Chy4G079430 (gene) Cucumber (hystrix) v1

BLAST of Chy4G079430 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.1e-158
Identity = 289/349 (82.81%), Postives = 318/349 (91.12%), Query Frame = 0

Query: 17  SLSLKPPTFSNGAKKFSGVG--LIRASVAVEQQVEKTKVALVRIGTRGSPLALAQAHETR 76
           SLSL   +F   + K S      IRAS+AVEQQ ++ K AL+RIGTRGSPLALAQAHETR
Sbjct: 21  SLSLFTSSFRFSSFKTSPFSKCRIRASLAVEQQTQQNKTALIRIGTRGSPLALAQAHETR 80

Query: 77  DKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVH 136
           DKLMASH ELAE+GAIQIV+IKTTGDKILSQPLADIGGKGLFTKEID+ALINGDIDIAVH
Sbjct: 81  DKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGDIDIAVH 140

Query: 137 SMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIGTASLRRKSQLLNRY 196
           SMKDVPTYLPE+TILPCNLPREDVRDAFISLSA SLA+LPAGS+IGTASLRRKSQ+L+RY
Sbjct: 141 SMKDVPTYLPEETILPCNLPREDVRDAFISLSAASLADLPAGSVIGTASLRRKSQILHRY 200

Query: 197 PSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQG 256
           PSL V +NFRGNVQTRLRKL+EGVV+ATLLALAGL+RLNMTENVTS LSID+MLPAVAQG
Sbjct: 201 PSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLKRLNMTENVTSTLSIDDMLPAVAQG 260

Query: 257 AIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCI 316
           AIGIACRS+DD MA YLASLNHEETRLA+ CERAFL TLDGSCRTPIAGYASRD+DGNC+
Sbjct: 261 AIGIACRSNDDKMAEYLASLNHEETRLAISCERAFLTTLDGSCRTPIAGYASRDKDGNCL 320

Query: 317 FKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGFFDS 364
           F+GLVASPDGTRVLETSR G Y  +DM+ +GKDAG+ELLSRAGPGFF+S
Sbjct: 321 FRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGEELLSRAGPGFFNS 369

BLAST of Chy4G079430 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 3.5e-157
Identity = 279/357 (78.15%), Postives = 314/357 (87.96%), Query Frame = 0

Query: 5   CNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGTRGSP 64
           C++GS+S +GFS   +  P      +K S  G ++A VAVEQ   KT+ A++RIGTRGSP
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRTAIIRIGTRGSP 86

Query: 65  LALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDAL 124
           LALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+AL
Sbjct: 87  LALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEAL 146

Query: 125 INGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIGTASL 184
           ING IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAELPAGS++GTASL
Sbjct: 147 INGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASL 206

Query: 185 RRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSI 244
           RRKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+
Sbjct: 207 RRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSL 266

Query: 245 DEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGY 304
           DEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGY
Sbjct: 267 DEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGY 326

Query: 305 ASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGFF 362
           AS+DE+GNCIF+GLVASPDGT+VLETSR+GPY  +DM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 ASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

BLAST of Chy4G079430 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 2.1e-141
Identity = 252/326 (77.30%), Postives = 287/326 (88.04%), Query Frame = 0

Query: 38  IRASVAVEQQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 97
           +RA+VAV+ + +  KV+L+RIGTRGSPLALAQAHETRDKL A+H ELAE+GA++IV+IKT
Sbjct: 33  VRAAVAVQAEAQ-AKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKT 92

Query: 98  TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 157
           TGD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPRED
Sbjct: 93  TGDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPRED 152

Query: 158 VRDAFISLSAGSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEG 217
           VRDAFI L+A SLAELPAGS++G+ASLRR+SQ+L +YPSLKV+ NFRGNVQTRLRKL EG
Sbjct: 153 VRDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEG 212

Query: 218 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHE 277
            V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIACRS DD M NYL+SLNHE
Sbjct: 213 DVHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHE 272

Query: 278 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYA 337
           +TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR GPY 
Sbjct: 273 DTRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYD 332

Query: 338 IDDMIAMGKDAGQELLSRAGPGFFDS 364
            D M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 FDIMVEMGKDAGHELKAKAGPGFFDS 356

BLAST of Chy4G079430 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.6e-86
Identity = 174/307 (56.68%), Postives = 220/307 (71.66%), Query Frame = 0

Query: 56  VRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGL 115
           +RIGTRGS LALAQAHETRD+LMA+H  LA D A +IVVIKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 116 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPA 175
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    +++ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 176 GSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMT 235
           G+++G++SLRR++QL  R P LK++E FRGNVQTRL+KL +GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 236 ENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDG 295
                 +  DEMLPAVAQG IG+  R+DD   A+ LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 296 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSR 355
           SC+TPIAG A    D     +G +  PDG+ V+   R GP A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 356 AGPGFFD 363
           A   FFD
Sbjct: 311 APADFFD 311

BLAST of Chy4G079430 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.8e-84
Identity = 175/316 (55.38%), Postives = 215/316 (68.04%), Query Frame = 0

Query: 46  QQVEKTKVALVRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 105
           Q  ++T + L  IGTRGSPLALAQAHE RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 106 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 165
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 166 SAGSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLA 225
            AGSL +LPAG+ +GTASLRR++ +L   P LKV  + RGNV+TRLRK++ G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 226 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVC 285
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD +  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 286 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMG 345
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R G  A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 346 KDAGQELLSRAGPGFF 362
            DA  EL  RAG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

BLAST of Chy4G079430 vs. ExPASy TrEMBL
Match: A0A5A7U4C9 (Hydroxymethylbilane synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001480 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.6e-189
Identity = 346/350 (98.86%), Postives = 347/350 (99.14%), Query Frame = 0

Query: 14  GFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGTRGSPLALAQAHET 73
           GFSSLSLKPP FSNGAKKF GVGLIRASVAVEQQVEKTKVALVRIGTRGSPLALAQAHET
Sbjct: 99  GFSSLSLKPPAFSNGAKKFHGVGLIRASVAVEQQVEKTKVALVRIGTRGSPLALAQAHET 158

Query: 74  RDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAV 133
           RDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAV
Sbjct: 159 RDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAV 218

Query: 134 HSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIGTASLRRKSQLLNR 193
           HSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA SLAELPAGS+IGTASLRRKSQLLNR
Sbjct: 219 HSMKDVPTYLPEKTILPCNLPREDVRDAFISLSASSLAELPAGSMIGTASLRRKSQLLNR 278

Query: 194 YPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQ 253
           YPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQ
Sbjct: 279 YPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQ 338

Query: 254 GAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNC 313
           GAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNC
Sbjct: 339 GAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNC 398

Query: 314 IFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGFFDS 364
           IFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGFFDS
Sbjct: 399 IFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGFFDS 448

BLAST of Chy4G079430 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 3.6e-189
Identity = 344/363 (94.77%), Postives = 349/363 (96.14%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           M RPCN+GSLS  GFSSLSLKPP FS G KKF G GLIRASVAVEQQ EKTKVALVRIGT
Sbjct: 14  MARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQAEKTKVALVRIGT 73

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 74  RGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 133

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS GSLAELPAGS+IG
Sbjct: 134 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSVGSLAELPAGSVIG 193

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTE VTS
Sbjct: 194 TASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTETVTS 253

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 254 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 313

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAG+ELLSRAGPGF
Sbjct: 314 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGKELLSRAGPGF 373

Query: 361 FDS 364
           FDS
Sbjct: 374 FDS 376

BLAST of Chy4G079430 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 4.8e-189
Identity = 343/363 (94.49%), Postives = 350/363 (96.42%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           M RPCN+GSLS  GFSSLSLKPP FS G K+F GVGLIRASVAVEQQ EKTKVALVRIGT
Sbjct: 14  MARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQAEKTKVALVRIGT 73

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 74  RGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 133

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS GSLAELPAGS+IG
Sbjct: 134 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSVGSLAELPAGSVIG 193

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTE VTS
Sbjct: 194 TASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTETVTS 253

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 254 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 313

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDM+AMGKDAG+ELLSRAGPGF
Sbjct: 314 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMVAMGKDAGKELLSRAGPGF 373

Query: 361 FDS 364
           FDS
Sbjct: 374 FDS 376

BLAST of Chy4G079430 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.1e-188
Identity = 343/363 (94.49%), Postives = 351/363 (96.69%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           M RPCN+ SLSL GFSSLSLKPP F+N  KKF G GLIRASVAVEQQ EKTKVAL+RIGT
Sbjct: 14  MARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQPEKTKVALLRIGT 73

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 74  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 133

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL AGSLAELPAGS++G
Sbjct: 134 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVG 193

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSL+VLENFRGNVQTRL+KLNEGVVQATLLALAGLRRLNMTENVTS
Sbjct: 194 TASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTS 253

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 254 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 313

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRG YAIDDMIAMGKDAG+ELLSRAGPGF
Sbjct: 314 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGF 373

Query: 361 FDS 364
           FDS
Sbjct: 374 FDS 376

BLAST of Chy4G079430 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.8e-188
Identity = 340/363 (93.66%), Postives = 351/363 (96.69%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           M RPCNVGSLS LGFSSLSLKPP F NG KKF G+GL+RASVAVEQQ EKTKVALVRIGT
Sbjct: 14  MARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQTEKTKVALVRIGT 73

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQA+ETRDKLMA HPELAEDGAIQI++IKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 74  RGSPLALAQAYETRDKLMAIHPELAEDGAIQIIIIKTTGDKILSQPLADIGGKGLFTKEI 133

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGS+IG
Sbjct: 134 DDALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSVIG 193

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS
Sbjct: 194 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 253

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           +LSIDEMLPAVAQGAIGIACRS+DD MANYLASLNHEETRLAVVCERAFLE LDGSCRTP
Sbjct: 254 VLSIDEMLPAVAQGAIGIACRSNDDKMANYLASLNHEETRLAVVCERAFLEMLDGSCRTP 313

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYAS+DEDGNCIFKGLVASPDGTRVLETSRRG YAIDDM+AMGKDAG+ELLSRAGPGF
Sbjct: 314 IAGYASKDEDGNCIFKGLVASPDGTRVLETSRRGAYAIDDMVAMGKDAGKELLSRAGPGF 373

Query: 361 FDS 364
           FDS
Sbjct: 374 FDS 376

BLAST of Chy4G079430 vs. NCBI nr
Match: XP_004149732.1 (porphobilinogen deaminase, chloroplastic [Cucumis sativus])

HSP 1 Score: 691 bits (1782), Expect = 7.17e-250
Identity = 356/363 (98.07%), Postives = 358/363 (98.62%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           MPRPCNVGSLS LGFSSLSLKPPTFSNGAKKF GVGLIRA VA EQQVEKTKVAL+RIGT
Sbjct: 14  MPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKTKVALLRIGT 73

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 74  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 133

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGS AELPAGSIIG
Sbjct: 134 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIG 193

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS
Sbjct: 194 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 253

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 254 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 313

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKDAGQELLSRAGPGF
Sbjct: 314 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGF 373

Query: 361 FDS 363
           FDS
Sbjct: 374 FDS 375

BLAST of Chy4G079430 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 689 bits (1777), Expect = 4.30e-249
Identity = 355/363 (97.80%), Postives = 357/363 (98.35%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           MPRP NVGSLSLLGFSSLSLKPP FSNGAKKF G GLIRASVAVEQQ EKTKVALVRIGT
Sbjct: 14  MPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQTEKTKVALVRIGT 73

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 74  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 133

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG
Sbjct: 134 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 193

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS
Sbjct: 194 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 253

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACR+DDD MANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 254 ILSIDEMLPAVAQGAIGIACRTDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 313

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAG+ELLSRAGPGF
Sbjct: 314 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGKELLSRAGPGF 373

Query: 361 FDS 363
           FDS
Sbjct: 374 FDS 376

BLAST of Chy4G079430 vs. NCBI nr
Match: KGN58748.2 (hypothetical protein Csa_002701 [Cucumis sativus])

HSP 1 Score: 691 bits (1782), Expect = 5.77e-249
Identity = 356/363 (98.07%), Postives = 358/363 (98.62%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           MPRPCNVGSLS LGFSSLSLKPPTFSNGAKKF GVGLIRA VA EQQVEKTKVAL+RIGT
Sbjct: 70  MPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKTKVALLRIGT 129

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 130 RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 189

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGS AELPAGSIIG
Sbjct: 190 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIG 249

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS
Sbjct: 250 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 309

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 310 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 369

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI+DMIAMGKDAGQELLSRAGPGF
Sbjct: 370 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGF 429

Query: 361 FDS 363
           FDS
Sbjct: 430 FDS 431

BLAST of Chy4G079430 vs. NCBI nr
Match: XP_023523547.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672 bits (1735), Expect = 2.04e-242
Identity = 345/363 (95.04%), Postives = 350/363 (96.42%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           M RPCN+GSLS  GFSSLSLKPP FS GAKKF G GLIRASVAVEQQ EKTKVALVRIGT
Sbjct: 31  MARPCNLGSLSRHGFSSLSLKPPAFSTGAKKFHGFGLIRASVAVEQQAEKTKVALVRIGT 90

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 91  RGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 150

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS GSLAELPAGS+IG
Sbjct: 151 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSVGSLAELPAGSVIG 210

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTE VTS
Sbjct: 211 TASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTETVTS 270

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 271 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 330

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAG+ELLSRAGPGF
Sbjct: 331 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGKELLSRAGPGF 390

Query: 361 FDS 363
           FDS
Sbjct: 391 FDS 393

BLAST of Chy4G079430 vs. NCBI nr
Match: XP_022949290.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 671 bits (1731), Expect = 4.40e-242
Identity = 344/363 (94.77%), Postives = 349/363 (96.14%), Query Frame = 0

Query: 1   MPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGT 60
           M RPCN+GSLS  GFSSLSLKPP FS G KKF G GLIRASVAVEQQ EKTKVALVRIGT
Sbjct: 14  MARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQAEKTKVALVRIGT 73

Query: 61  RGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEI 120
           RGSPLALAQAHETR+KLMA HPELAEDGAIQIV+IKTTGDKILSQPLADIGGKGLFTKEI
Sbjct: 74  RGSPLALAQAHETREKLMAIHPELAEDGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEI 133

Query: 121 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIG 180
           DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLS GSLAELPAGS+IG
Sbjct: 134 DDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSVGSLAELPAGSVIG 193

Query: 181 TASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTS 240
           TASLRRKSQLLNRYPSL VLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTE VTS
Sbjct: 194 TASLRRKSQLLNRYPSLNVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTETVTS 253

Query: 241 ILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 300
           ILSIDEMLPAVAQGAIGIACRSDDD MANYLASLNHEETRLAVVCERAFLETLDGSCRTP
Sbjct: 254 ILSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAVVCERAFLETLDGSCRTP 313

Query: 301 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGF 360
           IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAG+ELLSRAGPGF
Sbjct: 314 IAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGKELLSRAGPGF 373

Query: 361 FDS 363
           FDS
Sbjct: 374 FDS 376

BLAST of Chy4G079430 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 555.8 bits (1431), Expect = 2.5e-158
Identity = 279/357 (78.15%), Postives = 314/357 (87.96%), Query Frame = 0

Query: 5   CNVGSLSLLGFSSLSLKPPTFSNGAKKFSGVGLIRASVAVEQQVEKTKVALVRIGTRGSP 64
           C++GS+S +GFS   +  P      +K S  G ++A VAVEQ   KT+ A++RIGTRGSP
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ---KTRTAIIRIGTRGSP 86

Query: 65  LALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDAL 124
           LALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+AL
Sbjct: 87  LALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEAL 146

Query: 125 INGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSLAELPAGSIIGTASL 184
           ING IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A +LAELPAGS++GTASL
Sbjct: 147 INGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASL 206

Query: 185 RRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSI 244
           RRKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+
Sbjct: 207 RRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSL 266

Query: 245 DEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGY 304
           DEMLPAVAQGAIGIACR+DDD MA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGY
Sbjct: 267 DEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGY 326

Query: 305 ASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIDDMIAMGKDAGQELLSRAGPGFF 362
           AS+DE+GNCIF+GLVASPDGT+VLETSR+GPY  +DM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 ASKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380