Chy4G071210 (gene) Cucumber (hystrix) v1

BLAST of Chy4G071210 vs. ExPASy Swiss-Prot
Match: F4I0P8 (Vacuolar protein sorting-associated protein 35B OS=Arabidopsis thaliana OX=3702 GN=VPS35B PE=1 SV=1)

HSP 1 Score: 1272.3 bits (3291), Expect = 0.0e+00
Identity = 638/798 (79.95%), Postives = 721/798 (90.35%), Query Frame = 0

Query: 44  GIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRYYELY 103
           G+EDE+KWLAEGIAGIQHNAF+MH+A+DAN+LRE LKYSA MLSELRTSKLSP +YY+LY
Sbjct: 6   GVEDEDKWLAEGIAGIQHNAFFMHRALDANNLREVLKYSALMLSELRTSKLSPQKYYDLY 65

Query: 104 MRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKEVPAK 163
           MRAFD+LR LEIFFKDESRHG+ VVDLYELVQHAGNILPR+YLLCTVGSVY+K+K+ P+K
Sbjct: 66  MRAFDQLRQLEIFFKDESRHGLPVVDLYELVQHAGNILPRMYLLCTVGSVYIKSKQAPSK 125

Query: 164 EVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVLQNFT 223
           +VLKDLVEMCRGVQHPIRGLFLR YLAQVSRD L +I S+ EGDA+TVM+AVEFVLQNFT
Sbjct: 126 DVLKDLVEMCRGVQHPIRGLFLRSYLAQVSRDKLPEIGSDYEGDANTVMDAVEFVLQNFT 185

Query: 224 EMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLPRVLE 283
           EMNKLWVRIQ QGP  +REKQEKER+ELRDLVGKNLHVL QIEGV+LEMYK+TVLPRVLE
Sbjct: 186 EMNKLWVRIQHQGPGTVREKQEKERNELRDLVGKNLHVLGQIEGVDLEMYKETVLPRVLE 245

Query: 284 QVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMDRLSN 343
           QVVNCKD+LAQYYLMECIIQVFPDEYHLQTLETLL  C QL  TVD ++VL+QLMDRLSN
Sbjct: 246 QVVNCKDKLAQYYLMECIIQVFPDEYHLQTLETLLAACTQLMPTVDTKIVLTQLMDRLSN 305

Query: 344 YAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLD 403
           YA S+ DV+ EFL VEAFAKLSNAIGKVI+ Q++MPIVGA+TL+VSLLTFTLRVHPDRLD
Sbjct: 306 YAASSPDVLHEFLQVEAFAKLSNAIGKVIDTQLEMPIVGAMTLFVSLLTFTLRVHPDRLD 365

Query: 404 YVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMDHLDN 463
           YVDQ+LGACV KLSS PKLEDARA KQ+V LLSAPLEKY+ IVTALTLSNYPRVMDHLD+
Sbjct: 366 YVDQVLGACVVKLSSVPKLEDARAMKQVVALLSAPLEKYSDIVTALTLSNYPRVMDHLDD 425

Query: 464 ATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKEEQNS 523
            TNKVMAM+IIQSIMK +SCISTADKV+VLFELIKGLIKDL+E + +ELDEEDF+EEQNS
Sbjct: 426 GTNKVMAMLIIQSIMKTDSCISTADKVEVLFELIKGLIKDLDETNAEELDEEDFQEEQNS 485

Query: 524 VARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQDGDV 583
           VARL+HML N++P EMLKIIC+V++H++ GGP RLPFTVPPL+FSA+ L+RQL++Q GD+
Sbjct: 486 VARLIHMLDNEEPEEMLKIICVVRRHLMTGGPRRLPFTVPPLVFSAVRLVRQLESQGGDI 545

Query: 584 VGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDCDLEH 643
            GE+            ATP+KIFQILNQ IE L+SVP PELALRLYL+CAEAA+DCDLE 
Sbjct: 546 AGEDS-----------ATPRKIFQILNQTIEVLTSVPCPELALRLYLQCAEAASDCDLEP 605

Query: 644 VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYSAKLL 703
           VAYEFFTQAF++YEE+IADSKAQVTAIHLI+GTLQR+NVFGVENRDTLTHKATGYSA+LL
Sbjct: 606 VAYEFFTQAFMLYEEEIADSKAQVTAIHLIVGTLQRINVFGVENRDTLTHKATGYSARLL 665

Query: 704 KKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSGPVTL 763
           KKPDQCRAVYACSHLFWVDDP+GIKDGERVLLCL+RALRIANAAQQMA+ TRGSSGPVTL
Sbjct: 666 KKPDQCRAVYACSHLFWVDDPDGIKDGERVLLCLRRALRIANAAQQMASATRGSSGPVTL 725

Query: 764 FVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTLRYIQ 823
           FVEILNKY+Y+FEKGN  IT + IQ LIELI  E+Q+D  + N     D FFTSTLRYI+
Sbjct: 726 FVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSD--NGNTTIHSDPFFTSTLRYIK 785

Query: 824 FQKQKGGVMGERYDSIKL 842
           F KQKGG+MGE+YD IKL
Sbjct: 786 FIKQKGGLMGEKYDPIKL 790

BLAST of Chy4G071210 vs. ExPASy Swiss-Prot
Match: Q7X659 (Vacuolar protein sorting-associated protein 35A OS=Arabidopsis thaliana OX=3702 GN=VPS35A PE=1 SV=1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 556/802 (69.33%), Postives = 679/802 (84.66%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           M++ G EDEEKWLA G A  + NAFYM +A+D+N+L++ LKYSAQMLSELRTSKLSPH+Y
Sbjct: 1   MIADGSEDEEKWLAAGAAAFKQNAFYMQRAIDSNNLKDALKYSAQMLSELRTSKLSPHKY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           Y+LYMRAFDELR LEIFF +E+R G +V++LYELVQHAGNILPRLYLLCT GSVY+K KE
Sbjct: 61  YDLYMRAFDELRKLEIFFMEETRRGCSVIELYELVQHAGNILPRLYLLCTAGSVYIKTKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
            PAKE+LKDLVEMCRG+QHP+RGLFLR YLAQ+SRD L DI SE EGDADTV++AVEFVL
Sbjct: 121 APAKEILKDLVEMCRGIQHPLRGLFLRSYLAQISRDKLPDIGSEYEGDADTVIDAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
            NFTEMNKLWVR+Q QGPAR +E++EKER ELRDLVGKNLHVLSQ+EGV+L+MY+ TVLP
Sbjct: 181 LNFTEMNKLWVRMQHQGPAREKERREKERGELRDLVGKNLHVLSQLEGVDLDMYRDTVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQ+VNC+DE+AQYYL++CIIQVFPDEYHLQTL+ LLG CPQLQA+VDI  VLS+LM+
Sbjct: 241 RVLEQIVNCRDEIAQYYLIDCIIQVFPDEYHLQTLDVLLGACPQLQASVDIMTVLSRLME 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYA  NA+V+P FL VEAF+KL+NAIGKVIEAQ DMPI+ A+TLY SLL FTL VHP
Sbjct: 301 RLSNYAALNAEVLPYFLQVEAFSKLNNAIGKVIEAQEDMPILSAVTLYSSLLKFTLHVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDY DQ+LG+CVK+LS + K++D RATK++V LLSAPLEKYN +VTAL L+NYP V++
Sbjct: 361 DRLDYADQVLGSCVKQLSGKGKIDDTRATKELVSLLSAPLEKYNDVVTALKLTNYPLVVE 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           +LD  T ++MA VI++SIMKNN+ I+TA+KV+ LFELIKG+I DL+E    E+DE+DF+E
Sbjct: 421 YLDTETKRIMATVIVRSIMKNNTLITTAEKVEALFELIKGIINDLDEPQGLEVDEDDFQE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVA L+HML+NDDP EM KI+ ++KKH L GGP RL FT+PPL+ S L LIR+L  +
Sbjct: 481 EQNSVALLIHMLYNDDPEEMFKIVNVLKKHFLTGGPKRLKFTIPPLVVSTLKLIRRLPVE 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
             +  G          +E   T  KIFQ LNQ+IEAL +VP+P+LA RLYL+CAEAA+ C
Sbjct: 541 GDNPFG----------KEASVTATKIFQFLNQIIEALPNVPSPDLAFRLYLQCAEAADKC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           D E +AYEFFTQA+I+YEE+I+DSKAQVTA+ LIIGTLQRM VFGVENRDTLTHKATGY+
Sbjct: 601 DEEPIAYEFFTQAYILYEEEISDSKAQVTALQLIIGTLQRMQVFGVENRDTLTHKATGYA 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFW++D E I+DGERVLLCLKRAL+IAN+AQQ+AN  RGS+G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWLEDRETIQDGERVLLCLKRALKIANSAQQVANTARGSTG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
            VTLF+EILNKYLY++EKG  QIT  +++ LI+LI  E   +S  ++P+   ++FF +TL
Sbjct: 721 SVTLFIEILNKYLYFYEKGVPQITVESVESLIKLIKNE---ESMPSDPSA--ESFFATTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 842
            +++FQKQK G +GERY +IK+
Sbjct: 781 EFMEFQKQKEGAIGERYQAIKV 787

BLAST of Chy4G071210 vs. ExPASy Swiss-Prot
Match: A8R7K9 (Vacuolar protein sorting-associated protein 35C OS=Arabidopsis thaliana OX=3702 GN=VPS35C PE=2 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 9.1e-305
Identity = 526/803 (65.50%), Postives = 656/803 (81.69%), Query Frame = 0

Query: 46  EDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRYYELYMR 105
           +D+EKWLA  IA ++ NAFYM +A+D+N+L++ LK+SAQMLSELRTSKLSPH+YYELYMR
Sbjct: 4   DDDEKWLAAAIAAVKQNAFYMQRAIDSNNLKDALKFSAQMLSELRTSKLSPHKYYELYMR 63

Query: 106 AFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKEVPAKEV 165
            F+EL  LEIFFK+E+  G ++ +LYELVQHAGNILPRLYLLCT+GSVY+K+K+V A ++
Sbjct: 64  VFNELGTLEIFFKEETGRGCSIAELYELVQHAGNILPRLYLLCTIGSVYIKSKDVTATDI 123

Query: 166 LKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVLQNFTEM 225
           LKDLVEMCR VQHP+RGLFLR YLAQV+RD L  I S+ EGD D  M A+EFVLQNFTEM
Sbjct: 124 LKDLVEMCRAVQHPLRGLFLRSYLAQVTRDKLPSIGSDLEGDGDAHMNALEFVLQNFTEM 183

Query: 226 NKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLPRVLEQV 285
           NKLWVR+Q QGP+R +EK+EKER+ELRDLVGKNLHVLSQ+EGV+L +Y+ TVLPR+LEQV
Sbjct: 184 NKLWVRMQHQGPSREKEKREKERNELRDLVGKNLHVLSQLEGVDLGIYRDTVLPRILEQV 243

Query: 286 VNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMDRLSNYA 345
           VNCKDELAQ YLM+CIIQVFPD++HLQTL+ LLG CPQLQ +VDI+ VLS LM+RLSNYA
Sbjct: 244 VNCKDELAQCYLMDCIIQVFPDDFHLQTLDVLLGACPQLQPSVDIKTVLSGLMERLSNYA 303

Query: 346 VSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLDYV 405
            S+ + +P FL VEAF+KL+ AIGKV+EAQ D+P   ++TLY+ LL FTL V+ DRLDYV
Sbjct: 304 ASSVEALPNFLQVEAFSKLNYAIGKVVEAQADLPAAASVTLYLFLLKFTLHVYSDRLDYV 363

Query: 406 DQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMDHLDNAT 465
           DQ+LG+CV +LS+  KL D +A KQIV  LSAPLEKYN +VT L L+NYP VM++LD  T
Sbjct: 364 DQVLGSCVTQLSATGKLCDDKAAKQIVAFLSAPLEKYNNVVTILKLTNYPLVMEYLDRET 423

Query: 466 NKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKEEQNSVA 525
           NK MA++++QS+ KNN+ I+TAD+VD LFEL KGL+KD +    DE+DEEDF+EEQN VA
Sbjct: 424 NKAMAIILVQSVFKNNTHIATADEVDALFELAKGLMKDFDGTIDDEIDEEDFQEEQNLVA 483

Query: 526 RLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQDGDVVG 585
           RL++ L+ DDP EM KII  V+KHI+ GGP RLP T+PPL+FSAL LIR+L+       G
Sbjct: 484 RLVNKLYIDDPEEMSKIIFTVRKHIVAGGPKRLPLTIPPLVFSALKLIRRLR-------G 543

Query: 586 EEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDCDLEHVA 645
            +++  GD+A    ATPK+I Q+L++ +E LS V AP+LALRLYL+CA+AAN+C+LE VA
Sbjct: 544 GDENPFGDDAS---ATPKRILQLLSETVEVLSDVSAPDLALRLYLQCAQAANNCELETVA 603

Query: 646 YEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYSAKLLKK 705
           YEFFT+A+++YEE+I+DSKAQVTA+ LIIGTLQRM VF VENRDTLTHKATGYSA+LL+K
Sbjct: 604 YEFFTKAYLLYEEEISDSKAQVTALRLIIGTLQRMRVFNVENRDTLTHKATGYSARLLRK 663

Query: 706 PDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRG--SSGPVTL 765
           PDQCRAVY C+HLFW D+ E +KDGERV+LCLKRA RIA+A QQMAN +RG  S+G V+L
Sbjct: 664 PDQCRAVYECAHLFWADECENLKDGERVVLCLKRAQRIADAVQQMANASRGTSSTGSVSL 723

Query: 766 FVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTLRYIQ 825
           +VE+LNKYLY+ EKGNQQ+T   I+ L ELI +E +     A P      F  STLRYI+
Sbjct: 724 YVELLNKYLYFLEKGNQQVTGDTIKSLAELIKSETKKVESGAEP------FINSTLRYIE 783

Query: 826 FQK-QKGGVMGERYDSIKLEGME 846
           FQ+ Q+ G M E+Y+ IK+E  E
Sbjct: 784 FQRQQEDGGMNEKYEKIKMEWFE 790

BLAST of Chy4G071210 vs. ExPASy Swiss-Prot
Match: Q2HJG5 (Vacuolar protein sorting-associated protein 35 OS=Bos taurus OX=9913 GN=VPS35 PE=2 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 2.4e-172
Identity = 352/812 (43.35%), Postives = 509/812 (62.68%), Query Frame = 0

Query: 46  EDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRYYELYMR 105
           +++EK L E I  ++  +F M + +D N L + LK+++ ML ELRTS LSP  YYELYM 
Sbjct: 10  DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMA 69

Query: 106 AFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKEVPAKEV 165
             DEL  LE++  DE   G  V DLYELVQ+AGNI+PRLYLL TVG VY+K+     K++
Sbjct: 70  ISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDI 129

Query: 166 LKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGE-GDADT---VMEAVEFVLQN 225
           LKDLVEMCRGVQHP+RGLFLR+YL Q +R+IL D   EGE  D +T   + ++++FVL N
Sbjct: 130 LKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPD---EGEPTDEETTGDISDSMDFVLLN 189

Query: 226 FTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLPRV 285
           F EMNKLWVR+Q QG +R REK+E+ER ELR LVG NL  LSQ+EGVN+E YKQ VL  +
Sbjct: 190 FAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGI 249

Query: 286 LEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMDRL 345
           LEQVVNC+D LAQ YLMECIIQVFPDE+HLQTL   L  C +L   V+++ ++  L+DRL
Sbjct: 250 LEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRL 309

Query: 346 SNYA-VSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHPD 405
           + +A   +   IP    ++ F   S  +  VI+++ DMP    ++L VSL+   ++ +PD
Sbjct: 310 ALFAHREDGPGIP--TDIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPD 369

Query: 406 RLDYVDQILGACVK---KLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRV 465
           R+DYVD++L   V+   KL+ +     +  +K++  LL  P++ YN I+T L L ++  +
Sbjct: 370 RVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPL 429

Query: 466 MDHLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDF 525
            ++ D  + K M+  ++ +++  N+ I + D+VD +  L+  LI+D  +  V+E D EDF
Sbjct: 430 FEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEEPDPEDF 489

Query: 526 KEEQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQ 585
            +EQ+ V R +H+L ++DP +   I+   +KH   GG  R+ FT+PPL+F+A  L  +  
Sbjct: 490 ADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRY- 549

Query: 586 NQDGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAAN 645
                   +E  +V D+ E+     +KIF   +Q I AL      EL LRL+L+ A AA 
Sbjct: 550 --------KENSKVDDKWEK---KCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAG 609

Query: 646 DCDLEH---VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHK 705
           +   E+   VAYEF +QAF +YE++I+DSKAQ+ AI LIIGT +RM  F  EN + L  +
Sbjct: 610 EIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQ 669

Query: 706 ATGYSAKLLKKPDQCRAVYACSHLFWV-----DDPEGIKDGERVLLCLKRALRIANAAQQ 765
               ++KLLKKPDQ RAV  C+HLFW       + E +  G+RV+ CLK+AL+IAN    
Sbjct: 670 CALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMD 729

Query: 766 MANVTRGSSGPVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAT 825
                   S  V LF+EILN+Y+Y++EK N  +T   +  LI+ I  +L  +  S+    
Sbjct: 730 -------PSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDL-PNLESSEETE 789

Query: 826 APDAFFTSTLRYIQFQKQKGGVMGERYDSIKL 842
             +  F +TL +++ +++     G  Y+ + L
Sbjct: 790 QINKHFHNTLEHLRLRRESPESEGPIYEGLIL 796

BLAST of Chy4G071210 vs. ExPASy Swiss-Prot
Match: Q9EQH3 (Vacuolar protein sorting-associated protein 35 OS=Mus musculus OX=10090 GN=Vps35 PE=1 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 4.1e-172
Identity = 351/812 (43.23%), Postives = 509/812 (62.68%), Query Frame = 0

Query: 46  EDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRYYELYMR 105
           +++EK L E I  ++  +F M + +D N L + LK+++ ML ELRTS LSP  YYELYM 
Sbjct: 10  DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMA 69

Query: 106 AFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKEVPAKEV 165
             DEL  LE++  DE   G  V DLYELVQ+AGNI+PRLYLL TVG VY+K+     K++
Sbjct: 70  ISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDI 129

Query: 166 LKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGE-GDADT---VMEAVEFVLQN 225
           LKDLVEMCRGVQHP+RGLFLR+YL Q +R+IL D   EGE  D +T   + ++++FVL N
Sbjct: 130 LKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPD---EGEPTDEETTGDISDSMDFVLLN 189

Query: 226 FTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLPRV 285
           F EMNKLWVR+Q QG +R REK+E+ER ELR LVG NL  LSQ+EGVN+E YKQ VL  +
Sbjct: 190 FAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGI 249

Query: 286 LEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMDRL 345
           LEQVVNC+D LAQ YLMECIIQVFPDE+HLQTL   L  C +L   V+++ ++  L+DRL
Sbjct: 250 LEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRL 309

Query: 346 SNYA-VSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHPD 405
           + +A   +   IP    ++ F   S  +  VI+++ DMP    ++L VSL+   ++ +PD
Sbjct: 310 ALFAHREDGPGIP--AEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPD 369

Query: 406 RLDYVDQILGACVK---KLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRV 465
           R+DYVD++L   V+   KL+ +     +  +K++  LL  P++ YN I+T L L ++  +
Sbjct: 370 RVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPL 429

Query: 466 MDHLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDF 525
            ++ D  + K M+  ++ +++  N+ I + D+VD +  L+  LI+D  +  V++ D EDF
Sbjct: 430 FEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDF 489

Query: 526 KEEQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQ 585
            +EQ+ V R +H+L +DDP +   I+   +KH   GG  R+ FT+PPL+F+A  L  +  
Sbjct: 490 ADEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRY- 549

Query: 586 NQDGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAAN 645
                   +E  ++ D+ E+     +KIF   +Q I AL      EL LRL+L+ A AA 
Sbjct: 550 --------KENSQMDDKWEK---KCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAG 609

Query: 646 DCDLEH---VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHK 705
           +   E+   VAYEF +QAF +YE++I+DSKAQ+ AI LIIGT +RM  F  EN + L  +
Sbjct: 610 EIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQ 669

Query: 706 ATGYSAKLLKKPDQCRAVYACSHLFWV-----DDPEGIKDGERVLLCLKRALRIANAAQQ 765
               ++KLLKKPDQ RAV  C+HLFW       + E +  G+RV+ CLK+AL+IAN    
Sbjct: 670 CALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMD 729

Query: 766 MANVTRGSSGPVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAT 825
                   S  V LF+EILN+Y+Y++EK N  +T   +  LI+ I  +L  +  S+    
Sbjct: 730 -------PSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDL-PNLESSEETE 789

Query: 826 APDAFFTSTLRYIQFQKQKGGVMGERYDSIKL 842
             +  F +TL +++ +++     G  Y+ + L
Sbjct: 790 QINKHFHNTLEHLRSRRESPESEGPIYEGLIL 796

BLAST of Chy4G071210 vs. ExPASy TrEMBL
Match: A0A1S3CSM2 (Vacuolar protein sorting-associated protein 35 OS=Cucumis melo OX=3656 GN=LOC103503864 PE=3 SV=1)

HSP 1 Score: 1555.4 bits (4026), Expect = 0.0e+00
Identity = 794/802 (99.00%), Postives = 799/802 (99.63%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPARLREKQEKER+ELRDLVGKNLHVLSQIEGV+LEMYKQTVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERNELRDLVGKNLHVLSQIEGVDLEMYKQTVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEE+SVDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEEVSVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDPGEMLKIIC VKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPGEMLKIICAVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPA APDAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAPAPDAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 842
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 802

BLAST of Chy4G071210 vs. ExPASy TrEMBL
Match: A0A0A0LE86 (Vacuolar protein sorting-associated protein 35 OS=Cucumis sativus OX=3659 GN=Csa_3G873280 PE=3 SV=1)

HSP 1 Score: 1555.0 bits (4025), Expect = 0.0e+00
Identity = 794/802 (99.00%), Postives = 797/802 (99.38%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVKKLSSQPK+EDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKKLSSQPKIEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEE SVDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEEASVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDPGEMLKIIC VKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPGEMLKIICTVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPA APDAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAPAPDAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 842
           RYIQFQKQKGGVMGERYDSI +
Sbjct: 781 RYIQFQKQKGGVMGERYDSINV 802

BLAST of Chy4G071210 vs. ExPASy TrEMBL
Match: A0A6J1BQM1 (Vacuolar protein sorting-associated protein 35 OS=Momordica charantia OX=3673 GN=LOC111004898 PE=3 SV=1)

HSP 1 Score: 1489.9 bits (3856), Expect = 0.0e+00
Identity = 764/802 (95.26%), Postives = 781/802 (97.38%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLSVGIEDEEKWLAEGIAGIQHNAFYMHQA+DAN+LRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLR+YLAQVSRDILLDINSE EGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGV+LEMYKQ VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVDLEMYKQAVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYA SNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVK LSSQPKLED RATKQIVMLLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKNLSSQPKLEDTRATKQIVMLLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIMKNNSCISTADKV+VLFELIKGLIKDLEE++ DELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVEVLFELIKGLIKDLEEVTADELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDP EMLKIIC VKKHILCGGPNRLPFTVPPLI SAL+LIR+LQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPVEMLKIICTVKKHILCGGPNRLPFTVPPLILSALSLIRRLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVV EEQ E GD  EEMHATPKKIFQ+LNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVREEQGESGDVEEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEE+IADSKAQVT+IHLIIGTLQRMNVFGVENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEEIADSKAQVTSIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFWVD+PEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDEPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPS  PA+  DAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSTTPAS--DAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 842
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 800

BLAST of Chy4G071210 vs. ExPASy TrEMBL
Match: A0A6J1J4Z2 (Vacuolar protein sorting-associated protein 35 OS=Cucurbita maxima OX=3661 GN=LOC111481301 PE=3 SV=1)

HSP 1 Score: 1472.6 bits (3811), Expect = 0.0e+00
Identity = 751/802 (93.64%), Postives = 778/802 (97.01%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLS+GIEDEEKWLAEGIAGIQHNAFYMHQA+DAN+LRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGV VVDLYELVQHAGN+LPRLYLLCTVGSVYMK KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLR+YLAQVSRDILLDINSE EGDADTVMEAVEF+L
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFML 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPA LREKQEKER+ELRDLVGKNLHVLSQIEGV+LEMYKQ+VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYA SNADVIP+FLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVK LSSQPKLED RATKQIV+LLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIM +NSCISTADKVDVLFELIKGLIKDLEE++VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDP EMLKII  VKKHILCGGP+RLPFTVPPLIFSAL+LIR+LQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIIYAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVVGEEQ E GD  EEMHATPKKIFQ+LNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQ+MNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQC+AVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGS G
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           P+TLFVEILNKYL+YFEKGNQQITNAAIQGLIELI TEL TD+PSANP +  DAFFTSTL
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPS--DAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 842
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 800

BLAST of Chy4G071210 vs. ExPASy TrEMBL
Match: A0A6J1FTJ5 (Vacuolar protein sorting-associated protein 35 OS=Cucurbita moschata OX=3662 GN=LOC111446704 PE=3 SV=1)

HSP 1 Score: 1471.4 bits (3808), Expect = 0.0e+00
Identity = 749/802 (93.39%), Postives = 778/802 (97.01%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLS+GIEDEEKWLAEGIAGIQHNAFYMHQA+DAN+LRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGV VVDLYELVQHAGN+LPRLYLLC+VGSVYMK K+
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCSVGSVYMKTKD 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLR+YLAQVSRDILLDINSE EGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPA LREKQEKER+ELRDLVGKNLHVLSQIEGV+LEMYKQ+VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYA SNADVIP+FLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVK LSSQPKLED RATKQIV+LLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIM +NSCISTADKVDVLFELIKGLIKDLEE++VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDP EMLKIIC VKKHILCGGP+RLPFTVPPLIFSAL+LIR+LQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           D DVVGEEQ E GD  EEMHATPKKIFQ+LNQLIEALSSVPAPELALRLY+ECAEAANDC
Sbjct: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYMECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQ+MNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQC+AVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGS G
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           P+TLFVEILNKYL+YFEKGNQQITNAAIQGLIELI TEL TD+PSANP +  DAFFTSTL
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPS--DAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 842
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 800

BLAST of Chy4G071210 vs. NCBI nr
Match: XP_008466470.1 (PREDICTED: vacuolar protein sorting-associated protein 35B isoform X1 [Cucumis melo])

HSP 1 Score: 1550 bits (4014), Expect = 0.0
Identity = 794/802 (99.00%), Postives = 799/802 (99.63%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPARLREKQEKER+ELRDLVGKNLHVLSQIEGV+LEMYKQTVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERNELRDLVGKNLHVLSQIEGVDLEMYKQTVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEE+SVDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEEVSVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDPGEMLKIIC VKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPGEMLKIICAVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPA APDAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAPAPDAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 841
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 802

BLAST of Chy4G071210 vs. NCBI nr
Match: XP_011652456.1 (vacuolar protein sorting-associated protein 35B isoform X1 [Cucumis sativus] >KGN60048.1 hypothetical protein Csa_001380 [Cucumis sativus])

HSP 1 Score: 1550 bits (4013), Expect = 0.0
Identity = 794/802 (99.00%), Postives = 797/802 (99.38%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVKKLSSQPK+EDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKKLSSQPKIEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEE SVDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEEASVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDPGEMLKIIC VKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPGEMLKIICTVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPA APDAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAPAPDAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 841
           RYIQFQKQKGGVMGERYDSI +
Sbjct: 781 RYIQFQKQKGGVMGERYDSINV 802

BLAST of Chy4G071210 vs. NCBI nr
Match: XP_038898001.1 (vacuolar protein sorting-associated protein 35B-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1512 bits (3914), Expect = 0.0
Identity = 774/802 (96.51%), Postives = 790/802 (98.50%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLSV IEDEEKWLAEGIAGIQHNAFYMHQA+DANSLRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVAIEDEEKWLAEGIAGIQHNAFYMHQAVDANSLREALKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMK+K+
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKD 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHP+RGLFLR+YLAQVSRDILLDINSE EGDAD VMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPLRGLFLRNYLAQVSRDILLDINSECEGDADAVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGV+LEMYKQTVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVDLEMYKQTVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYAVSNADVIP+FLHVEAFAKLSNAIGKVIEAQ DMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAVSNADVIPDFLHVEAFAKLSNAIGKVIEAQADMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVKKLSSQPKLED RATKQIVMLLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKKLSSQPKLEDTRATKQIVMLLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEE++VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEEVTVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLH+LHNDDPGEMLKIIC VKKHILCGGPNRLPFTVPPLIFSALNLIR+LQNQ
Sbjct: 481 EQNSVARLLHVLHNDDPGEMLKIICAVKKHILCGGPNRLPFTVPPLIFSALNLIRRLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVVGEEQHEVGDE EEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQHEVGDEVEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGIENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSA+PA  PD+FFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSASPA--PDSFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 841
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 800

BLAST of Chy4G071210 vs. NCBI nr
Match: XP_022131851.1 (vacuolar protein sorting-associated protein 35B-like [Momordica charantia])

HSP 1 Score: 1485 bits (3844), Expect = 0.0
Identity = 764/802 (95.26%), Postives = 781/802 (97.38%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLSVGIEDEEKWLAEGIAGIQHNAFYMHQA+DAN+LRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLR+YLAQVSRDILLDINSE EGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGV+LEMYKQ VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVDLEMYKQAVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYA SNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVK LSSQPKLED RATKQIVMLLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKNLSSQPKLEDTRATKQIVMLLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIMKNNSCISTADKV+VLFELIKGLIKDLEE++ DELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVEVLFELIKGLIKDLEEVTADELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDP EMLKIIC VKKHILCGGPNRLPFTVPPLI SAL+LIR+LQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPVEMLKIICTVKKHILCGGPNRLPFTVPPLILSALSLIRRLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           DGDVV EEQ E GD  EEMHATPKKIFQ+LNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVREEQGESGDVEEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEE+IADSKAQVT+IHLIIGTLQRMNVFGVENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEEIADSKAQVTSIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFWVD+PEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDEPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPS  PA+  DAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSTTPAS--DAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 841
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 800

BLAST of Chy4G071210 vs. NCBI nr
Match: KAG6608605.1 (Vacuolar protein sorting-associated protein 35B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1469 bits (3804), Expect = 0.0
Identity = 751/802 (93.64%), Postives = 778/802 (97.01%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           MLS+GIEDEEKWLAEGIAGIQHNAFYMHQA+DAN+LRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           YELYMRAFDELRMLEIFFKDESRHGV VVDLYELVQHAGN+LPRLYLLCTVGSVYMK KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
           VPAKEVLKDLVEMCRGVQHPIRGLFLR+YLAQVSRDILLDINSE EGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
           QNFTEMNKLWVRIQLQGPA LREKQEKER+ELRDLVGKNLHVLSQIEGV+LEMYKQ+VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYA SNADVIP+FLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDYVDQILGACVK LSSQPKLED RATKQIV+LLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           HLDNATNKVMAMVIIQSIM +NSCISTADKVDVLFELIKGLIKDLEE++VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVARLLHMLHNDDP EMLKIIC VKKHILCGGP+RLPFTVPPLIFSAL+LIR+LQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
           D DVVGEEQ E GD  EEMHATPKKIFQ+LNQLIEALSSVPAPELALRLY+ECAEAANDC
Sbjct: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYMECAEAANDC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           DLE VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQ+MNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQC+AVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGS G
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
           P+TLFVEILNKYL+YFEKGNQQITNAAIQGLIELI TEL TD+PSANP +  DAFFTSTL
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELPTDTPSANPPS--DAFFTSTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 841
           RYIQFQKQKGGVMGERYDSIK+
Sbjct: 781 RYIQFQKQKGGVMGERYDSIKV 800

BLAST of Chy4G071210 vs. TAIR 10
Match: AT1G75850.1 (VPS35 homolog B )

HSP 1 Score: 1272.3 bits (3291), Expect = 0.0e+00
Identity = 638/798 (79.95%), Postives = 721/798 (90.35%), Query Frame = 0

Query: 44  GIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRYYELY 103
           G+EDE+KWLAEGIAGIQHNAF+MH+A+DAN+LRE LKYSA MLSELRTSKLSP +YY+LY
Sbjct: 6   GVEDEDKWLAEGIAGIQHNAFFMHRALDANNLREVLKYSALMLSELRTSKLSPQKYYDLY 65

Query: 104 MRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKEVPAK 163
           MRAFD+LR LEIFFKDESRHG+ VVDLYELVQHAGNILPR+YLLCTVGSVY+K+K+ P+K
Sbjct: 66  MRAFDQLRQLEIFFKDESRHGLPVVDLYELVQHAGNILPRMYLLCTVGSVYIKSKQAPSK 125

Query: 164 EVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVLQNFT 223
           +VLKDLVEMCRGVQHPIRGLFLR YLAQVSRD L +I S+ EGDA+TVM+AVEFVLQNFT
Sbjct: 126 DVLKDLVEMCRGVQHPIRGLFLRSYLAQVSRDKLPEIGSDYEGDANTVMDAVEFVLQNFT 185

Query: 224 EMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLPRVLE 283
           EMNKLWVRIQ QGP  +REKQEKER+ELRDLVGKNLHVL QIEGV+LEMYK+TVLPRVLE
Sbjct: 186 EMNKLWVRIQHQGPGTVREKQEKERNELRDLVGKNLHVLGQIEGVDLEMYKETVLPRVLE 245

Query: 284 QVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMDRLSN 343
           QVVNCKD+LAQYYLMECIIQVFPDEYHLQTLETLL  C QL  TVD ++VL+QLMDRLSN
Sbjct: 246 QVVNCKDKLAQYYLMECIIQVFPDEYHLQTLETLLAACTQLMPTVDTKIVLTQLMDRLSN 305

Query: 344 YAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLD 403
           YA S+ DV+ EFL VEAFAKLSNAIGKVI+ Q++MPIVGA+TL+VSLLTFTLRVHPDRLD
Sbjct: 306 YAASSPDVLHEFLQVEAFAKLSNAIGKVIDTQLEMPIVGAMTLFVSLLTFTLRVHPDRLD 365

Query: 404 YVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMDHLDN 463
           YVDQ+LGACV KLSS PKLEDARA KQ+V LLSAPLEKY+ IVTALTLSNYPRVMDHLD+
Sbjct: 366 YVDQVLGACVVKLSSVPKLEDARAMKQVVALLSAPLEKYSDIVTALTLSNYPRVMDHLDD 425

Query: 464 ATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKEEQNS 523
            TNKVMAM+IIQSIMK +SCISTADKV+VLFELIKGLIKDL+E + +ELDEEDF+EEQNS
Sbjct: 426 GTNKVMAMLIIQSIMKTDSCISTADKVEVLFELIKGLIKDLDETNAEELDEEDFQEEQNS 485

Query: 524 VARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQDGDV 583
           VARL+HML N++P EMLKIIC+V++H++ GGP RLPFTVPPL+FSA+ L+RQL++Q GD+
Sbjct: 486 VARLIHMLDNEEPEEMLKIICVVRRHLMTGGPRRLPFTVPPLVFSAVRLVRQLESQGGDI 545

Query: 584 VGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDCDLEH 643
            GE+            ATP+KIFQILNQ IE L+SVP PELALRLYL+CAEAA+DCDLE 
Sbjct: 546 AGEDS-----------ATPRKIFQILNQTIEVLTSVPCPELALRLYLQCAEAASDCDLEP 605

Query: 644 VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYSAKLL 703
           VAYEFFTQAF++YEE+IADSKAQVTAIHLI+GTLQR+NVFGVENRDTLTHKATGYSA+LL
Sbjct: 606 VAYEFFTQAFMLYEEEIADSKAQVTAIHLIVGTLQRINVFGVENRDTLTHKATGYSARLL 665

Query: 704 KKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSGPVTL 763
           KKPDQCRAVYACSHLFWVDDP+GIKDGERVLLCL+RALRIANAAQQMA+ TRGSSGPVTL
Sbjct: 666 KKPDQCRAVYACSHLFWVDDPDGIKDGERVLLCLRRALRIANAAQQMASATRGSSGPVTL 725

Query: 764 FVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTLRYIQ 823
           FVEILNKY+Y+FEKGN  IT + IQ LIELI  E+Q+D  + N     D FFTSTLRYI+
Sbjct: 726 FVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSD--NGNTTIHSDPFFTSTLRYIK 785

Query: 824 FQKQKGGVMGERYDSIKL 842
           F KQKGG+MGE+YD IKL
Sbjct: 786 FIKQKGGLMGEKYDPIKL 790

BLAST of Chy4G071210 vs. TAIR 10
Match: AT2G17790.1 (VPS35 homolog A )

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 556/802 (69.33%), Postives = 679/802 (84.66%), Query Frame = 0

Query: 40  MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 99
           M++ G EDEEKWLA G A  + NAFYM +A+D+N+L++ LKYSAQMLSELRTSKLSPH+Y
Sbjct: 1   MIADGSEDEEKWLAAGAAAFKQNAFYMQRAIDSNNLKDALKYSAQMLSELRTSKLSPHKY 60

Query: 100 YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKE 159
           Y+LYMRAFDELR LEIFF +E+R G +V++LYELVQHAGNILPRLYLLCT GSVY+K KE
Sbjct: 61  YDLYMRAFDELRKLEIFFMEETRRGCSVIELYELVQHAGNILPRLYLLCTAGSVYIKTKE 120

Query: 160 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 219
            PAKE+LKDLVEMCRG+QHP+RGLFLR YLAQ+SRD L DI SE EGDADTV++AVEFVL
Sbjct: 121 APAKEILKDLVEMCRGIQHPLRGLFLRSYLAQISRDKLPDIGSEYEGDADTVIDAVEFVL 180

Query: 220 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 279
            NFTEMNKLWVR+Q QGPAR +E++EKER ELRDLVGKNLHVLSQ+EGV+L+MY+ TVLP
Sbjct: 181 LNFTEMNKLWVRMQHQGPAREKERREKERGELRDLVGKNLHVLSQLEGVDLDMYRDTVLP 240

Query: 280 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 339
           RVLEQ+VNC+DE+AQYYL++CIIQVFPDEYHLQTL+ LLG CPQLQA+VDI  VLS+LM+
Sbjct: 241 RVLEQIVNCRDEIAQYYLIDCIIQVFPDEYHLQTLDVLLGACPQLQASVDIMTVLSRLME 300

Query: 340 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 399
           RLSNYA  NA+V+P FL VEAF+KL+NAIGKVIEAQ DMPI+ A+TLY SLL FTL VHP
Sbjct: 301 RLSNYAALNAEVLPYFLQVEAFSKLNNAIGKVIEAQEDMPILSAVTLYSSLLKFTLHVHP 360

Query: 400 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 459
           DRLDY DQ+LG+CVK+LS + K++D RATK++V LLSAPLEKYN +VTAL L+NYP V++
Sbjct: 361 DRLDYADQVLGSCVKQLSGKGKIDDTRATKELVSLLSAPLEKYNDVVTALKLTNYPLVVE 420

Query: 460 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKE 519
           +LD  T ++MA VI++SIMKNN+ I+TA+KV+ LFELIKG+I DL+E    E+DE+DF+E
Sbjct: 421 YLDTETKRIMATVIVRSIMKNNTLITTAEKVEALFELIKGIINDLDEPQGLEVDEDDFQE 480

Query: 520 EQNSVARLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 579
           EQNSVA L+HML+NDDP EM KI+ ++KKH L GGP RL FT+PPL+ S L LIR+L  +
Sbjct: 481 EQNSVALLIHMLYNDDPEEMFKIVNVLKKHFLTGGPKRLKFTIPPLVVSTLKLIRRLPVE 540

Query: 580 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 639
             +  G          +E   T  KIFQ LNQ+IEAL +VP+P+LA RLYL+CAEAA+ C
Sbjct: 541 GDNPFG----------KEASVTATKIFQFLNQIIEALPNVPSPDLAFRLYLQCAEAADKC 600

Query: 640 DLEHVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 699
           D E +AYEFFTQA+I+YEE+I+DSKAQVTA+ LIIGTLQRM VFGVENRDTLTHKATGY+
Sbjct: 601 DEEPIAYEFFTQAYILYEEEISDSKAQVTALQLIIGTLQRMQVFGVENRDTLTHKATGYA 660

Query: 700 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 759
           AKLLKKPDQCRAVYACSHLFW++D E I+DGERVLLCLKRAL+IAN+AQQ+AN  RGS+G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWLEDRETIQDGERVLLCLKRALKIANSAQQVANTARGSTG 720

Query: 760 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTL 819
            VTLF+EILNKYLY++EKG  QIT  +++ LI+LI  E   +S  ++P+   ++FF +TL
Sbjct: 721 SVTLFIEILNKYLYFYEKGVPQITVESVESLIKLIKNE---ESMPSDPSA--ESFFATTL 780

Query: 820 RYIQFQKQKGGVMGERYDSIKL 842
            +++FQKQK G +GERY +IK+
Sbjct: 781 EFMEFQKQKEGAIGERYQAIKV 787

BLAST of Chy4G071210 vs. TAIR 10
Match: AT3G51310.1 (VPS35 homolog C )

HSP 1 Score: 1047.3 bits (2707), Expect = 6.5e-306
Identity = 526/803 (65.50%), Postives = 656/803 (81.69%), Query Frame = 0

Query: 46  EDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRYYELYMR 105
           +D+EKWLA  IA ++ NAFYM +A+D+N+L++ LK+SAQMLSELRTSKLSPH+YYELYMR
Sbjct: 4   DDDEKWLAAAIAAVKQNAFYMQRAIDSNNLKDALKFSAQMLSELRTSKLSPHKYYELYMR 63

Query: 106 AFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKAKEVPAKEV 165
            F+EL  LEIFFK+E+  G ++ +LYELVQHAGNILPRLYLLCT+GSVY+K+K+V A ++
Sbjct: 64  VFNELGTLEIFFKEETGRGCSIAELYELVQHAGNILPRLYLLCTIGSVYIKSKDVTATDI 123

Query: 166 LKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVLQNFTEM 225
           LKDLVEMCR VQHP+RGLFLR YLAQV+RD L  I S+ EGD D  M A+EFVLQNFTEM
Sbjct: 124 LKDLVEMCRAVQHPLRGLFLRSYLAQVTRDKLPSIGSDLEGDGDAHMNALEFVLQNFTEM 183

Query: 226 NKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLPRVLEQV 285
           NKLWVR+Q QGP+R +EK+EKER+ELRDLVGKNLHVLSQ+EGV+L +Y+ TVLPR+LEQV
Sbjct: 184 NKLWVRMQHQGPSREKEKREKERNELRDLVGKNLHVLSQLEGVDLGIYRDTVLPRILEQV 243

Query: 286 VNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMDRLSNYA 345
           VNCKDELAQ YLM+CIIQVFPD++HLQTL+ LLG CPQLQ +VDI+ VLS LM+RLSNYA
Sbjct: 244 VNCKDELAQCYLMDCIIQVFPDDFHLQTLDVLLGACPQLQPSVDIKTVLSGLMERLSNYA 303

Query: 346 VSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLDYV 405
            S+ + +P FL VEAF+KL+ AIGKV+EAQ D+P   ++TLY+ LL FTL V+ DRLDYV
Sbjct: 304 ASSVEALPNFLQVEAFSKLNYAIGKVVEAQADLPAAASVTLYLFLLKFTLHVYSDRLDYV 363

Query: 406 DQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMDHLDNAT 465
           DQ+LG+CV +LS+  KL D +A KQIV  LSAPLEKYN +VT L L+NYP VM++LD  T
Sbjct: 364 DQVLGSCVTQLSATGKLCDDKAAKQIVAFLSAPLEKYNNVVTILKLTNYPLVMEYLDRET 423

Query: 466 NKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEELSVDELDEEDFKEEQNSVA 525
           NK MA++++QS+ KNN+ I+TAD+VD LFEL KGL+KD +    DE+DEEDF+EEQN VA
Sbjct: 424 NKAMAIILVQSVFKNNTHIATADEVDALFELAKGLMKDFDGTIDDEIDEEDFQEEQNLVA 483

Query: 526 RLLHMLHNDDPGEMLKIICIVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQDGDVVG 585
           RL++ L+ DDP EM KII  V+KHI+ GGP RLP T+PPL+FSAL LIR+L+       G
Sbjct: 484 RLVNKLYIDDPEEMSKIIFTVRKHIVAGGPKRLPLTIPPLVFSALKLIRRLR-------G 543

Query: 586 EEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDCDLEHVA 645
            +++  GD+A    ATPK+I Q+L++ +E LS V AP+LALRLYL+CA+AAN+C+LE VA
Sbjct: 544 GDENPFGDDAS---ATPKRILQLLSETVEVLSDVSAPDLALRLYLQCAQAANNCELETVA 603

Query: 646 YEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYSAKLLKK 705
           YEFFT+A+++YEE+I+DSKAQVTA+ LIIGTLQRM VF VENRDTLTHKATGYSA+LL+K
Sbjct: 604 YEFFTKAYLLYEEEISDSKAQVTALRLIIGTLQRMRVFNVENRDTLTHKATGYSARLLRK 663

Query: 706 PDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRG--SSGPVTL 765
           PDQCRAVY C+HLFW D+ E +KDGERV+LCLKRA RIA+A QQMAN +RG  S+G V+L
Sbjct: 664 PDQCRAVYECAHLFWADECENLKDGERVVLCLKRAQRIADAVQQMANASRGTSSTGSVSL 723

Query: 766 FVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPATAPDAFFTSTLRYIQ 825
           +VE+LNKYLY+ EKGNQQ+T   I+ L ELI +E +     A P      F  STLRYI+
Sbjct: 724 YVELLNKYLYFLEKGNQQVTGDTIKSLAELIKSETKKVESGAEP------FINSTLRYIE 783

Query: 826 FQK-QKGGVMGERYDSIKLEGME 846
           FQ+ Q+ G M E+Y+ IK+E  E
Sbjct: 784 FQRQQEDGGMNEKYEKIKMEWFE 790