Chy4G069520 (gene) Cucumber (hystrix) v1

BLAST of Chy4G069520 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 523/711 (73.56%), Postives = 595/711 (83.68%), Query Frame = 0

Query: 65  TVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVI 124
           T+     R  +RV+E+EA    +T  + K+  G+  PLGVS+VD GINFA+FSQ+ATSV 
Sbjct: 57  TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116

Query: 125 ICLSL-----DGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHH 184
           +CLSL     D   DDGM+E  LDP  N+TGD WHIC++DL   NVLYGYR+DGP  W  
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQ 176

Query: 185 GHRYDAGTVLLDPYAKFVEGRRYFG-GENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKD 244
           GHR+D   +LLDPYAK V+G   FG    K + F GTYDF+S PFDWG+DYK PNIPEKD
Sbjct: 177 GHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKD 236

Query: 245 LVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQR 304
           LVIYEMNVR FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLP+FEFDELE QR
Sbjct: 237 LVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQR 296

Query: 305 HPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVY 364
             NPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFK+MVKALH+AGIEVILDVVY
Sbjct: 297 RSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVY 356

Query: 365 NHTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL 424
           NHTNEADD  PY TSFRGIDNKVYYMLD   N Q  NFSGCGNTLNCNHPVVMELIL+SL
Sbjct: 357 NHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSL 416

Query: 425 RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLY 484
           RHWV EYHVDGFRFDLASVLCR TDG+PLSAPPLIRAI+KD++LSRCKIIAEPWDCGGLY
Sbjct: 417 RHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLY 476

Query: 485 LVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNF 544
           LVG+FPNWDRWAEWNG+YRDD+R+FIKGD GMKGSFATRV+GSSDLY+VN+RKPYHGVNF
Sbjct: 477 LVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNF 536

Query: 545 VIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKN 604
           VIAHDGFTLRDLVSYN KHN+ANGEGGNDGCNDN SWNCGFEGET D  IK+LRTRQMKN
Sbjct: 537 VIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKN 596

Query: 605 FHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVI 664
           FHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHD +LN+F W++L+A+K++HFRFFSEVI
Sbjct: 597 FHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVI 656

Query: 665 KFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDD-NGEDVYLAFNAHEYFV 724
           KFR  H H+   ENFL + +ITWHE NWDN ESKFLA+TLHD   G D+Y+AFNAH+YFV
Sbjct: 657 KFRHSH-HVLKHENFLTQGEITWHEDNWDNSESKFLAFTLHDGIGGRDIYVAFNAHDYFV 716

Query: 725 NVSLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKA 769
              +P PP  ++WFRV DTNLESP DFV +G+ GV  +YNVAP+SSILL++
Sbjct: 717 KALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVADTYNVAPFSSILLQS 763

BLAST of Chy4G069520 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 520/719 (72.32%), Postives = 595/719 (82.75%), Query Frame = 0

Query: 57  RQGGVKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIF 116
           R   V+  T  A +      + E+      DT    K   G+AFPLGVS+V+ G+NFA+F
Sbjct: 63  RFDSVRSTTARAQNGNAGRSMTEERGCTMSDTEMPFKYSSGKAFPLGVSQVEGGLNFALF 122

Query: 117 SQHATSVIICLSLDGRIDD-----GMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRI 176
           SQHA+SVI+CL L GR  +      ++EF LD   N+TGDIWH+ ++ L    VLYGYR+
Sbjct: 123 SQHASSVILCLKLPGRGTEDEKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRV 182

Query: 177 DGPQGWHHGHRYDAGTVLLDPYAKFVEGRRYFG-GENKSSGFLGTYDFDSLPFDWGNDYK 236
            GPQGW  GHR+D+ TVLLDPYAK V GR+YFG  E KSS   GTYDFDS PFDWG+DY+
Sbjct: 183 GGPQGWDQGHRFDSSTVLLDPYAKLVSGRKYFGVAEEKSSQHFGTYDFDSSPFDWGDDYR 242

Query: 237 LPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFE 296
           LPN+PE DLVIYEMNVR FTADESSGL S++RGSYLG+I+KIPHLLELGVNAVELLP+FE
Sbjct: 243 LPNLPEADLVIYEMNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFE 302

Query: 297 FDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGI 356
           +DELEF+R+PNPRDHM+NTWGYSTINFFAPMSRYASAGGGP+ AS+E KQMVK LH AGI
Sbjct: 303 YDELEFKRYPNPRDHMVNTWGYSTINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGI 362

Query: 357 EVILDVVYNHTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVV 416
           EVILDVVYNHTNEADD  PY+TSFRGIDNKVYYMLDL  N +  NFSGCGNTLNCNHPVV
Sbjct: 363 EVILDVVYNHTNEADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVV 422

Query: 417 MELILESLRHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAE 476
            ELIL+SLRHWV EYH+DGFRFDLASVLCRG DG PL APPLI+ I+KDA+LSRCKIIAE
Sbjct: 423 KELILDSLRHWVEEYHIDGFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAE 482

Query: 477 PWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKR 536
           PWDCGGLYLVGRFPNWDRWAEWNG YRDD+R+FIKGD GMKG FATRV+GS+DLY+VN+R
Sbjct: 483 PWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNER 542

Query: 537 KPYHGVNFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKA 596
           KPYHGVNFVIAHDGFTL DLVSYN+KHNDANGEGG DGCNDNFSWNCG EGET D ++ +
Sbjct: 543 KPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLS 602

Query: 597 LRTRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDH 656
           LR+RQMKNFH+ALM SQGTPMMLMGDEYGHTRYGNNNSYGHD  +N+F WEQLE R+  H
Sbjct: 603 LRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGH 662

Query: 657 FRFFSEVIKFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDN-GEDVYLA 716
           FRFFSE+IKFR  +P +  R+ FLNKND+TWHE  W+N ESKFLA+T+HD N G D+YLA
Sbjct: 663 FRFFSEMIKFRHSNP-ILRRDRFLNKNDVTWHEDCWENQESKFLAFTVHDHNSGGDIYLA 722

Query: 717 FNAHEYFVNVSLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKA 769
           FNAH+YFV+  +P PP  + W RVVDTNLESP+D V +G+P  G  Y +APYSSILLKA
Sbjct: 723 FNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVPFTGPKYRIAPYSSILLKA 780

BLAST of Chy4G069520 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 6.8e-174
Identity = 344/767 (44.85%), Postives = 450/767 (58.67%), Query Frame = 0

Query: 57  RQGGVKLWTVSALS---SRDQDRVLEQE--------APENLDTGPMLKIFPGQAFPLGVS 116
           R+ G++    SA+      D++  +E+E         PE    G   ++  G   PLG +
Sbjct: 48  RRVGLRRSVASAVEVGVGEDEEEGVEEEEEEVEAVVMPERYALGGACRVLAGMPAPLGAT 107

Query: 117 EVDNGINFAIFSQHATSVIICLSL--DGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRK 176
            +D G+NFA++S  A++  +CL    D   D+   E  LDP  NRTG++WH+ I+     
Sbjct: 108 ALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVWHVFIEG-ELH 167

Query: 177 NVLYGYRIDGPQGWHHGHRYDAGTVLLDPYAKFVEGRRYFG----GENKSSGFLGTYDFD 236
           N+LYGYR DG    H G  +D   V++DPYAK V  R  +G    G +      G     
Sbjct: 168 NMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWPQMAGMIPLP 227

Query: 237 SLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELG 296
              FDW  D  L   P+KDLVIYEM++RGFT   SS +     G+Y+G I K+ +L ELG
Sbjct: 228 YSTFDWQGDLPL-RYPQKDLVIYEMHLRGFTKHSSSNVEHP--GTYIGAISKLDYLKELG 287

Query: 297 VNAVELLPLFEFDELEFQRHPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASR--- 356
           VN VEL+P  EF+ELE+    +     +N WGYSTINFF+PM RY+S  GG  N  R   
Sbjct: 288 VNCVELMPCHEFNELEYFSCSS----KMNFWGYSTINFFSPMIRYSS--GGIRNCGRDAI 347

Query: 357 -EFKQMVKALHAAGIEVILDVVYNHTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFN 416
            EFK  V+  H  GIEVI+DVV+NHT E ++  P I SFRGIDN  YYML  K  G+++N
Sbjct: 348 NEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGP-ILSFRGIDNSTYYMLAPK--GEFYN 407

Query: 417 FSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRG-------------- 476
           +SGCGNT NCNHPVV E I++ LR+WV E HVDGFRFDLAS++ RG              
Sbjct: 408 YSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPV 467

Query: 477 -----TDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIY 536
                T G PL+ PPLI  IS D IL   K+IAE WD GGLY VG+FP+W  W+EWNG Y
Sbjct: 468 EGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLYQVGQFPHWKIWSEWNGKY 527

Query: 537 RDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVIAHDGFTLRDLVSYNVK 596
           RD +R+FIKG  G  G FA  + GS  LY+   RKP+H +NFV AHDGFTL DLV+YN K
Sbjct: 528 RDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKK 587

Query: 597 HNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFHLALMTSQGTPMMLMGD 656
           +N +NGE   DG N N SWNCG EGE    S+K LR RQM+NF ++LM SQG PM  MGD
Sbjct: 588 YNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGD 647

Query: 657 EYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNK 716
           EYGHT+ GNNN+Y HD+ +N+F W++ E    D  RF S + KFR++   L    +F   
Sbjct: 648 EYGHTKGGNNNTYCHDHYVNYFRWDKKE-ESSDLQRFCSLMTKFRKQCESL-GLADFPTA 707

Query: 717 NDITWHESNWDNPE----SKFLAYTLHDDNGEDVYLAFNAHEYFVNVSLPSPPTKRRWFR 768
             + WH      P+    S+F+A++  D+   ++Y+AFNA      V LP  P   RW  
Sbjct: 708 QRLHWHGHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERP-GYRWEP 767

BLAST of Chy4G069520 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 1.5e-173
Identity = 338/718 (47.08%), Postives = 438/718 (61.00%), Query Frame = 0

Query: 94  IFPGQAFPLGVSEVDNGINFAIFSQHATSVIICL-SL-DGRIDDGMLEFKLDPDDNRTGD 153
           I  G   P G +  D+G+NF+++S ++ S  ICL SL D R +    E +LDP  NRTG 
Sbjct: 69  ISDGLPSPFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGH 128

Query: 154 IWHICIQDLRRKNVLYGYRIDGPQGWHHGHRYDAGTVLLDPYAKFVEGRRYFG----GEN 213
           +WH+ ++    K++LYGYR DG      GH YD+  +LLDPYAK +  R  FG     +N
Sbjct: 129 VWHVFLRG-DFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDN 188

Query: 214 KSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLG 273
                          FDW  D  L  +P+KDLVIYEM+VRGFT  ESS +     G+Y G
Sbjct: 189 CWPQMACMVPTREEEFDWEGDMHL-KLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQG 248

Query: 274 VIEKIPHLLELGVNAVELLPLFEFDELEFQRHPN-PRDHMINTWGYSTINFFAPMSRYAS 333
           V EK+ HL ELG+N +EL+P  EF+ELE+  +     DH +N WGYSTI FF+PM RYAS
Sbjct: 249 VAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYAS 308

Query: 334 AGGGPL--NASREFKQMVKALHAAGIEVILDVVYNHTNEADDVIPYITSFRGIDNKVYYM 393
           A        A  EFK +VK  H  GIEVI+DVV NHT E ++  P I SFRG+DN VYYM
Sbjct: 309 ASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGP-IFSFRGVDNSVYYM 368

Query: 394 LDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRG--- 453
           L  K  G+++N+SGCGNT NCNHPVV + IL+ LR+WV E HVDGFRFDL S++ R    
Sbjct: 369 LAPK--GEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSL 428

Query: 454 ----------------TDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPN 513
                           T G P+S PP+I  IS D IL   K+IAE WD GGLY VG FP+
Sbjct: 429 WDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPH 488

Query: 514 WDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVIAHDGF 573
           W  W+EWNG +RD +R+FIKG  G  G+FA  + GS +LY+   RKP+H +NF+ AHDGF
Sbjct: 489 WGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGF 548

Query: 574 TLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFHLALMT 633
           TL DLV+YN K+N ANGE  NDG N N+SWNCG EG+    S+K LR RQM+NF ++LM 
Sbjct: 549 TLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMV 608

Query: 634 SQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHP 693
           SQG PM+ MGDEYGHT+ GNNN+Y HDN +N+F W++ E    D FRF   +IKFR +  
Sbjct: 609 SQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECE 668

Query: 694 HLFSRENFLNKNDITWHE-----SNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 753
            L    +F     + WH       NW +  S+F+A++L D   +++Y+AFN       VS
Sbjct: 669 SL-GLNDFPTAKRLQWHGLAPEIPNW-SETSRFVAFSLVDSVKKEIYVAFNTSHLATLVS 728

Query: 754 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSS------------YNVAPYSSILL 767
           LP+ P   RW   VDT+  SP+D +   +P   ++            Y +  YSSI+L
Sbjct: 729 LPNRP-GYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIIL 775

BLAST of Chy4G069520 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 2.7e-130
Identity = 300/708 (42.37%), Postives = 389/708 (54.94%), Query Frame = 0

Query: 82  APENLDTGPML-KIFPGQAFPLGVSEVDNGINFAIFSQHATSVIICLSLDGRIDDGMLEF 141
           A E+  TGP L  ++PG A+PLG +    G NF++FS+ A  V +CL     ID+  +E 
Sbjct: 6   AGESDGTGPALPTVWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCL-----IDEDGVES 65

Query: 142 KLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHGHRYDAGTVLLDPYAKFVEGR 201
           ++ P D   G +WH  + ++      YG+R+ GP     GHR D   +LLDPY K   G 
Sbjct: 66  RI-PLDEVDGYVWHAYLPNI-TPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGD 125

Query: 202 RYFGGENKSSGFLGTYDFDSLP------------------FDWGNDYKLPNIPEKDLVIY 261
             F G+   S  +   D DS P                  FDW  D + P  P  + VIY
Sbjct: 126 FTF-GQALYSYDVNAVDPDSTPPMVDSLGHTMTSVVINPFFDWAYD-RSPRTPYHETVIY 185

Query: 262 EMNVRGFTADESSGLPSSTRGSYLGVIEK--IPHLLELGVNAVELLPLFEFDELEFQRHP 321
           E +V+G T    S +P   RG+Y G+     I HL EL V AVEL+P+ +F  L   R  
Sbjct: 186 EAHVKGMTQTHPS-IPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQF--LHDSRLL 245

Query: 322 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 381
           +    + N WGY+T  FFAP  +YAS      +A  EFK MV++LH AGIEVILDVVYNH
Sbjct: 246 DL--GLRNYWGYNTFGFFAPHHQYASTRQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNH 305

Query: 382 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 441
           T E + + P I +FRGIDN  YY L   +   Y +F+G GN+LN  HP  ++LI++SLR+
Sbjct: 306 TAEGNHLGPTI-NFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRY 365

Query: 442 WVVEYHVDGFRFDLASVLCRGT-DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCG-GLY 501
           WV+E HVDGFRFDLAS L R   D   LSA      + +D ++S+ K+IAEPWD G G Y
Sbjct: 366 WVIEMHVDGFRFDLASTLARELHDVDRLSA--FFDLVQQDPVVSQVKLIAEPWDVGEGGY 425

Query: 502 LVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNF 561
            VG FP    W EWNG YRD +R + +G+    G FA+R+ GSSDLY+   R+P   +NF
Sbjct: 426 QVGNFPG--LWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINF 485

Query: 562 VIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKN 621
           V AHDGFTL DLVSYN KHN+ANGE   DG + N SWNCG EG T+D  I ALR RQM+N
Sbjct: 486 VTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRN 545

Query: 622 FHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVI 681
               LM SQGTPM+  GDE G T+YGNNN Y  D+ L+   W  ++ +  D   F  +  
Sbjct: 546 MWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDWSLVD-KNADLLAFARKAT 605

Query: 682 KFRRKHPHLFSRENFL---------NKNDITW---------HES---NWDNPESKFL--- 738
             R+ H  +F R  F             DI W         HE     +D   + FL   
Sbjct: 606 TLRKNH-KVFRRRRFFEGEPIRSGDEVRDIAWLTPSGREMTHEDWGRGFDRCVAVFLNGE 665

BLAST of Chy4G069520 vs. ExPASy TrEMBL
Match: A0A0A0LE02 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889910 PE=4 SV=1)

HSP 1 Score: 1613.2 bits (4176), Expect = 0.0e+00
Identity = 763/775 (98.45%), Postives = 770/775 (99.35%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRSLPLHHYSTTLHFPL PSSCTGATHSLHFRLKRQVKLV GSSSSDSREIFSET+QGG
Sbjct: 1   MIRSLPLHHYSTTLHFPLLPSSCTGATHSLHFRLKRQVKLVPGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           TSV +CLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG
Sbjct: 121 TSVTLCLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HRYDAGTVLLDPYAKFVEGRRYFGGENKS+GFLGTYDFDSLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSTGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS EFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASLEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH
Sbjct: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKP HG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPCHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS
Sbjct: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLENELL 776
           LPSPPTKR+WFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLEN+LL
Sbjct: 721 LPSPPTKRKWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLENDLL 775

BLAST of Chy4G069520 vs. ExPASy TrEMBL
Match: A0A1S3CS22 (isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 1578.9 bits (4087), Expect = 0.0e+00
Identity = 747/770 (97.01%), Postives = 758/770 (98.44%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRSLPLHH STTL FPLFPSSCTGATH LHFRLKRQVKLVSGSSSSDSREIFSET+QGG
Sbjct: 1   MIRSLPLHH-STTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           TSV ICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDL+RKNVLYGYRIDGPQGWHHG
Sbjct: 121 TSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HRYD GT+LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH
Sbjct: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKPYHG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALR+RQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH+YFVNVS
Sbjct: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANL 771
           LPSPPT RRWFRVVDTNLESPHDFVLDGIPGVGSSYN+APYSSILLKANL
Sbjct: 721 LPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 769

BLAST of Chy4G069520 vs. ExPASy TrEMBL
Match: A0A5D3E5B0 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 PE=4 SV=1)

HSP 1 Score: 1578.9 bits (4087), Expect = 0.0e+00
Identity = 747/770 (97.01%), Postives = 758/770 (98.44%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRSLPLHH STTL FPLFPSSCTGATH LHFRLKRQVKLVSGSSSSDSREIFSET+QGG
Sbjct: 1   MIRSLPLHH-STTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           TSV ICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDL+RKNVLYGYRIDGPQGWHHG
Sbjct: 121 TSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HRYD GT+LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH
Sbjct: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKPYHG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALR+RQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH+YFVNVS
Sbjct: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANL 771
           LPSPPT RRWFRVVDTNLESPHDFVLDGIPGVGSSYN+APYSSILLKANL
Sbjct: 721 LPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 769

BLAST of Chy4G069520 vs. ExPASy TrEMBL
Match: A0A5A7TAT5 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 PE=4 SV=1)

HSP 1 Score: 1574.3 bits (4075), Expect = 0.0e+00
Identity = 747/771 (96.89%), Postives = 758/771 (98.31%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRSLPLHH STTL FPLFPSSCTGATH LHFRLKRQVKLVSGSSSSDSREIFSET+QGG
Sbjct: 1   MIRSLPLHH-STTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICI-QDLRRKNVLYGYRIDGPQGWHH 180
           TSV ICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICI QDL+RKNVLYGYRIDGPQGWHH
Sbjct: 121 TSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQQDLQRKNVLYGYRIDGPQGWHH 180

Query: 181 GHRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDL 240
           GHRYD GT+LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWGNDYKLPNIPEKDL
Sbjct: 181 GHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDL 240

Query: 241 VIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRH 300
           VIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRH
Sbjct: 241 VIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRH 300

Query: 301 PNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYN 360
           PNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKQMVKALHAAGIEVILDVVYN
Sbjct: 301 PNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYN 360

Query: 361 HTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLR 420
           HTNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLR
Sbjct: 361 HTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLR 420

Query: 421 HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYL 480
           HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYL
Sbjct: 421 HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYL 480

Query: 481 VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFV 540
           VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKPYHG+NFV
Sbjct: 481 VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFV 540

Query: 541 IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNF 600
           IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALR+RQMKNF
Sbjct: 541 IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNF 600

Query: 601 HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIK 660
           HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIK
Sbjct: 601 HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIK 660

Query: 661 FRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNV 720
           FRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH+YFVNV
Sbjct: 661 FRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNV 720

Query: 721 SLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANL 771
           SLPSPPT RRWFRVVDTNLESPHDFVLDGIPGVGSSYN+APYSSILLKANL
Sbjct: 721 SLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 770

BLAST of Chy4G069520 vs. ExPASy TrEMBL
Match: A0A6J1F9D1 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443282 PE=4 SV=1)

HSP 1 Score: 1476.8 bits (3822), Expect = 0.0e+00
Identity = 698/775 (90.06%), Postives = 728/775 (93.94%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRS+PLHH S  L  PLF SSCTGA HS+ FRLK Q K   GSSSS S+E+FSET+QGG
Sbjct: 1   MIRSIPLHH-SAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VK+ TVSA   RDQDRVLEQEAPENLDTGPMLK+ PGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           T+V ICLS + RIDD  LEFKLDPDDNRTGDIWHICIQDL RKNVLYGYRIDGPQGWHHG
Sbjct: 121 TAVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HR+D   VLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWG+DYKLPNI EKDLV
Sbjct: 181 HRFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVR FTADESSGL SS RGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPR+HMINTWGYSTINFFAPMSRYASAGGGPLNASREFK+MVKALH AGIEVILDVVYNH
Sbjct: 301 NPRNHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNEADDV PY TSFRGIDNKVYYMLDL+NNGQ+ NFSGCGNTLNCNHPVVMELIL+SLRH
Sbjct: 361 TNEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVVEYHVDGFRFDLASVLCRG DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVEYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRV+GSSDLYKVN RKPYHG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHN+ANGEGGNDGCNDNFSWNCGFEGETED +I+ALR+RQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHD ALNHFLW QLEARKRDHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFL +NDITWHESNWDNPESKFLAYTLHD NGEDVYLAFNAH+YFV+VS
Sbjct: 661 RRKHPHLFSRENFLGENDITWHESNWDNPESKFLAYTLHDGNGEDVYLAFNAHDYFVSVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLENELL 776
           LPSPPT R+WFRVVDTNLESP DFVLDGI G+GS+YN+APYSSILLKA LENELL
Sbjct: 721 LPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 774

BLAST of Chy4G069520 vs. NCBI nr
Match: XP_011652565.1 (isoamylase 3, chloroplastic isoform X1 [Cucumis sativus] >KGN60235.1 hypothetical protein Csa_002537 [Cucumis sativus])

HSP 1 Score: 1609 bits (4166), Expect = 0.0
Identity = 763/775 (98.45%), Postives = 770/775 (99.35%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRSLPLHHYSTTLHFPL PSSCTGATHSLHFRLKRQVKLV GSSSSDSREIFSET+QGG
Sbjct: 1   MIRSLPLHHYSTTLHFPLLPSSCTGATHSLHFRLKRQVKLVPGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           TSV +CLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG
Sbjct: 121 TSVTLCLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HRYDAGTVLLDPYAKFVEGRRYFGGENKS+GFLGTYDFDSLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSTGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS EFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASLEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH
Sbjct: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKP HG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPCHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS
Sbjct: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLENELL 775
           LPSPPTKR+WFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLEN+LL
Sbjct: 721 LPSPPTKRKWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLENDLL 775

BLAST of Chy4G069520 vs. NCBI nr
Match: XP_008466194.1 (PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >XP_008466195.1 PREDICTED: isoamylase 3, chloroplastic isoform X1 [Cucumis melo] >TYK31297.1 isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1574 bits (4075), Expect = 0.0
Identity = 747/770 (97.01%), Postives = 758/770 (98.44%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRSLPLHH STTL FPLFPSSCTGATH LHFRLKRQVKLVSGSSSSDSREIFSET+QGG
Sbjct: 1   MIRSLPLHH-STTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           TSV ICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDL+RKNVLYGYRIDGPQGWHHG
Sbjct: 121 TSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HRYD GT+LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWGNDYKLPNIPEKDLV
Sbjct: 181 HRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKQMVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH
Sbjct: 361 TNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKPYHG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALR+RQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH+YFVNVS
Sbjct: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANL 770
           LPSPPT RRWFRVVDTNLESPHDFVLDGIPGVGSSYN+APYSSILLKANL
Sbjct: 721 LPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 769

BLAST of Chy4G069520 vs. NCBI nr
Match: KAA0038687.1 (isoamylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1569 bits (4063), Expect = 0.0
Identity = 747/771 (96.89%), Postives = 758/771 (98.31%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRSLPLHH STTL FPLFPSSCTGATH LHFRLKRQVKLVSGSSSSDSREIFSET+QGG
Sbjct: 1   MIRSLPLHH-STTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKLWTVSA S RDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQ-DLRRKNVLYGYRIDGPQGWHH 180
           TSV ICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQ DL+RKNVLYGYRIDGPQGWHH
Sbjct: 121 TSVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQQDLQRKNVLYGYRIDGPQGWHH 180

Query: 181 GHRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDL 240
           GHRYD GT+LLDPYAKFVEGRR+FGGENKSSGFLGTYDF+SLPFDWGNDYKLPNIPEKDL
Sbjct: 181 GHRYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDL 240

Query: 241 VIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRH 300
           VIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRH
Sbjct: 241 VIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRH 300

Query: 301 PNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYN 360
           PNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKQMVKALHAAGIEVILDVVYN
Sbjct: 301 PNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYN 360

Query: 361 HTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLR 420
           HTNE DDV PY TSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLR
Sbjct: 361 HTNEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLR 420

Query: 421 HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYL 480
           HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYL
Sbjct: 421 HWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYL 480

Query: 481 VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFV 540
           VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLY VNKRKPYHG+NFV
Sbjct: 481 VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFV 540

Query: 541 IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNF 600
           IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALR+RQMKNF
Sbjct: 541 IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNF 600

Query: 601 HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIK 660
           HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIK
Sbjct: 601 HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIK 660

Query: 661 FRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNV 720
           FRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH+YFVNV
Sbjct: 661 FRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNV 720

Query: 721 SLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANL 770
           SLPSPPT RRWFRVVDTNLESPHDFVLDGIPGVGSSYN+APYSSILLKANL
Sbjct: 721 SLPSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 770

BLAST of Chy4G069520 vs. NCBI nr
Match: XP_038896292.1 (isoamylase 3, chloroplastic isoform X1 [Benincasa hispida] >XP_038896293.1 isoamylase 3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1478 bits (3827), Expect = 0.0
Identity = 707/774 (91.34%), Postives = 732/774 (94.57%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           M+R LPLHH S +L  PLF SSCT A +S+ FR K Q KL  GSSS  S++IFSE +QGG
Sbjct: 1   MVRYLPLHH-SASLQLPLFASSCTRAAYSVEFRSKPQGKLAPGSSSC-SQDIFSEIKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VKL TV A   RDQ+RVLEQEAPENLDTGPMLKIFPGQAFPLGVSEV +GINFAIFSQHA
Sbjct: 61  VKLCTVCAFGRRDQERVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVADGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           TSV ICLSL+ RIDD MLEFKLDPD+NRTGDIWHIC QDL RK+VLYGYRIDGPQGWHHG
Sbjct: 121 TSVTICLSLNERIDDRMLEFKLDPDNNRTGDIWHICFQDLPRKSVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HR+D  TVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNI EKDLV
Sbjct: 181 HRFDGSTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNILEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVRGFTADESSGL SSTRGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRGFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPRDHMIN WGYSTINFFAPMSRYASAGGGPLNASREFK+MVKALHAAGIEVILDVVYNH
Sbjct: 301 NPRDHMINMWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNEADDV PY TSFRGIDNKVYYMLDL+NNGQY NFSGCGNTLNCNHPVVMELIL+SLRH
Sbjct: 361 TNEADDVNPYTTSFRGIDNKVYYMLDLRNNGQYLNFSGCGNTLNCNHPVVMELILDSLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVV+YHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVDYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHND NGEGGNDG NDNFSWNCGFEGETEDTSIKALRTRQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNDVNGEGGNDGSNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLW+QLEARK DHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWDQLEARKTDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFL+KNDI+WHESNWDNPESKFLAYTLHDDNGEDVYLAFNAH+Y VNVS
Sbjct: 661 RRKHPHLFSRENFLSKNDISWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYSVNVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLENEL 774
           LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGV S+YN+APYSSILLKA LENEL
Sbjct: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVESTYNLAPYSSILLKAKLENEL 772

BLAST of Chy4G069520 vs. NCBI nr
Match: XP_022936809.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1472 bits (3812), Expect = 0.0
Identity = 698/775 (90.06%), Postives = 728/775 (93.94%), Query Frame = 0

Query: 1   MIRSLPLHHYSTTLHFPLFPSSCTGATHSLHFRLKRQVKLVSGSSSSDSREIFSETRQGG 60
           MIRS+PLHH S  L  PLF SSCTGA HS+ FRLK Q K   GSSSS S+E+FSET+QGG
Sbjct: 1   MIRSIPLHH-SAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGG 60

Query: 61  VKLWTVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHA 120
           VK+ TVSA   RDQDRVLEQEAPENLDTGPMLK+ PGQAFPLGVSEVDNGINFAIFSQHA
Sbjct: 61  VKICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHA 120

Query: 121 TSVIICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHG 180
           T+V ICLS + RIDD  LEFKLDPDDNRTGDIWHICIQDL RKNVLYGYRIDGPQGWHHG
Sbjct: 121 TAVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHG 180

Query: 181 HRYDAGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLV 240
           HR+D   VLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWG+DYKLPNI EKDLV
Sbjct: 181 HRFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLV 240

Query: 241 IYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHP 300
           IYEMNVR FTADESSGL SS RGSYLGVIEKIPHLLELGVNAVELLP+FEFDELEFQRHP
Sbjct: 241 IYEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHP 300

Query: 301 NPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVYNH 360
           NPR+HMINTWGYSTINFFAPMSRYASAGGGPLNASREFK+MVKALH AGIEVILDVVYNH
Sbjct: 301 NPRNHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNH 360

Query: 361 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 420
           TNEADDV PY TSFRGIDNKVYYMLDL+NNGQ+ NFSGCGNTLNCNHPVVMELIL+SLRH
Sbjct: 361 TNEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRH 420

Query: 421 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480
           WVVEYHVDGFRFDLASVLCRG DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV
Sbjct: 421 WVVEYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLV 480

Query: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVI 540
           GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRV+GSSDLYKVN RKPYHG+NFVI
Sbjct: 481 GRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVI 540

Query: 541 AHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFH 600
           AHDGFTLRDLVSYNVKHN+ANGEGGNDGCNDNFSWNCGFEGETED +I+ALR+RQMKNFH
Sbjct: 541 AHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFH 600

Query: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKF 660
           LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHD ALNHFLW QLEARKRDHFRFFSEVIKF
Sbjct: 601 LALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKF 660

Query: 661 RRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 720
           RRKHPHLFSRENFL +NDITWHESNWDNPESKFLAYTLHD NGEDVYLAFNAH+YFV+VS
Sbjct: 661 RRKHPHLFSRENFLGENDITWHESNWDNPESKFLAYTLHDGNGEDVYLAFNAHDYFVSVS 720

Query: 721 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKANLENELL 775
           LPSPPT R+WFRVVDTNLESP DFVLDGI G+GS+YN+APYSSILLKA LENELL
Sbjct: 721 LPSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 774

BLAST of Chy4G069520 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 523/711 (73.56%), Postives = 595/711 (83.68%), Query Frame = 0

Query: 65  TVSALSSRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHATSVI 124
           T+     R  +RV+E+EA    +T  + K+  G+  PLGVS+VD GINFA+FSQ+ATSV 
Sbjct: 57  TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116

Query: 125 ICLSL-----DGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHH 184
           +CLSL     D   DDGM+E  LDP  N+TGD WHIC++DL   NVLYGYR+DGP  W  
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQQ 176

Query: 185 GHRYDAGTVLLDPYAKFVEGRRYFG-GENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKD 244
           GHR+D   +LLDPYAK V+G   FG    K + F GTYDF+S PFDWG+DYK PNIPEKD
Sbjct: 177 GHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKD 236

Query: 245 LVIYEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQR 304
           LVIYEMNVR FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLP+FEFDELE QR
Sbjct: 237 LVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQR 296

Query: 305 HPNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKQMVKALHAAGIEVILDVVY 364
             NPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFK+MVKALH+AGIEVILDVVY
Sbjct: 297 RSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVY 356

Query: 365 NHTNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESL 424
           NHTNEADD  PY TSFRGIDNKVYYMLD   N Q  NFSGCGNTLNCNHPVVMELIL+SL
Sbjct: 357 NHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSL 416

Query: 425 RHWVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLY 484
           RHWV EYHVDGFRFDLASVLCR TDG+PLSAPPLIRAI+KD++LSRCKIIAEPWDCGGLY
Sbjct: 417 RHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLY 476

Query: 485 LVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNF 544
           LVG+FPNWDRWAEWNG+YRDD+R+FIKGD GMKGSFATRV+GSSDLY+VN+RKPYHGVNF
Sbjct: 477 LVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNF 536

Query: 545 VIAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKN 604
           VIAHDGFTLRDLVSYN KHN+ANGEGGNDGCNDN SWNCGFEGET D  IK+LRTRQMKN
Sbjct: 537 VIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKN 596

Query: 605 FHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVI 664
           FHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHD +LN+F W++L+A+K++HFRFFSEVI
Sbjct: 597 FHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVI 656

Query: 665 KFRRKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDD-NGEDVYLAFNAHEYFV 724
           KFR  H H+   ENFL + +ITWHE NWDN ESKFLA+TLHD   G D+Y+AFNAH+YFV
Sbjct: 657 KFRHSH-HVLKHENFLTQGEITWHEDNWDNSESKFLAFTLHDGIGGRDIYVAFNAHDYFV 716

Query: 725 NVSLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSSYNVAPYSSILLKA 769
              +P PP  ++WFRV DTNLESP DFV +G+ GV  +YNVAP+SSILL++
Sbjct: 717 KALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVADTYNVAPFSSILLQS 763

BLAST of Chy4G069520 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 611.3 bits (1575), Expect = 1.1e-174
Identity = 338/718 (47.08%), Postives = 438/718 (61.00%), Query Frame = 0

Query: 94  IFPGQAFPLGVSEVDNGINFAIFSQHATSVIICL-SL-DGRIDDGMLEFKLDPDDNRTGD 153
           I  G   P G +  D+G+NF+++S ++ S  ICL SL D R +    E +LDP  NRTG 
Sbjct: 69  ISDGLPSPFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGH 128

Query: 154 IWHICIQDLRRKNVLYGYRIDGPQGWHHGHRYDAGTVLLDPYAKFVEGRRYFG----GEN 213
           +WH+ ++    K++LYGYR DG      GH YD+  +LLDPYAK +  R  FG     +N
Sbjct: 129 VWHVFLRG-DFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDN 188

Query: 214 KSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPSSTRGSYLG 273
                          FDW  D  L  +P+KDLVIYEM+VRGFT  ESS +     G+Y G
Sbjct: 189 CWPQMACMVPTREEEFDWEGDMHL-KLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQG 248

Query: 274 VIEKIPHLLELGVNAVELLPLFEFDELEFQRHPN-PRDHMINTWGYSTINFFAPMSRYAS 333
           V EK+ HL ELG+N +EL+P  EF+ELE+  +     DH +N WGYSTI FF+PM RYAS
Sbjct: 249 VAEKLDHLKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYAS 308

Query: 334 AGGGPL--NASREFKQMVKALHAAGIEVILDVVYNHTNEADDVIPYITSFRGIDNKVYYM 393
           A        A  EFK +VK  H  GIEVI+DVV NHT E ++  P I SFRG+DN VYYM
Sbjct: 309 ASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGP-IFSFRGVDNSVYYM 368

Query: 394 LDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRG--- 453
           L  K  G+++N+SGCGNT NCNHPVV + IL+ LR+WV E HVDGFRFDL S++ R    
Sbjct: 369 LAPK--GEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSL 428

Query: 454 ----------------TDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPN 513
                           T G P+S PP+I  IS D IL   K+IAE WD GGLY VG FP+
Sbjct: 429 WDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPH 488

Query: 514 WDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFVIAHDGF 573
           W  W+EWNG +RD +R+FIKG  G  G+FA  + GS +LY+   RKP+H +NF+ AHDGF
Sbjct: 489 WGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGF 548

Query: 574 TLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNFHLALMT 633
           TL DLV+YN K+N ANGE  NDG N N+SWNCG EG+    S+K LR RQM+NF ++LM 
Sbjct: 549 TLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMV 608

Query: 634 SQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHP 693
           SQG PM+ MGDEYGHT+ GNNN+Y HDN +N+F W++ E    D FRF   +IKFR +  
Sbjct: 609 SQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECE 668

Query: 694 HLFSRENFLNKNDITWHE-----SNWDNPESKFLAYTLHDDNGEDVYLAFNAHEYFVNVS 753
            L    +F     + WH       NW +  S+F+A++L D   +++Y+AFN       VS
Sbjct: 669 SL-GLNDFPTAKRLQWHGLAPEIPNW-SETSRFVAFSLVDSVKKEIYVAFNTSHLATLVS 728

Query: 754 LPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSS------------YNVAPYSSILL 767
           LP+ P   RW   VDT+  SP+D +   +P   ++            Y +  YSSI+L
Sbjct: 729 LPNRP-GYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIIL 775

BLAST of Chy4G069520 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 384.0 bits (985), Expect = 2.8e-106
Identity = 261/736 (35.46%), Postives = 372/736 (50.54%), Query Frame = 0

Query: 69  LSSRDQDRVLEQEAPENLDTGPMLKI--FPGQAFPLGVSE--VDNGINFAIFSQHATSVI 128
           +S RD D    QE   + DT   + +    G   PLG+S    D+  NF+ FS+ +T+V+
Sbjct: 203 VSGRDPD---GQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVV 262

Query: 129 ICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHGHRYD 188
           +CL  D   D   LE  LDP  NRTGD+WH  + D     V YGYR             +
Sbjct: 263 LCLYDDSTTDKPALELDLDPYVNRTGDVWHASV-DNTWDFVRYGYRCKETAHSKEDVDVE 322

Query: 189 AGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLVIYEM 248
              ++LDPYA  V       G++ S  +LG+    S  FDWG D   PNIP + L++Y +
Sbjct: 323 GEPIVLDPYATVV-------GKSVSQKYLGSLS-KSPSFDWGEDVS-PNIPLEKLLVYRL 382

Query: 249 NVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHPNPRD 308
           NV+GFT   SS LPS+  G++ GV EK+ HL  LG NAV L P+F F E   Q+ P    
Sbjct: 383 NVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSE---QKGP---- 442

Query: 309 HMINTWGYSTINFFAPMSRYASAGGGPLN----ASREFKQMVKALHAAGIEVILDVVYNH 368
                  Y   +FF+PM  Y     GP N    A    K MVK LH+ GIEV+L+VV+ H
Sbjct: 443 -------YFPFHFFSPMDIY-----GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTH 502

Query: 369 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 428
           T ++        + RGID+  YY     N+    ++      LNCN+PVV +L+LESLR+
Sbjct: 503 TADSG-------ALRGIDDSSYYYKGRANDLDSKSY------LNCNYPVVQQLVLESLRY 562

Query: 429 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD-CGGLYL 488
           WV E+HVDGF F  AS L RG  G  LS PPL+ AI+ D +L+  K+IA+ WD    +  
Sbjct: 563 WVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPK 622

Query: 489 VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFV 548
             RFP+W RWAE N  Y  ++R F++G  G+    ATR+ GS D++  + R P    N++
Sbjct: 623 EVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVF-TDGRGPAFSFNYI 682

Query: 549 IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNF 608
             + G +L D+VS++                   SWNCG EG T  +++   R +Q++NF
Sbjct: 683 SRNSGLSLVDIVSFS-----------GPELASELSWNCGEEGATNKSAVLQRRLKQIRNF 742

Query: 609 HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQL-EARKRDHFRFFSEVI 668
                 S G P++ MGDE G +  G+        +   F W  L  A      +F S + 
Sbjct: 743 LFIQYISLGVPVLNMGDECGISTRGSPLL----ESRKPFDWNLLASAFGTQITQFISFMT 802

Query: 669 KFRRKHPHLFSRENFLNKNDITWHESN-----WDNPESKFLAYTLHDDNGE--------- 728
             R +   +F R +FL   +I W+ ++     W++P SKFLA  +  ++ E         
Sbjct: 803 SVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEP 862

Query: 729 ------DVYLAFNAHEYFVNVSLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSS--- 768
                 D+++ FNA ++  +V LPS P   +W R+VDT L  P  F ++G   V      
Sbjct: 863 NEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQ 876

BLAST of Chy4G069520 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 384.0 bits (985), Expect = 2.8e-106
Identity = 261/736 (35.46%), Postives = 372/736 (50.54%), Query Frame = 0

Query: 69  LSSRDQDRVLEQEAPENLDTGPMLKI--FPGQAFPLGVSE--VDNGINFAIFSQHATSVI 128
           +S RD D    QE   + DT   + +    G   PLG+S    D+  NF+ FS+ +T+V+
Sbjct: 203 VSGRDPD---GQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVV 262

Query: 129 ICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLRRKNVLYGYRIDGPQGWHHGHRYD 188
           +CL  D   D   LE  LDP  NRTGD+WH  + D     V YGYR             +
Sbjct: 263 LCLYDDSTTDKPALELDLDPYVNRTGDVWHASV-DNTWDFVRYGYRCKETAHSKEDVDVE 322

Query: 189 AGTVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNIPEKDLVIYEM 248
              ++LDPYA  V       G++ S  +LG+    S  FDWG D   PNIP + L++Y +
Sbjct: 323 GEPIVLDPYATVV-------GKSVSQKYLGSLS-KSPSFDWGEDVS-PNIPLEKLLVYRL 382

Query: 249 NVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPLFEFDELEFQRHPNPRD 308
           NV+GFT   SS LPS+  G++ GV EK+ HL  LG NAV L P+F F E   Q+ P    
Sbjct: 383 NVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFSE---QKGP---- 442

Query: 309 HMINTWGYSTINFFAPMSRYASAGGGPLN----ASREFKQMVKALHAAGIEVILDVVYNH 368
                  Y   +FF+PM  Y     GP N    A    K MVK LH+ GIEV+L+VV+ H
Sbjct: 443 -------YFPFHFFSPMDIY-----GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTH 502

Query: 369 TNEADDVIPYITSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRH 428
           T ++        + RGID+  YY     N+    ++      LNCN+PVV +L+LESLR+
Sbjct: 503 TADSG-------ALRGIDDSSYYYKGRANDLDSKSY------LNCNYPVVQQLVLESLRY 562

Query: 429 WVVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWD-CGGLYL 488
           WV E+HVDGF F  AS L RG  G  LS PPL+ AI+ D +L+  K+IA+ WD    +  
Sbjct: 563 WVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMMPK 622

Query: 489 VGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGVNFV 548
             RFP+W RWAE N  Y  ++R F++G  G+    ATR+ GS D++  + R P    N++
Sbjct: 623 EVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVF-TDGRGPAFSFNYI 682

Query: 549 IAHDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRTRQMKNF 608
             + G +L D+VS++                   SWNCG EG T  +++   R +Q++NF
Sbjct: 683 SRNSGLSLVDIVSFS-----------GPELASELSWNCGEEGATNKSAVLQRRLKQIRNF 742

Query: 609 HLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQL-EARKRDHFRFFSEVI 668
                 S G P++ MGDE G +  G+        +   F W  L  A      +F S + 
Sbjct: 743 LFIQYISLGVPVLNMGDECGISTRGSPLL----ESRKPFDWNLLASAFGTQITQFISFMT 802

Query: 669 KFRRKHPHLFSRENFLNKNDITWHESN-----WDNPESKFLAYTLHDDNGE--------- 728
             R +   +F R +FL   +I W+ ++     W++P SKFLA  +  ++ E         
Sbjct: 803 SVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEEEETASLAEP 862

Query: 729 ------DVYLAFNAHEYFVNVSLPSPPTKRRWFRVVDTNLESPHDFVLDGIPGVGSS--- 768
                 D+++ FNA ++  +V LPS P   +W R+VDT L  P  F ++G   V      
Sbjct: 863 NEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDTALPFPGFFSVEGETVVAEEPLQ 876

BLAST of Chy4G069520 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 115.9 bits (289), Expect = 1.4e-25
Identity = 98/337 (29.08%), Postives = 149/337 (44.21%), Query Frame = 0

Query: 191 DPYAK--FVEGRRYFGGENKSSGFLGTYDFDSL-PFDWGN--DYKLPNIPEKDLVIYEMN 250
           DPYA+    +GR+          FL   D D L P  W N  D K       D+ IYE++
Sbjct: 289 DPYARGLSADGRK---------TFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELH 348

Query: 251 VRGFTADESSGLPSSTRGSYLGVIEK-------IPHLLELGVNAVELLPLFEFDELEFQR 310
           VR F+A++ +  P + RG YL    K       +  L++ G+  + LLP F+F +++ ++
Sbjct: 349 VRDFSANDETVEPEN-RGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEK 408

Query: 311 --------------HPNPRDHMIN----------TWGYSTINFFAPMSRYASAGGGPLNA 370
                          P+  +               WGY+ + +  P   YAS   GP   
Sbjct: 409 ENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRI 468

Query: 371 SREFKQMVKALHAAGIEVILDVVYNHTNEA---------DDVIPYITSFRGIDNKVYYML 430
             EF++MV+AL+  G+ V+LDVVYNH + +         D ++P            YY+ 
Sbjct: 469 I-EFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVP-----------GYYL- 528

Query: 431 DLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLCRGTDGA 483
             +N+  +   S C N     H +V  LI + L +WVV Y VDGFRFDL   + + T   
Sbjct: 529 -RRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVN 588