Chy4G080430 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G080430
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	4-coumarate:CoA ligase
Location	chrH04: 19794468 .. 19797638 (-)
RNA-Seq Expression	Chy4G080430
Synteny	Chy4G080430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGATTGAAACAGTTGAGAATGACGTCATTTTCCGGTCTAAACTACCGGACATCTACATCCCGAAACATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGGACTTGCCTGATCAACGGCATCACCGGGGAATCCTTCACCTACAACGACGTCGATCTTACCGCACGCAAGGTGGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAATTCGTCTTTGCTTTCATCGGGGCCTCCTACCTCGGTGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGGAAAGCAAGCAAAAGGATCAAAATCAAAACTAATCATAACGCAATCATCGTACTACGAAAAAGTAAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATAATGACCGTCGATTCTCCTCCCGACGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGGTTCCGAGGGTGGATATCGACCCAGACGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCGAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGACATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCGGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATCATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGCGGAGAAATATGGAGTTACCGTTGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTTGAGAAGTATGACCTTTCGTCAATCAAAATTATTAAATCCGGCGGTGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCTAAGGCTGTGCTCGGACAGGTATCATATCAACATAATACAAATTTGTAATTTTAATAACTTTAACAACCCTTTGGTTTTTTTGTTTTTAAAAAATCAATTCTCAAAAAACAAATTTTTTAGTAAAAAAAAGAATATTGAATTTGTTATACTCTCAATTTTTAGTTACTGGTTCGGTTAAATAACAATAAGAAACAAAATAATTTTTTTTTTTAAAAAAATCCTATTGATATTTTTAAAAACCTAATTTTTTGTTTATGTTTTAGAATGAGTTGCAAAAAGGTAGGTAACTACACACAAATATGACTCAATTTTTTAAGTTATATTATACACACAACAATTGCAGTCATCAATAATATCAAGCTAAAATACAAATTTTTAAAGTGAATGAATTTACATCGCAGTTTAATTTTTAATCATTAATGGTATAGATTGAAGTAATTGATATGTATTTATTTTATTAAAAAAAGTTTAAGATTTAATTGGTGGAGATTAGTGGTTATTAAATGTAATTAAGATTTTGTAAATGTGAAGAAAGTCTTTGAGTGGAGACGAAATGACACCGAAGTAAAATCAAAAGTCTGCGTGTAATTCTTTTTATAAAAAAAAAATCGATCAATTTAATTAATTTTTCATTAATTAACATTTATTTATTAATTTATTTTCATTTCCCAATTTGTATTCGAAAGCTTTCGTCCACCTAAACAACACCCCACGAAAAACAAAAAGCAAAACCAAGTTGGTACATTTTTCACCTAAAACCTTTCATCAATAATTTTATATTTTTAAACACACTTTTACACTATTAATACTAATATTTATTTTAAATGAAAAACATTTTTACCAATACTTTTATAAATGATAATAATAACACCTACAATAATAATAAAGATTAGATTACCAAAAAAAAAATTATAGTGTTGATGAGAATGTAATAGAATCTGAAATTTAGTCGTAAATATTTGGAATTAGATTCGCAAATGTACAATAGCTTACGTTTTTTCACAAAATTACTTTAAACTTACGTGGCATAACAATAGCCGTTGGATGATGAAGATTTGAAATAGAGAGAAAATGCGGTTGATTGTAGGGGTACGGAATGACGGAGGCGGGTCCAGTCCTAACAATGGGATTAGCATTTGCAAAGGACCCATTTCCAATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCGGAGATGAAGATCGTCGACACCGAAACCGGCAGTTCCTTGCCTCGTAACACGCCGGGAGAGATCTGCATCAGAGGTGATCAAATCATGAAAGGCTATCTCAACAATCCAGAGGCAACGGCGGCCACTATCGACAAGGATGGGTGGTTGCACACCGGGGATATTGGATTCATCGACGACGATGATGAGATGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCGTAAGAATAACAACCAATTGAATTTGGATTTCGGTTAGTGAAAGATGGTTGGGTGATGATTGTTGGGTTAATTTTGCAGAATGAAAGATGAACAAGCTGGAGAAGTTCCCGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAGTTTATTTCAAAACAGGTATCCTTTTTTTCTCCATAACAAATTTAATTTTTTTTTAAAAAAAATGATCATTATTTGCTTTTTATAACTTTTTCTCAAATTCGATTGACCATTATTATTTTTATTTTTTTAAAATAAAGTTTGAAACCAAATGTAGTGAAAAAAAAGATTCAAAATAAAAATTTATATTATAAGTAACTACAATATTAATATGAAGATAGAGAATTGAACCTCAAATCTCTTAAACATAACCCATTGATTACAATATATGAAATGGAAAAATCAAATGATAGACTCTAAAGTTGATCATCAAATGCAATATTTATGTTAAAAGATTAAATATATATTTGATTTGGTGTGGAATATTGATTAGGAATATAAAATTGAAAAAACCGTGCAGGTGGTTTTCTACAAAAGAATAAACCGGGTGTTTTTCATAGATGCAATTCCCAAATCGCCCTCGGGAAAGATATTGAGGAAGGAGTTGAGGGCAAAGTTAGCTGCTGCTTTTCCAAATTCTAATTAA

mRNA sequence

ATGGGGATTGAAACAGTTGAGAATGACGTCATTTTCCGGTCTAAACTACCGGACATCTACATCCCGAAACATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGGACTTGCCTGATCAACGGCATCACCGGGGAATCCTTCACCTACAACGACGTCGATCTTACCGCACGCAAGGTGGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAATTCGTCTTTGCTTTCATCGGGGCCTCCTACCTCGGTGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGGAAAGCAAGCAAAAGGATCAAAATCAAAACTAATCATAACGCAATCATCGTACTACGAAAAAGTAAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATAATGACCGTCGATTCTCCTCCCGACGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGGTTCCGAGGGTGGATATCGACCCAGACGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCGAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGACATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCGGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATCATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGCGGAGAAATATGGAGTTACCGTTGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTTGAGAAGTATGACCTTTCGTCAATCAAAATTATTAAATCCGGCGGTGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCTAAGGCTGTGCTCGGACAGGGGTACGGAATGACGGAGGCGGGTCCAGTCCTAACAATGGGATTAGCATTTGCAAAGGACCCATTTCCAATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCGGAGATGAAGATCGTCGACACCGAAACCGGCAGTTCCTTGCCTCGTAACACGCCGGGAGAGATCTGCATCAGAGGTGATCAAATCATGAAAGGCTATCTCAACAATCCAGAGGCAACGGCGGCCACTATCGACAAGGATGGGTGGTTGCACACCGGGGATATTGGATTCATCGACGACGATGATGAGATGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCAATGAAAGATGAACAAGCTGGAGAAGTTCCCGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAGTTTATTTCAAAACAGGTGGTTTTCTACAAAAGAATAAACCGGGTGTTTTTCATAGATGCAATTCCCAAATCGCCCTCGGGAAAGATATTGAGGAAGGAGTTGAGGGCAAAGTTAGCTGCTGCTTTTCCAAATTCTAATTAA

Coding sequence (CDS)

ATGGGGATTGAAACAGTTGAGAATGACGTCATTTTCCGGTCTAAACTACCGGACATCTACATCCCGAAACATCTTCCTCTGCATTCCTACTGCCTACAAGAAAACGCCGCCAAAATAGGCCACCGGACTTGCCTGATCAACGGCATCACCGGGGAATCCTTCACCTACAACGACGTCGATCTTACCGCACGCAAGGTGGCCTCCGGATTAAACAAACTCGGCATTACAAAACGCGACGTAATCATGCTCCTCCTCCCAAATTCACCGGAATTCGTCTTTGCTTTCATCGGGGCCTCCTACCTCGGTGCAATCATGACGGCCGCAAACCCTTTCTTCACGGCAGCAGAAATCGGAAAGCAAGCAAAAGGATCAAAATCAAAACTAATCATAACGCAATCATCGTACTACGAAAAAGTAAAGGAAATAACAGAGGAATTGCCAGAGGTTAAAATAATGACCGTCGATTCTCCTCCCGACGGTTGTTTATGGTTCGGTGATCTAATTAAAGCCGACGAAAGAGAGGTTCCGAGGGTGGATATCGACCCAGACGACGTGGTTGCGCTGCCGTATTCGTCGGGAACGACGGGATTGCCGAAGGGAGTGATGCTTACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGGGAAAATCCAAATCTGTATTATGGACATGAGGATGTGATTCTGTGTGTTTTGCCGTTGTTTCATATTTATTCTTTGAATTCGGTTTTGCTGTGTGGATTAAGAGCTGGAAGTACCATTTTGATCATGCCCAAATTTGAGATCGGTTTGCTTTTGCAATTGGCGGAGAAATATGGAGTTACCGTTGCGCCCATCGTGCCGCCCATCGTTCTGGCTATTGCAAAGTCGCCGGAGCTTGAGAAGTATGACCTTTCGTCAATCAAAATTATTAAATCCGGCGGTGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCCAAGTTTCCTAAGGCTGTGCTCGGACAGGGGTACGGAATGACGGAGGCGGGTCCAGTCCTAACAATGGGATTAGCATTTGCAAAGGACCCATTTCCAATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCGGAGATGAAGATCGTCGACACCGAAACCGGCAGTTCCTTGCCTCGTAACACGCCGGGAGAGATCTGCATCAGAGGTGATCAAATCATGAAAGGCTATCTCAACAATCCAGAGGCAACGGCGGCCACTATCGACAAGGATGGGTGGTTGCACACCGGGGATATTGGATTCATCGACGACGATGATGAGATGTTCATTGTGGATCGGTTGAAGGAACTGATTAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGTTGACTCATCCGGTCATCTCTGATGCCGCCGTTGTTCCAATGAAAGATGAACAAGCTGGAGAAGTTCCCGTCGCGTTCGTTGTGAAATTGAAGAATTCTGAAGCCACTGAAGATGAAATTAAACAGTTTATTTCAAAACAGGTGGTTTTCTACAAAAGAATAAACCGGGTGTTTTTCATAGATGCAATTCCCAAATCGCCCTCGGGAAAGATATTGAGGAAGGAGTTGAGGGCAAAGTTAGCTGCTGCTTTTCCAAATTCTAATTAA

Protein sequence

MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPNSN*

Homology

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BLAST of Chy4G080430 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 1.5e-231
Identity = 402/541 (74.31%), Postives = 457/541 (84.47%), Query Frame = 0


Query: 4   ETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTA 63
           +T + D+IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING   + +TY DV+L +
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVELNS 63

Query: 64  RKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKG 123
           RKVA+GL+K GI  +D IM+LLPNSPEFVFAFIGASYLGAI T ANP FT AE+ KQAK 
Sbjct: 64  RKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 123

Query: 124 SKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPD 183
           S +K+I+TQ+ +  KVK+   E  +VKI+ +DS P+GCL F  L +A+E ++P V+I PD
Sbjct: 124 SSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPD 183

Query: 184 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSL 243
           DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY   EDV+LCVLPLFHIYSL
Sbjct: 184 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSL 243

Query: 244 NSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSS 303
           NSVLLCGLR G+ ILIM KF+I   L+L ++Y VT+ P VPPIVLAIAKSP ++ YDLSS
Sbjct: 244 NSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSS 303

Query: 304 IKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACG 363
           ++ + SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAK+PF +K GACG
Sbjct: 304 VRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACG 363

Query: 364 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 423
           TVVRNAEMKIVD +TG+SLPRN  GEICIRGDQIMKGYLN+PEATA TIDK+GWL+TGDI
Sbjct: 364 TVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDI 423

Query: 424 GFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 483
           G+IDDDDE+FIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEVPVA
Sbjct: 424 GYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVA 483

Query: 484 FVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFP 543
           FVV+   S  TEDE+K FISKQV+FYKRI RVFF+DAIPKSPSGKILRK+LRAKLAA  P
Sbjct: 484 FVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLP 542

Query: 544 N 545
           N
Sbjct: 544 N 542

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BLAST of Chy4G080430 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 1.2e-230
Identity = 395/536 (73.69%), Postives = 451/536 (84.14%), Query Frame = 0


Query: 9   DVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTARKVAS 68
           D+IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING   + +TY +V+LT RKVA 
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELTCRKVAV 73

Query: 69  GLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKGSKSKL 128
           GLNKLGI ++D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQAK S +K+
Sbjct: 74  GLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKI 133

Query: 129 IITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPDDVVAL 188
           IITQS +  KVK+   E  +VK++ +DS P+GCL F +L ++DE E+P V I PDDVVAL
Sbjct: 134 IITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDDVVAL 193

Query: 189 PYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVLL 248
           PYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY   EDV++CVLPLFHIYSLNS+LL
Sbjct: 194 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILL 253

Query: 249 CGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIKIIK 308
           CGLR G+ ILIM KF+I   L+L +KY V++ P VPPIVLAIAKSP ++ YDLSS++ + 
Sbjct: 254 CGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVM 313

Query: 309 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVRN 368
           SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAK+PF +K GACGTVVRN
Sbjct: 314 SGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRN 373

Query: 369 AEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDD 428
           AEMKIVD +TG SLPRN PGEICIRGDQIMKGYLN+PEAT  TIDK+GWLHTGDIGFID+
Sbjct: 374 AEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDE 433

Query: 429 DDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKL 488
           DDE+FIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQAGEVPVAFVV+ 
Sbjct: 434 DDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRS 493

Query: 489 KNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAAFPN 545
             S  TEDE+K FISKQV+FYKR+ RVFF++ +PKSPSGKILRK+LRA+LAA  PN
Sbjct: 494 NGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

[Close]

BLAST of Chy4G080430 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 2.3e-229
Identity = 395/544 (72.61%), Postives = 452/544 (83.09%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           M  ET + D+IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING     +TY DV+
Sbjct: 3   METETNQGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           LT+RKVA+GLNKLGI ++D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AK S +KLIITQ+ +  KVK+   +   + ++ +DS P+GC+ F +L +ADE ++P V I
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFD-NNLNVICIDSAPEGCIHFSELTQADEHDIPDVKI 182

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
             DDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY   EDV++CVLPLFHI
Sbjct: 183 QSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 242

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLR G+ ILIM KF+I    +L EKY VT+ P VPPIVLAIAKSP ++ YD
Sbjct: 243 YSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYD 302

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSS++ + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAK+PF +K G
Sbjct: 303 LSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 362

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVD +TG SLPRN PGEICIRGDQIMKGYLN+P AT  TIDK+GWLHT
Sbjct: 363 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHT 422

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG+ID+DDE+FIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEV
Sbjct: 423 GDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 482

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+   S+ TEDE+K F+SKQV+FYKRI RVFF++ +PKSPSGKILRK+LRA+LAA
Sbjct: 483 PVAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAA 542

Query: 541 AFPN 545
             PN
Sbjct: 543 GVPN 544

[Close]

BLAST of Chy4G080430 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 2.3e-229
Identity = 407/546 (74.54%), Postives = 457/546 (83.70%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           + IE  + D+IFRSKLPDIYIPK+LPLHSYC  EN +K   R CLING T E FTY DV+
Sbjct: 5   VAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCF-ENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           L +R+V SGL+KLGI + D IM+LLPNSPEFVFAF+GAS++G+I T ANPFFT+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPD-----GCLWFGDLIKADEREV 180
           AK S +KLIITQ  Y +KVK+   E   VKI+++D+          L F +L  ADE E+
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACE-NGVKIISIDTTTTADDAANILHFSELTGADENEM 184

Query: 181 PRVDIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVL 240
           P+V+I PD VVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY   +DV+LCVL
Sbjct: 185 PKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVL 244

Query: 241 PLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPE 300
           PLFHIYSLNSVLLCGLRAGS ILIM KFEI   L+L +KY VT+ P VPPIVLAIAKS  
Sbjct: 245 PLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTV 304

Query: 301 LEKYDLSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPF 360
           ++ YDLSS++ + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAK+PF
Sbjct: 305 VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 364

Query: 361 PMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKD 420
            +K GACGTVVRNAEMKIVD ETGSSLPRN PGEICIRGDQIMKGYLN+PEATA TIDK+
Sbjct: 365 DIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKE 424

Query: 421 GWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE 480
           GWLHTGDIG+IDDDDE+FIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE
Sbjct: 425 GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDE 484

Query: 481 QAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELR 540
            AGEVPVAFVVK      TEDEIKQFISKQV+FYKRINRVFF++AIPK+PSGKILRK+LR
Sbjct: 485 AAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLR 544

Query: 541 AKLAAA 542
           A+LAAA
Sbjct: 545 ARLAAA 548

[Close]

BLAST of Chy4G080430 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 6.8e-229
Identity = 396/534 (74.16%), Postives = 455/534 (85.21%), Query Frame = 0


Query: 7   ENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTARKV 66
           + D+IFRSKLPDIYIPKHLPLH+YC  EN +K+G ++CLING TGE+FTY+ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  ASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKGSKS 126
           ASGLNKLGI + D IMLLLPNSPE+ FAF+GASY GAI T ANPFFT+AE+ KQ K S +
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127

Query: 127 KLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDIDPDDVV 186
           KLIITQ+ Y +KVK+   E   ++I+ +D  P  CL F  L++ADE E+P V ID DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY   EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIKI 306
           L CGLRAG TILIM KF+I   L+L +KY VT+ P VPPIVLAIAKSP ++KYDLSS++ 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVV 366
           + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAK+P+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 426
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           DDDDE+FIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 541
           +      TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRAK+A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIAS 539

[Close]

BLAST of Chy4G080430 vs. ExPASy TrEMBL
Match: A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 6.1e-305
Identity = 527/546 (96.52%), Postives = 542/546 (99.27%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           MGIETVENDVIFRSKLPDIYIP HLPLHSYCLQENAAKIGHRTCLING+TGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAF+GAS+LGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGSK+KLIITQSSYYEK+KEITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+I
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVVKLKNSEATEDEIKQFISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
           AFPNSN
Sbjct: 541 AFPNSN 546

[Close]

BLAST of Chy4G080430 vs. ExPASy TrEMBL
Match: A0A1S3CJP5 (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103501216 PE=4 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 3.2e-282
Identity = 492/519 (94.80%), Postives = 509/519 (98.07%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           MGIETVENDVIFRSKLPDIYIP HLPLHSYCLQENAAKIGHRTCLING+TGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAF+GAS+LGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGSK+KLIITQSSYYEK+KEITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+I
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQV-VFY--KRINRVF 517
           PVAFVVKLKNSEATEDEIKQFISKQV VF+    +N +F
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVSVFFYLTNLNSIF 519

[Close]

BLAST of Chy4G080430 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 9.4e-282
Identity = 484/546 (88.64%), Postives = 515/546 (94.32%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           M +E ++ND IFRSKLPDIYIP HLPLHSYCL EN AKIGHRTCLIN +TGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           L ARKVASGLNKLGI + DVIML+L NSPEFVFAF+GASY GAIMTAANPFFTAAEI KQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGS +KLI+TQSSYYEKVKEITEELP+VKIMTVDSPPDGCL F DLI+ADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY +EDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQL +K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDE+FIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Chy4G080430 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.3e-279
Identity = 481/546 (88.10%), Postives = 512/546 (93.77%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           M +E ++ND IFRSKLPDIYIP HLPLHSYCL EN AKIGHRTCLIN +T ESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           L ARKVASGLNKLGI + DVI+LLL NSPEFVFAF+GASY GAIMTAANPFFTAAEI KQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGS +KLI+TQSSYYEKVKEITEELP+VKIMTVDSPPDGCL F DLI+ADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY +EDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQL +K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDE+FIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 AFPNSN 547
            FP SN
Sbjct: 541 GFPISN 546

[Close]

BLAST of Chy4G080430 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.0e-276
Identity = 483/546 (88.46%), Postives = 510/546 (93.41%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           M  ETV +D+IFRSKLPDIYIPKHLPLHSYCLQE  A+IGHRTCLIN +TGESFTY+DVD
Sbjct: 1   MANETV-HDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           L ARK ASGL KLGI K DVIMLLLPNSPEFVFAF+GASYLGAIMTAANPFFTAAEI KQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGSK+KL ITQSSYYEKVKEITE+LP+ KIMTVDSPP GCL F DLI+  + E+P V+I
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYY ++DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQL EKY V++APIVPPIVLAIAKSP+LEKYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAE+KIVDTE G SLPRNTPGEICI+GDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDDDDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVVKLKNS+ TEDEIKQFISKQVVFYKRINRVFFI AIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
            FP  N
Sbjct: 541 DFPIPN 545

[Close]

BLAST of Chy4G080430 vs. NCBI nr
Match: XP_004137646.3 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KAE8650952.1 hypothetical protein Csa_000821 [Cucumis sativus])

HSP 1 Score: 1072 bits (2772), Expect = 0.0
Identity = 538/546 (98.53%), Postives = 544/546 (99.63%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLING+TGESFTYNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           LT RKVASGLNKLGITKRDVIMLLLPNSPEFVFAF+GASYLGAIMTAANPFFTAAEIGKQ
Sbjct: 61  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI
Sbjct: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           DP+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI
Sbjct: 181 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI+IIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG
Sbjct: 301 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVVKLKNSEA+EDEIKQFISKQVVFYKRINRVFFI+AIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVKLKNSEASEDEIKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 546
           AFP SN
Sbjct: 541 AFPTSN 546

[Close]

BLAST of Chy4G080430 vs. NCBI nr
Match: KAA0034948.1 (4-coumarate--CoA ligase 1 [Cucumis melo var. makuwa] >TYK21303.1 4-coumarate--CoA ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 527/546 (96.52%), Postives = 542/546 (99.27%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           MGIETVENDVIFRSKLPDIYIP HLPLHSYCLQENAAKIGHRTCLING+TGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAF+GAS+LGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGSK+KLIITQSSYYEK+KEITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+I
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVVKLKNSEATEDEIKQFISKQVVFYK+INRVFFIDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 546
           AFPNSN
Sbjct: 541 AFPNSN 546

[Close]

BLAST of Chy4G080430 vs. NCBI nr
Match: XP_038904387.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 511/546 (93.59%), Postives = 533/546 (97.62%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           MGIETV+NDVIFRSKLPDIYIPKHLPLHSYCL ENAAKIG RTCLING+TGESFTYNDVD
Sbjct: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           L+ARKVA+GLNKLGITK DVIMLLLPNSPEFVF F+GASYLGAIMTAANPFFTA EI KQ
Sbjct: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGSK+KLI+T SSYYEKVKEITEELP+VKIMTVDSPPDGCLWFGDLIKADER++P V+I
Sbjct: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYY +EDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIGLLLQL EKY V++APIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDD+DE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVVKLKNSE TEDEIKQF+SKQVVFYKRINRVFFIDAIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540

Query: 541 AFPNSN 546
           AFPNSN
Sbjct: 541 AFPNSN 546

[Close]

BLAST of Chy4G080430 vs. NCBI nr
Match: XP_008462955.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 492/519 (94.80%), Postives = 509/519 (98.07%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           MGIETVENDVIFRSKLPDIYIP HLPLHSYCLQENAAKIGHRTCLING+TGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAF+GAS+LGAIMTAANPFFTAAEI KQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGSK+KLIITQSSYYEK+KEITEELPEVKIMTVDSP DGCLWFGDLIKADERE+PRV+I
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYG+EDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGSTILIMPKFEIGLLLQL EKYGVTVAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID+DDE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQV-VFY--KRINRVF 516
           PVAFVVKLKNSEATEDEIKQFISKQV VF+    +N +F
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVSVFFYLTNLNSIF 519

[Close]

BLAST of Chy4G080430 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 484/546 (88.64%), Postives = 515/546 (94.32%), Query Frame = 0


Query: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVD 60
           M +E ++ND IFRSKLPDIYIP HLPLHSYCL EN AKIGHRTCLIN +TGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 120
           L ARKVASGLNKLGI + DVIML+L NSPEFVFAF+GASY GAIMTAANPFFTAAEI KQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180
           AKGS +KLI+TQSSYYEKVKEITEELP+VKIMTVDSPPDGCL F DLI+ADERE+P V+I
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYY +EDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIG LLQL +K+ V++APIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360
           LSSI++IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDDDE+FIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSE TEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 546
            FPNSN
Sbjct: 541 GFPNSN 546

[Close]

BLAST of Chy4G080430 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 768.1 bits (1982), Expect = 4.8e-222
Identity = 384/544 (70.59%), Postives = 455/544 (83.64%), Query Frame = 0


Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTARKVA 67
           NDVIFRS+LPDIYIP HLPLH Y + EN ++   + CLING TGE +TY DV +T+RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 68  SGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKGSKSK 127
           +GL+ LG+ + DV+M+LLPNSPE V  F+ AS++GAI T+ANPFFT AEI KQAK S +K
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 128 LIITQSSYYEKVKEITEELPEVKIMTVDSP--PDGCLWFGDLIKADEREVPRVD-----I 187
           LI+TQS Y +K+K +  +   V I+T DS   P+ CL F +L +++E   PRVD     I
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEE---PRVDSIPEKI 198

Query: 188 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 247
            P+DVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHI
Sbjct: 199 SPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHI 258

Query: 248 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 307
           Y+LNS++LC LR G+TILIMPKFEI LLL+  ++  VTVA +VPPIVLAIAKSPE EKYD
Sbjct: 259 YALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYD 318

Query: 308 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 367
           LSS++++KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAK+PFP+K G
Sbjct: 319 LSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 378

Query: 368 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 427
           ACGTVVRNAEMKI+D +TG SLPRN PGEICIRG+QIMKGYLN+P ATA+TIDKDGWLHT
Sbjct: 379 ACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHT 438

Query: 428 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 487
           GD+GFIDDDDE+FIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEV
Sbjct: 439 GDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEV 498

Query: 488 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 545
           PVAFVV+ K+S  +EDEIKQF+SKQVVFYKRIN+VFF D+IPK+PSGKILRK+LRA+LA 
Sbjct: 499 PVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLAN 556

[Close]

BLAST of Chy4G080430 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 755.4 bits (1949), Expect = 3.3e-218
Identity = 379/539 (70.32%), Postives = 443/539 (82.19%), Query Frame = 0


Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTARKVA 67
           +DVIFRSKLPDIYIP HL LH Y  Q N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKGSKSK 127
           +  +KLG+ + DV+MLLLPN PEFV +F+ AS+ GA  TAANPFFT AEI KQAK S +K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIITQSSYYEKVKEITEELPEVKIMTVDSP----PDGCLWFGDLIKAD---EREVPRVDI 187
           LIIT++ Y +K+K +  +   V +   D+     P+GCL F +L ++       +  V+I
Sbjct: 141 LIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 200

Query: 188 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 247
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHI
Sbjct: 201 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 260

Query: 248 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 307
           Y+LNS++LCGLR G+ ILIMPKFEI LLL+L ++  VTVAP+VPPIVLAIAKS E EKYD
Sbjct: 261 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 320

Query: 308 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 367
           LSSI+++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAK+PFP+K G
Sbjct: 321 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 380

Query: 368 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 427
           ACGTVVRNAEMKIVD +TG SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLHT
Sbjct: 381 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 440

Query: 428 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 487
           GDIG IDDDDE+FIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 441 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 500

Query: 488 PVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           PVAFVVK K+SE +ED++KQF+SKQVVFYKRIN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 501 PVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

[Close]

BLAST of Chy4G080430 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 699.9 bits (1805), Expect = 1.6e-201
Identity = 352/506 (69.57%), Postives = 411/506 (81.23%), Query Frame = 0


Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTARKVA 67
           +DVIFRSKLPDIYIP HL LH Y  Q N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  SGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKGSKSK 127
           +  +KLG+ + DV+MLLLPN PEFV +F+ AS+ GA  TAANPFFT AEI KQAK S +K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LIITQSSYYEKVKEITEELPEVKIMTVDSP----PDGCLWFGDLIKAD---EREVPRVDI 187
           LIIT++ Y +K+K +  +   V +   D+     P+GCL F +L ++       +  V+I
Sbjct: 141 LIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 200

Query: 188 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 247
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHI
Sbjct: 201 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 260

Query: 248 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 307
           Y+LNS++LCGLR G+ ILIMPKFEI LLL+L ++  VTVAP+VPPIVLAIAKS E EKYD
Sbjct: 261 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 320

Query: 308 LSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 367
           LSSI+++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAK+PFP+K G
Sbjct: 321 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 380

Query: 368 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 427
           ACGTVVRNAEMKIVD +TG SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLHT
Sbjct: 381 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 440

Query: 428 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 487
           GDIG IDDDDE+FIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 441 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 500

Query: 488 PVAFVVKLKNSEATEDEIKQFISKQV 507
           PVAFVVK K+SE +ED++KQF+SKQV
Sbjct: 501 PVAFVVKSKDSELSEDDVKQFVSKQV 525

[Close]

BLAST of Chy4G080430 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 684.5 bits (1765), Expect = 7.0e-197
Identity = 340/531 (64.03%), Postives = 425/531 (80.04%), Query Frame = 0


Query: 11  IFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGITGESFTYNDVDLTARKVASGL 70
           IFRSKLPDI IP HLPLH+YC  E  + +  + CLI G TG+S+TY +  L  R+VASGL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 71  NKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQAKGSKSKLII 130
            KLGI K DVIM+LL NS EFVF+F+GAS +GA+ T ANPF+T+ E+ KQ K S +KLII
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 131 TQSSYYEKVKEITEELPEVKIMTVDSP-PDGCLWFGDLIKADERE--VPRVDIDPDDVVA 190
           T S Y +K+K + E L    ++T D P P+ CL F  LI  DE       VDI  DD  A
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHIYSLNSVL 250
           LP+SSGTTGLPKGV+LTHKSL+TSVAQQVDG+NPNLY    DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 LCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYDLSSIKII 310
           L  LR+G+T+L+M KFEIG LL L +++ VT+A +VPP+V+A+AK+P +  YDLSS++ +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 KSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPGACGTVVR 370
            SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAK+P P K G+CGTVVR
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 371 NAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID 430
           NAE+K+V  ET  SL  N PGEICIRG QIMK YLN+PEAT+ATID++GWLHTGDIG++D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 431 DDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVK 490
           +DDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I+DAAVVP  DE AGEVPVAFVV+
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 491 LKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 539
              ++ TE+++K++++KQVVFYKR+++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

[Close]

BLAST of Chy4G080430 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 669.1 bits (1725), Expect = 3.1e-192
Identity = 330/545 (60.55%), Postives = 415/545 (76.15%), Query Frame = 0


Query: 4   ETVENDVIFRSKLPDIYIPKHLPLHSYCLQE---NAAKIGHRTCLINGITGESFTYNDVD 63
           E   +D IFRSKLPDI+IP HLPL  Y  Q    +       TC+I+G TG   TY DV 
Sbjct: 21  EEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQ 80

Query: 64  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFIGASYLGAIMTAANPFFTAAEIGKQ 123
              R++A+G+++LGI   DV+MLLLPNSPEF  +F+  +YLGA+ T ANPF+T  EI KQ
Sbjct: 81  TNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQ 140

Query: 124 AKGSKSKLIITQSSYYEKVKEITEELPEVKIM-------TVDSPPDGCLWFGDLIKADER 183
           AK S +K+IIT+    +K+  +  +   +  +        V S  DGC+ F +L +ADE 
Sbjct: 141 AKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADET 200

Query: 184 EVPRVDIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILC 243
           E+ +  I P+D VA+PYSSGTTGLPKGVM+THK LVTS+AQ+VDGENPNL +   DVILC
Sbjct: 201 ELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILC 260

Query: 244 VLPLFHIYSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKS 303
            LP+FHIY+L++++L  +R G+ +LI+P+FE+ L+++L ++Y VTV P+ PP+VLA  KS
Sbjct: 261 FLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKS 320

Query: 304 PELEKYDLSSIKIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKD 363
           PE E+YDLSS++I+ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK+
Sbjct: 321 PETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKN 380

Query: 364 PFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATID 423
           PF  K GACGTV+RNAEMK+VDTETG SLPRN  GEIC+RG Q+MKGYLN+PEATA TID
Sbjct: 381 PFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTID 440

Query: 424 KDGWLHTGDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMK 483
           KDGWLHTGDIGF+DDDDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MK
Sbjct: 441 KDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMK 500

Query: 484 DEQAGEVPVAFVVKLKNSEATEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKE 539
           DE A EVPVAFV + + S+ TED++K +++KQVV YKRI  VFFI+ IPK+ SGKILRK+
Sbjct: 501 DEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKD 560

The following BLAST results are available for this feature:

BLAST of Chy4G080430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24146	1.5e-231	74.31	4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1	[more]
O24145	1.2e-230	73.69	4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1	[more]
I3PB37	2.3e-229	72.61	4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1	[more]
O24540	2.3e-229	74.54	4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1	[more]
P14913	6.8e-229	74.16	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1	[more]

BLAST of Chy4G080430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SWF8	6.1e-305	96.52	4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CJP5	3.2e-282	94.80	4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103501216 PE=4 SV=1	[more]
A0A6J1F599	9.4e-282	88.64	4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=...	[more]
A0A6J1J912	3.3e-279	88.10	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 ...	[more]
A0A6J1KCG9	2.0e-276	88.46	4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 ...	[more]

BLAST of Chy4G080430 vs. NCBI nr


Match Name	Identity	Description
XP_004137646.3	98.53	4-coumarate--CoA ligase 1 [Cucumis sativus] >KAE8650952.1 hypothetical protein C...	[more]
KAA0034948.1	96.52	4-coumarate--CoA ligase 1 [Cucumis melo var. makuwa] >TYK21303.1 4-coumarate--Co...	[more]
XP_038904387.1	93.59	4-coumarate--CoA ligase 1-like [Benincasa hispida]	[more]
XP_008462955.1	94.80	PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo]	[more]
XP_022935389.1	88.64	4-coumarate--CoA ligase 1-like [Cucurbita moschata]	[more]

BLAST of Chy4G080430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21240.1	4.8e-222	70.59	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	3.3e-218	70.32	4-coumarate:CoA ligase 1	[more]
AT1G51680.3	1.6e-201	69.57	4-coumarate:CoA ligase 1	[more]
AT1G65060.1	7.0e-197	64.03	4-coumarate:CoA ligase 3	[more]
AT3G21230.1	3.1e-192	60.55	4-coumarate:CoA ligase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 32..444 e-value: 1.3E-110 score: 369.9
None	No IPR available	GENE3D	3.30.300.30		coord: 439..541 e-value: 1.5E-32 score: 114.3
None	No IPR available	PANTHER	PTHR24096:SF382	4-COUMARATE--COA LIGASE 2-LIKE	coord: 7..540
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 7..540
None	No IPR available	CDD	cd05904	4CL	coord: 21..534 e-value: 0.0 score: 766.012
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 20..540
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 453..528 e-value: 1.4E-16 score: 61.2
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 5..438 e-value: 5.9E-169 score: 564.2
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 188..199

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G080430.1	Chy4G080430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity