Chy4G082740 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G082740
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Calcium-binding EF hand family protein
Location	chrH04: 22164082 .. 22165695 (+)
RNA-Seq Expression	Chy4G082740
Synteny	Chy4G082740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACCCGGTTAACCGACGTCAAAAGTGTGCAGCTCGACCCATCACCGTTCGTGTTTGTCGATCGGATATCAATTCTACCAATTTTATTCAACTTCGCAGACAGAATACAAGAGCTGGTAAGATCTCTACCTTGGCCAATTCTTCAATCACACTCCAAAGAAAAAGCTTTATCACAATGTAATTGCTCTTCAAAACCTGGTGATCAAATCATCATCAGTACTCAACAAAATTGCAGCTCAATGAGCAGAGAAGAAGTGAAATTTGTGATGGAAAAATTAGAGCTTTTCTGGAGGGAAGAAAGGGATGATGATGGTGGTGATAAAGGAATAGGGGAAAGCGATGAAATGATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCACGATTGCAAAACAATGATAGCCAGATTCGATCTCAATAATGATGGTAAAATTGATTTCCACGAGTTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAGAATATTTTTGGGTTCTACTATTATAATTATTTGTAATAATGTTTTTCATTTTTGTGGACGTGGTGGATATTTTCATTCACATTTTCATATGAAACTAATCCACCTTTTCATTATATATTATAAAGAATTTCCGTAATAGAACAAAAGAATATTGAATAAGATACTAATATGAACAAAGGTAGAAAAATGAAAAGAAAAATGGGGCATAAAATGAAAGATTCAAAGGGCGGGAAAATGAGAAGTTTCGCACACAATTTCTTCCCTATTGGATTGAGAATAGAGTAATTTTAAAATTACAAAATCACATTCAATTTTTTTTTTGTTTTTATTATTCAAATATGAAAGTTTAGATGCAAAGCTTTGACGAATTTAATTGATGCCTTCATGACTAAGCTAAGCTAACTCTTTATTCACATTTGGCCATCACTCATGTTTTTGTTTGCTTTCTTAGTTTTTTTTTTTTTAATTTGTTGAATATTTTGTGTTTTTTTTTATAGAAAAAGGAAAAAGATCCTTCCATCATCAACACATTGTTTTGTATTGGAAATTCCTATGAACATGGTACTGGATTCAACGACAGATTATAAACAAATTATCATTAATCATAACTTTCACACACCTATAGGCTATAGTACAAAATAATATGAAATTCGGTTTTAAAGAAAAGGATATATCTTAATCTAAGTTTGAGATTTTATCAAAATGACGTAAGTGTATAAAAAAACTTCAAGGTTTGAGGTTTGTGATTTTATACCCCACATATATATATTGTGGAGAAACAAAAATTAAACTCAAAATTTTATTAATTTAACATCTGTTAGTTGCAACAACTATCTACTTTTAGTAGAATGAACAAATTTCTGATCCAATATAAACATTTAAATTACCATTTTTCCTTTTCTCAATTCTTCCGGAGGAAATCGATTATTGAGGAAACAAGGCGTGGCGAAATGGATGGTAGGAAAACTTCCATGTCCAATCTCATCTCCTCCAACCCAATCCATGGTTGGGTAG

mRNA sequence

ATGGAAACCCGGTTAACCGACGTCAAAAGTGTGCAGCTCGACCCATCACCGTTCGTGTTTGTCGATCGGATATCAATTCTACCAATTTTATTCAACTTCGCAGACAGAATACAAGAGCTGGTAAGATCTCTACCTTGGCCAATTCTTCAATCACACTCCAAAGAAAAAGCTTTATCACAATGTAATTGCTCTTCAAAACCTGGTGATCAAATCATCATCAGTACTCAACAAAATTGCAGCTCAATGAGCAGAGAAGAAGTGAAATTTGTGATGGAAAAATTAGAGCTTTTCTGGAGGGAAGAAAGGGATGATGATGGTGGTGATAAAGGAATAGGGGAAAGCGATGAAATGATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCACGATTGCAAAACAATGATAGCCAGATTCGATCTCAATAATGATGGAAATCGATTATTGAGGAAACAAGGCGTGGCGAAATGGATGGTAGGAAAACTTCCATGTCCAATCTCATCTCCTCCAACCCAATCCATGGTTGGGTAG

Coding sequence (CDS)

