Chy4G069460 (gene) Cucumber (hystrix) v1

Overview
NameChy4G069460
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionProtein kinase domain-containing protein
LocationchrH04: 2057147 .. 2059861 (-)
RNA-Seq ExpressionChy4G069460
SyntenyChy4G069460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAAGAAAAGGAAAGGAAGTGAGACTGATGGACCGTTAAATTTACAAGACGATGTGACAGAATCTCATGAATCGCGATCTTCTAAACTGAAGGCTCATTACTCGCTCGAAGATTATACGAGATTGAATAAAAGGTGCAAGGAAGATGTGGGTATTGAACCGGTTCTTTCTTGTAAGAGTAGATTTGCAGGCATAGCTACAGCTCCACCCAGTGGGAGTTCGTCTCTGATTCTTCCTGGTAGAGGTTTGAAGAGGAAGATCGGATGCATAGACGTGGCTACTCAAATCGGGCGGAAACACAACATTGAGAATGATTATGTTTCAGGTAAGACTATTGGGCATGGAAAATTTGGCTCAGTTTGGCTATGTAAATGTAAGGTTAGTGGAGCTGAGTATGCTTGCAAGACCTTGAAAAAAGGGGAAGAGACTGTACATAGGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCTGGTGTTGTGACATTGTTGGCAGTGTACGAGGACTTCGAATGTTTTCACCTGGTTATGGAACTGTGTGGAGGAGGCCGTTTGGTCGAACAGATGGGCAGTGAAGGCCAATATTCAGAGCATCGAGCAGCCAATATACTCAAGGAAGTGATGTTGGTTATAAAGTATTGTCATGATATGGGGGTTGTACATAGAGACATAAAGCCTGAAAATATTCTTCTCACAACATCTGGAAAGATAAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATATGGTATGCATTCTTTAATCTGTAATAGAGAGTACATCTTTGTATGTTTCTCTTCAAGTATTAGTTGCCTGATCTTCTTTGTAGTCCTCAATTGCAGACATCCAATGTTTATTGATCACCAGCTATGGAAGTGTTCTCATTTTTTTGTTTCTTTTGTTACCTTTGCTTGTATCTTTCTGTGACCGTGCAAATGTCTTATAAAGAAATAAGTGAGGAATGTCACTACAACTTAGTAGTATTTGAAGCACTGTAAATACAGCACTACAAAGTTATTAGATGAAATATGCTCAAGAAACAAAGAAAGAAAATCTACGATCAAACTAAAGAAGTTGCTTAAAATTCCAATTAGCAAAAATAGATGTTGAACTATAGTCATAAAAAAAAAAGTTGTTAATTTTACTCCTTGCGTAATCCAATCCTGTTAGTCTATATGGTTCCTTGGATAACCATTGTTCAGATTCAAGGTATGCTTATGCATATAAGAATGATTGGACCGTAGCTCACATTTGGTGTTTAGCGACAATGCTTTAGCTTTGGAGTGAGAGGATGCTTGTAAGGAGAGAAATTACTTTGGGAGTAAATTTTTTTTTGAGGGCCAAATGCTATGCTTGTAGAGAGATCAGATGTTGAGAGGGTGGGCTAAGCTTTATGAGAAGAACATCCATTTCAAATTGGACCCCTATGCTTTCACAACTTTGATGTATTGTTATTAAGATCACGTTTCCAAACGCTGGCCTAAACAGATTATTGTTTCCAACAATATATTCCAAAAACAAGTTTGTCAAGTGAGTTTATTGTATTTAGGAGCATTTTTAGAAAACATAAAATGTAAACTGATTAGGTACCACAATCCTGTGAGGTAAGACTTAATTGGATGGACAATGATATCGTAAATTGCAGCATTACATAAATTTTACATCATAATAGTTTTCTATTATATATTATGTTTCTTGCTTCTACTTTCTTGTAATGATGCCGCTTGTGTATTTCTTATTGTTGCTATCAACGGTGTGCAAATTTTGATGTTCTTTTACATTTAGAATAAGAGTTCCAAACATCTACTTATTTAAGTTATTCGATTGCGTTCTGCAGGCCAGAGTTTAACTGGTTTGGCTGGAAGCCCAGCTTATGTAGCCCCTGAAGTGTTGACAGGAAAGTACTCTGAGAAGGTGGATATTTGGAGTGCTGGTGTGCTCCTCCATGCTCTTTTGGTTGGCACTCTTCCTTTCCAAGGTGACTCATTAGAATCAGTTTTCGAAGCAATAAAGAACTCTAAGCTCGACTTCCACTCAGGGATGTGGGAGTCCATATCTAAACCTGCCCGCGATCTCATTGGGAGAATGCTGACAAGAGATATTTCAGCTAGGATAACAGCAGAAGAAGTCTTACGTAAGTTTGTTTTACTTATTGGTCTCTTACCCTGATGATTAAGTAATACATAATCAGTTTTATTTGCTATCAATATTTTGAAATTCAGAAATAAGCATAAACTATTGCTATGTTTATATGTTATAAAGTCCAATATCCTTAAATAGATTGATGCCAATCTTAATTTCTTAGTGTAATCTGATTTGGATCCAAATGGTGTTATTTCAGGACATCCCTGGATATTGTTTTATACCGAACGAACGTTGAAGGCTCTCCCGATCAAACTAAAATTGAAGAATCAAGAATCTTCCCAACAGTCACCCATTGCAGCCAAAATTAAGTCTGACAGAAACAGGATTGATAGTGCAGCTAATATGACCTCCCTTAACAAAGTCTCTAACCTTTCCTCATCCGAGAGTTGCAATGCTGATGGTGATGATGTCGACGATTGCTTTCTGGTGGATGCACTTGCTACAGCTATTTCACATGTGAGAATTTCCGAGCCAAAGAGAAGTAGACTATGTGTTCCTACAGGACCTATCGAGCAGCACAGTTCCTCTAACATGAAAGCTAACAATCTTTGTAAAGCGTTTTGA

mRNA sequence

ATGAGGAAGAAAAGGAAAGGAAGTGAGACTGATGGACCGTTAAATTTACAAGACGATGTGACAGAATCTCATGAATCGCGATCTTCTAAACTGAAGGCTCATTACTCGCTCGAAGATTATACGAGATTGAATAAAAGGTGCAAGGAAGATGTGGGTATTGAACCGGTTCTTTCTTGTAAGAGTAGATTTGCAGGCATAGCTACAGCTCCACCCAGTGGGAGTTCGTCTCTGATTCTTCCTGGTAGAGGTTTGAAGAGGAAGATCGGATGCATAGACGTGGCTACTCAAATCGGGCGGAAACACAACATTGAGAATGATTATGTTTCAGGTAAGACTATTGGGCATGGAAAATTTGGCTCAGTTTGGCTATGTAAATGTAAGGTTAGTGGAGCTGAGTATGCTTGCAAGACCTTGAAAAAAGGGGAAGAGACTGTACATAGGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCTGGTGTTGTGACATTGTTGGCAGTGTACGAGGACTTCGAATGTTTTCACCTGGTTATGGAACTGTGTGGAGGAGGCCGTTTGGTCGAACAGATGGGCAGTGAAGGCCAATATTCAGAGCATCGAGCAGCCAATATACTCAAGGAAGTGATGTTGGTTATAAAGTATTGTCATGATATGGGGGTTGTACATAGAGACATAAAGCCTGAAAATATTCTTCTCACAACATCTGGAAAGATAAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATATGGCCAGAGTTTAACTGGTTTGGCTGGAAGCCCAGCTTATGTAGCCCCTGAAGTGTTGACAGGAAAGTACTCTGAGAAGGTGGATATTTGGAGTGCTGGTGTGCTCCTCCATGCTCTTTTGGTTGGCACTCTTCCTTTCCAAGGTGACTCATTAGAATCAGTTTTCGAAGCAATAAAGAACTCTAAGCTCGACTTCCACTCAGGGATGTGGGAGTCCATATCTAAACCTGCCCGCGATCTCATTGGGAGAATGCTGACAAGAGATATTTCAGCTAGGATAACAGCAGAAGAAGTCTTACGACATCCCTGGATATTGTTTTATACCGAACGAACGTTGAAGGCTCTCCCGATCAAACTAAAATTGAAGAATCAAGAATCTTCCCAACAGTCACCCATTGCAGCCAAAATTAAGTCTGACAGAAACAGGATTGATAGTGCAGCTAATATGACCTCCCTTAACAAAGTCTCTAACCTTTCCTCATCCGAGAGTTGCAATGCTGATGGTGATGATGTCGACGATTGCTTTCTGGTGGATGCACTTGCTACAGCTATTTCACATGTGAGAATTTCCGAGCCAAAGAGAAGTAGACTATGTGTTCCTACAGGACCTATCGAGCAGCACAGTTCCTCTAACATGAAAGCTAACAATCTTTGTAAAGCGTTTTGA

Coding sequence (CDS)

ATGAGGAAGAAAAGGAAAGGAAGTGAGACTGATGGACCGTTAAATTTACAAGACGATGTGACAGAATCTCATGAATCGCGATCTTCTAAACTGAAGGCTCATTACTCGCTCGAAGATTATACGAGATTGAATAAAAGGTGCAAGGAAGATGTGGGTATTGAACCGGTTCTTTCTTGTAAGAGTAGATTTGCAGGCATAGCTACAGCTCCACCCAGTGGGAGTTCGTCTCTGATTCTTCCTGGTAGAGGTTTGAAGAGGAAGATCGGATGCATAGACGTGGCTACTCAAATCGGGCGGAAACACAACATTGAGAATGATTATGTTTCAGGTAAGACTATTGGGCATGGAAAATTTGGCTCAGTTTGGCTATGTAAATGTAAGGTTAGTGGAGCTGAGTATGCTTGCAAGACCTTGAAAAAAGGGGAAGAGACTGTACATAGGGAGGTGGAGATAATGCAGCACTTATCTGGCTACCCTGGTGTTGTGACATTGTTGGCAGTGTACGAGGACTTCGAATGTTTTCACCTGGTTATGGAACTGTGTGGAGGAGGCCGTTTGGTCGAACAGATGGGCAGTGAAGGCCAATATTCAGAGCATCGAGCAGCCAATATACTCAAGGAAGTGATGTTGGTTATAAAGTATTGTCATGATATGGGGGTTGTACATAGAGACATAAAGCCTGAAAATATTCTTCTCACAACATCTGGAAAGATAAAGCTTGCAGATTTTGGCCTGGCCACTAGGATTTCATATGGCCAGAGTTTAACTGGTTTGGCTGGAAGCCCAGCTTATGTAGCCCCTGAAGTGTTGACAGGAAAGTACTCTGAGAAGGTGGATATTTGGAGTGCTGGTGTGCTCCTCCATGCTCTTTTGGTTGGCACTCTTCCTTTCCAAGGTGACTCATTAGAATCAGTTTTCGAAGCAATAAAGAACTCTAAGCTCGACTTCCACTCAGGGATGTGGGAGTCCATATCTAAACCTGCCCGCGATCTCATTGGGAGAATGCTGACAAGAGATATTTCAGCTAGGATAACAGCAGAAGAAGTCTTACGACATCCCTGGATATTGTTTTATACCGAACGAACGTTGAAGGCTCTCCCGATCAAACTAAAATTGAAGAATCAAGAATCTTCCCAACAGTCACCCATTGCAGCCAAAATTAAGTCTGACAGAAACAGGATTGATAGTGCAGCTAATATGACCTCCCTTAACAAAGTCTCTAACCTTTCCTCATCCGAGAGTTGCAATGCTGATGGTGATGATGTCGACGATTGCTTTCTGGTGGATGCACTTGCTACAGCTATTTCACATGTGAGAATTTCCGAGCCAAAGAGAAGTAGACTATGTGTTCCTACAGGACCTATCGAGCAGCACAGTTCCTCTAACATGAAAGCTAACAATCTTTGTAAAGCGTTTTGA

Protein sequence

MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCKSRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKLADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTERTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGDDVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF*
Homology
BLAST of Chy4G069460 vs. ExPASy Swiss-Prot
Match: Q8W490 (Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2 PE=2 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.1e-170
Identity = 311/479 (64.93%), Postives = 378/479 (78.91%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSET+G  NL DD++ S  SRSS  ++H+SLE Y RL KRCKE   +E V S K
Sbjct: 1   MRKKRKGSETEGWENLPDDLSCSTASRSSNFRSHFSLEGYARLKKRCKETEAVESVGSFK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
            R AG+ATAPP G+SSL+  GRGLKRKIGCIDV+TQ GRK+ I++DYV G+ IG GKFGS
Sbjct: 61  RRIAGVATAPPCGASSLVSSGRGLKRKIGCIDVSTQTGRKNKIDDDYVFGRNIGKGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           V +CK + +G E+ACKTLKKGEETVHREVEIMQHLSG+P VVTL AVYE+ +CFHLVMEL
Sbjct: 121 VRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVVTLHAVYEESDCFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRL++QM   G+YSE RAANI K++MLVI YCH+MGVVHRDIKPENILLT +GKI+L
Sbjct: 181 CSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKIQL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLA RI+ GQ+L+GLAGSPAYVAPEVL+  YSEKVD+WSAGVLL+ALL G LPF+GD
Sbjct: 241 ADFGLAMRIAKGQTLSGLAGSPAYVAPEVLSENYSEKVDVWSAGVLLYALLSGVLPFKGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SL+++FEAIKN KLDF++G+WES+SKPARDL+ RMLTR+ SARITA+EVLRHPWILFYT+
Sbjct: 301 SLDAIFEAIKNVKLDFNTGVWESVSKPARDLLARMLTREESARITADEVLRHPWILFYTD 360

Query: 361 RTLKALPIKLKLKNQESS-----QQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESC 420
           RTLK + IK K K+Q  S      +SP     K+D NR D    + S +   + S++E  
Sbjct: 361 RTLKTMCIKSKHKSQAGSSTCLQNRSPTE---KTDLNRADREKKIPSDSPTDSFSNTE-- 420

Query: 421 NADGDDVDDCFLVDALATAISHVRISEPKRSRLCVPT-GPIEQHSSSNM-KANNLCKAF 473
               ++ D+  +VD L  AI++VRISEPKRSR+C PT  PIEQ  SSN+   + LC+AF
Sbjct: 421 ----EEEDESGVVDVLVVAIANVRISEPKRSRVCSPTNNPIEQQHSSNLTSTSTLCRAF 470

BLAST of Chy4G069460 vs. ExPASy Swiss-Prot
Match: Q6F3A6 (Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK10 PE=2 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 7.5e-67
Identity = 125/292 (42.81%), Postives = 184/292 (63.01%), Query Frame = 0

Query: 74  SSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEY 133
           SS+ +L G  LKRK              ++++ Y  G+ +G G+FG+ +LC  + +G E+
Sbjct: 112 SSAGLLVGSVLKRK------------TESLKDKYSLGRKLGQGQFGTTYLCVERATGKEF 171

Query: 134 ACKTLKKGE-------ETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMELCGGGRL 193
           ACK++ K +       E V RE++IM HL+G+P V+++   YED    HLVMELC GG L
Sbjct: 172 ACKSILKRKLVTDDDVEDVRREIQIMYHLAGHPNVISIRGAYEDAVAVHLVMELCAGGEL 231

Query: 194 VEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILL---TTSGKIKLADF 253
            +++  +G Y+E +AA + + ++ V++ CH MGV+HRD+KPEN L    T    +K  DF
Sbjct: 232 FDRIVQKGHYTERKAAELARVIVGVVEVCHSMGVMHRDLKPENFLFADQTEEAALKTIDF 291

Query: 254 GLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLE 313
           GL+     GQ  T + GSP YVAPEVL  KY ++ D+WSAGV+++ LL G  PF  ++ +
Sbjct: 292 GLSIFFRPGQVFTDVVGSPYYVAPEVLKKKYGQEADVWSAGVIIYILLCGVPPFWAENEQ 351

Query: 314 SVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWI 356
            +FE + + +LDF S  W SIS+ A+DL+ RML RD   R+TA EVLRHPW+
Sbjct: 352 GIFEEVLHGRLDFQSEPWPSISEGAKDLVRRMLVRDPKKRLTAHEVLRHPWV 391

BLAST of Chy4G069460 vs. ExPASy Swiss-Prot
Match: P53684 (Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK7 PE=2 SV=2)

HSP 1 Score: 248.1 bits (632), Expect = 2.1e-64
Identity = 121/270 (44.81%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 97  IGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTL-------KKGEETVHRE 156
           +GRK  ++   Y+ G+ +G G+FG+ +LC    +G EYACKT+       K+  E V RE
Sbjct: 68  LGRKTADLREHYIIGRKLGQGQFGTTYLCTEINTGCEYACKTIPKRKLITKEDVEDVRRE 127

Query: 157 VEIMQHLSGYPGVVTLLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEV 216
           ++IM HLSG+  VV +  VYED +  H+VMELC GG L +++  +G YSE +AA +++ +
Sbjct: 128 IQIMHHLSGHKNVVAIKDVYEDGQAVHIVMELCAGGELFDRIQEKGHYSERKAAELIRII 187

Query: 217 MLVIKYCHDMGVVHRDIKPENILLTTSG---KIKLADFGLATRISYGQSLTGLAGSPAYV 276
           + ++  CH +GV+HRD+KPEN LL        IK  DFGL+     GQ  T L GSP YV
Sbjct: 188 VSIVAMCHSLGVMHRDLKPENFLLLDKDDDLSIKAIDFGLSVFFKPGQVFTELVGSPYYV 247

Query: 277 APEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESIS 336
           APEVL  +Y  + D+WSAGV+L+ LL G  PF  ++ + +F+A+    +DF S  W  IS
Sbjct: 248 APEVLHKRYGPESDVWSAGVILYVLLSGVPPFWAETQQGIFDAVLKGHIDFQSDPWPKIS 307

Query: 337 KPARDLIGRMLTRDISARITAEEVLRHPWI 356
             A+DLI +ML+   S R+ A EVLRHPWI
Sbjct: 308 DSAKDLIRKMLSHCPSERLKAHEVLRHPWI 337

BLAST of Chy4G069460 vs. ExPASy Swiss-Prot
Match: Q9ZV15 (Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.0e-64
Identity = 117/275 (42.55%), Postives = 176/275 (64.00%), Query Frame = 0

Query: 91  IDVATQIGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGE------- 150
           + + + +GRK  N+++ Y  G+ +G G+FG+ +LC  K +G E+ACKT+ K +       
Sbjct: 117 LQIDSVLGRKTENLKDIYSVGRKLGQGQFGTTFLCVDKKTGKEFACKTIAKRKLTTPEDV 176

Query: 151 ETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAA 210
           E V RE++IM HLSG+P V+ ++  YED    H+VME+C GG L +++   G Y+E +AA
Sbjct: 177 EDVRREIQIMHHLSGHPNVIQIVGAYEDAVAVHVVMEICAGGELFDRIIQRGHYTEKKAA 236

Query: 211 NILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISYGQSLTGLA 270
            + + ++ VI+ CH +GV+HRD+KPEN L  +  +   +K  DFGL+     G++ T + 
Sbjct: 237 ELARIIVGVIEACHSLGVMHRDLKPENFLFVSGDEEAALKTIDFGLSVFFKPGETFTDVV 296

Query: 271 GSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSG 330
           GSP YVAPEVL   YS + D+WSAGV+++ LL G  PF  ++ + +FE +    LDF S 
Sbjct: 297 GSPYYVAPEVLRKHYSHECDVWSAGVIIYILLSGVPPFWDETEQGIFEQVLKGDLDFISE 356

Query: 331 MWESISKPARDLIGRMLTRDISARITAEEVLRHPW 355
            W S+S+ A+DL+ RML RD   R+T  EVL HPW
Sbjct: 357 PWPSVSESAKDLVRRMLIRDPKKRMTTHEVLCHPW 391

BLAST of Chy4G069460 vs. ExPASy Swiss-Prot
Match: Q9ZSA2 (Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.0e-64
Identity = 122/275 (44.36%), Postives = 178/275 (64.73%), Query Frame = 0

Query: 92  DVATQIGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTL-------KKGEE 151
           D  T +G+   +I   Y  GK +G G+FG  ++CK   +G  YACK++       K+ +E
Sbjct: 64  DPDTILGKPFEDIRKFYSLGKELGRGQFGITYMCKEIGTGNTYACKSILKRKLISKQDKE 123

Query: 152 TVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAAN 211
            V RE++IMQ+LSG P +V +   YED +  HLVMELC GG L +++ ++G YSE  AA 
Sbjct: 124 DVKREIQIMQYLSGQPNIVEIKGAYEDRQSIHLVMELCAGGELFDRIIAQGHYSERAAAG 183

Query: 212 ILKEVMLVIKYCHDMGVVHRDIKPENILLTT---SGKIKLADFGLATRISYGQSLTGLAG 271
           I++ ++ V++ CH MGVVHRD+KPEN LL++   +  +K  DFGL+  I  G+    + G
Sbjct: 184 IIRSIVNVVQICHFMGVVHRDLKPENFLLSSKEENAMLKATDFGLSVFIEEGKVYRDIVG 243

Query: 272 SPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGM 331
           S  YVAPEVL   Y +++DIWSAGV+L+ LL G  PF  ++ + +F+ +   ++DF S  
Sbjct: 244 SAYYVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKGEIDFVSEP 303

Query: 332 WESISKPARDLIGRMLTRDISARITAEEVLRHPWI 356
           W SIS+ A+DL+ +MLT+D   RITA +VL HPWI
Sbjct: 304 WPSISESAKDLVRKMLTKDPKRRITAAQVLEHPWI 338

BLAST of Chy4G069460 vs. ExPASy TrEMBL
Match: A0A0A0LJM7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889970 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.3e-268
Identity = 469/472 (99.36%), Postives = 471/472 (99.79%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNLQD+VTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYED ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLN+VSNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 473
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. ExPASy TrEMBL
Match: A0A5D3E5W2 (Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005900 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.7e-263
Identity = 458/472 (97.03%), Postives = 468/472 (99.15%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQD+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIA+KIKSDRNRIDSAAN+TSLN++SNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 473
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. ExPASy TrEMBL
Match: A0A1S3CQM3 (serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.7e-263
Identity = 458/472 (97.03%), Postives = 468/472 (99.15%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQD+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIA+KIKSDRNRIDSAAN+TSLN++SNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 473
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. ExPASy TrEMBL
Match: A0A6J1IGJ4 (serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC111476741 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.4e-254
Identity = 444/472 (94.07%), Postives = 460/472 (97.46%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+D+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES Q SPI+AK KSDRNRIDSAAN+TSLN+VS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQPSPISAKTKSDRNRIDSAANLTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 473
           D +DCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDEDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. ExPASy TrEMBL
Match: A0A6J1F9Y1 (serine/threonine-protein kinase PEPKR2 OS=Cucurbita moschata OX=3662 GN=LOC111443442 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.4e-254
Identity = 443/472 (93.86%), Postives = 459/472 (97.25%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+D+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIE+DYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIEHDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTT+GKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTAGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES Q SPI+AK KSDRNRIDSAAN TSLN+VS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQPSPISAKTKSDRNRIDSAANSTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 473
           D DDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. NCBI nr
Match: XP_031738766.1 (serine/threonine-protein kinase PEPKR2 [Cucumis sativus] >KGN60241.1 hypothetical protein Csa_002436 [Cucumis sativus])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 469/472 (99.36%), Postives = 471/472 (99.79%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNLQD+VTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPLNLQDNVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYED ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDSECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLN+VSNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNEVSNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. NCBI nr
Match: XP_008466187.1 (PREDICTED: serine/threonine-protein kinase PEPKR2 [Cucumis melo] >KAA0038682.1 serine/threonine-protein kinase PEPKR2 [Cucumis melo var. makuwa] >TYK31292.1 serine/threonine-protein kinase PEPKR2 [Cucumis melo var. makuwa])

HSP 1 Score: 918 bits (2372), Expect = 0.0
Identity = 458/472 (97.03%), Postives = 468/472 (99.15%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NLQD+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLQDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKY+EKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYTEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLKALPIKLKLKNQESSQQSPIA+KIKSDRNRIDSAAN+TSLN++SNLSSSESCNADGD
Sbjct: 361 RTLKALPIKLKLKNQESSQQSPIASKIKSDRNRIDSAANVTSLNEISNLSSSESCNADGD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. NCBI nr
Match: XP_038899232.1 (serine/threonine-protein kinase PEPKR2 [Benincasa hispida] >XP_038899233.1 serine/threonine-protein kinase PEPKR2 [Benincasa hispida])

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 448/472 (94.92%), Postives = 458/472 (97.03%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGP NL D+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK
Sbjct: 1   MRKKRKGSETDGPSNLPDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCID+ATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDMATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCK SG EYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKASGTEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEEAECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSLTGLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES  QSPIAAKIK DR RIDSAAN+TSLN+VSNLSSSESCNAD D
Sbjct: 361 RTLKTLPIKLKLKNQESCLQSPIAAKIKCDRIRIDSAANVTSLNEVSNLSSSESCNADVD 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           DVDDCFLVDALATAISHVRISEPKRSR+CVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DVDDCFLVDALATAISHVRISEPKRSRVCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. NCBI nr
Match: XP_023535980.1 (serine/threonine-protein kinase PEPKR2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 446/472 (94.49%), Postives = 460/472 (97.46%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+D+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES QQSPI+AK KSDRNRIDSAAN TSLN+VS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQQSPISAKTKSDRNRIDSAANSTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           D DDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. NCBI nr
Match: XP_022976306.1 (serine/threonine-protein kinase PEPKR2 [Cucurbita maxima])

HSP 1 Score: 888 bits (2295), Expect = 0.0
Identity = 444/472 (94.07%), Postives = 460/472 (97.46%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSETDGPLNL+D+VTESH+SRSSKLKAHYSLEDYTRLNKRCKEDVGIEPV SCK
Sbjct: 1   MRKKRKGSETDGPLNLRDNVTESHDSRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVPSCK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
           SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSG+TIGHGKFGS
Sbjct: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGETIGHGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           VWLCKCKVSGAEYACKTLKK EETVHREVEIMQHLSGYPGVVTLLAVYE+ ECFHLVMEL
Sbjct: 121 VWLCKCKVSGAEYACKTLKKAEETVHREVEIMQHLSGYPGVVTLLAVYEESECFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRLV+QM SEG+YSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL
Sbjct: 181 CRGGRLVDQMVSEGRYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLATRISYGQSL GLAGSPAYVAPEVL+GKYSEKVDIWSAGVLLHALLVG LPFQGD
Sbjct: 241 ADFGLATRISYGQSLIGLAGSPAYVAPEVLSGKYSEKVDIWSAGVLLHALLVGALPFQGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDIS+RITAEEVLRHPWILFYTE
Sbjct: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISSRITAEEVLRHPWILFYTE 360

Query: 361 RTLKALPIKLKLKNQESSQQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESCNADGD 420
           RTLK LPIKLKLKNQES Q SPI+AK KSDRNRIDSAAN+TSLN+VS+LSSSESCNA+G+
Sbjct: 361 RTLKTLPIKLKLKNQESCQPSPISAKTKSDRNRIDSAANLTSLNEVSDLSSSESCNANGE 420

Query: 421 DVDDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472
           D +DCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF
Sbjct: 421 DDEDCFLVDALATAISHVRISEPKRSRLCVPTGPIEQHSSSNMKANNLCKAF 472

BLAST of Chy4G069460 vs. TAIR 10
Match: AT1G12680.1 (phosphoenolpyruvate carboxylase-related kinase 2 )

HSP 1 Score: 600.1 bits (1546), Expect = 1.5e-171
Identity = 311/479 (64.93%), Postives = 378/479 (78.91%), Query Frame = 0

Query: 1   MRKKRKGSETDGPLNLQDDVTESHESRSSKLKAHYSLEDYTRLNKRCKEDVGIEPVLSCK 60
           MRKKRKGSET+G  NL DD++ S  SRSS  ++H+SLE Y RL KRCKE   +E V S K
Sbjct: 1   MRKKRKGSETEGWENLPDDLSCSTASRSSNFRSHFSLEGYARLKKRCKETEAVESVGSFK 60

Query: 61  SRFAGIATAPPSGSSSLILPGRGLKRKIGCIDVATQIGRKHNIENDYVSGKTIGHGKFGS 120
            R AG+ATAPP G+SSL+  GRGLKRKIGCIDV+TQ GRK+ I++DYV G+ IG GKFGS
Sbjct: 61  RRIAGVATAPPCGASSLVSSGRGLKRKIGCIDVSTQTGRKNKIDDDYVFGRNIGKGKFGS 120

Query: 121 VWLCKCKVSGAEYACKTLKKGEETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMEL 180
           V +CK + +G E+ACKTLKKGEETVHREVEIMQHLSG+P VVTL AVYE+ +CFHLVMEL
Sbjct: 121 VRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVVTLHAVYEESDCFHLVMEL 180

Query: 181 CGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGKIKL 240
           C GGRL++QM   G+YSE RAANI K++MLVI YCH+MGVVHRDIKPENILLT +GKI+L
Sbjct: 181 CSGGRLIDQMVKVGRYSEQRAANIFKDLMLVINYCHEMGVVHRDIKPENILLTAAGKIQL 240

Query: 241 ADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGD 300
           ADFGLA RI+ GQ+L+GLAGSPAYVAPEVL+  YSEKVD+WSAGVLL+ALL G LPF+GD
Sbjct: 241 ADFGLAMRIAKGQTLSGLAGSPAYVAPEVLSENYSEKVDVWSAGVLLYALLSGVLPFKGD 300

Query: 301 SLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDISARITAEEVLRHPWILFYTE 360
           SL+++FEAIKN KLDF++G+WES+SKPARDL+ RMLTR+ SARITA+EVLRHPWILFYT+
Sbjct: 301 SLDAIFEAIKNVKLDFNTGVWESVSKPARDLLARMLTREESARITADEVLRHPWILFYTD 360

Query: 361 RTLKALPIKLKLKNQESS-----QQSPIAAKIKSDRNRIDSAANMTSLNKVSNLSSSESC 420
           RTLK + IK K K+Q  S      +SP     K+D NR D    + S +   + S++E  
Sbjct: 361 RTLKTMCIKSKHKSQAGSSTCLQNRSPTE---KTDLNRADREKKIPSDSPTDSFSNTE-- 420

Query: 421 NADGDDVDDCFLVDALATAISHVRISEPKRSRLCVPT-GPIEQHSSSNM-KANNLCKAF 473
               ++ D+  +VD L  AI++VRISEPKRSR+C PT  PIEQ  SSN+   + LC+AF
Sbjct: 421 ----EEEDESGVVDVLVVAIANVRISEPKRSRVCSPTNNPIEQQHSSNLTSTSTLCRAF 470

BLAST of Chy4G069460 vs. TAIR 10
Match: AT1G12580.1 (phosphoenolpyruvate carboxylase-related kinase 1 )

HSP 1 Score: 252.7 bits (644), Expect = 5.9e-67
Identity = 123/264 (46.59%), Postives = 176/264 (66.67%), Query Frame = 0

Query: 102 NIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGE-------ETVHREVEIMQH 161
           N+++ YV G+ +G G+FG + +C  K++G   ACK++ K         +++  E+ IM  
Sbjct: 39  NLKDRYVLGEQLGWGQFGVIRVCSDKLTGERLACKSISKDRLVTQDDMKSIKLEIAIMAK 98

Query: 162 LSGYPGVVTLLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKY 221
           L+G+P VV L AVYE+ +  HLVMELC GG L  ++   G+YSE RA  + K +M V+K+
Sbjct: 99  LAGHPNVVNLKAVYEEKDSVHLVMELCAGGELFHKLEKYGRYSEVRARVLFKHLMQVVKF 158

Query: 222 CHDMGVVHRDIKPENILLTT---SGKIKLADFGLATRISYGQSLTGLAGSPAYVAPEVLT 281
           CHD G+VHRD+KPENIL+ T   S  IKLADFGLAT I  G+ L+G  GSP Y+APEVL 
Sbjct: 159 CHDSGIVHRDLKPENILMATMSSSSPIKLADFGLATYIKPGEKLSGTVGSPFYIAPEVLA 218

Query: 282 GKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESISKPARDL 341
           G Y++  D+WSAGV+L+ LL G  PF G +   +F+A++ + L F +  W++I+  A+DL
Sbjct: 219 GGYNQAADVWSAGVILYILLSGAPPFWGKTKSKIFDAVRAADLRFSAEPWDNITSYAKDL 278

Query: 342 IGRMLTRDISARITAEEVLRHPWI 356
           I  ML  D S R++A+EVL H W+
Sbjct: 279 IRGMLCVDPSQRLSADEVLAHSWM 302

BLAST of Chy4G069460 vs. TAIR 10
Match: AT2G38910.1 (calcium-dependent protein kinase 20 )

HSP 1 Score: 246.5 bits (628), Expect = 4.2e-65
Identity = 117/275 (42.55%), Postives = 176/275 (64.00%), Query Frame = 0

Query: 91  IDVATQIGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTLKKGE------- 150
           + + + +GRK  N+++ Y  G+ +G G+FG+ +LC  K +G E+ACKT+ K +       
Sbjct: 117 LQIDSVLGRKTENLKDIYSVGRKLGQGQFGTTFLCVDKKTGKEFACKTIAKRKLTTPEDV 176

Query: 151 ETVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAA 210
           E V RE++IM HLSG+P V+ ++  YED    H+VME+C GG L +++   G Y+E +AA
Sbjct: 177 EDVRREIQIMHHLSGHPNVIQIVGAYEDAVAVHVVMEICAGGELFDRIIQRGHYTEKKAA 236

Query: 211 NILKEVMLVIKYCHDMGVVHRDIKPENILLTTSGK---IKLADFGLATRISYGQSLTGLA 270
            + + ++ VI+ CH +GV+HRD+KPEN L  +  +   +K  DFGL+     G++ T + 
Sbjct: 237 ELARIIVGVIEACHSLGVMHRDLKPENFLFVSGDEEAALKTIDFGLSVFFKPGETFTDVV 296

Query: 271 GSPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSG 330
           GSP YVAPEVL   YS + D+WSAGV+++ LL G  PF  ++ + +FE +    LDF S 
Sbjct: 297 GSPYYVAPEVLRKHYSHECDVWSAGVIIYILLSGVPPFWDETEQGIFEQVLKGDLDFISE 356

Query: 331 MWESISKPARDLIGRMLTRDISARITAEEVLRHPW 355
            W S+S+ A+DL+ RML RD   R+T  EVL HPW
Sbjct: 357 PWPSVSESAKDLVRRMLIRDPKKRMTTHEVLCHPW 391

BLAST of Chy4G069460 vs. TAIR 10
Match: AT4G04720.1 (calcium-dependent protein kinase 21 )

HSP 1 Score: 246.5 bits (628), Expect = 4.2e-65
Identity = 122/275 (44.36%), Postives = 178/275 (64.73%), Query Frame = 0

Query: 92  DVATQIGRK-HNIENDYVSGKTIGHGKFGSVWLCKCKVSGAEYACKTL-------KKGEE 151
           D  T +G+   +I   Y  GK +G G+FG  ++CK   +G  YACK++       K+ +E
Sbjct: 64  DPDTILGKPFEDIRKFYSLGKELGRGQFGITYMCKEIGTGNTYACKSILKRKLISKQDKE 123

Query: 152 TVHREVEIMQHLSGYPGVVTLLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAAN 211
            V RE++IMQ+LSG P +V +   YED +  HLVMELC GG L +++ ++G YSE  AA 
Sbjct: 124 DVKREIQIMQYLSGQPNIVEIKGAYEDRQSIHLVMELCAGGELFDRIIAQGHYSERAAAG 183

Query: 212 ILKEVMLVIKYCHDMGVVHRDIKPENILLTT---SGKIKLADFGLATRISYGQSLTGLAG 271
           I++ ++ V++ CH MGVVHRD+KPEN LL++   +  +K  DFGL+  I  G+    + G
Sbjct: 184 IIRSIVNVVQICHFMGVVHRDLKPENFLLSSKEENAMLKATDFGLSVFIEEGKVYRDIVG 243

Query: 272 SPAYVAPEVLTGKYSEKVDIWSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGM 331
           S  YVAPEVL   Y +++DIWSAGV+L+ LL G  PF  ++ + +F+ +   ++DF S  
Sbjct: 244 SAYYVAPEVLRRSYGKEIDIWSAGVILYILLSGVPPFWAENEKGIFDEVIKGEIDFVSEP 303

Query: 332 WESISKPARDLIGRMLTRDISARITAEEVLRHPWI 356
           W SIS+ A+DL+ +MLT+D   RITA +VL HPWI
Sbjct: 304 WPSISESAKDLVRKMLTKDPKRRITAAQVLEHPWI 338

BLAST of Chy4G069460 vs. TAIR 10
Match: AT1G76040.2 (calcium-dependent protein kinase 29 )

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-64
Identity = 121/255 (47.45%), Postives = 164/255 (64.31%), Query Frame = 0

Query: 111 KTIGHGKFGSVWLCKCKVSGAEYACKTLKKGE-------ETVHREVEIMQHLSGYPGVVT 170
           K +G G+FG  + C  K +G EYACK++ K +       E V REV I+QHL+G P +V 
Sbjct: 116 KELGRGQFGITYKCTDKSNGREYACKSISKRKLIRRKDIEDVRREVMILQHLTGQPNIVE 175

Query: 171 LLAVYEDFECFHLVMELCGGGRLVEQMGSEGQYSEHRAANILKEVMLVIKYCHDMGVVHR 230
               YED +  HLVMELC GG L +++  +G YSE  AANI ++++ V+  CH MGVVHR
Sbjct: 176 FRGAYEDKDNLHLVMELCSGGELFDRIIKKGSYSEKEAANIFRQIVNVVHVCHFMGVVHR 235

Query: 231 DIKPENILLTTS---GKIKLADFGLATRISYGQSLTGLAGSPAYVAPEVLTGKYSEKVDI 290
           D+KPEN LL ++     IK  DFGL+  I  G+    + GS  YVAPEVL   Y +++D+
Sbjct: 236 DLKPENFLLVSNEEDSPIKATDFGLSVFIEEGKVYRDIVGSAYYVAPEVLHRNYGKEIDV 295

Query: 291 WSAGVLLHALLVGTLPFQGDSLESVFEAIKNSKLDFHSGMWESISKPARDLIGRMLTRDI 350
           WSAGV+L+ LL G  PF G++ +++FEAI   KLD  +  W +IS+ A+DLI +ML RD 
Sbjct: 296 WSAGVMLYILLSGVPPFWGETEKTIFEAILEGKLDLETSPWPTISESAKDLIRKMLIRDP 355

Query: 351 SARITAEEVLRHPWI 356
             RITA E L HPW+
Sbjct: 356 KKRITAAEALEHPWM 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4902.1e-17064.93Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2... [more]
Q6F3A67.5e-6742.81Calcium-dependent protein kinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
P536842.1e-6444.81Calcium-dependent protein kinase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q9ZV156.0e-6442.55Calcium-dependent protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CPK20 PE=... [more]
Q9ZSA26.0e-6444.36Calcium-dependent protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CPK21 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LJM71.3e-26899.36Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G889... [more]
A0A5D3E5W21.7e-26397.03Serine/threonine-protein kinase PEPKR2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CQM31.7e-26397.03serine/threonine-protein kinase PEPKR2 OS=Cucumis melo OX=3656 GN=LOC103503677 P... [more]
A0A6J1IGJ41.4e-25494.07serine/threonine-protein kinase PEPKR2 OS=Cucurbita maxima OX=3661 GN=LOC1114767... [more]
A0A6J1F9Y19.4e-25493.86serine/threonine-protein kinase PEPKR2 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
XP_031738766.10.099.36serine/threonine-protein kinase PEPKR2 [Cucumis sativus] >KGN60241.1 hypothetica... [more]
XP_008466187.10.097.03PREDICTED: serine/threonine-protein kinase PEPKR2 [Cucumis melo] >KAA0038682.1 s... [more]
XP_038899232.10.094.92serine/threonine-protein kinase PEPKR2 [Benincasa hispida] >XP_038899233.1 serin... [more]
XP_023535980.10.094.49serine/threonine-protein kinase PEPKR2 [Cucurbita pepo subsp. pepo][more]
XP_022976306.10.094.07serine/threonine-protein kinase PEPKR2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G12680.11.5e-17164.93phosphoenolpyruvate carboxylase-related kinase 2 [more]
AT1G12580.15.9e-6746.59phosphoenolpyruvate carboxylase-related kinase 1 [more]
AT2G38910.14.2e-6542.55calcium-dependent protein kinase 20 [more]
AT4G04720.14.2e-6544.36calcium-dependent protein kinase 21 [more]
AT1G76040.21.2e-6447.45calcium-dependent protein kinase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 107..355
e-value: 1.1E-94
score: 330.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 107..355
e-value: 5.7E-68
score: 229.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 107..355
score: 48.823795
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 98..420
e-value: 8.3E-86
score: 290.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 24..464
NoneNo IPR availablePANTHERPTHR24349:SF261SERINE/THREONINE-PROTEIN KINASE PEPKR2coord: 24..464
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 106..354
e-value: 7.04442E-115
score: 336.371
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 113..136
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 220..232
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 101..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G069460.1Chy4G069460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity