Chy4G080270 (gene) Cucumber (hystrix) v1

BLAST of Chy4G080270 vs. ExPASy Swiss-Prot
Match: O64876 (Protein N-terminal asparagine amidohydrolase OS=Arabidopsis thaliana OX=3702 GN=NTAN1 PE=2 SV=2)

HSP 1 Score: 392.9 bits (1008), Expect = 3.8e-108
Identity = 190/350 (54.29%), Postives = 255/350 (72.86%), Query Frame = 0

Query: 1   MIFVDGAPFTLQS-----SSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGV 60
           MI+V G  F  +S     SSS++G+ +L  +M  P +  A++ FK + E   +  +ES  
Sbjct: 1   MIYVGGVQFLDESSSFSLSSSSQGSSLLVDVMSHPVITLASDSFKNLEEKNVSF-DESDS 60

Query: 61  ERPTMSKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQ 120
           E  T  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+ IRNRK+GMTSVAHMD P+I+ 
Sbjct: 61  ESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNRKSGMTSVAHMDSPEIVD 120

Query: 121 IALSQMLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIG 180
           + +SQML LV+    DAELDVH+VGG+EDV +K  + +   GD  K EGYS PLC K++ 
Sbjct: 121 LGISQMLLLVLQDDVDAELDVHMVGGYEDVDIKNADGV---GDYAKPEGYSFPLCCKLVE 180

Query: 181 SLWTRPEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEI 240
           +L  R E FH+QTL IL HNT+ DS+ N+ PIFNG  V TS G++ PASF+ TSRCPDEI
Sbjct: 181 TLQKRRENFHIQTLFILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNRTSRCPDEI 240

Query: 241 VRRIRLSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSC 300
           VRRIR+SSS+ED SW+G+LL+TY+T+TD+F I PCRWT         L +L+D EIL +C
Sbjct: 241 VRRIRVSSSFEDSSWKGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLTDEEILTNC 300

Query: 301 STSPSVEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWER 346
           STSPS EGPDFV + RR W YL+++P+W +TFP+++PR+F R  +G W++
Sbjct: 301 STSPSAEGPDFVNSLRRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346

BLAST of Chy4G080270 vs. ExPASy Swiss-Prot
Match: Q64311 (Protein N-terminal asparagine amidohydrolase OS=Mus musculus OX=10090 GN=Ntan1 PE=1 SV=3)

HSP 1 Score: 133.3 bits (334), Expect = 5.4e-30
Identity = 93/288 (32.29%), Postives = 146/288 (50.69%), Query Frame = 0

Query: 64  VYVFQKEYATVDP--ALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQM 123
           +YV Q+E A   P    +  +G+D+ATTC  + +R+  NG T + H D  D  +  +  +
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGSD-TKAEVPLI 106

Query: 124 LSLVVDPTADAE---LDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLW 183
           +S +   +  AE   L+VHLVGGF D             DR+     S  L ++++    
Sbjct: 107 MSSIKSFSEHAECGRLEVHLVGGFSD-------------DRQ----LSQKLTHQLLSEFD 166

Query: 184 TRPEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRR 243
            + +  HL TLC+ + N R ++E N +PI  G AV      ++ ASF    R P+E +R 
Sbjct: 167 KQDDDIHLVTLCVTELNDREENE-NHFPIIYGIAVNIKTAEIYRASFQ--DRGPEEQLRA 226

Query: 244 IRLSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTS 303
            R  +        G ++  Y+ +T+Q RI PC WTP+ Q +   LQ+  D +IL+S STS
Sbjct: 227 ARALAG-------GPMISIYDAKTEQLRIGPCSWTPFPQ-VDFWLQQ-DDKQILESLSTS 286

Query: 304 PSVEGPDFVENARRQWAYLVEHPDWRE-TFPKKKPRIFRRAANGKWER 346
           P  E P FVE+ R    +L + P      FP  K  ++++  +G WE+
Sbjct: 287 PLAEPPHFVEHIRSTLMFLKKFPSPENILFPGNKALLYKKNKDGLWEK 304

BLAST of Chy4G080270 vs. ExPASy Swiss-Prot
Match: Q28955 (Protein N-terminal asparagine amidohydrolase OS=Sus scrofa OX=9823 GN=NTAN1 PE=1 SV=4)

HSP 1 Score: 131.7 bits (330), Expect = 1.6e-29
Identity = 92/287 (32.06%), Postives = 145/287 (50.52%), Query Frame = 0

Query: 64  VYVFQKEYATVDP--ALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDI---IQIAL 123
           +YV Q+E A   P    V  +G+D+ATTC  + +R+  NG T + H D  D    + + +
Sbjct: 47  LYVQQRELAVTSPKDGSVCILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVSLIM 106

Query: 124 SQMLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLW 183
           S + S   D T    L+VHLVGGF D             DR+     S  L ++++    
Sbjct: 107 SSIKSF-SDHTQRGRLEVHLVGGFSD-------------DRQ----LSQKLTHQLLSEFD 166

Query: 184 TRPEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRR 243
            + +  HL TLC+ + N R ++E N +PI  G AV      ++ ASF    R P+E +R 
Sbjct: 167 RQEDDIHLVTLCVTELNDREENE-NHFPIIYGIAVNVKTAEIYRASF--PDRGPEEELRA 226

Query: 244 IRLSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTS 303
            R+ +        G ++  Y+ +T+Q RI P  W P+  H+   LQ+  D +IL++ STS
Sbjct: 227 ARVLTG-------GPMISIYDAKTEQLRIGPYSWMPF-PHVDFWLQQ-DDKQILENLSTS 286

Query: 304 PSVEGPDFVENARRQWAYLVEHPDWRET-FPKKKPRIFRRAANGKWE 345
           P  E P FVE+ R    +L ++P    T FP  K  ++++  +G W+
Sbjct: 287 PLAEPPHFVEHIRSTLMFLKKYPSPTNTLFPGNKALLYKKNEDGLWK 303

BLAST of Chy4G080270 vs. ExPASy Swiss-Prot
Match: Q96AB6 (Protein N-terminal asparagine amidohydrolase OS=Homo sapiens OX=9606 GN=NTAN1 PE=1 SV=3)

HSP 1 Score: 129.4 bits (324), Expect = 7.8e-29
Identity = 89/288 (30.90%), Postives = 143/288 (49.65%), Query Frame = 0

Query: 64  VYVFQKEYATVDP--ALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDI---IQIAL 123
           +YV Q+E A   P    +  +G+D+ATTC  + +R+  NG T + H D  D    + + +
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVPLIM 106

Query: 124 SQMLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLW 183
           + + S   D      L+VHLVGGF D             DR+     S  L ++++    
Sbjct: 107 NSIKSF-SDHAQCGRLEVHLVGGFSD-------------DRQ----LSQKLTHQLLSEFD 166

Query: 184 TRPEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRR 243
            + +  HL TLC+ + N R ++E N +P+  G AV      ++ ASF    R P+E +R 
Sbjct: 167 RQEDDIHLVTLCVTELNDREENE-NHFPVIYGIAVNIKTAEIYRASFQ--DRGPEEQLRA 226

Query: 244 IRLSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTS 303
            R  +        G ++  Y+ +T+Q RI P  WTP+  H+   L +  D +IL++ STS
Sbjct: 227 ARTLAG-------GPMISIYDAETEQLRIGPYSWTPF-PHVDFWLHQ-DDKQILENLSTS 286

Query: 304 PSVEGPDFVENARRQWAYLVEHPDWRET-FPKKKPRIFRRAANGKWER 346
           P  E P FVE+ R    +L +HP    T F   K  ++++  +G WE+
Sbjct: 287 PLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEK 304

BLAST of Chy4G080270 vs. ExPASy TrEMBL
Match: A0A0A0L9W7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G702610 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.4e-201
Identity = 344/347 (99.14%), Postives = 345/347 (99.42%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKG PEIR TVSEESGVERPTM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRVTVSEESGVERPTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNG+TSVAHMDFPDIIQIALSQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGITSVAHMDFPDIIQIALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR
Sbjct: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERSN 348
           VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERSN
Sbjct: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERSN 347

BLAST of Chy4G080270 vs. ExPASy TrEMBL
Match: A0A1S3CFG9 (protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500294 PE=4 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 6.6e-196
Identity = 335/346 (96.82%), Postives = 338/346 (97.69%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKG PEIRFTVSEESGVER TM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRFTVSEESGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDII IALSQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIINIALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITR GDRKKMEGYSLPLCNKII SLWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PEKFHLQTLCIL HNTRRDS GNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDE+VRRIR
Sbjct: 181 PEKFHLQTLCILLHNTRRDSHGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEVVRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDPSWEGRLLETYETQTDQF+IEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFKIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERS 347
           VEGPDFVEN+RRQWAYLVEHPDWRETFPKKK RIFRRAANGKWERS
Sbjct: 301 VEGPDFVENSRRQWAYLVEHPDWRETFPKKKARIFRRAANGKWERS 346

BLAST of Chy4G080270 vs. ExPASy TrEMBL
Match: A0A5A7U4D2 (Protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00060 PE=4 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.3e-185
Identity = 317/329 (96.35%), Postives = 321/329 (97.57%), Query Frame = 0

Query: 18  KGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTMSKWVYVFQKEYATVDPA 77
           +GADVLYALMECPYLVDATNLFKG PEIRFTVSEESGVER TMSKWVYVFQKEYATVDPA
Sbjct: 7   QGADVLYALMECPYLVDATNLFKGTPEIRFTVSEESGVERSTMSKWVYVFQKEYATVDPA 66

Query: 78  LVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQMLSLVVDPTADAELDVH 137
           LVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDII IALSQMLSLVVDPTADAELDVH
Sbjct: 67  LVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIINIALSQMLSLVVDPTADAELDVH 126

Query: 138 LVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTRPEKFHLQTLCILQHNTR 197
           LVGGFEDVLLKENNNITR GDRKKMEGYSLPLCNKII SLWTRPEKFHLQTLCIL HNTR
Sbjct: 127 LVGGFEDVLLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTR 186

Query: 198 RDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIRLSSSYEDPSWEGRLLET 257
           RDS GNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDE+VRRIRLSSSYEDPSWEGRLLET
Sbjct: 187 RDSHGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWEGRLLET 246

Query: 258 YETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPSVEGPDFVENARRQWAYL 317
           YETQTDQF+IEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPSVEGPDFVEN+RRQWAYL
Sbjct: 247 YETQTDQFKIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPSVEGPDFVENSRRQWAYL 306

Query: 318 VEHPDWRETFPKKKPRIFRRAANGKWERS 347
           VEHPDWRETFPKKK RIFRRAANGKWERS
Sbjct: 307 VEHPDWRETFPKKKARIFRRAANGKWERS 335

BLAST of Chy4G080270 vs. ExPASy TrEMBL
Match: A0A6J1FB23 (protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442418 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.0e-173
Identity = 291/346 (84.10%), Postives = 323/346 (93.35%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDG PFT++SSSSNKGADVLYALMECP+LVDAT  FK IPE+RFT SE+ GVER TM
Sbjct: 1   MIFVDGVPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
            KWVY+FQKEYATVDPALVDFVGTDEATTCVG+AIRNRKNGMTSVAH+DFP+II+ ALSQ
Sbjct: 61  CKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDP++DAELDVHLVGGFEDVLLKE+N+IT   DRKK EG+S PLCNKII  LWTR
Sbjct: 121 MLSLVVDPSSDAELDVHLVGGFEDVLLKESNDITELEDRKKTEGHSFPLCNKIIERLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PE FHLQTLC+L HNTRRDSEGN+YPIF+GF+VKTSDGSVFPASFDSTSRCPDE++RRIR
Sbjct: 181 PETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDPS +GRLLETYET TDQF+IEPC W+PWK++MALSLQ+LSD+EILQSCSTSPS
Sbjct: 241 LSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPS 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERS 347
            E PDFVEN+RRQWAYLVEHPDWRE+FP+KKPRIFRRAA+GKWERS
Sbjct: 301 AEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERS 346

BLAST of Chy4G080270 vs. ExPASy TrEMBL
Match: A0A6J1DIG8 (protein N-terminal asparagine amidohydrolase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020342 PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.0e-172
Identity = 296/345 (85.80%), Postives = 315/345 (91.30%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDG PF ++SSSSNKGADVL ALMECP+LV ATN FKGI E+RFTVS ESGVER TM
Sbjct: 1   MIFVDGKPFNIESSSSNKGADVLNALMECPFLVSATNSFKGISEMRFTVSAESGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
           SKWVYVFQKEYATV+PA+VDFVGTDEATTCVGIAIRNRKNGM SVAHMDFPDII  ALSQ
Sbjct: 61  SKWVYVFQKEYATVNPAIVDFVGTDEATTCVGIAIRNRKNGMISVAHMDFPDIIMKALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDP ++AELDVHLVGGFEDV+LKENN+IT  G RKKMEGYS PLC KII SLWTR
Sbjct: 121 MLSLVVDPASNAELDVHLVGGFEDVVLKENNHITGSGSRKKMEGYSFPLCYKIIKSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PE FHLQTLCILQHNTRRDSEGN+YPIFNG AVKTSDGSVFPASFDSTSRCPDE+VRRIR
Sbjct: 181 PENFHLQTLCILQHNTRRDSEGNAYPIFNGLAVKTSDGSVFPASFDSTSRCPDEVVRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDP+  GRLLE YETQTDQF+IEP RW+ WKQHMALSLQ LSDSEILQ+CSTSP 
Sbjct: 241 LSSSYEDPNSMGRLLEAYETQTDQFKIEPFRWSQWKQHMALSLQHLSDSEILQTCSTSPY 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWER 346
            EGPDFVEN RRQWAY+VEHPDWRETFPKKKPRIF RAA+G+WER
Sbjct: 301 AEGPDFVENERRQWAYIVEHPDWRETFPKKKPRIFTRAADGRWER 345

BLAST of Chy4G080270 vs. NCBI nr
Match: XP_004149611.1 (protein N-terminal asparagine amidohydrolase [Cucumis sativus] >KGN58623.1 hypothetical protein Csa_001991 [Cucumis sativus])

HSP 1 Score: 708 bits (1828), Expect = 1.28e-257
Identity = 344/347 (99.14%), Postives = 345/347 (99.42%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKG PEIR TVSEESGVERPTM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRVTVSEESGVERPTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNG+TSVAHMDFPDIIQIALSQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGITSVAHMDFPDIIQIALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR
Sbjct: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERSN 347
           VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERSN
Sbjct: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERSN 347

BLAST of Chy4G080270 vs. NCBI nr
Match: XP_008461769.1 (PREDICTED: protein N-terminal asparagine amidohydrolase isoform X1 [Cucumis melo])

HSP 1 Score: 689 bits (1779), Expect = 3.91e-250
Identity = 335/346 (96.82%), Postives = 338/346 (97.69%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKG PEIRFTVSEESGVER TM
Sbjct: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGTPEIRFTVSEESGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
           SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDII IALSQ
Sbjct: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIINIALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITR GDRKKMEGYSLPLCNKII SLWTR
Sbjct: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PEKFHLQTLCIL HNTRRDS GNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDE+VRRIR
Sbjct: 181 PEKFHLQTLCILLHNTRRDSHGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEVVRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDPSWEGRLLETYETQTDQF+IEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFKIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERS 346
           VEGPDFVEN+RRQWAYLVEHPDWRETFPKKK RIFRRAANGKWERS
Sbjct: 301 VEGPDFVENSRRQWAYLVEHPDWRETFPKKKARIFRRAANGKWERS 346

BLAST of Chy4G080270 vs. NCBI nr
Match: KAA0048299.1 (protein N-terminal asparagine amidohydrolase isoform X1 [Cucumis melo var. makuwa] >TYK10644.1 protein N-terminal asparagine amidohydrolase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 655 bits (1691), Expect = 6.71e-237
Identity = 317/329 (96.35%), Postives = 321/329 (97.57%), Query Frame = 0

Query: 18  KGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTMSKWVYVFQKEYATVDPA 77
           +GADVLYALMECPYLVDATNLFKG PEIRFTVSEESGVER TMSKWVYVFQKEYATVDPA
Sbjct: 7   QGADVLYALMECPYLVDATNLFKGTPEIRFTVSEESGVERSTMSKWVYVFQKEYATVDPA 66

Query: 78  LVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQMLSLVVDPTADAELDVH 137
           LVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDII IALSQMLSLVVDPTADAELDVH
Sbjct: 67  LVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIINIALSQMLSLVVDPTADAELDVH 126

Query: 138 LVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTRPEKFHLQTLCILQHNTR 197
           LVGGFEDVLLKENNNITR GDRKKMEGYSLPLCNKII SLWTRPEKFHLQTLCIL HNTR
Sbjct: 127 LVGGFEDVLLKENNNITRTGDRKKMEGYSLPLCNKIIRSLWTRPEKFHLQTLCILLHNTR 186

Query: 198 RDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIRLSSSYEDPSWEGRLLET 257
           RDS GNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDE+VRRIRLSSSYEDPSWEGRLLET
Sbjct: 187 RDSHGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEVVRRIRLSSSYEDPSWEGRLLET 246

Query: 258 YETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPSVEGPDFVENARRQWAYL 317
           YETQTDQF+IEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPSVEGPDFVEN+RRQWAYL
Sbjct: 247 YETQTDQFKIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPSVEGPDFVENSRRQWAYL 306

Query: 318 VEHPDWRETFPKKKPRIFRRAANGKWERS 346
           VEHPDWRETFPKKK RIFRRAANGKWERS
Sbjct: 307 VEHPDWRETFPKKKARIFRRAANGKWERS 335

BLAST of Chy4G080270 vs. NCBI nr
Match: XP_038906106.1 (protein N-terminal asparagine amidohydrolase [Benincasa hispida])

HSP 1 Score: 654 bits (1687), Expect = 4.12e-236
Identity = 313/346 (90.46%), Postives = 331/346 (95.66%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDGAPFT++SSSSNKGADVLYALMEC YLV+ATN FKG PE+RFTVSE+SGVER TM
Sbjct: 1   MIFVDGAPFTVESSSSNKGADVLYALMECHYLVNATNSFKGTPEMRFTVSEQSGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
           SKW+YVFQKEYA VDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDII+IALSQ
Sbjct: 61  SKWIYVFQKEYAIVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIKIALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDP +D ELDVHLVGGFEDVLL+ENNNITR GD KKMEGYS PLCNKII SLWTR
Sbjct: 121 MLSLVVDPASDVELDVHLVGGFEDVLLEENNNITRTGDHKKMEGYSFPLCNKIIESLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PEKFHLQTLCIL+HNTRRDSEGN+YPIFNGF VKTSDGSVFPASFDSTSRCPDE+VRRIR
Sbjct: 181 PEKFHLQTLCILRHNTRRDSEGNAYPIFNGFVVKTSDGSVFPASFDSTSRCPDEVVRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDPSWEGRLLETYETQTDQF+IEPCRW+PWKQ +ALSLQ LSDSEILQSCSTSPS
Sbjct: 241 LSSSYEDPSWEGRLLETYETQTDQFKIEPCRWSPWKQRIALSLQCLSDSEILQSCSTSPS 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERS 346
            EGPDFVE+ARRQWAYLVEHPDWRETFPKKKPRIFRRAA+GKWER+
Sbjct: 301 AEGPDFVESARRQWAYLVEHPDWRETFPKKKPRIFRRAADGKWERN 346

BLAST of Chy4G080270 vs. NCBI nr
Match: XP_022935590.1 (protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita moschata] >KAG6581602.1 Protein N-terminal asparagine amidohydrolase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 613 bits (1581), Expect = 5.85e-220
Identity = 291/346 (84.10%), Postives = 323/346 (93.35%), Query Frame = 0

Query: 1   MIFVDGAPFTLQSSSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGVERPTM 60
           MIFVDG PFT++SSSSNKGADVLYALMECP+LVDAT  FK IPE+RFT SE+ GVER TM
Sbjct: 1   MIFVDGVPFTVESSSSNKGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTM 60

Query: 61  SKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQIALSQ 120
            KWVY+FQKEYATVDPALVDFVGTDEATTCVG+AIRNRKNGMTSVAH+DFP+II+ ALSQ
Sbjct: 61  CKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQ 120

Query: 121 MLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIGSLWTR 180
           MLSLVVDP++DAELDVHLVGGFEDVLLKE+N+IT   DRKK EG+S PLCNKII  LWTR
Sbjct: 121 MLSLVVDPSSDAELDVHLVGGFEDVLLKESNDITELEDRKKTEGHSFPLCNKIIERLWTR 180

Query: 181 PEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEIVRRIR 240
           PE FHLQTLC+L HNTRRDSEGN+YPIF+GF+VKTSDGSVFPASFDSTSRCPDE++RRIR
Sbjct: 181 PETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIR 240

Query: 241 LSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSCSTSPS 300
           LSSSYEDPS +GRLLETYET TDQF+IEPC W+PWK++MALSLQ+LSD+EILQSCSTSPS
Sbjct: 241 LSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPS 300

Query: 301 VEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWERS 346
            E PDFVEN+RRQWAYLVEHPDWRE+FP+KKPRIFRRAA+GKWERS
Sbjct: 301 AEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERS 346

BLAST of Chy4G080270 vs. TAIR 10
Match: AT2G44420.1 (protein N-terminal asparagine amidohydrolase family protein )

HSP 1 Score: 392.9 bits (1008), Expect = 2.7e-109
Identity = 190/350 (54.29%), Postives = 255/350 (72.86%), Query Frame = 0

Query: 1   MIFVDGAPFTLQS-----SSSNKGADVLYALMECPYLVDATNLFKGIPEIRFTVSEESGV 60
           MI+V G  F  +S     SSS++G+ +L  +M  P +  A++ FK + E   +  +ES  
Sbjct: 1   MIYVGGVQFLDESSSFSLSSSSQGSSLLVDVMSHPVITLASDSFKNLEEKNVSF-DESDS 60

Query: 61  ERPTMSKWVYVFQKEYATVDPALVDFVGTDEATTCVGIAIRNRKNGMTSVAHMDFPDIIQ 120
           E  T  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+ IRNRK+GMTSVAHMD P+I+ 
Sbjct: 61  ESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNRKSGMTSVAHMDSPEIVD 120

Query: 121 IALSQMLSLVVDPTADAELDVHLVGGFEDVLLKENNNITRKGDRKKMEGYSLPLCNKIIG 180
           + +SQML LV+    DAELDVH+VGG+EDV +K  + +   GD  K EGYS PLC K++ 
Sbjct: 121 LGISQMLLLVLQDDVDAELDVHMVGGYEDVDIKNADGV---GDYAKPEGYSFPLCCKLVE 180

Query: 181 SLWTRPEKFHLQTLCILQHNTRRDSEGNSYPIFNGFAVKTSDGSVFPASFDSTSRCPDEI 240
           +L  R E FH+QTL IL HNT+ DS+ N+ PIFNG  V TS G++ PASF+ TSRCPDEI
Sbjct: 181 TLQKRRENFHIQTLFILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNRTSRCPDEI 240

Query: 241 VRRIRLSSSYEDPSWEGRLLETYETQTDQFRIEPCRWTPWKQHMALSLQRLSDSEILQSC 300
           VRRIR+SSS+ED SW+G+LL+TY+T+TD+F I PCRWT         L +L+D EIL +C
Sbjct: 241 VRRIRVSSSFEDSSWKGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLTDEEILTNC 300

Query: 301 STSPSVEGPDFVENARRQWAYLVEHPDWRETFPKKKPRIFRRAANGKWER 346
           STSPS EGPDFV + RR W YL+++P+W +TFP+++PR+F R  +G W++
Sbjct: 301 STSPSAEGPDFVNSLRRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346