Chy4G078160 (gene) Cucumber (hystrix) v1

Overview
NameChy4G078160
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionLong-chain-alcohol oxidase
LocationchrH04: 16956116 .. 16959023 (+)
RNA-Seq ExpressionChy4G078160
SyntenyChy4G078160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGATACAGAAGTTCCATCCCACCCTTTATTGAGAAGACGAAGAACAGACCTCAACCATGACTTCTCTTCTTCTCAAATGGAAGCCATGGCTGCCTTCTGTGAAGCCCTCATTCCCCCTGTGCCTCTTTTTAAAGAAACCCCACTTGATCAATCTCTTCTTTCATTCTACCAATCCTCTGCTTTTCAAGCTCCAATCCCAGATGAGGTTATATTTTTACTTTGAATTCCTTTTAAGCTTTTATTCCTTCTTGTACTGAGAACTTGTTTGTTTGTTTGTTCATATGGATTTTTAGGTAGCTGACCTATTGGTGGAGAGGGCTAATCCAAAAGCTGTGTTTCTTGTGAAATTGGTTCTGAGACTTCTCTCTTTTAGAATTGGGACATTGTTGCTTTGCGGGAATGTTTGTTTTGATTGGAGATGGCCTTTCTTTCTTAAATTTTCTGAGATTTCTTTGGAGAAGAGAGAACGAATTCTCAAGAATTGGTCTATGGCTCACCACAAGTATACTGTACTTTTGAGAATGACTTTCATGATTATTAAAATTTTCTGCTGCTTCAAATTCTTCTCCAGGGTAACTTTTTCTTTCAATTGTACTCTCAGGTTTCTTTTTACTATAATTTTGTTTTGGTGTTCATATTCAACATTGCTTTAGAATATTCTGTTATGATTGTTAGTTTAACATTTCTTCTGCTCTGTTAACAATTTGCTTTAAGAACATCTTGTCCTGTATGTTAACGCTTTTCTTTTTAGTTTTAAATTTTCTCTCTTTTTTTTCACTTTTTTTTTTCAAAAACTAAGTGTATAAATACCATTTCCATCCATAAGTTTCTTATTTCTGATGTAGAAAGTTTTCATAATGTTGAAAACTAAGCTTGTTTCCTTTTTGGAATTTGGCTAAATGTTTCCTCAAGAAATGTGAAAGCCGTAGAAAAGAAATTAGAAGAAACAAGCACAATTTTCGAACAGTAAAACAAAAAACGTAAAAGTACGAAAGTGTTATCCAAGGGGGCCTTACTCATTTCTTTTCCAGTTGGCCCCTTCACTATTTGTTTTCATCTATATTGGCATTTATAGGGTTCCTCTTTTTGAATATTTAGTTAGCAATTATAGGGTTCCTCTTTTTGGTATTGACTATTGAGTACTATTATACAGAGGATTCTATTCTGTTTTGTTTCAGATCGATGAGGGAACGAAAAACGTTGCATGGAAAGGGATGGGGTATAAAGTAGACACCCGAAAACAGTTGAACCATACCCAAAAAGAGCGGCCTCTTGAAAGGGGAATGATAGAAACTGGCAATGAAGATGATTCCACTCTTCTCCGGTCTCTTTCACAGAAAGGCCTTGAAGTAATTGAAGATAAAAAACATGGTAATGCCTATAAGATCAAATGTGATCTTGTGATTGTTGGCTCGGGTTGTGGTGGTGGAGTGGCTGCTGCGGTTCTTGCAAAATCGGGTTTGAAGGTAGTCGTTCTCGAGAAAGGGAACTATTTCGTGGCTGAAGATTATTCTTCCCTAGAAGGTCCATCCATGAATGAACTATATGAGTCAGGTGGACTCAAGTCAACTCTCGATGGGAAGGTTATGCTGTTGGCTGGAACTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCAATTAGAACTCCAAAATCCATTCTCGATGAATGGTCTACTGATTATAAAATTCCTTTGTACGGGAGCATCGATTACCAATTGGCAATGGATGCAGTGTGTGATAGAATTGGAGTGACGGAGGAATGTCCGGTAGAAAGCTTTCAAAATCAAGTTCTTCGAAAAGGATGTGAGAATCTTGGTTTGAAAGTCGACTCGGTTTCGAGAAATTCCTCAAAAGATCATTATTGTGGCTCTTGCTGTTATGGGTGTAGAGTTGGTGACAAGAAAGGGACTGATACTACTTGGCTGGTTGATGCTGTTGAATATGGTGCAGTAATCCTCACAGGCTGCCAAGCTGACAAATTCATTTTGGAGAACACAAATAACACAAGTAAAAGAAAAAGATGCATAGGAGTGATTGCAACTCCGCGAAGCAAAAACGTCACAAAAAAACTCCATATCGAGGCGAAAGCAACAATCTCTGCTTGTGGTTCTCTCCTGACACCCCCTCTACTTATTGCGAGCGGATTGCGTAATAAAAACATAGGTACCAATCTTCATTTGCATCCAGTTTCTTTGGTTTGGGGGTACTTCCCCGAAGAACTATCCGATTTTAAGGGTAAAATATATGAGGGAGGGCTCATTACTTCCCTGCACAAGGTAGTCTCAAAATATTCCAATTGCCATGCCATAATTGAAACCCCTGCAGTTGGACCGGCCTCATTTGCTGCACTATTTCCATGGACTTCCGGACGCGAAATGAAAGATGCAATGGTGAAGTACTCTCGAACTGCTCACCTTTTCGCTTTGATAAGAGACCAAGGTGCAGGAGAAATAAAGGTCAAGGCCAATGTCAAGTACCGACTCAACCAAACCGATAAAGAAAATCTAAGAATCGGTTTGAGGCAAGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTTGGCACCTACAGAAGTGATGGCCAAAGAATCAGATGTCAAGGTACAAACAAGGAAGAATTGGAGGAGTTCCTTGACTCAGTTGAGGCAGTTGGGGGACCATTGTCGAGGCAAAAGTACTGGACCATGTACTGCTCAGCACATCAGATGAGCAGCTGCAGAATGGGAAGCACAGAGGAAGAAGGAGCAGTGGATGAAAATGGTGAAAGTTGGGAAGCAGAAGGTTTATTTGTATGTGATGGAAGTGTGTTACCAACAGCAATTGGAGTGAATCCAATGATCACAATTCAATCCACAGCCTATTGCATCTCAAAGAAAATTGCAGAATCTTTATGA

mRNA sequence

ATGGAGGATACAGAAGTTCCATCCCACCCTTTATTGAGAAGACGAAGAACAGACCTCAACCATGACTTCTCTTCTTCTCAAATGGAAGCCATGGCTGCCTTCTGTGAAGCCCTCATTCCCCCTGTGCCTCTTTTTAAAGAAACCCCACTTGATCAATCTCTTCTTTCATTCTACCAATCCTCTGCTTTTCAAGCTCCAATCCCAGATGAGGTAGCTGACCTATTGGTGGAGAGGGCTAATCCAAAAGCTGTGTTTCTTGTGAAATTGGTTCTGAGACTTCTCTCTTTTAGAATTGGGACATTGTTGCTTTGCGGGAATGTTTGTTTTGATTGGAGATGGCCTTTCTTTCTTAAATTTTCTGAGATTTCTTTGGAGAAGAGAGAACGAATTCTCAAGAATTGGTCTATGGCTCACCACAAGTATACTGTACTTTTGAGAATGACTTTCATGATTATTAAAATTTTCTGCTGCTTCAAATTCTTCTCCAGGATCGATGAGGGAACGAAAAACGTTGCATGGAAAGGGATGGGGTATAAAGTAGACACCCGAAAACAGTTGAACCATACCCAAAAAGAGCGGCCTCTTGAAAGGGGAATGATAGAAACTGGCAATGAAGATGATTCCACTCTTCTCCGGTCTCTTTCACAGAAAGGCCTTGAAGTAATTGAAGATAAAAAACATGGTAATGCCTATAAGATCAAATGTGATCTTGTGATTGTTGGCTCGGGTTGTGGTGGTGGAGTGGCTGCTGCGGTTCTTGCAAAATCGGGTTTGAAGGTAGTCGTTCTCGAGAAAGGGAACTATTTCGTGGCTGAAGATTATTCTTCCCTAGAAGGTCCATCCATGAATGAACTATATGAGTCAGGTGGACTCAAGTCAACTCTCGATGGGAAGGTTATGCTGTTGGCTGGAACTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCAATTAGAACTCCAAAATCCATTCTCGATGAATGGTCTACTGATTATAAAATTCCTTTGTACGGGAGCATCGATTACCAATTGGCAATGGATGCAGTGTGTGATAGAATTGGAGTGACGGAGGAATGTCCGGTAGAAAGCTTTCAAAATCAAGTTCTTCGAAAAGGATGTGAGAATCTTGGTTTGAAAGTCGACTCGGTTTCGAGAAATTCCTCAAAAGATCATTATTGTGGCTCTTGCTGTTATGGGTGTAGAGTTGGTGACAAGAAAGGGACTGATACTACTTGGCTGGTTGATGCTGTTGAATATGGTGCAGTAATCCTCACAGGCTGCCAAGCTGACAAATTCATTTTGGAGAACACAAATAACACAAGTAAAAGAAAAAGATGCATAGGAGTGATTGCAACTCCGCGAAGCAAAAACGTCACAAAAAAACTCCATATCGAGGCGAAAGCAACAATCTCTGCTTGTGGTTCTCTCCTGACACCCCCTCTACTTATTGCGAGCGGATTGCGTAATAAAAACATAGGTACCAATCTTCATTTGCATCCAGTTTCTTTGGTTTGGGGGTACTTCCCCGAAGAACTATCCGATTTTAAGGGTAAAATATATGAGGGAGGGCTCATTACTTCCCTGCACAAGGTAGTCTCAAAATATTCCAATTGCCATGCCATAATTGAAACCCCTGCAGTTGGACCGGCCTCATTTGCTGCACTATTTCCATGGACTTCCGGACGCGAAATGAAAGATGCAATGGTGAAGTACTCTCGAACTGCTCACCTTTTCGCTTTGATAAGAGACCAAGGTGCAGGAGAAATAAAGGTCAAGGCCAATGTCAAGTACCGACTCAACCAAACCGATAAAGAAAATCTAAGAATCGGTTTGAGGCAAGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTTGGCACCTACAGAAGTGATGGCCAAAGAATCAGATGTCAAGGTACAAACAAGGAAGAATTGGAGGAGTTCCTTGACTCAGTTGAGGCAGTTGGGGGACCATTGTCGAGGCAAAAGTACTGGACCATGTACTGCTCAGCACATCAGATGAGCAGCTGCAGAATGGGAAGCACAGAGGAAGAAGGAGCAGTGGATGAAAATGGTGAAAGTTGGGAAGCAGAAGGTTTATTTGTATGTGATGGAAGTGTGTTACCAACAGCAATTGGAGTGAATCCAATGATCACAATTCAATCCACAGCCTATTGCATCTCAAAGAAAATTGCAGAATCTTTATGA

Coding sequence (CDS)

ATGGAGGATACAGAAGTTCCATCCCACCCTTTATTGAGAAGACGAAGAACAGACCTCAACCATGACTTCTCTTCTTCTCAAATGGAAGCCATGGCTGCCTTCTGTGAAGCCCTCATTCCCCCTGTGCCTCTTTTTAAAGAAACCCCACTTGATCAATCTCTTCTTTCATTCTACCAATCCTCTGCTTTTCAAGCTCCAATCCCAGATGAGGTAGCTGACCTATTGGTGGAGAGGGCTAATCCAAAAGCTGTGTTTCTTGTGAAATTGGTTCTGAGACTTCTCTCTTTTAGAATTGGGACATTGTTGCTTTGCGGGAATGTTTGTTTTGATTGGAGATGGCCTTTCTTTCTTAAATTTTCTGAGATTTCTTTGGAGAAGAGAGAACGAATTCTCAAGAATTGGTCTATGGCTCACCACAAGTATACTGTACTTTTGAGAATGACTTTCATGATTATTAAAATTTTCTGCTGCTTCAAATTCTTCTCCAGGATCGATGAGGGAACGAAAAACGTTGCATGGAAAGGGATGGGGTATAAAGTAGACACCCGAAAACAGTTGAACCATACCCAAAAAGAGCGGCCTCTTGAAAGGGGAATGATAGAAACTGGCAATGAAGATGATTCCACTCTTCTCCGGTCTCTTTCACAGAAAGGCCTTGAAGTAATTGAAGATAAAAAACATGGTAATGCCTATAAGATCAAATGTGATCTTGTGATTGTTGGCTCGGGTTGTGGTGGTGGAGTGGCTGCTGCGGTTCTTGCAAAATCGGGTTTGAAGGTAGTCGTTCTCGAGAAAGGGAACTATTTCGTGGCTGAAGATTATTCTTCCCTAGAAGGTCCATCCATGAATGAACTATATGAGTCAGGTGGACTCAAGTCAACTCTCGATGGGAAGGTTATGCTGTTGGCTGGAACTACTGTGGGTGGAGGCTCTGCTATAAACTGGTCTGCATCAATTAGAACTCCAAAATCCATTCTCGATGAATGGTCTACTGATTATAAAATTCCTTTGTACGGGAGCATCGATTACCAATTGGCAATGGATGCAGTGTGTGATAGAATTGGAGTGACGGAGGAATGTCCGGTAGAAAGCTTTCAAAATCAAGTTCTTCGAAAAGGATGTGAGAATCTTGGTTTGAAAGTCGACTCGGTTTCGAGAAATTCCTCAAAAGATCATTATTGTGGCTCTTGCTGTTATGGGTGTAGAGTTGGTGACAAGAAAGGGACTGATACTACTTGGCTGGTTGATGCTGTTGAATATGGTGCAGTAATCCTCACAGGCTGCCAAGCTGACAAATTCATTTTGGAGAACACAAATAACACAAGTAAAAGAAAAAGATGCATAGGAGTGATTGCAACTCCGCGAAGCAAAAACGTCACAAAAAAACTCCATATCGAGGCGAAAGCAACAATCTCTGCTTGTGGTTCTCTCCTGACACCCCCTCTACTTATTGCGAGCGGATTGCGTAATAAAAACATAGGTACCAATCTTCATTTGCATCCAGTTTCTTTGGTTTGGGGGTACTTCCCCGAAGAACTATCCGATTTTAAGGGTAAAATATATGAGGGAGGGCTCATTACTTCCCTGCACAAGGTAGTCTCAAAATATTCCAATTGCCATGCCATAATTGAAACCCCTGCAGTTGGACCGGCCTCATTTGCTGCACTATTTCCATGGACTTCCGGACGCGAAATGAAAGATGCAATGGTGAAGTACTCTCGAACTGCTCACCTTTTCGCTTTGATAAGAGACCAAGGTGCAGGAGAAATAAAGGTCAAGGCCAATGTCAAGTACCGACTCAACCAAACCGATAAAGAAAATCTAAGAATCGGTTTGAGGCAAGCATTGAGAATCTTGATTGCAGCAGGAGCAACTGAGGTTGGCACCTACAGAAGTGATGGCCAAAGAATCAGATGTCAAGGTACAAACAAGGAAGAATTGGAGGAGTTCCTTGACTCAGTTGAGGCAGTTGGGGGACCATTGTCGAGGCAAAAGTACTGGACCATGTACTGCTCAGCACATCAGATGAGCAGCTGCAGAATGGGAAGCACAGAGGAAGAAGGAGCAGTGGATGAAAATGGTGAAAGTTGGGAAGCAGAAGGTTTATTTGTATGTGATGGAAGTGTGTTACCAACAGCAATTGGAGTGAATCCAATGATCACAATTCAATCCACAGCCTATTGCATCTCAAAGAAAATTGCAGAATCTTTATGA

Protein sequence

MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQSSAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEYGAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMITIQSTAYCISKKIAESL*
Homology
BLAST of Chy4G078160 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 2.7e-273
Identity = 475/744 (63.84%), Postives = 583/744 (78.36%), Query Frame = 0

Query: 8   SHPLL---RRRRTDLNHDFSSSQMEAMAAFCEALIPPVPL---FKETPLDQSLLSFYQSS 67
           +HPLL   RR+    +H  SSSQM  +AA CEAL P  PL     ++ +D++L +FY +S
Sbjct: 9   THPLLKGGRRKEKGYSHGLSSSQMHVIAAICEALFPSQPLDSQNNQSSVDKALSAFYTAS 68

Query: 68  AFQAPIPDEVADLLVE--RANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKF 127
             QAP+PDE A+LL +  R+ P+A+ LV  VL +LSFR+GTLLLCG +C DWRWPF  KF
Sbjct: 69  GSQAPLPDEAAELLFKFNRSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWRWPFIHKF 128

Query: 128 SEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYK 187
           SEI LEKRE ILK WS    K  + LR+ F++ K+ C +  FSR D    N  WK +GY+
Sbjct: 129 SEIPLEKREEILKRWS--REKCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKAIGYQ 188

Query: 188 VDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVI 247
           VDTR++L  TQK+RPL+ G+IET  E DSTL++SL++KGLEV ED +  N YKIKCD VI
Sbjct: 189 VDTREKL--TQKKRPLQEGLIETMYETDSTLIQSLTEKGLEVTEDLEQ-NMYKIKCDAVI 248

Query: 248 VGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKV 307
           VGSGCGGGVAAAVLA SG KV++LEKG YFV+ DYSSLEGPSMNELYESGG+  +LDGK+
Sbjct: 249 VGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESGGILPSLDGKM 308

Query: 308 MLLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEE 367
           M+LAG+T+GG SAINWSA IRTP S+L EWS  +KIPL+ S DYQ AMD VC RIGVTE 
Sbjct: 309 MILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDTVCRRIGVTEN 368

Query: 368 CPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVE 427
           C  ESFQNQ+LR+GC  +G KV+ V+ NSS DHYCGSCCYGCR GDKKGT++TWLVDAV 
Sbjct: 369 CNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGTESTWLVDAVG 428

Query: 428 YGAVILTGCQADK--FILENTNNTSKRKRCIGVI--ATPRSKNVTKKLHIEAKATISACG 487
            GAVILTGC+A+K  F L++ +N +KRK C GVI  AT RSK VTKKL IE+K TISACG
Sbjct: 429 NGAVILTGCKAEKLNFTLKDGDNGTKRKTCSGVIASATWRSK-VTKKLQIESKVTISACG 488

Query: 488 SLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK 547
           SL TPPL+I+SGL+N NIG NLHLHP    WGYFPE++++F G  YEGG+ITS+HKV  +
Sbjct: 489 SLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNFSGNNYEGGIITSIHKVFEE 548

Query: 548 YSNC--HAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVK 607
            S      IIE PA+GP SF+AL PW SG ++K+ MVKY+RTA+LFAL+RD G+GE+K +
Sbjct: 549 DSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYARTANLFALVRDHGSGEVKAE 608

Query: 608 ANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDS 667
             + Y+L++ D+E+L+ GLR+ALRIL+AAGA EVGTYRSDGQRI+C+G  + +LEEFLDS
Sbjct: 609 GRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRSDGQRIKCRGIKESDLEEFLDS 668

Query: 668 VEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTA 727
           V  VGGP SR + WT++ SAHQM+SCRM +TEEEGAVDENGESWEA+GL+VCDGSVLP+A
Sbjct: 669 VRVVGGPSSRNEVWTVFTSAHQMTSCRMSATEEEGAVDENGESWEAKGLYVCDGSVLPSA 728

Query: 728 IGVNPMITIQSTAYCISKKIAESL 738
           +GVNPMITIQSTAYCI+  IAESL
Sbjct: 729 VGVNPMITIQSTAYCIASNIAESL 746

BLAST of Chy4G078160 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 867.1 bits (2239), Expect = 1.5e-250
Identity = 430/738 (58.27%), Postives = 553/738 (74.93%), Query Frame = 0

Query: 9   HPLLR--RRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQSSAFQAP 68
           HPLLR  +R    NH FS SQ++++A  C+  +PP    + T   Q++ SF+ +S+ Q P
Sbjct: 11  HPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPP----ETTSEQQAVNSFHVASSTQPP 70

Query: 69  IPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFSEISLEK 128
             DEVA+++V+    +AV +++++L +LSFR GTLLLCG++C D  WPF LKFS++ L+K
Sbjct: 71  FTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDK 130

Query: 129 RERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKVD----- 188
           RE IL+NWS     + +  R+TF + K +  F FFS+ DE  KN A + +GY +D     
Sbjct: 131 REAILRNWS-RQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTERS 190

Query: 189 TRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIVG 248
           + K+    +K RPLE+G+IET +E D T+ +SL++KG+ V  D    N Y+I+CD V+VG
Sbjct: 191 SNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVARD-DGDNVYRIRCDAVVVG 250

Query: 249 SGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVML 308
           SG GGGVAAA LAK+GLKV+VLEKGNYF A DYS LE PSM ELYE GGL +T+DGK ML
Sbjct: 251 SGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVDGKFML 310

Query: 309 LAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECP 368
           LAG+ VGGG+A+NWSASIRTP  +L EWS   KI  +GS +YQ AMD V  RIGVTE C 
Sbjct: 311 LAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGVTERCV 370

Query: 369 VESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEYG 428
              FQNQVLRKGCE LGL+V+SV RNS +DHYCG C YGCR G K GTD TWLVDAVE G
Sbjct: 371 KHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVDAVENG 430

Query: 429 AVILTGCQADKFIL-ENTNNTSKR-KRCIGVIATPRSKNVTKKLHIEAKATISACGSLLT 488
           AVILTG +A++F+L +NT+++++R KRC+GV A+     + KK  IEA+ T+S+ GSLLT
Sbjct: 431 AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSSAGSLLT 490

Query: 489 PPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNC 548
           PPL+++SGL+N NIG NL LHPV + WGYFPE+ S+F GK+YEGG+ITS+H +    S C
Sbjct: 491 PPLMLSSGLKNPNIGRNLKLHPVLMTWGYFPEKDSEFSGKMYEGGIITSVHHMNDTESGC 550

Query: 549 HAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYR 608
            AI+E P +GPAS+A L PW SG ++K+ M+KY RTAHLFAL+RD G+GE+ ++  V YR
Sbjct: 551 KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDLGSGEVMMENEVTYR 610

Query: 609 LNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGG 668
             + D+ENLR GLRQALR+ +AAGA EVGTYRSDGQ+++C+   KE +EEFLD V+AVGG
Sbjct: 611 TTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGG 670

Query: 669 PLSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPM 728
             ++ +YWT Y SAHQM SCRMG T EEGA+DENGESWEAEGLFVCDGS+LP+A+GVNPM
Sbjct: 671 VGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVCDGSILPSAVGVNPM 730

Query: 729 ITIQSTAYCISKKIAESL 738
           ITIQSTAYCIS KI +SL
Sbjct: 731 ITIQSTAYCISSKIVDSL 742

BLAST of Chy4G078160 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 7.0e-245
Identity = 416/742 (56.06%), Postives = 558/742 (75.20%), Query Frame = 0

Query: 9   HPLLRRRRTD--LNHDFSSSQMEAMAAFCEALIPPVPL--FKETPLD----QSLLSFYQS 68
           HPLLR  R D      FSSS+ME++A+ CE ++PP+P+   K    D    + +  F+ +
Sbjct: 6   HPLLRGGRKDNKFRQGFSSSEMESLASICEVILPPLPMDALKIKKHDDVSIEDVEFFWNT 65

Query: 69  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 128
           SA   PIP EVA++L +R+  +AV L+++VL LL+ R+GT LLCG +CF  +WPF   FS
Sbjct: 66  SASHYPIPHEVAEILSKRSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKWPFINNFS 125

Query: 129 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 188
            +SL+KRER+++ W    +++   +R+ F  +K+ C F +F+ +DE   N AWK +GY+V
Sbjct: 126 SMSLKKRERVMQWW--LKNRFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKAIGYEV 185

Query: 189 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 248
              ++L ++   R LE+G++ET +E +STL +SL++KGL V  D ++ N  K+KCD ++V
Sbjct: 186 SADEKLPNSSNGRLLEKGIVETMHETNSTLQQSLTEKGLNVTLDSRN-NILKVKCDAIVV 245

Query: 249 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 308
           GSGCGGGVAA+VL+ +G KVVVLEKGNYF   DYSSLEGPSM++LYE+GG+ +++D +++
Sbjct: 246 GSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLYETGGILASVDSRIL 305

Query: 309 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 368
           LLAG+TVGGGSA+NWSA I+TP  +L EWS ++ + L+ + +Y  AM+ VC+RIGVTE C
Sbjct: 306 LLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSAMETVCERIGVTESC 365

Query: 369 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 428
             E FQNQVLRKGC+NLGLKVD V RNSS +H+CGSC YGC  G+K+GT  TWLVDAVE 
Sbjct: 366 THEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEKQGTQDTWLVDAVEN 425

Query: 429 GAVILTGCQADKFILENTNNTS-KRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLT 488
           GAVI+TGC+A++F+LEN  N S ++K+C+GV+A   S  VT KL IEAK TISA G+LLT
Sbjct: 426 GAVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQIEAKVTISAGGALLT 485

Query: 489 PPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSK---- 548
           PPL+I+SGL+NKNIG NLHLHPV + WGYFPE  S+ KGKI+EGG+ITS+HKV S+    
Sbjct: 486 PPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTSELKGKIFEGGIITSVHKVPSRDSNS 545

Query: 549 YSNCHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKAN 608
           YS+  AIIETP++GP SFA+L PW SG + K+ M+ Y RTAHL  +IRD+  G++  +  
Sbjct: 546 YSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTAHLITIIRDKACGQVTTEGR 605

Query: 609 VKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVE 668
           V Y+L+  DKEN++ GL+QALRIL AAGA EVGT+RSDGQR++C   ++ E+ EF+DSV 
Sbjct: 606 VSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQRVKCGEVSENEMAEFIDSVC 665

Query: 669 AVGGPLSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIG 728
            + G LS  + W +Y SAHQM SCRMG  E+EGAVDENGESWEA+GLFVCD S+LPTA+G
Sbjct: 666 PMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENGESWEAQGLFVCDASLLPTAVG 725

Query: 729 VNPMITIQSTAYCISKKIAESL 738
           VNPMITIQSTAYCIS ++ + L
Sbjct: 726 VNPMITIQSTAYCISNRVVDYL 744

BLAST of Chy4G078160 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 1.1e-234
Identity = 404/734 (55.04%), Postives = 541/734 (73.71%), Query Frame = 0

Query: 22  DFSSSQMEAMAAFCEALIPPV-PLFKETPLD-------QSLLSFYQSSAFQAPIPDEVAD 81
           D + ++ME++A+FCEA++P V P  +E   +       ++L SFY +S  + P+  +  +
Sbjct: 14  DITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIE 73

Query: 82  LLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFSEISLEKRERIL-- 141
           L+ +R   +A    +L+L LL+ R+GTLL+CG  C   RWPF  KFSE+SLEKRER+L  
Sbjct: 74  LVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQK 133

Query: 142 --KNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKVD-TRKQLNH 201
             KNW +        +R  F+ IK+   F FFSR++   +N AW+ +GY+V+    + + 
Sbjct: 134 QFKNWILTP------IRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSE 193

Query: 202 TQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIVGSGCGGGV 261
           T  ERPLE+G++ET  E + TLL SL+ KGLE + D +H +A +IKCD+V+VGSG GGGV
Sbjct: 194 THNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDTEH-DAIRIKCDVVVVGSGSGGGV 253

Query: 262 AAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVG 321
           AA+VLAKSGLKVVVLEKG+YF   ++   EGP +++LYE+GG+  ++DG  M+LAG TVG
Sbjct: 254 AASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATVG 313

Query: 322 GGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECPVESFQNQ 381
           GGSA+NWSA I+TPKS+L EWS D  IPL+G+ +Y  AM+ V  R+GVTE+C +ESFQNQ
Sbjct: 314 GGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQNQ 373

Query: 382 VLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEYGAVILTGC 441
           +LRKGCENLG  V++V RNSS+ HYCGSC YGCR GDKKG+D TWLVDAV +GAVILTGC
Sbjct: 374 ILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTGC 433

Query: 442 QADKFILE--NTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTPPLLIAS 501
           +A++FILE   +N   K+ +C+GV+A   + N+ K L IEAK T+SA G+LLTPPL+I+S
Sbjct: 434 KAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMISS 493

Query: 502 GLRNKNIGTNLHLHPVSLVWGYFPEELS---DFKGKIYEGGLITSLHKVVSKYSNCHAII 561
           GLRN+NIG NLHLHPV + WGYFP++ S    FKG  YEGG+ITS+ KV+S+ S   AII
Sbjct: 494 GLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSEVRAII 553

Query: 562 ETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRLNQT 621
           ETP +GP SF+ L PWTSG +MK  M +YSRTA L  ++RD+G+GE+K +  + Y +++T
Sbjct: 554 ETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTVDKT 613

Query: 622 DKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGPLSR 681
           D++NL+ GLR++LRILIAAGA EVGT+RSDGQR+ C+G N+  ++EFLDSV    G    
Sbjct: 614 DRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLICKGVNENSIQEFLDSVSTEEGAKGM 673

Query: 682 QKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMITIQ 738
            + W +Y SAHQM SCR+G  E+EGA+D NGESWEAE LFVCD S LP+A+GVNPMIT+ 
Sbjct: 674 TEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWEAEKLFVCDASALPSAVGVNPMITVM 733

BLAST of Chy4G078160 vs. ExPASy Swiss-Prot
Match: Q9ZWB9 (Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=2)

HSP 1 Score: 806.2 bits (2081), Expect = 3.1e-232
Identity = 407/743 (54.78%), Postives = 540/743 (72.68%), Query Frame = 0

Query: 10  PLLR--RRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFK-------ETPLDQSLLSFYQS 69
           PLLR   ++   +H FS S ++A+++ C+A++PPVPL         +   + +LLSF++S
Sbjct: 13  PLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLRNDALLSFFKS 72

Query: 70  SAFQAPI-PDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWR-WPFFLK 129
           S+ ++ + PDEVA+LL  +A P  V +V++VLR+L+FR+GTLLLCG VC D + WPF LK
Sbjct: 73  SSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFLLK 132

Query: 130 FSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGY 189
           FSE+SLEKRE++L+ W+     Y  L R+ FM+IK    F +F+  +E ++N  W  + Y
Sbjct: 133 FSEMSLEKREKVLQRWNT--QWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAINY 192

Query: 190 KVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLV 249
            V+  +  +  QKERPL+ G+IET  ED+ T+ + +  KGL+V ED++  + YKI+CD V
Sbjct: 193 SVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRER-DTYKIECDAV 252

Query: 250 IVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGK 309
           +VGSGCGGGVAAA+LAKSGL+VVV+EKGNYF   DYS+LEGPSM EL+ES  L  T DG+
Sbjct: 253 VVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFELFESNSLMMTHDGR 312

Query: 310 VMLLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTE 369
              +AG+TVGGGS +NW+AS++TP +I++EWS    I +Y S  Y+ AM  VC R+GVTE
Sbjct: 313 FRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYKAAMGIVCKRLGVTE 372

Query: 370 ECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAV 429
           +   E FQNQ+LRKGCE LGL V  V RNS++ HYCGSC YGC  G+K+GTD+TWLVDAV
Sbjct: 373 KIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAV 432

Query: 430 EYGAVILTGCQADKFILENTN-----NTSKRKRCIGVIATPRSKNVTKKLHIEAKATISA 489
              AVILT C+A+K IL + +      + +RKRC+GV A+  S    KKL I AK TI A
Sbjct: 433 NNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAAS-LSHQTRKKLQINAKVTIVA 492

Query: 490 CGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVV 549
           CGSL TP LL +SGL+N NI   LH+HP+ + WGYFPE+ S+ +G  +EG ++TSLH V 
Sbjct: 493 CGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNSELEGAAHEGEIVTSLHYVH 552

Query: 550 SKYSNCHAI-IETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKV 609
              S    I +ETPA+GP +FAAL PW SG +MK+ M KY+RTAH+FA++RD+G GE+K 
Sbjct: 553 PMDSTTPNITLETPAIGPGTFAALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVKG 612

Query: 610 KANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLD 669
              VKYRL + D+ENL IGL+QALRIL+AAGA EVGTYRSDGQR++C G  +++LE FLD
Sbjct: 613 DI-VKYRLTKADEENLTIGLKQALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLD 672

Query: 670 SVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPT 729
           +V A  G +S  K+WT   +AHQ+  CRMG+TE+EGA+D  GESWEAE L+VCD SVLPT
Sbjct: 673 TVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVLPT 732

Query: 730 AIGVNPMITIQSTAYCISKKIAE 736
           A+GVNPMIT+QSTAYCIS +IAE
Sbjct: 733 ALGVNPMITVQSTAYCISNRIAE 750

BLAST of Chy4G078160 vs. ExPASy TrEMBL
Match: A0A0A0LBV7 (Long-chain-alcohol oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G575330 PE=3 SV=1)

HSP 1 Score: 1472.6 bits (3811), Expect = 0.0e+00
Identity = 728/737 (98.78%), Postives = 735/737 (99.73%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MEDTEVPSHPLLR RRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKE+PLDQSLLSFYQS
Sbjct: 1   MEDTEVPSHPLLRTRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKESPLDQSLLSFYQS 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS
Sbjct: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV
Sbjct: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCD+VIV
Sbjct: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASI+TPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC
Sbjct: 301 LLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
           PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY
Sbjct: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGCQADKFIL+NTNNTSKRKRCIGVIATPRSKN+TKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSN H
Sbjct: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL
Sbjct: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP
Sbjct: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
           LSRQKYWTMYCSAHQMSSCRMGST+EEGAVDENGESWEAEGLFVCDGSVLPTA+GVNPMI
Sbjct: 661 LSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 738
           TIQSTAYCISKKIAESL
Sbjct: 721 TIQSTAYCISKKIAESL 737

BLAST of Chy4G078160 vs. ExPASy TrEMBL
Match: A0A5A7T3R3 (Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001660 PE=3 SV=1)

HSP 1 Score: 1426.0 bits (3690), Expect = 0.0e+00
Identity = 704/737 (95.52%), Postives = 720/737 (97.69%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MEDTE  SHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPP+PLF ETPLDQ LLSFYQS
Sbjct: 1   MEDTESTSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPLPLFSETPLDQFLLSFYQS 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS
Sbjct: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK MGY+V
Sbjct: 121 EISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKAMGYEV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKCD+VIV
Sbjct: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIGVT  C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIGVTRGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLVDAV+ 
Sbjct: 361 RVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLVDAVKC 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGC ADKFILENTNNTSK+K+CIGVIATP SKNVTKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCHADKFILENTNNTSKKKKCIGVIATPLSKNVTKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSKYSN H
Sbjct: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSKYSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKANVKYRL
Sbjct: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           NQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVEAVGGP
Sbjct: 601 NQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVEAVGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
            SRQKYWTMYCSAHQMSSCRMGST+EEGAVDENGESWEAEGLFVCDGSVLPTA+GVNPMI
Sbjct: 661 WSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 738
           TIQSTAYCISKKIAESL
Sbjct: 721 TIQSTAYCISKKIAESL 737

BLAST of Chy4G078160 vs. ExPASy TrEMBL
Match: A0A1S3BHB1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103489850 PE=3 SV=1)

HSP 1 Score: 1423.7 bits (3684), Expect = 0.0e+00
Identity = 703/737 (95.39%), Postives = 719/737 (97.56%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MEDTE  SHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPP+PLF ETPLDQ LLSFYQS
Sbjct: 1   MEDTESTSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPLPLFSETPLDQFLLSFYQS 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS
Sbjct: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK MGY+V
Sbjct: 121 EISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKAMGYEV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKCD+VIV
Sbjct: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIGVT  C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIGVTRGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLVDAV+ 
Sbjct: 361 RVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLVDAVKC 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGC ADKFILENTNNTSK+ +CIGVIATP SKNVTKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCHADKFILENTNNTSKKXKCIGVIATPLSKNVTKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSKYSN H
Sbjct: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSKYSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKANVKYRL
Sbjct: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           NQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVEAVGGP
Sbjct: 601 NQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVEAVGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
            SRQKYWTMYCSAHQMSSCRMGST+EEGAVDENGESWEAEGLFVCDGSVLPTA+GVNPMI
Sbjct: 661 WSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 738
           TIQSTAYCISKKIAESL
Sbjct: 721 TIQSTAYCISKKIAESL 737

BLAST of Chy4G078160 vs. ExPASy TrEMBL
Match: A0A6J1HWN7 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466966 PE=3 SV=1)

HSP 1 Score: 1302.3 bits (3369), Expect = 0.0e+00
Identity = 637/737 (86.43%), Postives = 689/737 (93.49%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MED EV SHPLLRRRRT+L+H FSSSQMEAMAAFCEALIPP+PL KE PLDQSLL+FY++
Sbjct: 1   MEDREVRSHPLLRRRRTELSHGFSSSQMEAMAAFCEALIPPLPLVKEDPLDQSLLAFYEA 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SA QAPIPDEVA LLV+RANPKAVFLVKLVL LLSFRIGTLLLCG +CFDWRWPF LKFS
Sbjct: 61  SASQAPIPDEVAALLVDRANPKAVFLVKLVLMLLSFRIGTLLLCGYLCFDWRWPFVLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EIS EKRE+ILK+WS+AHH+YTVLLRM FMIIK+FCCFKFFSRID  +KN+AW+ MGY V
Sbjct: 121 EISPEKREQILKDWSVAHHRYTVLLRMVFMIIKMFCCFKFFSRIDRESKNIAWEAMGYHV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRK+LN ++KERPLERG+IETG EDDSTLLRSLS KGL V ED+KHG+ Y IKCD+VIV
Sbjct: 181 DTRKRLNDSRKERPLERGLIETGKEDDSTLLRSLSMKGLAVTEDQKHGSTYTIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFV EDYSSLEGPSMNELYE+GGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYETGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPK +L+EWST +KIPL+GS DYQ AMDAVCDRIGVTE C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKPVLEEWSTIHKIPLFGSFDYQSAMDAVCDRIGVTEGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            +ESFQNQVLRKGCENLGLKVDSV RNSSKDHYCGSCCYGCRVGDKKGTD+TWLVDAVE 
Sbjct: 361 RLESFQNQVLRKGCENLGLKVDSVPRNSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVES 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGC+ADK +LENTNNTS+ KRC+GVIATP SKNVTKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCRADKLVLENTNNTSRNKRCLGVIATPLSKNVTKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIG+NLHLHPV L WGYFPE+LSDF GKIYEGGLITSLH VVSK SN H
Sbjct: 481 PLLIASGLRNKNIGSNLHLHPVILAWGYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AII+TPA+GPASFAA+FPWTSGR+MKDA+VKY+RTAHLFAL+RD+GAGEIKVKANVKYRL
Sbjct: 541 AIIQTPALGPASFAAMFPWTSGRDMKDAVVKYARTAHLFALVRDRGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           +QTDKENLRIGLRQALRILIAAGATEVGTYRSDGQR +  GTNKE+LEEF DSVEA GGP
Sbjct: 601 DQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRFQFHGTNKEDLEEFFDSVEAAGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
            SR+KYW +YCSAHQMSSCRMGSTEEEGAVDENGESWEA+GLFVCDGSVLPTA+GVNPMI
Sbjct: 661 FSREKYWNIYCSAHQMSSCRMGSTEEEGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 738
           TIQSTAYCISKK+AESL
Sbjct: 721 TIQSTAYCISKKLAESL 737

BLAST of Chy4G078160 vs. ExPASy TrEMBL
Match: A0A6J1HG83 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111464000 PE=3 SV=1)

HSP 1 Score: 1288.9 bits (3334), Expect = 0.0e+00
Identity = 631/737 (85.62%), Postives = 685/737 (92.94%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MED EV SHPLLRRRRT+L+H FSSSQMEAMAAFCEALIPP+PL KE PL QS+L+FY++
Sbjct: 1   MEDREVRSHPLLRRRRTELSHGFSSSQMEAMAAFCEALIPPLPLVKEDPLHQSILAFYEA 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SA QAPIPDEVA LLV+RANPKAVFLVKLVLRLLSFRIGTLLLCG +CFDWRWPF LKFS
Sbjct: 61  SASQAPIPDEVAALLVDRANPKAVFLVKLVLRLLSFRIGTLLLCGYLCFDWRWPFVLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EISLEKRE+ILK+WS+AH +YTVLLRM FMIIK+FCCFKFFS ID  +KN+AW+ MGY+V
Sbjct: 121 EISLEKREQILKDWSVAHQRYTVLLRMVFMIIKMFCCFKFFSGIDRESKNIAWEAMGYRV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRK+LN T+KERPLERG+IE G EDDSTLL+SLS KGL V ED KHG+ Y IKCD+VIV
Sbjct: 181 DTRKRLNDTRKERPLERGVIEAGKEDDSTLLQSLSMKGLAVTEDPKHGSTYTIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFV EDYSSLEGPSMNELYE+GGLKST+DGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYETGGLKSTIDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPK +L+EWST +KIPL+GS DY+ AMDAVCDRIGVTE C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKPVLEEWSTIHKIPLFGSFDYRSAMDAVCDRIGVTEGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            +ESFQNQVLRKGCENLGLKVDSV RNSSKDHYCGSCCYGCRVGDKKGTD TWLVDAVE 
Sbjct: 361 RLESFQNQVLRKGCENLGLKVDSVPRNSSKDHYCGSCCYGCRVGDKKGTDATWLVDAVES 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGC+ADK +LENTNNTS+ KRC+GVIAT  SKNVTKKL+IEAKATISACGSLLTP
Sbjct: 421 GAVILTGCRADKLVLENTNNTSRNKRCLGVIATMLSKNVTKKLYIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIG+NLHLHPV L WGYFPE+LSDF GKIYEGGLITSLH VVSK SN H
Sbjct: 481 PLLIASGLRNKNIGSNLHLHPVILAWGYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AII+TPA+GPASFAA+FPWTSGR+MKDAMVKY+RTAHLFAL+RD+GAGEIK KANVKYRL
Sbjct: 541 AIIQTPALGPASFAAMFPWTSGRDMKDAMVKYARTAHLFALVRDKGAGEIKAKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           +QTDKENLRIGLRQALRILIAAGATEVGTYRS+GQR R  GTNKE+LEEFLDSVEA GGP
Sbjct: 601 DQTDKENLRIGLRQALRILIAAGATEVGTYRSNGQRFRFHGTNKEDLEEFLDSVEAAGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
            SR+KYW +YCSAHQMSSCRMGSTEEEGAVDENGESWEA+GLFVCDGSVLPTA+GVNPMI
Sbjct: 661 FSREKYWNIYCSAHQMSSCRMGSTEEEGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 738
           TIQSTAYCISKK+AESL
Sbjct: 721 TIQSTAYCISKKLAESL 737

BLAST of Chy4G078160 vs. NCBI nr
Match: XP_004152057.1 (long-chain-alcohol oxidase FAO2 [Cucumis sativus] >KGN58167.1 hypothetical protein Csa_017539 [Cucumis sativus])

HSP 1 Score: 1470 bits (3805), Expect = 0.0
Identity = 728/737 (98.78%), Postives = 735/737 (99.73%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MEDTEVPSHPLLR RRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKE+PLDQSLLSFYQS
Sbjct: 1   MEDTEVPSHPLLRTRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKESPLDQSLLSFYQS 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS
Sbjct: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV
Sbjct: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCD+VIV
Sbjct: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASI+TPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC
Sbjct: 301 LLAGTTVGGGSAINWSASIKTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
           PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY
Sbjct: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGCQADKFIL+NTNNTSKRKRCIGVIATPRSKN+TKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCQADKFILDNTNNTSKRKRCIGVIATPRSKNITKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSN H
Sbjct: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL
Sbjct: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP
Sbjct: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
           LSRQKYWTMYCSAHQMSSCRMGST+EEGAVDENGESWEAEGLFVCDGSVLPTA+GVNPMI
Sbjct: 661 LSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 737
           TIQSTAYCISKKIAESL
Sbjct: 721 TIQSTAYCISKKIAESL 737

BLAST of Chy4G078160 vs. NCBI nr
Match: KAA0037980.1 (long-chain-alcohol oxidase FAO2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1423 bits (3684), Expect = 0.0
Identity = 704/737 (95.52%), Postives = 720/737 (97.69%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MEDTE  SHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPP+PLF ETPLDQ LLSFYQS
Sbjct: 1   MEDTESTSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPLPLFSETPLDQFLLSFYQS 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS
Sbjct: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK MGY+V
Sbjct: 121 EISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKAMGYEV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKCD+VIV
Sbjct: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIGVT  C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIGVTRGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLVDAV+ 
Sbjct: 361 RVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLVDAVKC 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGC ADKFILENTNNTSK+K+CIGVIATP SKNVTKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCHADKFILENTNNTSKKKKCIGVIATPLSKNVTKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSKYSN H
Sbjct: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSKYSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKANVKYRL
Sbjct: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           NQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVEAVGGP
Sbjct: 601 NQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVEAVGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
            SRQKYWTMYCSAHQMSSCRMGST+EEGAVDENGESWEAEGLFVCDGSVLPTA+GVNPMI
Sbjct: 661 WSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 737
           TIQSTAYCISKKIAESL
Sbjct: 721 TIQSTAYCISKKIAESL 737

BLAST of Chy4G078160 vs. NCBI nr
Match: XP_008447391.2 (PREDICTED: LOW QUALITY PROTEIN: long-chain-alcohol oxidase FAO2-like [Cucumis melo])

HSP 1 Score: 1421 bits (3678), Expect = 0.0
Identity = 703/737 (95.39%), Postives = 719/737 (97.56%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MEDTE  SHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPP+PLF ETPLDQ LLSFYQS
Sbjct: 1   MEDTESTSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPLPLFSETPLDQFLLSFYQS 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS
Sbjct: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EISLEKRE+ILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWK MGY+V
Sbjct: 121 EISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKAMGYEV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRKQLNHTQKERPLERGMIETGNEDDSTL RSLSQKGLEVIEDKKHGNAYKIKCD+VIV
Sbjct: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPKS+LDEWSTD+KIPLYGSIDYQLAMDAVCDRIGVT  C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIGVTRGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            VESFQNQVLRKGCENLGLKVDSVSRNSS+DHYCGSCCYGCRVGDKKGTDTTWLVDAV+ 
Sbjct: 361 RVESFQNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLVDAVKC 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGC ADKFILENTNNTSK+ +CIGVIATP SKNVTKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCHADKFILENTNNTSKKXKCIGVIATPLSKNVTKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLIT LH VVSKYSN H
Sbjct: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSKYSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFAL+RDQGAGEIKVKANVKYRL
Sbjct: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           NQTDKENL+IGLRQALRILIAAGATE+GTYRSDGQRIRCQGT KE+LEEFLDSVEAVGGP
Sbjct: 601 NQTDKENLKIGLRQALRILIAAGATEIGTYRSDGQRIRCQGTKKEDLEEFLDSVEAVGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
            SRQKYWTMYCSAHQMSSCRMGST+EEGAVDENGESWEAEGLFVCDGSVLPTA+GVNPMI
Sbjct: 661 WSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 737
           TIQSTAYCISKKIAESL
Sbjct: 721 TIQSTAYCISKKIAESL 737

BLAST of Chy4G078160 vs. NCBI nr
Match: XP_038888257.1 (long-chain-alcohol oxidase FAO2-like [Benincasa hispida])

HSP 1 Score: 1351 bits (3496), Expect = 0.0
Identity = 668/737 (90.64%), Postives = 702/737 (95.25%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           M DTE+ SHPLLR+RRT+LNH FSSSQMEAMAAFCEALIP +PL KETPLDQSL+SFYQ+
Sbjct: 1   MADTEIRSHPLLRKRRTELNHGFSSSQMEAMAAFCEALIPSLPLSKETPLDQSLISFYQA 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SA + PIPDEVA+L+ ERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPF LKFS
Sbjct: 61  SASEPPIPDEVAELMAERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFILKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EIS+EKRE ILKNWSMAH KYTVLLRMTFMIIKIFCCFKFFSRID  TKNVAWK MGY V
Sbjct: 121 EISVEKREEILKNWSMAHQKYTVLLRMTFMIIKIFCCFKFFSRIDGETKNVAWKAMGYHV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRK+LN T+KERPLERGMIETGNEDDST  +SL+ KGLEV EDKKHGNAYKIKCD+VIV
Sbjct: 181 DTRKRLNDTRKERPLERGMIETGNEDDSTFPKSLTLKGLEVTEDKKHGNAYKIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPKS+LDEWSTD+KIPL+ SIDYQLAMDAVCDRIGV E C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLFRSIDYQLAMDAVCDRIGVIEGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            +ESFQNQVLRKGCENLGLKVD VSRNSSKDHYCGSCCYGCRVGDKKGTD+TWLVDAVE 
Sbjct: 361 SLESFQNQVLRKGCENLGLKVDLVSRNSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVES 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGCQADKFILENTNNTS+ KRC+GVIATP SKN+TKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCQADKFILENTNNTSRNKRCLGVIATPLSKNITKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIG+NLHLHPV L WGYFPEELSDF GKIYEGGLITSLHKVVSK S+ H
Sbjct: 481 PLLIASGLRNKNIGSNLHLHPVILAWGYFPEELSDFTGKIYEGGLITSLHKVVSKDSHSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AIIETPA+G ASFAALFPW SGREMKDAMVKY+RTAHLFAL+RDQGAGEIKVKANVKYRL
Sbjct: 541 AIIETPALGSASFAALFPWISGREMKDAMVKYARTAHLFALVRDQGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRI+CQGTNKE+LEEFLDSVEAVGGP
Sbjct: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIKCQGTNKEDLEEFLDSVEAVGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
           LSR+K+WTMYCSAHQMSSC+MGST+EEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI
Sbjct: 661 LSREKHWTMYCSAHQMSSCKMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 737
           TIQSTAYCISKKIAESL
Sbjct: 721 TIQSTAYCISKKIAESL 737

BLAST of Chy4G078160 vs. NCBI nr
Match: XP_022967423.1 (long-chain-alcohol oxidase FAO2-like [Cucurbita maxima] >XP_022967424.1 long-chain-alcohol oxidase FAO2-like [Cucurbita maxima])

HSP 1 Score: 1300 bits (3363), Expect = 0.0
Identity = 637/737 (86.43%), Postives = 689/737 (93.49%), Query Frame = 0

Query: 1   MEDTEVPSHPLLRRRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQS 60
           MED EV SHPLLRRRRT+L+H FSSSQMEAMAAFCEALIPP+PL KE PLDQSLL+FY++
Sbjct: 1   MEDREVRSHPLLRRRRTELSHGFSSSQMEAMAAFCEALIPPLPLVKEDPLDQSLLAFYEA 60

Query: 61  SAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFS 120
           SA QAPIPDEVA LLV+RANPKAVFLVKLVL LLSFRIGTLLLCG +CFDWRWPF LKFS
Sbjct: 61  SASQAPIPDEVAALLVDRANPKAVFLVKLVLMLLSFRIGTLLLCGYLCFDWRWPFVLKFS 120

Query: 121 EISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKV 180
           EIS EKRE+ILK+WS+AHH+YTVLLRM FMIIK+FCCFKFFSRID  +KN+AW+ MGY V
Sbjct: 121 EISPEKREQILKDWSVAHHRYTVLLRMVFMIIKMFCCFKFFSRIDRESKNIAWEAMGYHV 180

Query: 181 DTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIV 240
           DTRK+LN ++KERPLERG+IETG EDDSTLLRSLS KGL V ED+KHG+ Y IKCD+VIV
Sbjct: 181 DTRKRLNDSRKERPLERGLIETGKEDDSTLLRSLSMKGLAVTEDQKHGSTYTIKCDVVIV 240

Query: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVM 300
           GSGCGGGVAAAVLAKSGLKVVVLEKGNYFV EDYSSLEGPSMNELYE+GGLKSTLDGKVM
Sbjct: 241 GSGCGGGVAAAVLAKSGLKVVVLEKGNYFVPEDYSSLEGPSMNELYETGGLKSTLDGKVM 300

Query: 301 LLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEEC 360
           LLAGTTVGGGSAINWSASIRTPK +L+EWST +KIPL+GS DYQ AMDAVCDRIGVTE C
Sbjct: 301 LLAGTTVGGGSAINWSASIRTPKPVLEEWSTIHKIPLFGSFDYQSAMDAVCDRIGVTEGC 360

Query: 361 PVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY 420
            +ESFQNQVLRKGCENLGLKVDSV RNSSKDHYCGSCCYGCRVGDKKGTD+TWLVDAVE 
Sbjct: 361 RLESFQNQVLRKGCENLGLKVDSVPRNSSKDHYCGSCCYGCRVGDKKGTDSTWLVDAVES 420

Query: 421 GAVILTGCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTP 480
           GAVILTGC+ADK +LENTNNTS+ KRC+GVIATP SKNVTKKLHIEAKATISACGSLLTP
Sbjct: 421 GAVILTGCRADKLVLENTNNTSRNKRCLGVIATPLSKNVTKKLHIEAKATISACGSLLTP 480

Query: 481 PLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNCH 540
           PLLIASGLRNKNIG+NLHLHPV L WGYFPE+LSDF GKIYEGGLITSLH VVSK SN H
Sbjct: 481 PLLIASGLRNKNIGSNLHLHPVILAWGYFPEQLSDFTGKIYEGGLITSLHNVVSKDSNSH 540

Query: 541 AIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRL 600
           AII+TPA+GPASFAA+FPWTSGR+MKDA+VKY+RTAHLFAL+RD+GAGEIKVKANVKYRL
Sbjct: 541 AIIQTPALGPASFAAMFPWTSGRDMKDAVVKYARTAHLFALVRDRGAGEIKVKANVKYRL 600

Query: 601 NQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGP 660
           +QTDKENLRIGLRQALRILIAAGATEVGTYRSDGQR +  GTNKE+LEEF DSVEA GGP
Sbjct: 601 DQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRFQFHGTNKEDLEEFFDSVEAAGGP 660

Query: 661 LSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMI 720
            SR+KYW +YCSAHQMSSCRMGSTEEEGAVDENGESWEA+GLFVCDGSVLPTA+GVNPMI
Sbjct: 661 FSREKYWNIYCSAHQMSSCRMGSTEEEGAVDENGESWEAKGLFVCDGSVLPTAVGVNPMI 720

Query: 721 TIQSTAYCISKKIAESL 737
           TIQSTAYCISKK+AESL
Sbjct: 721 TIQSTAYCISKKLAESL 737

BLAST of Chy4G078160 vs. TAIR 10
Match: AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 867.1 bits (2239), Expect = 1.0e-251
Identity = 430/738 (58.27%), Postives = 553/738 (74.93%), Query Frame = 0

Query: 9   HPLLR--RRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFKETPLDQSLLSFYQSSAFQAP 68
           HPLLR  +R    NH FS SQ++++A  C+  +PP    + T   Q++ SF+ +S+ Q P
Sbjct: 11  HPLLRSKKRGEGYNHGFSPSQIQSLAVICQTFLPP----ETTSEQQAVNSFHVASSTQPP 70

Query: 69  IPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFSEISLEK 128
             DEVA+++V+    +AV +++++L +LSFR GTLLLCG++C D  WPF LKFS++ L+K
Sbjct: 71  FTDEVAEMIVKNGRSEAVKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDK 130

Query: 129 RERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKVD----- 188
           RE IL+NWS     + +  R+TF + K +  F FFS+ DE  KN A + +GY +D     
Sbjct: 131 REAILRNWS-RQSGFLLPFRITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTERS 190

Query: 189 TRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIVG 248
           + K+    +K RPLE+G+IET +E D T+ +SL++KG+ V  D    N Y+I+CD V+VG
Sbjct: 191 SNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVARD-DGDNVYRIRCDAVVVG 250

Query: 249 SGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVML 308
           SG GGGVAAA LAK+GLKV+VLEKGNYF A DYS LE PSM ELYE GGL +T+DGK ML
Sbjct: 251 SGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVDGKFML 310

Query: 309 LAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECP 368
           LAG+ VGGG+A+NWSASIRTP  +L EWS   KI  +GS +YQ AMD V  RIGVTE C 
Sbjct: 311 LAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGVTERCV 370

Query: 369 VESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEYG 428
              FQNQVLRKGCE LGL+V+SV RNS +DHYCG C YGCR G K GTD TWLVDAVE G
Sbjct: 371 KHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVDAVENG 430

Query: 429 AVILTGCQADKFIL-ENTNNTSKR-KRCIGVIATPRSKNVTKKLHIEAKATISACGSLLT 488
           AVILTG +A++F+L +NT+++++R KRC+GV A+     + KK  IEA+ T+S+ GSLLT
Sbjct: 431 AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSSAGSLLT 490

Query: 489 PPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVVSKYSNC 548
           PPL+++SGL+N NIG NL LHPV + WGYFPE+ S+F GK+YEGG+ITS+H +    S C
Sbjct: 491 PPLMLSSGLKNPNIGRNLKLHPVLMTWGYFPEKDSEFSGKMYEGGIITSVHHMNDTESGC 550

Query: 549 HAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYR 608
            AI+E P +GPAS+A L PW SG ++K+ M+KY RTAHLFAL+RD G+GE+ ++  V YR
Sbjct: 551 KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDLGSGEVMMENEVTYR 610

Query: 609 LNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGG 668
             + D+ENLR GLRQALR+ +AAGA EVGTYRSDGQ+++C+   KE +EEFLD V+AVGG
Sbjct: 611 TTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKEAMEEFLDEVDAVGG 670

Query: 669 PLSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPM 728
             ++ +YWT Y SAHQM SCRMG T EEGA+DENGESWEAEGLFVCDGS+LP+A+GVNPM
Sbjct: 671 VGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVCDGSILPSAVGVNPM 730

Query: 729 ITIQSTAYCISKKIAESL 738
           ITIQSTAYCIS KI +SL
Sbjct: 731 ITIQSTAYCISSKIVDSL 742

BLAST of Chy4G078160 vs. TAIR 10
Match: AT3G23410.1 (fatty alcohol oxidase 3 )

HSP 1 Score: 814.3 bits (2102), Expect = 8.0e-236
Identity = 404/734 (55.04%), Postives = 541/734 (73.71%), Query Frame = 0

Query: 22  DFSSSQMEAMAAFCEALIPPV-PLFKETPLD-------QSLLSFYQSSAFQAPIPDEVAD 81
           D + ++ME++A+FCEA++P V P  +E   +       ++L SFY +S  + P+  +  +
Sbjct: 14  DITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIE 73

Query: 82  LLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFSEISLEKRERIL-- 141
           L+ +R   +A    +L+L LL+ R+GTLL+CG  C   RWPF  KFSE+SLEKRER+L  
Sbjct: 74  LVTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQK 133

Query: 142 --KNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYKVD-TRKQLNH 201
             KNW +        +R  F+ IK+   F FFSR++   +N AW+ +GY+V+    + + 
Sbjct: 134 QFKNWILTP------IRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSE 193

Query: 202 TQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLVIVGSGCGGGV 261
           T  ERPLE+G++ET  E + TLL SL+ KGLE + D +H +A +IKCD+V+VGSG GGGV
Sbjct: 194 THNERPLEKGIVETMEETEQTLLESLAHKGLEAVLDTEH-DAIRIKCDVVVVGSGSGGGV 253

Query: 262 AAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTVG 321
           AA+VLAKSGLKVVVLEKG+YF   ++   EGP +++LYE+GG+  ++DG  M+LAG TVG
Sbjct: 254 AASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGGILPSVDGSFMVLAGATVG 313

Query: 322 GGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECPVESFQNQ 381
           GGSA+NWSA I+TPKS+L EWS D  IPL+G+ +Y  AM+ V  R+GVTE+C +ESFQNQ
Sbjct: 314 GGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVVWKRMGVTEKCELESFQNQ 373

Query: 382 VLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEYGAVILTGC 441
           +LRKGCENLG  V++V RNSS+ HYCGSC YGCR GDKKG+D TWLVDAV +GAVILTGC
Sbjct: 374 ILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSDRTWLVDAVGHGAVILTGC 433

Query: 442 QADKFILE--NTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTPPLLIAS 501
           +A++FILE   +N   K+ +C+GV+A   + N+ K L IEAK T+SA G+LLTPPL+I+S
Sbjct: 434 KAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAKMLKIEAKVTVSAGGALLTPPLMISS 493

Query: 502 GLRNKNIGTNLHLHPVSLVWGYFPEELS---DFKGKIYEGGLITSLHKVVSKYSNCHAII 561
           GLRN+NIG NLHLHPV + WGYFP++ S    FKG  YEGG+ITS+ KV+S+ S   AII
Sbjct: 494 GLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFKGNSYEGGIITSVSKVLSEDSEVRAII 553

Query: 562 ETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRLNQT 621
           ETP +GP SF+ L PWTSG +MK  M +YSRTA L  ++RD+G+GE+K +  + Y +++T
Sbjct: 554 ETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASLITIVRDRGSGEVKTEGRINYTVDKT 613

Query: 622 DKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGPLSR 681
           D++NL+ GLR++LRILIAAGA EVGT+RSDGQR+ C+G N+  ++EFLDSV    G    
Sbjct: 614 DRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLICKGVNENSIQEFLDSVSTEEGAKGM 673

Query: 682 QKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMITIQ 738
            + W +Y SAHQM SCR+G  E+EGA+D NGESWEAE LFVCD S LP+A+GVNPMIT+ 
Sbjct: 674 TEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWEAEKLFVCDASALPSAVGVNPMITVM 733

BLAST of Chy4G078160 vs. TAIR 10
Match: AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 806.2 bits (2081), Expect = 2.2e-233
Identity = 407/743 (54.78%), Postives = 540/743 (72.68%), Query Frame = 0

Query: 10  PLLR--RRRTDLNHDFSSSQMEAMAAFCEALIPPVPLFK-------ETPLDQSLLSFYQS 69
           PLLR   ++   +H FS S ++A+++ C+A++PPVPL         +   + +LLSF++S
Sbjct: 13  PLLRWSVKQESFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLRNDALLSFFKS 72

Query: 70  SAFQAPI-PDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWR-WPFFLK 129
           S+ ++ + PDEVA+LL  +A P  V +V++VLR+L+FR+GTLLLCG VC D + WPF LK
Sbjct: 73  SSSESHVRPDEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFLLK 132

Query: 130 FSEISLEKRERILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDEGTKNVAWKGMGY 189
           FSE+SLEKRE++L+ W+     Y  L R+ FM+IK    F +F+  +E ++N  W  + Y
Sbjct: 133 FSEMSLEKREKVLQRWNT--QWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAINY 192

Query: 190 KVDTRKQLNHTQKERPLERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGNAYKIKCDLV 249
            V+  +  +  QKERPL+ G+IET  ED+ T+ + +  KGL+V ED++  + YKI+CD V
Sbjct: 193 SVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVTEDRER-DTYKIECDAV 252

Query: 250 IVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGK 309
           +VGSGCGGGVAAA+LAKSGL+VVV+EKGNYF   DYS+LEGPSM EL+ES  L  T DG+
Sbjct: 253 VVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFELFESNSLMMTHDGR 312

Query: 310 VMLLAGTTVGGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTE 369
              +AG+TVGGGS +NW+AS++TP +I++EWS    I +Y S  Y+ AM  VC R+GVTE
Sbjct: 313 FRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYKAAMGIVCKRLGVTE 372

Query: 370 ECPVESFQNQVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAV 429
           +   E FQNQ+LRKGCE LGL V  V RNS++ HYCGSC YGC  G+K+GTD+TWLVDAV
Sbjct: 373 KIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTGEKRGTDSTWLVDAV 432

Query: 430 EYGAVILTGCQADKFILENTN-----NTSKRKRCIGVIATPRSKNVTKKLHIEAKATISA 489
              AVILT C+A+K IL + +      + +RKRC+GV A+  S    KKL I AK TI A
Sbjct: 433 NNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAAS-LSHQTRKKLQINAKVTIVA 492

Query: 490 CGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITSLHKVV 549
           CGSL TP LL +SGL+N NI   LH+HP+ + WGYFPE+ S+ +G  +EG ++TSLH V 
Sbjct: 493 CGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNSELEGAAHEGEIVTSLHYVH 552

Query: 550 SKYSNCHAI-IETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKV 609
              S    I +ETPA+GP +FAAL PW SG +MK+ M KY+RTAH+FA++RD+G GE+K 
Sbjct: 553 PMDSTTPNITLETPAIGPGTFAALTPWVSGSDMKERMAKYARTAHIFAMVRDEGVGEVKG 612

Query: 610 KANVKYRLNQTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLD 669
              VKYRL + D+ENL IGL+QALRIL+AAGA EVGTYRSDGQR++C G  +++LE FLD
Sbjct: 613 DI-VKYRLTKADEENLTIGLKQALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAFLD 672

Query: 670 SVEAVGGPLSRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPT 729
           +V A  G +S  K+WT   +AHQ+  CRMG+TE+EGA+D  GESWEAE L+VCD SVLPT
Sbjct: 673 TVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEKEGAIDGKGESWEAEDLYVCDASVLPT 732

Query: 730 AIGVNPMITIQSTAYCISKKIAE 736
           A+GVNPMIT+QSTAYCIS +IAE
Sbjct: 733 ALGVNPMITVQSTAYCISNRIAE 750

BLAST of Chy4G078160 vs. TAIR 10
Match: AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 576.6 bits (1485), Expect = 2.8e-164
Identity = 319/736 (43.34%), Postives = 452/736 (61.41%), Query Frame = 0

Query: 28  MEAMAAFCEALIPPVPLFKETPLDQSLLSFYQSSAFQAPIPDEVADLLVERANPKAVFLV 87
           ME++ A C+  I  +       +D  +  ++ +SA Q   PD VA L+ ER +    +++
Sbjct: 1   MESLVAICDTFISSIDDSGVGHVDDCVAGYFSASASQTGTPDRVARLMSERLHHPKKWIL 60

Query: 88  KLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFSEISLEKRERILKNWSMAHHKYTVLLRM 147
           +  L LLS  IG+L+LCG   F   +P+F +F  +  ++RE IL NWS +   Y  LLRM
Sbjct: 61  RAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSS---YFSLLRM 120

Query: 148 TFMIIKIFCCFKFFSRIDEGTKNVAWKGMGYK------VDTRKQLNHTQKER-------P 207
            F  IK+     FF+++DE  +N+AWK +GY        D   +LN  +K++       P
Sbjct: 121 LFRTIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPDHSDHEVELNEEKKKKKPEEIFGP 180

Query: 208 LERGMIETGNEDDSTLLRSLSQKGLEVIEDKKHGN-------AYKIKCDLVIVGSGCGGG 267
           L  G+++  +  ++ + + L+ +G  V   K++ N         KI+CD V+VGSG GGG
Sbjct: 181 LYNGIVDLKSPREA-VEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGSGSGGG 240

Query: 268 VAAAVLAKSGLKVVVLEKGNYFVAEDYSSLEGPSMNELYESGGLKSTLDGKVMLLAGTTV 327
           VAA VLAK+G KV+V+E GNY+     S LEG +M+++Y SGGL +T D  V++LAG+TV
Sbjct: 241 VAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVILAGSTV 300

Query: 328 GGGSAINWSASIRTPKSILDEWSTDYKIPLYGSIDYQLAMDAVCDRIGVTEECPVESFQN 387
           GGGS INWSASI+TP+ ++ EW+   K+ ++GS  Y+ AMD VC R+GV      E F N
Sbjct: 301 GGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFVEEGFNN 360

Query: 388 QVLRKGCENLGLKVDSVSRNSSKDHYCGSCCYGCRVGDKKGTDTTWLVDAVEY-GAVILT 447
           +VLRKGCE LGL V ++ RN+  DHYCG CC GC+ G K+GT  TWLVD VE    +IL 
Sbjct: 361 EVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESDNGLILP 420

Query: 448 GCQADKFILENTNNTSKRKRCIGVIATPRSKNVTKKLHIEAKATISACGSLLTPPLLIAS 507
           GCQA + + +      K+K+  GV      +       +E++ TI ACG+L TP LL  S
Sbjct: 421 GCQATEVMYD--CEQGKKKKATGVAFAFGEEIYV----VESRVTIVACGALRTPHLLKRS 480

Query: 508 GLRNKNIGTNLHLHPVSLVWGYFPEE--LSDFKGKIYEGGLITSLHKVVSKYSNC---HA 567
           GL+N NIG NL LHPV + WG+FPEE    + K K YEGG++T++  VV + ++      
Sbjct: 481 GLKNSNIGRNLCLHPVVMAWGWFPEEDKWPEKKKKSYEGGIMTAMSSVVIEETHSSYGEM 540

Query: 568 IIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALIRDQGAGEIKVKANVKYRLN 627
           +I+TPA+ P  F+ + PWTS ++ K  M+K+SRTAH+FAL+RD+G G I  K  + Y LN
Sbjct: 541 VIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSKTYIDYNLN 600

Query: 628 QTDKENLRIGLRQALRILIAAGATEVGTYRSDGQRIRCQGTNKEELEEFLDSVEAVGGPL 687
             D+E+L+ GL + L+IL AAGA E+GT+ S+G+ +  +  +  E+E F+   E    PL
Sbjct: 601 DEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERFVR--EESSKPL 660

Query: 688 SRQKYWTMYCSAHQMSSCRMGSTEEEGAVDENGESWEAEGLFVCDGSVLPTAIGVNPMIT 738
             +      CSAHQM SCRMG   EE AV   GE+WE E LFV D SV PTA+GVNPM+T
Sbjct: 661 --KDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTALGVNPMVT 720

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B5WWZ92.7e-27363.84Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1[more]
Q94BP31.5e-25058.27Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 S... [more]
B5WWZ87.0e-24556.06Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1[more]
Q9LW561.1e-23455.04Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=... [more]
Q9ZWB93.1e-23254.78Long-chain-alcohol oxidase FAO1 OS=Arabidopsis thaliana OX=3702 GN=FAO1 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LBV70.0e+0098.78Long-chain-alcohol oxidase OS=Cucumis sativus OX=3659 GN=Csa_3G575330 PE=3 SV=1[more]
A0A5A7T3R30.0e+0095.52Long-chain-alcohol oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3BHB10.0e+0095.39Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103489850 PE=3 SV=1[more]
A0A6J1HWN70.0e+0086.43Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111466966 PE=3 SV=1[more]
A0A6J1HG830.0e+0085.62Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111464000 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_004152057.10.098.78long-chain-alcohol oxidase FAO2 [Cucumis sativus] >KGN58167.1 hypothetical prote... [more]
KAA0037980.10.095.52long-chain-alcohol oxidase FAO2-like [Cucumis melo var. makuwa][more]
XP_008447391.20.095.39PREDICTED: LOW QUALITY PROTEIN: long-chain-alcohol oxidase FAO2-like [Cucumis me... [more]
XP_038888257.10.090.64long-chain-alcohol oxidase FAO2-like [Benincasa hispida][more]
XP_022967423.10.086.43long-chain-alcohol oxidase FAO2-like [Cucurbita maxima] >XP_022967424.1 long-cha... [more]
Match NameE-valueIdentityDescription
AT4G28570.11.0e-25158.27Long-chain fatty alcohol dehydrogenase family protein [more]
AT3G23410.18.0e-23655.04fatty alcohol oxidase 3 [more]
AT1G03990.12.2e-23354.78Long-chain fatty alcohol dehydrogenase family protein [more]
AT4G19380.12.8e-16443.34Long-chain fatty alcohol dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 220..530
e-value: 3.6E-28
score: 100.5
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 574..736
e-value: 2.8E-15
score: 58.4
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 233..735
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 593..728
e-value: 5.7E-23
score: 82.0
IPR012400Long-chain-alcohol oxidasePIRSFPIRSF028937Lg_Ch_AOcoord: 12..737
e-value: 1.6E-303
score: 1006.5
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 237..502
e-value: 9.0E-66
score: 222.2
NoneNo IPR availablePIRSRPIRSR028937-2PIRSR028937-2coord: 8..737
e-value: 0.0
score: 1183.8
NoneNo IPR availablePIRSRPIRSR028937-1PIRSR028937-1coord: 8..737
e-value: 0.0
score: 1183.8
NoneNo IPR availablePANTHERPTHR46056:SF12LONG-CHAIN-ALCOHOL OXIDASE FAO4Bcoord: 7..737
NoneNo IPR availablePANTHERPTHR46056LONG-CHAIN-ALCOHOL OXIDASEcoord: 7..737

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G078160.1Chy4G078160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046577 long-chain-alcohol oxidase activity
molecular_function GO:0046593 mandelonitrile lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors