Chy4G071810 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G071810
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	RNA-directed DNA polymerase
Location	chrH04: 4294047 .. 4294322 (+)
RNA-Seq Expression	Chy4G071810
Synteny	Chy4G071810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCAAGTTTATCAAGAAAGTGTTGATCTGTCGTTATGGTCTCTCAGAGGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAAATTTGTGAGCAATTCAAGATCAATCACAGAAATTCGACTCCATACCGCCTAAAGATGAATCAGGCAGTTGAGGCAGCCAACAAAAATATTAAAAGAATCATTGAGAAGATGATAACAACATACAAAGATTGGCATGAAAATTTACCATTTGTGCTGCATGGATATCGCACATCA

mRNA sequence

ATGCTCAAGTTTATCAAGAAAGTGTTGATCTGTCGTTATGGTCTCTCAGAGGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAAATTTGTGAGCAATTCAAGATCAATCACAGAAATTCGACTCCATACCGCCTAAAGATGAATCAGGCAGTTGAGGCAGCCAACAAAAATATTAAAAGAATCATTGAGAAGATGATAACAACATACAAAGATTGGCATGAAAATTTACCATTTGTGCTGCATGGATATCGCACATCA

Coding sequence (CDS)

ATGCTCAAGTTTATCAAGAAAGTGTTGATCTGTCGTTATGGTCTCTCAGAGGGTATTATTACGGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAAATTTGTGAGCAATTCAAGATCAATCACAGAAATTCGACTCCATACCGCCTAAAGATGAATCAGGCAGTTGAGGCAGCCAACAAAAATATTAAAAGAATCATTGAGAAGATGATAACAACATACAAAGATTGGCATGAAAATTTACCATTTGTGCTGCATGGATATCGCACATCA

Protein sequence

MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAANKNIKRIIEKMITTYKDWHENLPFVLHGYRTS

Homology

[Close]

BLAST of Chy4G071810 vs. ExPASy TrEMBL
Match: A0A5A7UKG2 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G001050 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.0e-39
Identity = 82/92 (89.13%), Postives = 85/92 (92.39%), Query Frame = 0


Query: 1    MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
            MLKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR KMN+AVEAA
Sbjct: 1028 MLKFIKKELICRYGLLEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRSKMNEAVEAA 1087

Query: 61   NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 93
            NKNIKRIIEKM  TY+DWHE LPF LHGYRTS
Sbjct: 1088 NKNIKRIIEKMTITYQDWHEMLPFALHGYRTS 1119

[Close]

BLAST of Chy4G071810 vs. ExPASy TrEMBL
Match: A0A5D3BAL8 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00460 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.0e-39
Identity = 82/92 (89.13%), Postives = 85/92 (92.39%), Query Frame = 0


Query: 1    MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
            MLKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR KMN+AVEAA
Sbjct: 1112 MLKFIKKELICRYGLLEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRSKMNEAVEAA 1171

Query: 61   NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 93
            NKNIKRIIEKM  TY+DWHE LPF LHGYRTS
Sbjct: 1172 NKNIKRIIEKMTITYQDWHEMLPFALHGYRTS 1203

[Close]

BLAST of Chy4G071810 vs. ExPASy TrEMBL
Match: A0A5D3DAC8 (Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00320 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-39
Identity = 81/92 (88.04%), Postives = 85/92 (92.39%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR KMN+A+EAA
Sbjct: 42  VLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAA 101

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 93
           NKNIKRIIEKM  TYKDWHE LPF LHGYRTS
Sbjct: 102 NKNIKRIIEKMTITYKDWHEMLPFALHGYRTS 133

[Close]

BLAST of Chy4G071810 vs. ExPASy TrEMBL
Match: A0A5A7T570 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003710 PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.3e-39
Identity = 81/92 (88.04%), Postives = 85/92 (92.39%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR KMN+A+EAA
Sbjct: 42  VLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAA 101

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 93
           NKNIKRIIEKM  TYKDWHE LPF LHGYRTS
Sbjct: 102 NKNIKRIIEKMTITYKDWHEMLPFALHGYRTS 133

[Close]

BLAST of Chy4G071810 vs. ExPASy TrEMBL
Match: A0A5A7T0A1 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00070 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.0e-39
Identity = 82/92 (89.13%), Postives = 84/92 (91.30%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR KMN AVEAA
Sbjct: 645 VLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNGAVEAA 704

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 93
           NKNIKRIIEKM  TYKDWHE LPF LHGYRTS
Sbjct: 705 NKNIKRIIEKMTITYKDWHEMLPFALHGYRTS 736

[Close]

BLAST of Chy4G071810 vs. NCBI nr
Match: KAA0038143.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 171 bits (432), Expect = 5.20e-51
Identity = 81/92 (88.04%), Postives = 85/92 (92.39%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR KMN+A+EAA
Sbjct: 42  VLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAA 101

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 92
           NKNIKRIIEKM  TYKDWHE LPF LHGYRTS
Sbjct: 102 NKNIKRIIEKMTITYKDWHEMLPFALHGYRTS 133

[Close]

BLAST of Chy4G071810 vs. NCBI nr
Match: KAA0052135.1 (Pol polyprotein [Cucumis melo var. makuwa] >TYK05102.1 Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 167 bits (423), Expect = 1.01e-50
Identity = 80/92 (86.96%), Postives = 84/92 (91.30%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFIKK LICRYGL EGIITDNAKNLNNKM DE+CEQFKINH+NSTPYR KMN+ VEAA
Sbjct: 36  VLKFIKKELICRYGLPEGIITDNAKNLNNKMKDELCEQFKINHQNSTPYRPKMNETVEAA 95

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 92
           NKNIKRIIEKM  TYKDWHE LPFVLHGYRTS
Sbjct: 96  NKNIKRIIEKMTITYKDWHEMLPFVLHGYRTS 127

[Close]

BLAST of Chy4G071810 vs. NCBI nr
Match: TYK20464.1 (putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H [Cucumis melo var. makuwa])

HSP 1 Score: 171 bits (432), Expect = 3.82e-50
Identity = 81/92 (88.04%), Postives = 85/92 (92.39%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR KMN+A+EAA
Sbjct: 42  VLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRPKMNEAIEAA 101

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 92
           NKNIKRIIEKM  TYKDWHE LPF LHGYRTS
Sbjct: 102 NKNIKRIIEKMTITYKDWHEMLPFALHGYRTS 133

[Close]

BLAST of Chy4G071810 vs. NCBI nr
Match: KAA0036953.1 (Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 164 bits (414), Expect = 6.63e-49
Identity = 80/92 (86.96%), Postives = 82/92 (89.13%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFIKK LICRYGL EGIITDNAKNLNNKMMDE+CEQFKINHRNSTPYR  MN AVEAA
Sbjct: 42  VLKFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEQFKINHRNSTPYRPNMNGAVEAA 101

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 92
           NKNIKRIIEKM  TYKDWHE L F LHGYRTS
Sbjct: 102 NKNIKRIIEKMTITYKDWHEMLLFALHGYRTS 133

[Close]

BLAST of Chy4G071810 vs. NCBI nr
Match: KAA0043109.1 (Pol polyprotein [Cucumis melo var. makuwa] >TYJ96377.1 Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 160 bits (406), Expect = 3.65e-48
Identity = 77/92 (83.70%), Postives = 82/92 (89.13%), Query Frame = 0


Query: 1   MLKFIKKVLICRYGLSEGIITDNAKNLNNKMMDEICEQFKINHRNSTPYRLKMNQAVEAA 60
           +LKFI+K LICRYGL EGIITDNAKNLNNKM+DE+CEQFKINHRNST YR KMN  +EAA
Sbjct: 42  VLKFIRKELICRYGLLEGIITDNAKNLNNKMIDELCEQFKINHRNSTLYRPKMNGTIEAA 101

Query: 61  NKNIKRIIEKMITTYKDWHENLPFVLHGYRTS 92
           NKNIKRIIEKM  TYKDWHE LPF LHGYRTS
Sbjct: 102 NKNIKRIIEKMTITYKDWHEMLPFALHGYRTS 133

The following BLAST results are available for this feature:

BLAST of Chy4G071810 vs.


Match Name	E-value	Identity	Description

BLAST of Chy4G071810 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UKG2	1.0e-39	89.13	Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00105...	[more]
A0A5D3BAL8	1.0e-39	89.13	Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00460 ...	[more]
A0A5D3DAC8	1.3e-39	88.04	Putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H OS=...	[more]
A0A5A7T570	1.3e-39	88.04	Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G00371...	[more]
A0A5A7T0A1	3.0e-39	89.13	Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00070 ...	[more]

BLAST of Chy4G071810 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0038143.1	5.20e-51	88.04	Pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0052135.1	1.01e-50	86.96	Pol polyprotein [Cucumis melo var. makuwa] >TYK05102.1 Pol polyprotein [Cucumis ...	[more]
TYK20464.1	3.82e-50	88.04	putative RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H [Cu...	[more]
KAA0036953.1	6.63e-49	86.96	Pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0043109.1	3.65e-48	83.70	Pol polyprotein [Cucumis melo var. makuwa] >TYJ96377.1 Pol polyprotein [Cucumis ...	[more]

BLAST of Chy4G071810 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 50..70
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..90 e-value: 2.0E-21 score: 78.2
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 1..92 score: 10.863488
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 4..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G071810.1	Chy4G071810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0003676	nucleic acid binding