Chy4G069330 (gene) Cucumber (hystrix) v1

BLAST of Chy4G069330 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 625.9 bits (1613), Expect = 4.3e-178
Identity = 313/530 (59.06%), Postives = 396/530 (74.72%), Query Frame = 0

Query: 40  ICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHL-QNSSTLPNSI 99
           IC+   +PSYC +VL  ++ G+IY  GR S+RRSLS+A RF+ +I+  L +       S 
Sbjct: 38  ICRFAPDPSYCRSVL-PNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKST 97

Query: 100 IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNT 159
           +GAL+DC++LA L M++L +S +  + T + L+ S+A+ + + LSA +TN  TCL+GL +
Sbjct: 98  VGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKS 157

Query: 160 VASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPG---KKHLQFRKGP 219
            AS + L  D    L + TK Y +SL LF+KGWVPRR R+R +  P    KK   FR G 
Sbjct: 158 TASENGLSGD----LFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGK 217

Query: 220 LPLRMSRHDRAVYNSVANRRKLLSSSDGVLVNGVVVVSQDGHGDFLNITDAINAAPNNSL 279
           LPL+M+   RAVYN+V  R+ L S +D V V+ +V V Q+G G+F  I  AI AAPN + 
Sbjct: 218 LPLKMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTD 277

Query: 280 ASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAV 339
            S+GYFLIY+ AG+Y+EYV VP  K+Y++MIGDGINQT+ITGNRSV DGWTTFNSATF +
Sbjct: 278 GSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFIL 337

Query: 340 AAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECD 399
           +   F+ VNITI+NTAG  KGQAVALRSG D+ VFYSCSFE YQDTLYTHSLRQF+RECD
Sbjct: 338 SGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECD 397

Query: 400 IYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPE 459
           +YGTVDFIFGNAAVV QNCN+YPR P  GQ+N +TAQGR+DPNQNTGT+IH C+IR   +
Sbjct: 398 VYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADD 457

Query: 460 LAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-LSTLYYGEYNDTG 519
           LA S +Y  KTYLGRPWK+YSRTV MQT+IDGF+ P GW+ W+G + LSTLYY EYN+TG
Sbjct: 458 LATS-NYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTG 517

Query: 520 GGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 565
            GSDT NRVTW GYHVI NATDA+NFT++NFLVG+ W+  T VP+ GG +
Sbjct: 518 PGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Chy4G069330 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 604.4 bits (1557), Expect = 1.3e-171
Identity = 311/574 (54.18%), Postives = 413/574 (71.95%), Query Frame = 0

Query: 10  LLASFLILLTLFTS---SFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSG 69
           +L+  L L+TLF S    F +  +L     +  ICK T +P +C +V  Q+  G++ + G
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYG 60

Query: 70  RFSIRRSLSKATRFLDLIEKHL-QNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNG 129
           RFS+R+SL+++ +F   I+++L +N++ L  S +GAL+DC+YLA L  ++L  SF  VN 
Sbjct: 61  RFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNI 120

Query: 130 T--DRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSL 189
           T   + L++SKAD IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+
Sbjct: 121 TTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSV 180

Query: 190 SLDLFTKGWVPRRNR---NRTLEHPGKKH------LQFRKGPLPLRMSRHDRAVYNSVAN 249
           SL LFTKGWVP++ +   + +  HP   H        FR G LPL+M+ H RAVY S++ 
Sbjct: 181 SLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSR 240

Query: 250 RRKLLSSSD--GVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQ 309
           R+     +D   VLV+ +V V+Q+G G+F  IT+A+N+APN +  + GYF+IY+ +GVY+
Sbjct: 241 RKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYE 300

Query: 310 EYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTA 369
           E V +   K+YL+MIGDGIN+T++TGNR+V DGWTTFNSATFAV +  F+AVN+T +NTA
Sbjct: 301 ENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTA 360

Query: 370 GAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVF 429
           G  K QAVA+RS AD+ +FYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVF
Sbjct: 361 GPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVF 420

Query: 430 QNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRP 489
           Q+CN+YPR P   Q N ITAQGR+DPNQNTG SIHNC+I+   +L  S++Y  KTYLGRP
Sbjct: 421 QDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDL-VSSNYTVKTYLGRP 480

Query: 490 WKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-LSTLYYGEYNDTGGGSDTKNRVTWAGYHV 549
           WK+YSRTV+MQ++ID  V P GW  W G + LSTLYY EYN+TG GS T +RV W GYHV
Sbjct: 481 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 540

Query: 550 INNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 565
           I N+TDA NFT+ NFL+GD W+  + VPY  G +
Sbjct: 541 I-NSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Chy4G069330 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 3.9e-171
Identity = 323/578 (55.88%), Postives = 410/578 (70.93%), Query Frame = 0

Query: 15  LILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSL 74
           LI L+ F  S  +  S  T   +  ICK T +P YC +V   S+ GN+   G FSIR+SL
Sbjct: 8   LITLSFFLQSVLA--SSQTLSNSSTICKTTPDPKYCKSVFPHSQ-GNVQQYGCFSIRKSL 67

Query: 75  SKATRFLDLIEKHLQNSSTLPN-SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRK---- 134
           S++ +F+  ++++++ ++ L   ++I AL+DC++LA L M++L  SF  VN T  K    
Sbjct: 68  SQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFK 127

Query: 135 -LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS---SLEKDLLAPLIDCTKSYSLSLD 194
            L++ KAD IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL 
Sbjct: 128 PLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLA 187

Query: 195 LFTKGWVPRRN----------RNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRR 254
           LFTKGWVP++           R+ +  H  K    FR G LPL+M+   +AVY S+ +RR
Sbjct: 188 LFTKGWVPKKKKRAGFAWAQPRSGSSTHT-KPFRLFRNGALPLKMTEKTKAVYESL-SRR 247

Query: 255 KLL---SSSDG-----VLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMA 314
           KL    S+ DG     VL++ +V VSQDG G+F NIT A+ AAPNN+  S G+FLIY+ A
Sbjct: 248 KLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTA 307

Query: 315 GVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITI 374
           G+Y+EY+S+   K+Y++MIGDGINQT++TGNRSV DGWTTFNSATFAV A  F+AVNIT 
Sbjct: 308 GIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITF 367

Query: 375 QNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNA 434
           +NTAG  K QAVALRSGAD  +FYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNA
Sbjct: 368 RNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNA 427

Query: 435 AVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTY 494
           AVVFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NC+I+   +L  S++Y  KTY
Sbjct: 428 AVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTY 487

Query: 495 LGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-LSTLYYGEYNDTGGGSDTKNRVTWA 554
           LGRPWK+YSRTVYMQ++IDGFV P GW  W G + LSTLYY EYN+TG GS+T NRVTW 
Sbjct: 488 LGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWP 547

Query: 555 GYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 565
           GYHVI N+TDAANFT++   +   W+  T VPYT G +
Sbjct: 548 GYHVI-NSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Chy4G069330 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.7e-126
Identity = 255/542 (47.05%), Postives = 344/542 (63.47%), Query Frame = 0

Query: 41  CKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSI 100
           CK T  P  C T+L   +S   + Y  G+F+I++ L +A+R   +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 101 ----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLD 160
               IGA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 161 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGK----KHLQ 220
           GL  V + S     + +P+ + T+ YS+SL L +      RN  R     GK     +  
Sbjct: 203 GL--VEAKSGFAAAIGSPMGNLTRLYSISLGLVSHAL--NRNLKRFKASKGKILGGGNST 262

Query: 221 FRKGPLPL------RMSRHDRAVYNSVANRRKLLSSSDG-VLVNGVVVVSQDGHGDFLNI 280
           +R+ PL        +   +D+    +  N  +L  +S G +LV+  V+V      +F  I
Sbjct: 263 YRE-PLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTI 322

Query: 281 TDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVAD 340
           TDAI AAPNN+   DGYF+IY   GVY+EY+ VP  KK L+++GDGIN+TIITGN +V D
Sbjct: 323 TDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVD 382

Query: 341 GWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLY 400
           GWTT+N ++FAV  E FMAV++T +NTAG  K QAVALR+ A+   FY CSFEGYQDTLY
Sbjct: 383 GWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLY 442

Query: 401 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGT 460
            HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR DPNQNTG 
Sbjct: 443 VHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGI 502

Query: 461 SIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-L 520
           SI NC+I+A P+LAA       T+LGRPWK YSRTV+MQ++I   V P GW  W G   L
Sbjct: 503 SIINCTIKAAPDLAAEPK-SAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGL 562

Query: 521 STLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGG 565
            T+YYGEY++ G G++T  RV W GY+++N A +A NFT+ NF +GD WLP T +P+ GG
Sbjct: 563 DTIYYGEYSNFGPGANTNQRVQWLGYNLLNLA-EAMNFTVYNFTMGDTWLPQTDIPFYGG 616

BLAST of Chy4G069330 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.4e-120
Identity = 246/549 (44.81%), Postives = 331/549 (60.29%), Query Frame = 0

Query: 36  PTKAICKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSST 95
           P    CK T  P  C T+L   +S   + Y  G+F++++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 96  LPNSI----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 155
            P +     + A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQ 202

Query: 156 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKHL 215
            TCLDGL    SG +    +  PL + T+ YS+SL L +       NRN    + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA----LNRN-LKRYKGSKGK 262

Query: 216 QFRKGPLPLR----------MSRHDRAVYNSVANRR--KLLSSSDG-VLVNGVVVVSQDG 275
            F  G  P+R              D+      ANR   +L  +S G +LV   V V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 276 HGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIIT 335
             +F  IT+A+ AAPN++    GYF+IY  AG+Y+EYV + +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 336 GNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFE 395
           GN S  DGWTT+NS+TFAV  + F+AV++T +NTAG  K QAVA+R+ AD   FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 396 GYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSD 455
           GYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 456 PNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDP 515
           PNQ TG SI NC+I A P+LAA       T+LGRPWK YSRTVY+Q++I   V P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAADPK-STMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 516 WTGQY-LSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPT 565
           W G   L T+ YGEY++ G G+DT  RV W+GY ++ N   A NFT+ NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621

BLAST of Chy4G069330 vs. ExPASy TrEMBL
Match: A0A0A0LHM0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G890080 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 552/565 (97.70%), Postives = 557/565 (98.58%), Query Frame = 0

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPLLASFLILLTLFTSSFASDDSLT FFPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSS 240
           SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKL SS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 S-DGVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSK 300
           S DGVLVNGVVVVSQDG GDFLNITDAINAAPNNSLASDGYFLIYI AGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTV 480
           LP PGQANMITAQGRSDPNQNTGTSIHNC+IRATPELAAS+SYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGQYLSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAAN 540
           YMQTFIDGFVNPKGWDPWTG+YLSTLYYGEYN+TGGGSDTKNRVTWAGYHVINN TDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYTGGFV 565
           FTISNFLVGDAWLPPTWVPY GGFV
Sbjct: 541 FTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of Chy4G069330 vs. ExPASy TrEMBL
Match: A0A5A7TAP2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005740 PE=3 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.9e-309
Identity = 534/565 (94.51%), Postives = 546/565 (96.64%), Query Frame = 0

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPL   FLILLTLFTSSFASDDSLT  FPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKA+RFLDLIEKHLQNSSTLP SIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSS 240
           SLSLDLFTKGWVPR NRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKL SS
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 S-DGVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSK 300
           S DGVLVNG+ VVSQDG GDFLNITDAINAAPNNSLA++GYFLIYI AGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTV 480
           LP P QANMITAQGRSDPNQNTGTSIHNC+I+ATP+LAAS+S+MNKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGQYLSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAAN 540
           YMQTFIDGFVNPKGWDPW+G+YLSTLYYGEYN+TGGGSDTKNRV W GYHVINNATDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYTGGFV 565
           FTISNFLVGD WLPPT VPYTGGFV
Sbjct: 541 FTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of Chy4G069330 vs. ExPASy TrEMBL
Match: A0A1S4E6G9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.9e-309
Identity = 534/565 (94.51%), Postives = 546/565 (96.64%), Query Frame = 0

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPL   FLILLTLFTSSFASDDSLT  FPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKA+RFLDLIEKHLQNSSTLP SIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSS 240
           SLSLDLFTKGWVPR NRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKL SS
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 S-DGVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSK 300
           S DGVLVNG+ VVSQDG GDFLNITDAINAAPNNSLA++GYFLIYI AGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTV 480
           LP P QANMITAQGRSDPNQNTGTSIHNC+I+ATP+LAAS+S+MNKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGQYLSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAAN 540
           YMQTFIDGFVNPKGWDPW+G+YLSTLYYGEYN+TGGGSDTKNRV W GYHVINNATDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYTGGFV 565
           FTISNFLVGD WLPPT VPYTGGFV
Sbjct: 541 FTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of Chy4G069330 vs. ExPASy TrEMBL
Match: A0A1S3CQK8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1)

HSP 1 Score: 1031.9 bits (2667), Expect = 9.7e-298
Identity = 509/537 (94.79%), Postives = 521/537 (97.02%), Query Frame = 0

Query: 29  DSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHL 88
           DSLT  FPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKA+RFLDLIEKHL
Sbjct: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68

Query: 89  QNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 148
           QNSSTLP SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128

Query: 149 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKH 208
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRTLEHPGKKH
Sbjct: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188

Query: 209 LQFRKGPLPLRMSRHDRAVYNSVANRRKLLSSS-DGVLVNGVVVVSQDGHGDFLNITDAI 268
           LQFRKGPLPLRMSRHDRAVYNSVANRRKL SSS DGVLVNG+ VVSQDG GDFLNITDAI
Sbjct: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248

Query: 269 NAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 328
           NAAPNNSLA++GYFLIYI AGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308

Query: 329 FNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 388
           FNSATFAVAAEGFMAVNIT QNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL
Sbjct: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368

Query: 389 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHN 448
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP P QANMITAQGRSDPNQNTGTSIHN
Sbjct: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428

Query: 449 CSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQYLSTLYY 508
           C+I+ATP+LAAS+S+MNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPW+G+YLSTLYY
Sbjct: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488

Query: 509 GEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 565
           GEYN+TGGGSDTKNRV W GYHVINNATDAANFTISNFLVGD WLPPT VPYTGGFV
Sbjct: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 545

BLAST of Chy4G069330 vs. ExPASy TrEMBL
Match: A0A6J1J323 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480894 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 2.0e-274
Identity = 469/541 (86.69%), Postives = 504/541 (93.16%), Query Frame = 0

Query: 25  FASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLI 84
           F  D SL   F  KAICK TSNPSYCI+VL++S  GN+Y+SGRFS+RRSL+KAT FL+LI
Sbjct: 5   FHPDGSLNDLFLAKAICKFTSNPSYCISVLQKS-SGNVYESGRFSMRRSLAKATTFLNLI 64

Query: 85  EKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSA 144
           EKHLQNSSTLP S+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+KLTYSKADYIQSLLSA
Sbjct: 65  EKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSA 124

Query: 145 ILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHP 204
           ILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP RNRNRT +HP
Sbjct: 125 ILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP-RNRNRTRDHP 184

Query: 205 GKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSSS-DGVLVNGVVVVSQDGHGDFLNI 264
             KHL+FRKGPLPLRMS+HDRAVYNSVANRR+L SSS DG+LVNG+VVVSQDG G+FL I
Sbjct: 185 VTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTI 244

Query: 265 TDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVAD 324
           T+A+NAAPNNSLA +GYFLIYI AGVYQEYVS+PSKKKYLLMIGDGINQTIITGN+SVAD
Sbjct: 245 TEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVAD 304

Query: 325 GWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLY 384
           GWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAVA+RSGADM +FYSCSFEGYQDTLY
Sbjct: 305 GWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLY 364

Query: 385 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGT 444
           THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP  GQ NMITAQGRSDPNQNTGT
Sbjct: 365 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGT 424

Query: 445 SIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQYLS 504
           SIHNC+IRAT +LAAS SYMNKTYLGRPWKQYSRTVYMQTFIDGFVNP GWDPW+G+YLS
Sbjct: 425 SIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEYLS 484

Query: 505 TLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGF 564
           TLYY EYN+TG GS+TKNRVTW GYHVI+NATDA+NFT+SNFLVGDAWLPPT VPYTGGF
Sbjct: 485 TLYYAEYNNTGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGF 543

BLAST of Chy4G069330 vs. NCBI nr
Match: XP_031738568.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738569.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738570.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >KGN60252.1 hypothetical protein Csa_001115 [Cucumis sativus])

HSP 1 Score: 1108 bits (2867), Expect = 0.0
Identity = 552/565 (97.70%), Postives = 557/565 (98.58%), Query Frame = 0

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPLLASFLILLTLFTSSFASDDSLT FFPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSS 240
           SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKL SS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 SD-GVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSK 300
           SD GVLVNGVVVVSQDG GDFLNITDAINAAPNNSLASDGYFLIYI AGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTV 480
           LP PGQANMITAQGRSDPNQNTGTSIHNC+IRATPELAAS+SYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGQYLSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAAN 540
           YMQTFIDGFVNPKGWDPWTG+YLSTLYYGEYN+TGGGSDTKNRVTWAGYHVINN TDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYTGGFV 564
           FTISNFLVGDAWLPPTWVPY GGFV
Sbjct: 541 FTISNFLVGDAWLPPTWVPYMGGFV 565

BLAST of Chy4G069330 vs. NCBI nr
Match: XP_016903565.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo] >KAA0038671.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa] >TYK31280.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 534/565 (94.51%), Postives = 546/565 (96.64%), Query Frame = 0

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPL   FLILLTLFTSSFASDDSLT  FPTKAICKLTSNPSYCITVLKQSRDG
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKA+RFLDLIEKHLQNSSTLP SIIGALKDCQYLAQLNMNFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSS 240
           SLSLDLFTKGWVPR NRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKL SS
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 SD-GVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSK 300
           SD GVLVNG+ VVSQDG GDFLNITDAINAAPNNSLA++GYFLIYI AGVYQEYVSVPSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTV 480
           LP P QANMITAQGRSDPNQNTGTSIHNC+I+ATP+LAAS+S+MNKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGQYLSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAAN 540
           YMQTFIDGFVNPKGWDPW+G+YLSTLYYGEYN+TGGGSDTKNRV W GYHVINNATDAAN
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYTGGFV 564
           FTISNFLVGD WLPPT VPYTGGFV
Sbjct: 541 FTISNFLVGDVWLPPTGVPYTGGFV 562

BLAST of Chy4G069330 vs. NCBI nr
Match: XP_038898430.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898431.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898432.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida])

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 514/565 (90.97%), Postives = 534/565 (94.51%), Query Frame = 0

Query: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDG 60
           MACKFLFPPLLASFLILLTLFTSS A  DSLT F P+KAICK TSNPSYCITVLK+S  G
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSSAEYDSLTNFPPSKAICKFTSNPSYCITVLKKSTGG 60

Query: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120
           NIYDSGRFSIRRSLSKA+RF DLIEKHLQNSSTLP SI GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFFDLIEKHLQNSSTLPKSITGALTDCQYLAQLNINFLSNSF 120

Query: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180
           RAVNGTD+KLTYSKADYIQSLLSAILTNIDTCLDGL TVA+GSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVAAGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSS 240
           SLSLDLFTKGWVPRRNRNRTL+HPGKKHLQFRKGPLPLRMSR DRAVY+SVANRRKL SS
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLDHPGKKHLQFRKGPLPLRMSRRDRAVYDSVANRRKLSSS 240

Query: 241 SD-GVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSK 300
           SD GVLVNG+ VVSQDG GDFLNITDAINAAPNNSLA++GYFLIYI AGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGIAVVSQDGKGDFLNITDAINAAPNNSLAANGYFLIYISAGVYQEYVSIPSK 300

Query: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360
           KKYLLMIGDGINQTIITGN SV DGWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNHSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTV 480
           LP PGQANMITAQGRSDPNQNTGTSIHNC+IRAT +LAAS+SYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCAIRATSDLAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFVNPKGWDPWTGQYLSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAAN 540
           YMQTFIDGFVNP GWDPWTG+YLSTLYYGEYN+TG GS+TKNRV WAGYHVINNATDAAN
Sbjct: 481 YMQTFIDGFVNPSGWDPWTGEYLSTLYYGEYNNTGDGSNTKNRVNWAGYHVINNATDAAN 540

Query: 541 FTISNFLVGDAWLPPTWVPYTGGFV 564
           FT+SNFLVGD WLP T VPYTG F+
Sbjct: 541 FTVSNFLVGDVWLPQTCVPYTGAFI 565

BLAST of Chy4G069330 vs. NCBI nr
Match: XP_008466170.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X2 [Cucumis melo] >XP_008466171.1 PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X2 [Cucumis melo])

HSP 1 Score: 1029 bits (2660), Expect = 0.0
Identity = 509/537 (94.79%), Postives = 521/537 (97.02%), Query Frame = 0

Query: 29  DSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHL 88
           DSLT  FPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKA+RFLDLIEKHL
Sbjct: 9   DSLTNIFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKASRFLDLIEKHL 68

Query: 89  QNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 148
           QNSSTLP SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN
Sbjct: 69  QNSSTLPKSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTN 128

Query: 149 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKH 208
           IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRTLEHPGKKH
Sbjct: 129 IDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRWNRNRTLEHPGKKH 188

Query: 209 LQFRKGPLPLRMSRHDRAVYNSVANRRKLLSSSD-GVLVNGVVVVSQDGHGDFLNITDAI 268
           LQFRKGPLPLRMSRHDRAVYNSVANRRKL SSSD GVLVNG+ VVSQDG GDFLNITDAI
Sbjct: 189 LQFRKGPLPLRMSRHDRAVYNSVANRRKLSSSSDDGVLVNGIAVVSQDGEGDFLNITDAI 248

Query: 269 NAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 328
           NAAPNNSLA++GYFLIYI AGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT
Sbjct: 249 NAAPNNSLAANGYFLIYITAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTT 308

Query: 329 FNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 388
           FNSATFAVAAEGFMAVNIT QNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL
Sbjct: 309 FNSATFAVAAEGFMAVNITFQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSL 368

Query: 389 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHN 448
           RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP P QANMITAQGRSDPNQNTGTSIHN
Sbjct: 369 RQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPGPRQANMITAQGRSDPNQNTGTSIHN 428

Query: 449 CSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQYLSTLYY 508
           C+I+ATP+LAAS+S+MNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPW+G+YLSTLYY
Sbjct: 429 CTIQATPDLAASSSHMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWSGEYLSTLYY 488

Query: 509 GEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 564
           GEYN+TGGGSDTKNRV W GYHVINNATDAANFTISNFLVGD WLPPT VPYTGGFV
Sbjct: 489 GEYNNTGGGSDTKNRVIWPGYHVINNATDAANFTISNFLVGDVWLPPTGVPYTGGFV 545

BLAST of Chy4G069330 vs. NCBI nr
Match: KAG6608276.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 954 bits (2467), Expect = 0.0
Identity = 469/541 (86.69%), Postives = 505/541 (93.35%), Query Frame = 0

Query: 25  FASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLI 84
           F  D SL   FP KAICK TSNPSYCI+VL++S  GN+Y+SGRFS+RRSLSKAT FL+LI
Sbjct: 5   FHPDGSLNGLFPAKAICKFTSNPSYCISVLQKS-SGNVYESGRFSMRRSLSKATTFLNLI 64

Query: 85  EKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSA 144
           EKHLQNSSTLP S+ G LKDCQYLAQLN+NFLSNSFRAVNGTD+KLTYSKADYIQSLLSA
Sbjct: 65  EKHLQNSSTLPKSVTGGLKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSA 124

Query: 145 ILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHP 204
           ILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR NRNRT +HP
Sbjct: 125 ILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPR-NRNRTRDHP 184

Query: 205 GKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLLSSSD-GVLVNGVVVVSQDGHGDFLNI 264
             KHL+FRKGPLPLRMS+HDRAVYNSVANRR+L SSSD G+LVNG+VVVSQDG G+FL I
Sbjct: 185 VTKHLRFRKGPLPLRMSKHDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTI 244

Query: 265 TDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVAD 324
           T+A+NAAPNNSLA +GYFLIYI AGVYQEYVS+PSKKKYLLMIGDGINQTIITGN+SVAD
Sbjct: 245 TEAVNAAPNNSLAVNGYFLIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVAD 304

Query: 325 GWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLY 384
           GWTTFNSATFAVAAEGFMAVNIT QNTAGAIKGQAVA+RSGADM +FYSCSFEGYQDTLY
Sbjct: 305 GWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLY 364

Query: 385 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGT 444
           THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLP  GQ NMITAQGRSDPNQNTGT
Sbjct: 365 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGT 424

Query: 445 SIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQYLS 504
           SIHNC+IRAT +LAAS SYMNKTYLGRPWKQYSRTVYMQTFIDGF+NP GWDPW+G+YLS
Sbjct: 425 SIHNCTIRATSDLAASKSYMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLS 484

Query: 505 TLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGF 564
           TLYY EYN++G GS+TKNRVTW GYHVI+NATDA+NFT+SNFLVGDAWLPPT VPYTGGF
Sbjct: 485 TLYYAEYNNSGAGSNTKNRVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGF 543

BLAST of Chy4G069330 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 625.9 bits (1613), Expect = 3.1e-179
Identity = 313/530 (59.06%), Postives = 396/530 (74.72%), Query Frame = 0

Query: 40  ICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHL-QNSSTLPNSI 99
           IC+   +PSYC +VL  ++ G+IY  GR S+RRSLS+A RF+ +I+  L +       S 
Sbjct: 38  ICRFAPDPSYCRSVL-PNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGKVAAKST 97

Query: 100 IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNT 159
           +GAL+DC++LA L M++L +S +  + T + L+ S+A+ + + LSA +TN  TCL+GL +
Sbjct: 98  VGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTCLEGLKS 157

Query: 160 VASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPG---KKHLQFRKGP 219
            AS + L  D    L + TK Y +SL LF+KGWVPRR R+R +  P    KK   FR G 
Sbjct: 158 TASENGLSGD----LFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGK 217

Query: 220 LPLRMSRHDRAVYNSVANRRKLLSSSDGVLVNGVVVVSQDGHGDFLNITDAINAAPNNSL 279
           LPL+M+   RAVYN+V  R+ L S +D V V+ +V V Q+G G+F  I  AI AAPN + 
Sbjct: 218 LPLKMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTD 277

Query: 280 ASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAV 339
            S+GYFLIY+ AG+Y+EYV VP  K+Y++MIGDGINQT+ITGNRSV DGWTTFNSATF +
Sbjct: 278 GSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFIL 337

Query: 340 AAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECD 399
           +   F+ VNITI+NTAG  KGQAVALRSG D+ VFYSCSFE YQDTLYTHSLRQF+RECD
Sbjct: 338 SGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECD 397

Query: 400 IYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPE 459
           +YGTVDFIFGNAAVV QNCN+YPR P  GQ+N +TAQGR+DPNQNTGT+IH C+IR   +
Sbjct: 398 VYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADD 457

Query: 460 LAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-LSTLYYGEYNDTG 519
           LA S +Y  KTYLGRPWK+YSRTV MQT+IDGF+ P GW+ W+G + LSTLYY EYN+TG
Sbjct: 458 LATS-NYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTG 517

Query: 520 GGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 565
            GSDT NRVTW GYHVI NATDA+NFT++NFLVG+ W+  T VP+ GG +
Sbjct: 518 PGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Chy4G069330 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 604.4 bits (1557), Expect = 9.6e-173
Identity = 311/574 (54.18%), Postives = 413/574 (71.95%), Query Frame = 0

Query: 10  LLASFLILLTLFTS---SFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSG 69
           +L+  L L+TLF S    F +  +L     +  ICK T +P +C +V  Q+  G++ + G
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYG 60

Query: 70  RFSIRRSLSKATRFLDLIEKHL-QNSSTLPNSIIGALKDCQYLAQLNMNFLSNSFRAVNG 129
           RFS+R+SL+++ +F   I+++L +N++ L  S +GAL+DC+YLA L  ++L  SF  VN 
Sbjct: 61  RFSLRKSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNI 120

Query: 130 T--DRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS-SLEKDLLAPLIDCTKSYSL 189
           T   + L++SKAD IQ+LLSA LTN  TCLDG+NT AS S ++   +  PLI+ TK +S+
Sbjct: 121 TTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSV 180

Query: 190 SLDLFTKGWVPRRNR---NRTLEHPGKKH------LQFRKGPLPLRMSRHDRAVYNSVAN 249
           SL LFTKGWVP++ +   + +  HP   H        FR G LPL+M+ H RAVY S++ 
Sbjct: 181 SLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSR 240

Query: 250 RRKLLSSSD--GVLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQ 309
           R+     +D   VLV+ +V V+Q+G G+F  IT+A+N+APN +  + GYF+IY+ +GVY+
Sbjct: 241 RKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYE 300

Query: 310 EYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTA 369
           E V +   K+YL+MIGDGIN+T++TGNR+V DGWTTFNSATFAV +  F+AVN+T +NTA
Sbjct: 301 ENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTA 360

Query: 370 GAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVF 429
           G  K QAVA+RS AD+ +FYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVF
Sbjct: 361 GPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVF 420

Query: 430 QNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRP 489
           Q+CN+YPR P   Q N ITAQGR+DPNQNTG SIHNC+I+   +L  S++Y  KTYLGRP
Sbjct: 421 QDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDL-VSSNYTVKTYLGRP 480

Query: 490 WKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-LSTLYYGEYNDTGGGSDTKNRVTWAGYHV 549
           WK+YSRTV+MQ++ID  V P GW  W G + LSTLYY EYN+TG GS T +RV W GYHV
Sbjct: 481 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 540

Query: 550 INNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 565
           I N+TDA NFT+ NFL+GD W+  + VPY  G +
Sbjct: 541 I-NSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Chy4G069330 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 602.8 bits (1553), Expect = 2.8e-172
Identity = 323/578 (55.88%), Postives = 410/578 (70.93%), Query Frame = 0

Query: 15  LILLTLFTSSFASDDSLTTFFPTKAICKLTSNPSYCITVLKQSRDGNIYDSGRFSIRRSL 74
           LI L+ F  S  +  S  T   +  ICK T +P YC +V   S+ GN+   G FSIR+SL
Sbjct: 8   LITLSFFLQSVLA--SSQTLSNSSTICKTTPDPKYCKSVFPHSQ-GNVQQYGCFSIRKSL 67

Query: 75  SKATRFLDLIEKHLQNSSTLPN-SIIGALKDCQYLAQLNMNFLSNSFRAVNGTDRK---- 134
           S++ +F+  ++++++ ++ L   ++I AL+DC++LA L M++L  SF  VN T  K    
Sbjct: 68  SQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFK 127

Query: 135 -LTYSKADYIQSLLSAILTNIDTCLDGLNTVASGS---SLEKDLLAPLIDCTKSYSLSLD 194
            L++ KAD IQ+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL 
Sbjct: 128 PLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLA 187

Query: 195 LFTKGWVPRRN----------RNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRR 254
           LFTKGWVP++           R+ +  H  K    FR G LPL+M+   +AVY S+ +RR
Sbjct: 188 LFTKGWVPKKKKRAGFAWAQPRSGSSTHT-KPFRLFRNGALPLKMTEKTKAVYESL-SRR 247

Query: 255 KLL---SSSDG-----VLVNGVVVVSQDGHGDFLNITDAINAAPNNSLASDGYFLIYIMA 314
           KL    S+ DG     VL++ +V VSQDG G+F NIT A+ AAPNN+  S G+FLIY+ A
Sbjct: 248 KLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTA 307

Query: 315 GVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITI 374
           G+Y+EY+S+   K+Y++MIGDGINQT++TGNRSV DGWTTFNSATFAV A  F+AVNIT 
Sbjct: 308 GIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITF 367

Query: 375 QNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNA 434
           +NTAG  K QAVALRSGAD  +FYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNA
Sbjct: 368 RNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNA 427

Query: 435 AVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGTSIHNCSIRATPELAASTSYMNKTY 494
           AVVFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NC+I+   +L  S++Y  KTY
Sbjct: 428 AVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDL-VSSNYTVKTY 487

Query: 495 LGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-LSTLYYGEYNDTGGGSDTKNRVTWA 554
           LGRPWK+YSRTVYMQ++IDGFV P GW  W G + LSTLYY EYN+TG GS+T NRVTW 
Sbjct: 488 LGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWP 547

Query: 555 GYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGGFV 565
           GYHVI N+TDAANFT++   +   W+  T VPYT G +
Sbjct: 548 GYHVI-NSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Chy4G069330 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-127
Identity = 255/542 (47.05%), Postives = 344/542 (63.47%), Query Frame = 0

Query: 41  CKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSI 100
           CK T  P  C T+L   +S   + Y  G+F+I++ L +A+R   +I  + +   + P S 
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 101 ----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLD 160
               IGA+ DC  L++L++N+L      +  T + +T +  +++ SLLS ++TN  TCLD
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGVVTNQQTCLD 202

Query: 161 GLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGK----KHLQ 220
           GL  V + S     + +P+ + T+ YS+SL L +      RN  R     GK     +  
Sbjct: 203 GL--VEAKSGFAAAIGSPMGNLTRLYSISLGLVSHAL--NRNLKRFKASKGKILGGGNST 262

Query: 221 FRKGPLPL------RMSRHDRAVYNSVANRRKLLSSSDG-VLVNGVVVVSQDGHGDFLNI 280
           +R+ PL        +   +D+    +  N  +L  +S G +LV+  V+V      +F  I
Sbjct: 263 YRE-PLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDNFTTI 322

Query: 281 TDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIITGNRSVAD 340
           TDAI AAPNN+   DGYF+IY   GVY+EY+ VP  KK L+++GDGIN+TIITGN +V D
Sbjct: 323 TDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNHNVVD 382

Query: 341 GWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFEGYQDTLY 400
           GWTT+N ++FAV  E FMAV++T +NTAG  K QAVALR+ A+   FY CSFEGYQDTLY
Sbjct: 383 GWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQDTLY 442

Query: 401 THSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSDPNQNTGT 460
            HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR DPNQNTG 
Sbjct: 443 VHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQNTGI 502

Query: 461 SIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDPWTGQY-L 520
           SI NC+I+A P+LAA       T+LGRPWK YSRTV+MQ++I   V P GW  W G   L
Sbjct: 503 SIINCTIKAAPDLAAEPK-SAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGTIGL 562

Query: 521 STLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPTWVPYTGG 565
            T+YYGEY++ G G++T  RV W GY+++N A +A NFT+ NF +GD WLP T +P+ GG
Sbjct: 563 DTIYYGEYSNFGPGANTNQRVQWLGYNLLNLA-EAMNFTVYNFTMGDTWLPQTDIPFYGG 616

BLAST of Chy4G069330 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 434.9 bits (1117), Expect = 1.0e-121
Identity = 246/549 (44.81%), Postives = 331/549 (60.29%), Query Frame = 0

Query: 36  PTKAICKLTSNPSYCITVLK--QSRDGNIYDSGRFSIRRSLSKATRFLDLIEKHLQNSST 95
           P    CK T  P  C T+L   +S   + Y  G+F++++ L +A R   +I +  Q    
Sbjct: 83  PPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQRVEA 142

Query: 96  LPNSI----IGALKDCQYLAQLNMNFLSNSFRAVNGTDRKLTYSKADYIQSLLSAILTNI 155
            P +     + A+ DC  LA+L++ +L      +   +  +T +  D + SLL  ++TN 
Sbjct: 143 DPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLGGVVTNQ 202

Query: 156 DTCLDGLNTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRRNRNRTLEHPGKKHL 215
            TCLDGL    SG +    +  PL + T+ YS+SL L +       NRN    + G K  
Sbjct: 203 QTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHA----LNRN-LKRYKGSKGK 262

Query: 216 QFRKGPLPLR----------MSRHDRAVYNSVANRR--KLLSSSDG-VLVNGVVVVSQDG 275
            F  G  P+R              D+      ANR   +L  +S G +LV   V V    
Sbjct: 263 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 322

Query: 276 HGDFLNITDAINAAPNNSLASDGYFLIYIMAGVYQEYVSVPSKKKYLLMIGDGINQTIIT 335
             +F  IT+A+ AAPN++    GYF+IY  AG+Y+EYV + +KK+ +++IGDGIN+TII+
Sbjct: 323 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 382

Query: 336 GNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAVALRSGADMCVFYSCSFE 395
           GN S  DGWTT+NS+TFAV  + F+AV++T +NTAG  K QAVA+R+ AD   FY CSFE
Sbjct: 383 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 442

Query: 396 GYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPSPGQANMITAQGRSD 455
           GYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+D
Sbjct: 443 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 502

Query: 456 PNQNTGTSIHNCSIRATPELAASTSYMNKTYLGRPWKQYSRTVYMQTFIDGFVNPKGWDP 515
           PNQ TG SI NC+I A P+LAA       T+LGRPWK YSRTVY+Q++I   V P GW  
Sbjct: 503 PNQKTGISIINCTIGAAPDLAADPK-STMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 562

Query: 516 WTGQY-LSTLYYGEYNDTGGGSDTKNRVTWAGYHVINNATDAANFTISNFLVGDAWLPPT 565
           W G   L T+ YGEY++ G G+DT  RV W+GY ++ N   A NFT+ NF +GD WLP T
Sbjct: 563 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQT 621