ATGGAAACCCGGTTAACCGACGTCAAAAGTGTGCAGCTCGACCCATCACCGTTCGTGTTTGTCGATCGGATATCAATTCTACCAATTTTATTCAACTTCGCAGACAGAATACAAGAGCTGGTAAGATCTCTACCTTGGCCAATTCTTCAATCACACTCCAAAGAAAAAGCTTTATCACAATGTAATTGCTCTTCAAAACCTGGTGATCAAATCATCATCAGTACTCAACAAAATTGCAGCTCAATGAGCAGAGAAGAAGTGAAATTTGTGATGGAAAAATTAGAGCTTTTCTGGAGGGAAGAAAGGGATGATGATGGTGGTGATAAAGGAATAGGGGAAAGCGATGAAATGATAAGAGGTATGTTTGAAGAGAATGAACCAAGCTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAGAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCACGATTGCAAAACAATGATAGCCAGATTCGATCTCAATAATGATGGAAATCGATTATTGAGGAAACAAGGCGTGGCGAAATGGATGGTAGGAAAACTTCCATGTCCAATCTCATCTCCTCCAACCCAATCCATGGTTGGGTAG

Protein sequence

METRLTDVKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQCNCSSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGNRLLRKQGVAKWMVGKLPCPISSPPTQSMVG*

Homology

[Close]

BLAST of Chy4G082740 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.9e-13
Identity = 43/102 (42.16%), Postives = 62/102 (60.78%), Query Frame = 0


Query: 82  MSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDR 141
           +SREE + VM  L LF+ +++  +        S + +  +FEE E SLEE+KQ F+VFD 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQE------QYSAKEVSSLFEEKEASLEEVKQAFDVFDE 142

Query: 142 NRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 184
           N+DGFID  EL  VL++L   +G ++ +C  MI   D N DG
Sbjct: 143 NKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDG 178

[Close]

BLAST of Chy4G082740 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 53/145 (36.55%), Postives = 79/145 (54.48%), Query Frame = 0


Query: 46  WPILQSHSKEKALSQCNCSSKPGD---QIIISTQQ----NCSSMSREEVKFVMEKLELFW 105
           WP++Q         QC    K  D   Q  I  ++    +   + RE+V  VM+ L L  
Sbjct: 55  WPLVQH-------QQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL-- 114

Query: 106 REERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSL 165
             +++++G  K    S + +  +FEE EPSLEE+KQ F+VFD NRDGFID  +L  VL++
Sbjct: 115 STDQENEGLQKQY--SSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTI 174

Query: 166 LESNKGIFIHDCKTMIARFDLNNDG 184
           L   +G  + +C+ MI  FD + DG
Sbjct: 175 LGLKQGSNLENCRRMIRSFDGSKDG 188

[Close]

BLAST of Chy4G082740 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.6e-08
Identity = 45/120 (37.50%), Postives = 64/120 (53.33%), Query Frame = 0


Query: 66  KPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGD--KGIGESDEMIRGMFE 125
           K GD  I  T +  S   R    ++ +K EL    E+ D  GD    I E  E+ + + +
Sbjct: 15  KNGDGTI--TGKELSETLRSLGIYIPDK-ELTQMIEKIDVNGDGCVDIDEFGELYKTIMD 74

Query: 126 ENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 184
           E +   E++K+ FNVFD+N DGFI   EL  VLS L   +G  + DCK MI + D++ DG
Sbjct: 75  EEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDG 131

[Close]

BLAST of Chy4G082740 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.0e-07
Identity = 30/77 (38.96%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 107 GDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 166
           G   I E   + + M EE E   E++++ F VFD+N DGFI + EL  VL+ +   +G  
Sbjct: 55  GAMDIDEFGSLYQEMVEEKEEE-EDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRT 114

Query: 167 IHDCKTMIARFDLNNDG 184
           + DCK MI++ D++ DG
Sbjct: 115 LEDCKKMISKVDVDGDG 130

[Close]

BLAST of Chy4G082740 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.2e-07
Identity = 37/103 (35.92%), Postives = 57/103 (55.34%), Query Frame = 0


Query: 83  SREEVKFVMEKLELFWREERDDDGGD--KGIGESDEMIRGMFEENEPSLEELKQTFNVFD 142
           S E +   +   +L    E+ D  GD    I E   + + + EE +   E++++ FNVFD
Sbjct: 29  SLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEE-EDMREAFNVFD 88

Query: 143 RNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 184
           +NRDGFI   EL  VL+ L   +G  + DCK MI++ D++ DG
Sbjct: 89  QNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDG 130

[Close]

BLAST of Chy4G082740 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.5e-94
Identity = 176/183 (96.17%), Postives = 179/183 (97.81%), Query Frame = 0


Query: 1   METRLTDVKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQ 60
           METRLTD+KSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQ HSKEKALSQ
Sbjct: 12  METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 71

Query: 61  CNCSSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRG 120
           CNCSSK GDQIII+TQQNCSSMSREEV+FVMEKLELFWREERD  GGDKGIGESDEMIRG
Sbjct: 72  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG 131

Query: 121 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 180
           MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN
Sbjct: 132 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 191

Query: 181 NDG 184
           NDG
Sbjct: 192 NDG 194

[Close]

BLAST of Chy4G082740 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 4.6e-75
Identity = 155/186 (83.33%), Postives = 168/186 (90.32%), Query Frame = 0


Query: 1   METRLTD-VKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WPILQS-HSKEKA 60
           M+T++T+ VKSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W ILQS +SKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 61  LSQCNCSSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEM 120
           LSQ NCSS+  D+III+TQQNC SMSREEV+FVMEKLELFWREE DDD  D+ IG SDE+
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEI 120

Query: 121 IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180
           IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 181 DLNNDG 184
           DLNNDG
Sbjct: 181 DLNNDG 185

[Close]

BLAST of Chy4G082740 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 6.5e-37
Identity = 101/168 (60.12%), Postives = 111/168 (66.07%), Query Frame = 0


Query: 15  PSPFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQCNCSSKPGDQIIIS 74
           P P VF+DR SI      FAD I +LV SL +   QS  KEKA  Q   S   G +  + 
Sbjct: 21  PPPLVFIDRASI------FADTIHDLV-SLAFSFFQSPPKEKAAPQSRASQSEGKEDWL- 80

Query: 75  TQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQ 134
                  MSREEVK VMEKLEL+  EE DD+  D+  G   E +RGMFEENEPSLEELKQ
Sbjct: 81  -------MSREEVKSVMEKLELYCNEEGDDE-IDECFGR--EEVRGMFEENEPSLEELKQ 140

Query: 135 TFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNND 183
           TFNVFDRNRDGFID HEL  VL LL SN  IFIHDCK+MIARFD NND
Sbjct: 141 TFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNND 170

[Close]

BLAST of Chy4G082740 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 1.2e-33
Identity = 100/179 (55.87%), Postives = 113/179 (63.13%), Query Frame = 0


Query: 10  SVQLDPS------PFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQCNC 69
           ++QL PS      P VF+DR SI      FA  I +LV SL +   QS  K+KA  Q   
Sbjct: 9   NLQLIPSTTTTTPPLVFIDRASI------FAGTIHDLV-SLAFSFFQSPPKDKAAPQSRA 68

Query: 70  SSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRGMFE 129
           S         S  +    MSREEVK VMEKLEL+  EE DD+  D+  G  +  +RGMFE
Sbjct: 69  SQ--------SESKEDWLMSREEVKSVMEKLELYCNEEEDDE-IDECFGRDE--VRGMFE 128

Query: 130 ENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNND 183
           ENEPSLEELKQTFNVFDRNRDGFID  EL  VL LL SN  IFIHDCK+MIARFD NND
Sbjct: 129 ENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNND 169

[Close]

BLAST of Chy4G082740 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 3.7e-32
Identity = 90/165 (54.55%), Postives = 106/165 (64.24%), Query Frame = 0


Query: 19  VFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQCNCSSKPGDQIIISTQQN 78
           + VDRISI  ILF+F DRI +L+ S PW +  S  KEKA  Q N           +TQ  
Sbjct: 17  LLVDRISISSILFSFVDRIHDLI-SQPWSLFHSPPKEKASPQGNSLESE------ATQHK 76

Query: 79  CSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQTFNV 138
            SS+S+EEVKFVMEKLE F  +E  ++    G     E I  +F E+EPSLEELKQTFNV
Sbjct: 77  NSSISKEEVKFVMEKLEFFCSDEEIEESWLGG-----EEIGAIFYESEPSLEELKQTFNV 136

Query: 139 FDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 184
           FD+NRDG ID  EL  VL LL  N+G+FIHDC  MIARFD N DG
Sbjct: 137 FDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDG 169

[Close]

BLAST of Chy4G082740 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 352 bits (904), Expect = 1.83e-121
Identity = 176/183 (96.17%), Postives = 179/183 (97.81%), Query Frame = 0


Query: 1   METRLTDVKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQ 60
           METRLTD+KSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQ HSKEKALSQ
Sbjct: 1   METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 60

Query: 61  CNCSSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRG 120
           CNCSSK GDQIII+TQQNCSSMSREEV+FVMEKLELFWREERD  GGDKGIGESDEMIRG
Sbjct: 61  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG 120

Query: 121 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 180
           MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN
Sbjct: 121 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 180

Query: 181 NDG 183
           NDG
Sbjct: 181 NDG 183

[Close]

BLAST of Chy4G082740 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 352 bits (904), Expect = 2.70e-121
Identity = 176/183 (96.17%), Postives = 179/183 (97.81%), Query Frame = 0


Query: 1   METRLTDVKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQ 60
           METRLTD+KSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQ HSKEKALSQ
Sbjct: 12  METRLTDIKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQ 71

Query: 61  CNCSSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRG 120
           CNCSSK GDQIII+TQQNCSSMSREEV+FVMEKLELFWREERD  GGDKGIGESDEMIRG
Sbjct: 72  CNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRG 131

Query: 121 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 180
           MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN
Sbjct: 132 MFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLN 191

Query: 181 NDG 183
           NDG
Sbjct: 192 NDG 194

[Close]

BLAST of Chy4G082740 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 289 bits (740), Expect = 1.86e-96
Identity = 155/186 (83.33%), Postives = 168/186 (90.32%), Query Frame = 0


Query: 1   METRLTD-VKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WPILQS-HSKEKA 60
           M+T++T+ VKSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W ILQS +SKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 61  LSQCNCSSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEM 120
           LSQ NCSS+  D+III+TQQNCS MSREEV+FVMEKLELFWREE DDD  D+ IG SDE+
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCS-MSREEVRFVMEKLELFWREENDDDDDDEEIGASDEI 120

Query: 121 IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180
           IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 181 DLNNDG 183
           DLNNDG
Sbjct: 181 DLNNDG 185

[Close]

BLAST of Chy4G082740 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 211 bits (538), Expect = 7.19e-66
Identity = 123/185 (66.49%), Postives = 138/185 (74.59%), Query Frame = 0


Query: 1   METRLTDVKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQ 60
           M+ RL DV +VQL P+P VFVDR SILPILF FADRI +L+ S+P    QS SKEKA  Q
Sbjct: 1   MQNRLPDV-NVQLIPTPLVFVDRTSILPILFTFADRIHDLI-SVPLSFFQSPSKEKASPQ 60

Query: 61  CNCSSKPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGE--SDEMI 120
            NCSS+  DQ+I  TQQ    MSREEV+FVM+KLELF  +    + GD+ IGE    E +
Sbjct: 61  SNCSSQSDDQVI--TQQKNCLMSREEVRFVMDKLELFCSK----NDGDEEIGECFGGEEL 120

Query: 121 RGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 180
             MFEENEPSLEELKQTFNVFDRNRDGFID  EL IVL LL+SN GIFIHDCK MIARFD
Sbjct: 121 TAMFEENEPSLEELKQTFNVFDRNRDGFIDAEELHIVLGLLQSNNGIFIHDCKRMIARFD 177

Query: 181 LNNDG 183
            NNDG
Sbjct: 181 HNNDG 177

[Close]

BLAST of Chy4G082740 vs. NCBI nr
Match: XP_022983385.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 162 bits (410), Expect = 1.44e-46
Identity = 101/168 (60.12%), Postives = 111/168 (66.07%), Query Frame = 0


Query: 15  PSPFVFVDRISILPILFNFADRIQELVRSLPWPILQSHSKEKALSQCNCSSKPGDQIIIS 74
           P P VF+DR SI      FAD I +LV SL +   QS  KEKA  Q   S   G +  + 
Sbjct: 21  PPPLVFIDRASI------FADTIHDLV-SLAFSFFQSPPKEKAAPQSRASQSEGKEDWL- 80

Query: 75  TQQNCSSMSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQ 134
                  MSREEVK VMEKLEL+  EE DD+  D+  G   E +RGMFEENEPSLEELKQ
Sbjct: 81  -------MSREEVKSVMEKLELYCNEEGDDEI-DECFGR--EEVRGMFEENEPSLEELKQ 140

Query: 135 TFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNND 182
           TFNVFDRNRDGFID HEL  VL LL SN  IFIHDCK+MIARFD NND
Sbjct: 141 TFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNND 170

[Close]

BLAST of Chy4G082740 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.5e-14
Identity = 43/102 (42.16%), Postives = 62/102 (60.78%), Query Frame = 0


Query: 82  MSREEVKFVMEKLELFWREERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDR 141
           +SREE + VM  L LF+ +++  +        S + +  +FEE E SLEE+KQ F+VFD 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQE------QYSAKEVSSLFEEKEASLEEVKQAFDVFDE 142

Query: 142 NRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 184
           N+DGFID  EL  VL++L   +G ++ +C  MI   D N DG
Sbjct: 143 NKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDG 178

[Close]

BLAST of Chy4G082740 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 75.1 bits (183), Expect = 7.7e-14
Identity = 53/145 (36.55%), Postives = 79/145 (54.48%), Query Frame = 0


Query: 46  WPILQSHSKEKALSQCNCSSKPGD---QIIISTQQ----NCSSMSREEVKFVMEKLELFW 105
           WP++Q         QC    K  D   Q  I  ++    +   + RE+V  VM+ L L  
Sbjct: 55  WPLVQH-------QQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL-- 114

Query: 106 REERDDDGGDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSL 165
             +++++G  K    S + +  +FEE EPSLEE+KQ F+VFD NRDGFID  +L  VL++
Sbjct: 115 STDQENEGLQKQY--SSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTI 174

Query: 166 LESNKGIFIHDCKTMIARFDLNNDG 184
           L   +G  + +C+ MI  FD + DG
Sbjct: 175 LGLKQGSNLENCRRMIRSFDGSKDG 188

[Close]

BLAST of Chy4G082740 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-09
Identity = 45/120 (37.50%), Postives = 64/120 (53.33%), Query Frame = 0


Query: 66  KPGDQIIISTQQNCSSMSREEVKFVMEKLELFWREERDDDGGD--KGIGESDEMIRGMFE 125
           K GD  I  T +  S   R    ++ +K EL    E+ D  GD    I E  E+ + + +
Sbjct: 15  KNGDGTI--TGKELSETLRSLGIYIPDK-ELTQMIEKIDVNGDGCVDIDEFGELYKTIMD 74

Query: 126 ENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 184
           E +   E++K+ FNVFD+N DGFI   EL  VLS L   +G  + DCK MI + D++ DG
Sbjct: 75  EEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDG 131

[Close]

BLAST of Chy4G082740 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 56.6 bits (135), Expect = 2.8e-08
Identity = 30/77 (38.96%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 107 GDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 166
           G   I E   + + M EE E   E++++ F VFD+N DGFI + EL  VL+ +   +G  
Sbjct: 55  GAMDIDEFGSLYQEMVEEKEEE-EDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRT 114

Query: 167 IHDCKTMIARFDLNNDG 184
           + DCK MI++ D++ DG
Sbjct: 115 LEDCKKMISKVDVDGDG 130

[Close]

BLAST of Chy4G082740 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 56.2 bits (134), Expect = 3.7e-08
Identity = 37/103 (35.92%), Postives = 57/103 (55.34%), Query Frame = 0


Query: 83  SREEVKFVMEKLELFWREERDDDGGD--KGIGESDEMIRGMFEENEPSLEELKQTFNVFD 142
           S E +   +   +L    E+ D  GD    I E   + + + EE +   E++++ FNVFD
Sbjct: 29  SLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEE-EDMREAFNVFD 88

Query: 143 RNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 184
           +NRDGFI   EL  VL+ L   +G  + DCK MI++ D++ DG
Sbjct: 89  QNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDG 130

The following BLAST results are available for this feature:

BLAST of Chy4G082740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	4.9e-13	42.16	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	1.1e-12	36.55	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9LNE7	1.6e-08	37.50	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SU00	4.0e-07	38.96	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9SRR7	5.2e-07	35.92	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]

BLAST of Chy4G082740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ5	4.5e-94	96.17	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]
A0A1S3B8I1	4.6e-75	83.33	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]
A0A6J1J5Q6	6.5e-37	60.12	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1F854	1.2e-33	55.87	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1EIK8	3.7e-32	54.55	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of Chy4G082740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147503.1	1.83e-121	96.17	probable calcium-binding protein CML45 [Cucumis sativus]	[more]
KGN59556.1	2.70e-121	96.17	hypothetical protein Csa_001548 [Cucumis sativus]	[more]
XP_008443616.1	1.86e-96	83.33	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
XP_038904514.1	7.19e-66	66.49	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
XP_022983385.1	1.44e-46	60.12	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]

BLAST of Chy4G082740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	3.5e-14	42.16	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	7.7e-14	36.55	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	1.1e-09	37.50	EF hand calcium-binding protein family	[more]
AT4G12860.1	2.8e-08	38.96	EF hand calcium-binding protein family	[more]
AT3G07490.1	3.7e-08	35.92	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 131..159 e-value: 2.4E-5 score: 33.8
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 131..157 e-value: 4.2E-6 score: 25.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..162 score: 13.537066
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 131..183 e-value: 3.03108E-9 score: 49.4685
None	No IPR available	GENE3D	1.10.238.10		coord: 68..190 e-value: 6.4E-12 score: 47.7
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 5..183
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..183
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 81..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G082740.1	Chy4G082740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding