Chy4G068710 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G068710
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Aminoacyl-tRNA hydrolase
Location	chrH04: 1367647 .. 1370097 (+)
RNA-Seq Expression	Chy4G068710
Synteny	Chy4G068710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGGGTGCTTCTAAGTCTTCCAGAAAGGTATTCAATTTCATGGGTTGCATTCCTTCTTCCCTCTTTACCCCCTCTTGTTGATGTTTGAATTAATTTTTCTCGTTCTTTCTTTCCGTATTGTTAGAAGCAGCAAGTGAAGCCAGAAAAAGAATGGCTGGCGGTGAGTTTCAAACCCGAGAACTTCATTCCAGGCCTCGTAATTGGTTTCATTATTGGGTTGTTTTTGGATTTATCAAAACCCAGTAAAGGTAATGCTAAGAAGGGCTATTTCCTGCCGTCCAAGTACCAACAGCTTTCTCCAGCCTCAAATAACGGTGATCAAGAGCTCAAATTGGTTAGTGTTCGACTTTACTAGAATTTCTCGATTTCTGTCGAGTTGGAGGTGATTTCTCTACATGTTTGTAATGGATTATGATCACTAAATTAGAATGAAATGAAAGAACAAAATAAGAAGTTTTTAGTTCAACTGTCTACGATAGAAGGGTACGAGGTTTCCTGTTTAATGTCATTGAATATGGTTTTCTATGGCTCAATTTTGAGGCGTGAATTGCTTGTAAGATAGAATAGTTTAGGGCCTATTTGAGGTAGTTGTATAATTATATTGCGAACAAAATTAGCTTTCGACAATCGTTAAGCAGAGTTTTAGGTTCTGTGCTTACCCCGTCTTTGCTGTTGCCATCAAGTATCCCTGTAGTCTGACTGGCAGAACCTAAAAGGCGAATCAATTAGTCTGAGGATCAAAAAGGGCGGGTGAAATATGATTTAGCAGTTAGACAAGTTATCTTTACTGTTATGAAATCAAGATAAGAAAAAAACGTAGTAGAGAATCAACACAGAAAGAGCCAAAATCCTAAATAACTTATATAGGACAAATACGGAAGTAACTTAGATTTTGAGGGCATTCCCAGTGAGCCCTCTTACTTGGTCATTTGAAGCGCCAAATAATAAATTTAAGACGATCCAACATTTACTTTGCAAACTTGGTTATGTTTAGTTCCAAAAATCTTGAAATGTTGTAGTATGGCTTATGTAGATTTCCTTCCCTAAGCATAGAAACTAGAAAGGGAACTACACTGACAAATTGACAATCACACCAAGTCCTTGTGTATAACTTGTACAATGCGTCTTGCTGTGAAGAATCATCTGACATTCTTACTTTTCAATTGAAGGTCCTTGTCGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCGTAAGATTTAAGATGCTGAATTCCATACTTGTTACATGCGCATTTGATTTTTGTTTCTATTTACATTGGTTGGTTTCTCACTTCACGTCTTCCTGCCATTCAAGCTAAATATATTTGCATGTTTTTCTGTCAAATTGTGAAGATGCCGCTACTGGCTTGTATGCAGAACTAATGCAAAGGTATTCCTTATACTATACTATTTATGAATATCTGCTTTGATCAAACCATATACGCAGCTAATTCTTCATCCTTTTCTCTTTTCTGGATATCTATGCCCATTTCTAATATCTAGTGCAGCCACCGAAACCTTTTGAGACAGTGGGAACTATGTGGGCAGCCGAAAATAGTTGTAACATGCAAGAATCAACACGAAATGTAAGCAGGTGAATCCTTTCTCCAAAAATGTAATCGGTAATAAACACATGAAACCAAAACCTTACGGTTTTTGGAAAGACTTTGTAGGTCTGTGTGTTGAGGACTCTGGGAGGAGGAATGAGAGCACGGGGGAAGGGTTATTTAATAAAGTTACACATTTTTTCGTTGTTAAATTTACTTATTTTCTCAAATCAGGAATAAACTGAAGGATTCAGCTGAGAATATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTACAAACTTTGTTCTCCACTTGTCTGCTTGGATGAATTTACAAGCATTTTGATTAATGCATGAGTAATGGTGGGGTAATTGGGTGCAACAATGCTTGACATGTTTTATTAATGTCTATATTTTGGCATAGTCAAACGATTCTACACATGTTATGCTGACATACGTTGGCATTACATTGTTTTATGATTGTTAGGCATCAGAAAGTTAATTTTATTGGATCTCCTAAGCGAAAAAAATAACTGTTTAGTGGTTCAGCGTTGTATAGAGTCCTCGGTTTTGTAGTTGATGGAAAGGTTCACTTGGCTCTTTGAAGAATCGATTTTTTCCAAGCCCAAATTTTTATGCAATAATCATTTTGATGAACTTCTAGTTCAAAGTGCCTATAATTGGACACCTCAAGAAACATGTTTGTTAACATTTCACTAAGTACATATAGTTCTCCGGAACTTGGCTGCTGGCATCCTGAAATCAGTTCAATGATGTGCAGGTTTCGGCTGGATCGAAGACTGTTCTTGCCATTGGACCTGGTAATGCTTCTTTACCCCATGATAGAATTCCGTGGTTGCCTCGTTGA

mRNA sequence

ATGTTGGGTGCTTCTAAGTCTTCCAGAAAGAAGCAGCAAGTGAAGCCAGAAAAAGAATGGCTGGCGGTGAGTTTCAAACCCGAGAACTTCATTCCAGGCCTCGTAATTGGTTTCATTATTGGGTTGTTTTTGGATTTATCAAAACCCAGTAAAGGTAATGCTAAGAAGGGCTATTTCCTGCCGTCCAAGTACCAACAGCTTTCTCCAGCCTCAAATAACGGTGATCAAGAGCTCAAATTGGTCCTTGTCGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCATGCCGCTACTGGCTTGTATGCAGAACTAATGCAAAGCCACCGAAACCTTTTGAGACAGTGGGAACTATGTGGGCAGCCGAAAATAGTTGTAACATGCAAGAATCAACACGAAATGAATAAACTGAAGGATTCAGCTGAGAATATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTTTCGGCTGGATCGAAGACTGTTCTTGCCATTGGACCTGGTAATGCTTCTTTACCCCATGATAGAATTCCGTGGTTGCCTCGTTGA

Coding sequence (CDS)

ATGTTGGGTGCTTCTAAGTCTTCCAGAAAGAAGCAGCAAGTGAAGCCAGAAAAAGAATGGCTGGCGGTGAGTTTCAAACCCGAGAACTTCATTCCAGGCCTCGTAATTGGTTTCATTATTGGGTTGTTTTTGGATTTATCAAAACCCAGTAAAGGTAATGCTAAGAAGGGCTATTTCCTGCCGTCCAAGTACCAACAGCTTTCTCCAGCCTCAAATAACGGTGATCAAGAGCTCAAATTGGTCCTTGTCGTTAGGCAAGATCTGAAGATGGGTTCTGGAAAAATTGCATCTCAATGTGCTCATGCCGCTACTGGCTTGTATGCAGAACTAATGCAAAGCCACCGAAACCTTTTGAGACAGTGGGAACTATGTGGGCAGCCGAAAATAGTTGTAACATGCAAGAATCAACACGAAATGAATAAACTGAAGGATTCAGCTGAGAATATTGGCCTTCCTACTTTTGTTGTTGCTGATGCTGGACGCACACAGGTTTCGGCTGGATCGAAGACTGTTCTTGCCATTGGACCTGGTAATGCTTCTTTACCCCATGATAGAATTCCGTGGTTGCCTCGTTGA

Protein sequence

MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFLPSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNASLPHDRIPWLPR*

Homology

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BLAST of Chy4G068710 vs. ExPASy Swiss-Prot
Match: Q9Y3E5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.4e-26
Identity = 65/151 (43.05%), Postives = 91/151 (60.26%), Query Frame = 0


Query: 33  GLVIGFIIGLFLDLS-KPSKGNAKKGYFLPSKYQQLSPASNNGDQ-ELKLVLVVRQDLKM 92
           GL +G   G+ L  S +   G   K     +     S AS  GD  E K++LVVR DLKM
Sbjct: 18  GLAVGVACGMCLGWSLRVCFGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLKM 77

Query: 93  GSGKIASQCAHAATGLYAELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDSAENIG 152
           G GK+A+QC+HAA   Y ++ + +  +L+QWE CGQPK+VV   ++  +  L   A+ +G
Sbjct: 78  GKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLG 137

Query: 153 LPTFVVADAGRTQVSAGSKTVLAIGPGNASL 182
           L   ++ DAGRTQ++ GS+TVL IGPG A L
Sbjct: 138 LTVSLIQDAGRTQIAPGSQTVLGIGPGPADL 168

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BLAST of Chy4G068710 vs. ExPASy Swiss-Prot
Match: Q3ZBL5 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.9e-25
Identity = 52/105 (49.52%), Postives = 75/105 (71.43%), Query Frame = 0


Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQWELCGQPKIVVTCKNQ 136
           E K++LVVR DLKMG GK+A+QC+HAA   Y ++ + +  LL++WE CGQPK+VV   ++
Sbjct: 64  EYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPELLKEWEYCGQPKVVVKAPDE 123

Query: 137 HEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNASL 182
             + +L   A+ +GL   ++ DAGRTQ++ GS+TVL IGPG A L
Sbjct: 124 ETLVELLTHAKVLGLTVSLIQDAGRTQIAPGSRTVLGIGPGPADL 168

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BLAST of Chy4G068710 vs. ExPASy Swiss-Prot
Match: Q8R2Y8 (Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 7.1e-24
Identity = 50/105 (47.62%), Postives = 71/105 (67.62%), Query Frame = 0


Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQWELCGQPKIVVTCKNQ 136
           E K++LVVR DLKMG GK+A+QC+HAA   Y +  +    +L++WE CGQPK+VV   ++
Sbjct: 66  EYKMILVVRTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDE 125

Query: 137 HEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNASL 182
             + +L   A+ +GL   ++ DAGRTQ+  GS+TVL IGPG   L
Sbjct: 126 DTLIQLLTHAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVEL 170

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BLAST of Chy4G068710 vs. ExPASy Swiss-Prot
Match: O97067 (Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 PE=2 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 2.3e-22
Identity = 66/169 (39.05%), Postives = 89/169 (52.66%), Query Frame = 0


Query: 27  PENFIPGL--VIGFIIGLFLDLSK-PSKGNAKKGYFLPSKYQQLSPASNNGD-------- 86
           P   I GL  ++ F +G    L +  +K +  +G   P  + Q S  S+  +        
Sbjct: 8   PTQIINGLAVMLSFFVGYRYALKRGDAKDSVTEGAATP--FSQESSVSSGSEASVSDKGY 67

Query: 87  ----QELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQWELCGQPKIVV 146
                  K+VLVVR DLKMG GKIA+QC H A G Y   +     LLR WE CG  KI V
Sbjct: 68  GGLNDNFKMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAV 127

Query: 147 TCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 181
             +++ E+  +K  AE   L T ++ DAGRTQ+ A SKTVLA+GP  A+
Sbjct: 128 RVESEAELMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAVGPAAAA 174

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BLAST of Chy4G068710 vs. ExPASy Swiss-Prot
Match: O13830 (Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC19A8.14 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 8.7e-22
Identity = 65/185 (35.14%), Postives = 94/185 (50.81%), Query Frame = 0


Query: 23  VSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFLPSKYQQLSPASNNGDQ------ 82
           +S  P   + G V+GF+IG    ++  S+G + K     S  ++ SP S + D+      
Sbjct: 10  ISSFPAAVLVGAVVGFMIGRKYSVADASRGYSSKNANKASNPEKESPVSVSNDEDSESET 69

Query: 83  --------------------ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRN 142
                               + K+VLVVR DL M  GKIA+QCAHAA   Y        +
Sbjct: 70  ELLDMLKGNSSLAALALAEGQTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPD 129

Query: 143 LLRQWELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGP 182
           L+R WE  GQ KI +  + +  +  L+  A ++GL   V+ DAGRTQ+++GS TVL IGP
Sbjct: 130 LVRIWENAGQAKITLQAQTEETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGP 189

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BLAST of Chy4G068710 vs. ExPASy TrEMBL
Match: A0A5D3E5Y4 (Aminoacyl-tRNA hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005040 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 2.8e-100
Identity = 185/190 (97.37%), Postives = 189/190 (99.47%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 180
           WELCGQPKIVVTCKNQHEMNKLKD+AENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 180

Query: 181 LPHDRIPWLP 191
           LP+DRIP LP
Sbjct: 181 LPYDRIPRLP 190

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BLAST of Chy4G068710 vs. ExPASy TrEMBL
Match: A0A0A0LJW4 (Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G895670 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 8.9e-94
Identity = 175/177 (98.87%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 178
           WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

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BLAST of Chy4G068710 vs. ExPASy TrEMBL
Match: A0A1S3CRS9 (Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.0e-93
Identity = 174/177 (98.31%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 178
           WELCGQPKIVVTCKNQHEMNKLKD+AENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of Chy4G068710 vs. ExPASy TrEMBL
Match: A0A1S3CQP0 (Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.3e-89
Identity = 173/190 (91.05%), Postives = 176/190 (92.63%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCA-------------HAATGLY 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCA              AATGLY
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAPKYFCMFFCQICEDAATGLY 120

Query: 121 AELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAG 178
           AELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKD+AENIGLPTFVVADAGRTQVSAG
Sbjct: 121 AELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAG 180

[Close]

BLAST of Chy4G068710 vs. ExPASy TrEMBL
Match: A0A6J1DTL2 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111024134 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 2.3e-89
Identity = 166/177 (93.79%), Postives = 174/177 (98.31%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           ML +S SSRKKQQVKPEKEWL+VSF+PENFIPGLVIGFI GLFLDLSKPSKGNAK+GYFL
Sbjct: 1   MLKSSNSSRKKQQVKPEKEWLSVSFRPENFIPGLVIGFIFGLFLDLSKPSKGNAKRGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQL+PASN+GDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLTPASNDGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 178
           WELCGQPKIVVTCKNQHEMNKLKD+A+NIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAADNIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

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BLAST of Chy4G068710 vs. NCBI nr
Match: KAA0038619.1 (peptidyl-tRNA hydrolase 2 [Cucumis melo var. makuwa] >TYK31218.1 peptidyl-tRNA hydrolase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 374 bits (961), Expect = 1.11e-130
Identity = 185/190 (97.37%), Postives = 189/190 (99.47%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 180
           WELCGQPKIVVTCKNQHEMNKLKD+AENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNAS 180

Query: 181 LPHDRIPWLP 190
           LP+DRIP LP
Sbjct: 181 LPYDRIPRLP 190

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BLAST of Chy4G068710 vs. NCBI nr
Match: XP_004136220.1 (peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis sativus] >KGN60321.1 hypothetical protein Csa_002634 [Cucumis sativus])

HSP 1 Score: 352 bits (903), Expect = 8.05e-122
Identity = 175/177 (98.87%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177
           WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of Chy4G068710 vs. NCBI nr
Match: XP_008466065.1 (PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 351 bits (900), Expect = 2.31e-121
Identity = 174/177 (98.31%), Postives = 177/177 (100.00%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177
           WELCGQPKIVVTCKNQHEMNKLKD+AENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177

[Close]

BLAST of Chy4G068710 vs. NCBI nr
Match: XP_011652615.1 (peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 343 bits (881), Expect = 1.69e-118
Identity = 173/177 (97.74%), Postives = 175/177 (98.87%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRK  QVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRK--QVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQ 120

Query: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 177
           WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG
Sbjct: 121 WELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPG 175

[Close]

BLAST of Chy4G068710 vs. NCBI nr
Match: XP_008466063.1 (PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 338 bits (867), Expect = 3.90e-116
Identity = 173/190 (91.05%), Postives = 176/190 (92.63%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           MLGASKSSRKKQQVKPEKEWL+VSFKPENFIPGLVIGFI+GLFLDLSKPSKGNAKKGYFL
Sbjct: 1   MLGASKSSRKKQQVKPEKEWLSVSFKPENFIPGLVIGFILGLFLDLSKPSKGNAKKGYFL 60

Query: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCA-------------HAATGLY 120
           PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCA              AATGLY
Sbjct: 61  PSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAPKYFCMFFCQICEDAATGLY 120

Query: 121 AELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAG 177
           AELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKD+AENIGLPTFVVADAGRTQVSAG
Sbjct: 121 AELMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDAAENIGLPTFVVADAGRTQVSAG 180

[Close]

BLAST of Chy4G068710 vs. TAIR 10
Match: AT4G32900.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 227.6 bits (579), Expect = 8.3e-60
Identity = 117/182 (64.29%), Postives = 145/182 (79.67%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           M  +S++S + Q ++ EK+ ++VSF+ EN IPG+VIGFIIG+ LDLS+      K+   L
Sbjct: 1   MWPSSRNSSQPQAMRQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLL 60

Query: 61  PSKYQQLSPASNNG-DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLR 120
            SK Q+ S    NG DQELK+VLVVRQDLKM +GKIASQCAHAATG+YAELMQS R LLR
Sbjct: 61  SSKVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLR 120

Query: 121 QWELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAIGPGNA 180
           +WE  GQPKIVVTCKNQ EMNK+ ++AE++GLPTFVVADAGRT+V+AGS+TVLA+GPG  
Sbjct: 121 RWEENGQPKIVVTCKNQQEMNKITEAAESVGLPTFVVADAGRTEVAAGSRTVLAVGPGPK 180

Query: 181 SL 182
            L
Sbjct: 181 EL 182

[Close]

BLAST of Chy4G068710 vs. TAIR 10
Match: AT4G32900.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 167.2 bits (422), Expect = 1.3e-41
Identity = 88/140 (62.86%), Postives = 107/140 (76.43%), Query Frame = 0


Query: 1   MLGASKSSRKKQQVKPEKEWLAVSFKPENFIPGLVIGFIIGLFLDLSKPSKGNAKKGYFL 60
           M  +S++S + Q ++ EK+ ++VSF+ EN IPG+VIGFIIG+ LDLS+      K+   L
Sbjct: 1   MWPSSRNSSQPQAMRQEKKSISVSFRAENLIPGVVIGFIIGMILDLSQQVTSPVKRSRLL 60

Query: 61  PSKYQQLSPASNNG-DQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLR 120
            SK Q+ S    NG DQELK+VLVVRQDLKM +GKIASQCAHAATG+YAELMQS R LLR
Sbjct: 61  SSKVQKQSSVPGNGKDQELKMVLVVRQDLKMRTGKIASQCAHAATGMYAELMQSDRYLLR 120

Query: 121 QWELCGQPKIVVTCKNQHEM 140
           +WE  GQPKIVVTCKNQ EM
Sbjct: 121 RWEENGQPKIVVTCKNQQEM 140

[Close]

BLAST of Chy4G068710 vs. TAIR 10
Match: AT3G03010.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.7e-25
Identity = 59/128 (46.09%), Postives = 83/128 (64.84%), Query Frame = 0


Query: 50  SKGNAKKGYFLPSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAE 109
           S GN K     P + ++L+    N     K+VLVVR DLKMG GKIA+QC+HA  GLY +
Sbjct: 42  SSGNKKTKSKEPLEIEKLADFRKN----FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKK 101

Query: 110 LMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSK 169
           L+Q     L +WE C QPK+VV  +++ EM  L++ A+ + LPT +  DAG+TQ++  S+
Sbjct: 102 LLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSR 161

Query: 170 TVLAI-GP 177
           TV+AI GP
Sbjct: 162 TVMAILGP 165

[Close]

BLAST of Chy4G068710 vs. TAIR 10
Match: AT3G03010.2 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.7e-25
Identity = 59/128 (46.09%), Postives = 83/128 (64.84%), Query Frame = 0


Query: 50  SKGNAKKGYFLPSKYQQLSPASNNGDQELKLVLVVRQDLKMGSGKIASQCAHAATGLYAE 109
           S GN K     P + ++L+    N     K+VLVVR DLKMG GKIA+QC+HA  GLY +
Sbjct: 42  SSGNKKTKSKEPLEIEKLADFRKN----FKMVLVVRNDLKMGKGKIAAQCSHATLGLYKK 101

Query: 110 LMQSHRNLLRQWELCGQPKIVVTCKNQHEMNKLKDSAENIGLPTFVVADAGRTQVSAGSK 169
           L+Q     L +WE C QPK+VV  +++ EM  L++ A+ + LPT +  DAG+TQ++  S+
Sbjct: 102 LLQRAPKALNRWEYCAQPKVVVKIESEEEMLVLQERAKTLKLPTHITIDAGKTQIAPNSR 161

Query: 170 TVLAI-GP 177
           TV+AI GP
Sbjct: 162 TVMAILGP 165

[Close]

BLAST of Chy4G068710 vs. TAIR 10
Match: AT5G16870.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.3e-24
Identity = 52/101 (51.49%), Postives = 74/101 (73.27%), Query Frame = 0


Query: 77  ELKLVLVVRQDLKMGSGKIASQCAHAATGLYAELMQSHRNLLRQWELCGQPKIVVTCKNQ 136
           + K+VLVVR DLKMG GKIA+QC+HA  GLY +L++     L  WE C QPK+VV  +++
Sbjct: 55  DFKMVLVVRNDLKMGKGKIAAQCSHATLGLYKKLVRRAPKALDCWEECAQPKVVVKIEDE 114

Query: 137 HEMNKLKDSAENIGLPTFVVADAGRTQVSAGSKTVLAI-GP 177
            EM +L++ A+++ LPT +  DAGRTQ++  S+TV+AI GP
Sbjct: 115 DEMLELQERAKSLKLPTHITIDAGRTQIAPNSRTVMAILGP 155

The following BLAST results are available for this feature:

BLAST of Chy4G068710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9Y3E5	3.4e-26	43.05	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Homo sapiens OX=9606 GN=PTRH2 PE=1 S...	[more]
Q3ZBL5	2.9e-25	49.52	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Bos taurus OX=9913 GN=PTRH2 PE=2 SV=...	[more]
Q8R2Y8	7.1e-24	47.62	Peptidyl-tRNA hydrolase 2, mitochondrial OS=Mus musculus OX=10090 GN=Ptrh2 PE=1 ...	[more]
O97067	2.3e-22	39.05	Probable peptidyl-tRNA hydrolase 2 OS=Drosophila melanogaster OX=7227 GN=CG1307 ...	[more]
O13830	8.7e-22	35.14	Probable peptidyl-tRNA hydrolase 2 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]

BLAST of Chy4G068710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E5Y4	2.8e-100	97.37	Aminoacyl-tRNA hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0LJW4	8.9e-94	98.87	Aminoacyl-tRNA hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G895670 PE=4 SV=1	[more]
A0A1S3CRS9	2.0e-93	98.31	Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1	[more]
A0A1S3CQP0	1.3e-89	91.05	Aminoacyl-tRNA hydrolase OS=Cucumis melo OX=3656 GN=LOC103503602 PE=4 SV=1	[more]
A0A6J1DTL2	2.3e-89	93.79	Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111024134 PE=4 SV=...	[more]

BLAST of Chy4G068710 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0038619.1	1.11e-130	97.37	peptidyl-tRNA hydrolase 2 [Cucumis melo var. makuwa] >TYK31218.1 peptidyl-tRNA h...	[more]
XP_004136220.1	8.05e-122	98.87	peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis sativus] >KGN60321....	[more]
XP_008466065.1	2.31e-121	98.31	PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis melo]	[more]
XP_011652615.1	1.69e-118	97.74	peptidyl-tRNA hydrolase 2, mitochondrial isoform X2 [Cucumis sativus]	[more]
XP_008466063.1	3.90e-116	91.05	PREDICTED: peptidyl-tRNA hydrolase 2, mitochondrial isoform X1 [Cucumis melo]	[more]

BLAST of Chy4G068710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32900.2	8.3e-60	64.29	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT4G32900.1	1.3e-41	62.86	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.1	1.7e-25	46.09	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT3G03010.2	1.7e-25	46.09	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]
AT5G16870.1	7.3e-24	51.49	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002833	Peptidyl-tRNA hydrolase, PTH2	TIGRFAM	TIGR00283	TIGR00283	coord: 78..178 e-value: 2.5E-32 score: 109.6
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 78..180 e-value: 1.2E-37 score: 128.8
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PANTHER	PTHR12649	PEPTIDYL-TRNA HYDROLASE 2	coord: 5..180
IPR002833	Peptidyl-tRNA hydrolase, PTH2	CDD	cd02430	PTH2	coord: 79..181 e-value: 2.39438E-50 score: 156.53
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 73..185 e-value: 4.8E-44 score: 151.1
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 77..182
None	No IPR available	PANTHER	PTHR12649:SF11	PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL	coord: 5..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G068710.1	Chy4G068710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity