Chy4G070280 (gene) Cucumber (hystrix) v1

Overview
NameChy4G070280
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionreplication protein A 32 kDa subunit A-like
LocationchrH04: 2755800 .. 2760458 (+)
RNA-Seq ExpressionChy4G070280
SyntenyChy4G070280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTCCAGTACCCAGTTTGATTCCGCCAGCGGATTTACGTCTTCTCAGACCAATGACTCCTCTTCAGCGAAGGTAAGTCGCTGCTTCAACTTCTAAATTTCTGCTTGATCCAGAGGGATTCTCTTTTAATTAATGAATAGAATTGGGCTTCAAGGAACCGGTACTCCTTATAAATTCGTTGGATGGCTAGAATCCAGCGAGAGCAAAACTGAAAGCGTAAACCTAAATCGTTGTTAGATCACCGGAACGATGATCGGAACGGTGTTGAAATTTCAAGAAATGGGGTTCGAATTCATTTTATTTATAGTCATATTTCTGGGTGTAGACCAATTTTTGTATGTCGGATAAATCTTGAGAGAATCGTGAATAAAATGCTAATTGTCGGATGTTTGTTACATTTTCTTCATATCTTTTTAATTATTCCATGCAGAGTCGAGAATCCCCGGGCTTGGTTCCGGTAACTGTGAAACAGATCAGCGAAGCTTCACATTCTGGCGAAGAGAAGGCCAACTTTGTAATCAACGGCGTTGATATTACAAATGTATATTTCCTTGTCTTCTCTCCAACTGTTATGTATTATTTAAAAACGACAACAAGAAAACGATGTAGATGGTCTTCTGTTAATATTTTTGGGTGGTTTAGTTATTCTTAGAGTCTTTAACCCTTCAACCTTAAGAAGCATGGACATTACGTTAGCAGAGTGTTGAATTCTGCAATGCGCAGGGAAGGTTGTGGATTTCAGTTTTTTTACATCATATAAATGTTAATTGAAATAAACAGTTGTCAAATCATAGAAATTTTCTACAGTAAAATTGTATAATCAACATGTAACTTTTCCCCTCGTGACAAGCATCGTTTCTTAAAAGGCCGTGTTGGTGTACATGTTCATGATTGGCTCCTCCAGATTTGATCATCAACCTGTGTATTTCTATCAAGTTTTTAAGTTCGAGTTTCTCATGCTGTACTTGTAAATTCTCTTTTGTTACAAGTACTGACATTTTAGTTATTGAGAAAAGGTTACAATCGTTGGGAAGGTCTCTGAGAAAGCTGAAAGGAATACGGATATCACATTCACTGTAGACGATGGAACAGGAACAATTGGTTGCAAAAGATGGTATACTGTTGCAATTGCAATCTTCCTAAACCTTTTAACTAATCTTCATTCTAGCGTTGTATCTGTTCTGTTTTACTGAAGAATGTGTGTCAAATTTTATTCAGGGTAAACGACACTTTTGACACCAACCAAATGCAGGAAATACAGTAAGTTCTTATCTAGTTTGATTCGCTTGTGATGAAAAATACTTTAGTGGTCTTGATTCTCATATAGTTTGATTTTTCATGGCACAGAGATGGAATGTACGTTCGGGTGAATGGGCATTTGAAAATGTTCCAAAGCAATAAGCAAATATTTGCATTCTCCGTAAGGTATGGTATTATGATTCTCTTCAATCTATTACATGCCTTTGGGATCTCTTTTGAAGTTAAATGTTTTACACATAAGGTTATCATTCTGCTCGGTGTTTTATTGCATGTTGAAATTTTAGGATTCACACATCAAGTAAGTTGAAGTAGGTCAATGTTAGCTAAGCACCGTTCTGGAAAGATGCCATATATGTTCTAATTCATTGTTGTAGTAAAACCAGCAACTATACAACTACAACCTTGCTTGAATAGGATTGAATGGAATCCGTTCTATTGTGCCAATTCTCAGTTCTTACATCTATTGCAGCAAATATAGTCTACTGGTTTTGTAAGTAGAACAACCAGGATATGTTACAGTGCCCTTGAGTTCTGTGTGATTGTCACGGAGAGAGGTACTCGGTATTGGTACGAAGGAATTAAGGTCCAACAGAAGAAATGGGTAGTGTTGGTGCCGAAAGACCAGTTTTATTCACATTTTACAAAGAAGTGAAGGTGGCCATTATATTTGTTTCTAATCATAGTGCTCGTAAACTTTCAAACTAAGCATATCAGGATAACAAAATTTGTTTTTGAAGTGTTTTCATTAGGATCTCTACTTTGTTGAAGGTCTCGGGGATGTGTCAGGTGCAGAGTAATATTTTATGCTTTGAAAGTTAATATTTTAAATTCTTAAACTTCTTTCTCAATGAATTTAAGAAATTTTAAGAATAGTATTAACTTCTCACGTTGCCAGTGCATGGGCACTATGAGACGTGTCATTTGTTAGTTTTGACATAGTAGTGGAATTAGATCAATTCCATATCAACCCTCGACTTAAAATCAATTATTAGTGGCTAAATAGTTAAAACTATTTCTAGATTACATGCTTTGAATGATGACATTTACATATGGGATTTAGCAAATTTTCGCCAGTGTTGGTTTAAGAAATGTCATGTTCTCCCTTAAAATTTACAGTAGCTGATTTCCTTTCTGGATTTCCCCAATTAGTAAAATATGAACAAGCAAACCTCTGGGAAACTAATGTCTTGTTAATGCTATATAGTCTCTCTTTGTTAATACGTAATTGCCTAAAACTTCTGCTTGAAACTACAGTCCAAAGGATTGATTTTTTCCCAAGCTTGGTCACTAGGCAGTGACCAATATCAAGTGTAATTATTTGGATATTGGAAACCGTCTAATACCAAATAATCTGTCCCTGTTGTATATTTGATCAACTGACACGATGATTGAATTTGGTTATGCTTTGCCAGGCCTGTTACGAACTTTGATGAAATTACCTTCCACTTTATTGAATGCATACACGACCACTTGCGGAACTCCAAGTTACAGGTACTGGTTCCTGGAGCTGACTTGGTAGTTGTGATTCGAACTTGTAACTTTGTTTTTTCTATAGTCTAACGGAATTAATTATTAACGAATATCATAATTCTCAATTCATTTGTCTTAAACTGCTAGAATCTCAAAGGCAATGGATCAACACAGCTCCAAACCTCAGATTCAATTGTGAAGACTCCAGTGCAGAATGGCTCGAATGGATATCATACAACTTCTTCAGCAATAGTAAGTCTTAATTAACCATGATAATTATTGAGAATGTGACAAAAATATCACACGACTGTAAATAGAAGATTTAGAATATGCAAGTGAGGAATAATATATCATATTGATGTCCTTCGCGTGAAGCTAAAAGTAAAGTAACGTGAGTTGGATCTAAAGTAAACAATATCATATTATTGTAGAGATGTGTGGAGGGTTGTCTTTAACAGTTATGACTGTGCATCTATATCTTATCGGGGATTGCTTGATAGGAATTTAAATCTGATTTCTTTTTGCAGCCATCTGAGCAGCACACTGTTGATGTTAAAAAGAGCTGTGATGAATTGGTTCTTGACTACCTGCAGCTGCCCTCCAGCGTGTAAGTTGTCCCAAACCAAAGTTTGATTTTTCTTTGCAGATTGTTTTGTTTGTTGGCTAATTACTGTGCGTTTTCCTATATGAAACTGACTTAAGCTTGCCGGACACAAGGTGTTTTTACATTTAAATAATTGAGCTCTATCATCAGATGTGATTTTGAGTCATTCGTGGTACTTTTTAACTTCTGTGAATAGAAAACAAGACGTGAGGTCGGTGGTTGACTGTTAGTCAGGTCACTTCTGATTATTGGTTCAATAGACACTCACACTGAGTTGACCCTTGGCTCAATAATAAGGGGGGACAAGATATGACTTTAGGTTGCACGTTTCAATTTTCATGTGGGCCACTAAAAAAAAAACCCAACTAATTATTGTAGATTAGAAATTTGGTATTGCCCTTGAGATTTATTGTTGCCCAAGTATAGGACAATAATTTCAGATGGATTGTTCCGATGCACGTAAACTGGCTGGAACACTTATGGATATAAAAAAAAAGTATAGAACAATGATTATATGCAATCCTTTGTTTTGTTACCTAGTAAAAGGTATATGATATTTGTTTTTTTTTAAATGTCACCTTACAAATTTTTATTGGACAACTATTTGAGCTACAGGTAGATGCAAATTAGATTGCACCTTAAGTTTTTTTTAGATAAATATAGGTTATGACATGATTTTGGTTTATGAACTTATTTTAATTCCTTTTACTTTCAAATGTATTTAGTTTTTTGCACTATCAATATCTTAAATGATTTGTATTTGATGTTAGTTTTCATCGAAGTTTGTTAATTAAATAGAGTGAACGAGACTAAAACTTTGAGATGACATTTGAGATTTATCTATATACAGACTAAATTTGGATATCTTAGAGTACATGGACTTAAGGGTTTTTGTACGTATAATTTGATTCATCTTTAGAGATGTTATTTTATTTATTTGACAATTAATTATAATTTAGTAAAATGATCAAGAGTTAAAAAAATTGTTCTATGAAACTAATGTATAAACTACTTATTCTGGTACAAGGGGAAAAATGGCTTCTTTTACTTGGAGAAGTTAATGTTTTTTTTCTTTCTTTTCAGTGCAAAAGAAAGAGGAATACACAAAGATGAACTTTCCCAACAACTGAAAATTCCAATGGAGAAAATACTGTATGTTTCACCTACCTTCCAACTCCCTTGTGTGGTTAATATGCAAATTAACTTATATTTACTTTTTTGTTTTCTTTTGGTTTGGTTTTAGGGATTCCATAAGGTCTCTTGAAGATGAAGGTTTGATATATTCCACCATAGATGAGTTTCATTTCAAGTCTGCTGCATAG

mRNA sequence

ATGTTCTCCAGTACCCAGTTTGATTCCGCCAGCGGATTTACGTCTTCTCAGACCAATGACTCCTCTTCAGCGAAGAGTCGAGAATCCCCGGGCTTGGTTCCGGTAACTGTGAAACAGATCAGCGAAGCTTCACATTCTGGCGAAGAGAAGGCCAACTTTGTAATCAACGGCGTTGATATTACAAATGTTACAATCGTTGGGAAGGTCTCTGAGAAAGCTGAAAGGAATACGGATATCACATTCACTGTAGACGATGGAACAGGAACAATTGGTTGCAAAAGATGGGTAAACGACACTTTTGACACCAACCAAATGCAGGAAATACAAGATGGAATGTACGTTCGGGTGAATGGGCATTTGAAAATGTTCCAAAGCAATAAGCAAATATTTGCATTCTCCGTAAGGCCTGTTACGAACTTTGATGAAATTACCTTCCACTTTATTGAATGCATACACGACCACTTGCGGAACTCCAAGTTACAGAATCTCAAAGGCAATGGATCAACACAGCTCCAAACCTCAGATTCAATTGTGAAGACTCCAGTGCAGAATGGCTCGAATGGATATCATACAACTTCTTCAGCAATACCATCTGAGCAGCACACTGTTGATGTTAAAAAGAGCTGTGATGAATTGGTTCTTGACTACCTGCAGCTGCCCTCCAGCGTTGCAAAAGAAAGAGGAATACACAAAGATGAACTTTCCCAACAACTGAAAATTCCAATGGAGAAAATACTGGATTCCATAAGGTCTCTTGAAGATGAAGGTTTGATATATTCCACCATAGATGAGTTTCATTTCAAGTCTGCTGCATAG

Coding sequence (CDS)

ATGTTCTCCAGTACCCAGTTTGATTCCGCCAGCGGATTTACGTCTTCTCAGACCAATGACTCCTCTTCAGCGAAGAGTCGAGAATCCCCGGGCTTGGTTCCGGTAACTGTGAAACAGATCAGCGAAGCTTCACATTCTGGCGAAGAGAAGGCCAACTTTGTAATCAACGGCGTTGATATTACAAATGTTACAATCGTTGGGAAGGTCTCTGAGAAAGCTGAAAGGAATACGGATATCACATTCACTGTAGACGATGGAACAGGAACAATTGGTTGCAAAAGATGGGTAAACGACACTTTTGACACCAACCAAATGCAGGAAATACAAGATGGAATGTACGTTCGGGTGAATGGGCATTTGAAAATGTTCCAAAGCAATAAGCAAATATTTGCATTCTCCGTAAGGCCTGTTACGAACTTTGATGAAATTACCTTCCACTTTATTGAATGCATACACGACCACTTGCGGAACTCCAAGTTACAGAATCTCAAAGGCAATGGATCAACACAGCTCCAAACCTCAGATTCAATTGTGAAGACTCCAGTGCAGAATGGCTCGAATGGATATCATACAACTTCTTCAGCAATACCATCTGAGCAGCACACTGTTGATGTTAAAAAGAGCTGTGATGAATTGGTTCTTGACTACCTGCAGCTGCCCTCCAGCGTTGCAAAAGAAAGAGGAATACACAAAGATGAACTTTCCCAACAACTGAAAATTCCAATGGAGAAAATACTGGATTCCATAAGGTCTCTTGAAGATGAAGGTTTGATATATTCCACCATAGATGAGTTTCATTTCAAGTCTGCTGCATAG

Protein sequence

MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDITNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHLKMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKTPVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKIPMEKILDSIRSLEDEGLIYSTIDEFHFKSAA*
Homology
BLAST of Chy4G070280 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 250.4 bits (638), Expect = 2.4e-65
Identity = 136/281 (48.40%), Postives = 193/281 (68.68%), Query Frame = 0

Query: 1   MFSSTQFD-----SASGFTSSQ-----TNDSSSAKSRESPGLVPVTVKQISEASHSGEEK 60
           MFSS+QF+     S  GF SSQ      + SS+AK+R+  GLVPVTVKQI+E   S  EK
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  ANFVINGVDITNVTIVGKVSEKAE-RNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQ 120
           +  VING+ +TNV++VG V +K E + T++ FT+DDGTG I CKRWV++TFD  +M+ ++
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVNGHLKMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGST 180
           DG YVR++GHLK FQ   Q+  FSVRP+ +F+E+TFH+IECIH + +NS+ Q  +    T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 181 QLQTSDSIVKTPVQNGSNGYHTT--SSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKER 240
           Q       V T  Q GSN    T  +  + S+ +  + +K+ D+++LDYL+ P+  A+++
Sbjct: 181 Q------SVNTTFQGGSNTNQATLLNPVVSSQNNDGNGRKNLDDMILDYLKQPACTARQQ 240

Query: 241 GIHKDELSQQLKIPMEKILDSIRSLEDEGLIYSTIDEFHFK 269
           GIH DE++QQLKIP  K+   ++SLE +GLIYSTIDE+HFK
Sbjct: 241 GIHIDEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

BLAST of Chy4G070280 vs. ExPASy Swiss-Prot
Match: Q6K9U2 (Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2A PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.1e-46
Identity = 117/287 (40.77%), Postives = 165/287 (57.49%), Query Frame = 0

Query: 2   FSSTQFDSASGFTSSQ---TNDSSSAKSRESPGLVPVTVKQISEASHS---GEEKANFVI 61
           FS     S S FTSSQ    + ++ +KSR +   +P+TVKQISEA  S   GE+ A FV+
Sbjct: 4   FSQPDAFSPSQFTSSQNAAADSTTPSKSRGASSTMPLTVKQISEAQQSGITGEKGAPFVV 63

Query: 62  NGVDITNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVR 121
           +GV+  NV +VG VS K ERNTD++FT+DDGTG +   RWVND  D+ +   +Q+GMYV 
Sbjct: 64  DGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAAVQNGMYVS 123

Query: 122 VNGHLKMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQ------------NL 181
           V G LK  Q  K+  AF++RPVT+++E+T HFI+C+  HL N+K Q            + 
Sbjct: 124 VIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENTKSQIGSPAKTYSAMGSS 183

Query: 182 KGNGSTQLQTSDSIVKTPVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSV 241
             NG +++ T  S+   P               P    T   K   +  VL+  + P++V
Sbjct: 184 SSNGFSEMTTPTSVKSNPA--------------PVLSVTNGSKTDLNTEVLNVFREPANV 243

Query: 242 AKERGIHKDELSQQLKIPMEKILDSIRSLEDEGLIYSTIDEFHFKSA 271
             E G+H DE+ ++ ++P  KI  +I  L D G IYSTIDE H+KSA
Sbjct: 244 ESEHGVHIDEIVKRFRLPEAKIKVAIDYLADIGHIYSTIDESHYKSA 276

BLAST of Chy4G070280 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.6e-45
Identity = 106/271 (39.11%), Postives = 165/271 (60.89%), Query Frame = 0

Query: 9   SASGFTSSQT-----NDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDITNV 68
           +  GF  SQ        SSS K+R+   L+P+T+KQ+S AS +GE  +NF I+GVDI  V
Sbjct: 13  AGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSASTTGE--SNFSIDGVDIKTV 72

Query: 69  TIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHLKMF 128
            IVG++S    R T + F VDDGTG + C RW +   +T +M+ ++ GMYVR++GHLK+F
Sbjct: 73  VIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLGMYVRLHGHLKIF 132

Query: 129 QSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQN---LKGNGSTQLQTSDSIVKT 188
           Q  + +  FSVRPVT+F+EI  HF EC++ H+ N+KL+     +   + + Q   S + T
Sbjct: 133 QGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTATPRPQMPYSTMPT 192

Query: 189 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 248
           P +    G    S+  P++ +  D      + VL+YL  P  +  E G+H D ++++L+I
Sbjct: 193 PAKPYQTG---PSNQFPNQFN--DSMHGVKQTVLNYLNQPMHIVSEAGVHCDIIARELRI 252

Query: 249 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 272
           P+ ++ +++  L ++G IYST+DE  FKS A
Sbjct: 253 PLLQVKEALEQLSNDGCIYSTLDETCFKSTA 276

BLAST of Chy4G070280 vs. ExPASy Swiss-Prot
Match: Q6H7J5 (Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2B PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.1e-41
Identity = 94/267 (35.21%), Postives = 158/267 (59.18%), Query Frame = 0

Query: 12  GFTSSQTNDSSSA---------KSRESPGLVPVTVKQISEASHSGEEKANFVINGVDITN 71
           GF  SQ  +++           KSR +  L+P+TVKQI +AS + ++K+NF +NG++++ 
Sbjct: 29  GFMPSQATNAAEGTSGGGGGFPKSRNAQALLPLTVKQIMDASQTNDDKSNFAVNGMEVST 88

Query: 72  VTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHLKM 131
           V +VG++  K +R TD++FT+DDGTG +   RW ND+ DT +M +IQ+G YV VNG LK 
Sbjct: 89  VRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKEMADIQNGDYVIVNGGLKG 148

Query: 132 FQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKTPV 191
           FQ  +Q+ A+SVR +TNF+++T HF+ C+H HL  ++ ++ + N +T   T +  +    
Sbjct: 149 FQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKS-QVNANTATGTPNQTMPRDS 208

Query: 192 QNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKIPM 251
              +    T  ++  S         +  +LVL+    P+ +  + G+  D +S++L +P 
Sbjct: 209 MAYNQSPLTNQASTFSAPQNTGTGTNMIDLVLNVFHDPAVMNDDHGVGVDYVSRRLNLPE 268

Query: 252 EKILDSIRSLEDEGLIYSTIDEFHFKS 270
           E +   I    D G +Y+TID+ H+KS
Sbjct: 269 ETVGKIIIDQVDLGHLYATIDDHHYKS 294

BLAST of Chy4G070280 vs. ExPASy Swiss-Prot
Match: Q5Z8L1 (Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2C PE=1 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.0e-23
Identity = 65/160 (40.62%), Postives = 92/160 (57.50%), Query Frame = 0

Query: 9   SASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEA-----SHSGEEKANFVINGVDITNV 68
           SA+G  ++     S  +     G VP TV  IS +     +  G     F I+GV+ TNV
Sbjct: 27  SAAGTGAAAAPSPSKPRDPRFSGCVPATVLHISRSFAAALAADGGGDPVFSIDGVETTNV 86

Query: 69  TIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHLKMF 128
            ++G+V     R+TD+ FT+DD TG I   RW+ D  DT     IQ+G+YV+V  +L  F
Sbjct: 87  RVLGRVVSVVSRDTDVCFTLDDSTGKIPLVRWITDQSDTRDTSYIQEGVYVKVQVNLMGF 146

Query: 129 QSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRN--SKLQ 162
           Q+ KQ  A S+RP+ NF+E+  HFIEC+H HL +  SK+Q
Sbjct: 147 QAKKQGLARSIRPINNFNEVVLHFIECMHVHLESVQSKMQ 186

BLAST of Chy4G070280 vs. ExPASy TrEMBL
Match: A0A0A0LEG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881710 PE=3 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 6.3e-146
Identity = 268/271 (98.89%), Postives = 271/271 (100.00%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSSTQFDSASGFTSSQTNDSSSAKSRESPGL+PVTVKQISEASHSGEEKANFVINGVDI
Sbjct: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLIPVTVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQM+EIQDGMYVRVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMEEIQDGMYVRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT
Sbjct: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLK+
Sbjct: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKL 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 272
           PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. ExPASy TrEMBL
Match: A0A1S3CR64 (replication protein A 32 kDa subunit A-like OS=Cucumis melo OX=3656 GN=LOC103503766 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.5e-142
Identity = 263/271 (97.05%), Postives = 268/271 (98.89%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSSTQFDS SGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVIN VDI
Sbjct: 1   MFSSTQFDSGSGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINDVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDT QM+EIQDGMYVRVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTKQMEEIQDGMYVRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           KMFQSNKQIFAFSVRPVTNFDEITFHF+ECIHDHLRNSKLQNLKG+GSTQLQTSDSIVKT
Sbjct: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFLECIHDHLRNSKLQNLKGDGSTQLQTSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           PVQNGS+GYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIH+DELSQQLKI
Sbjct: 181 PVQNGSSGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHRDELSQQLKI 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 272
           PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. ExPASy TrEMBL
Match: A0A6J1F8S8 (replication protein A 32 kDa subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC111443128 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.8e-133
Identity = 243/271 (89.67%), Postives = 259/271 (95.57%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSS+QFDS SGFTSSQTNDSSSAK+RE+PGLVP TVKQISEASHSGEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTNDSSSAKNRETPGLVPATVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITF VDDGTG IGCKRWVNDTFDT QM EIQDGMY+RVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           K+FQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQNL G GSTQ Q+SDSIVKT
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           P+QNGS+GY T SS +PSEQHTVDVKK+CD+L+LDYLQLPSSVAKERGIH+DELSQQLKI
Sbjct: 181 PMQNGSSGYQTASSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQLKI 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 272
           PMEKIL+SIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILNSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. ExPASy TrEMBL
Match: A0A6J1IMU1 (replication protein A 32 kDa subunit A-like OS=Cucurbita maxima OX=3661 GN=LOC111476648 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.3e-130
Identity = 241/273 (88.28%), Postives = 257/273 (94.14%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSS+QFDS SGFT SQTNDSSSAK+RE+PGLVP TVKQISEASHSGEEKANF INGVDI
Sbjct: 1   MFSSSQFDSGSGFTFSQTNDSSSAKNRETPGLVPATVKQISEASHSGEEKANFAINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITF VDDGTG IGCKRWVNDTFDT QM EIQDGMY+RVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           K+FQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQNL G GSTQ Q+SDSIVKT
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 PVQNGSNGYHTTS--SAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQL 240
           P+QNGS+GY T S  S +PSEQHTVDVKK+CD+L+LDYLQLPSSVAKERGIH+DELSQQL
Sbjct: 181 PMQNGSSGYQTASSVSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQL 240

Query: 241 KIPMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 272
           KIPMEKIL+SIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 KIPMEKILNSIRSLEDEGLIYSTIDEFHFKSAA 273

BLAST of Chy4G070280 vs. ExPASy TrEMBL
Match: A0A6J1CUB8 (replication protein A 32 kDa subunit A OS=Momordica charantia OX=3673 GN=LOC111014622 PE=3 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 4.6e-120
Identity = 230/271 (84.87%), Postives = 245/271 (90.41%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSS+QFDS SGFTSSQ  DS+SAKSRESPGLVPVTVKQISEA HSGEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQVPDSTSAKSRESPGLVPVTVKQISEAFHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITF VDDGTG IGCKRWVNDTFDT QM +IQDGMY+RVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFVVDDGTGRIGCKRWVNDTFDTKQMDDIQDGMYIRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           K+FQ N QIFAFSVRPVTN DEITFHFIECIHD+LRNSKLQ L G  STQ Q+SDS V  
Sbjct: 121 KIFQGNMQIFAFSVRPVTNCDEITFHFIECIHDYLRNSKLQ-LNGVASTQSQSSDSTVNA 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
            VQ+GS+GY T SS + S QHTVDVKK+ D+LVLDYLQLPSSVAKE+GIH+DELSQQLKI
Sbjct: 181 LVQSGSSGYQTASSELSSGQHTVDVKKNPDDLVLDYLQLPSSVAKEKGIHRDELSQQLKI 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 272
           P+EKILDSIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PLEKILDSIRSLEDEGLIYSTIDEFHFKSAA 270

BLAST of Chy4G070280 vs. NCBI nr
Match: XP_004136317.1 (replication protein A 32 kDa subunit A [Cucumis sativus] >KGN60158.1 hypothetical protein Csa_001153 [Cucumis sativus])

HSP 1 Score: 526 bits (1356), Expect = 3.12e-188
Identity = 268/271 (98.89%), Postives = 271/271 (100.00%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSSTQFDSASGFTSSQTNDSSSAKSRESPGL+PVTVKQISEASHSGEEKANFVINGVDI
Sbjct: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLIPVTVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQM+EIQDGMYVRVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMEEIQDGMYVRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT
Sbjct: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLK+
Sbjct: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKL 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271
           PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. NCBI nr
Match: XP_008466326.1 (PREDICTED: replication protein A 32 kDa subunit A-like [Cucumis melo])

HSP 1 Score: 515 bits (1327), Expect = 8.23e-184
Identity = 263/271 (97.05%), Postives = 268/271 (98.89%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSSTQFDS SGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVIN VDI
Sbjct: 1   MFSSTQFDSGSGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINDVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDT QM+EIQDGMYVRVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTKQMEEIQDGMYVRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           KMFQSNKQIFAFSVRPVTNFDEITFHF+ECIHDHLRNSKLQNLKG+GSTQLQTSDSIVKT
Sbjct: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFLECIHDHLRNSKLQNLKGDGSTQLQTSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           PVQNGS+GYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIH+DELSQQLKI
Sbjct: 181 PVQNGSSGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHRDELSQQLKI 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271
           PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. NCBI nr
Match: XP_038897330.1 (replication protein A 32 kDa subunit A-like [Benincasa hispida])

HSP 1 Score: 496 bits (1277), Expect = 3.44e-176
Identity = 252/271 (92.99%), Postives = 262/271 (96.68%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFS TQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFV+NGVDI
Sbjct: 1   MFSGTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVLNGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNV IVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDT QM+EIQDGMYVRVNGHL
Sbjct: 61  TNVNIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTKQMEEIQDGMYVRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           K+FQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNL G+G TQ Q+SDSIVKT
Sbjct: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLNGDGPTQFQSSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           PVQNGS+G+ T SS IPSEQHTVDVKK+CDELVLDYLQLPSSVAKERGIH+DELSQQLKI
Sbjct: 181 PVQNGSSGHQTASSGIPSEQHTVDVKKNCDELVLDYLQLPSSVAKERGIHRDELSQQLKI 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271
           PMEKIL+SIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILNSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. NCBI nr
Match: XP_022936562.1 (replication protein A 32 kDa subunit A-like [Cucurbita moschata])

HSP 1 Score: 484 bits (1247), Expect = 1.28e-171
Identity = 243/271 (89.67%), Postives = 259/271 (95.57%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSS+QFDS SGFTSSQTNDSSSAK+RE+PGLVP TVKQISEASHSGEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTNDSSSAKNRETPGLVPATVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITF VDDGTG IGCKRWVNDTFDT QM EIQDGMY+RVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           K+FQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQNL G GSTQ Q+SDSIVKT
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           P+QNGS+GY T SS +PSEQHTVDVKK+CD+L+LDYLQLPSSVAKERGIH+DELSQQLKI
Sbjct: 181 PMQNGSSGYQTASSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQLKI 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271
           PMEKIL+SIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILNSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. NCBI nr
Match: KAG7024504.1 (Replication protein A 32 kDa subunit A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 480 bits (1235), Expect = 8.64e-170
Identity = 241/271 (88.93%), Postives = 257/271 (94.83%), Query Frame = 0

Query: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDI 60
           MFSS+QFDS SGFTSSQT DSSSAK+RE+PGLVP TVKQISEASHSGEEKANF INGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTYDSSSAKNRETPGLVPATVKQISEASHSGEEKANFAINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHL 120
           TNVTIVGKVSEKAERNTDITF VDDGTG IGCKRWVNDTFDT QM EIQDGMY+RVNGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180
           K+FQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQNL G GSTQ Q+SDSIVKT
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 240
           P+QNGS+GY T SS +PSEQHTVDVKK+CD+L+LDYLQLPSSVAKERGIH+DELSQQLKI
Sbjct: 181 PMQNGSSGYQTASSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQLKI 240

Query: 241 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 271
           PMEKIL+SIRSLEDEGLIYSTIDEFHFKSAA
Sbjct: 241 PMEKILNSIRSLEDEGLIYSTIDEFHFKSAA 271

BLAST of Chy4G070280 vs. TAIR 10
Match: AT2G24490.1 (replicon protein A2 )

HSP 1 Score: 250.4 bits (638), Expect = 1.7e-66
Identity = 136/281 (48.40%), Postives = 193/281 (68.68%), Query Frame = 0

Query: 1   MFSSTQFD-----SASGFTSSQ-----TNDSSSAKSRESPGLVPVTVKQISEASHSGEEK 60
           MFSS+QF+     S  GF SSQ      + SS+AK+R+  GLVPVTVKQI+E   S  EK
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  ANFVINGVDITNVTIVGKVSEKAE-RNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQ 120
           +  VING+ +TNV++VG V +K E + T++ FT+DDGTG I CKRWV++TFD  +M+ ++
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVNGHLKMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGST 180
           DG YVR++GHLK FQ   Q+  FSVRP+ +F+E+TFH+IECIH + +NS+ Q  +    T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 181 QLQTSDSIVKTPVQNGSNGYHTT--SSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKER 240
           Q       V T  Q GSN    T  +  + S+ +  + +K+ D+++LDYL+ P+  A+++
Sbjct: 181 Q------SVNTTFQGGSNTNQATLLNPVVSSQNNDGNGRKNLDDMILDYLKQPACTARQQ 240

Query: 241 GIHKDELSQQLKIPMEKILDSIRSLEDEGLIYSTIDEFHFK 269
           GIH DE++QQLKIP  K+   ++SLE +GLIYSTIDE+HFK
Sbjct: 241 GIHIDEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

BLAST of Chy4G070280 vs. TAIR 10
Match: AT2G24490.2 (replicon protein A2 )

HSP 1 Score: 250.4 bits (638), Expect = 1.7e-66
Identity = 136/281 (48.40%), Postives = 193/281 (68.68%), Query Frame = 0

Query: 1   MFSSTQFD-----SASGFTSSQ-----TNDSSSAKSRESPGLVPVTVKQISEASHSGEEK 60
           MFSS+QF+     S  GF SSQ      + SS+AK+R+  GLVPVTVKQI+E   S  EK
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  ANFVINGVDITNVTIVGKVSEKAE-RNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQ 120
           +  VING+ +TNV++VG V +K E + T++ FT+DDGTG I CKRWV++TFD  +M+ ++
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVNGHLKMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGST 180
           DG YVR++GHLK FQ   Q+  FSVRP+ +F+E+TFH+IECIH + +NS+ Q  +    T
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVGDVT 180

Query: 181 QLQTSDSIVKTPVQNGSNGYHTT--SSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKER 240
           Q       V T  Q GSN    T  +  + S+ +  + +K+ D+++LDYL+ P+  A+++
Sbjct: 181 Q------SVNTTFQGGSNTNQATLLNPVVSSQNNDGNGRKNLDDMILDYLKQPACTARQQ 240

Query: 241 GIHKDELSQQLKIPMEKILDSIRSLEDEGLIYSTIDEFHFK 269
           GIH DE++QQLKIP  K+   ++SLE +GLIYSTIDE+HFK
Sbjct: 241 GIHIDEIAQQLKIPKNKLEGVVQSLEGDGLIYSTIDEYHFK 275

BLAST of Chy4G070280 vs. TAIR 10
Match: AT3G02920.1 (Replication protein A, subunit RPA32 )

HSP 1 Score: 184.5 bits (467), Expect = 1.1e-46
Identity = 106/271 (39.11%), Postives = 165/271 (60.89%), Query Frame = 0

Query: 9   SASGFTSSQT-----NDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDITNV 68
           +  GF  SQ        SSS K+R+   L+P+T+KQ+S AS +GE  +NF I+GVDI  V
Sbjct: 13  AGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSASTTGE--SNFSIDGVDIKTV 72

Query: 69  TIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHLKMF 128
            IVG++S    R T + F VDDGTG + C RW +   +T +M+ ++ GMYVR++GHLK+F
Sbjct: 73  VIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLGMYVRLHGHLKIF 132

Query: 129 QSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQN---LKGNGSTQLQTSDSIVKT 188
           Q  + +  FSVRPVT+F+EI  HF EC++ H+ N+KL+     +   + + Q   S + T
Sbjct: 133 QGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTATPRPQMPYSTMPT 192

Query: 189 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKI 248
           P +    G    S+  P++ +  D      + VL+YL  P  +  E G+H D ++++L+I
Sbjct: 193 PAKPYQTG---PSNQFPNQFN--DSMHGVKQTVLNYLNQPMHIVSEAGVHCDIIARELRI 252

Query: 249 PMEKILDSIRSLEDEGLIYSTIDEFHFKSAA 272
           P+ ++ +++  L ++G IYST+DE  FKS A
Sbjct: 253 PLLQVKEALEQLSNDGCIYSTLDETCFKSTA 276

BLAST of Chy4G070280 vs. TAIR 10
Match: AT3G02920.2 (Replication protein A, subunit RPA32 )

HSP 1 Score: 183.7 bits (465), Expect = 1.9e-46
Identity = 100/253 (39.53%), Postives = 159/253 (62.85%), Query Frame = 0

Query: 22  SSAKSRESPGLVPVTVKQISEASHSGEEKANFVINGVDITNVTIVGKVSEKAERNTDITF 81
           SS ++R+   L+P+T+KQ+S AS +GE  +NF I+GVDI  V IVG++S    R T + F
Sbjct: 51  SSIRNRDVRTLLPLTLKQLSSASTTGE--SNFSIDGVDIKTVVIVGRISRMENRITQVDF 110

Query: 82  TVDDGTGTIGCKRWVNDTFDTNQMQEIQDGMYVRVNGHLKMFQSNKQIFAFSVRPVTNFD 141
            VDDGTG + C RW +   +T +M+ ++ GMYVR++GHLK+FQ  + +  FSVRPVT+F+
Sbjct: 111 VVDDGTGWVDCVRWCHARQETEEMEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPVTDFN 170

Query: 142 EITFHFIECIHDHLRNSKLQN---LKGNGSTQLQTSDSIVKTPVQNGSNGYHTTSSAIPS 201
           EI  HF EC++ H+ N+KL+     +   + + Q   S + TP +    G    S+  P+
Sbjct: 171 EIVHHFTECMYVHMYNTKLRGGSITQDTATPRPQMPYSTMPTPAKPYQTG---PSNQFPN 230

Query: 202 EQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKIPMEKILDSIRSLEDEGLI 261
           + +  D      + VL+YL  P  +  E G+H D ++++L+IP+ ++ +++  L ++G I
Sbjct: 231 QFN--DSMHGVKQTVLNYLNQPMHIVSEAGVHCDIIARELRIPLLQVKEALEQLSNDGCI 290

Query: 262 YSTIDEFHFKSAA 272
           YST+DE  FKS A
Sbjct: 291 YSTLDETCFKSTA 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZQ192.4e-6548.40Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ... [more]
Q6K9U21.1e-4640.77Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8LFJ81.6e-4539.11Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ... [more]
Q6H7J51.1e-4135.21Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q5Z8L11.0e-2340.63Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LEG76.3e-14698.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881710 PE=3 SV=1[more]
A0A1S3CR641.5e-14297.05replication protein A 32 kDa subunit A-like OS=Cucumis melo OX=3656 GN=LOC103503... [more]
A0A6J1F8S82.8e-13389.67replication protein A 32 kDa subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IMU11.3e-13088.28replication protein A 32 kDa subunit A-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1CUB84.6e-12084.87replication protein A 32 kDa subunit A OS=Momordica charantia OX=3673 GN=LOC1110... [more]
Match NameE-valueIdentityDescription
XP_004136317.13.12e-18898.89replication protein A 32 kDa subunit A [Cucumis sativus] >KGN60158.1 hypothetica... [more]
XP_008466326.18.23e-18497.05PREDICTED: replication protein A 32 kDa subunit A-like [Cucumis melo][more]
XP_038897330.13.44e-17692.99replication protein A 32 kDa subunit A-like [Benincasa hispida][more]
XP_022936562.11.28e-17189.67replication protein A 32 kDa subunit A-like [Cucurbita moschata][more]
KAG7024504.18.64e-17088.93Replication protein A 32 kDa subunit A [Cucurbita argyrosperma subsp. argyrosper... [more]
Match NameE-valueIdentityDescription
AT2G24490.11.7e-6648.40replicon protein A2 [more]
AT2G24490.21.7e-6648.40replicon protein A2 [more]
AT3G02920.11.1e-4639.11Replication protein A, subunit RPA32 [more]
AT3G02920.21.9e-4639.53Replication protein A, subunit RPA32 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 63..135
e-value: 1.3E-9
score: 37.9
IPR014646Replication factor A protein 2PIRSFPIRSF036949RPA32coord: 1..271
e-value: 4.6E-72
score: 240.3
NoneNo IPR availableGENE3D2.40.50.140coord: 29..160
e-value: 8.9E-43
score: 147.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR13989:SF34REPLICATION PROTEIN A 32 KDA SUBUNIT Acoord: 1..270
NoneNo IPR availableCDDcd04478RPA2_DBD_Dcoord: 62..155
e-value: 1.7236E-41
score: 136.19
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 203..271
e-value: 4.0E-16
score: 60.5
IPR014892Replication protein A, C-terminalPFAMPF08784RPA_Ccoord: 154..264
e-value: 7.8E-14
score: 52.2
IPR040260Replication factor A protein-likePANTHERPTHR13989REPLICATION PROTEIN A-RELATEDcoord: 1..270
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 32..158
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 207..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G070280.1Chy4G070280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006260 DNA replication
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0006289 nucleotide-excision repair
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
cellular_component GO:0000781 chromosome, telomeric region
cellular_component GO:0005662 DNA replication factor A complex
cellular_component GO:0035861 site of double-strand break
cellular_component GO:0005634 nucleus
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003676 nucleic acid binding