Chy4G082120 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G082120
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	mitogen-activated protein kinase kinase kinase YODA-like
Location	chrH04: 21595957 .. 21596949 (-)
RNA-Seq Expression	Chy4G082120
Synteny	Chy4G082120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAAAATCCTGTGTTGTTTCACAAGTGCAAATCTCTCGCAACCTGCACGAATTCTTGAAAACAAAGAGAAGAATTCTGAAGATTGCAAAGAAATCGGCACCAACAAACAATTGGAATGACACCATCAAACATAATTATGAAAAATTTGGTGAAGATTTCACAATCGAAGAATTCCATGGCTCAATTTGGACTGGTTTTCTTCTCTTTCGTAAAACAGAATCTACCCTAATTTTCTTGGCGAAGAAGAACAGAGCACCCATTAACAACTCAAATTCAAATCTCCCTGAAGAATTTATTGTCAAATCTTCTTTAATGGAGTCCTCTTCTTCGTTACGTCATGAGAAGGAAGTGCTATCCAATCTCGGACCATTCACTAAACTAGTGGATTGTTATGGAGATGAAATTACAGTAACCAAATCCGGAGAAAAAGTTTATAACGTCTTTTTCGAGTATTGTTCAGGGTCGAGCTTGCGTGATCACATTCTTAAATTTGGACCTAATGGGTTGCAAGATGATGAAGTTCGTAGATATACAAGAGATATCGTACATGGACTTTATTACATGCATTGTAATGGTCGATACATTCATGGTGACATAAAATCAAGAAATATTTTGCTGTCACATGGCATGGCTAAGTTGGCTAGTTTTGGATTGGCTAGGAAGCTCACTGCTGAAGTAATATGTGAGGAGGAAATTAGTGGGTCGGGACCGTACGCATCTCCGGAGTTGGCTAGAGAAGGTTATTTGGGGTGGCCTGCTGATATTTGGGCACTTGGATGCGTGGTGTTGGAGATGTTTACGGGAAAATCTGCATGGAGCTTTGAAGATGCATATAGATATCTGATGGATTCTAACAATGAGAAGATACCAGAAATTCCTAAGCACATATCAAGGGAAGGAAGAGATTTTATTAAGAAGTGTTTGATTAGAAGTCCTTATAAAAGAAGACCAATTTGGTTGCTGATCAAACATCCTTTTGTTTGCCAATGA

mRNA sequence

ATGACAAAATCCTGTGTTGTTTCACAAGTGCAAATCTCTCGCAACCTGCACGAATTCTTGAAAACAAAGAGAAGAATTCTGAAGATTGCAAAGAAATCGGCACCAACAAACAATTGGAATGACACCATCAAACATAATTATGAAAAATTTGGTGAAGATTTCACAATCGAAGAATTCCATGGCTCAATTTGGACTGGTTTTCTTCTCTTTCGTAAAACAGAATCTACCCTAATTTTCTTGGCGAAGAAGAACAGAGCACCCATTAACAACTCAAATTCAAATCTCCCTGAAGAATTTATTGTCAAATCTTCTTTAATGGAGTCCTCTTCTTCGTTACGTCATGAGAAGGAAGTGCTATCCAATCTCGGACCATTCACTAAACTAGTGGATTGTTATGGAGATGAAATTACAGTAACCAAATCCGGAGAAAAAGTTTATAACGTCTTTTTCGAGTATTGTTCAGGGTCGAGCTTGCGTGATCACATTCTTAAATTTGGACCTAATGGGTTGCAAGATGATGAAGTTCGTAGATATACAAGAGATATCGTACATGGACTTTATTACATGCATTGTAATGGTCGATACATTCATGGTGACATAAAATCAAGAAATATTTTGCTGTCACATGGCATGGCTAAGTTGGCTAGTTTTGGATTGGCTAGGAAGCTCACTGCTGAAGTAATATGTGAGGAGGAAATTAGTGGGTCGGGACCGTACGCATCTCCGGAGTTGGCTAGAGAAGGTTATTTGGGGTGGCCTGCTGATATTTGGGCACTTGGATGCGTGGTGTTGGAGATGTTTACGGGAAAATCTGCATGGAGCTTTGAAGATGCATATAGATATCTGATGGATTCTAACAATGAGAAGATACCAGAAATTCCTAAGCACATATCAAGGGAAGGAAGAGATTTTATTAAGAAGTGTTTGATTAGAAGTCCTTATAAAAGAAGACCAATTTGGTTGCTGATCAAACATCCTTTTGTTTGCCAATGA

Coding sequence (CDS)

ATGACAAAATCCTGTGTTGTTTCACAAGTGCAAATCTCTCGCAACCTGCACGAATTCTTGAAAACAAAGAGAAGAATTCTGAAGATTGCAAAGAAATCGGCACCAACAAACAATTGGAATGACACCATCAAACATAATTATGAAAAATTTGGTGAAGATTTCACAATCGAAGAATTCCATGGCTCAATTTGGACTGGTTTTCTTCTCTTTCGTAAAACAGAATCTACCCTAATTTTCTTGGCGAAGAAGAACAGAGCACCCATTAACAACTCAAATTCAAATCTCCCTGAAGAATTTATTGTCAAATCTTCTTTAATGGAGTCCTCTTCTTCGTTACGTCATGAGAAGGAAGTGCTATCCAATCTCGGACCATTCACTAAACTAGTGGATTGTTATGGAGATGAAATTACAGTAACCAAATCCGGAGAAAAAGTTTATAACGTCTTTTTCGAGTATTGTTCAGGGTCGAGCTTGCGTGATCACATTCTTAAATTTGGACCTAATGGGTTGCAAGATGATGAAGTTCGTAGATATACAAGAGATATCGTACATGGACTTTATTACATGCATTGTAATGGTCGATACATTCATGGTGACATAAAATCAAGAAATATTTTGCTGTCACATGGCATGGCTAAGTTGGCTAGTTTTGGATTGGCTAGGAAGCTCACTGCTGAAGTAATATGTGAGGAGGAAATTAGTGGGTCGGGACCGTACGCATCTCCGGAGTTGGCTAGAGAAGGTTATTTGGGGTGGCCTGCTGATATTTGGGCACTTGGATGCGTGGTGTTGGAGATGTTTACGGGAAAATCTGCATGGAGCTTTGAAGATGCATATAGATATCTGATGGATTCTAACAATGAGAAGATACCAGAAATTCCTAAGCACATATCAAGGGAAGGAAGAGATTTTATTAAGAAGTGTTTGATTAGAAGTCCTTATAAAAGAAGACCAATTTGGTTGCTGATCAAACATCCTTTTGTTTGCCAATGA

Protein sequence

MTKSCVVSQVQISRNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFHGSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ*

Homology

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BLAST of Chy4G082120 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.4e-43
Identity = 92/248 (37.10%), Postives = 150/248 (60.48%), Query Frame = 0


Query: 89  NNSNSNLPEEFIVKSSLMESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVTKSGEKVYNV 148
           + ++ + P    VKS+    ++SL +EK VL +LG   +++ CYG++ TV ++GE+++N+
Sbjct: 23  SRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGEDSTV-ENGEEMHNL 82

Query: 149 FFEYCSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILL- 208
             EY S  SL  ++ K G  GL +  VRR+T  ++ GL ++H  G + H DIK  NILL 
Sbjct: 83  LLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG-FAHCDIKLANILLF 142

Query: 209 SHGMAKLASFGLARKLTAEVIC---EEEISGSGPYASPELAREGYLGWPADIWALGCVVL 268
           + G  K+A FGLA ++  ++       EI G+  Y +PE   +   G  AD+WALGC V+
Sbjct: 143 NDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVV 202

Query: 269 EMFTGKSAWSFEDAYRY----LMDSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIW 328
           EMF+GK+AWS ++   +    +     +++P+IP+ +S EG+DF+ KC ++ P KR    
Sbjct: 203 EMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAE 262

[Close]

BLAST of Chy4G082120 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 4.7e-31
Identity = 84/225 (37.33%), Postives = 124/225 (55.11%), Query Frame = 0


Query: 107 ESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFG 166
           ES+  L  E  VLS L     +V  YG E       +K+Y ++ EY SG S+   + ++G
Sbjct: 442 ESAQQLGQEISVLSRL-RHQNIVQYYGSETV----DDKLY-IYLEYVSGGSIYKLLQEYG 501

Query: 167 PNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILLS-HGMAKLASFGLARKLTA 226
             G  ++ +R YT+ I+ GL Y+H     +H DIK  NIL+  HG  K+A FG+A+ +TA
Sbjct: 502 QFG--ENAIRNYTQQILSGLAYLHAKNT-VHRDIKGANILVDPHGRVKVADFGMAKHITA 561

Query: 227 EVICEEEISGSGPYASPELAREGY-LGWPADIWALGCVVLEMFTGKSAWS-FEDAYRYLM 286
           +        GS  + +PE+ +         DIW+LGC VLEM T K  WS +E       
Sbjct: 562 Q-SGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFK 621

Query: 287 DSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 329
             N++++P+IP H+S EG+DF++KCL R+P  R     L+ H FV
Sbjct: 622 IGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

[Close]

BLAST of Chy4G082120 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.4e-30
Identity = 83/243 (34.16%), Postives = 134/243 (55.14%), Query Frame = 0


Query: 94  NLPEEFIVKSSLMESSSSLRHEKEVLSNL-GPFTKLVDCYGDEITVTKSGEKVYNVFFEY 153
           N  E   VKSS +  S  L+ E ++LS+L  P+  ++   G E     +G  +YN+  EY
Sbjct: 24  NSDEILAVKSSEVHRSEFLQREAKILSSLSSPY--VIGYRGSETKRESNGVVMYNLLMEY 83

Query: 154 CSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSH-GM 213
               +L D   K G   + +  V +YTRDI+ GL Y+H  G  +H D+K  N+++S  G 
Sbjct: 84  APYGTLTDAAAKDG-GRVDETRVVKYTRDILKGLEYIHSKG-IVHCDVKGSNVVISEKGE 143

Query: 214 AKLASFGLARKLTAEVICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKS 273
           AK+A FG A+++  + + E  + G+  + +PE+AR    G  +DIWA+GC ++EM TG  
Sbjct: 144 AKIADFGCAKRV--DPVFESPVMGTPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSP 203

Query: 274 AWSFEDAYR------YLMDSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKH 329
            W+  D+        Y +  ++E  PE+P  ++ E +DF++KCL R   +R     L+ H
Sbjct: 204 PWTKADSREDPVSVLYRVGYSSE-TPELPCLLAEEAKDFLEKCLKREANERWTATQLLNH 259

[Close]

BLAST of Chy4G082120 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 8.2e-28
Identity = 85/273 (31.14%), Postives = 138/273 (50.55%), Query Frame = 0


Query: 60  HGSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVL 119
           +GS++ G       ++  +F  K+      NS+  L    +  S  +E   SLRH+  ++
Sbjct: 181 YGSVYLGL----NKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSLRHD-NIV 240

Query: 120 SNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYT 179
             LG               T   +   +VF EY  G S+   + KFG     ++ ++ YT
Sbjct: 241 RYLG---------------TSLDQSFLSVFLEYIPGGSISSLLGKFG--AFSENVIKVYT 300

Query: 180 RDIVHGLYYMHCNGRYIHGDIKSRNILL-SHGMAKLASFGLARKLTAEVICEEEISGSGP 239
           + I+ GL ++H N   IH DIK  NIL+ + G+ KL+ FG ++  +  V   + + G+  
Sbjct: 301 KQILQGLSFLHANS-IIHRDIKGANILIDTKGIVKLSDFGCSKSFSGIVSQFKSMQGTPY 360

Query: 240 YASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFED---AYRYLMDSNNEKIPEIPK 299
           + +PE+ ++   G  +DIW+LGCV++EM T +  WS      A  Y + S+N  IP IP 
Sbjct: 361 WMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSN-SIPNIPS 420

Query: 300 HISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 329
           H+S+E  DF+  C  R P +R     L+KHPF+
Sbjct: 421 HMSQEAFDFLNLCFKRDPKERPDANQLLKHPFI 429

[Close]

BLAST of Chy4G082120 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.4e-27
Identity = 80/226 (35.40%), Postives = 120/226 (53.10%), Query Frame = 0


Query: 107 ESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFG 166
           E    L  E  +L+ L     +V  YG E++     E+  +V+ EY SG S+   +  +G
Sbjct: 256 ECLKQLNQEINLLNQL-CHPNIVQYYGSELS-----EETLSVYLEYVSGGSIHKLLKDYG 315

Query: 167 PNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILLS-HGMAKLASFGLARKLTA 226
                +  ++ YTR I+ GL Y+H     +H DIK  NIL+  +G  KLA FG+A+ +TA
Sbjct: 316 --SFTEPVIQNYTRQILAGLAYLH-GRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTA 375

Query: 227 EVICEEEISGSGPYASPE--LAREGYLGWPADIWALGCVVLEMFTGKSAWS-FEDAYRYL 286
                    GS  + +PE  +++ GY     DIW+LGC +LEM T K  WS FE      
Sbjct: 376 -FSTMLSFKGSPYWMAPEVVMSQNGYT-HAVDIWSLGCTILEMATSKPPWSQFEGVAAIF 435

Query: 287 MDSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKHPFV 329
              N++  PEIP H+S + ++FI+ CL R+P  R     L++HPF+
Sbjct: 436 KIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFL 470

[Close]

BLAST of Chy4G082120 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 9.0e-187
Identity = 320/330 (96.97%), Postives = 325/330 (98.48%), Query Frame = 0


Query: 1   MTKSCVVSQVQISRNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60
           MTKSCVVSQVQIS NLHEFLKTKRRILKIAKKSAP NNWNDTIKHNYEKFGEDFTIEEFH
Sbjct: 1   MTKSCVVSQVQISGNLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTIEEFH 60

Query: 61  GSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLS 120
           GSIWTG LLFRKTESTL+FLAKKNRAPINNSNSNLPEEF+VKSSLMESSSSLRHEKEVLS
Sbjct: 61  GSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEKEVLS 120

Query: 121 NLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTR 180
           NLGPFT LVDCYGDEITVTKSGE+VYNVFFEYCSGSSLR+HILKFGPNGLQDDEVRRYTR
Sbjct: 121 NLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVRRYTR 180

Query: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240
           DIV GLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA
Sbjct: 181 DIVRGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240

Query: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKHISRE 300
           SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPK+ISRE
Sbjct: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKNISRE 300

Query: 301 GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 331
           GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ
Sbjct: 301 GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 330

[Close]

BLAST of Chy4G082120 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.5e-178
Identity = 306/330 (92.73%), Postives = 315/330 (95.45%), Query Frame = 0


Query: 1   MTKSCVVSQVQISRNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60
           MTKSCVVS+VQ   NLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH
Sbjct: 1   MTKSCVVSRVQFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60

Query: 61  GSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLS 120
           GSIW+G  LFRKTESTLIFLAKKNRAPINNS SNLPEEF+VKSSL ESSSSL HEKEVLS
Sbjct: 61  GSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEKEVLS 120

Query: 121 NLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTR 180
           NLGPF  LVDCYGDEIT++KSGEKVYNVFFEYCSGSSLRDHI KFGPNGLQ+ EVR+YTR
Sbjct: 121 NLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVRKYTR 180

Query: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240
           DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA
Sbjct: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240

Query: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKHISRE 300
           SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSF DA+RYLMD NNEKIPEIPK+ISRE
Sbjct: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPEIPKNISRE 300

Query: 301 GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 331
           GRDFIKKCLIRSPYKRRP WLLIKHPFVCQ
Sbjct: 301 GRDFIKKCLIRSPYKRRPSWLLIKHPFVCQ 330

[Close]

BLAST of Chy4G082120 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.5e-178
Identity = 306/330 (92.73%), Postives = 315/330 (95.45%), Query Frame = 0


Query: 1   MTKSCVVSQVQISRNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60
           MTKSCVVS+VQ   NLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH
Sbjct: 1   MTKSCVVSRVQFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60

Query: 61  GSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLS 120
           GSIW+G  LFRKTESTLIFLAKKNRAPINNS SNLPEEF+VKSSL ESSSSL HEKEVLS
Sbjct: 61  GSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEKEVLS 120

Query: 121 NLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTR 180
           NLGPF  LVDCYGDEIT++KSGEKVYNVFFEYCSGSSLRDHI KFGPNGLQ+ EVR+YTR
Sbjct: 121 NLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVRKYTR 180

Query: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240
           DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA
Sbjct: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240

Query: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKHISRE 300
           SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSF DA+RYLMD NNEKIPEIPK+ISRE
Sbjct: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPEIPKNISRE 300

Query: 301 GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 331
           GRDFIKKCLIRSPYKRRP WLLIKHPFVCQ
Sbjct: 301 GRDFIKKCLIRSPYKRRPSWLLIKHPFVCQ 330

[Close]

BLAST of Chy4G082120 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.7e-124
Identity = 231/341 (67.74%), Postives = 264/341 (77.42%), Query Frame = 0


Query: 1   MTKSCVVSQVQIS----RNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60
           MTKSCVVSQV        NLHEFLKTKR I+K AKKS  + NWN+ +KHNY +FG +F I
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFG-NFAI 60

Query: 61  EEFHGSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEK 120
           EE HGS W G  L R++ S L+FLAK      N S++N   EF+VKS LMESSSSL++EK
Sbjct: 61  EECHGSFWIGDRLIRRSGSGLVFLAK------NKSSTNSNSEFLVKSCLMESSSSLQYEK 120

Query: 121 EVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVR 180
           EVL NLGP + LV CYGDEIT++KSGE++YNV FEYC GSSLRDHI+KFG NGL +  VR
Sbjct: 121 EVLCNLGPCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVR 180

Query: 181 RYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG-------MAKLASFGLARKLTAEVIC 240
           +YTRDIV GL YMHCN  YIHGDIK RNILLS         +AKLASFGLARKLT EV+C
Sbjct: 181 KYTRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLC 240

Query: 241 EEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEK 300
           +EEISGSG Y +PELA++GYL WPADIWALGCVVLEM TGK AWSFE AY YLM+ N E 
Sbjct: 241 DEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQE- 300

Query: 301 IPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 331
           IPEIP  IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV +
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

[Close]

BLAST of Chy4G082120 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 3.3e-96
Identity = 173/232 (74.57%), Postives = 192/232 (82.76%), Query Frame = 0


Query: 106 MESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKF 165
           MESSSSL++EKEVL NLGP + LV CYGDEIT+ KSGE++YNV FEYC GSSLRDHI+KF
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 166 GPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG-------MAKLASFG 225
           G NGL +  VR+YTRDIVHGL YMHCN RYIHGDIK RNILLS         +AKLASFG
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKGGCLVAKLASFG 120

Query: 226 LARKLTAEVICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDA 285
           LARKLT EV+C+EEISGSG Y +PELA++GYL WPADIWALGCVVLEM TGK AWSFE A
Sbjct: 121 LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA 180

Query: 286 YRYLMDSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 331
           Y YLMD N E IPEIP  IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV +
Sbjct: 181 YNYLMDCNQE-IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 231

[Close]

BLAST of Chy4G082120 vs. NCBI nr
Match: XP_004147483.2 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN59621.1 hypothetical protein Csa_001780 [Cucumis sativus])

HSP 1 Score: 656 bits (1693), Expect = 1.29e-237
Identity = 320/330 (96.97%), Postives = 325/330 (98.48%), Query Frame = 0


Query: 1   MTKSCVVSQVQISRNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60
           MTKSCVVSQVQIS NLHEFLKTKRRILKIAKKSAP NNWNDTIKHNYEKFGEDFTIEEFH
Sbjct: 1   MTKSCVVSQVQISGNLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTIEEFH 60

Query: 61  GSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLS 120
           GSIWTG LLFRKTESTL+FLAKKNRAPINNSNSNLPEEF+VKSSLMESSSSLRHEKEVLS
Sbjct: 61  GSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEKEVLS 120

Query: 121 NLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTR 180
           NLGPFT LVDCYGDEITVTKSGE+VYNVFFEYCSGSSLR+HILKFGPNGLQDDEVRRYTR
Sbjct: 121 NLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVRRYTR 180

Query: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240
           DIV GLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA
Sbjct: 181 DIVRGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240

Query: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKHISRE 300
           SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPK+ISRE
Sbjct: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKNISRE 300

Query: 301 GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 330
           GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ
Sbjct: 301 GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 330

[Close]

BLAST of Chy4G082120 vs. NCBI nr
Match: XP_008443462.1 (PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like [Cucumis melo] >KAA0053712.1 mitogen-activated protein kinase kinase kinase YODA-like [Cucumis melo var. makuwa] >TYK17746.1 mitogen-activated protein kinase kinase kinase YODA-like [Cucumis melo var. makuwa])

HSP 1 Score: 629 bits (1622), Expect = 8.56e-227
Identity = 306/330 (92.73%), Postives = 315/330 (95.45%), Query Frame = 0


Query: 1   MTKSCVVSQVQISRNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60
           MTKSCVVS+VQ   NLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH
Sbjct: 1   MTKSCVVSRVQFCVNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFH 60

Query: 61  GSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLS 120
           GSIW+G  LFRKTESTLIFLAKKNRAPINNS SNLPEEF+VKSSL ESSSSL HEKEVLS
Sbjct: 61  GSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEKEVLS 120

Query: 121 NLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTR 180
           NLGPF  LVDCYGDEIT++KSGEKVYNVFFEYCSGSSLRDHI KFGPNGLQ+ EVR+YTR
Sbjct: 121 NLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVRKYTR 180

Query: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240
           DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA
Sbjct: 181 DIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYA 240

Query: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKHISRE 300
           SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSF DA+RYLMD NNEKIPEIPK+ISRE
Sbjct: 241 SPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEKIPEIPKNISRE 300

Query: 301 GRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 330
           GRDFIKKCLIRSPYKRRP WLLIKHPFVCQ
Sbjct: 301 GRDFIKKCLIRSPYKRRPSWLLIKHPFVCQ 330

[Close]

BLAST of Chy4G082120 vs. NCBI nr
Match: XP_038880300.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 488 bits (1257), Expect = 2.36e-171
Identity = 255/333 (76.58%), Postives = 274/333 (82.28%), Query Frame = 0


Query: 1   MTKSCVVSQV-QISRN---LHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60
           MTKSCVVSQV Q S N   LHEFLKTKR I+K AKKS  TNNWND +KHN EKFG+DF I
Sbjct: 1   MTKSCVVSQVNQRSHNFVNLHEFLKTKRGIVKCAKKSLRTNNWNDNVKHNSEKFGKDFAI 60

Query: 61  EEFHGSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEK 120
           EE+HGSIWTG  LFRKT S+LIFLA+ NRAP N  NSNLP EF+VKS LMESSSSL+HEK
Sbjct: 61  EEYHGSIWTGDHLFRKTGSSLIFLAR-NRAPTN-PNSNLPTEFMVKSCLMESSSSLQHEK 120

Query: 121 EVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVR 180
           EVL+NLG FT LV CYGDEIT+TKSGEKVYNV FEYC GSSLRDHILKFGPNGL + EVR
Sbjct: 121 EVLNNLGSFTNLVRCYGDEITITKSGEKVYNVLFEYCYGSSLRDHILKFGPNGLPEHEVR 180

Query: 181 RYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGS 240
           RYTRDIV+G+ YMHCNGRYIHGDIKSRNIL+S   AKLASFG ARKLTAEV+CEEEI GS
Sbjct: 181 RYTRDIVYGVCYMHCNGRYIHGDIKSRNILMSPTGAKLASFGWARKLTAEVLCEEEIRGS 240

Query: 241 GPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEKIPEIPKH 300
           GPY SPELA +GYLGWPADIWALGCVVLEM TGKS              NNE+  +I   
Sbjct: 241 GPYISPELASDGYLGWPADIWALGCVVLEMLTGKSP------------RNNEEAIQIIND 300

Query: 301 ISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVC 329
           IS+EGRDFIKKCLI SPYKRRP WLLIKHPFVC
Sbjct: 301 ISKEGRDFIKKCLITSPYKRRPSWLLIKHPFVC 319

[Close]

BLAST of Chy4G082120 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 457 bits (1177), Expect = 5.59e-159
Identity = 235/341 (68.91%), Postives = 266/341 (78.01%), Query Frame = 0


Query: 1   MTKSCVVSQVQISR----NLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60
           MTKSCVVSQV        NLHEFLKTKR I+K AKKS  +NNWN+ +KHNY +FG +F I
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFG-NFAI 60

Query: 61  EEFHGSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEK 120
           EE+HGS W G  L RK+ S L+FLAK      N S++N   EF+VKS LMESSSSL++EK
Sbjct: 61  EEYHGSFWIGDRLIRKSGSGLVFLAK------NKSSTNSNPEFLVKSCLMESSSSLQYEK 120

Query: 121 EVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVR 180
           EVL NLGP + LV CYGDEIT+ KSGE++YNV FEYC GSSLRDHI+KFG NGL +  VR
Sbjct: 121 EVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVR 180

Query: 181 RYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG-------MAKLASFGLARKLTAEVIC 240
           +YTRDIVHGL YMHCN  YIHGDIK RNILLS         +AKLASFGLARKLT EV+C
Sbjct: 181 QYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLC 240

Query: 241 EEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEK 300
           +EEISGSG Y +PELA++GYL WPADIWALGCVVLEM TGK AWSFE AY YLMD N E 
Sbjct: 241 DEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQE- 300

Query: 301 IPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 330
           IPEIP  IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV +
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

[Close]

BLAST of Chy4G082120 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 451 bits (1160), Expect = 2.15e-156
Identity = 232/341 (68.04%), Postives = 264/341 (77.42%), Query Frame = 0


Query: 1   MTKSCVVSQVQISR----NLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60
           MTKSCVVSQV        NLHEFLKTKR I+K AKKS  + NWN+ +KHNY +FG +F I
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFG-NFAI 60

Query: 61  EEFHGSIWTGFLLFRKTESTLIFLAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEK 120
           EE+HGS W G  L RK+ S L+FLAK      N S++N   EF+VKS LMESSSSL++EK
Sbjct: 61  EEYHGSFWIGDRLIRKSGSGLVFLAK------NKSSTNSNSEFLVKSCLMESSSSLQYEK 120

Query: 121 EVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVR 180
           EVL NLGP + LV CYGDEIT+ KSGE++YNV FEYC GSSLRDHI+KFG NGL +  VR
Sbjct: 121 EVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVR 180

Query: 181 RYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG-------MAKLASFGLARKLTAEVIC 240
           +YTRDIV GL YMHCN  YIHGDIK RNILLS         +AKLASFGLARKLT EV+C
Sbjct: 181 KYTRDIVRGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLC 240

Query: 241 EEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEK 300
           +EEISGSG Y +PELA++GYL WPADIWALGCVVLEM TGK AWSFE AY YLM+ N E 
Sbjct: 241 DEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQE- 300

Query: 301 IPEIPKHISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 330
           IPEIP  IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV +
Sbjct: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

[Close]

BLAST of Chy4G082120 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-46
Identity = 102/246 (41.46%), Postives = 153/246 (62.20%), Query Frame = 0


Query: 90  NSNSNLPEEFIVKSSLMESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVTKSGEKVYNVF 149
           +S+   P    VKSS +  S++LR+E++VL +LG  +++V C+G+  TV ++GE++YN+F
Sbjct: 25  SSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFGEGRTV-ENGEEIYNLF 84

Query: 150 FEYCSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILL-S 209
            EY SG SL D I   G   L + EVRR+TR IV GL ++H NG + H DIK  N+L+  
Sbjct: 85  LEYASGGSLADRIKSSG-EALPEFEVRRFTRSIVKGLCHIHGNG-FTHCDIKLENVLVFG 144

Query: 210 HGMAKLASFGLARKLTAEVICEEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFT 269
            G  K++ FGLA++ + EV    EI G+  Y +PE    G    PADIWALGC V+EM +
Sbjct: 145 DGDVKISDFGLAKRRSGEVCV--EIRGTPLYMAPESVNHGEFESPADIWALGCSVVEMSS 204

Query: 270 GKSAWSFEDAYRYLMDS------NNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIWLL 329
           GK+AW  ED     + S      + +++P IP  +S EG+DF+ KC +++  +R    +L
Sbjct: 205 GKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEML 264

[Close]

BLAST of Chy4G082120 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 178.7 bits (452), Expect = 7.6e-45
Identity = 95/248 (38.31%), Postives = 149/248 (60.08%), Query Frame = 0


Query: 89  NNSNSNLPEEFIVKSSLMESSSSLRHEKEVLSNLG-PFTKLVDCYGDEITVTKSGEKVYN 148
           NN +   P    VKS+    ++SL +EK VL NLG    ++V C+G++ TV ++GE+++N
Sbjct: 24  NNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCNEIVRCFGEDRTV-ENGEEMHN 83

Query: 149 VFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILL 208
           +F EY S  SL  ++ K    G+ +  VRR+T  ++ GL ++H NG + H D+K  NILL
Sbjct: 84  LFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGLRHIHANG-FAHCDLKLGNILL 143

Query: 209 -SHGMAKLASFGLARKL--TAEVICEEEISGSGPYASPELAREGYLGWPADIWALGCVVL 268
              G  K+A FGLA+++     +    +I G+  Y +PE   +   G   D+WALGCVV+
Sbjct: 144 FGDGAVKIADFGLAKRIGDLTALNYGVQIRGTPLYMAPESVNDNEYGSEGDVWALGCVVV 203

Query: 269 EMFTGKSAWSFEDAYRY----LMDSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIW 328
           EMF+GK+AWS ++   +    L     +++P IP+ +S +GRDF+ KC ++ P KR    
Sbjct: 204 EMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAE 263

[Close]

BLAST of Chy4G082120 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 177.6 bits (449), Expect = 1.7e-44
Identity = 92/248 (37.10%), Postives = 150/248 (60.48%), Query Frame = 0


Query: 89  NNSNSNLPEEFIVKSSLMESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVTKSGEKVYNV 148
           + ++ + P    VKS+    ++SL +EK VL +LG   +++ CYG++ TV ++GE+++N+
Sbjct: 23  SRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGEDSTV-ENGEEMHNL 82

Query: 149 FFEYCSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGDIKSRNILL- 208
             EY S  SL  ++ K G  GL +  VRR+T  ++ GL ++H  G + H DIK  NILL 
Sbjct: 83  LLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG-FAHCDIKLANILLF 142

Query: 209 SHGMAKLASFGLARKLTAEVIC---EEEISGSGPYASPELAREGYLGWPADIWALGCVVL 268
           + G  K+A FGLA ++  ++       EI G+  Y +PE   +   G  AD+WALGC V+
Sbjct: 143 NDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVV 202

Query: 269 EMFTGKSAWSFEDAYRY----LMDSNNEKIPEIPKHISREGRDFIKKCLIRSPYKRRPIW 328
           EMF+GK+AWS ++   +    +     +++P+IP+ +S EG+DF+ KC ++ P KR    
Sbjct: 203 EMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAE 262

[Close]

BLAST of Chy4G082120 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 149.4 bits (376), Expect = 4.9e-36
Identity = 95/254 (37.40%), Postives = 145/254 (57.09%), Query Frame = 0


Query: 80  LAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLSNL-GPFTKLVDCYGDEITV 139
           + + + A ++   +N  + F VKS+   SS+ L+ E+ +LS L  P+  +V   G    V
Sbjct: 12  IGRGSTATVSLGITNSGDFFAVKSAEFSSSAFLQREQSILSKLSSPY--IVKYIGS--NV 71

Query: 140 TKSGEK-VYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIH 199
           TK  +K +YN+  EY SG SL D ++K     L +  +R YTR I+ GL Y+H  G  +H
Sbjct: 72  TKENDKLMYNLLMEYVSGGSLHD-LIKNSGGKLPEPLIRSYTRQILKGLMYLHDQG-IVH 131

Query: 200 GDIKSRNILLSHGMAKLASFGLARKLTAEVICEEEISGSGPYASPELAREGYLGWPADIW 259
            D+KS+N+++   +AK+   G A+  T E     E SG+  + SPE+AR     +PAD+W
Sbjct: 132 CDVKSQNVMIGGEIAKIVDLGCAK--TVEENENLEFSGTPAFMSPEVARGEEQSFPADVW 191

Query: 260 ALGCVVLEMFTGKSAW-SFED--AYRYLMDSNNEKIPEIPKHISREGRDFIKKCLIRSPY 319
           ALGC V+EM TG S W    D  A  Y +    E  P IP  +S +G+DF++KCL + P 
Sbjct: 192 ALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGES-PVIPVWLSEKGQDFLRKCLRKDPK 251

Query: 320 KRRPIWLLIKHPFV 329
           +R  +  L++HPF+
Sbjct: 252 QRWTVEELLQHPFL 256

[Close]

BLAST of Chy4G082120 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 147.5 bits (371), Expect = 1.9e-35
Identity = 93/253 (36.76%), Postives = 144/253 (56.92%), Query Frame = 0


Query: 80  LAKKNRAPINNSNSNLPEEFIVKSSLMESSSSLRHEKEVLSNLGPFTKLVDCYGDEITVT 139
           + + + A ++ + S+  E F VKS+ + SSS L+ E+ +LS L     +V   G  +T  
Sbjct: 11  IGRGSTATVSIAISSSGELFAVKSADLSSSSLLQKEQSILSTLSS-PHMVKYIGTGLTRE 70

Query: 140 KSGEKVYNVFFEYCSGSSLRDHILKFGPNGLQDDEVRRYTRDIVHGLYYMHCNGRYIHGD 199
            +G  VYN+  EY SG +L D ++K     L + E+R YTR I++GL Y+H  G  +H D
Sbjct: 71  SNG-LVYNILMEYVSGGNLHD-LIKNSGGKLPEPEIRSYTRQILNGLVYLHERG-IVHCD 130

Query: 200 IKSRNILL-SHGMAKLASFGLARKLTAEVICEEEISGSGPYASPELAREGYLGWPADIWA 259
           +KS N+L+  +G+ K+A  G A+      + + E SG+  + +PE+AR     +PAD+WA
Sbjct: 131 LKSHNVLVEENGVLKIADMGCAKS-----VDKSEFSGTPAFMAPEVARGEEQRFPADVWA 190

Query: 260 LGCVVLEMFTGKSAW-SFED--AYRYLMDSNNEKIPEIPKHISREGRDFIKKCLIRSPYK 319
           LGC ++EM TG S W    D  A  Y +  + E  P IP  IS + +DF+K CL     +
Sbjct: 191 LGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGES-PAIPAWISDKAKDFLKNCLKEDQKQ 250

Query: 320 RRPIWLLIKHPFV 329
           R  +  L+KHPF+
Sbjct: 251 RWTVEELLKHPFL 253

The following BLAST results are available for this feature:

BLAST of Chy4G082120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	2.4e-43	37.10	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q9CAD5	4.7e-31	37.33	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3...	[more]
O80888	1.4e-30	34.16	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q54R82	8.2e-28	31.14	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
F4HRJ4	2.4e-27	35.40	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Chy4G082120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHV1	9.0e-187	96.97	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829...	[more]
A0A5D3D3K9	1.5e-178	92.73	Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma...	[more]
A0A1S3B829	1.5e-178	92.73	mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656...	[more]
A0A6J1HBP5	3.7e-124	67.74	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A6J1JAH8	3.3e-96	74.57	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36...	[more]

BLAST of Chy4G082120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147483.2	1.29e-237	96.97	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN59621.1 ...	[more]
XP_008443462.1	8.56e-227	92.73	PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like [Cucumis mel...	[more]
XP_038880300.1	2.36e-171	76.58	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_023516554.1	5.59e-159	68.91	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe...	[more]
KAG7023508.1	2.15e-156	68.04	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]

BLAST of Chy4G082120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	1.8e-46	41.46	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G67080.1	7.6e-45	38.31	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G50310.1	1.7e-44	37.10	mitogen-activated protein kinase kinase kinase 20	[more]
AT5G55090.1	4.9e-36	37.40	mitogen-activated protein kinase kinase kinase 15	[more]
AT4G26890.1	1.9e-35	36.76	mitogen-activated protein kinase kinase kinase 16	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 71..328 e-value: 2.4E-34 score: 130.1
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 109..328 e-value: 1.6E-36 score: 126.1
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 54..328 score: 31.085625
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 93..330 e-value: 9.5E-47 score: 161.7
None	No IPR available	PIRSR	PIRSR000620-2	PIRSR000620-2	coord: 174..321 e-value: 6.9E-7 score: 26.4
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 46..328
None	No IPR available	PANTHER	PTHR48011:SF55	SUBFAMILY NOT NAMED	coord: 46..328
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 195..207
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 98..329

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G082120.1	Chy4G082120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity