Chy4G078390 (gene) Cucumber (hystrix) v1

Overview
NameChy4G078390
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description3-oxo-Delta(4,5)-steroid 5-beta-reductase
LocationchrH04: 17323773 .. 17326104 (-)
RNA-Seq ExpressionChy4G078390
SyntenyChy4G078390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTGGTGGTGGGCCGGAGCAATCGGTGCTGCTAAGGTAAAACTCTACAACCCCAATCCCTTTTCTTCTCAACTCTATCCATCTTTTATTCCTCTTCTCTCTTCCAATCTTGCAGAAGAAATTTGAAGAAGATGAACCTCCCAGGAGCTTTGAGAGCGTAGCTCTGGTGATTGGCGTCACTGGTATCGTCGGCAATAGTCTTGCCGAGATTCTCCCTCTTTCCGATACCCCCGGTGGTCCATGGAAGGTCTACGGCGTCGCTCGCAGGCCGAGGCCAGCCTGGAATGCTGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCCCCAAGACGCCGAAACCAAACTCTCGCAGCTCGCTGATGTCACCCATCTCTTCTATGTCACATGGACCAATCGAACCGCTGAGATCGAAAACTGTGAGGCCAACGTTAAAATGCTTCGCAACGTTCTGCGATCAGTGATCCCCAACGCACCCAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACGTTGGGTCGTTCGAGAGCATTATCAATAAATCGTCGCAGCGGCATGATCCTCCTTTCACAGAGGATTTGCCTCGATTGGAATGCCCAAATTTCTATTACAAACAAGAAGATTTGTTATGGGAAGAGATTGAACAATCTCAGAAAAAGGATCTGACGTGGGCGGTGATTCGACCCAATGTAATTTTCGGGTTTTCCCCTTTCAGCTTGATGAACATTGTCGGAACCCTATGTGTCTACGCTGCCATATGCAAACACGAGGGACGTCCGTTGAAATTCCCCGGCACTAAATTGGCTTGGGAGGATTTCCAGGCGGCGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTATTCAAAAAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAACACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATTGAGGAATATGGGTTCGATGGAGAAGGGGAGAGATTGACACTGGTGGATTTGATGAAGGACAAAAGTGACGTATGGGATGAGATTGTGAAGGAGAATCAATTGCAGCAAACCAAATTGGAGGAGATTGGTACTTGGTGGTTTGTAGATGCAATTTTCAGCGTCTCAGGTAACATAGATAGTATGAACAAGAGCAAAGAACATGGGTTTTTGGGCTTCAGGAACTCTAAGAACTCTTTCATTTCTTGGATTGACAAGATTAAGGCTTTCAAAATTGTGCCTTGATTGGCCTTGTAATTCTTGTAGAACTGTTTCATTTGGTTTTATCTGTTTTTATTCCTGGTTTTGCAATAAGCTTGATCACAAGAAAGTGTTGCTTTGTTTACTTTCTTGCTATTTCATAAAGTGATTGTAAAGTGATTGAGTTATTTGTGGTTGAAGTCTATAAAGATAATGGAAAATTATTTGTGAGTGTTATCTAGCATTCTAGATAGTGCTAAAGAATATTAAAATGAGATGCATGGTTAAACAGTCTCAGTGTCACATCACGAATAGGTTGCTAAATTGGATTTCCATTATCTCTTACAGATGTCAAAGTTATTGGAAATTCTAAGTAGTGGACTTCCCCATTGTCCGTCCTAACTTTCACTCTCTTAACTAACTCTTTCCACTGAGAAGAGTTTTCATTTATGTTGTTATTCAAGTTAATTCTAAAACTAAGATCTCTGTATATTATTTGGCTTAACTACAACAAATAATATTTTCAACAGGGTCCTTTTTTATAGCATTAGACTGAAAATGCTATGGAAAAATTGGAAGAAAAAAAAGGCGCCTCGTCCTATTTTATAACTTTTAGTTTAGACTGAAAGTGAGGTTTTTTTTCACAATTATGGCCGCTTCTCTTACGAGTGGATGTAGCAATGTAGCAGCTCTGGCGGAGTTGTGAAGGGTTGTAAGGGAGATGTTTGATCGGAGTTTAAATGTTCATGACTCTGGAGGCGACAGGTGGACGGGGCAGAGGAGGAATGGCGTAATCTTAGATGGTTTTAGTGAAGTTTATTAATTTTGCTTAATATGCTGAGACCACCAAGCCTTCCTCTACATTTCTTCCCTCCAGAATTCAAAATCTGCGTAATCTTAGTCTCTCTCTCTCTCTCTGTACTTATTTGCTAAAAAACGTAATTGTTAAAGTTTCTTGCTCTATACAGATTTGGATACAGAGGATGCGGGAAAATGCTAATCAAAGGTACATAAGATGGTGAATTAGAGTTCATAAGTTTTGTTGACTATTTTATTTTCAGAGATGTGGAAGAATATGAGGGTTTAAAAGCTAAATCTCCAGATGAGAGCTGA

mRNA sequence

ATGAGCTGGTGGTGGGCCGGAGCAATCGGTGCTGCTAAGAAGAAATTTGAAGAAGATGAACCTCCCAGGAGCTTTGAGAGCGTAGCTCTGGTGATTGGCGTCACTGGTATCGTCGGCAATAGTCTTGCCGAGATTCTCCCTCTTTCCGATACCCCCGGTGGTCCATGGAAGGTCTACGGCGTCGCTCGCAGGCCGAGGCCAGCCTGGAATGCTGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCCCCAAGACGCCGAAACCAAACTCTCGCAGCTCGCTGATGTCACCCATCTCTTCTATGTCACATGGACCAATCGAACCGCTGAGATCGAAAACTGTGAGGCCAACGTTAAAATGCTTCGCAACGTTCTGCGATCAGTGATCCCCAACGCACCCAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACGTTGGGTCGTTCGAGAGCATTATCAATAAATCGTCGCAGCGGCATGATCCTCCTTTCACAGAGGATTTGCCTCGATTGGAATGCCCAAATTTCTATTACAAACAAGAAGATTTGTTATGGGAAGAGATTGAACAATCTCAGAAAAAGGATCTGACGTGGGCGGTGATTCGACCCAATGTAATTTTCGGGTTTTCCCCTTTCAGCTTGATGAACATTGTCGGAACCCTATGTGTCTACGCTGCCATATGCAAACACGAGGGACGTCCGTTGAAATTCCCCGGCACTAAATTGGCTTGGGAGGATTTCCAGGCGGCGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTATTCAAAAAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAACACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATTGAGGAATATGGGTTCGATGGAGAAGGGGAGAGATTGACACTGGTGGATTTGATGAAGGACAAAAGTGACGTATGGGATGAGATTGTGAAGGAGAATCAATTGCAGCAAACCAAATTGGAGGAGATTGGTACTTGGTGGTTTGTAGATGCAATTTTCAGCGTCTCAGATGTCAAAATTTGGATACAGAGGATGCGGGAAAATGCTAATCAAAGAGATGTGGAAGAATATGAGGGTTTAAAAGCTAAATCTCCAGATGAGAGCTGA

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATCGGTGCTGCTAAGAAGAAATTTGAAGAAGATGAACCTCCCAGGAGCTTTGAGAGCGTAGCTCTGGTGATTGGCGTCACTGGTATCGTCGGCAATAGTCTTGCCGAGATTCTCCCTCTTTCCGATACCCCCGGTGGTCCATGGAAGGTCTACGGCGTCGCTCGCAGGCCGAGGCCAGCCTGGAATGCTGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCCCCAAGACGCCGAAACCAAACTCTCGCAGCTCGCTGATGTCACCCATCTCTTCTATGTCACATGGACCAATCGAACCGCTGAGATCGAAAACTGTGAGGCCAACGTTAAAATGCTTCGCAACGTTCTGCGATCAGTGATCCCCAACGCACCCAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACGTTGGGTCGTTCGAGAGCATTATCAATAAATCGTCGCAGCGGCATGATCCTCCTTTCACAGAGGATTTGCCTCGATTGGAATGCCCAAATTTCTATTACAAACAAGAAGATTTGTTATGGGAAGAGATTGAACAATCTCAGAAAAAGGATCTGACGTGGGCGGTGATTCGACCCAATGTAATTTTCGGGTTTTCCCCTTTCAGCTTGATGAACATTGTCGGAACCCTATGTGTCTACGCTGCCATATGCAAACACGAGGGACGTCCGTTGAAATTCCCCGGCACTAAATTGGCTTGGGAGGATTTCCAGGCGGCGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTATTCAAAAAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAACACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATTGAGGAATATGGGTTCGATGGAGAAGGGGAGAGATTGACACTGGTGGATTTGATGAAGGACAAAAGTGACGTATGGGATGAGATTGTGAAGGAGAATCAATTGCAGCAAACCAAATTGGAGGAGATTGGTACTTGGTGGTTTGTAGATGCAATTTTCAGCGTCTCAGATGTCAAAATTTGGATACAGAGGATGCGGGAAAATGCTAATCAAAGAGATGTGGAAGAATATGAGGGTTTAAAAGCTAAATCTCCAGATGAGAGCTGA

Protein sequence

MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANVKMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNFYYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFNIEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVSDVKIWIQRMRENANQRDVEEYEGLKAKSPDES*
Homology
[Close]
BLAST of Chy4G078390 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 4.2e-164
Identity = 265/355 (74.65%), Postives = 302/355 (85.07%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP WNADHPI+YIQCDVSD +D  +KLS L DVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F    N    RHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QED+L+EEI++ +   +TW++ RPN+IFGFSP+SLMNIVGTLCVYAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSV 356
           IEEYGF+ EG+ L LV++MK K  VW+E+VKENQLQ+ KLEE+G WWF D I  V
Sbjct: 301 IEEYGFE-EGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV 349

[Close]
BLAST of Chy4G078390 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.1e-151
Identity = 248/368 (67.39%), Postives = 294/368 (79.89%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP+WN DHPI YI CDV D  D E KLS L DVTH+FY TWT R+ E ENCEAN 
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHY+G+FE +    S+ HDPP TEDLPRL+  N+
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDL---KSKCHDPPLTEDLPRLDSQNY 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QED+L+EE++  +K+ LTW+V RP  IFGFSP+S+MN+VGTLCVYAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQ--KKEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+ +   SDADLIAE  IW AVDPY+KNEAFN +NGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVSDVKI 360
           + E G   EG+++ L DLMKDK  VWD+IV+EN L  TKLE++G WWF D +      + 
Sbjct: 301 V-ECGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG---NEC 359

Query: 361 WIQRMREN 369
           W+  M ++
Sbjct: 361 WLDTMNKS 359

[Close]
BLAST of Chy4G078390 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 2.6e-150
Identity = 247/368 (67.12%), Postives = 292/368 (79.35%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTP GPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP+WN DHPI YI CDV D  D E KLS L DVTH+FY TWT R+ E ENCEAN 
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHY+G+FE   +   +RHDPP TEDLPRL+  NF
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFE---DWKIKRHDPPLTEDLPRLDSQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QED+L+EE++  +K+ LTW+V RP  IFGFSP+S+MN+VGTLCVYAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQ--KKESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+ +   SDADLIAE  IW AVDPY+KNEAFN +NGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVSDVKI 360
           + E G   EG+ + L DLMKDK  VWD+IV+EN L  TKLE++G WWF D +      + 
Sbjct: 301 V-ECGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG---NEC 359

Query: 361 WIQRMREN 369
           W+  M ++
Sbjct: 361 WLDTMNKS 359

[Close]
BLAST of Chy4G078390 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 4.5e-150
Identity = 247/353 (69.97%), Postives = 280/353 (79.32%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA+KKFEED+ PR  +SVAL+IGVTGIVGNSLAEILP+SDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRPAWNADHP+EYIQCD+SD  D  TKLS L DVTH+F+VTW NR  E E CE N 
Sbjct: 61  VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVL ++IP A NL HICLQTG KHY+G FE+      + H+PPFTED+PRL  PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAF--GKIKPHEPPFTEDMPRLNAPNF 180

Query: 181 YYKQEDLLWEEIEQSQKK-DLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPL 240
           YY  ED+L   +E S+KK  L W+V RP VIFGFSPFS+MNI+GTLCVYAAICKHE  PL
Sbjct: 181 YYTLEDML---VEASEKKAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPL 240

Query: 241 KFPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQF 300
           KFPGTK AW  +  ASDADLIAE  IW AVDPY+KNEAFNC+NGD+FKWKH WKVLAEQF
Sbjct: 241 KFPGTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWKVLAEQF 300

Query: 301 NIEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAI 353
            +E   FD   +  +LV+ MKDK  VW+EIV+EN L  TKLE + TWWF D I
Sbjct: 301 GVEYAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVI 348

[Close]
BLAST of Chy4G078390 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 8.2e-144
Identity = 238/352 (67.61%), Postives = 279/352 (79.26%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARR RPAW+ D+PI Y+QCD+SDP D++ KLS L DVTH+FYVTW NR+ E ENCEAN 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G FES      + HDPP+TEDLPRL+  NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY  ED++ EE+E  +K+ LTW+V RP  IFGFSP+S+MN+VGTLCVYAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVE--KKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           F G K AW+ +   SDADLIAE HIW AVDPY+KNEAFN +NGDVFKWKHFWKVLAEQF 
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAI 353
           +   G   EG  L L DLMK K  VW+EIV+EN L  TKL+++G WWF D I
Sbjct: 301 V-GCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVI 347

[Close]
BLAST of Chy4G078390 vs. ExPASy TrEMBL
Match: A0A0A0L8Y0 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 6.2e-211
Identity = 347/356 (97.47%), Postives = 351/356 (98.60%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EIENCEANV
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YYKQEDLLWEEIEQSQKKDLTWAVIRPN+IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG KLAWEDFQ ASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 357
           IEEYGFD EGE LTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVD+IFS+S
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMS 356

[Close]
BLAST of Chy4G078390 vs. ExPASy TrEMBL
Match: A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 7.1e-207
Identity = 342/356 (96.07%), Postives = 350/356 (98.31%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP WNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EI+NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES+INKSSQRH+PPFTEDLPRLECPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YYKQEDLL+EEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGTKLAWEDFQAASDADL+AEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 357
           IEEYGF+ EGERLTLVDLMKDKSDVWDEIVKENQLQ TKLEEIG WW+VD IFSVS
Sbjct: 301 IEEYGFE-EGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVS 355

[Close]
BLAST of Chy4G078390 vs. ExPASy TrEMBL
Match: A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 7.1e-207
Identity = 342/356 (96.07%), Postives = 350/356 (98.31%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP WNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EI+NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES+INKSSQRH+PPFTEDLPRLECPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YYKQEDLL+EEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGTKLAWEDFQAASDADL+AEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 357
           IEEYGF+ EGERLTLVDLMKDKSDVWDEIVKENQLQ TKLEEIG WW+VD IFSVS
Sbjct: 301 IEEYGFE-EGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVS 355

[Close]
BLAST of Chy4G078390 vs. ExPASy TrEMBL
Match: A0A6J1F7T0 (uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC111442928 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.2e-190
Identity = 311/356 (87.36%), Postives = 333/356 (93.54%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+ E+ENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS Q HD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QEDLL+EEIE++Q+KDLTWAVIRPNVIFGFSPF  MNI+GTLC YAAIC+HEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFGWMNIIGTLCAYAAICRHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE+F  ASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 357
           IE+YGF+ EGE+LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+S
Sbjct: 301 IEDYGFE-EGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSIS 355

[Close]
BLAST of Chy4G078390 vs. ExPASy TrEMBL
Match: A0A6J1IQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111477228 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.6e-188
Identity = 308/356 (86.52%), Postives = 330/356 (92.70%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK F+EDEPPR FE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKTFDEDEPPRRFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+ E+ENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVI NAPNLRHICLQTGTKHYVGSFESI+NKS Q    PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVISNAPNLRHICLQTGTKHYVGSFESIVNKSVQPPGSPFTEDLPRLKCPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QEDLL+EEIE++Q+KDLTWAVIRPNVIFGFSPFS MNI+GTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFSWMNIIGTLCAYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE+F  ASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 357
           IE+YGF+ EGE+LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+S
Sbjct: 301 IEDYGFE-EGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSIS 355

[Close]
BLAST of Chy4G078390 vs. NCBI nr
Match: XP_004152001.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothetical protein Csa_017526 [Cucumis sativus])

HSP 1 Score: 738 bits (1906), Expect = 5.07e-268
Identity = 347/356 (97.47%), Postives = 351/356 (98.60%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EIENCEANV
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YYKQEDLLWEEIEQSQKKDLTWAVIRPN+IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG KLAWEDFQ ASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 356
           IEEYGFD EGE LTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVD+IFS+S
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMS 356

[Close]
BLAST of Chy4G078390 vs. NCBI nr
Match: XP_008447374.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo var. makuwa])

HSP 1 Score: 725 bits (1871), Expect = 1.05e-262
Identity = 342/356 (96.07%), Postives = 350/356 (98.31%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP WNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EI+NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES+INKSSQRH+PPFTEDLPRLECPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YYKQEDLL+EEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGTKLAWEDFQAASDADL+AEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 356
           IEEYGF+ EGERLTLVDLMKDKSDVWDEIVKENQLQ TKLEEIG WW+VD IFSVS
Sbjct: 301 IEEYGFE-EGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVS 355

[Close]
BLAST of Chy4G078390 vs. NCBI nr
Match: XP_038899926.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida])

HSP 1 Score: 681 bits (1758), Expect = 1.73e-245
Identity = 319/356 (89.61%), Postives = 336/356 (94.38%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           +ARRPRP WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWTNR  EIENCEANV
Sbjct: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GS +SI++KS Q HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QEDLL+EEIEQSQKKDLTWAVIRPNVIFGFSPFSL+NIVGTLCVYAAICKHEGR LK
Sbjct: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEDFQ  SDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 356
           IE+YGF+ +GERLTLV+LMKDKS+VWDEIVKENQLQ TKLEEI  WWF D IFS+S
Sbjct: 301 IEDYGFE-DGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLS 355

[Close]
BLAST of Chy4G078390 vs. NCBI nr
Match: XP_023535925.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 675 bits (1741), Expect = 6.74e-243
Identity = 313/356 (87.92%), Postives = 335/356 (94.10%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+ E+ENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS QRHD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQRHDSPFTEDLPRLKCPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QEDLL+EEIE++Q+KDLTWAVIRPNVIFGFSPFS MNI+GTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFSWMNIIGTLCAYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE+F  ASDADLIAEQHIWT+VDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSIASDADLIAEQHIWTSVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 356
           IE+YGF+ EGE+LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+S
Sbjct: 301 IEDYGFE-EGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSIS 355

[Close]
BLAST of Chy4G078390 vs. NCBI nr
Match: KAG6591410.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 670 bits (1728), Expect = 6.44e-241
Identity = 312/356 (87.64%), Postives = 332/356 (93.26%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARR RP+WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWT R+ E+ENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESI+NKS Q HD PFTEDLPRL+CPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QEDLL EEI ++Q+KDLTWAVIRPNVIFGFSPFS MNI+GTLC YAAICKHEGRPLK
Sbjct: 181 YYPQEDLLLEEIVKTQRKDLTWAVIRPNVIFGFSPFSWMNIIGTLCAYAAICKHEGRPLK 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE+F  ASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSVS 356
           IE+YGF+ EGE+LTLV+LMKDKS VW+EIVKENQLQ T+LEEIG WWFVD I S+S
Sbjct: 301 IEDYGFE-EGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSIS 355

[Close]
BLAST of Chy4G078390 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 578.9 bits (1491), Expect = 3.0e-165
Identity = 265/355 (74.65%), Postives = 302/355 (85.07%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP WNADHPI+YIQCDVSD +D  +KLS L DVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F    N    RHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QED+L+EEI++ +   +TW++ RPN+IFGFSP+SLMNIVGTLCVYAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSV 356
           IEEYGF+ EG+ L LV++MK K  VW+E+VKENQLQ+ KLEE+G WWF D I  V
Sbjct: 301 IEEYGFE-EGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV 349

[Close]
BLAST of Chy4G078390 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 572.4 bits (1474), Expect = 2.8e-163
Identity = 264/355 (74.37%), Postives = 301/355 (84.79%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA KK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAA-KKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTAEIENCEANV 120
           VARRPRP WNADHPI+YIQCDVSD +D  +KLS L DVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F    N    RHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
           YY QED+L+EEI++ +   +TW++ RPN+IFGFSP+SLMNIVGTLCVYAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKLAWEDFQAASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEEYGFDGEGERLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIFSV 356
           IEEYGF+ EG+ L LV++MK K  VW+E+VKENQLQ+ KLEE+G WWF D I  V
Sbjct: 301 IEEYGFE-EGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV 348

[Close]
BLAST of Chy4G078390 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.4e-58
Identity = 127/335 (37.91%), Postives = 189/335 (56.42%), Query Frame = 0 

Query: 26  ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPAWNADHPIEYIQCDVSDP 85
           E+VAL+ GVTG+VG  + + L L   PG  W++YGVAR P    +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVARNPE-INSMTKMYNFISCDLLNA 75

Query: 86  QDAETKLSQLAD-VTHLFYVTWTNR--TAEIENCEANVKMLRNVLRSVIPNAPNLRHICL 145
            + + +LS L D V+H+F+VTW+        E C  N  ML N L +++PNA  L+H  L
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135

Query: 146 QTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLEC-PNFYYKQEDLLWEEIEQSQKKDLT 205
           QTG KHYV   E  + +        ++E+ PR     NFYY  EDLL E+I +S    + 
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRS---SVV 195

Query: 206 WAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFPGTKLAWED-FQAASDADLI 265
           W+V RP ++ G S  +L N +G+LCVY A+CK+   P  F GT+  WE+ +   SD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255

Query: 266 AEQHIWTAVD--PYSKNEAFNCNNGDVFKWKHFWKVLAEQFNIEEYGFDGEGERLTLVDL 325
           AEQHI+ A       K EAFN  NG  F WK  W  + ++  ++        E       
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGRE 315

Query: 326 MKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDAIF 354
           M ++  VWDEIV + +L +T++E++  W+F+DA+F
Sbjct: 316 MVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALF 343

The following BLAST results are available for this feature:
BLAST of Chy4G078390 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9STX24.2e-16474.653-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
A0A221J5W81.1e-15167.39(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ... [more]
A0A221J5X32.6e-15067.12(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1... [more]
A0A1C9CX664.5e-15069.97(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... [more]
Q6PQJ98.2e-14467.613-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1[more]
BLAST of Chy4G078390 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8Y06.2e-21197.47Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 P... [more]
A0A5A7T3B37.1e-20796.073-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BHA77.1e-20796.073-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983... [more]
A0A6J1F7T01.2e-19087.36uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC1114429... [more]
A0A6J1IQC19.6e-18886.523-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
BLAST of Chy4G078390 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004152001.15.07e-26897.473-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothet... [more]
XP_008447374.11.05e-26296.07PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954.... [more]
XP_038899926.11.73e-24589.613-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida][more]
XP_023535925.16.74e-24387.923-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo][more]
KAG6591410.16.44e-24187.643-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp... [more]
BLAST of Chy4G078390 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G24220.13.0e-16574.65NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.22.8e-16374.37NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G58750.11.4e-5837.91NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 29..256
e-value: 2.7E-5
score: 23.8
NoneNo IPR availableGENE3D3.40.50.720coord: 25..382
e-value: 3.5E-125
score: 419.7
NoneNo IPR availablePANTHERPTHR32487:SF203-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE-LIKEcoord: 1..358
NoneNo IPR availablePANTHERPTHR324873-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 1..358
NoneNo IPR availableCDDcd089485beta-POR_like_SDR_acoord: 28..353
e-value: 5.64046E-132
score: 378.897
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 28..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G078390.1Chy4G078390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009611 response to wounding
biological_process GO:0008202 steroid metabolic process
biological_process GO:0010051 xylem and phloem pattern formation
molecular_function GO:0047568 3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function GO:0035671 enone reductase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0003824 catalytic activity