Chy4G074510 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G074510
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	E3 ubiquitin-protein ligase RNF144B-like
Location	chrH04: 9033523 .. 9035384 (+)
RNA-Seq Expression	Chy4G074510
Synteny	Chy4G074510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCTCCTTCTTCTTCTATCCTCCTCCAAAATCTCTCCGATGAAAATTACGCACAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCCTCTCCAATTAATCCATCCCCAACACCCCACTCTTCTACCTCTCAAGTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACGAACAGAGTATGTACACATACATTCTGCACCGCTTGCATCTCAAAGCACATCGCTGCAAAGCTTGAGGTTACAATGGCAGTGAAGTGTCCGGAGCCGAATTGTCGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGAATGCTCTGTTTGAAGCGATGATTCTGAAGTGGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATAATTGATGAAGGGGGTGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTTTGTGCAGAATGTAGAGTGGGATGGCATGGGGAAATGGAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGGCGGTGAAACTAGCTGAGAACAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTATGTTGAGAAGACTGTTGGTTGTGTTCATATCGTCTGCAGGTTTGTTCTCTAATTTACCCTTTACTTTTTGGTTTCTATGCTTCCAATTAATTCTTAACCTAGTTTGTGTTTAAATTGTAGAGCTATAAAATGAAGTTTTTTCGGAAAGAGGGATTAACCCACTGAATTAGTTAATAAGGCTGGTTAATAAAGTGGAGTTAGCCCAAGTGTTGATATGTGGATGGCCTTGTCATGATTCAAAGGGGTAGGTTGTATGGGTTTGGTATCGGGGTTGGGTTCGGACGAAAAACTAAGTTTGAGTTTGGATGTGGGTGTGGGTGTGGGTGTGGGGTTGACCTATGATCCAAAAGTAGTAAATGTAATATGAAAAATGAGGCGGTTTAAATATGATTGAACCAATTTTGAAGTTTAAATTTACAAAATAAAAGTAGCTAGAGGTTTGAGACGTTGATGTCAATTAAGGGGATTAAGCTTGTTTAGGTTTATGATAAGATTAATTTACTCATTTCCTTTTCCTTCTTATGCCTATGATAAGAGTAAATGAACATAAGAAACACTGTTGGATTCCTTAATTCTCTAAAATTGATGAAAATTTTAACTATAAACTTACTTTTATTGAAAATTGAAATAAAAATTATAAGATCTAATCTTTATGAAAAAAATTAAATGTTATAACAAAATAACAGTTCATGAATTCAATACTATTTAATATTTTTATTTGTATAACTTCTAATATTAAGGAAATTTTCAATAGAGTACGAAGTTCTCCTACTATTTCAGATGATTGTTTGACACTCCTTGAAAATTATGTGAAGTCAATCCAATAATTTTTTGATAGATTAATAAATTATTTAACAACACTGGAAAATTTTCACTTTAATAAAGGAGGACATGAACATAATGTTTATATGTTGAGTTATGTTAAAGTAGTGATCCCAAGGAAATGATAATGATAATATTAAGGGCAGTTAAATCAAATATTAATATAATAGAGAACAGCATACATAAAATAACTATGAAGATAAGTTGTATATGATGCAGTATTTATAATTAAGAATAAGATGCAATTGATGGAGATTTTAATTGAAGGATGAAATTTGTAGGTGTGGATCGGAATTTTGCTACAGCTGTGGAGCTAAGTGGGGTGGGGTTCATGCTTGTCAGGCTTCAACTTATGAACGTACTGTCTATCTACGTTGA

mRNA sequence

ATGGCCTCTCCTTCTTCTTCTATCCTCCTCCAAAATCTCTCCGATGAAAATTACGCACAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCCTCTCCAATTAATCCATCCCCAACACCCCACTCTTCTACCTCTCAAGTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACGAACAGAGTATGTACACATACATTCTGCACCGCTTGCATCTCAAAGCACATCGCTGCAAAGCTTGAGGTTACAATGGCAGTGAAGTGTCCGGAGCCGAATTGTCGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGAATGCTCTGTTTGAAGCGATGATTCTGAAGTGGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATAATTGATGAAGGGGGTGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTTTGTGCAGAATGTAGAGTGGGATGGCATGGGGAAATGGAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGGCGGTGAAACTAGCTGAGAACAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTATGTTGAGAAGACTGTTGGTTGTGTTCATATCGTCTGCAGGTGTGGATCGGAATTTTGCTACAGCTGTGGAGCTAAGTGGGGTGGGGTTCATGCTTGTCAGGCTTCAACTTATGAACGTACTGTCTATCTACGTTGA

Coding sequence (CDS)

ATGGCCTCTCCTTCTTCTTCTATCCTCCTCCAAAATCTCTCCGATGAAAATTACGCACAAGAGTTACAGCTTCAAGAAGTTATCACTTCCTCCTTATTATCCTCTCCAATTAATCCATCCCCAACACCCCACTCTTCTACCTCTCAAGTTCTCTGTTCAATCTGTACAGACGCAAAATCCCATTCCCAAATGTTCACGAACAGAGTATGTACACATACATTCTGCACCGCTTGCATCTCAAAGCACATCGCTGCAAAGCTTGAGGTTACAATGGCAGTGAAGTGTCCGGAGCCGAATTGTCGGACGGTGCTGGAGCCGGAGATGTGTGGATCCTTCGTACCGAAGAGGGTTTTGGAGCGGTGGGCGAATGCTCTGTTTGAAGCGATGATTCTGAAGTGGAAGAGATTGAATTGTCCGTTTAAGGATTGTGGGGCGGCGATAATTGATGAAGGGGGTGAGGAAGGGGTGACGGCGGTGGAATGTGGGAGTTGTTGGAGGTTGTTTTGTGCAGAATGTAGAGTGGGATGGCATGGGGAAATGGAATGTGGAGAGTTTCAGAGACTGAGGAAAGAGGCGGGTGTAAGTGGAGATAAAGATGATGCGATGGCGGTGAAACTAGCTGAGAACAAGAAATGGAGGAGATGTCCTCATTGTAAAATTTATGTTGAGAAGACTGTTGGTTGTGTTCATATCGTCTGCAGGTGTGGATCGGAATTTTGCTACAGCTGTGGAGCTAAGTGGGGTGGGGTTCATGCTTGTCAGGCTTCAACTTATGAACGTACTGTCTATCTACGTTGA

Protein sequence

MASPSSSILLQNLSDENYAQELQLQEVITSSLLSSPINPSPTPHSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCYSCGAKWGGVHACQASTYERTVYLR*

Homology

[Close]

BLAST of Chy4G074510 vs. ExPASy Swiss-Prot
Match: A4IIY1 (Probable E3 ubiquitin-protein ligase RNF144A OS=Xenopus tropicalis OX=8364 GN=rnf144a PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.8e-18
Identity = 55/201 (27.36%), Postives = 93/201 (46.27%), Query Frame = 0


Query: 50  VLCSICTDAKSHSQMFTNRVCTHTFCTACISKHIAAKLE--VTMAVKCPEPNC--RTVLE 109
           V C +C    +  QM T   C   FCT C+ +++   ++  +  A+ CP+ +C  R  L+
Sbjct: 18  VSCKLCLGEYTVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDASCPKRGHLQ 77

Query: 110 PEMCGSFVPKRVLERWANALFE-AMILKWKRLNCPFKDCGAAI-IDEGGEEGVTAVECGS 169
                  V   +++++    FE  ++L   R  CP   C A   + E G +    V+C +
Sbjct: 78  ENEIECMVAAEIMQKYKKLQFEKEILLDPCRTWCPSSSCQAVCKLQEKGIQNPQLVQCSA 137

Query: 170 CWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVE 229
           C   FC+ C+  WH    C E   +    G S     +    L ++   +RCP CK+Y+E
Sbjct: 138 CDIEFCSACKANWHPGQGCPENMAITFLPGDS----SSFFKSLEDDVPIKRCPKCKVYIE 197

Query: 230 KTVGCVHIVCR-CGSEFCYSC 244
           +  GC  ++C+ C   FC+ C
Sbjct: 198 RDEGCAQMMCKNCKHAFCWYC 214

[Close]

BLAST of Chy4G074510 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-16
Identity = 59/228 (25.88%), Postives = 97/228 (42.54%), Query Frame = 0


Query: 47  TSQVLCSICTDAKSHSQMFTNRVCTHTFCTACISKHIAAKLE---VTMAVKCPEPNCRTV 106
           +  V C IC +    +Q F   +C H +C  C   ++  K+      +   CP P C+ +
Sbjct: 134 SGNVSCLICLEDYPPTQTFA-LICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVI 193

Query: 107 LEPEMCGSFVPKRVLERWANALFEAMI-----LKWKRLNCPFKDCGAAI-IDEGGEEGVT 166
           +  +     V   V ER+ N + ++ +     +KW    CP   C  +I  D    +   
Sbjct: 194 VHQDAFKQIVSPEVFERFNNFILKSYVDDNPQVKW----CPAPGCIYSIRCDRKERKEAV 253

Query: 167 AVECGSCWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPH 226
             +CG  +   C +  +G H    C +  +  ++A    D+ + +   LA  KK   CP 
Sbjct: 254 NCKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKA---SDESENVTWMLANTKK---CPE 313

Query: 227 CKIYVEKTVGCVHIVCR-----CGSEFCYSCGAKW-------GGVHAC 254
           C+  +EK  GC+H+ CR     CG EFC+ C   W       GG + C
Sbjct: 314 CRSPIEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNC 350

[Close]

BLAST of Chy4G074510 vs. ExPASy Swiss-Prot
Match: P50876 (E3 ubiquitin-protein ligase RNF144A OS=Homo sapiens OX=9606 GN=RNF144A PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-16
Identity = 55/202 (27.23%), Postives = 92/202 (45.54%), Query Frame = 0


Query: 50  VLCSICTDAKSHSQMFTNRVCTHTFCTACISKHIAAKLE--VTMAVKCPEPNC--RTVLE 109
           V C +C       QM T   C   FCT C+ +++   ++  +  A+ CP+  C  +  L+
Sbjct: 18  VSCKLCLGEYPVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDAACPKQGHLQ 77

Query: 110 PEMCGSFVPKRVLERWANALFEAMIL-KWKRLNCPFKDCGAAI-IDEGGEEGVTAVECGS 169
                  V   +++R+    FE  +L    R  CP   C A   + + G +    V+C +
Sbjct: 78  ENEIECMVAAEIMQRYKKLQFEREVLFDPCRTWCPASTCQAVCQLQDVGLQTPQPVQCKA 137

Query: 170 CWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMAVKLAE-NKKWRRCPHCKIYV 229
           C   FC+ C+  WH    C E   +    G     + + A K+ E +   +RCP CK+Y+
Sbjct: 138 CRMEFCSTCKASWHPGQGCPETMPITFLPG-----ETSAAFKMEEDDAPIKRCPKCKVYI 197

Query: 230 EKTVGCVHIVCR-CGSEFCYSC 244
           E+  GC  ++C+ C   FC+ C
Sbjct: 198 ERDEGCAQMMCKNCKHAFCWYC 214

[Close]

BLAST of Chy4G074510 vs. ExPASy Swiss-Prot
Match: A5PK27 (E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.4e-16
Identity = 62/216 (28.70%), Postives = 96/216 (44.44%), Query Frame = 0


Query: 38  NPSPTPHSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTACISKH--IAAKLEVTMAVKC 97
           NP+P   +    V C +C   +S  +M T + C   FCTAC+ ++  +A +      + C
Sbjct: 17  NPTPGDLALVPLVTCKLCLCEQSLDKMTTLQECRCIFCTACLKQYMQLAIREGCGSPITC 76

Query: 98  PEPNC--RTVLEPEMCGSFVPKRVLERWANALFEAMI-LKWKRLNCPFKDCGAAIIDEGG 157
           P+  C     L+       VP    + +    FE  + L   R  CP  DC         
Sbjct: 77  PDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPCRTWCPVADCQTVCPVATS 136

Query: 158 EEG-VTAVECGSCWRLFCAECRVGWHGEMECGEFQR--LRKEAG-VSGDKDDAMAVKLAE 217
           + G    VEC SC   FC+ C+  WH E+ C + Q   L  E G + G + DA       
Sbjct: 137 DPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPGILPTEHGTLFGTETDAPI----- 196

Query: 218 NKKWRRCPHCKIYVEKTVGCVHIVCR-CGSEFCYSC 244
               ++CP C++Y+E+  GC  ++C+ C   FC+ C
Sbjct: 197 ----KQCPVCRVYIERNEGCAQMMCKNCKHTFCWYC 223

[Close]

BLAST of Chy4G074510 vs. ExPASy Swiss-Prot
Match: Q6NW85 (E3 ubiquitin-protein ligase arih1l OS=Danio rerio OX=7955 GN=arih1l PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.8e-16
Identity = 65/230 (28.26%), Postives = 96/230 (41.74%), Query Frame = 0


Query: 37  INPSPTP-------HSSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTACISKHIAAKL-- 96
           INPS  P        SS+  + C IC      +  FT   C H FC  C   ++  K+  
Sbjct: 140 INPSKKPKTRPMSTRSSSQDLPCQICY-LNYPNSYFTGLECGHKFCMQCWGDYLTTKIIE 199

Query: 97  -EVTMAVKCPEPNCRTVLEPE-----MCGSFVPKRVLERWANALFEA-MILKWKRLNCPF 156
             +   + CP  NC  +++       +  S V  +      N+  E   +LKW    CP 
Sbjct: 200 EGMGQTISCPAHNCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKW----CPA 259

Query: 157 KDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDD 216
            DC   +  +  +      +CG   R FC  C   WH  ++C   ++  K+     D D 
Sbjct: 260 PDCHHVVKVQYPDAKPVRCKCG---RQFCFNCGENWHDPVKCKWLRKWIKKC----DDDS 319

Query: 217 AMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCR---CGSEFCYSCGAKW 248
             +  +A N K   CP C + +EK  GC H+VCR   C +EFC+ C   W
Sbjct: 320 ETSNWIAANTK--ECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPW 355

[Close]

BLAST of Chy4G074510 vs. ExPASy TrEMBL
Match: A0A0A0L952 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.1e-147
Identity = 250/264 (94.70%), Postives = 256/264 (96.97%), Query Frame = 0


Query: 2   ASPSSSILLQNLSDENYAQELQLQEVITSSLLSSPINPSPTPHSSTSQVLCSICTDAKSH 61
           +S SSSILLQNLSDENYA+ELQLQEVITSSLLSSPINPSPTPHSSTSQ+LCSICTDAKSH
Sbjct: 6   SSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAKSH 65

Query: 62  SQMFTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERW 121
           SQMFTNRVCTHTFCTACIS HIAAKLEV MAVKCPEPNC TVLEPEMCGSFVPKRVLERW
Sbjct: 66  SQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERW 125

Query: 122 ANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEME 181
           A+ALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEME
Sbjct: 126 ADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEME 185

Query: 182 CGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCY 241
           CGEFQRLRKEAGVSGDKDDAM VKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGS+FCY
Sbjct: 186 CGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCY 245

Query: 242 SCGAKWGGVHACQASTYERTVYLR 266
           SCGAKWGG HAC+AS YERTVY R
Sbjct: 246 SCGAKWGGGHACKASAYERTVYRR 269

[Close]

BLAST of Chy4G074510 vs. ExPASy TrEMBL
Match: A0A5A7UED3 (E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00700 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 242/269 (89.96%), Postives = 251/269 (93.31%), Query Frame = 0


Query: 1   MASPSSSILLQNLSDENYAQELQLQEVITSSLLSS----PINPSPTPHSSTSQVLCSICT 60
           MASPSSSI   +LSDENYA+ELQLQEVITSSLLS     PI+PSPTPHSSTSQVLCSICT
Sbjct: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60

Query: 61  DAKSHSQMFTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKR 120
           DAKSHSQMFTNRVCTHTFCT CISKHIAAKLEVTMAVKCPEPNC TVLEPEMCGSFVPKR
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 121 VLERWANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGW 180
           VLERWA+ALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 181 HGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCG 240
           HGEM+CGEFQRLRKEAGVSGDKDDAM VKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCG
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240

Query: 241 SEFCYSCGAKWGGVHACQASTYERTVYLR 266
           SEFCYSCG KWGG HAC+ S YERTV+ R
Sbjct: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269

[Close]

BLAST of Chy4G074510 vs. ExPASy TrEMBL
Match: A0A1S3CDI0 (E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 242/269 (89.96%), Postives = 251/269 (93.31%), Query Frame = 0


Query: 1   MASPSSSILLQNLSDENYAQELQLQEVITSSLLSS----PINPSPTPHSSTSQVLCSICT 60
           MASPSSSI   +LSDENYA+ELQLQEVITSSLLS     PI+PSPTPHSSTSQVLCSICT
Sbjct: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60

Query: 61  DAKSHSQMFTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKR 120
           DAKSHSQMFTNRVCTHTFCT CISKHIAAKLEVTMAVKCPEPNC TVLEPEMCGSFVPKR
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 121 VLERWANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGW 180
           VLERWA+ALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 181 HGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCG 240
           HGEM+CGEFQRLRKEAGVSGDKDDAM VKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCG
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240

Query: 241 SEFCYSCGAKWGGVHACQASTYERTVYLR 266
           SEFCYSCG KWGG HAC+ S YERTV+ R
Sbjct: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269

[Close]

BLAST of Chy4G074510 vs. ExPASy TrEMBL
Match: A0A6J1GX07 (probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC111457924 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 5.0e-95
Identity = 167/256 (65.23%), Postives = 194/256 (75.78%), Query Frame = 0


Query: 13  LSDENYAQELQLQEVITSSLLSSPI---NPSPTPHSSTSQVLCSICTDAKSHSQMFTNRV 72
           +SDE +A+ELQLQE I SSL +S I    PSP P SS S  LC+ICTD KS S+MFT+  
Sbjct: 32  ISDEIFAEELQLQESIASSLFTSTIMSNKPSPNPESSGSTTLCTICTDTKSASEMFTSTG 91

Query: 73  CTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANALFEAM 132
           C+HTFC  CISKHIAAKLE +MAVKCPEP C  VLEPEMC SFVPK VLERW +AL +AM
Sbjct: 92  CSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKEVLERWGDALLKAM 151

Query: 133 ILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLR 192
           +LKWK + CPFKDC A ++D   EE + AVEC  CWR+FCA+C VGWHG+M C EFQRL 
Sbjct: 152 LLKWKMVYCPFKDCSAGMVD---EEDMEAVECPKCWRMFCAKCGVGWHGDMNCTEFQRLL 211

Query: 193 KEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCYSCGAKWGG 252
           K  GV  D+++ M +KLA+ +KW+RCPHCKI VEK  GC+HI CRCGSEFCY CGAKW G
Sbjct: 212 KNGGV--DREEEMTMKLADERKWKRCPHCKILVEKIEGCLHITCRCGSEFCYCCGAKWNG 271

Query: 253 VHACQASTYERTVYLR 266
            HAC  S YERT Y R
Sbjct: 272 HHACPPSAYERTAYRR 282

[Close]

BLAST of Chy4G074510 vs. ExPASy TrEMBL
Match: A0A6J1IU95 (probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC111479983 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 7.5e-91
Identity = 160/255 (62.75%), Postives = 192/255 (75.29%), Query Frame = 0


Query: 13  LSDENYAQELQLQEVITSSLLSSPIN--PSPTPHSSTSQVLCSICTDAKSHSQMFTNRVC 72
           +SDE +A+ELQLQE I SS  S+ ++  PSP P SS S+ LC+ICTD KS S+MFT+  C
Sbjct: 32  ISDEIFAEELQLQESIASSFTSTIMSNKPSPNPESSGSKTLCTICTDTKSASEMFTSTGC 91

Query: 73  THTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANALFEAMI 132
           +HTFC  CISKHIAAKL  ++AVKCPEP C  VL+PEMC SFVPK VLERW +AL +AM+
Sbjct: 92  SHTFCADCISKHIAAKLVDSLAVKCPEPKCAAVLKPEMCDSFVPKEVLERWGDALLKAML 151

Query: 133 LKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRK 192
           LKWK + CPFKDC A ++DE   E   AVEC  CWR+FCA+C VGWH +M C EFQRL K
Sbjct: 152 LKWKMVYCPFKDCSAGMVDEEDRE---AVECPKCWRMFCAKCGVGWHADMNCTEFQRLLK 211

Query: 193 EAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCYSCGAKWGGV 252
             GV  D+++ M +KLA+ +KW+RCPHCKI VEK +GC+HI CRCGSEFCYSCG KW   
Sbjct: 212 NGGV--DREEEMTMKLADERKWKRCPHCKILVEKILGCLHITCRCGSEFCYSCGGKWNDR 271

Query: 253 HACQASTYERTVYLR 266
           HAC  S YERTV  R
Sbjct: 272 HACPPSAYERTVNRR 281

[Close]

BLAST of Chy4G074510 vs. NCBI nr
Match: XP_004144476.2 (probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypothetical protein Csa_001774 [Cucumis sativus])

HSP 1 Score: 529 bits (1363), Expect = 1.95e-189
Identity = 250/264 (94.70%), Postives = 256/264 (96.97%), Query Frame = 0


Query: 2   ASPSSSILLQNLSDENYAQELQLQEVITSSLLSSPINPSPTPHSSTSQVLCSICTDAKSH 61
           +S SSSILLQNLSDENYA+ELQLQEVITSSLLSSPINPSPTPHSSTSQ+LCSICTDAKSH
Sbjct: 6   SSSSSSILLQNLSDENYAEELQLQEVITSSLLSSPINPSPTPHSSTSQLLCSICTDAKSH 65

Query: 62  SQMFTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERW 121
           SQMFTNRVCTHTFCTACIS HIAAKLEV MAVKCPEPNC TVLEPEMCGSFVPKRVLERW
Sbjct: 66  SQMFTNRVCTHTFCTACISNHIAAKLEVAMAVKCPEPNCGTVLEPEMCGSFVPKRVLERW 125

Query: 122 ANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEME 181
           A+ALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEME
Sbjct: 126 ADALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEME 185

Query: 182 CGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCY 241
           CGEFQRLRKEAGVSGDKDDAM VKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGS+FCY
Sbjct: 186 CGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSDFCY 245

Query: 242 SCGAKWGGVHACQASTYERTVYLR 265
           SCGAKWGG HAC+AS YERTVY R
Sbjct: 246 SCGAKWGGGHACKASAYERTVYRR 269

[Close]

BLAST of Chy4G074510 vs. NCBI nr
Match: XP_008460341.1 (PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa] >TYK19145.1 E3 ubiquitin-protein ligase RNF144B-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 506 bits (1303), Expect = 2.73e-180
Identity = 242/269 (89.96%), Postives = 251/269 (93.31%), Query Frame = 0


Query: 1   MASPSSSILLQNLSDENYAQELQLQEVITSSLLSSPI----NPSPTPHSSTSQVLCSICT 60
           MASPSSSI   +LSDENYA+ELQLQEVITSSLLS PI    +PSPTPHSSTSQVLCSICT
Sbjct: 1   MASPSSSIFQHHLSDENYAEELQLQEVITSSLLSPPIKIPIHPSPTPHSSTSQVLCSICT 60

Query: 61  DAKSHSQMFTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKR 120
           DAKSHSQMFTNRVCTHTFCT CISKHIAAKLEVTMAVKCPEPNC TVLEPEMCGSFVPKR
Sbjct: 61  DAKSHSQMFTNRVCTHTFCTDCISKHIAAKLEVTMAVKCPEPNCGTVLEPEMCGSFVPKR 120

Query: 121 VLERWANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGW 180
           VLERWA+ALFEAMILK KRLNCPFKDCGAA+IDEG EEGVTAVECGSCWRLFCAECRVGW
Sbjct: 121 VLERWADALFEAMILKSKRLNCPFKDCGAAMIDEGDEEGVTAVECGSCWRLFCAECRVGW 180

Query: 181 HGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCG 240
           HGEM+CGEFQRLRKEAGVSGDKDDAM VKLAENKKWRRCPHCKIYVEKTVGCVHI+CRCG
Sbjct: 181 HGEMKCGEFQRLRKEAGVSGDKDDAMTVKLAENKKWRRCPHCKIYVEKTVGCVHILCRCG 240

Query: 241 SEFCYSCGAKWGGVHACQASTYERTVYLR 265
           SEFCYSCG KWGG HAC+ S YERTV+ R
Sbjct: 241 SEFCYSCGGKWGGAHACRGSAYERTVHRR 269

[Close]

BLAST of Chy4G074510 vs. NCBI nr
Match: XP_038889081.1 (probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida])

HSP 1 Score: 377 bits (968), Expect = 3.66e-128
Identity = 177/263 (67.30%), Postives = 213/263 (80.99%), Query Frame = 0


Query: 5   SSSILLQNLSDENYAQELQLQEVITSSLLSSPI-----NPSPTPHSSTSQVLCSICTDAK 64
           SSS  L +LSDEN+A ELQLQE ITSSL S+P      N SP P  S    +C+ICTDAK
Sbjct: 13  SSSHFLHDLSDENFAYELQLQEAITSSLSSTPAEIPNNNSSPKPQDS----ICTICTDAK 72

Query: 65  SHSQMFTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLE 124
             S+MFTN +C+HTFCT CISK+IAAKLE +MAVKCP+P C T+LEPEMC SF+PK VLE
Sbjct: 73  LASEMFTNGLCSHTFCTDCISKYIAAKLEDSMAVKCPQPKCGTLLEPEMCRSFIPKEVLE 132

Query: 125 RWANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE 184
           RW +ALFEAMILKWKRLNCPFKDC A +I EG E+GVTAVEC +CWRLFCA+C+VGWHG+
Sbjct: 133 RWGDALFEAMILKWKRLNCPFKDCSAPMIYEGDEKGVTAVECLNCWRLFCAQCKVGWHGD 192

Query: 185 MECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEF 244
           MEC EF+++RKE     + +D M +KLA+ KKW++CP+CK+YVEKTVGCVHI+CRCG++F
Sbjct: 193 MECREFEKVRKE-----NTEDMMTMKLADEKKWKKCPNCKVYVEKTVGCVHIICRCGTQF 252

Query: 245 CYSCGAKWGGVHACQASTYERTV 262
           CYSCGAKWGG HAC  S YE ++
Sbjct: 253 CYSCGAKWGGGHACPNSAYEHSM 266

[Close]

BLAST of Chy4G074510 vs. NCBI nr
Match: XP_022956145.1 (probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata])

HSP 1 Score: 354 bits (908), Expect = 6.12e-120
Identity = 167/256 (65.23%), Postives = 194/256 (75.78%), Query Frame = 0


Query: 13  LSDENYAQELQLQEVITSSLLSSPI---NPSPTPHSSTSQVLCSICTDAKSHSQMFTNRV 72
           +SDE +A+ELQLQE I SSL +S I    PSP P SS S  LC+ICTD KS S+MFT+  
Sbjct: 32  ISDEIFAEELQLQESIASSLFTSTIMSNKPSPNPESSGSTTLCTICTDTKSASEMFTSTG 91

Query: 73  CTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANALFEAM 132
           C+HTFC  CISKHIAAKLE +MAVKCPEP C  VLEPEMC SFVPK VLERW +AL +AM
Sbjct: 92  CSHTFCADCISKHIAAKLEDSMAVKCPEPKCAAVLEPEMCDSFVPKEVLERWGDALLKAM 151

Query: 133 ILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLR 192
           +LKWK + CPFKDC A ++DE   E + AVEC  CWR+FCA+C VGWHG+M C EFQRL 
Sbjct: 152 LLKWKMVYCPFKDCSAGMVDE---EDMEAVECPKCWRMFCAKCGVGWHGDMNCTEFQRLL 211

Query: 193 KEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCYSCGAKWGG 252
           K  GV  D+++ M +KLA+ +KW+RCPHCKI VEK  GC+HI CRCGSEFCY CGAKW G
Sbjct: 212 KNGGV--DREEEMTMKLADERKWKRCPHCKILVEKIEGCLHITCRCGSEFCYCCGAKWNG 271

Query: 253 VHACQASTYERTVYLR 265
            HAC  S YERT Y R
Sbjct: 272 HHACPPSAYERTAYRR 282

[Close]

BLAST of Chy4G074510 vs. NCBI nr
Match: XP_022980686.1 (probable E3 ubiquitin-protein ligase ARI5 [Cucurbita maxima])

HSP 1 Score: 340 bits (871), Expect = 2.49e-114
Identity = 160/255 (62.75%), Postives = 192/255 (75.29%), Query Frame = 0


Query: 13  LSDENYAQELQLQEVITSSLLSSPIN--PSPTPHSSTSQVLCSICTDAKSHSQMFTNRVC 72
           +SDE +A+ELQLQE I SS  S+ ++  PSP P SS S+ LC+ICTD KS S+MFT+  C
Sbjct: 32  ISDEIFAEELQLQESIASSFTSTIMSNKPSPNPESSGSKTLCTICTDTKSASEMFTSTGC 91

Query: 73  THTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANALFEAMI 132
           +HTFC  CISKHIAAKL  ++AVKCPEP C  VL+PEMC SFVPK VLERW +AL +AM+
Sbjct: 92  SHTFCADCISKHIAAKLVDSLAVKCPEPKCAAVLKPEMCDSFVPKEVLERWGDALLKAML 151

Query: 133 LKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGEMECGEFQRLRK 192
           LKWK + CPFKDC A ++DE   E   AVEC  CWR+FCA+C VGWH +M C EFQRL K
Sbjct: 152 LKWKMVYCPFKDCSAGMVDEEDRE---AVECPKCWRMFCAKCGVGWHADMNCTEFQRLLK 211

Query: 193 EAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCYSCGAKWGGV 252
             GV  D+++ M +KLA+ +KW+RCPHCKI VEK +GC+HI CRCGSEFCYSCG KW   
Sbjct: 212 NGGV--DREEEMTMKLADERKWKRCPHCKILVEKILGCLHITCRCGSEFCYSCGGKWNDR 271

Query: 253 HACQASTYERTVYLR 265
           HAC  S YERTV  R
Sbjct: 272 HACPPSAYERTVNRR 281

[Close]

BLAST of Chy4G074510 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.2e-61
Identity = 112/266 (42.11%), Postives = 153/266 (57.52%), Query Frame = 0


Query: 13  LSDENYAQELQLQEVITSSLLSSP--INPSPTPHSS-------------------TSQVL 72
           ++DE+YA E+ LQE + SS  +S   +N  P  H +                    S+ L
Sbjct: 35  IADESYATEIHLQEAMFSSFGASTVGVNHHPQVHRNLVTLVKQEPEIKAENEPMEPSRRL 94

Query: 73  CSICTDAKSHSQMFTNRV-CTHTFCTACISKHIAAKL-EVTMAVKCPEPNCRTVLEPEMC 132
           C IC D K  S +F     CTH +CT C  +++A K+ E    +KCP+  C  ++EP  C
Sbjct: 95  CMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEPYTC 154

Query: 133 GSFVPKRVLERWANALFEAMILKWKRLNCPFKDCGAAII-DEGGEEGVTAVECGSCWRLF 192
              +PK V +RW   L E++I  W +  CPFKDC A ++ +E G+  VT  EC SC RLF
Sbjct: 155 RDLIPKDVFDRWEKILCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHRLF 214

Query: 193 CAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGC 252
           C +C+V WH  + C EFQR       S D+DDA+ +++A+NK+WRRCP CK YV+K  GC
Sbjct: 215 CVQCKVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKVEGC 274

Query: 253 VHIVCRCGSEFCYSCGAKWGGVHACQ 255
            HI CRCG +FCY CG+ W   HACQ
Sbjct: 275 QHIKCRCGYQFCYGCGSVWSSSHACQ 300

[Close]

BLAST of Chy4G074510 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 195.3 bits (495), Expect = 6.3e-50
Identity = 87/201 (43.28%), Postives = 124/201 (61.69%), Query Frame = 0


Query: 45  SSTSQVLCSICTDAKSHSQMFTNRVCTHTFCTACISKHIAAKLEVT-MAVKCPEPNCRTV 104
           S T+   C IC D+KS  + F    C+H +C  C+SK+IAAKL+   ++++CP   C   
Sbjct: 108 SKTATFDCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSGR 167

Query: 105 LEPEMCGSFVPKRVLERWANALFEAMILKWKRLNCPFKDCGAAIIDEGGEEGVTAVECGS 164
           LEP+ C   +PK V +RW +AL EA++++ K+  CP+KDC A +  E  E  +   EC  
Sbjct: 168 LEPDQCRQILPKEVFDRWGDALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPH 227

Query: 165 CWRLFCAECRVGWHGEMECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVE 224
           C R+ C EC   WH EM C EFQ+L   A     +DD +   +A+ KKW+RCP CK Y+E
Sbjct: 228 CHRMVCVECGTQWHPEMTCEEFQKL---AANERGRDDILLATMAKQKKWKRCPSCKFYIE 287

Query: 225 KTVGCVHIVCRCGSEFCYSCG 245
           K+ GC+++ CRCG  FCY+CG
Sbjct: 288 KSQGCLYMKCRCGLAFCYNCG 305

[Close]

BLAST of Chy4G074510 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-36
Identity = 78/257 (30.35%), Postives = 123/257 (47.86%), Query Frame = 0


Query: 5   SSSILLQNLSDENYAQELQLQEVITSSLLSSPINPSPTPHSSTSQVLCSICTDAKSHSQM 64
           S+  +L    D+N+A  L +  ++ +              +    + CSIC+D      M
Sbjct: 148 STGTVLVATRDDNFALRLAIDALVKA--------------TQEKPLTCSICSDKTDAEHM 207

Query: 65  FTNRVCTHTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANA 124
             N  C H  C +C+ + +  KL   +   C E  C++ L  E C   +  +++E W   
Sbjct: 208 LLNDKCLHRHCFSCVKQQVKVKLRSGIVPPCLEDGCKSELTLESCSMVLTPKLIEMWKRK 267

Query: 125 LFEAMILKWKRLNCPFKDCG-----AAIIDEGGEEGVTAVECGSCWRLFCAECRVGWHGE 184
           + E +I   +++ CP++ C        +  E  +  V A  C  C  LFC +C+V WH +
Sbjct: 268 MEEDLIPDAEKIYCPYRSCSMLMSKTELSREAEQSNVRA--CIKCSELFCIDCKVPWHSD 327

Query: 185 MECGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEF 244
           + C +++R+  E  V    +D M   LA ++ WR+C  CK  +E T GC HI CRCG EF
Sbjct: 328 LSCADYKRIHSERLV----NDMMLKVLANDQMWRQCSECKHMIELTEGCNHITCRCGYEF 384

Query: 245 CYSCGAKWGGVH--ACQ 255
           CY CG KW   H  +CQ
Sbjct: 388 CYRCGHKWTKYHHYSCQ 384

[Close]

BLAST of Chy4G074510 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 148.7 bits (374), Expect = 6.8e-36
Identity = 78/249 (31.33%), Postives = 119/249 (47.79%), Query Frame = 0


Query: 14  SDENYAQELQLQEVITSSLLSSPINPSPTPHSSTSQVL--CSICTDAKSHSQMFTNRVCT 73
           +D  +A +L  + ++      S + P   P  + + +   C+IC +      MF+   C 
Sbjct: 267 NDVKFAYKLARESIL------SMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCR 326

Query: 74  HTFCTACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANALFEAMIL 133
           H FC  C+ +H+  KL   MA KCP   C++ L  + CG  +  ++ + W   L E  I 
Sbjct: 327 HRFCFQCVKQHVEVKLLHGMAPKCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIP 386

Query: 134 KWKRLNCPFKDCGAAIIDEGGEEGVTAV----------ECGSCWRLFCAECRVGWHGEME 193
             +R+ CP+  C A +      E   ++           C  C  LFC +C+V WHG + 
Sbjct: 387 VTERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLS 446

Query: 194 CGEFQRLRKEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCY 251
           C E+++L  E       DD     LA NK WR+C  C+  +E + GC HI CRCG EFCY
Sbjct: 447 CTEYKKLHPEP----PADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCY 505

[Close]

BLAST of Chy4G074510 vs. TAIR 10
Match: AT3G45540.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 141.0 bits (354), Expect = 1.4e-33
Identity = 74/240 (30.83%), Postives = 123/240 (51.25%), Query Frame = 0


Query: 17  NYAQELQLQEVITSSLLSSPINPSPTPHSSTSQVLCSICTDAK-SHSQMFTNRVCTHTFC 76
           +YA +L  + ++  S +S  +NP P    +T +  C IC D   + +QMF    C H FC
Sbjct: 68  SYAYKLARETIV--SEISISVNP-PRQPKATRKTTCKICLDDDINENQMFCVGKCRHRFC 127

Query: 77  TACISKHIAAKLEVTMAVKCPEPNCRTVLEPEMCGSFVPKRVLERWANALFEAMILKWKR 136
           + C+ +HI  +L     ++CP   C+T L+   C + +  ++ E W   + E +I    R
Sbjct: 128 SDCMRRHIEVRLLEGSVMRCPHYRCKTTLKFGGCINLLTPKIREMWQQRIKEDLIPVTGR 187

Query: 137 LNCPFKDCGAAIIDEGGEEGVTAVE------CGSCWRLFCAECRVGWHGEMECGEFQRLR 196
           + CP   C A + +   E  ++  E      C  C ++FC +C+V WH  + C ++++L 
Sbjct: 188 IYCPNSRCSALMSET--ELSISTKEDEVRRCCFKCGQIFCIKCKVSWHSNLSCNDYKKLN 247

Query: 197 KEAGVSGDKDDAMAVKLAENKKWRRCPHCKIYVEKTVGCVHIVCRCGSEFCYSCGAKWGG 250
                   ++D     LA  K+WR+C  C+  +E + GCV + CRCG +FCY CG + GG
Sbjct: 248 PYP----TENDGKIKALANQKRWRQCGKCQHMIELSKGCVQVKCRCGHKFCYRCGVQAGG 298

The following BLAST results are available for this feature:

BLAST of Chy4G074510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4IIY1	2.8e-18	27.36	Probable E3 ubiquitin-protein ligase RNF144A OS=Xenopus tropicalis OX=8364 GN=rn...	[more]
Q6T486	1.2e-16	25.88	Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G...	[more]
P50876	1.5e-16	27.23	E3 ubiquitin-protein ligase RNF144A OS=Homo sapiens OX=9606 GN=RNF144A PE=1 SV=2	[more]
A5PK27	4.4e-16	28.70	E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1	[more]
Q6NW85	5.8e-16	28.26	E3 ubiquitin-protein ligase arih1l OS=Danio rerio OX=7955 GN=arih1l PE=2 SV=1	[more]

BLAST of Chy4G074510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L952	1.1e-147	94.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G646570 PE=3 SV=1	[more]
A0A5A7UED3	1.0e-140	89.96	E3 ubiquitin-protein ligase RNF144B-like protein OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3CDI0	1.0e-140	89.96	E3 ubiquitin-protein ligase RNF144B-like OS=Cucumis melo OX=3656 GN=LOC103499194...	[more]
A0A6J1GX07	5.0e-95	65.23	probable E3 ubiquitin-protein ligase ARI10 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1IU95	7.5e-91	62.75	probable E3 ubiquitin-protein ligase ARI5 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Chy4G074510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144476.2	1.95e-189	94.70	probable E3 ubiquitin-protein ligase RNF144A [Cucumis sativus] >KGN58470.1 hypot...	[more]
XP_008460341.1	2.73e-180	89.96	PREDICTED: E3 ubiquitin-protein ligase RNF144B-like [Cucumis melo] >KAA0053550.1...	[more]
XP_038889081.1	3.66e-128	67.30	probable E3 ubiquitin-protein ligase RNF144A-A [Benincasa hispida]	[more]
XP_022956145.1	6.12e-120	65.23	probable E3 ubiquitin-protein ligase ARI10 [Cucurbita moschata]	[more]
XP_022980686.1	2.49e-114	62.75	probable E3 ubiquitin-protein ligase ARI5 [Cucurbita maxima]	[more]

BLAST of Chy4G074510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14250.1	1.2e-61	42.11	RING/U-box superfamily protein	[more]
AT3G53690.1	6.3e-50	43.28	RING/U-box superfamily protein	[more]
AT5G37560.1	3.0e-36	30.35	RING/U-box superfamily protein	[more]
AT5G60250.1	6.8e-36	31.33	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G45540.1	1.4e-33	30.83	RING/U-box protein with C6HC-type zinc finger	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 197..253 e-value: 0.017 score: 21.5 coord: 119..182 e-value: 2.4E-13 score: 60.3
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 121..182 e-value: 5.1E-10 score: 39.4 coord: 205..251 e-value: 1.7E-8 score: 34.6
None	No IPR available	GENE3D	1.20.120.1750		coord: 184..263 e-value: 3.7E-15 score: 58.5
None	No IPR available	GENE3D	2.20.25.20		coord: 136..183 e-value: 3.7E-6 score: 29.0
None	No IPR available	PANTHER	PTHR11685:SF327	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 10..245
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 47..128
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 125..187
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 200..253
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 45..131 e-value: 8.7E-19 score: 69.2
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 10..245
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 70..79
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 48..264 score: 27.780682
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 52..96 score: 9.937009

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G074510.1	Chy4G074510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity