Chy4G082650 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G082650
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Ribonuclease MC-like
Location	chrH04: 22108591 .. 22109448 (+)
RNA-Seq Expression	Chy4G082650
Synteny	Chy4G082650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGTAGCAATGGCAAGGGGAAGGGGATCAGAAATTGTGTTATTGTTCGTGGTTACCTTAAGCATCGGCTTGTTTCCTATTATGAAATCACAACAATTTGATAACTTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCAATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTTCTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATCAATTGCCCTGGCACCCAATTTGATTTCAATAAGGTATATTCTATTTTAGAATTCTCAAATCCACTCTATAAATTTTCCTCTAAATTTTCCTCTGTCTATCTATATCTATATCTATATATATTATTTAAATAACTTTGAATAAAAAGAACGATTTGAAAATAAATACGTAGTTTAGTCTCAACACGACTATTTTTAACATTACGTGCGAGTGAGTTTCAGATTTCGACTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAATGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAAATCAAGATCAACGTTCTTAGTGCCCTGAGGATGGGTGGGATAACGCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCAATGTTCACTGCATATCACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACGATGATGGCGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGA

mRNA sequence

ATGGTGGTAGCAATGGCAAGGGGAAGGGGATCAGAAATTGTGTTATTGTTCGTGGTTACCTTAAGCATCGGCTTGTTTCCTATTATGAAATCACAACAATTTGATAACTTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCAATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTTCTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATCAATTGCCCTGGCACCCAATTTGATTTCAATAAGATTTCGACTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAATGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAAATCAAGATCAACGTTCTTAGTGCCCTGAGGATGGGTGGGATAACGCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCAATGTTCACTGCATATCACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACGATGATGGCGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGA

Coding sequence (CDS)

ATGGTGGTAGCAATGGCAAGGGGAAGGGGATCAGAAATTGTGTTATTGTTCGTGGTTACCTTAAGCATCGGCTTGTTTCCTATTATGAAATCACAACAATTTGATAACTTTTACTTTGTTCAACAATGGCCACCAGCTGTTTGCAATGGACGTACAGGAAAATGTGTAGGAAAAGGGATGTACTTCTTTACAATTCATGGTGTGTGGCCTCAGAAAGGAGGAAAATCTGTAATCAATTGCCCTGGCACCCAATTTGATTTCAATAAGATTTCGACTTTAGCAAACACTCTACATCAAATTATGAAGGATGTGATAAACGCAGACGACCAATTTTTATGGTCTCACGAATGGAACAAACATGGAGTTTGCTCAGAAAGCAGGTACTCAATGAAACAATACTTCCAAATGGCGATAAATATGAAAATCAAGATCAACGTTCTTAGTGCCCTGAGGATGGGTGGGATAACGCCAAACAACCACTTGAAGGCCAAACAAAGAGTAGAGGGGGCAATGTTCACTGCATATCACGCATATCCTCTTCTTCGATGTAAAAAAGATTCATCTGGCCAATCCCTATTGACGGAGGTTGTTATGTGCTTTGACGATGATGGCGTCACCCTTCTAAACTGCACTACAACGAAATCCAATTGTGATGCAGACGTTCTCTTTTGA

Protein sequence

MVVAMARGRGSEIVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQKGGKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLTEVVMCFDDDGVTLLNCTTTKSNCDADVLF*

Homology

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BLAST of Chy4G082650 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 5.9e-46
Identity = 85/193 (44.04%), Postives = 126/193 (65.28%), Query Frame = 0


Query: 34  FDNFYFVQQWPPAVCN-GRTGKCVGKGMYFFTIHGVWPQKGGKSVINCP-GTQFDFNKIS 93
           FD+F+FVQQWPPAVC+  ++G C G G+  FTIHG+WPQ  G S+ NCP G+ FD  KIS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 94  TLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKIKINVLSALR 153
            L + L+ +  +V+ A++Q  WSHEW KHG CSES ++   YF++A++M+   +++ ALR
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 154 MGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQ-SLLTEVVMCFDDDGVTLLNC 213
                PN   K++Q ++G +   +  +P LRC+ D   + S L +VV CF  DG TL++C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 214 TTTKSNCDADVLF 224
             T+  C A+ +F
Sbjct: 181 --TRDTCGANFIF 191

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BLAST of Chy4G082650 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.3e-29
Identity = 71/201 (35.32%), Postives = 103/201 (51.24%), Query Frame = 0


Query: 31  SQQFDNFYFVQQWPPAVCNGRTGKCV---GKGMYFFTIHGVWPQ-KGGKSVINCPGTQ-F 90
           S+ FD FYFVQQWP + C+ +   C    GK    F IHG+WP  K G    NC  ++ F
Sbjct: 27  SEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPF 86

Query: 91  DFNKISTLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKIK 150
           D + IS L  ++ +    +   +   +  W HEW KHG CSES     +YFQ A+N+K K
Sbjct: 87  DSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQK 146

Query: 151 INVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLTEVVMCFDDD 210
            N+L AL   GI P+    + + +  ++  +    P + C +D SG S L +V +C D  
Sbjct: 147 TNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

Query: 211 GVTLLNCTT-TKSNCDADVLF 224
           G  L+ C       C A++ F
Sbjct: 207 GSGLIECPVFPHGKCGAEIEF 227

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BLAST of Chy4G082650 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.5e-28
Identity = 71/201 (35.32%), Postives = 99/201 (49.25%), Query Frame = 0


Query: 31  SQQFDNFYFVQQWPPAVCNGRTGKC---VGKGMYFFTIHGVWPQ-KGGKSVINC-PGTQF 90
           +Q FD FYFV QWP A C+ R   C    GK    F IHG+WP  K G    NC P ++F
Sbjct: 19  AQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRF 78

Query: 91  DFNKISTLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKIK 150
           D  ++S L + L +    +   + D    W+HEW KHG C+ES      YF+  + +K K
Sbjct: 79  DDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQK 138

Query: 151 INVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLTEVVMCFDDD 210
            N+L AL   GI P++     + +E  +       P + C KDSS  S L ++ +C D  
Sbjct: 139 ANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTS 198

Query: 211 GVTLLNC-TTTKSNCDADVLF 224
               +NC       CD+ V F
Sbjct: 199 ASKFINCPVMPHGRCDSRVQF 219

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BLAST of Chy4G082650 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 5.2e-26
Identity = 68/208 (32.69%), Postives = 111/208 (53.37%), Query Frame = 0


Query: 11  SEIVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKC---VGKGMYFFTIHG 70
           S  ++L ++T  + +  +  ++ FD FYFVQQWP + C+ +   C    GK    F IHG
Sbjct: 8   SLFLILLIITQCLSV--LNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHG 67

Query: 71  VWPQKG-GKSVINC-PGTQFDFNKISTLANTLHQIMKDVI--NADDQFLWSHEWNKHGVC 130
           +WP    G    NC P + +D ++IS L +++ Q    +   +      WSHEW KHG C
Sbjct: 68  LWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTC 127

Query: 131 SESRYSMKQ-YFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLR 190
           +ES  + +  YF+ A+++K +I++LS L+   I P+        +  A+ +A    P ++
Sbjct: 128 AESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQ 187

Query: 191 CKKDSSGQSLLTEVVMCFDDDGVTLLNC 211
           C  D SG S L +V +C D  G +L+ C
Sbjct: 188 CNVDQSGNSQLYQVYICVDGSGSSLIEC 213

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BLAST of Chy4G082650 vs. ExPASy Swiss-Prot
Match: Q38716 (Ribonuclease S-2 OS=Antirrhinum hispanicum OX=49039 GN=S2 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.6e-21
Identity = 63/216 (29.17%), Postives = 95/216 (43.98%), Query Frame = 0


Query: 2   VVAMARGRGSEIVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGM- 61
           +  + + + S   LL    + +  F    + QFD F  V QWP + C+ +T  C    + 
Sbjct: 1   MATVQKSQHSHFFLLVGCIVHLSNFCSTTTAQFDYFKLVLQWPNSYCSLKTTHCPRTRLP 60

Query: 62  YFFTIHGVWPQKGGKSVINCPGTQFDFNKIS------TLANTLHQIMKDVINADDQFLWS 121
             FTIHG+WP      + NC  T  D  KI+       LA     + +       Q  W 
Sbjct: 61  SQFTIHGLWPDNKSWPLSNCRDTSADVLKITDKGLIQDLAVHWPDLTRRQRKVPGQKFWV 120

Query: 122 HEWNKHGVCSESRYSMKQYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTA 181
            +W KHG C+   YS   YF  A+ +K + NVL  L    +TP +       V GA+   
Sbjct: 121 TQWKKHGACALPMYSFNDYFVKALELKKRNNVLDMLSRKSLTPGDQRVDVSDVNGAITKV 180

Query: 182 YHAYPLLRCKKDSSGQSLLTEVVMCFDDDGVTLLNC 211
                +L+C      +  LTEV++CFD  G  +++C
Sbjct: 181 TGGIAILKCP-----EGYLTEVIICFDPSGFPVIDC 211

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BLAST of Chy4G082650 vs. ExPASy TrEMBL
Match: A0A0A0LHQ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G825060 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.6e-118
Identity = 208/221 (94.12%), Postives = 214/221 (96.83%), Query Frame = 0


Query: 5   MARGRGSE--IVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYF 64
           MA GR S+  IVLLFVVTLSI LFPIMKSQQFD FYFVQQWPPAVC+GRTGKCVGKGMY+
Sbjct: 1   MAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYY 60

Query: 65  FTIHGVWPQKGGKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGV 124
           FTIHGVWPQKGGKSVINCPGTQFDFNKIS+LANTLHQIMKDVINADDQFLWSHEWNKHGV
Sbjct: 61  FTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGV 120

Query: 125 CSESRYSMKQYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLR 184
           CSESRYSMKQYFQMAINMK KINVLSALRMGGITPNNHLKAKQRVEGAMFTAY+AYPLLR
Sbjct: 121 CSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLR 180

Query: 185 CKKDSSGQSLLTEVVMCFDDDGVTLLNCTTTKSNCDADVLF 224
           CKKDSSGQSLLTEVVMCFD+DGVTLLNCTTTKSNCDADVLF
Sbjct: 181 CKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 221

[Close]

BLAST of Chy4G082650 vs. ExPASy TrEMBL
Match: A0A5A7UJ53 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007970 PE=3 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.7e-91
Identity = 157/210 (74.76%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 14  VLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQKG 73
           +LLFVVTLSI LFP MKSQ FD+FYFVQQWPPAVC GR+G+CVG+G  +FTIHGVWPQKG
Sbjct: 1   MLLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKG 60

Query: 74  GKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 133
           G+S++NCPGT+FD N+IS+L NTLHQ   DVIN  DQ  W HEWNKHG CSES+Y M  Y
Sbjct: 61  GQSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKYQMLPY 120

Query: 134 FQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLL 193
           FQMAINMK KI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LL
Sbjct: 121 FQMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLL 180

Query: 194 TEVVMCFDDDGVTLLNCTTTKSNCDADVLF 224
           TEVVMCF DDGVTLLNCT +KS C AD LF
Sbjct: 181 TEVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

[Close]

BLAST of Chy4G082650 vs. ExPASy TrEMBL
Match: A0A5D3DQ69 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007950 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.8e-91
Identity = 156/210 (74.29%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 14  VLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQKG 73
           +LLFVVTLSI LFP MKSQ FD+FYFVQQWPPAVC GR+G+CVG+G  +FTIHGVWPQKG
Sbjct: 1   MLLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKG 60

Query: 74  GKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 133
           G+S++NCPGT+FD N+IS+L NTLHQ   DVIN  DQ  W HEWNKHG CSES++ M  Y
Sbjct: 61  GQSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKFQMLPY 120

Query: 134 FQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLL 193
           FQMAINMK KI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LL
Sbjct: 121 FQMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLL 180

Query: 194 TEVVMCFDDDGVTLLNCTTTKSNCDADVLF 224
           TEVVMCF DDGVTLLNCT +KS C AD LF
Sbjct: 181 TEVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

[Close]

BLAST of Chy4G082650 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 3.0e-69
Identity = 123/212 (58.02%), Postives = 164/212 (77.36%), Query Frame = 0


Query: 12  EIVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQ 71
           EIV +FV+ ++I L PI+KSQ FD+F+FVQQWPPAVC+ + G+CVG+G+  FTIHGVWPQ
Sbjct: 7   EIVFMFVLAITIFL-PIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIHGVWPQ 66

Query: 72  KGGKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMK 131
           KGG SV+NCPGT FDF KIS+L   L+++  +VI  D++F WSHEWNKHGVCSES+++MK
Sbjct: 67  KGGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSESKFNMK 126

Query: 132 QYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQS 191
            YFQMAINM+  I++++AL++GG+ PN   K+KQRV+ A+ T +   P+LRC+   S   
Sbjct: 127 AYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIR 186

Query: 192 LLTEVVMCFDDDGVTLLNCTTTKSNCDADVLF 224
           L+ E+VMCFDDDGVTL+NC    SNC    +F
Sbjct: 187 LI-EIVMCFDDDGVTLINCNPVSSNCANSFIF 216

[Close]

BLAST of Chy4G082650 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 4.0e-69
Identity = 123/210 (58.57%), Postives = 159/210 (75.71%), Query Frame = 0


Query: 14  VLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQKG 73
           V+ FV  L+I LFPI+KSQ FD+F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKG
Sbjct: 9   VVFFVFVLTI-LFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKG 68

Query: 74  GKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 133
           G+SV NC G QFDF KI+ L N L+ +  +V+  +++F W HEWNKHG+CSES++   +Y
Sbjct: 69  GRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKY 128

Query: 134 FQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLL 193
           FQ AINM+  I++LS LR GG+ PN   KAKQRVE A+ + +   P+LRCKK S+GQ LL
Sbjct: 129 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 188

Query: 194 TEVVMCFDDDGVTLLNCTTTKSNCDADVLF 224
           TE+VMCFDDDGVTL+NC   +SNC    +F
Sbjct: 189 TEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of Chy4G082650 vs. NCBI nr
Match: XP_004147497.3 (ribonuclease MC [Cucumis sativus] >KAE8651218.1 hypothetical protein Csa_002248 [Cucumis sativus])

HSP 1 Score: 436 bits (1122), Expect = 3.93e-154
Identity = 212/225 (94.22%), Postives = 218/225 (96.89%), Query Frame = 0


Query: 1   MVVAMARGRGSEI--VLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGK 60
           MVVAMA GR S+I  VLLFVVTLSI LFPIMKSQQFD FYFVQQWPPAVC+GRTGKCVGK
Sbjct: 1   MVVAMAGGRRSDINIVLLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGK 60

Query: 61  GMYFFTIHGVWPQKGGKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWN 120
           GMY+FTIHGVWPQKGGKSVINCPGTQFDFNKIS+LANTLHQIMKDVINADDQFLWSHEWN
Sbjct: 61  GMYYFTIHGVWPQKGGKSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWN 120

Query: 121 KHGVCSESRYSMKQYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAY 180
           KHGVCSESRYSMKQYFQMAINMK KINVLSALRMGGITPNNHLKAKQRVEGAMFTAY+AY
Sbjct: 121 KHGVCSESRYSMKQYFQMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAY 180

Query: 181 PLLRCKKDSSGQSLLTEVVMCFDDDGVTLLNCTTTKSNCDADVLF 223
           PLLRCKKDSSGQSLLTEVVMCFD+DGVTLLNCTTTKSNCDADVLF
Sbjct: 181 PLLRCKKDSSGQSLLTEVVMCFDNDGVTLLNCTTTKSNCDADVLF 225

[Close]

BLAST of Chy4G082650 vs. NCBI nr
Match: KAA0053765.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25637.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 341 bits (875), Expect = 1.01e-116
Identity = 157/209 (75.12%), Postives = 176/209 (84.21%), Query Frame = 0


Query: 15  LLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQKGG 74
           LLFVVTLSI LFP MKSQ FD+FYFVQQWPPAVC GR+G+CVG+G  +FTIHGVWPQKGG
Sbjct: 2   LLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKGG 61

Query: 75  KSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 134
           +S++NCPGT+FD N+IS+L NTLHQ   DVIN  DQ  W HEWNKHG CSES+Y M  YF
Sbjct: 62  QSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKYQMLPYF 121

Query: 135 QMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLT 194
           QMAINMK KI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LLT
Sbjct: 122 QMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLLT 181

Query: 195 EVVMCFDDDGVTLLNCTTTKSNCDADVLF 223
           EVVMCF DDGVTLLNCT +KS C AD LF
Sbjct: 182 EVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

[Close]

BLAST of Chy4G082650 vs. NCBI nr
Match: KAA0053767.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25635.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 340 bits (871), Expect = 4.10e-116
Identity = 156/209 (74.64%), Postives = 176/209 (84.21%), Query Frame = 0


Query: 15  LLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQKGG 74
           LLFVVTLSI LFP MKSQ FD+FYFVQQWPPAVC GR+G+CVG+G  +FTIHGVWPQKGG
Sbjct: 2   LLFVVTLSISLFPTMKSQIFDDFYFVQQWPPAVCAGRSGQCVGQGFRYFTIHGVWPQKGG 61

Query: 75  KSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 134
           +S++NCPGT+FD N+IS+L NTLHQ   DVIN  DQ  W HEWNKHG CSES++ M  YF
Sbjct: 62  QSIVNCPGTKFDVNQISSLTNTLHQTWPDVINGKDQTFWEHEWNKHGTCSESKFQMLPYF 121

Query: 135 QMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLT 194
           QMAINMK KI+VL A+R+GG+ PNNH KAKQRVE  +F AYHAYPLLRCKKDS+GQ LLT
Sbjct: 122 QMAINMKYKIDVLGAMRVGGVVPNNHKKAKQRVEVVIFNAYHAYPLLRCKKDSTGQYLLT 181

Query: 195 EVVMCFDDDGVTLLNCTTTKSNCDADVLF 223
           EVVMCF DDGVTLLNCT +KS C AD LF
Sbjct: 182 EVVMCFKDDGVTLLNCTRSKSTCGADFLF 210

[Close]

BLAST of Chy4G082650 vs. NCBI nr
Match: XP_022155091.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 267 bits (683), Expect = 2.05e-87
Identity = 123/212 (58.02%), Postives = 164/212 (77.36%), Query Frame = 0


Query: 12  EIVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQ 71
           EIV +FV+ ++I L PI+KSQ FD+F+FVQQWPPAVC+ + G+CVG+G+  FTIHGVWPQ
Sbjct: 7   EIVFMFVLAITIFL-PIVKSQTFDDFWFVQQWPPAVCSFQQGRCVGQGLRTFTIHGVWPQ 66

Query: 72  KGGKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMK 131
           KGG SV+NCPGT FDF KIS+L   L+++  +VI  D++F WSHEWNKHGVCSES+++MK
Sbjct: 67  KGGTSVVNCPGTPFDFTKISSLETDLNRVWPNVITGDNKFFWSHEWNKHGVCSESKFNMK 126

Query: 132 QYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQS 191
            YFQMAINM+  I++++AL++GG+ PN   K+KQRV+ A+ T +   P+LRC+   S   
Sbjct: 127 AYFQMAINMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIR 186

Query: 192 LLTEVVMCFDDDGVTLLNCTTTKSNCDADVLF 223
           L+ E+VMCFDDDGVTL+NC    SNC    +F
Sbjct: 187 LI-EIVMCFDDDGVTLINCNPVSSNCANSFIF 216

[Close]

BLAST of Chy4G082650 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 267 bits (682), Expect = 3.01e-87
Identity = 123/210 (58.57%), Postives = 159/210 (75.71%), Query Frame = 0


Query: 14  VLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCVGKGMYFFTIHGVWPQKG 73
           V+ FV  L+I LFPI+KSQ FD+F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKG
Sbjct: 9   VVFFVFVLTI-LFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKG 68

Query: 74  GKSVINCPGTQFDFNKISTLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQY 133
           G+SV NC G QFDF KI+ L N L+ +  +V+  +++F W HEWNKHG+CSES++   +Y
Sbjct: 69  GRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKY 128

Query: 134 FQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLL 193
           FQ AINM+  I++LS LR GG+ PN   KAKQRVE A+ + +   P+LRCKK S+GQ LL
Sbjct: 129 FQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLL 188

Query: 194 TEVVMCFDDDGVTLLNCTTTKSNCDADVLF 223
           TE+VMCFDDDGVTL+NC   +SNC    +F
Sbjct: 189 TEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of Chy4G082650 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 131.3 bits (329), Expect = 9.4e-31
Identity = 71/201 (35.32%), Postives = 103/201 (51.24%), Query Frame = 0


Query: 31  SQQFDNFYFVQQWPPAVCNGRTGKCV---GKGMYFFTIHGVWPQ-KGGKSVINCPGTQ-F 90
           S+ FD FYFVQQWP + C+ +   C    GK    F IHG+WP  K G    NC  ++ F
Sbjct: 27  SEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPF 86

Query: 91  DFNKISTLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKIK 150
           D + IS L  ++ +    +   +   +  W HEW KHG CSES     +YFQ A+N+K K
Sbjct: 87  DSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQK 146

Query: 151 INVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLTEVVMCFDDD 210
            N+L AL   GI P+    + + +  ++  +    P + C +D SG S L +V +C D  
Sbjct: 147 TNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

Query: 211 GVTLLNCTT-TKSNCDADVLF 224
           G  L+ C       C A++ F
Sbjct: 207 GSGLIECPVFPHGKCGAEIEF 227

[Close]

BLAST of Chy4G082650 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-29
Identity = 71/201 (35.32%), Postives = 99/201 (49.25%), Query Frame = 0


Query: 31  SQQFDNFYFVQQWPPAVCNGRTGKC---VGKGMYFFTIHGVWPQ-KGGKSVINC-PGTQF 90
           +Q FD FYFV QWP A C+ R   C    GK    F IHG+WP  K G    NC P ++F
Sbjct: 19  AQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRF 78

Query: 91  DFNKISTLANTLHQIMKDVI--NADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKIK 150
           D  ++S L + L +    +   + D    W+HEW KHG C+ES      YF+  + +K K
Sbjct: 79  DDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQK 138

Query: 151 INVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRCKKDSSGQSLLTEVVMCFDDD 210
            N+L AL   GI P++     + +E  +       P + C KDSS  S L ++ +C D  
Sbjct: 139 ANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVDTS 198

Query: 211 GVTLLNC-TTTKSNCDADVLF 224
               +NC       CD+ V F
Sbjct: 199 ASKFINCPVMPHGRCDSRVQF 219

[Close]

BLAST of Chy4G082650 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 125.9 bits (315), Expect = 4.0e-29
Identity = 73/223 (32.74%), Postives = 109/223 (48.88%), Query Frame = 0


Query: 9   RGSEIVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKCV---GKGMYFFTI 68
           +G   VLL ++    GLF     + FD FYFV QWP A C+     C    GK    F I
Sbjct: 4   KGCVNVLLKLLVFQ-GLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGI 63

Query: 69  HGVWPQ-KGGKSVINC-PGTQFDFNKISTLANTLHQIMKDVINADDQ--FLWSHEWNKHG 128
           HG+WP   GG    NC P +QFD ++IS L ++L +    +    ++    W HEW KHG
Sbjct: 64  HGLWPNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHG 123

Query: 129 VCSESRYSMKQYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLL 188
            CSES     +YF+ A+ +K K N+L  L+  GI P++      ++  A+       P +
Sbjct: 124 TCSESVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGI 183

Query: 189 RCKKDSSGQSLLTEVVMCFDDDGVTLLNC-TTTKSNCDADVLF 224
            C KD    + L ++ +C D  G   + C    + +C + + F
Sbjct: 184 ECNKDPERNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQF 225

[Close]

BLAST of Chy4G082650 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 108.2 bits (269), Expect = 8.5e-24
Identity = 60/207 (28.99%), Postives = 99/207 (47.83%), Query Frame = 0


Query: 13  IVLLFVVTLSIGLFPIMKSQQFDNFYFVQQWPPAVCNGRTGKC---VGKGMYFFTIHGVW 72
           I++  ++  S+ +        F+ FY+V  WP A+C+ + G C    G     F IHG+W
Sbjct: 5   IIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLW 64

Query: 73  PQ-KGGKSVINCPGTQ-FDFNKISTLANTLHQIMKD----VINADDQFLWSHEWNKHGVC 132
           PQ   G     C  T  FD +KIS L   + +   +       +++  LW HEWNKHG C
Sbjct: 65  PQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTC 124

Query: 133 SESRYSMKQYFQMAINMKIKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYHAYPLLRC 192
            +S +    YF+  +  K K+++L+ L   GI PN+   +   ++ A+  A    P + C
Sbjct: 125 VQSIFDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIEC 184

Query: 193 KKDSSGQSLLTEVVMCFDDDGVTLLNC 211
            +D  G   L ++ +C D+     + C
Sbjct: 185 NEDVKGNKQLFQIYICLDNYAKEFVEC 211

The following BLAST results are available for this feature:

BLAST of Chy4G082650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	5.9e-46	44.04	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	1.3e-29	35.32	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	2.5e-28	35.32	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P80022	5.2e-26	32.69	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
Q38716	8.6e-21	29.17	Ribonuclease S-2 OS=Antirrhinum hispanicum OX=49039 GN=S2 PE=2 SV=1	[more]

BLAST of Chy4G082650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHQ9	1.6e-118	94.12	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G825060 PE=3 SV=1	[more]
A0A5A7UJ53	1.7e-91	74.76	Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]
A0A5D3DQ69	4.8e-91	74.29	Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]
A0A6J1DQN7	3.0e-69	58.02	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1	[more]
A0A0A0LC37	4.0e-69	58.57	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]

BLAST of Chy4G082650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147497.3	3.93e-154	94.22	ribonuclease MC [Cucumis sativus] >KAE8651218.1 hypothetical protein Csa_002248 ...	[more]
KAA0053765.1	1.01e-116	75.12	ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25637.1 ribonuclease MC-like...	[more]
KAA0053767.1	4.10e-116	74.64	ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25635.1 ribonuclease MC-like...	[more]
XP_022155091.1	2.05e-87	58.02	ribonuclease MC-like [Momordica charantia]	[more]
XP_004147499.1	3.01e-87	58.57	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]

BLAST of Chy4G082650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	9.4e-31	35.32	ribonuclease 1	[more]
AT1G26820.1	1.8e-29	35.32	ribonuclease 3	[more]
AT1G14220.1	4.0e-29	32.74	Ribonuclease T2 family protein	[more]
AT1G14210.1	8.5e-24	28.99	Ribonuclease T2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 34..222 e-value: 2.9E-49 score: 169.6
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 34..223
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 34..210 e-value: 5.0E-38 score: 131.1
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 19..218
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 19..218
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 112..123
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 34..223 e-value: 9.97437E-63 score: 192.162

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G082650.1	Chy4G082650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding