Chy4G077320 (gene) Cucumber (hystrix) v1

Overview
NameChy4G077320
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAutophagy-related protein 18f
LocationchrH04: 14952725 .. 14956875 (+)
RNA-Seq ExpressionChy4G077320
SyntenyChy4G077320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTAAAGTGGATGTTGGAGAATTGGTGATTCTTGTATTTTGGGTATTGGCTATTTTTCTTACTTTGATTCTGGGGATGAGGAATGATGGAACTCTGAAACAACAGGGAACTGTAGCCCGCTCTGGCCGGGCTCATGAGTTCTTATCAACTTCCTTTCGTGCTTTTTCTAGTTACATGAAGATTGTTTCTGCTGGTGCATCCACTGTTGCTCGGTCGGCTGCTTCAGTGGCATCTTCTTTGGTGGATAAGGATGATGAAGCAAATGCATTCCAGGTATGTGCCGAACTTTAATGAAACTTCTATCTAATACTTGGAATTGTGACGAGTTCTTGTGTTTTATTTATCTAGTTTGTTGGAAATTTTTTATTTTTGTGGTTTATTTTGTTTCTCTGGTTGAGAGAACGTTATTTGGTACAAGTTTATAATAGGCTGACGCTTGTATAGAGGGGCACAAGGTTTTGATTGCTTTTTATTTGATCGAAAATTGTTTATCTTAATACGGCATTTGAAATTGAAACGTGCACGATCATCACACATGGGTTGCTGGAACTTTTTCTTCGTTTGCAGCTCCCATCTACAATGGCTTTGTCTTTTGGAATTAATATTGTTACTTTATTGTCAACGTTGTAGCTCATCCATGTATTGACCTTGATTTTCACAATCTATCGCAATACTACTCCATGTTCTTGATAGCTCAAAATTGAGAAATCCTGCCATTTCTCTATTATATGATTGTTGAACAAAAAAGAAGCTGTTAGTTTAAAAGCTTTATTGATTAAGCAATGGAAGCTTTGTGAAATTTTCTTGGAATTAGAAATAAGTTGTGCTTCAGTGGTCCAATTATATTTTCATCCTTGTATGATACATGATCATTGGTCTTTTGTGTCTGTATCGGTCTAAAATGGAACTGTAAATTTATAACTCTTTAGTTTTAGTTGTAGTATAAATTTCCAAAATCAGCAACCTTATATTCCACTTTGTTTATAAATCTAGTTATCTTTATCTGCATAATGTTAACATTGTTTCTAGTGTTCGAATTTTAATAAATGGGTGATTATTGATTCCTAATTCCATTGCTAAATGATAAAGGTGAATTGGGCTGGCTTTGACAAGTTAGAGTGGGGCGACAATGTCATTCGACAGGTTCTTCTGTTGGGTATTCGTTCTGGTTTCCAGGTTTGGGATGTTGAGGAAGCAAATAATGTCCAAGAACTCGTTTGTCGGTATGATGGTTCTGTTTCATACATGCAAGTTTTACCCAGATTAATACTATCAATGAGATCAGGAGATAAGTTTGCAGAAAGTAGGCCATTGGTTGTACTTTCTGCATATGGTTCCATTGCTGCTAATTTTAACATTCAGGATCAATTAGCCTCTTCTGGCAATGCCGCCATCCCAAAGAGCCAGGAACTAGTAGATGGGAATTTTATGCCTACATTTGTTAGGTTTTATTCCTTGAAATCTCAGACTTACGTGCACGAACTTAAATTTAGATCAGCTGTTTATTCAGTGAAATGCAGCCCCCTGGTTGTTGCCATCTCTCTGGCAACTCAGGTATGTTTTCTGCACAAAAGCAGTTGCAGTCTTATTATTTGTTTCTAATCTAATACATTAAAGAACTGTAAACCGTATAGTTGGTAAGAATTTGAGTCCTTTGTATAACAGATACACTGCATCAGTGCTACAACTTTGGAGAAGGAACATATCATTCTTACAAATCCTGTAGTTTCTGGTTTTCCTGGATCTGGAGGAGGTATGGGTTATGGACCCCTCGCATTGGGACCCAGATGGCTGGCTTATAGTGGAAGTCCGATCTTGTTGTCAAATACAGGACGAGTGGTTCCGCAGCATTTAAAACCTTCTGCAAGTTTTTCTCATTCTTCTTCAAATGGAAGTTTAGTTGCTCACTATGCAAAGGAGTCGAGTAAGCATCTTGCAGCAGGCATTGTTACCCTTGGCGACAAGGGTATTAAGAAGCTATCAAGATATTACTCCGAGCTTTTACCTGAAAGCAATAATTCTCTTCAATCAGGTGCTCAGGGTTTGAAGGGCATCGGAACTCTTAATGGCCATATGGCAGATGCAGATAGCATTGGAATGGTCTGTATTGATCTTTAGTTTGTTTCTTACTTTCTTTACCTAATGCGTTGTTTTCTTTTCTTCTTTTTAATGATTTATATTTTGACTATGTTTCAGTTATGCAGCTGAACTGATTCAGTTATGATAGTTAATAAGATCTAATTTTGTTTCTTGCCAATGACCTTGTTATTCCTTTCGACTTTTGGTTTTCAGGCTATTGTGAAAGATATCATCAGCAAAGCGGTCATCACCCAGTTTAAGGCTCATAAGAGCCCAATTTCTGCTTTATGCTTTGATCCAAGTGGCACTATCTTGGTGACTGCTTCTGTTCAGGGTCATAGCATAAATGTTTTCAAAATTATGCCAAGTTCCTGTTCGAAGTCATCTATATCTGGTACTGCTGCTTCTTATAGCCATTTGTACAGACTCCAAAGAGGCTTCACTAATGCAGTAAGAGTGGCAAATTTATTCCATTGTTGTACTAAGTCATCACTTTTTGTCATAATCATATTTTTGGATCTATTGTCAGGTCATACAAGACATCAGTTTCAGTTACGATAGCAACTGGATCATGATAAGTTCTTCTAGAGGGACAAGCCATCTTTTTGCTATAAACCCTGCTGGGGGACAGGTAAATTTCCCATCTGCTGTCATTATTGCTAGAAATGGAGAGCCAGTGGTTCCTGCACGACAAACTGTTCGACGAGTTGATTCTGGTTTGCACATGCCTAAACAGAACCAATGCACTACTGGTAGTCCGCTTACACTTTCTGCTGTTACTAGAATACATCATGGAAGTAATGGCTGGCGAGGCACTGTTAGTAGTGCTGCAGCTGCTGCAACTGGTAAAATGGGTATTGTTTCGGGAGCTATTGCTTCAGCTTTCCATGAGTGCAAGGGAAACACTGTGCAAGTGGACAATGGATCTTCTGAGGTTAGGTACCACATATTAGTTTTTTCTCCATCTGGCAGTATGATACAGTATGCATTGCGAGTGGGTTTAGATTCAACCATAGTTCTGCCGAGATCTAGCACAGCTCTTGAATTAGTCTCTGAGTTGGATGCAAGACTAGTAGTTGAAGCTATCCAGAAATGGAATATATCTCAGAAACAAAACCGTAGATCTCAAGATAACAGCATTGACATATACGGTGACGATGGAGGTTTCAATTGCAATAAAAACTATTGTGAGGAAATGAATGGGAACCCTGTCCTTGAAGCTGGTGGCAATATCTTCAAGTCAAAGGCTTGCAGGGAGGAACAATACCACCTGTATATTTCAGAAGCAGAACTGCAGATGCATGCTGCTCGAACTCCGCTGTGGACAAGACCTGAGGTATCTACTGTTCGACTCATGTTTTTAAGTAGGCCTCTCTATGTTTGTTTGAGTTTTACATGCCTGCATTTTTTGTAACTGATGATCAGATATATTTTCAAGTAATGGCAAGAGATGGAGTCAAAATTGATGAAATTGACCATCCAGGTGAACTCGATATTGAAAGGATACCCACTCGTATGATTGAAGCTAGGTCAAAAGACCTAGTTCCAGTTTTTGACTATCTTCAGTCATCGAAAATTTCACAGCCAAGGTGAGCTAAGTTATAGAATTTCTCAAGCTTCCTATTTTTAATTGACTTTGGGGAACCCGAAGATCCTGCCAATAATGCTTACTAATACACAATGCTCGATGTAAGAATTTTTGGTTCTCATCAACATTTTCCCTGAAATCTAGGTATTTAAATAGCAACAGCGACCAGCTGTTGCTGCAACAGAAATCTGGCCAATTTGAAAATGGAAGGCAATCGTGGAGGAGTGCTGCAAATCCCCACGATTCTGTTATTGGGAATGGACGTGAAGTAACCGGTAGGCATGGTTACAAGCGGGTGACAGAAACCAAGGGCATTGTAAATGAGCCTGAATGCCCAAGGTCACAGACCCAGTTCAGTAATGTAAATAGTTGTACAGAGAGCATAAGCATGGAGTCTCAACCCAAATCTGTAAATAATCATCATAAAAGTGGCCTCAGGGTGGAGAATCATTTTGAAGACCTAGGTGATGAGTTTGAGTAG

mRNA sequence

ATGATTAAAGTGGATGTTGGAGAATTGGTGATTCTTGTATTTTGGGTATTGGCTATTTTTCTTACTTTGATTCTGGGGATGAGGAATGATGGAACTCTGAAACAACAGGGAACTGTAGCCCGCTCTGGCCGGGCTCATGAGTTCTTATCAACTTCCTTTCGTGCTTTTTCTAGTTACATGAAGATTGTTTCTGCTGGTGCATCCACTGTTGCTCGGTCGGCTGCTTCAGTGGCATCTTCTTTGGTGGATAAGGATGATGAAGCAAATGCATTCCAGGTGAATTGGGCTGGCTTTGACAAGTTAGAGTGGGGCGACAATGTCATTCGACAGGTTCTTCTGTTGGGTATTCGTTCTGGTTTCCAGGTTTGGGATGTTGAGGAAGCAAATAATGTCCAAGAACTCGTTTGTCGGTATGATGGTTCTGTTTCATACATGCAAGTTTTACCCAGATTAATACTATCAATGAGATCAGGAGATAAGTTTGCAGAAAGTAGGCCATTGGTTGTACTTTCTGCATATGGTTCCATTGCTGCTAATTTTAACATTCAGGATCAATTAGCCTCTTCTGGCAATGCCGCCATCCCAAAGAGCCAGGAACTAGTAGATGGGAATTTTATGCCTACATTTGTTAGGTTTTATTCCTTGAAATCTCAGACTTACGTGCACGAACTTAAATTTAGATCAGCTGTTTATTCAGTGAAATGCAGCCCCCTGGTTGTTGCCATCTCTCTGGCAACTCAGATACACTGCATCAGTGCTACAACTTTGGAGAAGGAACATATCATTCTTACAAATCCTGTAGTTTCTGGTTTTCCTGGATCTGGAGGAGGTATGGGTTATGGACCCCTCGCATTGGGACCCAGATGGCTGGCTTATAGTGGAAGTCCGATCTTGTTGTCAAATACAGGACGAGTGGTTCCGCAGCATTTAAAACCTTCTGCAAGTTTTTCTCATTCTTCTTCAAATGGAAGTTTAGTTGCTCACTATGCAAAGGAGTCGAGTAAGCATCTTGCAGCAGGCATTGTTACCCTTGGCGACAAGGGTATTAAGAAGCTATCAAGATATTACTCCGAGCTTTTACCTGAAAGCAATAATTCTCTTCAATCAGGTGCTCAGGGTTTGAAGGGCATCGGAACTCTTAATGGCCATATGGCAGATGCAGATAGCATTGGAATGGCTATTGTGAAAGATATCATCAGCAAAGCGGTCATCACCCAGTTTAAGGCTCATAAGAGCCCAATTTCTGCTTTATGCTTTGATCCAAGTGGCACTATCTTGGTGACTGCTTCTGTTCAGGGTCATAGCATAAATGTTTTCAAAATTATGCCAAGTTCCTGTTCGAAGTCATCTATATCTGGTACTGCTGCTTCTTATAGCCATTTGTACAGACTCCAAAGAGGCTTCACTAATGCAGTCATACAAGACATCAGTTTCAGTTACGATAGCAACTGGATCATGATAAGTTCTTCTAGAGGGACAAGCCATCTTTTTGCTATAAACCCTGCTGGGGGACAGGTAAATTTCCCATCTGCTGTCATTATTGCTAGAAATGGAGAGCCAGTGGTTCCTGCACGACAAACTGTTCGACGAGTTGATTCTGGTTTGCACATGCCTAAACAGAACCAATGCACTACTGGTAGTCCGCTTACACTTTCTGCTGTTACTAGAATACATCATGGAAGTAATGGCTGGCGAGGCACTGTTAGTAGTGCTGCAGCTGCTGCAACTGGTAAAATGGGTATTGTTTCGGGAGCTATTGCTTCAGCTTTCCATGAGTGCAAGGGAAACACTGTGCAAGTGGACAATGGATCTTCTGAGGTTAGGTACCACATATTAGTTTTTTCTCCATCTGGCAGTATGATACAGTATGCATTGCGAGTGGGTTTAGATTCAACCATAGTTCTGCCGAGATCTAGCACAGCTCTTGAATTAGTCTCTGAGTTGGATGCAAGACTAGTAGTTGAAGCTATCCAGAAATGGAATATATCTCAGAAACAAAACCGTAGATCTCAAGATAACAGCATTGACATATACGGTGACGATGGAGGTTTCAATTGCAATAAAAACTATTGTGAGGAAATGAATGGGAACCCTGTCCTTGAAGCTGGTGGCAATATCTTCAAGTCAAAGGCTTGCAGGGAGGAACAATACCACCTGTATATTTCAGAAGCAGAACTGCAGATGCATGCTGCTCGAACTCCGCTGTGGACAAGACCTGAGATATATTTTCAAGTAATGGCAAGAGATGGAGTCAAAATTGATGAAATTGACCATCCAGGTGAACTCGATATTGAAAGGATACCCACTCGTATGATTGAAGCTAGGTCAAAAGACCTAGTTCCAGTTTTTGACTATCTTCAGTCATCGAAAATTTCACAGCCAAGGTATTTAAATAGCAACAGCGACCAGCTGTTGCTGCAACAGAAATCTGGCCAATTTGAAAATGGAAGGCAATCGTGGAGGAGTGCTGCAAATCCCCACGATTCTGTTATTGGGAATGGACGTGAAGTAACCGGTAGGCATGGTTACAAGCGGGTGACAGAAACCAAGGGCATTGTAAATGAGCCTGAATGCCCAAGGTCACAGACCCAGTTCAGTAATGTAAATAGTTGTACAGAGAGCATAAGCATGGAGTCTCAACCCAAATCTGTAAATAATCATCATAAAAGTGGCCTCAGGGTGGAGAATCATTTTGAAGACCTAGGTGATGAGTTTGAGTAG

Coding sequence (CDS)

ATGATTAAAGTGGATGTTGGAGAATTGGTGATTCTTGTATTTTGGGTATTGGCTATTTTTCTTACTTTGATTCTGGGGATGAGGAATGATGGAACTCTGAAACAACAGGGAACTGTAGCCCGCTCTGGCCGGGCTCATGAGTTCTTATCAACTTCCTTTCGTGCTTTTTCTAGTTACATGAAGATTGTTTCTGCTGGTGCATCCACTGTTGCTCGGTCGGCTGCTTCAGTGGCATCTTCTTTGGTGGATAAGGATGATGAAGCAAATGCATTCCAGGTGAATTGGGCTGGCTTTGACAAGTTAGAGTGGGGCGACAATGTCATTCGACAGGTTCTTCTGTTGGGTATTCGTTCTGGTTTCCAGGTTTGGGATGTTGAGGAAGCAAATAATGTCCAAGAACTCGTTTGTCGGTATGATGGTTCTGTTTCATACATGCAAGTTTTACCCAGATTAATACTATCAATGAGATCAGGAGATAAGTTTGCAGAAAGTAGGCCATTGGTTGTACTTTCTGCATATGGTTCCATTGCTGCTAATTTTAACATTCAGGATCAATTAGCCTCTTCTGGCAATGCCGCCATCCCAAAGAGCCAGGAACTAGTAGATGGGAATTTTATGCCTACATTTGTTAGGTTTTATTCCTTGAAATCTCAGACTTACGTGCACGAACTTAAATTTAGATCAGCTGTTTATTCAGTGAAATGCAGCCCCCTGGTTGTTGCCATCTCTCTGGCAACTCAGATACACTGCATCAGTGCTACAACTTTGGAGAAGGAACATATCATTCTTACAAATCCTGTAGTTTCTGGTTTTCCTGGATCTGGAGGAGGTATGGGTTATGGACCCCTCGCATTGGGACCCAGATGGCTGGCTTATAGTGGAAGTCCGATCTTGTTGTCAAATACAGGACGAGTGGTTCCGCAGCATTTAAAACCTTCTGCAAGTTTTTCTCATTCTTCTTCAAATGGAAGTTTAGTTGCTCACTATGCAAAGGAGTCGAGTAAGCATCTTGCAGCAGGCATTGTTACCCTTGGCGACAAGGGTATTAAGAAGCTATCAAGATATTACTCCGAGCTTTTACCTGAAAGCAATAATTCTCTTCAATCAGGTGCTCAGGGTTTGAAGGGCATCGGAACTCTTAATGGCCATATGGCAGATGCAGATAGCATTGGAATGGCTATTGTGAAAGATATCATCAGCAAAGCGGTCATCACCCAGTTTAAGGCTCATAAGAGCCCAATTTCTGCTTTATGCTTTGATCCAAGTGGCACTATCTTGGTGACTGCTTCTGTTCAGGGTCATAGCATAAATGTTTTCAAAATTATGCCAAGTTCCTGTTCGAAGTCATCTATATCTGGTACTGCTGCTTCTTATAGCCATTTGTACAGACTCCAAAGAGGCTTCACTAATGCAGTCATACAAGACATCAGTTTCAGTTACGATAGCAACTGGATCATGATAAGTTCTTCTAGAGGGACAAGCCATCTTTTTGCTATAAACCCTGCTGGGGGACAGGTAAATTTCCCATCTGCTGTCATTATTGCTAGAAATGGAGAGCCAGTGGTTCCTGCACGACAAACTGTTCGACGAGTTGATTCTGGTTTGCACATGCCTAAACAGAACCAATGCACTACTGGTAGTCCGCTTACACTTTCTGCTGTTACTAGAATACATCATGGAAGTAATGGCTGGCGAGGCACTGTTAGTAGTGCTGCAGCTGCTGCAACTGGTAAAATGGGTATTGTTTCGGGAGCTATTGCTTCAGCTTTCCATGAGTGCAAGGGAAACACTGTGCAAGTGGACAATGGATCTTCTGAGGTTAGGTACCACATATTAGTTTTTTCTCCATCTGGCAGTATGATACAGTATGCATTGCGAGTGGGTTTAGATTCAACCATAGTTCTGCCGAGATCTAGCACAGCTCTTGAATTAGTCTCTGAGTTGGATGCAAGACTAGTAGTTGAAGCTATCCAGAAATGGAATATATCTCAGAAACAAAACCGTAGATCTCAAGATAACAGCATTGACATATACGGTGACGATGGAGGTTTCAATTGCAATAAAAACTATTGTGAGGAAATGAATGGGAACCCTGTCCTTGAAGCTGGTGGCAATATCTTCAAGTCAAAGGCTTGCAGGGAGGAACAATACCACCTGTATATTTCAGAAGCAGAACTGCAGATGCATGCTGCTCGAACTCCGCTGTGGACAAGACCTGAGATATATTTTCAAGTAATGGCAAGAGATGGAGTCAAAATTGATGAAATTGACCATCCAGGTGAACTCGATATTGAAAGGATACCCACTCGTATGATTGAAGCTAGGTCAAAAGACCTAGTTCCAGTTTTTGACTATCTTCAGTCATCGAAAATTTCACAGCCAAGGTATTTAAATAGCAACAGCGACCAGCTGTTGCTGCAACAGAAATCTGGCCAATTTGAAAATGGAAGGCAATCGTGGAGGAGTGCTGCAAATCCCCACGATTCTGTTATTGGGAATGGACGTGAAGTAACCGGTAGGCATGGTTACAAGCGGGTGACAGAAACCAAGGGCATTGTAAATGAGCCTGAATGCCCAAGGTCACAGACCCAGTTCAGTAATGTAAATAGTTGTACAGAGAGCATAAGCATGGAGTCTCAACCCAAATCTGTAAATAATCATCATAAAAGTGGCCTCAGGGTGGAGAATCATTTTGAAGACCTAGGTGATGAGTTTGAGTAG

Protein sequence

MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIARNGEPVVPARQTVRRVDSGLHMPKQNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQVDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHLYISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGREVTGRHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFEDLGDEFE*
Homology
BLAST of Chy4G077320 vs. ExPASy Swiss-Prot
Match: Q9FH32 (Autophagy-related protein 18f OS=Arabidopsis thaliana OX=3702 GN=ATG18F PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 4.6e-137
Identity = 339/773 (43.86%), Postives = 453/773 (58.60%), Query Frame = 0

Query: 37  GTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDDEANAFQVNWA 96
           G V+RS R      +SFRA S+ +K++S+GASTVARSA S ASS V    E++  QV WA
Sbjct: 15  GVVSRSAR------SSFRALSNCLKVISSGASTVARSAVSAASSAV----ESHHDQVLWA 74

Query: 97  GFDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMR 156
           GFD L+  D   R+VLLL  +SGFQVWDVE+  NV  +V  +DG   +MQ+L   I S  
Sbjct: 75  GFDNLQKEDGDTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV 134

Query: 157 SGDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLK 216
             D+F +SRPL+ +          +   +  SS N   P S+ +      PT V  YSLK
Sbjct: 135 LDDRFYKSRPLLAVCG--------DYSSKKISSDN---PGSETVA----TPTNVYVYSLK 194

Query: 217 SQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNPVVSGFPGSGG 276
           SQ+YVH LKFR+ +YSV+C   +VA+  A QI C  A TLE ++ I+TN +V G      
Sbjct: 195 SQSYVHTLKFRATIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIVCG----SL 254

Query: 277 GMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKH 336
           G+GYGPLA+GPRW+AYSGS I  S++       +  S   S S+S+ S VA +A++SSK 
Sbjct: 255 GVGYGPLAVGPRWIAYSGSRIATSSSA------IFTSEIVSLSTSSPS-VAQFARDSSKQ 314

Query: 337 LAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVK 396
           LA+GI  LGDKG + L++Y SE+LP           GLKGIG  N  +ADA+SIGM IVK
Sbjct: 315 LASGIANLGDKGYRSLTKYCSEVLPNPY------IPGLKGIGVGNEKVADAESIGMVIVK 374

Query: 397 DIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAA 456
           DI +K+VITQFKAHKSPISALCFD SG +LVTAS+QGH+INVF+IMP+  +  ++  T  
Sbjct: 375 DITNKSVITQFKAHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTSRAVKTT-- 434

Query: 457 SYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIAR 516
           +++HL+RLQRGFTNAVIQDI FS DSN I++ SSRGTSHLF INP              +
Sbjct: 435 TFAHLFRLQRGFTNAVIQDICFSKDSNLIVVGSSRGTSHLFEINP-------------EK 494

Query: 517 NGEPVVPARQTVRRVDSGLHMPKQNQCTTGSPLTLSAVTRIHHG-SNGWRGTVSSAAAAA 576
            G+                           +P+ +SA++RI  G S+GW GTVS AA+AA
Sbjct: 495 EGD---------------------------APVPMSAISRIRSGNSSGWIGTVSGAASAA 554

Query: 577 TGKM-GIVSGAIASAFHECKGNTVQVDNGSSE---VRYHILVFSPSGSMIQYALR----- 636
            G + G V G + S F  C   +     GS      + ++LVF+PSG M QYALR     
Sbjct: 555 AGMVAGSVPGTVTSTFCYCDEKSNNNYYGSVADMCSKTNLLVFAPSGCMTQYALREHQVG 614

Query: 637 VGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRR-SQDNSIDIYGDDGGFN 696
           VG ++  +    S   E   E + +L V+ I++W++ Q Q+RR + D   DIYG     +
Sbjct: 615 VGHETAAMTGFDS---ESGLETEGKLAVDPIRRWSMIQNQSRRETHDPHSDIYGGGTSVD 674

Query: 697 CNKNYCEEMNGNPVLEAGGNIFKSKACR-EEQYHLYISEAELQMHAART--PLWTRPEIY 756
                  E+     +E    + K    R  ++ HLYI EAE Q     T  PLW R +  
Sbjct: 675 SKSKVFPEVVRKQSVEEAWKVSKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWARRKFR 698

Query: 757 FQVMARDGVKIDEIDHPG--ELDIERIPTRMIEARSKDLVPVFDYLQSSKISQ 794
           FQ +  +  + +EI   G  E++IE I TR IEAR++DLVPV+ YLQS +  Q
Sbjct: 735 FQELVLN--RGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRSQQ 698

BLAST of Chy4G077320 vs. ExPASy Swiss-Prot
Match: Q8GUL1 (Autophagy-related protein 18g OS=Arabidopsis thaliana OX=3702 GN=ATG18G PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 1.5e-124
Identity = 303/781 (38.80%), Postives = 447/781 (57.23%), Query Frame = 0

Query: 43  GRAHEFLSTSFRAFSSYMKIVSAGASTVARS----AASVASSLVDKDDEANAFQVNWAGF 102
           G+    L  SF+  SS +K VSA A+ VA S     ASVA+S+   +D+ +  QV WAGF
Sbjct: 7   GKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAAEDDKD--QVTWAGF 66

Query: 103 DKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSG 162
             LE G +V R VLLLG ++GFQV+DVE+A+N  ELV +  G VS++Q+ P   L  RSG
Sbjct: 67  GILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQP---LPARSG 126

Query: 163 DK--FAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFM--PTFVRFYS 222
           D   F  S PL+++ A G       +    + +G+ A   S +   G+ +  PT VRFYS
Sbjct: 127 DHEGFWNSHPLLLVVA-GDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINYPTTVRFYS 186

Query: 223 LKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNPVVSGFPGS 282
           L+S +YV+ L+FRS+V  ++CS  VVA+ LA QI+C+ A TLE +  +LT PV    P  
Sbjct: 187 LRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYPVPQ--PVR 246

Query: 283 GG----GMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNG--SLVAH 342
            G     +GYGP+A+GPRWLAY+    +   TGR+ PQ    S S S SSS+G  S +A 
Sbjct: 247 QGTTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGGSSFMAR 306

Query: 343 YAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMADAD 402
           YA ESSK LA G++ LGD G K LS+Y  ++LP+ + S  +    +  +G ++G  +DA+
Sbjct: 307 YAMESSKQLANGLINLGDMGYKTLSKYCQDMLPDGSTS-PASPNAIWKVGGVSG--SDAE 366

Query: 403 SIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSK 462
           + GM  VKD++S A+++QFKAH SPISALCFDPSGT+LVTASV G++INVF+IMPS    
Sbjct: 367 NAGMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNINVFQIMPSRSHN 426

Query: 463 SSISGT---AASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQ 522
           +    +    +S+ HL++L RG T+A++QDI FS  S W+ I SS+GT H+F +N +G  
Sbjct: 427 APGDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGTCHIFVLNSSGSD 486

Query: 523 VNFPSAVIIARNGEPV-VPARQTVRRVDSGLHMPKQNQCTTGSPLTLSAVTRIHHGSNGW 582
             F          EP  +PA          L    Q   +  + + LS V+RI + S GW
Sbjct: 487 AAFQP----CEGEEPTRLPASSLPWWFTQSL-SSNQQSLSPPTAVALSVVSRIKYSSFGW 546

Query: 583 RGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQVDNGSSEVRYHILVFSPSGSMIQYA 642
             TVS+A  AATGK+ + SGA+A+ FH+   + +Q+ N  +    HILV++PSG ++Q+ 
Sbjct: 547 LNTVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQL-NSRTNALEHILVYTPSGHVVQHE 606

Query: 643 LRVGLDSTIV---LPRSSTALELVSELDARLVVEAIQKWNISQKQN---------RRSQD 702
           L   + +      L    T+   V E D R+ VE IQ W++ ++ +         +   +
Sbjct: 607 LLPSVCTESPENGLRVQKTSHVQVQEDDLRVKVEPIQWWDVCRRSDWLETEERLPKSITE 666

Query: 703 NSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACRE---EQYHLYISEAELQMH 762
              D+         +++ C  ++ N        +   K+C E   E+ H Y+S  E+++ 
Sbjct: 667 KQYDLETVSNHLTSHEDACLSLDMNSHFSEDKYL---KSCSEKPPERSHCYLSNFEVKVT 726

Query: 763 AARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDYLQSS 791
           +   P+W   +I F VM  D  + D     GE +IE++P   +E + K L+PVFD+  S+
Sbjct: 727 SGMLPVWQNSKISFHVM--DSPR-DSSSTGGEFEIEKVPAHELEIKQKKLLPVFDHFHST 764

BLAST of Chy4G077320 vs. ExPASy Swiss-Prot
Match: Q8H1Q5 (Autophagy-related protein 18h OS=Arabidopsis thaliana OX=3702 GN=ATG18H PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 8.4e-115
Identity = 279/783 (35.63%), Postives = 430/783 (54.92%), Query Frame = 0

Query: 25  LGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDK 84
           + + N+G    Q    ++   + FL  S +  S+ ++  S+G   V  ++ASVA+SL   
Sbjct: 7   VNINNNGDNHNQ---TKNNGTNGFLPNSLKFISTCIRTASSG---VRSASASVAASL-SS 66

Query: 85  DDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSY 144
           D      QV W+ FD+L   ++  + VLLLG  +GFQV D++++N+V E V R D  V++
Sbjct: 67  DSHELKDQVLWSSFDRLHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTF 126

Query: 145 MQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDG- 204
           +Q+ P L       + F  S P+++            + D+   SG     +   + +G 
Sbjct: 127 LQMQP-LPAKCDGVEGFRSSHPILLA-----------VADEAKGSGPIVTSRDGSVRNGY 186

Query: 205 ----NFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKE 264
                  PT VRFYSL+S  YVH L+FRS VY V+CSP +VA+ L +QI+C  A TLE +
Sbjct: 187 EDPLALSPTVVRFYSLRSHNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENK 246

Query: 265 HIILTNPVVS-GFPG-SGGGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHL-KPSASF 324
             +L+ PV   G  G SG  +GYGP+A+G RWLAY+ +  L S+ GR+ PQ++  P  S 
Sbjct: 247 FSVLSYPVPQLGNQGISGVNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTPPGVSP 306

Query: 325 SHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKG 384
           S S +NG+LVA YA ESSKHLAAG++ LGDKG K +S+Y  +L  +      S + G K 
Sbjct: 307 STSPNNGNLVARYAMESSKHLAAGLLNLGDKGYKTISKYCQDLKHDGPGPSLSSSPGRK- 366

Query: 385 IGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSI 444
           +G +  H A++D +G  IVKD  S+A+I QF+AH SPISALCFDPSGT+LVTAS+ G++I
Sbjct: 367 VGRVGSHSAESDVVGTVIVKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHGNNI 426

Query: 445 NVFKIMPSSCSKSSISGT---AASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGT 504
           NVF+IMP+       + +   ++S+  LY+L RG T+AVIQDI FS  S WI I SS+ T
Sbjct: 427 NVFRIMPTPTKNGPGAQSYDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSSKST 486

Query: 505 SHLFAINPAGGQVNFPSAVIIARNGE---PVVPARQTVRRVDSGLHMPKQNQCTTGSPLT 564
            H++ ++P GG+      V+  RN +   P +    ++    S   M         + +T
Sbjct: 487 CHIYVLSPFGGE-----NVLEIRNSQFDGPTLAPTLSLPWWSSPSFMTTHFSYPPPASVT 546

Query: 565 LSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQVDNGSSEVRYH 624
           LS V+RI   +         AA++  GK    SG +A+ FH+   +  Q    SS    +
Sbjct: 547 LSVVSRIKCNN------FFHAASSVVGKPTFPSGCLAAVFHQ---SVPQESQSSSPALDY 606

Query: 625 ILVFSPSGSMIQYAL--RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNR 684
           +LV++PSG ++QY L   +G D      R+     L SE + R+ VE +Q W++ ++ + 
Sbjct: 607 LLVYTPSGHVVQYKLIPSLGGDQAESNTRNGATSGLTSEEELRVKVEPVQCWDVCRRADW 666

Query: 685 RSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHLYISEAELQM 744
             ++ +I          C   Y    N    ++   +  ++K    E++H+Y++ AE+ +
Sbjct: 667 PEREENI----------CGLTYGGRKNAELTVDTSDSEDQTKPL--EKHHVYLANAEVLI 726

Query: 745 HAARTPLWTRPEIYFQVM---ARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDY 789
           ++ R P+W   EI F  M     DG  ++     GE +I ++    ++ R KDL+PV+D 
Sbjct: 727 NSGRKPIWQNSEISFYPMYPPDSDGKNLNSHQGGGETEIGKVSANEVDIRRKDLLPVYDN 743

BLAST of Chy4G077320 vs. ExPASy Swiss-Prot
Match: Q8CCN5 (BCAS3 microtubule associated cell migration factor OS=Mus musculus OX=10090 GN=Bcas3 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 4.2e-34
Identity = 116/419 (27.68%), Postives = 193/419 (46.06%), Query Frame = 0

Query: 98  FDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRS 157
           F +L    N    ++++G   G QVW +  +   QEL     G V   ++LP   L  + 
Sbjct: 79  FHELHSTGNEPPLLVMIGYSDGMQVWGIPISGEAQELFSVRHGPVRAARILPAPQLGAQK 138

Query: 158 GDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFMPTF--VRFYSL 217
            D FAE RPL+             +   + SSG                P +  V  YSL
Sbjct: 139 CDNFAEKRPLL------------GVCKSIGSSGTT--------------PPYCCVDLYSL 198

Query: 218 KSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNPVVSGFPGSG 277
           ++   V  ++F++ +Y + C+  ++ + L  +I    + T  K+       V S +P  G
Sbjct: 199 RTGEMVKSIQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFF-----VTSCYPCPG 258

Query: 278 GGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLVAHYAK---E 337
             M   P+ALG RWLAY+ + ++  +  R                + G  +  Y      
Sbjct: 259 PNM--NPIALGSRWLAYAENKLIRCHQSR--------------GGACGDNIQSYTATVLS 318

Query: 338 SSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQG---LKGIGTLNGHMADADS 397
           ++K L +G+  +G K + +L+      + E + +L   ++    + GI T    + D ++
Sbjct: 319 AAKTLKSGLTMVG-KVVTQLTGTLPSGVTEDDVALHCNSRRSPLVPGIIT----VIDTET 378

Query: 398 IG---MAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSC 457
           +G   + + +D  S  ++  F AH+ P+  + F+ SG +LVT    GH  +VF+I+    
Sbjct: 379 VGEGQVLVSEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQILTHPW 438

Query: 458 SKSSISGTAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQ 506
           S S       +  HLY L RG T A +QDI FS+D  W+++S+ RGTSH+F INP GGQ
Sbjct: 439 SSSQ-----CAVHHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQ 440

BLAST of Chy4G077320 vs. ExPASy Swiss-Prot
Match: Q9H6U6 (BCAS3 microtubule associated cell migration factor OS=Homo sapiens OX=9606 GN=BCAS3 PE=1 SV=3)

HSP 1 Score: 147.9 bits (372), Expect = 5.5e-34
Identity = 111/406 (27.34%), Postives = 190/406 (46.80%), Query Frame = 0

Query: 111 VLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVL 170
           ++++G   G QVW +  +   QEL     G +   ++LP      +  D FAE RPL+  
Sbjct: 92  LIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAEKRPLL-- 151

Query: 171 SAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFMPTF--VRFYSLKSQTYVHELKFRS 230
                      +   + SSG +              P +  V  YSL++   V  ++F++
Sbjct: 152 ----------GVCKSIGSSGTS--------------PPYCCVDLYSLRTGEMVKSIQFKT 211

Query: 231 AVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPR 290
            +Y + C+  ++ + L  +I    + T  K+       V S +P  G  M   P+ALG R
Sbjct: 212 PIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFF-----VTSCYPCPGPNM--NPIALGSR 271

Query: 291 WLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLVAHYAK---ESSKHLAAGIVTLG 350
           WLAY+ + ++  +  R                + G  +  Y      ++K L +G+  +G
Sbjct: 272 WLAYAENKLIRCHQSR--------------GGACGDNIQSYTATVISAAKTLKSGLTMVG 331

Query: 351 DKGIKKLSRYYSELLPESNNSLQSGAQG---LKGIGTLNGHMADADSIG---MAIVKDII 410
            K + +L+      + E + ++ S ++    + GI T    + D +++G   + + +D  
Sbjct: 332 -KVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIIT----VIDTETVGEGQVLVSEDSD 391

Query: 411 SKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYS 470
           S  ++  F AH+ P+  + F+ SG +LVT    GH  +VF+I+    S S       +  
Sbjct: 392 SDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQILTHPWSSSQ-----CAVH 440

Query: 471 HLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQ 506
           HLY L RG T A +QDI FS+D  W+++S+ RGTSH+F INP GGQ
Sbjct: 452 HLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQ 440

BLAST of Chy4G077320 vs. ExPASy TrEMBL
Match: A0A0A0LD33 (BCAS3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G415110 PE=4 SV=1)

HSP 1 Score: 1728.8 bits (4476), Expect = 0.0e+00
Identity = 887/907 (97.79%), Postives = 898/907 (99.01%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM
Sbjct: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           KIVSAGASTVARSAASVASSLVDKDDEANA QVNWAGFDKLEW DNVIRQVLLLGIRSGF
Sbjct: 61  KIVSAGASTVARSAASVASSLVDKDDEANASQVNWAGFDKLEWDDNVIRQVLLLGIRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           QVWDVEEANNVQELVCRYDGSVSYMQVLPRLI SMRSGDKFAESRPL+VLSAYGSIAANF
Sbjct: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLIPSMRSGDKFAESRPLIVLSAYGSIAANF 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           NIQD+LASSGNA IPKSQE+VDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV
Sbjct: 181 NIQDRLASSGNATIPKSQEVVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLATQIHCI+ATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS
Sbjct: 241 AISLATQIHCINATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL
Sbjct: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD
Sbjct: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSGTILVTASVQGHSINVFKIMPSSCSKSSIS TAASYSHLYRLQRGFTNAVIQDISFSY
Sbjct: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISSTAASYSHLYRLQRGFTNAVIQDISFSY 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIARNGEPVVPARQTVRRVDSGLHMP-K 540
           DSNWIMISSSRGTSHLFAINP+GGQVNFPSA IIARNG PVVPARQTVRRVDSGLHMP K
Sbjct: 481 DSNWIMISSSRGTSHLFAINPSGGQVNFPSADIIARNGGPVVPARQTVRRVDSGLHMPSK 540

Query: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQ 600
           QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTV 
Sbjct: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVH 600

Query: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK 660
           VDNGSSEVRYHILVFSPSGSMIQYALRVGLDST+VLPRSSTALELVSELD+RLVVEAIQK
Sbjct: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTVVLPRSSTALELVSELDSRLVVEAIQK 660

Query: 661 WNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHL 720
           WNISQKQNRRSQDNSIDIYGD+GGFNCNKNYCEEMNGNPVLEAGGNIFK+KACREEQYHL
Sbjct: 661 WNISQKQNRRSQDNSIDIYGDNGGFNCNKNYCEEMNGNPVLEAGGNIFKTKACREEQYHL 720

Query: 721 YISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780
           YISEAELQMHAARTPLWT+PEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL
Sbjct: 721 YISEAELQMHAARTPLWTKPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780

Query: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGREVTG 840
           VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSV+GNGREVTG
Sbjct: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVVGNGREVTG 840

Query: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 900
           RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE
Sbjct: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 900

Query: 901 DLGDEFE 907
           DLGDEFE
Sbjct: 901 DLGDEFE 907

BLAST of Chy4G077320 vs. ExPASy TrEMBL
Match: A0A1S3BZN8 (LOW QUALITY PROTEIN: autophagy-related protein 18f OS=Cucumis melo OX=3656 GN=LOC103494953 PE=4 SV=1)

HSP 1 Score: 1672.1 bits (4329), Expect = 0.0e+00
Identity = 865/907 (95.37%), Postives = 880/907 (97.02%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM
Sbjct: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           KIVSAGAS VARSAASVASSLVDKDDEANAFQVNWAGFDKLEW DNVIRQVLLLG RSGF
Sbjct: 61  KIVSAGASNVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWDDNVIRQVLLLGFRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           QVWDVEEANNVQ+LVCRYDGSVSYMQVLPR I SMRSGDKFAESRPL+VLSAYGSIAAN 
Sbjct: 121 QVWDVEEANNVQDLVCRYDGSVSYMQVLPRPIPSMRSGDKFAESRPLLVLSAYGSIAANL 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           NIQD+LASSGN AIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV
Sbjct: 181 NIQDRLASSGNVAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLATQIHCI+ATTLEKEHIILTNPVVSGFPGSGGG+GYGPLALGPRWLAYSGSPILLS
Sbjct: 241 AISLATQIHCINATTLEKEHIILTNPVVSGFPGSGGGIGYGPLALGPRWLAYSGSPILLS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRVVPQHLKPSASFSH SSNGSLVAHYAKESSK LAAGIVTLGDKGIKKLSRYYSELL
Sbjct: 301 NTGRVVPQHLKPSASFSH-SSNGSLVAHYAKESSKQLAAGIVTLGDKGIKKLSRYYSELL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PESNNSLQSGAQGLKGIGTLNGH+ADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD
Sbjct: 361 PESNNSLQSGAQGLKGIGTLNGHVADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSGTILVTASVQGHSINVFKIMPSS SKSS+SG AASYSHLYRLQRGFTNAVIQDISFSY
Sbjct: 421 PSGTILVTASVQGHSINVFKIMPSSSSKSSVSGAAASYSHLYRLQRGFTNAVIQDISFSY 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIARNGEPVVPARQTVRRVDSGLHMP-K 540
           DSNWIMISSSRGTSHLFAINPAGGQVNFPSA IIARNG PVVP+RQTVRRVDSGLHMP K
Sbjct: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSADIIARNGGPVVPSRQTVRRVDSGLHMPSK 540

Query: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQ 600
           QNQ +TGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGN V 
Sbjct: 541 QNQGSTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNAVH 600

Query: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK 660
           VDNGSSEVRYHIL FSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK
Sbjct: 601 VDNGSSEVRYHILXFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK 660

Query: 661 WNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHL 720
           WNISQKQNRRSQDNSIDIYGD+GGFNCNKNYCEEMNGNPVLEAGGNIFK+KACREE+YHL
Sbjct: 661 WNISQKQNRRSQDNSIDIYGDNGGFNCNKNYCEEMNGNPVLEAGGNIFKAKACREERYHL 720

Query: 721 YISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780
           YISEAELQMHAARTPLWT+PEIYFQVM RDGVKIDEIDHPGELDIERI TRM+EARSKDL
Sbjct: 721 YISEAELQMHAARTPLWTKPEIYFQVMGRDGVKIDEIDHPGELDIERITTRMVEARSKDL 780

Query: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGREVTG 840
           VPVFDYLQSSKISQPRYLNS+SDQLL QQKSGQFENGRQSWRSAANP DSV GNGREVTG
Sbjct: 781 VPVFDYLQSSKISQPRYLNSSSDQLLQQQKSGQFENGRQSWRSAANPQDSVFGNGREVTG 840

Query: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 900
            HGYK VTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKS L VENHFE
Sbjct: 841 GHGYKVVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSVLEVENHFE 900

Query: 901 DLGDEFE 907
           DLGDEFE
Sbjct: 901 DLGDEFE 906

BLAST of Chy4G077320 vs. ExPASy TrEMBL
Match: A0A5A7TTR5 (Autophagy-related protein 18f OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1251G00670 PE=4 SV=1)

HSP 1 Score: 1630.5 bits (4221), Expect = 0.0e+00
Identity = 840/881 (95.35%), Postives = 855/881 (97.05%), Query Frame = 0

Query: 27  MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 86
           MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGAS VARSAASVASSLVDKDD
Sbjct: 1   MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASNVARSAASVASSLVDKDD 60

Query: 87  EANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQ 146
           EANAFQVNWAGFDKLEW DNVIRQVLLLG RSGFQVWDVEEANNVQ+LVCRYDGSVSYMQ
Sbjct: 61  EANAFQVNWAGFDKLEWDDNVIRQVLLLGFRSGFQVWDVEEANNVQDLVCRYDGSVSYMQ 120

Query: 147 VLPRLILSMRSGDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFM 206
           VLPR I SMRSGDKFAESRPL+VLSAYGSIAAN NIQD+LASSGN AIPKSQELVDGNFM
Sbjct: 121 VLPRPIPSMRSGDKFAESRPLLVLSAYGSIAANLNIQDRLASSGNVAIPKSQELVDGNFM 180

Query: 207 PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNP 266
           PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCI+ATTLEKEHIILTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCINATTLEKEHIILTNP 240

Query: 267 VVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLV 326
           VVSGFPGSGGG+GYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSH SSNGSLV
Sbjct: 241 VVSGFPGSGGGIGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSH-SSNGSLV 300

Query: 327 AHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMAD 386
           AHYAKESSK LAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGH+AD
Sbjct: 301 AHYAKESSKQLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHVAD 360

Query: 387 ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSC 446
           ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSS 
Sbjct: 361 ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSS 420

Query: 447 SKSSISGTAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQV 506
           SKSS+SG AASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQV
Sbjct: 421 SKSSVSGAAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQV 480

Query: 507 NFPSAVIIARNGEPVVPARQTVRRVDSGLHMP-KQNQCTTGSPLTLSAVTRIHHGSNGWR 566
           NFPSA IIARNG PVVP+RQTVRRVDSGLHMP KQNQ +TGSPLTLSAVTRIHHGSNGWR
Sbjct: 481 NFPSADIIARNGGPVVPSRQTVRRVDSGLHMPSKQNQGSTGSPLTLSAVTRIHHGSNGWR 540

Query: 567 GTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQVDNGSSEVRYHILVFSPSGSMIQYAL 626
           GTVSSAAAAATGKMGIVSGAIASAFHECKGN V VDNGSSEVRYHILVFSPSGSMIQYAL
Sbjct: 541 GTVSSAAAAATGKMGIVSGAIASAFHECKGNAVHVDNGSSEVRYHILVFSPSGSMIQYAL 600

Query: 627 RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRRSQDNSIDIYGDDGGFN 686
           RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRRSQDNSIDIYGD+GGFN
Sbjct: 601 RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRRSQDNSIDIYGDNGGFN 660

Query: 687 CNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHLYISEAELQMHAARTPLWTRPEIYFQV 746
           CNKNYCEEMNGNPVLEAGGNIFK+KACREE+YHLYISEAELQMHAARTPLWT+PEIYFQV
Sbjct: 661 CNKNYCEEMNGNPVLEAGGNIFKAKACREERYHLYISEAELQMHAARTPLWTKPEIYFQV 720

Query: 747 MARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDYLQSSKISQPRYLNSNSDQLL 806
           M RDGVKIDEIDHPGELDIERI TRM+EARSKDLVPVFDYLQSSKISQPRYLNS+SDQLL
Sbjct: 721 MGRDGVKIDEIDHPGELDIERITTRMVEARSKDLVPVFDYLQSSKISQPRYLNSSSDQLL 780

Query: 807 LQQKSGQFENGRQSWRSAANPHDSVIGNGREVTGRHGYKRVTETKGIVNEPECPRSQTQF 866
            QQKSGQFENGRQSWRSAANP DSV GNGREVTG HGYK VTETKGIVNEPECPRSQTQF
Sbjct: 781 QQQKSGQFENGRQSWRSAANPQDSVFGNGREVTGGHGYKVVTETKGIVNEPECPRSQTQF 840

Query: 867 SNVNSCTESISMESQPKSVNNHHKSGLRVENHFEDLGDEFE 907
           SNVNSCTESISMESQPKSVNNHHKS L VENHFEDLGDEFE
Sbjct: 841 SNVNSCTESISMESQPKSVNNHHKSVLEVENHFEDLGDEFE 880

BLAST of Chy4G077320 vs. ExPASy TrEMBL
Match: A0A6J1FA02 (autophagy-related protein 18f-like OS=Cucurbita moschata OX=3662 GN=LOC111443454 PE=4 SV=1)

HSP 1 Score: 1414.1 bits (3659), Expect = 0.0e+00
Identity = 745/904 (82.41%), Postives = 797/904 (88.16%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MI+V+  ELVILVFWVL +  TLILGMRNDG LKQQG+VARSGRAH F+S+SFRAFSSYM
Sbjct: 1   MIRVESEELVILVFWVLGVLFTLILGMRNDGALKQQGSVARSGRAHGFISSSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           K VS+GASTVARSAASVASSLVDKD EA   QVNWAGFDKLE  DNVIR+VLLLG RSGF
Sbjct: 61  KNVSSGASTVARSAASVASSLVDKDGEAKGSQVNWAGFDKLERDDNVIRRVLLLGFRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           Q+WDVEEANNVQ LV RYDGSV YMQVLPR ILSMRSGDKFAESRPL+VLSA GSIAA F
Sbjct: 121 QIWDVEEANNVQVLVSRYDGSVLYMQVLPRPILSMRSGDKFAESRPLLVLSASGSIAAAF 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           NIQDQLA+SGNAAI K QELVDGNFMPTFVRFYSLKSQTYV ELKFRS VYSV+CSPLVV
Sbjct: 181 NIQDQLATSGNAAISKGQELVDGNFMPTFVRFYSLKSQTYVDELKFRSVVYSVRCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLATQIHCI+ATTLEKEHIILTNPVVSGFPGSGGG+GYGPLA+GPRWLAYSGSPI +S
Sbjct: 241 AISLATQIHCINATTLEKEHIILTNPVVSGFPGSGGGIGYGPLAVGPRWLAYSGSPITVS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRV+PQHLKPSASFSHSSSNGSLVAHYAKESSK LAAGIVTLGDKGIKKLSRYYS+LL
Sbjct: 301 NTGRVIPQHLKPSASFSHSSSNGSLVAHYAKESSKQLAAGIVTLGDKGIKKLSRYYSDLL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PES NSLQSG Q   GIG LNGH AD DS+GM IVKDI+SK +ITQFKAHKSPISALCF+
Sbjct: 361 PESYNSLQSGTQ---GIGALNGHAADTDSVGMVIVKDIVSKVIITQFKAHKSPISALCFN 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSG ILVTASVQGHS+NVFKIMPSSCS SS     ASYSHLYRLQRGFTNAVIQDISFSY
Sbjct: 421 PSGIILVTASVQGHSVNVFKIMPSSCSDSS-----ASYSHLYRLQRGFTNAVIQDISFSY 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSAVI--IARNGEPVVPARQTVRRVDSGLHM- 540
           DSNWIMISSSRGTSHLFAINP+GGQVN PSA I  I RNG PVVPARQ VR+VDSG  + 
Sbjct: 481 DSNWIMISSSRGTSHLFAINPSGGQVNIPSADISSITRNGSPVVPARQIVRQVDSGSRVS 540

Query: 541 PKQNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNT 600
            KQ+QC TGSP TLS +TR+HHGSNGWRGTVSSAAAAATG++GIV GAIAS FHECKGN 
Sbjct: 541 SKQSQCATGSPPTLSPITRLHHGSNGWRGTVSSAAAAATGRVGIVLGAIASTFHECKGNA 600

Query: 601 VQVDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAI 660
           + VDNGSSE RY ILVFSPSG+MIQYALRVGLDST+VLPRSSTALEL  ELDARLVVE I
Sbjct: 601 LHVDNGSSEARYDILVFSPSGNMIQYALRVGLDSTLVLPRSSTALELAPELDARLVVEPI 660

Query: 661 QKWNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNP-VLEAGGNIFKSKACREEQ 720
           QKWNISQK N R+QDNSIDIYGD GGF+C KNYCEEMNGN  VLEA G + K++ CREEQ
Sbjct: 661 QKWNISQKHNCRAQDNSIDIYGDSGGFDCKKNYCEEMNGNSIVLEASGTVLKAETCREEQ 720

Query: 721 YHLYISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARS 780
           YHLYISEAELQMHAA TPLWT+PEIYFQVMARDGV+ DEID  GEL+IERIPTRMIEARS
Sbjct: 721 YHLYISEAELQMHAALTPLWTKPEIYFQVMARDGVERDEIDRYGELEIERIPTRMIEARS 780

Query: 781 KDLVPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGRE 840
           KDL+PVFD+LQ  K  Q R LNS+SD      KS + ENGRQSWR+   PH SVIGNG E
Sbjct: 781 KDLIPVFDHLQPPKFPQTRLLNSSSD-----PKSIRSENGRQSWRNVTIPHGSVIGNGPE 840

Query: 841 VTGRHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVEN 900
            TGRHGYK VTETKG VNE ECPRSQTQ ++VN+CTES+SMESQPKSVNN HKSGL VEN
Sbjct: 841 GTGRHGYKMVTETKGTVNEYECPRSQTQLNDVNNCTESLSMESQPKSVNN-HKSGLGVEN 890

BLAST of Chy4G077320 vs. ExPASy TrEMBL
Match: A0A6J1CE59 (autophagy-related protein 18f OS=Momordica charantia OX=3673 GN=LOC111010412 PE=4 SV=1)

HSP 1 Score: 1406.3 bits (3639), Expect = 0.0e+00
Identity = 741/909 (81.52%), Postives = 802/909 (88.23%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MI+V+ GELVILVFW L I LTLILGMRNDG LKQQGTVARSGRAHEF+S+SFRAFSSYM
Sbjct: 1   MIRVEFGELVILVFWALGILLTLILGMRNDGALKQQGTVARSGRAHEFISSSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           KIVSAGASTVARSAASVASSLVDKD+EAN FQVNWAGFDKLEW DNVIR+VLLL  RSGF
Sbjct: 61  KIVSAGASTVARSAASVASSLVDKDEEANGFQVNWAGFDKLEWDDNVIRRVLLLSFRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           QVWDVEEAN+VQ+LV RYDGSVS MQVLPR I SMRSGDKFAESRPL+VLSAYGSIAA  
Sbjct: 121 QVWDVEEANSVQDLVSRYDGSVSNMQVLPRPIPSMRSGDKFAESRPLLVLSAYGSIAAAC 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           +IQD+LA+SGNAAIPK QELVDG+FMPTFVRFYSLKSQTY HELKFRS VYSV+CSPLVV
Sbjct: 181 DIQDRLATSGNAAIPKCQELVDGSFMPTFVRFYSLKSQTYFHELKFRSVVYSVRCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLA QIHCI+ATTLEKEHIILTNPVVSGFPGSGGG+GYGPLA+GPRWLAYSGSPI++S
Sbjct: 241 AISLANQIHCINATTLEKEHIILTNPVVSGFPGSGGGIGYGPLAVGPRWLAYSGSPIMVS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRV PQHLK SASFSHSSSNGSLVAHYAKESSK +AAGIVTLGDKGIKKLSRYYS+LL
Sbjct: 301 NTGRVSPQHLKHSASFSHSSSNGSLVAHYAKESSKQIAAGIVTLGDKGIKKLSRYYSDLL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PESNNSLQSGAQGLKGIG LNG + DADSIGM IVKDIISK +ITQFKAHKSPISALCFD
Sbjct: 361 PESNNSLQSGAQGLKGIGALNGQVTDADSIGMVIVKDIISKVIITQFKAHKSPISALCFD 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSGTILVTASV GH+INVFKIMPSS   SS SGT AS  HLYRLQRGFTNAVIQDISFSY
Sbjct: 421 PSGTILVTASVHGHNINVFKIMPSSSLNSSASGTGASSIHLYRLQRGFTNAVIQDISFSY 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPS--AVIIARNGEPVVPARQTVRRVDSGLHMP 540
           DSNWIMISSSRGTSHLFAINPAGGQVNFPS  A  I RNG   VPARQT+RRVDSGLHMP
Sbjct: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSADANFIIRNGGSGVPARQTLRRVDSGLHMP 540

Query: 541 -KQNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNT 600
            KQ+ C TG P+TL AV+RIHHGSNGWRGTVSSAAAAATG+MG VSGAIASAFH+C+ N 
Sbjct: 541 SKQDPCATGVPVTLFAVSRIHHGSNGWRGTVSSAAAAATGRMGTVSGAIASAFHDCRVNA 600

Query: 601 VQVDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAI 660
           + +DNGSSE RYHILVFSP+GSMIQYALRVGLDST VLPRSSTALE V E D RLVVEAI
Sbjct: 601 LHIDNGSSEARYHILVFSPTGSMIQYALRVGLDSTEVLPRSSTALESVPESDGRLVVEAI 660

Query: 661 QKWNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPV-LEAGGNIFKSKACREEQ 720
           QKWNISQKQNR++QDNSIDIYGD+GG  CNKNYC E+NGNP+ L A   + K+KACREE 
Sbjct: 661 QKWNISQKQNRKAQDNSIDIYGDNGGLECNKNYCGEINGNPIYLGASDTVLKAKACREEL 720

Query: 721 YHLYISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARS 780
           +H YISEAELQM AAR+PLWT+PEIYFQ MARDGVK DEI   GE++IERIPTRMIEARS
Sbjct: 721 HHFYISEAELQMQAARSPLWTKPEIYFQAMARDGVKGDEIGSLGEIEIERIPTRMIEARS 780

Query: 781 KDLVPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGRE 840
           KDLVPVFDYLQ  KISQ RYL++NS+ LL Q+ S   ENGRQS  + A PHDS+IGN + 
Sbjct: 781 KDLVPVFDYLQLPKISQTRYLDNNSEPLLHQKLSVLVENGRQSLGNNAGPHDSIIGNEQA 840

Query: 841 VTGRHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVEN 900
            TGRHG K VTE+KGIVN+ E P S+TQ +NVN+CTES+ MES  KSVNN  +SG +V N
Sbjct: 841 GTGRHGCKMVTESKGIVND-ESPNSETQLNNVNNCTESLRMESPHKSVNN-QESGPKVVN 900

Query: 901 HFEDLGDEF 906
            FED  DEF
Sbjct: 901 LFEDTDDEF 907

BLAST of Chy4G077320 vs. NCBI nr
Match: XP_004151961.2 (LOW QUALITY PROTEIN: autophagy-related protein 18f [Cucumis sativus])

HSP 1 Score: 1714 bits (4440), Expect = 0.0
Identity = 886/907 (97.68%), Postives = 897/907 (98.90%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM
Sbjct: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           KIVSAGASTVARSAASVASSLVDKDDEANA QVNWAGFDKLEW DNVIRQVLLLGIRSGF
Sbjct: 61  KIVSAGASTVARSAASVASSLVDKDDEANASQVNWAGFDKLEWDDNVIRQVLLLGIRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           QVWDVEEANNVQELVCRYDGSVSYMQVLPRLI SMRSGDKFAESRPL+VLSAYGSIAANF
Sbjct: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLIPSMRSGDKFAESRPLIVLSAYGSIAANF 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           NIQD+LASSGNA IPKSQE+VDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV
Sbjct: 181 NIQDRLASSGNATIPKSQEVVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLATQIHCI+ATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS
Sbjct: 241 AISLATQIHCINATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL
Sbjct: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD
Sbjct: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSGTILVTASVQGHSINVFKIMPSSCSKSSIS TAASYSHLYRLQRGFTNAVIQDISFSY
Sbjct: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISSTAASYSHLYRLQRGFTNAVIQDISFSY 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIARNGEPVVPARQTVRRVDSGLHMP-K 540
           DSNWIMISSSRGTSHLFAINP+ GQVNFPSA IIARNG PVVPARQTVRRVDSGLHMP K
Sbjct: 481 DSNWIMISSSRGTSHLFAINPSXGQVNFPSADIIARNGGPVVPARQTVRRVDSGLHMPSK 540

Query: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQ 600
           QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTV 
Sbjct: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVH 600

Query: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK 660
           VDNGSSEVRYHILVFSPSGSMIQYALRVGLDST+VLPRSSTALELVSELD+RLVVEAIQK
Sbjct: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTVVLPRSSTALELVSELDSRLVVEAIQK 660

Query: 661 WNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHL 720
           WNISQKQNRRSQDNSIDIYGD+GGFNCNKNYCEEMNGNPVLEAGGNIFK+KACREEQYHL
Sbjct: 661 WNISQKQNRRSQDNSIDIYGDNGGFNCNKNYCEEMNGNPVLEAGGNIFKTKACREEQYHL 720

Query: 721 YISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780
           YISEAELQMHAARTPLWT+PEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL
Sbjct: 721 YISEAELQMHAARTPLWTKPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780

Query: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGREVTG 840
           VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSV+GNGREVTG
Sbjct: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVVGNGREVTG 840

Query: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 900
           RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE
Sbjct: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 900

Query: 901 DLGDEFE 906
           DLGDEFE
Sbjct: 901 DLGDEFE 907

BLAST of Chy4G077320 vs. NCBI nr
Match: XP_008454562.1 (PREDICTED: LOW QUALITY PROTEIN: autophagy-related protein 18f [Cucumis melo])

HSP 1 Score: 1660 bits (4300), Expect = 0.0
Identity = 865/907 (95.37%), Postives = 880/907 (97.02%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM
Sbjct: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           KIVSAGAS VARSAASVASSLVDKDDEANAFQVNWAGFDKLEW DNVIRQVLLLG RSGF
Sbjct: 61  KIVSAGASNVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWDDNVIRQVLLLGFRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           QVWDVEEANNVQ+LVCRYDGSVSYMQVLPR I SMRSGDKFAESRPL+VLSAYGSIAAN 
Sbjct: 121 QVWDVEEANNVQDLVCRYDGSVSYMQVLPRPIPSMRSGDKFAESRPLLVLSAYGSIAANL 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           NIQD+LASSGN AIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV
Sbjct: 181 NIQDRLASSGNVAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLATQIHCI+ATTLEKEHIILTNPVVSGFPGSGGG+GYGPLALGPRWLAYSGSPILLS
Sbjct: 241 AISLATQIHCINATTLEKEHIILTNPVVSGFPGSGGGIGYGPLALGPRWLAYSGSPILLS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRVVPQHLKPSASFSHSS NGSLVAHYAKESSK LAAGIVTLGDKGIKKLSRYYSELL
Sbjct: 301 NTGRVVPQHLKPSASFSHSS-NGSLVAHYAKESSKQLAAGIVTLGDKGIKKLSRYYSELL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PESNNSLQSGAQGLKGIGTLNGH+ADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD
Sbjct: 361 PESNNSLQSGAQGLKGIGTLNGHVADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSGTILVTASVQGHSINVFKIMPSS SKSS+SG AASYSHLYRLQRGFTNAVIQDISFSY
Sbjct: 421 PSGTILVTASVQGHSINVFKIMPSSSSKSSVSGAAASYSHLYRLQRGFTNAVIQDISFSY 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIARNGEPVVPARQTVRRVDSGLHMP-K 540
           DSNWIMISSSRGTSHLFAINPAGGQVNFPSA IIARNG PVVP+RQTVRRVDSGLHMP K
Sbjct: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSADIIARNGGPVVPSRQTVRRVDSGLHMPSK 540

Query: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQ 600
           QNQ +TGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGN V 
Sbjct: 541 QNQGSTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNAVH 600

Query: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK 660
           VDNGSSEVRYHIL FSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK
Sbjct: 601 VDNGSSEVRYHILXFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK 660

Query: 661 WNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHL 720
           WNISQKQNRRSQDNSIDIYGD+GGFNCNKNYCEEMNGNPVLEAGGNIFK+KACREE+YHL
Sbjct: 661 WNISQKQNRRSQDNSIDIYGDNGGFNCNKNYCEEMNGNPVLEAGGNIFKAKACREERYHL 720

Query: 721 YISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780
           YISEAELQMHAARTPLWT+PEIYFQVM RDGVKIDEIDHPGELDIERI TRM+EARSKDL
Sbjct: 721 YISEAELQMHAARTPLWTKPEIYFQVMGRDGVKIDEIDHPGELDIERITTRMVEARSKDL 780

Query: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGREVTG 840
           VPVFDYLQSSKISQPRYLNS+SDQLL QQKSGQFENGRQSWRSAANP DSV GNGREVTG
Sbjct: 781 VPVFDYLQSSKISQPRYLNSSSDQLLQQQKSGQFENGRQSWRSAANPQDSVFGNGREVTG 840

Query: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 900
            HGYK VTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKS L VENHFE
Sbjct: 841 GHGYKVVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSVLEVENHFE 900

Query: 901 DLGDEFE 906
           DLGDEFE
Sbjct: 901 DLGDEFE 906

BLAST of Chy4G077320 vs. NCBI nr
Match: KAE8650685.1 (hypothetical protein Csa_011154 [Cucumis sativus])

HSP 1 Score: 1633 bits (4228), Expect = 0.0
Identity = 854/907 (94.16%), Postives = 864/907 (95.26%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM
Sbjct: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           KIVSAGASTVARSAASVASSLVDKDDEANA QVNWAGFDKLEW DNVIRQVLLLGIRSGF
Sbjct: 61  KIVSAGASTVARSAASVASSLVDKDDEANASQVNWAGFDKLEWDDNVIRQVLLLGIRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           QVWDVEEANNVQELVCRYDGSVSYMQVLPRLI SMRSGDKFAESRPL+VLSAYGSIAANF
Sbjct: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLIPSMRSGDKFAESRPLIVLSAYGSIAANF 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           NIQD+LASSGNA IPKSQE+VDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV
Sbjct: 181 NIQDRLASSGNATIPKSQEVVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLATQIHCI+ATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS
Sbjct: 241 AISLATQIHCINATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL
Sbjct: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD
Sbjct: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSGTILVTASVQGHSINVFKIMPSSCSKSSIS TAASYSHLYRLQRGFTNAV        
Sbjct: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISSTAASYSHLYRLQRGFTNAV-------- 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIARNGEPVVPARQTVRRVDSGLHMP-K 540
                                     NFPSA IIARNG PVVPARQTVRRVDSGLHMP K
Sbjct: 481 --------------------------NFPSADIIARNGGPVVPARQTVRRVDSGLHMPSK 540

Query: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQ 600
           QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTV 
Sbjct: 541 QNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVH 600

Query: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAIQK 660
           VDNGSSEVRYHILVFSPSGSMIQYALRVGLDST+VLPRSSTALELVSELD+RLVVEAIQK
Sbjct: 601 VDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTVVLPRSSTALELVSELDSRLVVEAIQK 660

Query: 661 WNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHL 720
           WNISQKQNRRSQDNSIDIYGD+GGFNCNKNYCEEMNGNPVLEAGGNIFK+KACREEQYHL
Sbjct: 661 WNISQKQNRRSQDNSIDIYGDNGGFNCNKNYCEEMNGNPVLEAGGNIFKTKACREEQYHL 720

Query: 721 YISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780
           YISEAELQMHAARTPLWT+PEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL
Sbjct: 721 YISEAELQMHAARTPLWTKPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDL 780

Query: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGREVTG 840
           VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSV+GNGREVTG
Sbjct: 781 VPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVVGNGREVTG 840

Query: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 900
           RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE
Sbjct: 841 RHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENHFE 873

Query: 901 DLGDEFE 906
           DLGDEFE
Sbjct: 901 DLGDEFE 873

BLAST of Chy4G077320 vs. NCBI nr
Match: KAA0046454.1 (autophagy-related protein 18f [Cucumis melo var. makuwa] >TYJ97759.1 autophagy-related protein 18f [Cucumis melo var. makuwa])

HSP 1 Score: 1620 bits (4195), Expect = 0.0
Identity = 840/881 (95.35%), Postives = 855/881 (97.05%), Query Frame = 0

Query: 27  MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 86
           MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGAS VARSAASVASSLVDKDD
Sbjct: 1   MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASNVARSAASVASSLVDKDD 60

Query: 87  EANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQ 146
           EANAFQVNWAGFDKLEW DNVIRQVLLLG RSGFQVWDVEEANNVQ+LVCRYDGSVSYMQ
Sbjct: 61  EANAFQVNWAGFDKLEWDDNVIRQVLLLGFRSGFQVWDVEEANNVQDLVCRYDGSVSYMQ 120

Query: 147 VLPRLILSMRSGDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFM 206
           VLPR I SMRSGDKFAESRPL+VLSAYGSIAAN NIQD+LASSGN AIPKSQELVDGNFM
Sbjct: 121 VLPRPIPSMRSGDKFAESRPLLVLSAYGSIAANLNIQDRLASSGNVAIPKSQELVDGNFM 180

Query: 207 PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNP 266
           PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCI+ATTLEKEHIILTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCINATTLEKEHIILTNP 240

Query: 267 VVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLV 326
           VVSGFPGSGGG+GYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSS NGSLV
Sbjct: 241 VVSGFPGSGGGIGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSS-NGSLV 300

Query: 327 AHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMAD 386
           AHYAKESSK LAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGH+AD
Sbjct: 301 AHYAKESSKQLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHVAD 360

Query: 387 ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSC 446
           ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSS 
Sbjct: 361 ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSS 420

Query: 447 SKSSISGTAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQV 506
           SKSS+SG AASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQV
Sbjct: 421 SKSSVSGAAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQV 480

Query: 507 NFPSAVIIARNGEPVVPARQTVRRVDSGLHMP-KQNQCTTGSPLTLSAVTRIHHGSNGWR 566
           NFPSA IIARNG PVVP+RQTVRRVDSGLHMP KQNQ +TGSPLTLSAVTRIHHGSNGWR
Sbjct: 481 NFPSADIIARNGGPVVPSRQTVRRVDSGLHMPSKQNQGSTGSPLTLSAVTRIHHGSNGWR 540

Query: 567 GTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQVDNGSSEVRYHILVFSPSGSMIQYAL 626
           GTVSSAAAAATGKMGIVSGAIASAFHECKGN V VDNGSSEVRYHILVFSPSGSMIQYAL
Sbjct: 541 GTVSSAAAAATGKMGIVSGAIASAFHECKGNAVHVDNGSSEVRYHILVFSPSGSMIQYAL 600

Query: 627 RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRRSQDNSIDIYGDDGGFN 686
           RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRRSQDNSIDIYGD+GGFN
Sbjct: 601 RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRRSQDNSIDIYGDNGGFN 660

Query: 687 CNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHLYISEAELQMHAARTPLWTRPEIYFQV 746
           CNKNYCEEMNGNPVLEAGGNIFK+KACREE+YHLYISEAELQMHAARTPLWT+PEIYFQV
Sbjct: 661 CNKNYCEEMNGNPVLEAGGNIFKAKACREERYHLYISEAELQMHAARTPLWTKPEIYFQV 720

Query: 747 MARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDYLQSSKISQPRYLNSNSDQLL 806
           M RDGVKIDEIDHPGELDIERI TRM+EARSKDLVPVFDYLQSSKISQPRYLNS+SDQLL
Sbjct: 721 MGRDGVKIDEIDHPGELDIERITTRMVEARSKDLVPVFDYLQSSKISQPRYLNSSSDQLL 780

Query: 807 LQQKSGQFENGRQSWRSAANPHDSVIGNGREVTGRHGYKRVTETKGIVNEPECPRSQTQF 866
            QQKSGQFENGRQSWRSAANP DSV GNGREVTG HGYK VTETKGIVNEPECPRSQTQF
Sbjct: 781 QQQKSGQFENGRQSWRSAANPQDSVFGNGREVTGGHGYKVVTETKGIVNEPECPRSQTQF 840

Query: 867 SNVNSCTESISMESQPKSVNNHHKSGLRVENHFEDLGDEFE 906
           SNVNSCTESISMESQPKSVNNHHKS L VENHFEDLGDEFE
Sbjct: 841 SNVNSCTESISMESQPKSVNNHHKSVLEVENHFEDLGDEFE 880

BLAST of Chy4G077320 vs. NCBI nr
Match: XP_038897831.1 (autophagy-related protein 18f [Benincasa hispida])

HSP 1 Score: 1563 bits (4047), Expect = 0.0
Identity = 809/909 (89.00%), Postives = 848/909 (93.29%), Query Frame = 0

Query: 1   MIKVDVGELVILVFWVLAIFLTLILGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYM 60
           MIKVDVGELVILVFWV +I LTL LGMRNDG LKQQGTVARSGRAHEFLS+SFRAFSSYM
Sbjct: 1   MIKVDVGELVILVFWVSSILLTLFLGMRNDGALKQQGTVARSGRAHEFLSSSFRAFSSYM 60

Query: 61  KIVSAGASTVARSAASVASSLVDKDDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGF 120
           KIVSAGASTVARSAASVASSLVDKDDEAN FQVNWAGFDKLEW DN IRQVLLLG RSGF
Sbjct: 61  KIVSAGASTVARSAASVASSLVDKDDEANGFQVNWAGFDKLEWDDNAIRQVLLLGFRSGF 120

Query: 121 QVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANF 180
           QVWDVEEANNVQ+LV RYDGSVSYMQVLPR I SMR GDKFAESRPL+VLSAYGSIAA F
Sbjct: 121 QVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMRPGDKFAESRPLLVLSAYGSIAAAF 180

Query: 181 NIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVV 240
           N+QD+LASSGNAAIPK QELVDGN MPTFVRFYSLKSQTYVHELKFRSAVYSV+CSPLVV
Sbjct: 181 NVQDRLASSGNAAIPKGQELVDGNLMPTFVRFYSLKSQTYVHELKFRSAVYSVRCSPLVV 240

Query: 241 AISLATQIHCISATTLEKEHIILTNPVVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLS 300
           AISLATQ+HCI+ATTLEKEHIILTNPVVSG PGSGGG+GYGPLA+GPRWLAYSG+ I++S
Sbjct: 241 AISLATQVHCINATTLEKEHIILTNPVVSGIPGSGGGIGYGPLAVGPRWLAYSGNLIMVS 300

Query: 301 NTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELL 360
           NTGRV+PQHLKPSASFSHS+SNGSLVAHYAKESSK LAAGIVTLGDKGIKKLSRYYSELL
Sbjct: 301 NTGRVIPQHLKPSASFSHSASNGSLVAHYAKESSKQLAAGIVTLGDKGIKKLSRYYSELL 360

Query: 361 PESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFD 420
           PESNNSLQSG QGLKGIG LNGH ADADS+GM IVKDIISK VITQFKAHKSPISALCFD
Sbjct: 361 PESNNSLQSGGQGLKGIGALNGHAADADSVGMVIVKDIISKVVITQFKAHKSPISALCFD 420

Query: 421 PSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGFTNAVIQDISFSY 480
           PSGTILVTASVQGHSINVFKIMPSS S SS S T+ASYSHLYRLQRGFTNAVIQDISFSY
Sbjct: 421 PSGTILVTASVQGHSINVFKIMPSSSSNSSESSTSASYSHLYRLQRGFTNAVIQDISFSY 480

Query: 481 DSNWIMISSSRGTSHLFAINPAGGQVNFPSAVI--IARNGEPVVPARQTVRRVDSGLHMP 540
           DSNWIM+SSSRGTSHLFAINPAGGQVNFPSA I  I RNG PVVPARQTVRRVD GLHMP
Sbjct: 481 DSNWIMVSSSRGTSHLFAINPAGGQVNFPSADISSITRNGGPVVPARQTVRRVDYGLHMP 540

Query: 541 -KQNQCTTGSPLTLSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNT 600
            KQNQC TGSPLTLSAVTRIHHG+NGWRGTVSSAAAAATG+MG VSGAIASAFHECKGN 
Sbjct: 541 SKQNQCATGSPLTLSAVTRIHHGNNGWRGTVSSAAAAATGRMGTVSGAIASAFHECKGNA 600

Query: 601 VQVDNGSSEVRYHILVFSPSGSMIQYALRVGLDSTIVLPRSSTALELVSELDARLVVEAI 660
           V VD+GSSEVRYHILVFSPSGSMIQYALRVGLDST VLPRSS ALELV ELDARLVVEAI
Sbjct: 601 VHVDSGSSEVRYHILVFSPSGSMIQYALRVGLDSTKVLPRSSMALELVPELDARLVVEAI 660

Query: 661 QKWNISQKQNRRSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQY 720
           QKWNISQKQNRR+QDNS+DIYGD+GGF+CNKNYCEEMN NPVLEA GN+FK+K CREEQY
Sbjct: 661 QKWNISQKQNRRAQDNSVDIYGDNGGFDCNKNYCEEMNENPVLEATGNVFKAKTCREEQY 720

Query: 721 HLYISEAELQMHAARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSK 780
           HLYISEAELQMH   TPLWT+ EIYFQVMA+DGV +DE +HPGEL+IERIPTRMIEARSK
Sbjct: 721 HLYISEAELQMHGTHTPLWTKSEIYFQVMAKDGVHVDEFNHPGELEIERIPTRMIEARSK 780

Query: 781 DLVPVFDYLQSSKISQPRYLNSNSDQLLLQQKSGQFENGRQSWRSAANPHDSVIGNGREV 840
           DLVPVFDYLQSSKISQPRYLNSNSDQLLL QKSGQFENGRQSWRS A+PH SV+G   EV
Sbjct: 781 DLVPVFDYLQSSKISQPRYLNSNSDQLLLHQKSGQFENGRQSWRSDASPHGSVVGK-PEV 840

Query: 841 TGRHGYKRVTETKGIVNEPECPRSQTQFSNVNSCTESISMESQPKSVNNHHKSGLRVENH 900
           T RHGYK VTETKGIVNE ECPRSQT  +NVN+CTES+SMESQPKSVNNHHKSGLR+ENH
Sbjct: 841 TDRHGYKMVTETKGIVNEYECPRSQTHLNNVNNCTESLSMESQPKSVNNHHKSGLRLENH 900

Query: 901 FEDLGDEFE 906
           FEDLGDEFE
Sbjct: 901 FEDLGDEFE 908

BLAST of Chy4G077320 vs. TAIR 10
Match: AT5G54730.1 (homolog of yeast autophagy 18 (ATG18) F )

HSP 1 Score: 490.3 bits (1261), Expect = 3.2e-138
Identity = 339/773 (43.86%), Postives = 453/773 (58.60%), Query Frame = 0

Query: 37  GTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDDEANAFQVNWA 96
           G V+RS R      +SFRA S+ +K++S+GASTVARSA S ASS V    E++  QV WA
Sbjct: 15  GVVSRSAR------SSFRALSNCLKVISSGASTVARSAVSAASSAV----ESHHDQVLWA 74

Query: 97  GFDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMR 156
           GFD L+  D   R+VLLL  +SGFQVWDVE+  NV  +V  +DG   +MQ+L   I S  
Sbjct: 75  GFDNLQKEDGDTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV 134

Query: 157 SGDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFMPTFVRFYSLK 216
             D+F +SRPL+ +          +   +  SS N   P S+ +      PT V  YSLK
Sbjct: 135 LDDRFYKSRPLLAVCG--------DYSSKKISSDN---PGSETVA----TPTNVYVYSLK 194

Query: 217 SQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNPVVSGFPGSGG 276
           SQ+YVH LKFR+ +YSV+C   +VA+  A QI C  A TLE ++ I+TN +V G      
Sbjct: 195 SQSYVHTLKFRATIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIVCG----SL 254

Query: 277 GMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLVAHYAKESSKH 336
           G+GYGPLA+GPRW+AYSGS I  S++       +  S   S S+S+ S VA +A++SSK 
Sbjct: 255 GVGYGPLAVGPRWIAYSGSRIATSSSA------IFTSEIVSLSTSSPS-VAQFARDSSKQ 314

Query: 337 LAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMADADSIGMAIVK 396
           LA+GI  LGDKG + L++Y SE+LP           GLKGIG  N  +ADA+SIGM IVK
Sbjct: 315 LASGIANLGDKGYRSLTKYCSEVLPNPY------IPGLKGIGVGNEKVADAESIGMVIVK 374

Query: 397 DIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAA 456
           DI +K+VITQFKAHKSPISALCFD SG +LVTAS+QGH+INVF+IMP+  +  ++  T  
Sbjct: 375 DITNKSVITQFKAHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTSRAVKTT-- 434

Query: 457 SYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPSAVIIAR 516
           +++HL+RLQRGFTNAVIQDI FS DSN I++ SSRGTSHLF INP              +
Sbjct: 435 TFAHLFRLQRGFTNAVIQDICFSKDSNLIVVGSSRGTSHLFEINP-------------EK 494

Query: 517 NGEPVVPARQTVRRVDSGLHMPKQNQCTTGSPLTLSAVTRIHHG-SNGWRGTVSSAAAAA 576
            G+                           +P+ +SA++RI  G S+GW GTVS AA+AA
Sbjct: 495 EGD---------------------------APVPMSAISRIRSGNSSGWIGTVSGAASAA 554

Query: 577 TGKM-GIVSGAIASAFHECKGNTVQVDNGSSE---VRYHILVFSPSGSMIQYALR----- 636
            G + G V G + S F  C   +     GS      + ++LVF+PSG M QYALR     
Sbjct: 555 AGMVAGSVPGTVTSTFCYCDEKSNNNYYGSVADMCSKTNLLVFAPSGCMTQYALREHQVG 614

Query: 637 VGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNRR-SQDNSIDIYGDDGGFN 696
           VG ++  +    S   E   E + +L V+ I++W++ Q Q+RR + D   DIYG     +
Sbjct: 615 VGHETAAMTGFDS---ESGLETEGKLAVDPIRRWSMIQNQSRRETHDPHSDIYGGGTSVD 674

Query: 697 CNKNYCEEMNGNPVLEAGGNIFKSKACR-EEQYHLYISEAELQMHAART--PLWTRPEIY 756
                  E+     +E    + K    R  ++ HLYI EAE Q     T  PLW R +  
Sbjct: 675 SKSKVFPEVVRKQSVEEAWKVSKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWARRKFR 698

Query: 757 FQVMARDGVKIDEIDHPG--ELDIERIPTRMIEARSKDLVPVFDYLQSSKISQ 794
           FQ +  +  + +EI   G  E++IE I TR IEAR++DLVPV+ YLQS +  Q
Sbjct: 735 FQELVLN--RGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRSQQ 698

BLAST of Chy4G077320 vs. TAIR 10
Match: AT1G03380.1 (homolog of yeast autophagy 18 (ATG18) G )

HSP 1 Score: 448.7 bits (1153), Expect = 1.1e-125
Identity = 303/781 (38.80%), Postives = 447/781 (57.23%), Query Frame = 0

Query: 43  GRAHEFLSTSFRAFSSYMKIVSAGASTVARS----AASVASSLVDKDDEANAFQVNWAGF 102
           G+    L  SF+  SS +K VSA A+ VA S     ASVA+S+   +D+ +  QV WAGF
Sbjct: 7   GKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAAEDDKD--QVTWAGF 66

Query: 103 DKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQVLPRLILSMRSG 162
             LE G +V R VLLLG ++GFQV+DVE+A+N  ELV +  G VS++Q+ P   L  RSG
Sbjct: 67  GILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQP---LPARSG 126

Query: 163 DK--FAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDGNFM--PTFVRFYS 222
           D   F  S PL+++ A G       +    + +G+ A   S +   G+ +  PT VRFYS
Sbjct: 127 DHEGFWNSHPLLLVVA-GDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINYPTTVRFYS 186

Query: 223 LKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKEHIILTNPVVSGFPGS 282
           L+S +YV+ L+FRS+V  ++CS  VVA+ LA QI+C+ A TLE +  +LT PV    P  
Sbjct: 187 LRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYPVPQ--PVR 246

Query: 283 GG----GMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNG--SLVAH 342
            G     +GYGP+A+GPRWLAY+    +   TGR+ PQ    S S S SSS+G  S +A 
Sbjct: 247 QGTTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGGSSFMAR 306

Query: 343 YAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMADAD 402
           YA ESSK LA G++ LGD G K LS+Y  ++LP+ + S  +    +  +G ++G  +DA+
Sbjct: 307 YAMESSKQLANGLINLGDMGYKTLSKYCQDMLPDGSTS-PASPNAIWKVGGVSG--SDAE 366

Query: 403 SIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSK 462
           + GM  VKD++S A+++QFKAH SPISALCFDPSGT+LVTASV G++INVF+IMPS    
Sbjct: 367 NAGMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNINVFQIMPSRSHN 426

Query: 463 SSISGT---AASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQ 522
           +    +    +S+ HL++L RG T+A++QDI FS  S W+ I SS+GT H+F +N +G  
Sbjct: 427 APGDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGTCHIFVLNSSGSD 486

Query: 523 VNFPSAVIIARNGEPV-VPARQTVRRVDSGLHMPKQNQCTTGSPLTLSAVTRIHHGSNGW 582
             F          EP  +PA          L    Q   +  + + LS V+RI + S GW
Sbjct: 487 AAFQP----CEGEEPTRLPASSLPWWFTQSL-SSNQQSLSPPTAVALSVVSRIKYSSFGW 546

Query: 583 RGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQVDNGSSEVRYHILVFSPSGSMIQYA 642
             TVS+A  AATGK+ + SGA+A+ FH+   + +Q+ N  +    HILV++PSG ++Q+ 
Sbjct: 547 LNTVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQL-NSRTNALEHILVYTPSGHVVQHE 606

Query: 643 LRVGLDSTIV---LPRSSTALELVSELDARLVVEAIQKWNISQKQN---------RRSQD 702
           L   + +      L    T+   V E D R+ VE IQ W++ ++ +         +   +
Sbjct: 607 LLPSVCTESPENGLRVQKTSHVQVQEDDLRVKVEPIQWWDVCRRSDWLETEERLPKSITE 666

Query: 703 NSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACRE---EQYHLYISEAELQMH 762
              D+         +++ C  ++ N        +   K+C E   E+ H Y+S  E+++ 
Sbjct: 667 KQYDLETVSNHLTSHEDACLSLDMNSHFSEDKYL---KSCSEKPPERSHCYLSNFEVKVT 726

Query: 763 AARTPLWTRPEIYFQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDYLQSS 791
           +   P+W   +I F VM  D  + D     GE +IE++P   +E + K L+PVFD+  S+
Sbjct: 727 SGMLPVWQNSKISFHVM--DSPR-DSSSTGGEFEIEKVPAHELEIKQKKLLPVFDHFHST 764

BLAST of Chy4G077320 vs. TAIR 10
Match: AT1G54710.1 (homolog of yeast autophagy 18 (ATG18) H )

HSP 1 Score: 416.4 bits (1069), Expect = 5.9e-116
Identity = 279/783 (35.63%), Postives = 430/783 (54.92%), Query Frame = 0

Query: 25  LGMRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDK 84
           + + N+G    Q    ++   + FL  S +  S+ ++  S+G   V  ++ASVA+SL   
Sbjct: 7   VNINNNGDNHNQ---TKNNGTNGFLPNSLKFISTCIRTASSG---VRSASASVAASL-SS 66

Query: 85  DDEANAFQVNWAGFDKLEWGDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSY 144
           D      QV W+ FD+L   ++  + VLLLG  +GFQV D++++N+V E V R D  V++
Sbjct: 67  DSHELKDQVLWSSFDRLHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTF 126

Query: 145 MQVLPRLILSMRSGDKFAESRPLVVLSAYGSIAANFNIQDQLASSGNAAIPKSQELVDG- 204
           +Q+ P L       + F  S P+++            + D+   SG     +   + +G 
Sbjct: 127 LQMQP-LPAKCDGVEGFRSSHPILLA-----------VADEAKGSGPIVTSRDGSVRNGY 186

Query: 205 ----NFMPTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCISATTLEKE 264
                  PT VRFYSL+S  YVH L+FRS VY V+CSP +VA+ L +QI+C  A TLE +
Sbjct: 187 EDPLALSPTVVRFYSLRSHNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENK 246

Query: 265 HIILTNPVVS-GFPG-SGGGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHL-KPSASF 324
             +L+ PV   G  G SG  +GYGP+A+G RWLAY+ +  L S+ GR+ PQ++  P  S 
Sbjct: 247 FSVLSYPVPQLGNQGISGVNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTPPGVSP 306

Query: 325 SHSSSNGSLVAHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKG 384
           S S +NG+LVA YA ESSKHLAAG++ LGDKG K +S+Y  +L  +      S + G K 
Sbjct: 307 STSPNNGNLVARYAMESSKHLAAGLLNLGDKGYKTISKYCQDLKHDGPGPSLSSSPGRK- 366

Query: 385 IGTLNGHMADADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSI 444
           +G +  H A++D +G  IVKD  S+A+I QF+AH SPISALCFDPSGT+LVTAS+ G++I
Sbjct: 367 VGRVGSHSAESDVVGTVIVKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHGNNI 426

Query: 445 NVFKIMPSSCSKSSISGT---AASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGT 504
           NVF+IMP+       + +   ++S+  LY+L RG T+AVIQDI FS  S WI I SS+ T
Sbjct: 427 NVFRIMPTPTKNGPGAQSYDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSSKST 486

Query: 505 SHLFAINPAGGQVNFPSAVIIARNGE---PVVPARQTVRRVDSGLHMPKQNQCTTGSPLT 564
            H++ ++P GG+      V+  RN +   P +    ++    S   M         + +T
Sbjct: 487 CHIYVLSPFGGE-----NVLEIRNSQFDGPTLAPTLSLPWWSSPSFMTTHFSYPPPASVT 546

Query: 565 LSAVTRIHHGSNGWRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVQVDNGSSEVRYH 624
           LS V+RI   +         AA++  GK    SG +A+ FH+   +  Q    SS    +
Sbjct: 547 LSVVSRIKCNN------FFHAASSVVGKPTFPSGCLAAVFHQ---SVPQESQSSSPALDY 606

Query: 625 ILVFSPSGSMIQYAL--RVGLDSTIVLPRSSTALELVSELDARLVVEAIQKWNISQKQNR 684
           +LV++PSG ++QY L   +G D      R+     L SE + R+ VE +Q W++ ++ + 
Sbjct: 607 LLVYTPSGHVVQYKLIPSLGGDQAESNTRNGATSGLTSEEELRVKVEPVQCWDVCRRADW 666

Query: 685 RSQDNSIDIYGDDGGFNCNKNYCEEMNGNPVLEAGGNIFKSKACREEQYHLYISEAELQM 744
             ++ +I          C   Y    N    ++   +  ++K    E++H+Y++ AE+ +
Sbjct: 667 PEREENI----------CGLTYGGRKNAELTVDTSDSEDQTKPL--EKHHVYLANAEVLI 726

Query: 745 HAARTPLWTRPEIYFQVM---ARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDY 789
           ++ R P+W   EI F  M     DG  ++     GE +I ++    ++ R KDL+PV+D 
Sbjct: 727 NSGRKPIWQNSEISFYPMYPPDSDGKNLNSHQGGGETEIGKVSANEVDIRRKDLLPVYDN 743

BLAST of Chy4G077320 vs. TAIR 10
Match: AT5G05150.1 (autophagy-related gene 18E )

HSP 1 Score: 52.0 bits (123), Expect = 2.9e-06
Identity = 31/99 (31.31%), Postives = 49/99 (49.49%), Query Frame = 0

Query: 403 VITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLY 462
           VI   KAH S I+ +     G++L TAS +G  I +F         +++ GT      L 
Sbjct: 196 VIKFIKAHDSAIACMTLTLDGSLLATASTKGTLIRIF---------NAVDGTL-----LQ 255

Query: 463 RLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINP 502
             +RG   A I +++ S +  W+  SS +GT H+F + P
Sbjct: 256 EFRRGVERAEIYNVAISSNLKWVAASSEKGTLHVFRLRP 280

BLAST of Chy4G077320 vs. TAIR 10
Match: AT2G40810.1 (homolog of yeast autophagy 18C )

HSP 1 Score: 47.4 bits (111), Expect = 7.2e-05
Identity = 27/91 (29.67%), Postives = 43/91 (47.25%), Query Frame = 0

Query: 409 AHKSPISALCFDPSGTILVTASVQGHSINVFKIMPSSCSKSSISGTAASYSHLYRLQRGF 468
           AH S I+ +     G +L TAS +G  I +F  M                + L  ++RG 
Sbjct: 199 AHDSSIACMTLTLDGLLLATASTKGTLIRIFNTMDG--------------TRLQEVRRGV 258

Query: 469 TNAVIQDISFSYDSNWIMISSSRGTSHLFAI 500
             A I  I+ S +  W+ +SS +GT H+F++
Sbjct: 259 DRADIYSIALSPNVQWLAVSSDKGTVHIFSL 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FH324.6e-13743.86Autophagy-related protein 18f OS=Arabidopsis thaliana OX=3702 GN=ATG18F PE=2 SV=... [more]
Q8GUL11.5e-12438.80Autophagy-related protein 18g OS=Arabidopsis thaliana OX=3702 GN=ATG18G PE=2 SV=... [more]
Q8H1Q58.4e-11535.63Autophagy-related protein 18h OS=Arabidopsis thaliana OX=3702 GN=ATG18H PE=2 SV=... [more]
Q8CCN54.2e-3427.68BCAS3 microtubule associated cell migration factor OS=Mus musculus OX=10090 GN=B... [more]
Q9H6U65.5e-3427.34BCAS3 microtubule associated cell migration factor OS=Homo sapiens OX=9606 GN=BC... [more]
Match NameE-valueIdentityDescription
A0A0A0LD330.0e+0097.79BCAS3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G415110 PE=4 ... [more]
A0A1S3BZN80.0e+0095.37LOW QUALITY PROTEIN: autophagy-related protein 18f OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5A7TTR50.0e+0095.35Autophagy-related protein 18f OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A6J1FA020.0e+0082.41autophagy-related protein 18f-like OS=Cucurbita moschata OX=3662 GN=LOC111443454... [more]
A0A6J1CE590.0e+0081.52autophagy-related protein 18f OS=Momordica charantia OX=3673 GN=LOC111010412 PE=... [more]
Match NameE-valueIdentityDescription
XP_004151961.20.097.68LOW QUALITY PROTEIN: autophagy-related protein 18f [Cucumis sativus][more]
XP_008454562.10.095.37PREDICTED: LOW QUALITY PROTEIN: autophagy-related protein 18f [Cucumis melo][more]
KAE8650685.10.094.16hypothetical protein Csa_011154 [Cucumis sativus][more]
KAA0046454.10.095.35autophagy-related protein 18f [Cucumis melo var. makuwa] >TYJ97759.1 autophagy-r... [more]
XP_038897831.10.089.00autophagy-related protein 18f [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G54730.13.2e-13843.86homolog of yeast autophagy 18 (ATG18) F [more]
AT1G03380.11.1e-12538.80homolog of yeast autophagy 18 (ATG18) G [more]
AT1G54710.15.9e-11635.63homolog of yeast autophagy 18 (ATG18) H [more]
AT5G05150.12.9e-0631.31autophagy-related gene 18E [more]
AT2G40810.17.2e-0529.67homolog of yeast autophagy 18C [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 400..440
e-value: 1.2E-4
score: 31.4
coord: 457..498
e-value: 84.0
score: 4.9
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 109..585
e-value: 2.4E-10
score: 42.0
IPR022175BCAS3 domainPFAMPF12490BCAS3coord: 546..781
e-value: 1.3E-66
score: 224.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 876..906
NoneNo IPR availablePANTHERPTHR13268:SF7AUTOPHAGY-RELATED PROTEIN 18Fcoord: 27..856
IPR045142BCAS3-likePANTHERPTHR13268BREAST CARCINOMA AMPLIFIED SEQUENCE 3coord: 27..856
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 113..500

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G077320.1Chy4G077320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006914 autophagy
biological_process GO:0042594 response to starvation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding