Chy4G071450 (gene) Cucumber (hystrix) v1

Overview
NameChy4G071450
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionSulfate adenylyltransferase
LocationchrH04: 3788692 .. 3790957 (+)
RNA-Seq ExpressionChy4G071450
SyntenyChy4G071450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGAGCCATTCACCTCGTTCCCTTCGCTCCTCGATCAAGTGCTCGTTGATCGACCCCGATGGTGGGGTTGTCGTCGACTTGGTTGTGCCTGAGAGTGAAAGGGCCTCGAGGGTTTTGGAGGCCGAGCCATTGCCGAAGGTTAAATTGACCCAAATTGATCTAGAATGGGTCCATGTCATTAGTGAAGGATGGGCGAGCCCGTTGAGAGGGTTCATGAGAGAAGATGAGTATTTGCAGAGTTTGCATTTCAATTGCATCAAAATTAAGGATGGGTCTTTTGTAAACATGTCGCTTCCTATTGTTCTTGCCATTGGCGACGATGTTAAGGACCAAATTGGGTCCTCATCTAATGTGGGGTTGCTTGGTCCCAATGGAGATTTGGTTGGTATTCTTCGCAGGTTAGTTTTTTTCTGAAGAAGGGTTGGTGAATCTAATTTCATTCGATCTTTTAGCCTCTATATATGGAGATGTGGAGATGGGCTTTTTTGGATTTTTATTTGAAGTATTGATTTATGAAATATGAGTTTGTTTTCTTTTCCACCCTATGTTTGGCCACCTATAATTGAAGTTATGATATGACACAAGTTAAATTATTTAAATCCTGAGAGATGCTCCGTTACAATTATAATAATTATAATATTCCCCTTGCTTGAAACAACTGTGTTGATCATGTCATATTTTATGTTGGAGAACAATATTTTTATAATGCTCCACAATCTCGTTTATAACATGTTTATTTGAAGTTCGAACAAAGGTGACAATGGTGATGATCTTAGATTTCTGGGTTTACAAATGCAACGCCTAATGTTGGATTTCTAGAAACTTTCTTTTTGTGAACAGTGAGGCCAATATGTACAGAAATATGTTTGGTCAAACTGAAACCAAATTATGTGATTTTCCATCCTCACTTTGAACAATTCTGCAGCATCGAGATATTCAAGCATAATAAGGAAGAGAGGATAGCTAGAACTTGGGGAACAACTGCACCTGGGTTACCATATGTTGATGAGGTCATTACAAATGCTGGGAACTGGCTTCTTGGTGGAGATCTTGAAGTAATAAACCCCATCAAATACAATGATGGACTTGATCATTATAGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCAATAGGCGTCAGGCCGATGCTGTTTTTGCCTTTCAATTGAGAAACCCTGTACATAATGGTCATGCTTTGTTGATGAATGATACACGAAAGCGACTTCTAGATATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAAGCCGATGATGTGCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTGTTTTCCACTATCATCATTCTAAAGAGTTATTTTCTTTATCATTCAAAAACTTCCACATTTTTATACTCTTTACTCTTTATAAGTGAAAATGTACAATTTTCTAACTTTTCCAACATTCAATAACTTGAAATGAAAGATTTGATTTTCTCGACCAAGGAAGTCAGTAGAATGTTAGTTGAATGGCATGATTCATCTCTACTCTTCAGTTAATATGAGTGCCGATTTTCTTGGCTGTTTACTTAGTTTCTGTGGTTTAAGAGAGACGTTCTATGTCTATAGGTTTTAGAAGATGGAATCCTTGACCCTGAAACGACCATCGTTTCCATTTTCCCATCACCAATGCATTACGCTGGTCCAACCGAAGTACAATGGCATGCTAAAGCAAGGATCAATGCAGGTGCAAATTTTTACATTGTTGGTCGAGATCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTAAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTCAGTATCTCAACGACCTTTCCTTCTCCTTTTCCTACTTTCCGCCCTCTTCATTTTAACATCCAGTTGACCGCAGGTAGCAGCATACAATACTGTTGCGAAGAAGATGGCATTCTTCGACCCTGCTCGTGCTAAAGATTTCCTCTTCATCTCTGGAACAAAGGTAAGAGCCAAGTTGAGTTTGGACAGGATGTCATTTTAATCCTGTTGTGACAATAGTAACTGAGTTGTACATGTGGTTTGTATTAGATGCGAACATACGCAAGGACAGGTGAGAATCCTCCTGATGGTTTCATGTGCCCTGGTGGATGGAAAGTGTTGGTCAATTATTACGAGAGCTTGCAGGCGGAAGAGGCGTCACCACAGCAGGCTGCATTGTCAGTTTAG

mRNA sequence

ATGCAGAGCCATTCACCTCGTTCCCTTCGCTCCTCGATCAAGTGCTCGTTGATCGACCCCGATGGTGGGGTTGTCGTCGACTTGGTTGTGCCTGAGAGTGAAAGGGCCTCGAGGGTTTTGGAGGCCGAGCCATTGCCGAAGGTTAAATTGACCCAAATTGATCTAGAATGGGTCCATGTCATTAGTGAAGGATGGGCGAGCCCGTTGAGAGGGTTCATGAGAGAAGATGAGTATTTGCAGAGTTTGCATTTCAATTGCATCAAAATTAAGGATGGGTCTTTTGTAAACATGTCGCTTCCTATTGTTCTTGCCATTGGCGACGATGTTAAGGACCAAATTGGGTCCTCATCTAATGTGGGGTTGCTTGGTCCCAATGGAGATTTGGTTGGTATTCTTCGCAGCATCGAGATATTCAAGCATAATAAGGAAGAGAGGATAGCTAGAACTTGGGGAACAACTGCACCTGGGTTACCATATGTTGATGAGGTCATTACAAATGCTGGGAACTGGCTTCTTGGTGGAGATCTTGAAGTAATAAACCCCATCAAATACAATGATGGACTTGATCATTATAGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCAATAGGCGTCAGGCCGATGCTGTTTTTGCCTTTCAATTGAGAAACCCTGTACATAATGGTCATGCTTTGTTGATGAATGATACACGAAAGCGACTTCTAGATATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAAGCCGATGATGTGCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTTAGAAGATGGAATCCTTGACCCTGAAACGACCATCGTTTCCATTTTCCCATCACCAATGCATTACGCTGGTCCAACCGAAGTACAATGGCATGCTAAAGCAAGGATCAATGCAGGTGCAAATTTTTACATTGTTGGTCGAGATCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTAAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTAGCAGCATACAATACTGTTGCGAAGAAGATGGCATTCTTCGACCCTGCTCGTGCTAAAGATTTCCTCTTCATCTCTGGAACAAAGATGCGAACATACGCAAGGACAGGTGAGAATCCTCCTGATGGTTTCATGTGCCCTGGTGGATGGAAAGTGTTGGTCAATTATTACGAGAGCTTGCAGGCGGAAGAGGCGTCACCACAGCAGGCTGCATTGTCAGTTTAG

Coding sequence (CDS)

ATGCAGAGCCATTCACCTCGTTCCCTTCGCTCCTCGATCAAGTGCTCGTTGATCGACCCCGATGGTGGGGTTGTCGTCGACTTGGTTGTGCCTGAGAGTGAAAGGGCCTCGAGGGTTTTGGAGGCCGAGCCATTGCCGAAGGTTAAATTGACCCAAATTGATCTAGAATGGGTCCATGTCATTAGTGAAGGATGGGCGAGCCCGTTGAGAGGGTTCATGAGAGAAGATGAGTATTTGCAGAGTTTGCATTTCAATTGCATCAAAATTAAGGATGGGTCTTTTGTAAACATGTCGCTTCCTATTGTTCTTGCCATTGGCGACGATGTTAAGGACCAAATTGGGTCCTCATCTAATGTGGGGTTGCTTGGTCCCAATGGAGATTTGGTTGGTATTCTTCGCAGCATCGAGATATTCAAGCATAATAAGGAAGAGAGGATAGCTAGAACTTGGGGAACAACTGCACCTGGGTTACCATATGTTGATGAGGTCATTACAAATGCTGGGAACTGGCTTCTTGGTGGAGATCTTGAAGTAATAAACCCCATCAAATACAATGATGGACTTGATCATTATAGGCTGTCTCCGAAACAACTTCGGCAAGAGTTCAATAGGCGTCAGGCCGATGCTGTTTTTGCCTTTCAATTGAGAAACCCTGTACATAATGGTCATGCTTTGTTGATGAATGATACACGAAAGCGACTTCTAGATATGGGTTACAAGAATCCAATTCTATTGCTTCATCCTTTGGGAGGTTTCACAAAAGCCGATGATGTGCCATTGGATGTTCGAATGGAGCAACATAGCAAGGTTTTAGAAGATGGAATCCTTGACCCTGAAACGACCATCGTTTCCATTTTCCCATCACCAATGCATTACGCTGGTCCAACCGAAGTACAATGGCATGCTAAAGCAAGGATCAATGCAGGTGCAAATTTTTACATTGTTGGTCGAGATCCTGCTGGAATGGGTCACCCAACAGAGAAGAGAGATTTATATGATCCAGACCATGGCAAGAAGGTCTTAAGTATGGCCCCTGGCCTTGAGAAGCTAAACATATTGCCCTTCAAGGTAGCAGCATACAATACTGTTGCGAAGAAGATGGCATTCTTCGACCCTGCTCGTGCTAAAGATTTCCTCTTCATCTCTGGAACAAAGATGCGAACATACGCAAGGACAGGTGAGAATCCTCCTGATGGTTTCATGTGCCCTGGTGGATGGAAAGTGTTGGTCAATTATTACGAGAGCTTGCAGGCGGAAGAGGCGTCACCACAGCAGGCTGCATTGTCAGTTTAG

Protein sequence

MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAALSV*
Homology
BLAST of Chy4G071450 vs. ExPASy Swiss-Prot
Match: Q43870 (ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 1.5e-215
Identity = 349/420 (83.10%), Postives = 388/420 (92.38%), Query Frame = 0

Query: 10  RSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPL 69
           + ++K SLIDPDGG +V+L+VPE+E   +  E+E +PKVKL QIDLEWVHVISEGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 70  RGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLV 129
           +GFMREDEYLQSLHFN +++K+G+FVNMSLPIVLAI DD K+QIGSS NV L+ P GD++
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 130 GILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLD 189
           G LRS+EI+KHNKEERIARTWGTT+PGLPYV+E IT +GNWL+GGDLEV  PIKYNDGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 190 HYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPL 249
           HYRLSPKQLR+EF+ RQADAVFAFQLRNPVHNGHALLMNDTRKRLL+MGYKNP+LLLHPL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 250 GGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARINAG 309
           GGFTKADDVPLDVRMEQHSKVLEDG+LDP+TTIVSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 310 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAKKMAF 369
           ANFYIVGRDPAGMGHPTEKRDLYDPDHGK+VLSMAPGLEKLNILPF+VAAY+T+ KKMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 370 FDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAALS 429
           FDP+RAK+FLFISGTKMRTYARTGENPPDGFMCP GW VLV YYESLQ  EA  QQA +S
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESLQESEAK-QQAVVS 475

BLAST of Chy4G071450 vs. ExPASy Swiss-Prot
Match: Q9LIK9 (ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.0e-191
Identity = 314/414 (75.85%), Postives = 356/414 (85.99%), Query Frame = 0

Query: 3   SHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVIS 62
           S S R    SI+  LI PDGG +V+L+V E +R  +  EA  LP+V+LT IDL+W+HV+S
Sbjct: 36  SKSLRRRVGSIRAGLIAPDGGKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLS 95

Query: 63  EGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLL 122
           EGWASPL GFMRE E+LQ+LHFN +++ DGS VNMS+PIVLAI D+ K +IG S+ V L 
Sbjct: 96  EGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALF 155

Query: 123 GPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPI 182
             +G+ V IL  IEI+KH KEERIARTWGTTAPGLPYVDE ITNAGNWL+GGDLEV+ P+
Sbjct: 156 NSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPV 215

Query: 183 KYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNP 242
           KYNDGLD +RLSP +LR+E  +R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP
Sbjct: 216 KYNDGLDRFRLSPAELRKELEKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNP 275

Query: 243 ILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHA 302
           ILLLHPLGGFTKADDVPLD RM+QH KVLEDG+LDPETT+VSIFPSPMHYAGPTEVQWHA
Sbjct: 276 ILLLHPLGGFTKADDVPLDWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHA 335

Query: 303 KARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNT 362
           KARINAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAY+ 
Sbjct: 336 KARINAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDK 395

Query: 363 VAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 417
              KMAFFDP+R +DFLFISGTKMRT A+  ENPPDGFMCPGGWKVLV+YYESL
Sbjct: 396 TQGKMAFFDPSRPQDFLFISGTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESL 449

BLAST of Chy4G071450 vs. ExPASy Swiss-Prot
Match: O23324 (ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 7.1e-189
Identity = 311/411 (75.67%), Postives = 354/411 (86.13%), Query Frame = 0

Query: 7   RSLRS-SIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 66
           RSLR+ S++  LI+PDGG +VDLVVPE  R  +  EA  LP+V+LT IDL+W+HV+SEGW
Sbjct: 41  RSLRTISVRAGLIEPDGGKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGW 100

Query: 67  ASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 126
           ASPLRGFMRE E+LQ+LHFN + + DGS VNMS+PIVLAI D  K  IG S  V L+  +
Sbjct: 101 ASPLRGFMRESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSD 160

Query: 127 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 186
            + + IL  IEI+KH KEERIARTWGTTAPGLPYV+E ITNAG+WL+GGDLEV+ P+KYN
Sbjct: 161 DNPIAILNDIEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYN 220

Query: 187 DGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 246
           DGLD +RLSP +LR+E  +R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNPILL
Sbjct: 221 DGLDRFRLSPFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILL 280

Query: 247 LHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKAR 306
           LHPLGGFTKADDVPL  RM+QH KVLEDG+LDPETT+VSIFPSPM YAGPTEVQWHAKAR
Sbjct: 281 LHPLGGFTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKAR 340

Query: 307 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAK 366
           INAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAY+    
Sbjct: 341 INAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQG 400

Query: 367 KMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 417
           KMAFFDP+RA+DFLFISGTKMR  A+  ENPPDGFMCPGGWKVLV+YY+SL
Sbjct: 401 KMAFFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSL 451

BLAST of Chy4G071450 vs. ExPASy Swiss-Prot
Match: Q9S7D8 (ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.3e-185
Identity = 307/409 (75.06%), Postives = 352/409 (86.06%), Query Frame = 0

Query: 12  SIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLP-KVKLTQIDLEWVHVISEGWASPLR 71
           S+K  LI+PDGG +++LVV ES R     EAE +P ++KL ++DLEWVHV+SEGWASPL+
Sbjct: 48  SVKSGLIEPDGGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLK 107

Query: 72  GFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVG 131
           GFMR+ E+LQ+LHFN  +++DGS VNMS+PIVLAI DD K +IG S+ V L+   G+ + 
Sbjct: 108 GFMRQSEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIA 167

Query: 132 ILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDH 191
           IL  IEI+KH KEERIARTWGTTA GLPY +E IT AGNWL+GGDL+V+ PIKYNDGLD 
Sbjct: 168 ILNDIEIYKHPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDR 227

Query: 192 YRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLG 251
           +RLSP QLR+EF RR ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLL+PLG
Sbjct: 228 FRLSPSQLREEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLG 287

Query: 252 GFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGA 311
           GFTKADDVPL  RM QH KVLEDG+LDPETT+VSIFPSPM YAGPTEVQWHAKARINAGA
Sbjct: 288 GFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGA 347

Query: 312 NFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAKKMAFF 371
           NFYIVGRDPAGMGHPTEKRDLYD DHGKKVLSMAPGLE+LNILPFKVAAY+    KMAFF
Sbjct: 348 NFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFF 407

Query: 372 DPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE 420
           DP+R++DFLFISGTKMR  A+  ENPPDGFMCP GWKVLV+YY+SL AE
Sbjct: 408 DPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAE 456

BLAST of Chy4G071450 vs. ExPASy Swiss-Prot
Match: O95340 (Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX=9606 GN=PAPSS2 PE=1 SV=2)

HSP 1 Score: 503.1 bits (1294), Expect = 3.2e-141
Identity = 235/391 (60.10%), Postives = 300/391 (76.73%), Query Frame = 0

Query: 27  DLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPLRGFMREDEYLQSLHFNC 86
           +L VPE++      EAE LP + +T++DL+WV V+SEGWA+PL+GFMRE EYLQ +HF+ 
Sbjct: 226 ELFVPENKLDHVRAEAETLPSLSITKLDLQWVQVLSEGWATPLKGFMREKEYLQVMHFD- 285

Query: 87  IKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVGILRSIEIFKHNKEERI 146
             + D   +NMS+PIVL +  + K ++   S   +L   G  V ILR  E ++H KEER 
Sbjct: 286 -TLLDDGVINMSIPIVLPVSAEDKTRLEGCSKF-VLAHGGRRVAILRDAEFYEHRKEERC 345

Query: 147 ARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDHYRLSPKQLRQEFNRRQ 206
           +R WGTT    P++ +++  +G+WL+GGDL+V+  I++NDGLD YRL+P +L+Q+     
Sbjct: 346 SRVWGTTCTKHPHI-KMVMESGDWLVGGDLQVLEKIRWNDGLDQYRLTPLELKQKCKEMN 405

Query: 207 ADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLGGFTKADDVPLDVRMEQ 266
           ADAVFAFQLRNPVHNGHALLM DTR+RLL+ GYK+P+LLLHPLGG+TK DDVPLD RM+Q
Sbjct: 406 ADAVFAFQLRNPVHNGHALLMQDTRRRLLERGYKHPVLLLHPLGGWTKDDDVPLDWRMKQ 465

Query: 267 HSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGANFYIVGRDPAGMGHPT 326
           H+ VLE+G+LDP++TIV+IFPSPM YAGPTEVQWH ++R+ AGANFYIVGRDPAGM HP 
Sbjct: 466 HAAVLEEGVLDPKSTIVAIFPSPMLYAGPTEVQWHCRSRMIAGANFYIVGRDPAGMPHPE 525

Query: 327 EKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAKKMAFFDPARAKDFLFISGTKM 386
            K+DLY+P HG KVLSMAPGL  + I+PF+VAAYN   K M F+DPAR  +F FISGT+M
Sbjct: 526 TKKDLYEPTHGGKVLSMAPGLTSVEIIPFRVAAYNKAKKAMDFYDPARHNEFDFISGTRM 585

Query: 387 RTYARTGENPPDGFMCPGGWKVLVNYYESLQ 418
           R  AR GENPPDGFM P  WKVL +YY SL+
Sbjct: 586 RKLAREGENPPDGFMAPKAWKVLTDYYRSLE 612

BLAST of Chy4G071450 vs. ExPASy TrEMBL
Match: A0A0A0LE71 (Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G872050 PE=4 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 2.5e-253
Identity = 427/430 (99.30%), Postives = 429/430 (99.77%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV
Sbjct: 54  MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 113

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG
Sbjct: 114 ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 173

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN
Sbjct: 174 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 233

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 234 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 293

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 294 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 353

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 354 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 413

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAKKMAFFDP RAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE
Sbjct: 414 DTVAKKMAFFDPTRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 473

Query: 421 ASPQQAALSV 431
           ASPQQAALSV
Sbjct: 474 ASPQQAALSV 483

BLAST of Chy4G071450 vs. ExPASy TrEMBL
Match: A0A5D3E7F0 (Sulfate adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G008170 PE=4 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.2e-249
Identity = 419/430 (97.44%), Postives = 425/430 (98.84%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ HSPRSLRSSIK SLIDPDGGV+VDLVVPESERASR+LEAEPLPKVKLTQIDLEWVHV
Sbjct: 1   MQGHSPRSLRSSIKSSLIDPDGGVIVDLVVPESERASRILEAEPLPKVKLTQIDLEWVHV 60

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGS VNMSLPIVLAIGDDVKDQIGSSSNVG
Sbjct: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSLVNMSLPIVLAIGDDVKDQIGSSSNVG 120

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVD VITNAGNWLLGGDLEVIN
Sbjct: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDVVITNAGNWLLGGDLEVIN 180

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 181 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 300

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAKKMAFFDP+RAKDFLFISGTKMR YARTGENPPDGFMCPGGWKVLVNYYESLQAEE
Sbjct: 361 DTVAKKMAFFDPSRAKDFLFISGTKMRAYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420

Query: 421 ASPQQAALSV 431
           ASPQQAALSV
Sbjct: 421 ASPQQAALSV 430

BLAST of Chy4G071450 vs. ExPASy TrEMBL
Match: A0A1S3CRF3 (Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103503887 PE=4 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.2e-249
Identity = 419/430 (97.44%), Postives = 425/430 (98.84%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ HSPRSLRSSIK SLIDPDGGV+VDLVVPESERASR+LEAEPLPKVKLTQIDLEWVHV
Sbjct: 54  MQGHSPRSLRSSIKSSLIDPDGGVIVDLVVPESERASRILEAEPLPKVKLTQIDLEWVHV 113

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGS VNMSLPIVLAIGDDVKDQIGSSSNVG
Sbjct: 114 ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSLVNMSLPIVLAIGDDVKDQIGSSSNVG 173

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVD VITNAGNWLLGGDLEVIN
Sbjct: 174 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDVVITNAGNWLLGGDLEVIN 233

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 234 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 293

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 294 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 353

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 354 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 413

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAKKMAFFDP+RAKDFLFISGTKMR YARTGENPPDGFMCPGGWKVLVNYYESLQAEE
Sbjct: 414 DTVAKKMAFFDPSRAKDFLFISGTKMRAYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 473

Query: 421 ASPQQAALSV 431
           ASPQQAALSV
Sbjct: 474 ASPQQAALSV 483

BLAST of Chy4G071450 vs. ExPASy TrEMBL
Match: A0A6J1FMY2 (Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446642 PE=4 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 6.8e-243
Identity = 404/430 (93.95%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ +SPRSLRSSIKCSLI+PDGG VV+L+VPESERASRVLEAE LPKVKLT+IDLEWVHV
Sbjct: 55  MQGYSPRSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHV 114

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPL+GFMRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAIGDDVK+QIGSS NVG
Sbjct: 115 ISEGWASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVG 174

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDE+ITNAGNWL+GGDLEVIN
Sbjct: 175 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEIITNAGNWLIGGDLEVIN 234

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 235 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 294

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 295 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 354

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 355 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 414

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAK+MAFFDP+RAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAE+
Sbjct: 415 DTVAKEMAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAED 474

Query: 421 ASPQQAALSV 431
           AS QQ ALSV
Sbjct: 475 ASSQQPALSV 484

BLAST of Chy4G071450 vs. ExPASy TrEMBL
Match: A0A6J1IXV9 (Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480215 PE=4 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 9.8e-242
Identity = 402/430 (93.49%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ +SPRSLRSSIKCSLI+PDGG VV+L+VPESERASRVLEAE LPKVKLT+IDLEWVHV
Sbjct: 55  MQGYSPRSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHV 114

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPL+GFMRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAIGDDVK+QIGSS NVG
Sbjct: 115 ISEGWASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVG 174

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWL+GGDLEVIN
Sbjct: 175 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVIN 234

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RR+ADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 235 PIKYNDGLDHYRLSPKQLRQEFDRREADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 294

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 295 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 354

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 355 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 414

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +T+AK+MAFF+P+RAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAE+
Sbjct: 415 DTMAKEMAFFNPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAED 474

Query: 421 ASPQQAALSV 431
           AS QQ ALSV
Sbjct: 475 ASSQQPALSV 484

BLAST of Chy4G071450 vs. NCBI nr
Match: XP_004136390.1 (ATP sulfurylase 2 [Cucumis sativus] >KGN60023.1 hypothetical protein Csa_001197 [Cucumis sativus])

HSP 1 Score: 881 bits (2277), Expect = 0.0
Identity = 427/430 (99.30%), Postives = 429/430 (99.77%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV
Sbjct: 54  MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 113

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG
Sbjct: 114 ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 173

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN
Sbjct: 174 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 233

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 234 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 293

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 294 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 353

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 354 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 413

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAKKMAFFDP RAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE
Sbjct: 414 DTVAKKMAFFDPTRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 473

Query: 421 ASPQQAALSV 430
           ASPQQAALSV
Sbjct: 474 ASPQQAALSV 483

BLAST of Chy4G071450 vs. NCBI nr
Match: XP_008466495.1 (PREDICTED: ATP sulfurylase 2 [Cucumis melo])

HSP 1 Score: 868 bits (2243), Expect = 0.0
Identity = 419/430 (97.44%), Postives = 425/430 (98.84%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ HSPRSLRSSIK SLIDPDGGV+VDLVVPESERASR+LEAEPLPKVKLTQIDLEWVHV
Sbjct: 54  MQGHSPRSLRSSIKSSLIDPDGGVIVDLVVPESERASRILEAEPLPKVKLTQIDLEWVHV 113

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGS VNMSLPIVLAIGDDVKDQIGSSSNVG
Sbjct: 114 ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSLVNMSLPIVLAIGDDVKDQIGSSSNVG 173

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVD VITNAGNWLLGGDLEVIN
Sbjct: 174 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDVVITNAGNWLLGGDLEVIN 233

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 234 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 293

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 294 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 353

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 354 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 413

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAKKMAFFDP+RAKDFLFISGTKMR YARTGENPPDGFMCPGGWKVLVNYYESLQAEE
Sbjct: 414 DTVAKKMAFFDPSRAKDFLFISGTKMRAYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 473

Query: 421 ASPQQAALSV 430
           ASPQQAALSV
Sbjct: 474 ASPQQAALSV 483

BLAST of Chy4G071450 vs. NCBI nr
Match: KAA0063274.1 (ATP sulfurylase 2 [Cucumis melo var. makuwa] >TYK31501.1 ATP sulfurylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 868 bits (2243), Expect = 0.0
Identity = 419/430 (97.44%), Postives = 425/430 (98.84%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ HSPRSLRSSIK SLIDPDGGV+VDLVVPESERASR+LEAEPLPKVKLTQIDLEWVHV
Sbjct: 1   MQGHSPRSLRSSIKSSLIDPDGGVIVDLVVPESERASRILEAEPLPKVKLTQIDLEWVHV 60

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGS VNMSLPIVLAIGDDVKDQIGSSSNVG
Sbjct: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSLVNMSLPIVLAIGDDVKDQIGSSSNVG 120

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVD VITNAGNWLLGGDLEVIN
Sbjct: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDVVITNAGNWLLGGDLEVIN 180

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 181 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 300

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAKKMAFFDP+RAKDFLFISGTKMR YARTGENPPDGFMCPGGWKVLVNYYESLQAEE
Sbjct: 361 DTVAKKMAFFDPSRAKDFLFISGTKMRAYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420

Query: 421 ASPQQAALSV 430
           ASPQQAALSV
Sbjct: 421 ASPQQAALSV 430

BLAST of Chy4G071450 vs. NCBI nr
Match: XP_038899984.1 (ATP sulfurylase 2 [Benincasa hispida])

HSP 1 Score: 852 bits (2202), Expect = 6.16e-311
Identity = 409/430 (95.12%), Postives = 421/430 (97.91%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ HSPRSLRSSIK SLIDPDGG +VDLVVPE+ERASRVLEAEPLPKVKLT+IDLEWVHV
Sbjct: 54  MQGHSPRSLRSSIKSSLIDPDGGALVDLVVPENERASRVLEAEPLPKVKLTRIDLEWVHV 113

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPLRGFMRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAI DDVKDQIGSSSNVG
Sbjct: 114 ISEGWASPLRGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIADDVKDQIGSSSNVG 173

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGP GDLVGILRSIEIFKHNKEER+ARTWGTT PGLPYVDEVITNAGNWL+GGDLEVIN
Sbjct: 174 LLGPTGDLVGILRSIEIFKHNKEERMARTWGTTVPGLPYVDEVITNAGNWLIGGDLEVIN 233

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 234 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 293

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 294 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 353

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHP EKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 354 HAKARINAGANFYIVGRDPAGMGHPKEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 413

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +TVAKKMAFFDP+RAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE+
Sbjct: 414 DTVAKKMAFFDPSRAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAED 473

Query: 421 ASPQQAALSV 430
           ASPQQ ALSV
Sbjct: 474 ASPQQPALSV 483

BLAST of Chy4G071450 vs. NCBI nr
Match: KAG6608587.1 (ATP sulfurylase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847 bits (2187), Expect = 1.23e-308
Identity = 404/430 (93.95%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 1   MQSHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHV 60
           MQ +SPRSLRSSIKCSLI+PDGG VV+L+VPESERASRVLEAE LPKVKLT+IDLEWVHV
Sbjct: 55  MQGYSPRSLRSSIKCSLIEPDGGAVVELIVPESERASRVLEAESLPKVKLTRIDLEWVHV 114

Query: 61  ISEGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVG 120
           ISEGWASPL+GFMRE+EYLQSLHFNC+KIKDGS VNMSLPIVLAIGDDVK+QIGSS NVG
Sbjct: 115 ISEGWASPLKGFMREEEYLQSLHFNCLKIKDGSLVNMSLPIVLAIGDDVKEQIGSSPNVG 174

Query: 121 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVIN 180
           LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWL+GGDLEVIN
Sbjct: 175 LLGPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLIGGDLEVIN 234

Query: 181 PIKYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 240
           PIKYNDGLDHYRLSPKQLRQEF+RRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK
Sbjct: 235 PIKYNDGLDHYRLSPKQLRQEFDRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYK 294

Query: 241 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQW 300
           NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDG+LDPETTIVSIFPSPMHYAGPTEVQW
Sbjct: 295 NPILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGVLDPETTIVSIFPSPMHYAGPTEVQW 354

Query: 301 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 360
           HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY
Sbjct: 355 HAKARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAY 414

Query: 361 NTVAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEE 420
           +T+AK+MAFFDP+RAKDFLFISGTKMRT+ARTGENPPDGFMCPGGWKVLVNYY+SLQAE+
Sbjct: 415 DTIAKEMAFFDPSRAKDFLFISGTKMRTFARTGENPPDGFMCPGGWKVLVNYYKSLQAED 474

Query: 421 ASPQQAALSV 430
           AS QQ ALSV
Sbjct: 475 ASSQQPALSV 484

BLAST of Chy4G071450 vs. TAIR 10
Match: AT1G19920.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 750.0 bits (1935), Expect = 1.1e-216
Identity = 349/420 (83.10%), Postives = 388/420 (92.38%), Query Frame = 0

Query: 10  RSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGWASPL 69
           + ++K SLIDPDGG +V+L+VPE+E   +  E+E +PKVKL QIDLEWVHVISEGWASPL
Sbjct: 57  KMTVKSSLIDPDGGELVELIVPETEIGVKKAESETMPKVKLNQIDLEWVHVISEGWASPL 116

Query: 70  RGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLV 129
           +GFMREDEYLQSLHFN +++K+G+FVNMSLPIVLAI DD K+QIGSS NV L+ P GD++
Sbjct: 117 KGFMREDEYLQSLHFNSLRLKNGTFVNMSLPIVLAIDDDTKEQIGSSENVALVCPQGDII 176

Query: 130 GILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLD 189
           G LRS+EI+KHNKEERIARTWGTT+PGLPYV+E IT +GNWL+GGDLEV  PIKYNDGLD
Sbjct: 177 GSLRSVEIYKHNKEERIARTWGTTSPGLPYVEEYITPSGNWLIGGDLEVFEPIKYNDGLD 236

Query: 190 HYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPL 249
           HYRLSPKQLR+EF+ RQADAVFAFQLRNPVHNGHALLMNDTRKRLL+MGYKNP+LLLHPL
Sbjct: 237 HYRLSPKQLREEFDNRQADAVFAFQLRNPVHNGHALLMNDTRKRLLEMGYKNPVLLLHPL 296

Query: 250 GGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARINAG 309
           GGFTKADDVPLDVRMEQHSKVLEDG+LDP+TTIVSIFPSPMHYAGPTEVQWHAKARINAG
Sbjct: 297 GGFTKADDVPLDVRMEQHSKVLEDGVLDPKTTIVSIFPSPMHYAGPTEVQWHAKARINAG 356

Query: 310 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAKKMAF 369
           ANFYIVGRDPAGMGHPTEKRDLYDPDHGK+VLSMAPGLEKLNILPF+VAAY+T+ KKMAF
Sbjct: 357 ANFYIVGRDPAGMGHPTEKRDLYDPDHGKRVLSMAPGLEKLNILPFRVAAYDTIEKKMAF 416

Query: 370 FDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAEEASPQQAALS 429
           FDP+RAK+FLFISGTKMRTYARTGENPPDGFMCP GW VLV YYESLQ  EA  QQA +S
Sbjct: 417 FDPSRAKEFLFISGTKMRTYARTGENPPDGFMCPSGWNVLVKYYESLQESEAK-QQAVVS 475

BLAST of Chy4G071450 vs. TAIR 10
Match: AT3G22890.1 (ATP sulfurylase 1 )

HSP 1 Score: 669.8 bits (1727), Expect = 1.4e-192
Identity = 314/414 (75.85%), Postives = 356/414 (85.99%), Query Frame = 0

Query: 3   SHSPRSLRSSIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVIS 62
           S S R    SI+  LI PDGG +V+L+V E +R  +  EA  LP+V+LT IDL+W+HV+S
Sbjct: 36  SKSLRRRVGSIRAGLIAPDGGKLVELIVEEPKRREKKHEAADLPRVELTAIDLQWMHVLS 95

Query: 63  EGWASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLL 122
           EGWASPL GFMRE E+LQ+LHFN +++ DGS VNMS+PIVLAI D+ K +IG S+ V L 
Sbjct: 96  EGWASPLGGFMRESEFLQTLHFNSLRLDDGSVVNMSVPIVLAIDDEQKARIGESTRVALF 155

Query: 123 GPNGDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPI 182
             +G+ V IL  IEI+KH KEERIARTWGTTAPGLPYVDE ITNAGNWL+GGDLEV+ P+
Sbjct: 156 NSDGNPVAILSDIEIYKHPKEERIARTWGTTAPGLPYVDEAITNAGNWLIGGDLEVLEPV 215

Query: 183 KYNDGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNP 242
           KYNDGLD +RLSP +LR+E  +R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP
Sbjct: 216 KYNDGLDRFRLSPAELRKELEKRNADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNP 275

Query: 243 ILLLHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHA 302
           ILLLHPLGGFTKADDVPLD RM+QH KVLEDG+LDPETT+VSIFPSPMHYAGPTEVQWHA
Sbjct: 276 ILLLHPLGGFTKADDVPLDWRMKQHEKVLEDGVLDPETTVVSIFPSPMHYAGPTEVQWHA 335

Query: 303 KARINAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNT 362
           KARINAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAY+ 
Sbjct: 336 KARINAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDK 395

Query: 363 VAKKMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 417
              KMAFFDP+R +DFLFISGTKMRT A+  ENPPDGFMCPGGWKVLV+YYESL
Sbjct: 396 TQGKMAFFDPSRPQDFLFISGTKMRTLAKNNENPPDGFMCPGGWKVLVDYYESL 449

BLAST of Chy4G071450 vs. TAIR 10
Match: AT4G14680.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 661.4 bits (1705), Expect = 5.0e-190
Identity = 311/411 (75.67%), Postives = 354/411 (86.13%), Query Frame = 0

Query: 7   RSLRS-SIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLPKVKLTQIDLEWVHVISEGW 66
           RSLR+ S++  LI+PDGG +VDLVVPE  R  +  EA  LP+V+LT IDL+W+HV+SEGW
Sbjct: 41  RSLRTISVRAGLIEPDGGKLVDLVVPEPRRREKKHEAADLPRVRLTAIDLQWMHVLSEGW 100

Query: 67  ASPLRGFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPN 126
           ASPLRGFMRE E+LQ+LHFN + + DGS VNMS+PIVLAI D  K  IG S  V L+  +
Sbjct: 101 ASPLRGFMRESEFLQTLHFNLLNLDDGSVVNMSVPIVLAIDDQQKALIGESKRVSLVDSD 160

Query: 127 GDLVGILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYN 186
            + + IL  IEI+KH KEERIARTWGTTAPGLPYV+E ITNAG+WL+GGDLEV+ P+KYN
Sbjct: 161 DNPIAILNDIEIYKHPKEERIARTWGTTAPGLPYVEEAITNAGDWLIGGDLEVLEPVKYN 220

Query: 187 DGLDHYRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILL 246
           DGLD +RLSP +LR+E  +R ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNPILL
Sbjct: 221 DGLDRFRLSPFELRKELEKRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPILL 280

Query: 247 LHPLGGFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKAR 306
           LHPLGGFTKADDVPL  RM+QH KVLEDG+LDPETT+VSIFPSPM YAGPTEVQWHAKAR
Sbjct: 281 LHPLGGFTKADDVPLSWRMKQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKAR 340

Query: 307 INAGANFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAK 366
           INAGANFYIVGRDPAGMGHP EKRDLYD DHGKKVLSMAPGLE+LNILPF+VAAY+    
Sbjct: 341 INAGANFYIVGRDPAGMGHPVEKRDLYDADHGKKVLSMAPGLERLNILPFRVAAYDKTQG 400

Query: 367 KMAFFDPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESL 417
           KMAFFDP+RA+DFLFISGTKMR  A+  ENPPDGFMCPGGWKVLV+YY+SL
Sbjct: 401 KMAFFDPSRAQDFLFISGTKMRALAKNRENPPDGFMCPGGWKVLVDYYDSL 451

BLAST of Chy4G071450 vs. TAIR 10
Match: AT5G43780.1 (Pseudouridine synthase/archaeosine transglycosylase-like family protein )

HSP 1 Score: 650.6 bits (1677), Expect = 8.9e-187
Identity = 307/409 (75.06%), Postives = 352/409 (86.06%), Query Frame = 0

Query: 12  SIKCSLIDPDGGVVVDLVVPESERASRVLEAEPLP-KVKLTQIDLEWVHVISEGWASPLR 71
           S+K  LI+PDGG +++LVV ES R     EAE +P ++KL ++DLEWVHV+SEGWASPL+
Sbjct: 48  SVKSGLIEPDGGKLMNLVVEESRRRVMKHEAETVPARIKLNRVDLEWVHVLSEGWASPLK 107

Query: 72  GFMREDEYLQSLHFNCIKIKDGSFVNMSLPIVLAIGDDVKDQIGSSSNVGLLGPNGDLVG 131
           GFMR+ E+LQ+LHFN  +++DGS VNMS+PIVLAI DD K +IG S+ V L+   G+ + 
Sbjct: 108 GFMRQSEFLQTLHFNSFRLEDGSVVNMSVPIVLAIDDDQKFRIGDSNQVTLVDSVGNPIA 167

Query: 132 ILRSIEIFKHNKEERIARTWGTTAPGLPYVDEVITNAGNWLLGGDLEVINPIKYNDGLDH 191
           IL  IEI+KH KEERIARTWGTTA GLPY +E IT AGNWL+GGDL+V+ PIKYNDGLD 
Sbjct: 168 ILNDIEIYKHPKEERIARTWGTTARGLPYAEEAITKAGNWLIGGDLQVLEPIKYNDGLDR 227

Query: 192 YRLSPKQLRQEFNRRQADAVFAFQLRNPVHNGHALLMNDTRKRLLDMGYKNPILLLHPLG 251
           +RLSP QLR+EF RR ADAVFAFQLRNPVHNGHALLM DTR+RLL+MGYKNP+LLL+PLG
Sbjct: 228 FRLSPSQLREEFIRRGADAVFAFQLRNPVHNGHALLMTDTRRRLLEMGYKNPVLLLNPLG 287

Query: 252 GFTKADDVPLDVRMEQHSKVLEDGILDPETTIVSIFPSPMHYAGPTEVQWHAKARINAGA 311
           GFTKADDVPL  RM QH KVLEDG+LDPETT+VSIFPSPM YAGPTEVQWHAKARINAGA
Sbjct: 288 GFTKADDVPLSWRMRQHEKVLEDGVLDPETTVVSIFPSPMLYAGPTEVQWHAKARINAGA 347

Query: 312 NFYIVGRDPAGMGHPTEKRDLYDPDHGKKVLSMAPGLEKLNILPFKVAAYNTVAKKMAFF 371
           NFYIVGRDPAGMGHPTEKRDLYD DHGKKVLSMAPGLE+LNILPFKVAAY+    KMAFF
Sbjct: 348 NFYIVGRDPAGMGHPTEKRDLYDADHGKKVLSMAPGLERLNILPFKVAAYDKTQGKMAFF 407

Query: 372 DPARAKDFLFISGTKMRTYARTGENPPDGFMCPGGWKVLVNYYESLQAE 420
           DP+R++DFLFISGTKMR  A+  ENPPDGFMCP GWKVLV+YY+SL AE
Sbjct: 408 DPSRSQDFLFISGTKMRGLAKKKENPPDGFMCPSGWKVLVDYYDSLSAE 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q438701.5e-21583.10ATP sulfurylase 2 OS=Arabidopsis thaliana OX=3702 GN=APS2 PE=1 SV=1[more]
Q9LIK92.0e-19175.85ATP sulfurylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS1 PE=1 SV... [more]
O233247.1e-18975.67ATP-sulfurylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS3 PE=1 SV... [more]
Q9S7D81.3e-18575.06ATP sulfurylase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APS4 PE=2 SV... [more]
O953403.2e-14160.10Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 OS=Homo sapiens OX... [more]
Match NameE-valueIdentityDescription
A0A0A0LE712.5e-25399.30Sulfate adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G872050 PE=4 SV=1[more]
A0A5D3E7F02.2e-24997.44Sulfate adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3CRF32.2e-24997.44Sulfate adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103503887 PE=4 SV=1[more]
A0A6J1FMY26.8e-24393.95Sulfate adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446642 PE=4 S... [more]
A0A6J1IXV99.8e-24293.49Sulfate adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480215 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
XP_004136390.10.099.30ATP sulfurylase 2 [Cucumis sativus] >KGN60023.1 hypothetical protein Csa_001197 ... [more]
XP_008466495.10.097.44PREDICTED: ATP sulfurylase 2 [Cucumis melo][more]
KAA0063274.10.097.44ATP sulfurylase 2 [Cucumis melo var. makuwa] >TYK31501.1 ATP sulfurylase 2 [Cucu... [more]
XP_038899984.16.16e-31195.12ATP sulfurylase 2 [Benincasa hispida][more]
KAG6608587.11.23e-30893.95ATP sulfurylase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT1G19920.11.1e-21683.10Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT3G22890.11.4e-19275.85ATP sulfurylase 1 [more]
AT4G14680.15.0e-19075.67Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
AT5G43780.18.9e-18775.06Pseudouridine synthase/archaeosine transglycosylase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 185..418
e-value: 1.1E-94
score: 318.1
NoneNo IPR availableGENE3D3.10.400.10Sulfate adenylyltransferasecoord: 16..184
e-value: 2.8E-56
score: 191.6
NoneNo IPR availablePANTHERPTHR11055BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASEcoord: 12..423
NoneNo IPR availablePANTHERPTHR11055:SF51ENDOGLUCANASEcoord: 12..423
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 190..413
IPR002650Sulphate adenylyltransferaseTIGRFAMTIGR00339TIGR00339coord: 20..410
e-value: 1.8E-126
score: 420.0
IPR002650Sulphate adenylyltransferaseCDDcd00517ATPScoord: 45..412
e-value: 2.10619E-166
score: 469.421
IPR025980ATP-sulfurylase PUA-like domainPFAMPF14306PUA_2coord: 18..180
e-value: 2.3E-43
score: 147.6
IPR024951Sulphate adenylyltransferase catalytic domainPFAMPF01747ATP-sulfurylasecoord: 190..411
e-value: 1.2E-66
score: 224.2
IPR015947PUA-like superfamilySUPERFAMILY88697PUA domain-likecoord: 20..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G071450.1Chy4G071450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009970 cellular response to sulfate starvation
biological_process GO:0016310 phosphorylation
biological_process GO:0000103 sulfate assimilation
cellular_component GO:0009507 chloroplast
molecular_function GO:0004020 adenylylsulfate kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004781 sulfate adenylyltransferase (ATP) activity