Chy4G080600 (gene) Cucumber (hystrix) v1

Overview
NameChy4G080600
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationchrH04: 20031321 .. 20032658 (+)
RNA-Seq ExpressionChy4G080600
SyntenyChy4G080600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGTGGGGCGGCGCAGGTTAGGAATGTTCTTTTTGGGAAATACGAAATGGGTCGGCTTTTGGGGAAAGGAACCTTTGCGAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCCATTAAAGTGATTCTCAAGGACAGGGTGAAGAAAAAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGGTTGGTTCGTCATCCTCATATTGTGGAATTGAAAGAAGTTATGGCGACGAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAGTTGTCTCAACTAGGGAAATTGACTGAGGATGGAGCGAGGAATTATTTTCAACAGCTTATCTCTGCCGTGGACTTTTGCCATAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAGAATCTCCTTCTTGACGTGAATGGGAATTTGAAGGTTTCTGATTTTGGTCTCTCTGCCCTGCCGGAGCAGCTACGGATTGATGGTTTGCTTCACACACGGTGTGGAACTCCGGCTTATGTGGCGCCTGAGGTGCTCAGAAAGAGAGGATACGATGGCGCTAAGGCGGATATCTGGTCTTGCGGGGTCATTTTGTTTGTGTTGCTTGCTGGGACTTTGCCATTTCGAGCTGATAATGCGATGAAGTTGTATAGGAAGATTTTCAAGGCGGAGTTTGAGTTCCCTCCATGGTTTTCTTCAGAAGCCAAGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGGTTGAGATAATGCATAGTCCTTGGTTTCAAAAGGGCTTCACAAAGCCAGTTTCATTCATTGAGAAAGATGAATTCAACAATGAATTCTCTGAATTGGCTAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTTTACAATGCATTTGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACTTGTCTAGTTTGTTTGAAAGCAAGAAGAAACAACGCTCTATTTTCACTTCAAAATGCCCTGCTTCAGCCATTTTAGCCAAGCTTCAATCTCTGGCCGTGAAGCTCAACTTCAAAGCTACTAACTTAAAGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACAGTGACCGCAGAGGTTTACGAGGTGGCACCGGAGCTGGCAGTCGTAGAGTTCTCGAAGTCGGCGGGCGACACTTTGGAATACAACAAGTTATGTGAGAAGGATGTGAGGCCCGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAAAACGAAGTGCCTTTAACAGTGGATTCTATGCAAAATGACCATTGA

mRNA sequence

ATGGCGGGTGGGGCGGCGCAGGTTAGGAATGTTCTTTTTGGGAAATACGAAATGGGTCGGCTTTTGGGGAAAGGAACCTTTGCGAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCCATTAAAGTGATTCTCAAGGACAGGGTGAAGAAAAAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGGTTGGTTCGTCATCCTCATATTGTGGAATTGAAAGAAGTTATGGCGACGAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAGTTGTCTCAACTAGGGAAATTGACTGAGGATGGAGCGAGGAATTATTTTCAACAGCTTATCTCTGCCGTGGACTTTTGCCATAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAGAATCTCCTTCTTGACGTGAATGGGAATTTGAAGGTTTCTGATTTTGGTCTCTCTGCCCTGCCGGAGCAGCTACGGATTGATGGTTTGCTTCACACACGGTGTGGAACTCCGGCTTATGTGGCGCCTGAGGTGCTCAGAAAGAGAGGATACGATGGCGCTAAGGCGGATATCTGGTCTTGCGGGGTCATTTTGTTTGTGTTGCTTGCTGGGACTTTGCCATTTCGAGCTGATAATGCGATGAAGTTGTATAGGAAGATTTTCAAGGCGGAGTTTGAGTTCCCTCCATGGTTTTCTTCAGAAGCCAAGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGGTTGAGATAATGCATAGTCCTTGGTTTCAAAAGGGCTTCACAAAGCCAGTTTCATTCATTGAGAAAGATGAATTCAACAATGAATTCTCTGAATTGGCTAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTTTACAATGCATTTGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACTTGTCTAGTTTGTTTGAAAGCAAGAAGAAACAACGCTCTATTTTCACTTCAAAATGCCCTGCTTCAGCCATTTTAGCCAAGCTTCAATCTCTGGCCGTGAAGCTCAACTTCAAAGCTACTAACTTAAAGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACAGTGACCGCAGAGGTTTACGAGGTGGCACCGGAGCTGGCAGTCGTAGAGTTCTCGAAGTCGGCGGGCGACACTTTGGAATACAACAAGTTATGTGAGAAGGATGTGAGGCCCGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAAAACGAAGTGCCTTTAACAGTGGATTCTATGCAAAATGACCATTGA

Coding sequence (CDS)

ATGGCGGGTGGGGCGGCGCAGGTTAGGAATGTTCTTTTTGGGAAATACGAAATGGGTCGGCTTTTGGGGAAAGGAACCTTTGCGAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCCATTAAAGTGATTCTCAAGGACAGGGTGAAGAAAAAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGGTTGGTTCGTCATCCTCATATTGTGGAATTGAAAGAAGTTATGGCGACGAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAGTTGTCTCAACTAGGGAAATTGACTGAGGATGGAGCGAGGAATTATTTTCAACAGCTTATCTCTGCCGTGGACTTTTGCCATAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAGAATCTCCTTCTTGACGTGAATGGGAATTTGAAGGTTTCTGATTTTGGTCTCTCTGCCCTGCCGGAGCAGCTACGGATTGATGGTTTGCTTCACACACGGTGTGGAACTCCGGCTTATGTGGCGCCTGAGGTGCTCAGAAAGAGAGGATACGATGGCGCTAAGGCGGATATCTGGTCTTGCGGGGTCATTTTGTTTGTGTTGCTTGCTGGGACTTTGCCATTTCGAGCTGATAATGCGATGAAGTTGTATAGGAAGATTTTCAAGGCGGAGTTTGAGTTCCCTCCATGGTTTTCTTCAGAAGCCAAGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGGTTGAGATAATGCATAGTCCTTGGTTTCAAAAGGGCTTCACAAAGCCAGTTTCATTCATTGAGAAAGATGAATTCAACAATGAATTCTCTGAATTGGCTAATCCCAAGTTGTTGTCATCTTCTCCTCCTTTTTACAATGCATTTGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACTTGTCTAGTTTGTTTGAAAGCAAGAAGAAACAACGCTCTATTTTCACTTCAAAATGCCCTGCTTCAGCCATTTTAGCCAAGCTTCAATCTCTGGCCGTGAAGCTCAACTTCAAAGCTACTAACTTAAAGGAGTTCAAGGTGAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACAGTGACCGCAGAGGTTTACGAGGTGGCACCGGAGCTGGCAGTCGTAGAGTTCTCGAAGTCGGCGGGCGACACTTTGGAATACAACAAGTTATGTGAGAAGGATGTGAGGCCCGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAAAACGAAGTGCCTTTAACAGTGGATTCTATGCAAAATGACCATTGA

Protein sequence

MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQIRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPALKDIVWSWQGENEVPLTVDSMQNDH*
Homology
BLAST of Chy4G080600 vs. ExPASy Swiss-Prot
Match: Q8W1D5 (CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CIPK25 PE=2 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.1e-167
Identity = 295/434 (67.97%), Postives = 353/434 (81.34%), Query Frame = 0

Query: 7   QVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQIRREIS 66
           Q   VLF KYEMGRLLGKGTF KVYYG+ + T ESVAIK+I KD+VK++G++EQI+REIS
Sbjct: 34  QQLRVLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREIS 93

Query: 67  VMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISA 126
           +M LVRHP+IVELKEVMATK+K++F+MEYV GGELF K+ + GKL ED AR YFQQLISA
Sbjct: 94  IMRLVRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKIVK-GKLKEDSARKYFQQLISA 153

Query: 127 VDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPE 186
           VDFCHSRGVSHRDLKPENLL+D NG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPE
Sbjct: 154 VDFCHSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPE 213

Query: 187 VLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKE 246
           VLRK+GYDGAK DIWSCG+IL+VLLAG LPF+ +N MK+YRKIFK+EFE+PPWFS E+K 
Sbjct: 214 VLRKKGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKR 273

Query: 247 MVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSF-IEKDEFNNEFSE-------LANPK 306
           ++SKLLVVDP KR S+  IM +PWF+K    P+ F I++ E  N   E        A   
Sbjct: 274 LISKLLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTT 333

Query: 307 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNF 366
               SP F+NAFEFISSMSSGFDLSSLFESK+K RS+FTS+  AS I+ KL+ +  ++N 
Sbjct: 334 TTPVSPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNM 393

Query: 367 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVR 426
           K    K+FKV+L  K EGRKG++ VTAEV+EVAPE+AVVE  KSAGDTLEYN+L E+ VR
Sbjct: 394 KVKRTKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVR 453

Query: 427 PALKDIVWSWQGEN 433
           PAL++IVWSW G+N
Sbjct: 454 PALEEIVWSWHGDN 466

BLAST of Chy4G080600 vs. ExPASy Swiss-Prot
Match: Q9LEU7 (CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CIPK5 PE=1 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 1.5e-165
Identity = 289/434 (66.59%), Postives = 359/434 (82.72%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKKGLLEQIRREISV 68
           R VLFGKYEMGRLLGKGTFAKVYYG+ ++  E VAIKVI KD+V K+ G++EQI+REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S+ GKL ED AR YFQQLISAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKISK-GKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLD NG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKEM 248
           L+K+GYDGAKADIWSCGV+L+VLLAG LPF+ +N M +YRKIF+A+FEFPPWFS EA+ +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSF------IEKDEFNNEFSEL---ANPK 308
           +SKLLVVDP +R S+  IM +PW +K FT P++F        +   NNE  E       +
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNF 368
               SP F+NAFEFISSMSSGFDLSSLFESK+K +S+FTS+  A+ ++ K++++  ++N 
Sbjct: 305 TEPISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTKEMNM 364

Query: 369 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVR 428
           K    K+FKV+++ K EGRKG+L++TAEV+EVAPE++VVEF KSAGDTLEY++L E++VR
Sbjct: 365 KVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYEEEVR 424

Query: 429 PALKDIVWSWQGEN 433
           PAL DIVWSW G+N
Sbjct: 425 PALNDIVWSWHGDN 437

BLAST of Chy4G080600 vs. ExPASy Swiss-Prot
Match: Q9SEZ7 (CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=3702 GN=CIPK16 PE=1 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.4e-147
Identity = 268/439 (61.05%), Postives = 338/439 (76.99%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKKGLLEQIRREISVMG 70
           VLF KY +GRLLG G FAKVY+G  + T + VAIKVI KD V K++G++EQI REI+VM 
Sbjct: 10  VLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMR 69

Query: 71  LVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDF 130
           L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED AR YFQQLISAVDF
Sbjct: 70  LLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDF 129

Query: 131 CHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSAL--PEQL-----RIDGLLHTRCGTPAY 190
           CHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHTRCGTPAY
Sbjct: 130 CHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAY 189

Query: 191 VAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSS 250
           VAPEVLR +GYDGA ADIWSCG++L+ LLAG LPF  +N M LY KIFKAE EFPPWFS 
Sbjct: 190 VAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSL 249

Query: 251 EAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEF--------NNEFSEL 310
           E+KE++S+LLV DP++R SM EI   PWF+K FT  V+F   +            +  +L
Sbjct: 250 ESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDL 309

Query: 311 ANPKLLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAV 370
              +   +SP  +NAF+FI+SMSSGFDLS+LFE K+K + +FTSK PA ++  +L++ A 
Sbjct: 310 NEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAR 369

Query: 371 KLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCE 430
           +++ +  ++K+ K++LQ + EGRKG+L+VTAEV+EVAPE++VVEF K++GDTLEY   CE
Sbjct: 370 EMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCE 429

Query: 431 KDVRPALKDIVWSWQGENE 434
            DVRPALKDIVWSWQG+++
Sbjct: 430 DDVRPALKDIVWSWQGDDD 448

BLAST of Chy4G080600 vs. ExPASy Swiss-Prot
Match: Q6ERS4 (CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK16 PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.5e-144
Identity = 250/426 (58.69%), Postives = 335/426 (78.64%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKK-KGLLEQIRREISV 68
           R ++ G+YE+GRLLG+GTFAKVYYGR+L + ESVAIKVI K R+++ +G++EQ+RREIS+
Sbjct: 15  RKLVLGRYELGRLLGQGTFAKVYYGRDLRSGESVAIKVIDKARLRRTEGMVEQLRREISI 74

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAV 128
           M +VRHP++V ++EV+A++++V+ VMEY  GGELF K+++ G+LTE+ AR YFQQL++AV
Sbjct: 75  MRMVRHPNVVGIREVLASRARVFVVMEYARGGELFAKVAR-GRLTEEHARRYFQQLVAAV 134

Query: 129 DFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
            FCH RGV+HRDLKPENLLLD  G LKV+DFGL+ALPEQLR DGLLHT+CGTPAYVAPEV
Sbjct: 135 GFCHGRGVAHRDLKPENLLLDEEGRLKVTDFGLAALPEQLRQDGLLHTQCGTPAYVAPEV 194

Query: 189 LRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKEM 248
           LRKRGYDGA+AD+WSCGV+L+VLL G LPF+ +N  K+Y+KIFKAE++ PPW S +A+ +
Sbjct: 195 LRKRGYDGARADLWSCGVVLYVLLCGFLPFQHENYAKMYQKIFKAEYQVPPWVSGDARRL 254

Query: 249 VSKLLVVDPQKRASMVEIMHSPWFQKGFTKPV--SFIEKDEFNNEFSELANPKLLSSSPP 308
           + +LLVVDP KR S+ EIM +PWF+KGF  PV  S +   ++  +   L      + SP 
Sbjct: 255 IVRLLVVDPAKRISIPEIMRTPWFKKGFVPPVPTSPVSPKKWEEDDVLLDGGDSGAMSPR 314

Query: 309 FYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKATNLKE 368
             NAF+ ISSMSSGFDLS +FES++K  ++FTS+ PA+ ++ KL+++   L + AT  K 
Sbjct: 315 TCNAFQLISSMSSGFDLSGMFESEQKAATVFTSRAPAATVIQKLEAVGRSLGYSATRGKG 374

Query: 369 FKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPALKDIV 428
           +K+RL+A  +G  G+L VT E  EVA ++AVVEF+  AGD LE+NK C  DVRP L DIV
Sbjct: 375 WKLRLEATADGANGRLAVTVEALEVAADVAVVEFAHDAGDELEFNKFCAVDVRPGLADIV 434

Query: 429 WSWQGE 432
           W+WQG+
Sbjct: 435 WAWQGD 439

BLAST of Chy4G080600 vs. ExPASy Swiss-Prot
Match: Q9FJ54 (CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CIPK20 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 5.2e-134
Identity = 243/425 (57.18%), Postives = 311/425 (73.18%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQIRREISVMGL 70
           VL  KYE+GRLLG+GTFAKVY+ RN+ T ESVAIKVI K +V K GL++QI+REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S+ GKL E+ AR YFQQLI A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSK-GKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLD NG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 RGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GYDGAKAD+WSCGV+L+VLLAG LPF   N +++YRKI K EF+ P WF  E K+++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPQKRASMVEIMHSPWFQKGFTK---PVSFIEKDEFNNEFSELANPKLLSSSPPFY 310
           +L  +P  R  + +IM + WFQKGF K   P S  E  + ++  S++      S  P  Y
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV--HAAFSVKPMSY 306

Query: 311 NAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKATNLKEFK 370
           NAF+ ISS+S GFDLS LFE +++  S FT+K  A  I++K + +A           +  
Sbjct: 307 NAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVG 366

Query: 371 VRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPALKDIVWS 430
           V+++ K EGRKG L +  E++EV     +VEF KS GDT+EY + C++++RP+LKDIVW 
Sbjct: 367 VKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWK 426

Query: 431 WQGEN 433
           WQG N
Sbjct: 427 WQGNN 427

BLAST of Chy4G080600 vs. ExPASy TrEMBL
Match: A0A5A7T8B4 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00290 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.8e-246
Identity = 432/445 (97.08%), Postives = 440/445 (98.88%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AAQVRNVLFGKYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQ+GFTKPVSF+EKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQ SIFTSKCPASAI+AKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV+EVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENEVPLTVDSMQNDH 446
           LKDIVWSWQGENEVPLTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENEVPLTVDSLQNDH 445

BLAST of Chy4G080600 vs. ExPASy TrEMBL
Match: A0A1S3CN52 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103502821 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 6.8e-246
Identity = 432/445 (97.08%), Postives = 440/445 (98.88%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AAQVRNVLFGKYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQ+GFTKPVSF+EKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQ SIFTSKCPASAI+AKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV+EVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENEVPLTVDSMQNDH 446
           LKDIVWSWQGENEVPLTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENEVPLTVDSLQNDH 445

BLAST of Chy4G080600 vs. ExPASy TrEMBL
Match: A0A6J1F2J4 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441609 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.6e-223
Identity = 393/433 (90.76%), Postives = 411/433 (94.92%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AA  RNVLFGKYEMGRLLGKGTFAKVY+G NLMTH+SVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDE N+E S+L NPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVYEVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 434
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of Chy4G080600 vs. ExPASy TrEMBL
Match: A0A6J1J0B3 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111481539 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 4.0e-222
Identity = 392/433 (90.53%), Postives = 410/433 (94.69%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AA  RNVLFGKYEMGRLLGKGTFAKVY+GRNLMTH+SVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVME VNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMECVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDEF +E SELANPK+L
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDEFKSEISELANPKML 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQA EEGRKGKL VT EVYEVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAMEEGRKGKLAVTMEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 434
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of Chy4G080600 vs. ExPASy TrEMBL
Match: A0A6J1DJA1 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020996 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.3e-216
Identity = 381/433 (87.99%), Postives = 405/433 (93.53%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AA  RNVLFGKYEMGRLLGKGTFAKVYYGRNL+THESVAIKVILK+RVKK+GLL+Q
Sbjct: 1   MAGEAAPSRNVLFGKYEMGRLLGKGTFAKVYYGRNLVTHESVAIKVILKERVKKEGLLDQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           I+REISVMGLVRHPHIVELKEVMATKSKVYFVMEYV+GGELFVKLS+ GKLTED AR YF
Sbjct: 61  IQREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVSGGELFVKLSKQGKLTEDAARKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           +QL+SAVDFCHSRGVSHRDLKPENLL+D NGNLKVSDFGLSALPEQLR+DGLLHTRCGTP
Sbjct: 121 RQLVSAVDFCHSRGVSHRDLKPENLLVDENGNLKVSDFGLSALPEQLRVDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGYDGAK DIWSCGVIL+VLLAG LPFRADN MKLYRKIFKA+FEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKVDIWSCGVILYVLLAGALPFRADNVMKLYRKIFKADFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSE+ EM+SK LVVDP+ R SM EIM SPWF+ GF+KP+SF EKDEFNNE SEL NPKL 
Sbjct: 241 SSESMEMISKFLVVDPENRVSMPEIMQSPWFRNGFSKPISFSEKDEFNNEISELGNPKL- 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
            SSPPFYNAFEFIS+MSSGFDLSSLFESKKK  SIFTSKC ASAI+AKLQSLA K+NFKA
Sbjct: 301 -SSPPFYNAFEFISTMSSGFDLSSLFESKKKSGSIFTSKCSASAIVAKLQSLAQKVNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNLKE KVRLQAKEEGRKGKLTVTAEV+EVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLKECKVRLQAKEEGRKGKLTVTAEVFEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 434
           LKDIVWSWQGE+E
Sbjct: 421 LKDIVWSWQGESE 431

BLAST of Chy4G080600 vs. NCBI nr
Match: XP_004137659.1 (CBL-interacting serine/threonine-protein kinase 25 [Cucumis sativus])

HSP 1 Score: 865 bits (2236), Expect = 0.0
Identity = 438/445 (98.43%), Postives = 442/445 (99.33%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAGGAAQVRNVLF KYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQ SIFTSKCPASAILAKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELA+VEFSKSAGDTLEYNKLCEKDVRPA
Sbjct: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420

Query: 421 LKDIVWSWQGENEVPLTVDSMQNDH 445
           LKDIVWSWQGE+EVPLTVDSMQNDH
Sbjct: 421 LKDIVWSWQGESEVPLTVDSMQNDH 445

BLAST of Chy4G080600 vs. NCBI nr
Match: XP_008465153.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo] >KAA0037705.1 CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo var. makuwa] >TYK27019.1 CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo var. makuwa])

HSP 1 Score: 857 bits (2213), Expect = 5.63e-313
Identity = 432/445 (97.08%), Postives = 440/445 (98.88%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AAQVRNVLFGKYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGARNYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQ+GFTKPVSF+EKDEFNNEFSELANPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQ SIFTSKCPASAI+AKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV+EVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENEVPLTVDSMQNDH 445
           LKDIVWSWQGENEVPLTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENEVPLTVDSLQNDH 445

BLAST of Chy4G080600 vs. NCBI nr
Match: XP_038905015.1 (CBL-interacting serine/threonine-protein kinase 25-like [Benincasa hispida])

HSP 1 Score: 814 bits (2103), Expect = 3.14e-296
Identity = 415/445 (93.26%), Postives = 423/445 (95.06%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG A    NVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGEAPPRMNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGAR YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GYDGAKADIWSCGVILFVLLAGTLPFRADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEA EMVSKLLVVDPQKRASMVEIM SPWFQKGFTKPVSF EKDEF+NEFSELANPK L
Sbjct: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFAEKDEFSNEFSELANPKFL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SS PPFYNAFEFISSMSSGFDLSSLFESKK++ SIFTSKC  SAI+AKLQSLAVKLNFKA
Sbjct: 301 SS-PPFYNAFEFISSMSSGFDLSSLFESKKRRGSIFTSKCSPSAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKL VTAEVYEVAPELAVVEFSKS GDTLEYNK CEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLAVTAEVYEVAPELAVVEFSKSTGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENEVPLTVDSMQNDH 445
           LKDIVWSWQGENE  LTVDS+QNDH
Sbjct: 421 LKDIVWSWQGENECLLTVDSLQNDH 444

BLAST of Chy4G080600 vs. NCBI nr
Match: KAG6581626.1 (CBL-interacting serine/threonine-protein kinase 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 782 bits (2020), Expect = 9.89e-284
Identity = 393/433 (90.76%), Postives = 411/433 (94.92%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AA  RNVLFGKYEMGRLLGKGTFAKVY+GRNLMTH+SVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDE N+E S+L NPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVYEVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 433
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of Chy4G080600 vs. NCBI nr
Match: XP_022934429.1 (CBL-interacting serine/threonine-protein kinase 25-like [Cucurbita moschata])

HSP 1 Score: 781 bits (2018), Expect = 1.99e-283
Identity = 393/433 (90.76%), Postives = 411/433 (94.92%), Query Frame = 0

Query: 1   MAGGAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQ 60
           MAG AA  RNVLFGKYEMGRLLGKGTFAKVY+G NLMTH+SVAIKVILKDRVKK+GLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDG R YF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKRGY+GAK+D+WSCGVILFVLLAG+LPF+ADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300
           SSEAKEMVSKLLVVDP+KRASM EIM S WFQKGFTKP+SF +KDE N+E S+L NPKLL
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKA 360
           SSSPPFYNAFEFISSMSSGFDL+SLFESKKKQ SIFTSKC ASAI+AKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVYEVAPELAVVEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 433
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of Chy4G080600 vs. TAIR 10
Match: AT5G25110.1 (CBL-interacting protein kinase 25 )

HSP 1 Score: 590.1 bits (1520), Expect = 1.5e-168
Identity = 295/434 (67.97%), Postives = 353/434 (81.34%), Query Frame = 0

Query: 7   QVRNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQIRREIS 66
           Q   VLF KYEMGRLLGKGTF KVYYG+ + T ESVAIK+I KD+VK++G++EQI+REIS
Sbjct: 34  QQLRVLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREIS 93

Query: 67  VMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISA 126
           +M LVRHP+IVELKEVMATK+K++F+MEYV GGELF K+ + GKL ED AR YFQQLISA
Sbjct: 94  IMRLVRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKIVK-GKLKEDSARKYFQQLISA 153

Query: 127 VDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPE 186
           VDFCHSRGVSHRDLKPENLL+D NG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPE
Sbjct: 154 VDFCHSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPE 213

Query: 187 VLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKE 246
           VLRK+GYDGAK DIWSCG+IL+VLLAG LPF+ +N MK+YRKIFK+EFE+PPWFS E+K 
Sbjct: 214 VLRKKGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKR 273

Query: 247 MVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSF-IEKDEFNNEFSE-------LANPK 306
           ++SKLLVVDP KR S+  IM +PWF+K    P+ F I++ E  N   E        A   
Sbjct: 274 LISKLLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTT 333

Query: 307 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNF 366
               SP F+NAFEFISSMSSGFDLSSLFESK+K RS+FTS+  AS I+ KL+ +  ++N 
Sbjct: 334 TTPVSPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNM 393

Query: 367 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVR 426
           K    K+FKV+L  K EGRKG++ VTAEV+EVAPE+AVVE  KSAGDTLEYN+L E+ VR
Sbjct: 394 KVKRTKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVR 453

Query: 427 PALKDIVWSWQGEN 433
           PAL++IVWSW G+N
Sbjct: 454 PALEEIVWSWHGDN 466

BLAST of Chy4G080600 vs. TAIR 10
Match: AT5G10930.1 (CBL-interacting protein kinase 5 )

HSP 1 Score: 583.9 bits (1504), Expect = 1.1e-166
Identity = 289/434 (66.59%), Postives = 359/434 (82.72%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKKGLLEQIRREISV 68
           R VLFGKYEMGRLLGKGTFAKVYYG+ ++  E VAIKVI KD+V K+ G++EQI+REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S+ GKL ED AR YFQQLISAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKISK-GKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLD NG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKEM 248
           L+K+GYDGAKADIWSCGV+L+VLLAG LPF+ +N M +YRKIF+A+FEFPPWFS EA+ +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSF------IEKDEFNNEFSEL---ANPK 308
           +SKLLVVDP +R S+  IM +PW +K FT P++F        +   NNE  E       +
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 LLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNF 368
               SP F+NAFEFISSMSSGFDLSSLFESK+K +S+FTS+  A+ ++ K++++  ++N 
Sbjct: 305 TEPISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTKEMNM 364

Query: 369 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVR 428
           K    K+FKV+++ K EGRKG+L++TAEV+EVAPE++VVEF KSAGDTLEY++L E++VR
Sbjct: 365 KVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYEEEVR 424

Query: 429 PALKDIVWSWQGEN 433
           PAL DIVWSW G+N
Sbjct: 425 PALNDIVWSWHGDN 437

BLAST of Chy4G080600 vs. TAIR 10
Match: AT2G25090.1 (CBL-interacting protein kinase 16 )

HSP 1 Score: 523.5 bits (1347), Expect = 1.7e-148
Identity = 268/439 (61.05%), Postives = 338/439 (76.99%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKKGLLEQIRREISVMG 70
           VLF KY +GRLLG G FAKVY+G  + T + VAIKVI KD V K++G++EQI REI+VM 
Sbjct: 10  VLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMR 69

Query: 71  LVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDF 130
           L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED AR YFQQLISAVDF
Sbjct: 70  LLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDF 129

Query: 131 CHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSAL--PEQL-----RIDGLLHTRCGTPAY 190
           CHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHTRCGTPAY
Sbjct: 130 CHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAY 189

Query: 191 VAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSS 250
           VAPEVLR +GYDGA ADIWSCG++L+ LLAG LPF  +N M LY KIFKAE EFPPWFS 
Sbjct: 190 VAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSL 249

Query: 251 EAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEF--------NNEFSEL 310
           E+KE++S+LLV DP++R SM EI   PWF+K FT  V+F   +            +  +L
Sbjct: 250 ESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDL 309

Query: 311 ANPKLLSSSPPFYNAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAV 370
              +   +SP  +NAF+FI+SMSSGFDLS+LFE K+K + +FTSK PA ++  +L++ A 
Sbjct: 310 NEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAR 369

Query: 371 KLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCE 430
           +++ +  ++K+ K++LQ + EGRKG+L+VTAEV+EVAPE++VVEF K++GDTLEY   CE
Sbjct: 370 EMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCE 429

Query: 431 KDVRPALKDIVWSWQGENE 434
            DVRPALKDIVWSWQG+++
Sbjct: 430 DDVRPALKDIVWSWQGDDD 448

BLAST of Chy4G080600 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 479.2 bits (1232), Expect = 3.7e-135
Identity = 243/425 (57.18%), Postives = 311/425 (73.18%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQIRREISVMGL 70
           VL  KYE+GRLLG+GTFAKVY+ RN+ T ESVAIKVI K +V K GL++QI+REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S+ GKL E+ AR YFQQLI A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSK-GKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLD NG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 RGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GYDGAKAD+WSCGV+L+VLLAG LPF   N +++YRKI K EF+ P WF  E K+++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPQKRASMVEIMHSPWFQKGFTK---PVSFIEKDEFNNEFSELANPKLLSSSPPFY 310
           +L  +P  R  + +IM + WFQKGF K   P S  E  + ++  S++      S  P  Y
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV--HAAFSVKPMSY 306

Query: 311 NAFEFISSMSSGFDLSSLFESKKKQRSIFTSKCPASAILAKLQSLAVKLNFKATNLKEFK 370
           NAF+ ISS+S GFDLS LFE +++  S FT+K  A  I++K + +A           +  
Sbjct: 307 NAFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVG 366

Query: 371 VRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPALKDIVWS 430
           V+++ K EGRKG L +  E++EV     +VEF KS GDT+EY + C++++RP+LKDIVW 
Sbjct: 367 VKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWK 426

Query: 431 WQGEN 433
           WQG N
Sbjct: 427 WQGNN 427

BLAST of Chy4G080600 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 454.5 bits (1168), Expect = 9.7e-128
Identity = 240/425 (56.47%), Postives = 309/425 (72.71%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKKGLLEQIRREISVMGL 70
           +L G+YE+GRLLG GTFAKVY+ RN+ T +SVA+KV+ K++V K G+++QI+REISVM +
Sbjct: 19  LLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRM 78

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYFQQLISAVDFC 130
           V+HP+IVEL EVMA+KSK+YF ME V GGELF K+++ G+L ED AR YFQQLISAVDFC
Sbjct: 79  VKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAK-GRLREDVARVYFQQLISAVDFC 138

Query: 131 HSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLD  GNLKV+DFGLSA  E L+ DGLLHT CGTPAYVAPEV+ K
Sbjct: 139 HSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILK 198

Query: 191 RGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWFSSEAKEMVSK 250
           +GYDGAKAD+WSCGVILFVLLAG LPF+ DN + +YRKI++ +F+ P W SS+A+ +V+K
Sbjct: 199 KGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTK 258

Query: 251 LLVVDPQKRASMVEIMHSPWFQKGFTK----PV-SFIEKDEFNNEFSELANPKLLSSSPP 310
           LL  +P  R ++ ++M SPWF+K  T+    PV + I   E + +F    + +   +   
Sbjct: 259 LLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKSKEETET--- 318

Query: 311 FYNAFEFISSMSSGFDLSSLFESKKKQRS---IFTSKCPASAILAKLQSLAVKLNFKATN 370
             NAF  I ++S GFDLS LFE KKK+      F +  PAS++++ L+  A   N     
Sbjct: 319 -LNAFHII-ALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVR 378

Query: 371 LKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAVVEFSKSAGDTLEYNKLCEKDVRPALK 428
             E +VR++ K+ GRKGKL V AE++ VAP   VVE  K  GDTLEYN  C   +RPALK
Sbjct: 379 KSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALK 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W1D52.1e-16767.97CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=37... [more]
Q9LEU71.5e-16566.59CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=370... [more]
Q9SEZ72.4e-14761.05CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=37... [more]
Q6ERS42.5e-14458.69CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q9FJ545.2e-13457.18CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A5A7T8B46.8e-24697.08Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CN526.8e-24697.08Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1F2J43.6e-22390.76Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1J0B34.0e-22290.53Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1DJA13.3e-21687.99Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
XP_004137659.10.098.43CBL-interacting serine/threonine-protein kinase 25 [Cucumis sativus][more]
XP_008465153.15.63e-31397.08PREDICTED: CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo... [more]
XP_038905015.13.14e-29693.26CBL-interacting serine/threonine-protein kinase 25-like [Benincasa hispida][more]
KAG6581626.19.89e-28490.76CBL-interacting serine/threonine-protein kinase 25, partial [Cucurbita argyrospe... [more]
XP_022934429.11.99e-28390.76CBL-interacting serine/threonine-protein kinase 25-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G25110.11.5e-16867.97CBL-interacting protein kinase 25 [more]
AT5G10930.11.1e-16666.59CBL-interacting protein kinase 5 [more]
AT2G25090.11.7e-14861.05CBL-interacting protein kinase 16 [more]
AT5G45820.13.7e-13557.18CBL-interacting protein kinase 20 [more]
AT4G30960.19.7e-12856.47SOS3-interacting protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 16..271
e-value: 2.4E-103
score: 359.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 16..271
e-value: 2.4E-75
score: 253.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 16..271
score: 51.692997
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 307..427
e-value: 8.4E-41
score: 140.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 8..306
e-value: 8.5E-102
score: 342.6
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 10..274
e-value: 7.7E-44
score: 147.7
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 15..268
e-value: 6.9E-11
score: 39.5
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 15..271
e-value: 6.5E-38
score: 127.6
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 5..275
e-value: 4.4E-77
score: 258.0
NoneNo IPR availablePANTHERPTHR43895:SF119CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 25-RELATEDcoord: 9..434
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 9..434
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 308..422
e-value: 1.75603E-44
score: 149.26
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 15..270
e-value: 1.59891E-166
score: 466.496
IPR004041NAF domainPFAMPF03822NAFcoord: 304..360
e-value: 8.2E-17
score: 61.1
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 22..54
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 135..147
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 302..327
score: 12.470862
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 12..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G080600.1Chy4G080600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity