Chy4G067600 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G067600
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	BOI-related E3 ubiquitin-protein ligase 1
Location	chrH04: 463976 .. 465323 (-)
RNA-Seq Expression	Chy4G067600
Synteny	Chy4G067600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTTCAGGCTCAATATCCTTCTAATGTTTTGTTATTGAACAGGTGTGTGTATTTACACATTTTCTACTTACTCTTCTTGTTTTGGTTGTTAAGTTTTTCCAAATCTTTTAATGGGTTTTTTGGAATTTTTGGTTATGGTTTTAGGAATGGTCAAGAAGGGCATGATTATTCATTACAGCCACAACCAGGGGGTTTTCTTGACCAAACTCATATGGTGTTCAACAATAATATAGGTGAGTTTTCTTGTACATTGTTTTTTTTTTTTTTTTTTTGGATGGATGGATTTCTTTAGGTTTTTGTGTTGTTCTATTCTAGTTATGAAACATCAGACAAAAGTATTGAGATAAATTTGAGTTGTGAGAAGAGGCTGTACCTTTTAGAAATCAATTAATCAATTGAATTTCTGATGAAACCAGGAAGCAATTCTCGAAAGAGAGGAAGAGAAATCTCTACTGCTACTACAACCATCGCGACTCCAATCAATCTATCTCCATTACAGCACCAGCCTTGTCCTCCTCTTCTTGTTGACCTTGCTAAGCTCCATAACCACCAAAACAATGTCGTCTCCACCGGCCTCCGCCTCTCCTCCGGTGATCATCAACAGAACCAACACCAACACCAACAACATAATCAAAACCACAATCTCGTTCGTCAATCATCATCCGCTTTCTTACCCCTACTAACAGAGGATTTCGCCTCAAATTTCAAACGGCATCAAGAAGAAATAGACCAATTCCTTCAAGCACAGGTGCTAATTCACTCCCTAACACACAACGAGTTTCATCTTCCGTCTTGATTTTAAATTTCTGAAGGAGTTCCGTCCGTCACAGGAAGAGCAACTACGGCGCACATTAGCAGAGAAAAGACAGAGACACTACCGTGCGCTCCTCGATGCGGTGGAGGAATCGGTAGCCAGAAGGTTACGAGAGAGGGAGGCGGAGGTGGAGAAAGCCACGCGCCGGAATGCCGAATTAGAAGCACGCGCCGCCCAGCTAAGTGTCGAGGCACAGGTCTGGCAAGCAAAGGCTAGGGCACAGGAAGCCACCGCCGTATCCCTACAAGCACAACTGCAACAGGCGATGTCCGGCGGTGGATGCGACGGAGGAGATGGCGGAACCGTGGGCGTGGAAGGACAAGCCGATGACGCAGAGTCGGCTCATATTGACCCGGAACGAGTCGTGATTTCGGGACCGAGTTGCAAAGCGTGTAGAAAACGGTTGGCTTCGATGCTGATTTTGCCGTGCCGTCATCTCTGCCTCTGTACAGACTGTGACCAAGTGGCTCAGACTTGCCCGTTATGCCATTCCAAACGAAGTTCCAGTGTAGAGGTCTATCTCTCTTAG

mRNA sequence

ATGGCTGTTCAGGCTCAATATCCTTCTAATGTTTTGTTATTGAACAGGAATGGTCAAGAAGGGCATGATTATTCATTACAGCCACAACCAGGGGGTTTTCTTGACCAAACTCATATGGTGTTCAACAATAATATAGGAAGCAATTCTCGAAAGAGAGGAAGAGAAATCTCTACTGCTACTACAACCATCGCGACTCCAATCAATCTATCTCCATTACAGCACCAGCCTTGTCCTCCTCTTCTTGTTGACCTTGCTAAGCTCCATAACCACCAAAACAATGTCGTCTCCACCGGCCTCCGCCTCTCCTCCGGTGATCATCAACAGAACCAACACCAACACCAACAACATAATCAAAACCACAATCTCGTTCGTCAATCATCATCCGCTTTCTTACCCCTACTAACAGAGGATTTCGCCTCAAATTTCAAACGGCATCAAGAAGAAATAGACCAATTCCTTCAAGCACAGGAAGAGCAACTACGGCGCACATTAGCAGAGAAAAGACAGAGACACTACCGTGCGCTCCTCGATGCGGTGGAGGAATCGGTAGCCAGAAGGTTACGAGAGAGGGAGGCGGAGGTGGAGAAAGCCACGCGCCGGAATGCCGAATTAGAAGCACGCGCCGCCCAGCTAAGTGTCGAGGCACAGGTCTGGCAAGCAAAGGCTAGGGCACAGGAAGCCACCGCCGTATCCCTACAAGCACAACTGCAACAGGCGATGTCCGGCGGTGGATGCGACGGAGGAGATGGCGGAACCGTGGGCGTGGAAGGACAAGCCGATGACGCAGAGTCGGCTCATATTGACCCGGAACGAGTCGTGATTTCGGGACCGAGTTGCAAAGCGTGTAGAAAACGGTTGGCTTCGATGCTGATTTTGCCGTGCCGTCATCTCTGCCTCTGTACAGACTGTGACCAAGTGGCTCAGACTTGCCCGTTATGCCATTCCAAACGAAGTTCCAGTGTAGAGGTCTATCTCTCTTAG

Coding sequence (CDS)

ATGGCTGTTCAGGCTCAATATCCTTCTAATGTTTTGTTATTGAACAGGAATGGTCAAGAAGGGCATGATTATTCATTACAGCCACAACCAGGGGGTTTTCTTGACCAAACTCATATGGTGTTCAACAATAATATAGGAAGCAATTCTCGAAAGAGAGGAAGAGAAATCTCTACTGCTACTACAACCATCGCGACTCCAATCAATCTATCTCCATTACAGCACCAGCCTTGTCCTCCTCTTCTTGTTGACCTTGCTAAGCTCCATAACCACCAAAACAATGTCGTCTCCACCGGCCTCCGCCTCTCCTCCGGTGATCATCAACAGAACCAACACCAACACCAACAACATAATCAAAACCACAATCTCGTTCGTCAATCATCATCCGCTTTCTTACCCCTACTAACAGAGGATTTCGCCTCAAATTTCAAACGGCATCAAGAAGAAATAGACCAATTCCTTCAAGCACAGGAAGAGCAACTACGGCGCACATTAGCAGAGAAAAGACAGAGACACTACCGTGCGCTCCTCGATGCGGTGGAGGAATCGGTAGCCAGAAGGTTACGAGAGAGGGAGGCGGAGGTGGAGAAAGCCACGCGCCGGAATGCCGAATTAGAAGCACGCGCCGCCCAGCTAAGTGTCGAGGCACAGGTCTGGCAAGCAAAGGCTAGGGCACAGGAAGCCACCGCCGTATCCCTACAAGCACAACTGCAACAGGCGATGTCCGGCGGTGGATGCGACGGAGGAGATGGCGGAACCGTGGGCGTGGAAGGACAAGCCGATGACGCAGAGTCGGCTCATATTGACCCGGAACGAGTCGTGATTTCGGGACCGAGTTGCAAAGCGTGTAGAAAACGGTTGGCTTCGATGCTGATTTTGCCGTGCCGTCATCTCTGCCTCTGTACAGACTGTGACCAAGTGGCTCAGACTTGCCCGTTATGCCATTCCAAACGAAGTTCCAGTGTAGAGGTCTATCTCTCTTAG

Protein sequence

MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTATTTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQHQHQQHNQNHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS*

Homology

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BLAST of Chy4G067600 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.0e-18
Identity = 96/337 (28.49%), Postives = 162/337 (48.07%), Query Frame = 0


Query: 24  YSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREIST------ATTTIATPI-NLSP---LQ 83
           + L P      +  H V  + +  N++ R   + T        T++  PI N+SP   L 
Sbjct: 6   HHLNPLFSSNREMIHPVEASGVVYNTQMRYGTVPTFNPTVECQTSLFNPIYNISPVDRLV 65

Query: 84  HQPCPPLL--VDLAKLHNHQNNVVSTGLRLSSGDHQQNQHQHQQHNQNHNLVRQSSSAFL 143
           HQ   P +  VD +   N  NNV    LR  S   +  +     +   +  ++++ +  L
Sbjct: 66  HQSMKPTIQSVDSSLTFNSDNNV--DFLRPVSSRKRSREESVVLNPSAYMQIQKNPTDPL 125

Query: 144 PLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLRERE 203
             L +D +SN ++H  +ID+ +    E++R  + EKR+   R +++AVE+ + + LR ++
Sbjct: 126 MFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKD 185

Query: 204 AEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSG--------- 263
            E+    + N  LE +   L VE Q+W+  A++ EAT  +L++ LQQ ++          
Sbjct: 186 DEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEP 245

Query: 264 --------GGCDGGDGGTVGVE-----GQADDAESAHIDPERVVISGPSCKACRKRLASM 323
                     C   D G    E     G+A D +       RV +S   C++C K  AS+
Sbjct: 246 PTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKKMC----RVGMS--MCRSCGKGEASV 305

Query: 324 LILPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           L+LPCRH+CLC+ C     TCP+C S +++S+ V LS
Sbjct: 306 LLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

[Close]

BLAST of Chy4G067600 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.8e-18
Identity = 72/211 (34.12%), Postives = 119/211 (56.40%), Query Frame = 0


Query: 124 RQSSSAFLP--LLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEE 183
           ++ + AF P  L+  +  S  ++   EID+F+  Q E LR  L  +++   R L  AV+ 
Sbjct: 86  KRRAIAFSPASLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQN 145

Query: 184 SVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMS 243
           ++ ++L+ ++ E+ +  + N  L+ R   L VE Q+W+  A+  EATA +L++ L+Q ++
Sbjct: 146 AILKKLKAKDEEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLA 205

Query: 244 GGGCDGGDGGTVGVEGQADDAESAHIDPE----RVVISGPSCKACRKRLASMLILPCRHL 303
               D  D     +  +ADDAES+    +      V++G  CK C +  AS+L+LPCRHL
Sbjct: 206 --QVDDLDAFRRPLVEEADDAESSCGSCDGGDVTAVVNG-GCKRCGELTASVLVLPCRHL 265

Query: 304 CLCTDCDQVA--QTCPLCHSKRSSSVEVYLS 327
           CLCT C   A  +TCP+C    ++SV V +S
Sbjct: 266 CLCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of Chy4G067600 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.6e-18
Identity = 72/222 (32.43%), Postives = 113/222 (50.90%), Query Frame = 0


Query: 137 DFASNFKRHQEEIDQFLQA---QEEQLRRTLAEKRQRHYRALLDAVEESVARRLREREAE 196
           D +S+  + Q EID+F+     Q E+++  + EKR+R  R +++A+E+ + +RLR +E E
Sbjct: 144 DISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEE 203

Query: 197 VEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMS------------ 256
            E+  + N  LE R   LS+E Q+W+  A+  EATA  L+  L+  ++            
Sbjct: 204 RERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLE 263

Query: 257 -------------GGGCDGGDGGT----VGVEGQADDAESAHIDPERVVISGPSCKACRK 316
                        G  C GG   T    VG+E +A D   A     R+      C+ C +
Sbjct: 264 KNMNEEDDAESCCGSSCGGGGEETVRRRVGLEREAQD--KAERRRRRM------CRNCGE 323

Query: 317 RLASMLILPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
             + +L+LPCRHLCLC  C     TCP+C S +++SV V +S
Sbjct: 324 EESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVHVNMS 357

[Close]

BLAST of Chy4G067600 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-15
Identity = 77/231 (33.33%), Postives = 121/231 (52.38%), Query Frame = 0


Query: 108 QNQHQHQQHNQNHNLVRQS---SSAFLPLLTEDFASNFKRHQE-EIDQFLQAQEEQLRRT 167
           + Q   Q  + N  + R+S    S+   L+  +  S  +  Q+ EID+F+  Q E+LR  
Sbjct: 78  KRQRDFQFSDSNAPVKRRSVAFDSSSPSLINVELVSQIQNQQQSEIDRFVAQQTEKLRIE 137

Query: 168 LAEKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKAR 227
           +  ++Q   R L  AV+  +A++L+E++ E+ +    N  L+ R   L VE Q+W+  A+
Sbjct: 138 IEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQ 197

Query: 228 AQEATAVSLQAQLQQAMSGGGCDGGDGGTVGVEGQADDAESA------HIDPERVVISGP 287
             EA A +L+  L Q ++    +     +  VE   DDAES+          E V   G 
Sbjct: 198 TNEANANTLRTNLDQVLA--QLETFPTASAVVE---DDAESSCGSCCGDGGGEAVTAVGG 257

Query: 288 SCKACRKRLASMLILPCRHLCLCTDC--DQVAQTCPLCHSKRSSSVEVYLS 327
            CK C +R AS+L+LPCRHLCLCT C    + +TCP+C    ++SV V +S
Sbjct: 258 GCKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of Chy4G067600 vs. ExPASy Swiss-Prot
Match: Q6UWE0 (E3 ubiquitin-protein ligase LRSAM1 OS=Homo sapiens OX=9606 GN=LRSAM1 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.3e-04
Identity = 20/58 (34.48%), Postives = 29/58 (50.00%), Query Frame = 0


Query: 269 PERVVISGPSCKACRKRLASMLILPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           P  + +    C  C +R A M+ L C H+C C  C Q  +TCPLC    +  + +Y S
Sbjct: 665 PAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRIYHS 722

[Close]

BLAST of Chy4G067600 vs. ExPASy TrEMBL
Match: A0A0A0LEI4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G911260 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.6e-170
Identity = 322/328 (98.17%), Postives = 322/328 (98.17%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN--QHQHQQHNQ 120
           TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN  Q Q QQHNQ
Sbjct: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQQQQQQQHNQ 120

Query: 121 NHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180
           NHNLVR SSSAFLPLLTEDFASNFKR QEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA
Sbjct: 121 NHNLVRPSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180

Query: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240
           VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ
Sbjct: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240

Query: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300
           AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC
Sbjct: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300

Query: 301 LCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           LCTDCDQVAQTCPLCHSKRSSSVEVYLS
Sbjct: 301 LCTDCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Chy4G067600 vs. ExPASy TrEMBL
Match: A0A1S3CRF0 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC103503495 PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.4e-168
Identity = 318/328 (96.95%), Postives = 320/328 (97.56%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHM+FNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMMFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN--QHQHQQHNQ 120
           TT+ATPINLSPLQHQP PPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN  Q Q QQHN 
Sbjct: 61  TTVATPINLSPLQHQPHPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQQQQQQQHNH 120

Query: 121 NHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180
           NHNLVRQSSSAFLPLLTEDFASNFKR QEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA
Sbjct: 121 NHNLVRQSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180

Query: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240
           VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ
Sbjct: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240

Query: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300
           AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC
Sbjct: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300

Query: 301 LCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           LC DCDQVAQTCPLCHSKRSSSVEVYLS
Sbjct: 301 LCADCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Chy4G067600 vs. ExPASy TrEMBL
Match: A0A6J1IE25 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471820 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 5.8e-146
Identity = 286/335 (85.37%), Postives = 305/335 (91.04%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYSLQ QPGGFLDQTHMVFNNNIG+NSRKRGRE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNIGNNSRKRGREMATST 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN---------QH 120
            T ATPI+LS +Q QP PPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQN         Q 
Sbjct: 61  ATFATPIDLSAIQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQQQQQQQ 120

Query: 121 QHQQHNQNHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRH 180
           Q QQHN NHNLVRQSSSAF+ LLT+DFASNFKR Q+EIDQFLQAQEEQLR TLA+KRQRH
Sbjct: 121 QQQQHNHNHNLVRQSSSAFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRH 180

Query: 181 YRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVS 240
           YRALLDAVEESVARRLRE+EAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATA++
Sbjct: 181 YRALLDAVEESVARRLREKEAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATALA 240

Query: 241 LQAQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLI 300
           LQAQL+QAMS GGCDGGDGGT G EGQ DDAESA+IDP+RV I GPSCKACRKRLAS+L+
Sbjct: 241 LQAQLKQAMS-GGCDGGDGGTAGAEGQTDDAESAYIDPDRVRILGPSCKACRKRLASVLL 300

Query: 301 LPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           LPCRHLCLCT CDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 LPCRHLCLCTVCDQVAQTCPLCPSKRSSSVEVYLS 333

[Close]

BLAST of Chy4G067600 vs. ExPASy TrEMBL
Match: A0A6J1EXW3 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439606 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.9e-145
Identity = 285/330 (86.36%), Postives = 301/330 (91.21%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYS Q QPGGFLDQTHMVFNNNIG+NSRKRGRE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIGNNSRKRGREMATST 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN----QHQHQQH 120
            T ATPI+LS +Q QP PPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQN    Q Q QQ 
Sbjct: 61  ATFATPIDLSAIQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQQQQQQP 120

Query: 121 NQNHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALL 180
           N NHNLV QSSS F+ LLT+DFASNFKR Q+EIDQFLQAQEEQLR TLA+KRQRHYRALL
Sbjct: 121 NHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRHYRALL 180

Query: 181 DAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQL 240
           DAVEESVARRLRERE+EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAV+LQAQL
Sbjct: 181 DAVEESVARRLRERESEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQAQL 240

Query: 241 QQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRH 300
           QQAMS GGCDGGDGGT G EGQ DDAESAHIDP+RV I GPSCKACRKRLAS+L+LPCRH
Sbjct: 241 QQAMS-GGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASVLLLPCRH 300

Query: 301 LCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           LCLCTDCDQVA TCPLC SKRSSSVEVYLS
Sbjct: 301 LCLCTDCDQVATTCPLCPSKRSSSVEVYLS 328

[Close]

BLAST of Chy4G067600 vs. ExPASy TrEMBL
Match: A0A6J1E527 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN=LOC111026119 PE=4 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.1e-142
Identity = 289/333 (86.79%), Postives = 306/333 (91.89%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVF NNIG+NSRKRGREISTA 
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVF-NNIGNNSRKRGREISTA- 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN---QHQH-QQH 120
           +TI  PINLS LQ QP PPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN   QHQH   H
Sbjct: 61  STIGAPINLSALQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQSQQHQHNHNH 120

Query: 121 NQNHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALL 180
           N NHNLVRQ SS FL LLT++FASNFKR Q+EIDQFLQAQEEQLRRTLAEKRQRHYRALL
Sbjct: 121 NHNHNLVRQ-SSPFLSLLTDNFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRHYRALL 180

Query: 181 DAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQL 240
           DAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQL
Sbjct: 181 DAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQL 240

Query: 241 QQAMS--GGGCDGGDGGTV-GVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILP 300
           QQAMS  GGG DG DGG + G+EGQADDAESA++DP+RVV+ GPSCKACRKR+AS+++LP
Sbjct: 241 QQAMSGGGGGGDGVDGGNLAGIEGQADDAESAYVDPDRVVMLGPSCKACRKRVASVVLLP 300

Query: 301 CRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           CRHLCLCTDCDQV QTCPLC S+R+SSVEVYLS
Sbjct: 301 CRHLCLCTDCDQVVQTCPLCLSQRNSSVEVYLS 329

[Close]

BLAST of Chy4G067600 vs. NCBI nr
Match: XP_004148373.2 (BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN60440.1 hypothetical protein Csa_002624 [Cucumis sativus])

HSP 1 Score: 606 bits (1562), Expect = 9.44e-218
Identity = 322/328 (98.17%), Postives = 322/328 (98.17%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQHQHQQ--HNQ 120
           TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ Q QQ  HNQ
Sbjct: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQQQQQQQHNQ 120

Query: 121 NHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180
           NHNLVR SSSAFLPLLTEDFASNFKR QEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA
Sbjct: 121 NHNLVRPSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180

Query: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240
           VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ
Sbjct: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240

Query: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300
           AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC
Sbjct: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300

Query: 301 LCTDCDQVAQTCPLCHSKRSSSVEVYLS 326
           LCTDCDQVAQTCPLCHSKRSSSVEVYLS
Sbjct: 301 LCTDCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Chy4G067600 vs. NCBI nr
Match: XP_008465916.1 (PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo])

HSP 1 Score: 598 bits (1542), Expect = 1.05e-214
Identity = 318/328 (96.95%), Postives = 320/328 (97.56%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHM+FNNNIGSNSRKRGREISTAT
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMMFNNNIGSNSRKRGREISTAT 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQHQHQQ--HNQ 120
           TT+ATPINLSPLQHQP PPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ Q QQ  HN 
Sbjct: 61  TTVATPINLSPLQHQPHPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQQQQQQQQHNH 120

Query: 121 NHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180
           NHNLVRQSSSAFLPLLTEDFASNFKR QEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA
Sbjct: 121 NHNLVRQSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180

Query: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240
           VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ
Sbjct: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240

Query: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300
           AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC
Sbjct: 241 AMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLC 300

Query: 301 LCTDCDQVAQTCPLCHSKRSSSVEVYLS 326
           LC DCDQVAQTCPLCHSKRSSSVEVYLS
Sbjct: 301 LCADCDQVAQTCPLCHSKRSSSVEVYLS 328

[Close]

BLAST of Chy4G067600 vs. NCBI nr
Match: XP_038888350.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida])

HSP 1 Score: 541 bits (1394), Expect = 3.72e-192
Identity = 304/335 (90.75%), Postives = 309/335 (92.24%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGF-LDQTHMVFNNNIGSNSRKRGREISTA 60
           MAVQAQYPSNVLLLNRNGQEGHDYSLQ QPGGF LDQTHMVFNNNIGSNSRKRGREI+TA
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQQQPGGFILDQTHMVFNNNIGSNSRKRGREIATA 60

Query: 61  TTTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN-------QHQ 120
           T     PINLS LQ QP  PLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN       Q Q
Sbjct: 61  T-----PINLSALQ-QP-RPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNHPPQQQQQQQ 120

Query: 121 HQQHNQNHNLVRQSSS-AFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRH 180
            QQHN NHNLVRQSSS AFLPLLT+DFASNFKR Q+EIDQFLQAQEEQLRRTLAEKRQRH
Sbjct: 121 QQQHNHNHNLVRQSSSSAFLPLLTDDFASNFKRQQDEIDQFLQAQEEQLRRTLAEKRQRH 180

Query: 181 YRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVS 240
           YRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKA+AQEATAVS
Sbjct: 181 YRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKAKAQEATAVS 240

Query: 241 LQAQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLI 300
           LQAQLQQAMSGGGCDGG+GGT GVEGQADDAESAHIDPERV ISGPSCKACRKRLASMLI
Sbjct: 241 LQAQLQQAMSGGGCDGGEGGTAGVEGQADDAESAHIDPERVAISGPSCKACRKRLASMLI 300

Query: 301 LPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 326
           LPCRHLCLCTDCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 LPCRHLCLCTDCDQVAQTCPLCLSKRSSSVEVYLS 328

[Close]

BLAST of Chy4G067600 vs. NCBI nr
Match: KAG6571752.1 (putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 525 bits (1353), Expect = 7.02e-186
Identity = 287/332 (86.45%), Postives = 302/332 (90.96%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYS Q QPGGFLDQTHMVFNNNIG+NSRKRGRE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIGNNSRKRGREMATST 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ------HQHQ 120
            T ATPI+LS +Q QP PPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQNQ       Q Q
Sbjct: 61  ATFATPIDLSAIQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQRKQQQQ 120

Query: 121 QHNQNHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRA 180
           Q N NHNLV QSSS F+ LLT+DFASNFKR Q+EIDQFLQAQEEQLR TLA+KRQRHYRA
Sbjct: 121 QPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRHYRA 180

Query: 181 LLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQA 240
           LLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAV+LQA
Sbjct: 181 LLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQA 240

Query: 241 QLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLILPC 300
           QLQQAMSGG CDGGDGGT G EGQ DDAESAHIDP+RV I GPSCKACRKRLAS+L+LPC
Sbjct: 241 QLQQAMSGG-CDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASVLLLPC 300

Query: 301 RHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 326
           RHLCLCTDCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 RHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 330

[Close]

BLAST of Chy4G067600 vs. NCBI nr
Match: XP_022973264.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima])

HSP 1 Score: 525 bits (1352), Expect = 1.11e-185
Identity = 286/335 (85.37%), Postives = 305/335 (91.04%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQAQYPSNVLLLNRNGQE HDYSLQ QPGGFLDQTHMVFNNNIG+NSRKRGRE++T+T
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNIGNNSRKRGREMATST 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQ---------H 120
            T ATPI+LS +Q QP PPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQNQ          
Sbjct: 61  ATFATPIDLSAIQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQQQQQQQ 120

Query: 121 QHQQHNQNHNLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRH 180
           Q QQHN NHNLVRQSSSAF+ LLT+DFASNFKR Q+EIDQFLQAQEEQLR TLA+KRQRH
Sbjct: 121 QQQQHNHNHNLVRQSSSAFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKRQRH 180

Query: 181 YRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVS 240
           YRALLDAVEESVARRLRE+EAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATA++
Sbjct: 181 YRALLDAVEESVARRLREKEAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATALA 240

Query: 241 LQAQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLI 300
           LQAQL+QAMSGG CDGGDGGT G EGQ DDAESA+IDP+RV I GPSCKACRKRLAS+L+
Sbjct: 241 LQAQLKQAMSGG-CDGGDGGTAGAEGQTDDAESAYIDPDRVRILGPSCKACRKRLASVLL 300

Query: 301 LPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 326
           LPCRHLCLCT CDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 LPCRHLCLCTVCDQVAQTCPLCPSKRSSSVEVYLS 333

[Close]

BLAST of Chy4G067600 vs. TAIR 10
Match: AT1G32740.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.2e-62
Identity = 164/340 (48.24%), Postives = 214/340 (62.94%), Query Frame = 0


Query: 1   MAVQAQ-YPSNVLLLN-RNGQEGH--DYSLQPQPGG-FLDQTHMVFNNNIGSNSRKRGRE 60
           MAVQAQ + SN+L LN RNG+E    +++LQ Q  G FLDQT+M+FNN   SN RKR RE
Sbjct: 1   MAVQAQHHSSNLLFLNKRNGKEKEHSNFTLQSQAAGDFLDQTNMLFNNG-SSNQRKRRRE 60

Query: 61  ISTATTTIATPINLSPLQHQPCPPLLVDLAKLHNHQ---NNVVSTGLRLSSGDHQQNQHQ 120
            +           L P+Q    P  ++DL+ LHN+    +N+V TGLRL SG+ Q  +  
Sbjct: 61  TNNH--------QLLPMQSHQFPQ-VIDLSLLHNYNHPPSNMVHTGLRLFSGEDQAQKIS 120

Query: 121 HQQHNQNHNLVRQSSSAFLPLLTED-FASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRH 180
           H                    L+ED FA++  R  EE+D+FL AQ E+LRRTLAEKR+ H
Sbjct: 121 H--------------------LSEDVFAAHINRQSEELDEFLHAQAEELRRTLAEKRKMH 180

Query: 181 YRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVS 240
           Y+ALL AVEES+ R+LRE+E E+E+ATRR+ EL AR +QL  E QVWQ +A+A E  A S
Sbjct: 181 YKALLGAVEESLVRKLREKEVEIERATRRHNELVARDSQLRAEVQVWQERAKAHEDAAAS 240

Query: 241 LQAQLQQAMS--GGGCDGGDGGTVGVEG-------QADDAESAHIDPERVVISGPSCKAC 300
           LQ+QLQQA++   GGC          EG         DDAES ++DPERV    P+CKAC
Sbjct: 241 LQSQLQQAVNQCAGGCVSAQDSRAAEEGLLCTTISGVDDAESVYVDPERV--KRPNCKAC 300

Query: 301 RKRLASMLILPCRHLCLCTDCDQVAQTCPLCHSKRSSSVE 323
           R+R A++++LPCRHL +C  CD+ A  CPLC + R+SSVE
Sbjct: 301 REREATVVVLPCRHLSICPGCDRTALACPLCLTLRNSSVE 308

[Close]

BLAST of Chy4G067600 vs. TAIR 10
Match: AT5G47050.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 219.2 bits (557), Expect = 5.0e-57
Identity = 156/335 (46.57%), Postives = 205/335 (61.19%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MAVQA +  + L    NGQEG+D   QP    F    H +   N G ++RKR RE+S   
Sbjct: 1   MAVQAHHHPSTLFFLNNGQEGND---QPHKSQF----HSI---NAGVDTRKRAREVS--- 60

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQN-NVVSTGLRLSSGDHQQNQHQHQQHNQN 120
           + I   I  +P+   P  P  V    +   QN NVVSTGLRLS    +Q+Q+Q Q+    
Sbjct: 61  SVIDLDITAAPMNPPPQTPPQV----IGRRQNPNVVSTGLRLS---REQSQNQEQR---- 120

Query: 121 HNLVRQSSSAFLPL-LTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDA 180
                     FL   +T D A   K   +E+++FLQ Q EQL+R LAE  +R+YR LL  
Sbjct: 121 ----------FLSFPITGDVAGEIKSQTDELNRFLQIQGEQLKRMLAENSERNYRELLRT 180

Query: 181 VEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQ 240
            EESV RRLRE+EAE+EKATRR+ ELEARA Q+  EA+ WQ +A A+EA A SLQAQL Q
Sbjct: 181 TEESVRRRLREKEAEIEKATRRHVELEARATQIETEARAWQMRAAAREAEATSLQAQLHQ 240

Query: 241 AM---SGGG----CDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLASMLI 300
           A+    GGG     +   G   GV+ +A+DAESA++DP+RV + GP C+ CR+R A++L 
Sbjct: 241 AVVVAHGGGVITTVEPQSGSVDGVD-EAEDAESAYVDPDRVEMIGPGCRICRRRSATVLA 300

Query: 301 LPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 327
           LPCRHL +CT+CD   + CPLC S ++SSVEV+ S
Sbjct: 301 LPCRHLVMCTECDGSVRICPLCLSTKNSSVEVFYS 300

[Close]

BLAST of Chy4G067600 vs. TAIR 10
Match: AT4G17680.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 209.5 bits (532), Expect = 4.0e-54
Identity = 141/328 (42.99%), Postives = 192/328 (58.54%), Query Frame = 0


Query: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNIGSNSRKRGREISTAT 60
           MA+QAQ+PS     N NGQE  D SLQPQ   F + T        G +SRKR RE+ ++ 
Sbjct: 18  MAIQAQHPSRFFFNNSNGQEASDCSLQPQDTPFTNFT------KAGVDSRKRSREVYSSA 77

Query: 61  TTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQHQHQQHNQNH 120
                P         P P  ++D+ +L     NVVSTGLRL    H Q+Q+Q Q      
Sbjct: 78  LMNPPP---------PKPSQVIDITELLQKTPNVVSTGLRLF---HDQSQNQQQ------ 137

Query: 121 NLVRQSSSAFLPLLTEDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVE 180
                    F   L  D     KR ++E+D+F+Q Q E+LRRTLA+ R+R Y  LL A E
Sbjct: 138 ---------FFSSLPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRYVELLCAAE 197

Query: 181 ESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAM 240
           E V R+LR++EAE+EKATRR+AELEAR A +  EA+ WQ +A  +EA   SL A LQQA+
Sbjct: 198 EIVGRKLRKKEAELEKATRRHAELEARVAHIVEEARNWQLRAATREAEVSSLHAHLQQAI 257

Query: 241 SGGGCDGGDGGTVGVEG----QADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRH 300
           +          T G +G    +A+DAES ++DPER+ + GPSC+ CR++ A+++ LPC+H
Sbjct: 258 ANRLDTAAKQSTFGEDGGDAEEAEDAESVYVDPERIELIGPSCRICRRKSATVMALPCQH 312

Query: 301 LCLCTDCDQVA-QTCPLCHSKRSSSVEV 324
           L LC  CD  A + CP+C + ++S VEV
Sbjct: 318 LILCNGCDVGAVRVCPICLAVKTSGVEV 312

[Close]

BLAST of Chy4G067600 vs. TAIR 10
Match: AT4G17680.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.4e-43
Identity = 113/253 (44.66%), Postives = 157/253 (62.06%), Query Frame = 0


Query: 76  PCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQNQHQHQQHNQNHNLVRQSSSAFLPLLT 135
           P P  ++D+ +L     NVVSTGLRL    H Q+Q+Q Q               F   L 
Sbjct: 6   PKPSQVIDITELLQKTPNVVSTGLRLF---HDQSQNQQQ---------------FFSSLP 65

Query: 136 EDFASNFKRHQEEIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLREREAEVE 195
            D     KR ++E+D+F+Q Q E+LRRTLA+ R+R Y  LL A EE V R+LR++EAE+E
Sbjct: 66  GDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRYVELLCAAEEIVGRKLRKKEAELE 125

Query: 196 KATRRNAELEARAAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSGGGCDGGDGGTVGV 255
           KATRR+AELEAR A +  EA+ WQ +A  +EA   SL A LQQA++          T G 
Sbjct: 126 KATRRHAELEARVAHIVEEARNWQLRAATREAEVSSLHAHLQQAIANRLDTAAKQSTFGE 185

Query: 256 EG----QADDAESAHIDPERVVISGPSCKACRKRLASMLILPCRHLCLCTDCDQVA-QTC 315
           +G    +A+DAES ++DPER+ + GPSC+ CR++ A+++ LPC+HL LC  CD  A + C
Sbjct: 186 DGGDAEEAEDAESVYVDPERIELIGPSCRICRRKSATVMALPCQHLILCNGCDVGAVRVC 240

Query: 316 PLCHSKRSSSVEV 324
           P+C + ++S VEV
Sbjct: 246 PICLAVKTSGVEV 240

[Close]

BLAST of Chy4G067600 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 118.6 bits (296), Expect = 9.2e-27
Identity = 85/248 (34.27%), Postives = 134/248 (54.03%), Query Frame = 0


Query: 91  QNNVVSTGLRLSSGDHQQNQHQHQQHNQNHNLVRQSSSAFLPL---LTEDFASNFKRHQE 150
           + N+VSTGLRLS  D         +HN +      S  A  P+   L +    +  R ++
Sbjct: 100 KENLVSTGLRLSYDD--------DEHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKD 159

Query: 151 EIDQFLQAQEEQLRRTLAEKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEAR 210
           E DQF++ Q  Q+ + + + +QRH  + L  +E+ V+++L+E++ E+    ++N EL  R
Sbjct: 160 EFDQFIKIQAAQMAKGVRDMKQRHIASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVER 219

Query: 211 AAQLSVEAQVWQAKARAQEATAVSLQAQLQQAMSGGG--CDGGDGGTVGV-EGQADDAES 270
             Q+++EAQ W  +A+  E+    L+A LQQAMS         D G  G  + + DDA S
Sbjct: 220 IKQVAMEAQNWHYRAKYNESVVNVLKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAAS 279

Query: 271 AHIDPER------VVISGPSCKACRKRLASMLILPCRHLCLCTDCDQVAQTCPLCHSKRS 327
           ++IDP         +     CK C  +  S+LI+PCRHL LC +CD   + CP+C S +S
Sbjct: 280 SYIDPNNNNNNNMGIHQRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKS 339

The following BLAST results are available for this feature:

BLAST of Chy4G067600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDD1	2.0e-18	28.49	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
Q9FHE4	5.8e-18	34.12	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
F4IDI6	7.6e-18	32.43	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
O81851	1.2e-15	33.33	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q6UWE0	1.3e-04	34.48	E3 ubiquitin-protein ligase LRSAM1 OS=Homo sapiens OX=9606 GN=LRSAM1 PE=1 SV=1	[more]

BLAST of Chy4G067600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LEI4	2.6e-170	98.17	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G911260 P...	[more]
A0A1S3CRF0	5.4e-168	96.95	BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC10350349...	[more]
A0A6J1IE25	5.8e-146	85.37	BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1EXW3	2.9e-145	86.36	BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita moschata ...	[more]
A0A6J1E527	5.1e-142	86.79	BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Chy4G067600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148373.2	9.44e-218	98.17	BOI-related E3 ubiquitin-protein ligase 1 [Cucumis sativus] >KGN60440.1 hypothet...	[more]
XP_008465916.1	1.05e-214	96.95	PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo]	[more]
XP_038888350.1	3.72e-192	90.75	BOI-related E3 ubiquitin-protein ligase 1-like [Benincasa hispida]	[more]
KAG6571752.1	7.02e-186	86.45	putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrospe...	[more]
XP_022973264.1	1.11e-185	85.37	BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima]	[more]

BLAST of Chy4G067600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32740.1	5.2e-62	48.24	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G47050.1	5.0e-57	46.57	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G17680.1	4.0e-54	42.99	SBP (S-ribonuclease binding protein) family protein	[more]
AT4G17680.2	1.4e-43	44.66	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G10650.1	9.2e-27	34.27	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 180..221
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 278..316 e-value: 8.3E-8 score: 32.0
None	No IPR available	PANTHER	PTHR42647:SF30	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1-LIKE	coord: 1..325
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 258..326 e-value: 4.7E-11 score: 44.0
IPR017066	E3 ubiquitin-protein ligase BOI-like	PIRSF	PIRSF036836	SBP1_RNase_bind	coord: 1..326 e-value: 3.6E-92 score: 307.6
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..325
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 279..314 score: 9.818985

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G067600.1	Chy4G067600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity