQQHTNMVHQET FGSQG
Sbjct: 781 ILVVQQHTNMVHQETIFGSQG 801

BLAST of Chy4G082800 vs. ExPASy TrEMBL
Match: A0A5A7UDC1 (Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001670 PE=4 SV=1)

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 749/795 (94.21%), Postives = 770/795 (96.86%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           MGSMLTEPDDLSAFFSGGGG AGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL
Sbjct: 1   MGSMLTEPDDLSAFFSGGGGEAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           QV  CAGTTLFSYQLLKP GQPLIVSQILSGFVLSSSGLGQW AFRETIFPSRGFVLLDV
Sbjct: 61  QVVFCAGTTLFSYQLLKPLGQPLIVSQILSGFVLSSSGLGQWKAFRETIFPSRGFVLLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLT+ FSVAL NAFDSKTS
Sbjct: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTVLFSVALANAFDSKTS 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCII+IGSMLDPI
Sbjct: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIILIGSMLDPI 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
           KRTTYD LFVKSVSWVI IGLVLCS  VIMWM KKNPVGQPLKEGFV+TLLLGVFVSAFC
Sbjct: 241 KRTTYDRLFVKSVSWVIAIGLVLCSRYVIMWMAKKNPVGQPLKEGFVVTLLLGVFVSAFC 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           SQSLGAHSYFGALV GIIIPPGPPIGPA+MERLESITSW+FMPIFFFKTSLVVNMQSI+L
Sbjct: 301 SQSLGAHSYFGALVLGIIIPPGPPIGPALMERLESITSWMFMPIFFFKTSLVVNMQSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDK 420
           KKLLGLSFIIFVSAFGKF GVLVMSLFNKMP+RDAVSLSLIMNSQGAFELGMFKMLKKDK
Sbjct: 361 KKLLGLSFIIFVSAFGKFLGVLVMSLFNKMPMRDAVSLSLIMNSQGAFELGMFKMLKKDK 420

Query: 421 KIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480
           KID+ESFGIMCTGVMVLVGIITPIIRYLFDPS+RYVVY+RRTVMHSR ESDLRVLVC+HD
Sbjct: 421 KIDNESFGIMCTGVMVLVGIITPIIRYLFDPSKRYVVYRRRTVMHSRSESDLRVLVCVHD 480

Query: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPII 540
           QEDVPNAINLLEALNPTRR+HL+VYMLHFVELFGRANPQLIS KFSRGR+SR GPSE II
Sbjct: 481 QEDVPNAINLLEALNPTRRSHLIVYMLHFVELFGRANPQLISQKFSRGRSSRFGPSESII 540

Query: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSL 600
           NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKS SLILVPFHKRFHSNGVLSL
Sbjct: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSTSLILVPFHKRFHSNGVLSL 600

Query: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660
           SKN LKLVNNHILDKAPCSVAIVVNRGNSN LRSI+TDLYCFQ+A+VFLGGPDDREA+FI
Sbjct: 601 SKNNLKLVNNHILDKAPCSVAIVVNRGNSNTLRSISTDLYCFQVALVFLGGPDDREALFI 660

Query: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720
           GA+MSGHPNINLTVIRL ENG+V SDDMEERRLDCEAVIAFQ VMVDNYRVRFIEEVVKD
Sbjct: 661 GARMSGHPNINLTVIRLSENGNVASDDMEERRLDCEAVIAFQGVMVDNYRVRFIEEVVKD 720

Query: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAM 780
           GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 
Sbjct: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAT 780

Query: 781 ILVVQQHTNMVHQET 796
           ILVVQQHTNMVH+ET
Sbjct: 781 ILVVQQHTNMVHEET 795

BLAST of Chy4G082800 vs. ExPASy TrEMBL
Match: A0A5D3DQ15 (Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007820 PE=4 SV=1)

HSP 1 Score: 1406.7 bits (3640), Expect = 0.0e+00
Identity = 725/795 (91.19%), Postives = 746/795 (93.84%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           MGSMLTEPDDLSAFFSGGGG AGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL
Sbjct: 1   MGSMLTEPDDLSAFFSGGGGEAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           QV                           SGFVLSSSGLGQW AFRETIFPSRGFVLLDV
Sbjct: 61  QV---------------------------SGFVLSSSGLGQWKAFRETIFPSRGFVLLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLT+ FSVAL NAFDSKTS
Sbjct: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTVLFSVALANAFDSKTS 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCII+IGSMLDPI
Sbjct: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIILIGSMLDPI 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
           KRTTYD LFVKSVSWVI IGLVLCS  VIMWM KKNPVGQPLKEGFV+TLLLGVFVSAFC
Sbjct: 241 KRTTYDRLFVKSVSWVIAIGLVLCSRYVIMWMAKKNPVGQPLKEGFVVTLLLGVFVSAFC 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           SQSLGAHSYFGALV GIIIPPGPPIGPA+MERLESITSW+FMPIFFFKTSLVVNMQSI+L
Sbjct: 301 SQSLGAHSYFGALVLGIIIPPGPPIGPALMERLESITSWMFMPIFFFKTSLVVNMQSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDK 420
           KKLLGLSFIIFVSAFGKF GVLVMSLFNKMP+RDAVSLSLIMNSQGAFELGMFKMLKKDK
Sbjct: 361 KKLLGLSFIIFVSAFGKFLGVLVMSLFNKMPMRDAVSLSLIMNSQGAFELGMFKMLKKDK 420

Query: 421 KIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480
           KID+ESFGIMCTGVMVLVGIITPIIRYLFDPS+RYVVY+RRTVMHSR ESDLRVLVC+HD
Sbjct: 421 KIDNESFGIMCTGVMVLVGIITPIIRYLFDPSKRYVVYRRRTVMHSRSESDLRVLVCVHD 480

Query: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPII 540
           QEDVPNAINLLEALNPTRR+HL+VYMLHFVELFGRANPQLIS KFSRGR+SR GPSE II
Sbjct: 481 QEDVPNAINLLEALNPTRRSHLIVYMLHFVELFGRANPQLISQKFSRGRSSRFGPSESII 540

Query: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSL 600
           NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKS SLILVPFHKRFHSNGVLSL
Sbjct: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSTSLILVPFHKRFHSNGVLSL 600

Query: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660
           SKN LKLVNNHILDKAPCSVAIVVNRGNSN LRSI+TDLYCFQ+A+VFLGGPDDREA+FI
Sbjct: 601 SKNNLKLVNNHILDKAPCSVAIVVNRGNSNTLRSISTDLYCFQVALVFLGGPDDREALFI 660

Query: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720
           GA+MSGHPNINLTVIRL ENG+V SDDMEERRLDCEAVIAFQ VMVDNYRVRFIEEVVKD
Sbjct: 661 GARMSGHPNINLTVIRLSENGNVASDDMEERRLDCEAVIAFQGVMVDNYRVRFIEEVVKD 720

Query: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAM 780
           GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 
Sbjct: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAT 768

Query: 781 ILVVQQHTNMVHQET 796
           ILVVQQHTNMVH+ET
Sbjct: 781 ILVVQQHTNMVHEET 768

BLAST of Chy4G082800 vs. ExPASy TrEMBL
Match: A0A6J1JMR6 (cation/H(+) antiporter 15-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486628 PE=4 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 1.2e-293
Identity = 513/797 (64.37%), Postives = 638/797 (80.05%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           M S+L EPDDL+AF    GG A RT  N T IC SAHRIHSTGVF+GVNPLEFSVPLLLL
Sbjct: 1   MSSVLMEPDDLAAFV---GGRASRTSMNFTTICTSAHRIHSTGVFSGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           Q GICAGT +  +QLLKP GQPLIVSQIL G VL SSGL     FR+TIFP RGFV LDV
Sbjct: 61  QFGICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           ++S G IFYFFL+GVQ D+ I+KKID ++ GIG  +VI+PL+LT  +S A +N+ D+KT+
Sbjct: 121 VTSFGTIFYFFLVGVQVDISIMKKIDKKSLGIGTFSVILPLILTSLYSTASMNSVDTKTA 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           K++L+V   ESFINFPMVASLLSELHL+NSEFGRIAL+SSMVS I T C +MIG+++ P 
Sbjct: 181 KSLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGALVAPQ 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
                +++   S+  V+   ++        W+I  NP G+PLKEGFV+ LLLGV ++AF 
Sbjct: 241 NGAVAESMISLSIFIVVSGTIIFIVRQATSWIIMNNPTGEPLKEGFVVALLLGVLITAFS 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           S++ G H YFG L+ G+ IPPGPPIG A++ERL+ ITSW+FMP+FF K  LV+N+ SI+L
Sbjct: 301 SKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMS-LFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKD 420
           K  LG+SF+I+VSA GKF G LV++ ++ +MP+RDAVSL L+M SQGAFELGMFK+++K+
Sbjct: 361 KNFLGVSFVIYVSALGKFLGALVVAVVYCRMPVRDAVSLGLVMASQGAFELGMFKLMRKE 420

Query: 421 KKIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIH 480
            +ID  +F +MC  +MVLV +ITPIIRYLFDPSRRY VYKRRTVM  +P SDLRVL+CIH
Sbjct: 421 GRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPSRRYTVYKRRTVMDLKPGSDLRVLICIH 480

Query: 481 DQEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPI 540
           DQEDVPNAINLLEALNPTRR+HL+VYMLH VEL GRAN QLI H   + R S S PS+ I
Sbjct: 481 DQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSRPSKHI 540

Query: 541 INAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLS 600
           +NAF YFG++NR+ V I+PFT+I+   +MHDDVYSLAL K++SLIL+PFHK+F+SNGV+S
Sbjct: 541 VNAFTYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALKKTVSLILIPFHKKFYSNGVIS 600

Query: 601 LSKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMF 660
           LS   +K+VNN ILDKAPCSV +VV+RG  N+ RS AT+L+ FQ+AVVFLGG DDREAMF
Sbjct: 601 LSNYDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSAATNLHSFQVAVVFLGGADDREAMF 660

Query: 661 IGAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVK 720
           +GA+M+GHPNINLT+IRLL+  +V  DD++E RLD EAV+ F++   +N+RV +IEEVV 
Sbjct: 661 VGARMAGHPNINLTLIRLLKKVNVVRDDVKETRLDSEAVLQFRKATTNNHRVMYIEEVVM 720

Query: 721 DGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 780
           DG GTVSVLRSMGN F+LV+VGRRH+  S+LVQGLVLWNE+TELG IGEVL+SSDFM NA
Sbjct: 721 DGTGTVSVLRSMGNSFNLVIVGRRHSCCSMLVQGLVLWNEQTELGAIGEVLASSDFMGNA 780

Query: 781 MILVVQQHTNMVHQETN 797
           +ILVVQQHT +V++E N
Sbjct: 781 IILVVQQHTKLVNEEQN 794

BLAST of Chy4G082800 vs. ExPASy TrEMBL
Match: A0A6J1EQV6 (cation/H(+) antiporter 15-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435058 PE=4 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 1.4e-289
Identity = 505/797 (63.36%), Postives = 632/797 (79.30%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           M S+L EPDDL+ F    GG A RT  N T +C SAHRIHS GVF+GVNPLEFSVPLLLL
Sbjct: 1   MSSILMEPDDLAVFV---GGSASRTSMNFTTLCTSAHRIHSAGVFSGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           Q GICAGT +  +QLLKP GQPLIVSQIL G VL SSGL     FR+TIFP RGFV LDV
Sbjct: 61  QFGICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           ++S G IFYFFL+GVQ D+ I+K ID ++ GIG  +VI+PL+LT  +S AL+N   ++T+
Sbjct: 121 VTSFGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTA 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           K++L+V   ESFINFPMVASLLSELHL+NSEFGRIALSSSMVS + T C +MIG+++ P 
Sbjct: 181 KSLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALSSSMVSTVWTFCFVMIGALVAPQ 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
                +++   S   ++   ++      + W+IK +P G+PLKEGFV+ +L+GV ++AF 
Sbjct: 241 NGAVAESVISLSTFIMVSGTIIFVVRQAMSWIIKNSPTGEPLKEGFVVAMLIGVLITAFS 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           S++ G H YFG L+ GI IPPGPPIG A++ERL+ ITSW+FMP+FF K  LV+N+ SI+L
Sbjct: 301 SKAFGLHIYFGPLILGISIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSLFN-KMPLRDAVSLSLIMNSQGAFELGMFKMLKKD 420
           K  LG+SF+I+VSA GKF G LV+++ + +MP+RDAVSL L+M SQGAFELGMFK+++K+
Sbjct: 361 KNFLGVSFVIYVSALGKFLGALVVAVVHCRMPVRDAVSLGLVMASQGAFELGMFKLMRKE 420

Query: 421 KKIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIH 480
            +ID  +F +MC  +MVL+ +ITPIIRYLFDPSRRY VYKRRTVM  +  SDLRVL+CIH
Sbjct: 421 GRIDRGTFAVMCIFLMVLMAVITPIIRYLFDPSRRYTVYKRRTVMDLKSGSDLRVLICIH 480

Query: 481 DQEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPI 540
           DQEDVPNAINLLEALNPTRR+HL+VYMLH VEL GRAN QLI H   + R S S PSE I
Sbjct: 481 DQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHI 540

Query: 541 INAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLS 600
           +NAFKYFG++NR+ V I+PFT+I+   +MHDDVY LAL+KS+SLILVPFHK+F+SNGV+S
Sbjct: 541 VNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYLLALNKSVSLILVPFHKKFYSNGVIS 600

Query: 601 LSKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMF 660
           LS   +K+VNN ILDKAPCSV +VV+RG  N+ RS AT+L+ FQ+AVVFLGG DDREAMF
Sbjct: 601 LSNYNMKMVNNEILDKAPCSVGLVVDRGVLNLSRSAATNLHSFQVAVVFLGGADDREAMF 660

Query: 661 IGAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVK 720
           +GA+M+GHP+INLT+IRLL+ G+V  DD++E RLD EAV+ F++   +N RV +IEEVV 
Sbjct: 661 VGARMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVMYIEEVVM 720

Query: 721 DGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 780
           DG GTVS+LRSMGN F LV+VGRRH+  S LVQGLVLWNE+TELG IGEVL+SSDFM NA
Sbjct: 721 DGTGTVSLLRSMGNSFSLVIVGRRHSCCSTLVQGLVLWNEQTELGAIGEVLASSDFMGNA 780

Query: 781 MILVVQQHTNMVHQETN 797
           +ILVVQQHT + ++E N
Sbjct: 781 IILVVQQHTKLANEEQN 794

BLAST of Chy4G082800 vs. NCBI nr
Match: XP_004136799.1 (cation/H(+) antiporter 15 [Cucumis sativus] >KGN59546.1 hypothetical protein Csa_002631 [Cucumis sativus])

HSP 1 Score: 1534 bits (3971), Expect = 0.0
Identity = 785/801 (98.00%), Postives = 793/801 (99.00%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTG NPLEFSVPLLLL
Sbjct: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGANPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQW AFRETIFPSRGFVLLDV
Sbjct: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWKAFRETIFPSRGFVLLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           MSSIG IFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPL+LTIFFSVALVNAFDSKTS
Sbjct: 121 MSSIGSIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLLLTIFFSVALVNAFDSKTS 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI
Sbjct: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
           KRTTYDALFV+SVSWVIGIGLVLCS CVIMWMIKKNPVGQPLKEGFV+TLLLGVFVSAFC
Sbjct: 241 KRTTYDALFVESVSWVIGIGLVLCSRCVIMWMIKKNPVGQPLKEGFVVTLLLGVFVSAFC 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL
Sbjct: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDK 420
           KKLLGLSFIIFVSAFGKF  VLVMSLFNKMP+RDAVSLSLIMNSQGAFELGMFKMLKK+K
Sbjct: 361 KKLLGLSFIIFVSAFGKFLSVLVMSLFNKMPVRDAVSLSLIMNSQGAFELGMFKMLKKNK 420

Query: 421 KIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480
           KID+ESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD
Sbjct: 421 KIDNESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480

Query: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPII 540
           QEDVPNAINLLEALNPTRRNHLVVYMLHFV+LFGRANPQLISHKFSRGRTSRSGPSEPII
Sbjct: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVKLFGRANPQLISHKFSRGRTSRSGPSEPII 540

Query: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSL 600
           NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKS+SLILVPFHKRFHSNGVLSL
Sbjct: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSVSLILVPFHKRFHSNGVLSL 600

Query: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660
           SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI
Sbjct: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660

Query: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720
           GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD
Sbjct: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720

Query: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAM 780
           GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 
Sbjct: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAT 780

Query: 781 ILVVQQHTNMVHQETNFGSQG 801
           ILVVQQHTNMVHQET FGSQG
Sbjct: 781 ILVVQQHTNMVHQETIFGSQG 801

BLAST of Chy4G082800 vs. NCBI nr
Match: KAA0053782.1 (cation/H(+) antiporter 15-like [Cucumis melo var. makuwa])

HSP 1 Score: 1467 bits (3798), Expect = 0.0
Identity = 749/795 (94.21%), Postives = 770/795 (96.86%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           MGSMLTEPDDLSAFFSGGGG AGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL
Sbjct: 1   MGSMLTEPDDLSAFFSGGGGEAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           QV  CAGTTLFSYQLLKP GQPLIVSQILSGFVLSSSGLGQW AFRETIFPSRGFVLLDV
Sbjct: 61  QVVFCAGTTLFSYQLLKPLGQPLIVSQILSGFVLSSSGLGQWKAFRETIFPSRGFVLLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLT+ FSVAL NAFDSKTS
Sbjct: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTVLFSVALANAFDSKTS 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCII+IGSMLDPI
Sbjct: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIILIGSMLDPI 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
           KRTTYD LFVKSVSWVI IGLVLCS  VIMWM KKNPVGQPLKEGFV+TLLLGVFVSAFC
Sbjct: 241 KRTTYDRLFVKSVSWVIAIGLVLCSRYVIMWMAKKNPVGQPLKEGFVVTLLLGVFVSAFC 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           SQSLGAHSYFGALV GIIIPPGPPIGPA+MERLESITSW+FMPIFFFKTSLVVNMQSI+L
Sbjct: 301 SQSLGAHSYFGALVLGIIIPPGPPIGPALMERLESITSWMFMPIFFFKTSLVVNMQSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDK 420
           KKLLGLSFIIFVSAFGKF GVLVMSLFNKMP+RDAVSLSLIMNSQGAFELGMFKMLKKDK
Sbjct: 361 KKLLGLSFIIFVSAFGKFLGVLVMSLFNKMPMRDAVSLSLIMNSQGAFELGMFKMLKKDK 420

Query: 421 KIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480
           KID+ESFGIMCTGVMVLVGIITPIIRYLFDPS+RYVVY+RRTVMHSR ESDLRVLVC+HD
Sbjct: 421 KIDNESFGIMCTGVMVLVGIITPIIRYLFDPSKRYVVYRRRTVMHSRSESDLRVLVCVHD 480

Query: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPII 540
           QEDVPNAINLLEALNPTRR+HL+VYMLHFVELFGRANPQLIS KFSRGR+SR GPSE II
Sbjct: 481 QEDVPNAINLLEALNPTRRSHLIVYMLHFVELFGRANPQLISQKFSRGRSSRFGPSESII 540

Query: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSL 600
           NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKS SLILVPFHKRFHSNGVLSL
Sbjct: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSTSLILVPFHKRFHSNGVLSL 600

Query: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660
           SKN LKLVNNHILDKAPCSVAIVVNRGNSN LRSI+TDLYCFQ+A+VFLGGPDDREA+FI
Sbjct: 601 SKNNLKLVNNHILDKAPCSVAIVVNRGNSNTLRSISTDLYCFQVALVFLGGPDDREALFI 660

Query: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720
           GA+MSGHPNINLTVIRL ENG+V SDDMEERRLDCEAVIAFQ VMVDNYRVRFIEEVVKD
Sbjct: 661 GARMSGHPNINLTVIRLSENGNVASDDMEERRLDCEAVIAFQGVMVDNYRVRFIEEVVKD 720

Query: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAM 780
           GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 
Sbjct: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAT 780

Query: 781 ILVVQQHTNMVHQET 795
           ILVVQQHTNMVH+ET
Sbjct: 781 ILVVQQHTNMVHEET 795

BLAST of Chy4G082800 vs. NCBI nr
Match: TYK25622.1 (cation/H(+) antiporter 15-like [Cucumis melo var. makuwa])

HSP 1 Score: 1407 bits (3641), Expect = 0.0
Identity = 725/795 (91.19%), Postives = 746/795 (93.84%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           MGSMLTEPDDLSAFFSGGGG AGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL
Sbjct: 1   MGSMLTEPDDLSAFFSGGGGEAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           QV                           SGFVLSSSGLGQW AFRETIFPSRGFVLLDV
Sbjct: 61  QV---------------------------SGFVLSSSGLGQWKAFRETIFPSRGFVLLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLT+ FSVAL NAFDSKTS
Sbjct: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTVLFSVALANAFDSKTS 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCII+IGSMLDPI
Sbjct: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIILIGSMLDPI 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
           KRTTYD LFVKSVSWVI IGLVLCS  VIMWM KKNPVGQPLKEGFV+TLLLGVFVSAFC
Sbjct: 241 KRTTYDRLFVKSVSWVIAIGLVLCSRYVIMWMAKKNPVGQPLKEGFVVTLLLGVFVSAFC 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           SQSLGAHSYFGALV GIIIPPGPPIGPA+MERLESITSW+FMPIFFFKTSLVVNMQSI+L
Sbjct: 301 SQSLGAHSYFGALVLGIIIPPGPPIGPALMERLESITSWMFMPIFFFKTSLVVNMQSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDK 420
           KKLLGLSFIIFVSAFGKF GVLVMSLFNKMP+RDAVSLSLIMNSQGAFELGMFKMLKKDK
Sbjct: 361 KKLLGLSFIIFVSAFGKFLGVLVMSLFNKMPMRDAVSLSLIMNSQGAFELGMFKMLKKDK 420

Query: 421 KIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHD 480
           KID+ESFGIMCTGVMVLVGIITPIIRYLFDPS+RYVVY+RRTVMHSR ESDLRVLVC+HD
Sbjct: 421 KIDNESFGIMCTGVMVLVGIITPIIRYLFDPSKRYVVYRRRTVMHSRSESDLRVLVCVHD 480

Query: 481 QEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPII 540
           QEDVPNAINLLEALNPTRR+HL+VYMLHFVELFGRANPQLIS KFSRGR+SR GPSE II
Sbjct: 481 QEDVPNAINLLEALNPTRRSHLIVYMLHFVELFGRANPQLISQKFSRGRSSRFGPSESII 540

Query: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSL 600
           NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKS SLILVPFHKRFHSNGVLSL
Sbjct: 541 NAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSTSLILVPFHKRFHSNGVLSL 600

Query: 601 SKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFI 660
           SKN LKLVNNHILDKAPCSVAIVVNRGNSN LRSI+TDLYCFQ+A+VFLGGPDDREA+FI
Sbjct: 601 SKNNLKLVNNHILDKAPCSVAIVVNRGNSNTLRSISTDLYCFQVALVFLGGPDDREALFI 660

Query: 661 GAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKD 720
           GA+MSGHPNINLTVIRL ENG+V SDDMEERRLDCEAVIAFQ VMVDNYRVRFIEEVVKD
Sbjct: 661 GARMSGHPNINLTVIRLSENGNVASDDMEERRLDCEAVIAFQGVMVDNYRVRFIEEVVKD 720

Query: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAM 780
           GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 
Sbjct: 721 GNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAT 768

Query: 781 ILVVQQHTNMVHQET 795
           ILVVQQHTNMVH+ET
Sbjct: 781 ILVVQQHTNMVHEET 768

BLAST of Chy4G082800 vs. NCBI nr
Match: XP_022989574.1 (cation/H(+) antiporter 15-like isoform X1 [Cucurbita maxima] >XP_022989575.1 cation/H(+) antiporter 15-like isoform X1 [Cucurbita maxima] >XP_022989576.1 cation/H(+) antiporter 15-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1019 bits (2635), Expect = 0.0
Identity = 513/797 (64.37%), Postives = 638/797 (80.05%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           M S+L EPDDL+AF    GG A RT  N T IC SAHRIHSTGVF+GVNPLEFSVPLLLL
Sbjct: 1   MSSVLMEPDDLAAFV---GGRASRTSMNFTTICTSAHRIHSTGVFSGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           Q GICAGT +  +QLLKP GQPLIVSQIL G VL SSGL     FR+TIFP RGFV LDV
Sbjct: 61  QFGICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           ++S G IFYFFL+GVQ D+ I+KKID ++ GIG  +VI+PL+LT  +S A +N+ D+KT+
Sbjct: 121 VTSFGTIFYFFLVGVQVDISIMKKIDKKSLGIGTFSVILPLILTSLYSTASMNSVDTKTA 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           K++L+V   ESFINFPMVASLLSELHL+NSEFGRIAL+SSMVS I T C +MIG+++ P 
Sbjct: 181 KSLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGALVAPQ 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
                +++   S+  V+   ++        W+I  NP G+PLKEGFV+ LLLGV ++AF 
Sbjct: 241 NGAVAESMISLSIFIVVSGTIIFIVRQATSWIIMNNPTGEPLKEGFVVALLLGVLITAFS 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           S++ G H YFG L+ G+ IPPGPPIG A++ERL+ ITSW+FMP+FF K  LV+N+ SI+L
Sbjct: 301 SKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSL-FNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKD 420
           K  LG+SF+I+VSA GKF G LV+++ + +MP+RDAVSL L+M SQGAFELGMFK+++K+
Sbjct: 361 KNFLGVSFVIYVSALGKFLGALVVAVVYCRMPVRDAVSLGLVMASQGAFELGMFKLMRKE 420

Query: 421 KKIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIH 480
            +ID  +F +MC  +MVLV +ITPIIRYLFDPSRRY VYKRRTVM  +P SDLRVL+CIH
Sbjct: 421 GRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPSRRYTVYKRRTVMDLKPGSDLRVLICIH 480

Query: 481 DQEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPI 540
           DQEDVPNAINLLEALNPTRR+HL+VYMLH VEL GRAN QLI H   + R S S PS+ I
Sbjct: 481 DQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSRPSKHI 540

Query: 541 INAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLS 600
           +NAF YFG++NR+ V I+PFT+I+   +MHDDVYSLAL K++SLIL+PFHK+F+SNGV+S
Sbjct: 541 VNAFTYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALKKTVSLILIPFHKKFYSNGVIS 600

Query: 601 LSKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMF 660
           LS   +K+VNN ILDKAPCSV +VV+RG  N+ RS AT+L+ FQ+AVVFLGG DDREAMF
Sbjct: 601 LSNYDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSAATNLHSFQVAVVFLGGADDREAMF 660

Query: 661 IGAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVK 720
           +GA+M+GHPNINLT+IRLL+  +V  DD++E RLD EAV+ F++   +N+RV +IEEVV 
Sbjct: 661 VGARMAGHPNINLTLIRLLKKVNVVRDDVKETRLDSEAVLQFRKATTNNHRVMYIEEVVM 720

Query: 721 DGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 780
           DG GTVSVLRSMGN F+LV+VGRRH+  S+LVQGLVLWNE+TELG IGEVL+SSDFM NA
Sbjct: 721 DGTGTVSVLRSMGNSFNLVIVGRRHSCCSMLVQGLVLWNEQTELGAIGEVLASSDFMGNA 780

Query: 781 MILVVQQHTNMVHQETN 796
           +ILVVQQHT +V++E N
Sbjct: 781 IILVVQQHTKLVNEEQN 794

BLAST of Chy4G082800 vs. NCBI nr
Match: XP_023531603.1 (cation/H(+) antiporter 15-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023531604.1 cation/H(+) antiporter 15-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 508/797 (63.74%), Postives = 633/797 (79.42%), Query Frame = 0

Query: 1   MGSMLTEPDDLSAFFSGGGGGAGRTLKNITKICASAHRIHSTGVFTGVNPLEFSVPLLLL 60
           M S+L EPDDL+ F  G G    RT  N T +C SAHRIHSTGVF+GVNPLEFSVPLLLL
Sbjct: 1   MSSILMEPDDLAVFVGGSGS---RTSMNFTTLCTSAHRIHSTGVFSGVNPLEFSVPLLLL 60

Query: 61  QVGICAGTTLFSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDV 120
           Q GICAGT +  +QLLKP GQPLIVSQIL G VL SSGL     FR+TIFP RGFV LDV
Sbjct: 61  QFGICAGTIILFHQLLKPLGQPLIVSQILGGLVLGSSGLSHMKTFRDTIFPLRGFVFLDV 120

Query: 121 MSSIGGIFYFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTS 180
           ++S G IFYFFL+GVQ D+ I+K ID ++ GIG  +VI+PL+LT  +S AL+N   ++T+
Sbjct: 121 VTSFGTIFYFFLVGVQVDISIMKNIDKKSLGIGTFSVILPLILTSLYSTALMNTVGTRTA 180

Query: 181 KTILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPI 240
           K++L+V   ESFINFPMVASLLSELHL+NSEFGRIAL+SSMVS I T C +MIG+++ P 
Sbjct: 181 KSLLIVSAAESFINFPMVASLLSELHLMNSEFGRIALTSSMVSTIWTACFLMIGTLVAPQ 240

Query: 241 KRTTYDALFVKSVSWVIGIGLVLCSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFC 300
                D++   S   V+   ++        W+IK NP G+PLKEGFV+ LLLGV ++AF 
Sbjct: 241 NGAVADSVISLSTFIVVSGTIIFIVRLATSWIIKNNPAGEPLKEGFVVALLLGVLITAFS 300

Query: 301 SQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIEL 360
           S++ G H YFG L+ G+ IPPGPPIG A++ERL+ ITSW+FMP+FF K  LV+N+ SI+L
Sbjct: 301 SKAFGLHIYFGPLILGVSIPPGPPIGSALVERLDFITSWVFMPVFFVKLGLVINVYSIKL 360

Query: 361 KKLLGLSFIIFVSAFGKFFGVLVMSL-FNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKD 420
           K  LG+SF+I+VSA GKF G L++++ + +MPLRDAVSL L+M SQGAFELGMFK+++K+
Sbjct: 361 KNFLGVSFVIYVSALGKFLGALLVAVVYCRMPLRDAVSLGLVMASQGAFELGMFKLMRKE 420

Query: 421 KKIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIH 480
            +ID  +F +MC  +MVLV +ITPIIRYLFDP+RRY VYKRRTVM  +P SDLRVL+CIH
Sbjct: 421 GRIDRGTFAVMCIFLMVLVAVITPIIRYLFDPTRRYTVYKRRTVMDLKPGSDLRVLICIH 480

Query: 481 DQEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPI 540
           DQEDVPNAINLLEALNPTRR+HL+VYMLH VEL GRAN QLI H   + R S S PSE I
Sbjct: 481 DQEDVPNAINLLEALNPTRRSHLIVYMLHLVELLGRANSQLIPHNLKKDRISTSCPSEHI 540

Query: 541 INAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLS 600
           +NAFKYFG++NR+ V I+PFT+I+   +MHDDVYSLAL+K++SLIL+PFHK+F+SNGV+S
Sbjct: 541 VNAFKYFGRNNRQTVAIFPFTSITSSTTMHDDVYSLALNKTVSLILIPFHKKFYSNGVIS 600

Query: 601 LSKNKLKLVNNHILDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMF 660
           LS   +K+VNN ILDKAPCSV +VV+RG  N+ RS AT+L+ FQ+AVVFLGG DDREAMF
Sbjct: 601 LSNYDMKMVNNEILDKAPCSVGLVVDRGVLNLSRSTATNLHSFQVAVVFLGGADDREAMF 660

Query: 661 IGAKMSGHPNINLTVIRLLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVK 720
           +GA+M+GHP+INLT+IRLL+ G+V  DD++E RLD EAV+ F++   +N RV  IEEVV 
Sbjct: 661 VGARMAGHPDINLTLIRLLKKGNVERDDVKETRLDNEAVLQFRKATTNNRRVMCIEEVVM 720

Query: 721 DGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENA 780
           DG GTVS+LRSMGN FDLV+VGRRH+   +LV+GLVLWNE+TELG IGEVL+SSDFM NA
Sbjct: 721 DGTGTVSLLRSMGNSFDLVIVGRRHSCCPMLVEGLVLWNEQTELGAIGEVLASSDFMGNA 780

Query: 781 MILVVQQHTNMVHQETN 796
           +ILVVQQHT + ++E N
Sbjct: 781 IILVVQQHTKLANEEQN 794

BLAST of Chy4G082800 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 518.5 bits (1334), Expect = 9.8e-147
Identity = 298/789 (37.77%), Postives = 481/789 (60.96%), Query Frame = 0

Query: 32  ICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQILSG 91
           IC +   I + GV+ G NPL+FS+PL +LQ+ +    T F   +LKPF QP ++S+IL G
Sbjct: 14  ICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILGG 73

Query: 92  FVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDTRAFG 151
            VL  S LG+   F  TIFP R  ++L+ M+++G +++ FL+GV+ D+M+V+K   RA  
Sbjct: 74  IVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRALT 133

Query: 152 IGYCAVIVPLVLTIFFSVALVNAFDSKTSKT-ILLVGGVESFINFPMVASLLSELHLINS 211
           I    +++P ++   FS ++  + D     T IL +G   S   FP++A +L+EL LIN+
Sbjct: 134 IAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELKLINT 193

Query: 212 EFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDALFVKSVSWVIGIGLVLCSWCV-- 271
           E GRI++S+++V+ +    ++ +   L    +T++ +L+V   S V    + +C + V  
Sbjct: 194 EIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVF---IAVCVFVVRP 253

Query: 272 -IMWMIKKNPVGQPLKEGFVITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIPPGPPIGP 331
            I W+I+K P G+   E  +  +L GV +S F + ++G HS FGA VFG++IP G P+G 
Sbjct: 254 GIAWIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGL 313

Query: 332 AVMERLESITSWIFMPIFFFKTSLVVNMQSIE-LKKLLGLSFIIFVSAFGKFFGVLVMSL 391
            ++E+LE   S + +P+FF  + L  N+ +I+     L L  +IF++  GK  G ++++ 
Sbjct: 314 TLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVAF 373

Query: 392 FNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIITPIIR 451
           F+ MP+R+ ++L L++N++G  E+ +  + K  K +D E+F  M    +V+ G+ITPI+ 
Sbjct: 374 FHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVT 433

Query: 452 YLFDPSRRYVVYKRRTVMHSRPESDLRVLVCIHDQEDVPNAINLLEALNPTRRNHLVVYM 511
            L+ P ++ V YKRRT+  ++P+S+LRVLVC+H   +VP  INLLEA +PT+R+ + +Y+
Sbjct: 434 ILYKPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYV 493

Query: 512 LHFVELFGRANPQLISH---KFSRGRTSRS-GPSEPIINAFKYFGQSNREIVTIYPFTAI 571
           LH VEL GRA+  LI H   K  R   +R+   S+ IINAF+ + Q +   V + P TAI
Sbjct: 494 LHLVELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HAAFVAVQPLTAI 553

Query: 572 SPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHILDKAPCSVAI 631
           SP ++MH+DV SLA DK +S I++PFHK+   +G +  +    +LVN ++L+ +PCSV I
Sbjct: 554 SPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGI 613

Query: 632 VVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIRLL--EN 691
           +V+RG +   R + ++    Q+AV+F GGPDDREA+    +M+ HP I LTV+R +  E+
Sbjct: 614 LVDRGLNGATR-LNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDED 673

Query: 692 GSVTS---------------DDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKDGNGTV 751
            + T+               D  ++R+LD + +  F+    +   + +IE++V +G  TV
Sbjct: 674 EADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETV 733

Query: 752 SVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAMILVVQ 795
           + +RSM +  DL +VGR     S L  GL  W+E  ELG IG++L+SSDF     +LVVQ
Sbjct: 734 AAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQ 793

BLAST of Chy4G082800 vs. TAIR 10
Match: AT1G06970.1 (cation/hydrogen exchanger 14 )

HSP 1 Score: 400.6 bits (1028), Expect = 3.0e-111
Identity = 235/765 (30.72%), Postives = 430/765 (56.21%), Query Frame = 0

Query: 32  ICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQILSG 91
           +C   H + S GVF G +PL++++PL+LLQ+ +   T+   Y+LLKP  Q +I +Q+L+G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 92  FVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDTRAFG 151
            +L  S  GQ +A+ +   P  G + L  +S++G   + FL+G++ D  I++K  ++A  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 152 IGYCAVIVPLVLTIFFSVALVNAFD--SKTSKTILLVGGVESFINFPMVASLLSELHLIN 211
           IG  +  +P  L     + L N ++        I  V  + +  +FP+  ++L+EL+++N
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 212 SEFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDALFVKSVSWVIGIGLVLCSWC-- 271
           S+ GR+A + S+V    +  + ++  M   ++  T  +++  S  WV  + LV+   C  
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFRMF--LRDGTLASVW--SFVWVTALILVIFFVCRP 266

Query: 272 VIMWMIKKNPVGQPLKEGFV--ITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIPPGPPI 331
            I+W+ ++  +    K G +    +++ +   +  S+ LG H+ FGA   G+ +P GPP+
Sbjct: 267 AIIWLTERRSISID-KAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPL 326

Query: 332 GPAVMERLESITSWIFMPIFFFKTSLVVNMQSIELKKLLGLSFIIFVSAFGKFFGVLVMS 391
           G  +  +LE   + + +P F   + L  N   I    +  +  +I ++   KF G    S
Sbjct: 327 GTGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAAS 386

Query: 392 LFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIITPII 451
            +  + + DA SL+L+M  QG  E+    M K +K +++E F ++   ++++ GI   ++
Sbjct: 387 AYCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLV 446

Query: 452 RYLFDPSRRYVVYKRRTVMHSRPES-DLRVLVCIHDQEDVPNAINLLEALNPTRRNHLVV 511
             L+DPS+RY    +RT++ +R  +   R+L+C+++ E+VP+ +NLLEA  P+R + + V
Sbjct: 447 VCLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISV 506

Query: 512 YMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPIINAFKYFGQSNREIVTIYPFTAISP 571
           + LH VEL GRA+  L+ H         +  S  I+N F+ F Q N+  +    FTA +P
Sbjct: 507 FTLHLVELKGRAHAVLVPHHQMNKLDPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAP 566

Query: 572 PASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHILDKAPCSVAIVV 631
            +S++DD+ +LALDK  +LI++PFHK++  +G +      ++ +N ++L+KAPCSV I +
Sbjct: 567 FSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFI 626

Query: 632 NRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIRLLENGSVT 691
           +RG +   RS+        +AV+F+ G DD EA+    +++ HP +++T+I      S+ 
Sbjct: 627 DRGETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSLQ 686

Query: 692 SD---DMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKDGNGTVSVLRSMGNHFDLVMVG 751
            +   D+E    +   +  F+   +   ++ + EE+V+DG  T  V+ S+G+ FDLV+VG
Sbjct: 687 QNHVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVVG 746

Query: 752 RRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAMILVVQQ 787
           R H+  S ++ GL  W+E  ELG IG++ +SSDF  + +++  Q+
Sbjct: 747 RDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQE 786

BLAST of Chy4G082800 vs. TAIR 10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 392.5 bits (1007), Expect = 8.1e-109
Identity = 237/781 (30.35%), Postives = 443/781 (56.72%), Query Frame = 0

Query: 32  ICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQILSG 91
           +C + + + S G+F   NPL++++PLLLLQ+ +   T+   +++L+P  Q +I +Q+L+G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 92  FVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDTRAFG 151
            VL  S LG    +     P+ G +++  +S++G + + FL+G++ D  I++K  ++A  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 152 IGYCAVIVPLV---LTIFF---SVALVNAFDSKTSKTILLVGGVESFINFPMVASLLSEL 211
           IG  +   P     LTI F   ++ L +   S TS  I L     S  +FP+  ++L+EL
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISL----SSMTSFPVTTTVLAEL 208

Query: 212 HLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDALFVKSVSWVIGIGLVL-- 271
           +++NSE GR+A   SMV  + +  + +  ++    +  T  +L+  ++S +IG+ LV+  
Sbjct: 209 NILNSELGRLATHCSMVCEVCSWFVALAFNLY--TRDRTMTSLY--ALSMIIGLLLVIYF 268

Query: 272 CSWCVIMWMIKKNPVGQPLKE---GFVITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIP 331
               +I+W+ ++       K+    F + LLL   +++   +++G H+ FGA   G+ +P
Sbjct: 269 VFRPIIVWLTQRKTKSMDKKDVVPFFPVLLLLS--IASLSGEAMGVHAAFGAFWLGVSLP 328

Query: 332 PGPPIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSI---ELKKLLGLSFIIFVSAFGK 391
            GPP+G  +  +LE   S +F+P F   + L  N   I       ++ +  I+ ++   K
Sbjct: 329 DGPPLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCK 388

Query: 392 FFGVLVMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVL 451
           F G    S + +  + DA+ L+ +M  QG  E+    + K  + +D+E F ++   ++ +
Sbjct: 389 FLGTAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFV 448

Query: 452 VGIITPIIRYLFDPSRRYVVYKRRTVMHSRPES-DLRVLVCIHDQEDVPNAINLLEALNP 511
            GI   ++ YL+DPS+RY    +RT++++R  +  LR+L+ +++ E+VP+ +NLLEA  P
Sbjct: 449 TGISRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYP 508

Query: 512 TRRNHLVVYMLHFVELFGRANPQLISHKFSRGRTSRSGPSEPIINAFKYFGQSNREIVTI 571
           TR N +  + LH VEL GRA+  L  H         +  S  I+NAF+ F Q  +  +  
Sbjct: 509 TRFNPISFFTLHLVELKGRAHALLTPHHQMNKLDPNTAQSTHIVNAFQRFEQKYQGALMA 568

Query: 572 YPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHILDKA 631
             FTA +P +S+++D+ +LALDK  +LI++PFHK++  +G +      ++ +N ++LD A
Sbjct: 569 QHFTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAA 628

Query: 632 PCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIR 691
           PCSVAI ++RG +   RS+        +A++F+GG DD EA+ +  +M+  P++N+T+I 
Sbjct: 629 PCSVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIH 688

Query: 692 LLENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVVKDGNGTVSVLRSMGNHFD 751
                ++  +D  +   +   +  F+    +  ++ ++EE+V+DG  T  V+ S+G+ +D
Sbjct: 689 FRHKSALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYD 748

Query: 752 LVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMENAMILVVQQHTNMVHQETN 798
           +V+VGR H+  S ++ GL  W+E  ELG IG++L+S DF  + +++  QQ  +++  + +
Sbjct: 749 MVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGDDLLAMDDS 798

BLAST of Chy4G082800 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 382.9 bits (982), Expect = 6.4e-106
Identity = 237/789 (30.04%), Postives = 420/789 (53.23%), Query Frame = 0

Query: 28  NITKICASAHRIHSTGVFTGVNPLEFSVPLLLLQVGICAGTTLFSYQLLKPFGQPLIVSQ 87
           N TK C +  +  S GVF G NP++F++PL +LQ+ I    T     LL+P  QP ++++
Sbjct: 4   NSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAE 63

Query: 88  ILSGFVLSSSGLGQWNAFRETIFPSRGFVLLDVMSSIGGIFYFFLIGVQTDMMIVKKIDT 147
           ++ G +L  S LG+  AF + +FP +   +L+ ++++G +F+ FL G++ D   +++   
Sbjct: 64  VIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGK 123

Query: 148 RAFGIGYCAVIVPLVLTIFFSVALVNAFDSKTSKTILLV--GGVESFINFPMVASLLSEL 207
           +A GI    + +P  L I  S  L        + T  LV  G   S   FP++A +L+EL
Sbjct: 124 KALGIALAGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILAEL 183

Query: 208 HLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPIKRTTYDAL--FVKSVSWVIGIGLVL 267
            L+ +E GR+A+S++ V+ ++   ++ +   L     +   +L  F+   ++VIG   ++
Sbjct: 184 KLLTTEIGRLAMSAAAVNDVAAWILLALAIALSGSNTSPLVSLWVFLSGCAFVIGASFII 243

Query: 268 CSWCVIMWMIKKNPVGQPLKEGFVITLLLGVFVSAFCSQSLGAHSYFGALVFGIIIPPGP 327
               +  W+ ++   G+P++E ++   L  V V  F + ++G HS FGA V G++IP   
Sbjct: 244 PP--IFRWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEG 303

Query: 328 PIGPAVMERLESITSWIFMPIFFFKTSLVVNMQSIELKKLLGLSFIIFVSA-FGKFFGVL 387
           P   A++E++E + S +F+P++F  + L  N+ +I+  +  GL  ++  +A FGK  G L
Sbjct: 304 PFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILGTL 363

Query: 388 VMSLFNKMPLRDAVSLSLIMNSQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIIT 447
            +SL  K+P+R+A++L  +MN++G  EL +  + K  K ++ ++F IM    +    I T
Sbjct: 364 GVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITT 423

Query: 448 PIIRYLFDPSRRYV---VYKRRTVMHSRPESDLRVLVCIHDQEDVPNAINLLEALNPTRR 507
           P++  ++ P+RR      YK R V      + LR+L C H    +P+ INLLEA     +
Sbjct: 424 PVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEK 483

Query: 508 NH-LVVYMLHFVELFGRANPQLISHKFSR------GRTSRSGPSEPIINAFKYFGQSNRE 567
              L VY LH  EL  R++  L+ HK  +       R   +  ++ ++ AF+ F Q +R 
Sbjct: 484 GEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSR- 543

Query: 568 IVTIYPFTAISPPASMHDDVYSLALDKSISLILVPFHKRFHSNGVLSLSKNKLKLVNNHI 627
            V + P TAIS  + +H+D+ + A+ K  +++++PFHK    +G L  ++   + VN  +
Sbjct: 544 -VNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRV 603

Query: 628 LDKAPCSVAIVVNRGNSNILRSIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINL 687
           L +APCSV I V+RG     +  A D+  + + V+F GGPDDREA+  G +M+ HP I L
Sbjct: 604 LLQAPCSVGIFVDRGLGGSSQVSAQDV-SYSVVVLFFGGPDDREALAYGLRMAEHPGIVL 663

Query: 688 TVIRL--------------LENGSVTSDDMEERRLDCEAVIAFQRVMVDNYRVRFIEEVV 747
           TV R               + N +  +  ++  + D E +   +++   +  V+F+E+ +
Sbjct: 664 TVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQI 723

Query: 748 KDGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLVQGLVLWNERTELGEIGEVLSSSDFMEN 788
           ++    V          +L +VGR   P   +   +   +E  ELG +G +L S +    
Sbjct: 724 ENAAVDVRSAIEEVRRSNLFLVGRM--PGGEIALAIRENSECPELGPVGSLLISPESSTK 783

BLAST of Chy4G082800 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 381.7 bits (979), Expect = 1.4e-105
Identity = 246/760 (32.37%), Postives = 428/760 (56.32%), Query Frame = 0

Query: 71  FSYQLLKPFGQPLIVSQILSGFVLSSSGLGQWNAFRETIFPSRGF--VLLDVMSSIGGIF 130
           F +  L+  G     S +L+G +LS S L + +A R   F +  +  ++  + ++   + 
Sbjct: 71  FLHFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAARR-FFSTEDYKEIVFSLTAACSYMM 130

Query: 131 YFFLIGVQTDMMIVKKIDTRAFGIGYCAVIVPLVL--TIFF----SVALVNAFDSKTSKT 190
           ++FL+GV+ D  +++    +A  IG  +V++  ++   IFF     V   N+  +  S  
Sbjct: 131 FWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLE 190

Query: 191 ILLVGGVESFINFPMVASLLSELHLINSEFGRIALSSSMVSGISTMCIIMIGSMLDPIK- 250
            +++  ++   +FP+V +LL EL L NSE GR+A+SS+++S  ST  +  +   +  +K 
Sbjct: 191 YVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKD 250

Query: 251 -RTTYDALFVKSV----SWVIGIGLVLCSWCV--------IMWMIKKNPVGQPLKEGFVI 310
            +T   ++F+  V      ++  G+V+   C+        + ++IK+ P G+P+K  ++ 
Sbjct: 251 EQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLS 310

Query: 311 TLLLGVFVSA----FCSQSLGAHSYFGALVFGIIIPPGPPIGPAVMERLESITSWIFMPI 370
           T+++ V  SA    +C QS+    + G  + G+ +P GPP+G A++++ ES     F+P 
Sbjct: 311 TIIVMVSGSAILANWCKQSI----FMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPF 370

Query: 371 FFFKTSLVVNMQSI-ELKKLLGLSFIIFVSAFGKFFGVLVMSLFNKMPLRDAVSLSLIMN 430
           F   +S  +++ ++   + L G+  I+  S   KF    V +LF  MP+ D  +LSLIM+
Sbjct: 371 FIASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMS 430

Query: 431 SQGAFELGMFKMLKKDKKIDSESFGIMCTGVMVLVGIITPIIRYLFDPSRRYVVYKRRTV 490
            +G FELG + +  +   +  E+F + C  + +   II PI+RYL+DPSR Y  Y++R +
Sbjct: 431 FKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNM 490

Query: 491 MHSRPESDLRVLVCIHDQEDVPNAINLLEALNPTRRNHLVVYMLHFVELFGRANPQLISH 550
            H +P S+LR+L CI+  +D+   INLLEA+ P+R + +  Y+LH +EL G+ANP  ISH
Sbjct: 491 QHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISH 550

Query: 551 KFSRGRTSRSGPSEPIINAFKYFGQSNREIVTIYPFTAISPPASMHDDVYSLALDKSISL 610
           K    RT  +  S  ++ +F+ F +     V +  +TA+S P +MH D+  LAL+ + SL
Sbjct: 551 KLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSL 610

Query: 611 ILVPFHKRFHSNGVLSLSKNK-LKLVNNHILDKAPCSVAIVVNR---GNSNI------LR 670
           IL+PFH+ + ++G   +S N  ++ +N  +LD APCSV + V R   G  NI      + 
Sbjct: 611 ILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTIN 670

Query: 671 SIATDLYCFQIAVVFLGGPDDREAMFIGAKMSGHPNINLTVIRLLENGSVTSDD-MEERR 730
               +L  + I ++FLGG DDREA+ +  +M+  P IN+T++RL+       ++ + ++ 
Sbjct: 671 GTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKM 730

Query: 731 LDCEAVIAFQ-RVMVDNYRVRFIEEVVKDGNGTVSVLRSMGNHFDLVMVGRRHNPYSVLV 790
           LD E +   +   +VD   + + E+ ++D   T S+LRSM + FD+ +VGR +   SV  
Sbjct: 731 LDDELLRDVKSNTLVD---IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFT 790

Query: 791 QGLVLWNERTELGEIGEVLSSSDFMENAMILVVQQHTNMV 792
           +GL  W+E  ELG IG++L+S DF   A +LV+QQ   M+
Sbjct: 791 EGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQQQLMI 822

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIT51.4e-14537.77Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
Q9LMJ14.2e-11030.72Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1[more]
O229201.1e-10730.35Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1[more]
Q9FFR99.1e-10530.04Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1[more]
Q9FFB82.0e-10432.37Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LHP90.0e+0098.00Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824... [more]
A0A5A7UDC10.0e+0094.21Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A5D3DQ150.0e+0091.19Cation/H(+) antiporter 15-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1JMR61.2e-29364.37cation/H(+) antiporter 15-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1EQV61.4e-28963.36cation/H(+) antiporter 15-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_004136799.10.098.00cation/H(+) antiporter 15 [Cucumis sativus] >KGN59546.1 hypothetical protein Csa... [more]
KAA0053782.10.094.21cation/H(+) antiporter 15-like [Cucumis melo var. makuwa][more]
TYK25622.10.091.19cation/H(+) antiporter 15-like [Cucumis melo var. makuwa][more]
XP_022989574.10.064.37cation/H(+) antiporter 15-like isoform X1 [Cucurbita maxima] >XP_022989575.1 cat... [more]
XP_023531603.10.063.74cation/H(+) antiporter 15-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02353... [more]
Match NameE-valueIdentityDescription
AT2G13620.19.8e-14737.77cation/hydrogen exchanger 15 [more]
AT1G06970.13.0e-11130.72cation/hydrogen exchanger 14 [more]
AT2G30240.18.1e-10930.35Cation/hydrogen exchanger family protein [more]
AT5G41610.16.4e-10630.04cation/H+ exchanger 18 [more]
AT5G22900.11.4e-10532.37cation/H+ exchanger 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 67..446
e-value: 2.3E-29
score: 102.3
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 57..455
e-value: 1.9E-51
score: 177.1
NoneNo IPR availablePANTHERPTHR32468:SF114CATION/H(+) ANTIPORTER 15-LIKE ISOFORM X1coord: 21..795
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 21..795

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G082800.1Chy4G082800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006885 regulation of pH
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0012505 endomembrane system
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity