Chy4G067330 (gene) Cucumber (hystrix) v1

Overview
NameChy4G067330
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionlow-temperature-induced 65 kDa protein
LocationchrH04: 172143 .. 174340 (-)
RNA-Seq ExpressionChy4G067330
SyntenyChy4G067330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTCACAAACCCCCCCTCATCACCATCACCATCACCACCATCCCCTTGGACTTCACCAGAGTATGACTTTCTGTGTCTCCTAACTTTTTCTTTTTTATTTTAAATACTAAGTTCATCATTTTATTTTGATTTTTGTAATGTCTTACCTTTAAATTCTTTTATGTCAAATTACATATTTGATCCATTTAAATTTGTATTTAGTTTAAATATTATTCAATAATGCTTTATAATTAATTAAGTTTTTTTTTTATAGAGATTAAATTTTAATTATAAATTTACACTAATATCTAAACCTATACAAATTATATAATTAAATTGTAGGGTGAAGTTTACTTTTCTTTAGGAATTGTGAAAGTTAACACTACTCCTCGATCAATATGGGGCAAACTTTCCGAGTAGGTTCCACACATATCAATTACGAATGTTTATATTTCATTAGATTAATAAAGAAAATTATATACAAATTTAAAAGATTTAGTATTGGTAGGTCATGTGGAGGGAAAAGAAGAACTACAAGAAGGGGATCAGGAGCATCATGAGAAGAAGTCAGTGTTGAAGAAAGTGAAGGCGAAAGCGAAGAAGATTAAAGACACTATTACGAAGCACGGCCATGGCCATGATCATCATGACCAAGAAGACGACAGTGATGACGAGGAGGATGAAGTTATAGAAGATCCTGAAATCCAGGGTGCACCAATGAGGAGTGCCGTGGCTGGGAAAGGGCAGCATCAAGATGTCGGAATTGGAACTGGAACGACGAGAATGATGCACCATGAGTCGCCTCCAGCTCGAAGGGAAATCACAAGTCGTCCCTCTGCTGTTGACACTGGCTTTACTTCCATCCATAATCCCACAGCAAATTATAAAGTATACGATTCCAATGTTGCTCCGAACACCACCATGTCTCTATCTCCATGGAAATTGGAGGAGGATCCTCACGCGCCTAAGGACCCTCATGCTCCTCATATCTCCGAGGTCAAAGTTCACGATCCCTCTAACAGAGGTAATTAGATACATACAAAGATTTTTTCGTCATCATATTCTATTTTAGAGCTCAATTCGAAGTTCATGGTACGTGTTTTATTTTGTTTAGAGCTTTGAATCTCATTCCATGATATCCAACTTCCTCGCTTTTTAATTGGGTACACATTTTATTATGCTTATCAAAATAAGTTGGCTTGCAAATTTTGTCCTATTTTTAATTTCCACTGAACTTACTTCTACCGAGTCTTTTACGTCTTCAAAGCACACTATAGAGTAAAGAGTCAACCAATCGAAGTTAGTAAAAAAAATAATCTAAAACTCAGCACTATAAGGTGATTTTTTGTTTTTGTTTTTGGGTTTTTTTTTTATCTCATGAACATGTCTGCAACGTACAGGGAGCGAAGAGGCAGCAGGGAGATCTCGAGTCTTCGATTCATTTGCAAGAATGAAAGTTAACGACAAAGAACCGAACCGAACCGGTTCACCACGTGGATTGGTCCAAGAGCAAGGAGGAGAAGACCAGACCAACTACGCCCAAAAAATCTCAGCCGTGGGTTCAGCAGTGGCAGCGAAGGATTTTGTGGCATCGAAGCTCGGGTATGGAGAAACAACAGAGGAAACCACCACCAAGAAGTCGTCTTCGTCTTCACCCTTAGAGTACGGGAAAAAAATAGCATTAACAGTGACAGAGAAGTTAAAGCCAGGGGAAGAAGACAGAGCATTGTCAGAAGTAATTTCAGAGGCTTGGACTAGAAGGAAGGATGAGGTAGTTAAAGTTGGAGAAAGTGCTTTTGGACGGCCACCCTCTAAAGGGGAGGTGACGGAGTCGGAGGAGCTGACACGGCGGCTTGGGAAAGAGGATAAGGAAGCGACAGAGAAATCCTCTGTTGCGAGTGCTGCGGCAGCGACAGGGAGGAGTGTGGTGGGTATGGTGAAAGATACGGTGGGGTCGTGGCTGGGAAATGCTGGAGAGCAATCGGCGCCATCGCAACAATCACTTGGTACTTCACAAGGTAACTTAACTAGTGTCTAGTTAAGGGTTTTGTGATTGTTCGCTTTTTTGTTTGGTTTTATCTGTTAATTTTATATTTTTGTGGGAATAATTTAAAAAGGTGTCGAGGGTTTTGTGGATTCTTCTTCATCACGGCGGCAGGCGGAGCATGGTGGTAAGGGAACGGAGGTGCGGATACTTCAGGGTTCGGCGAATTAA

mRNA sequence

ATGGACTCACAAACCCCCCCTCATCACCATCACCATCACCACCATCCCCTTGGACTTCACCAGAGTCATGTGGAGGGAAAAGAAGAACTACAAGAAGGGGATCAGGAGCATCATGAGAAGAAGTCAGTGTTGAAGAAAGTGAAGGCGAAAGCGAAGAAGATTAAAGACACTATTACGAAGCACGGCCATGGCCATGATCATCATGACCAAGAAGACGACAGTGATGACGAGGAGGATGAAGTTATAGAAGATCCTGAAATCCAGGGTGCACCAATGAGGAGTGCCGTGGCTGGGAAAGGGCAGCATCAAGATGTCGGAATTGGAACTGGAACGACGAGAATGATGCACCATGAGTCGCCTCCAGCTCGAAGGGAAATCACAAGTCGTCCCTCTGCTGTTGACACTGGCTTTACTTCCATCCATAATCCCACAGCAAATTATAAAGTATACGATTCCAATGTTGCTCCGAACACCACCATGTCTCTATCTCCATGGAAATTGGAGGAGGATCCTCACGCGCCTAAGGACCCTCATGCTCCTCATATCTCCGAGGTCAAAGTTCACGATCCCTCTAACAGAGGGAGCGAAGAGGCAGCAGGGAGATCTCGAGTCTTCGATTCATTTGCAAGAATGAAAGTTAACGACAAAGAACCGAACCGAACCGGTTCACCACGTGGATTGGTCCAAGAGCAAGGAGGAGAAGACCAGACCAACTACGCCCAAAAAATCTCAGCCGTGGGTTCAGCAGTGGCAGCGAAGGATTTTGTGGCATCGAAGCTCGGGTATGGAGAAACAACAGAGGAAACCACCACCAAGAAGTCGTCTTCGTCTTCACCCTTAGAGTACGGGAAAAAAATAGCATTAACAGTGACAGAGAAGTTAAAGCCAGGGGAAGAAGACAGAGCATTGTCAGAAGTAATTTCAGAGGCTTGGACTAGAAGGAAGGATGAGGTAGTTAAAGTTGGAGAAAGTGCTTTTGGACGGCCACCCTCTAAAGGGGAGGTGACGGAGTCGGAGGAGCTGACACGGCGGCTTGGGAAAGAGGATAAGGAAGCGACAGAGAAATCCTCTGTTGCGAGTGCTGCGGCAGCGACAGGGAGGAGTGTGGTGGGTATGGTGAAAGATACGGTGGGGTCGTGGCTGGGAAATGCTGGAGAGCAATCGGCGCCATCGCAACAATCACTTGGTACTTCACAAGGTGTCGAGGGTTTTGTGGATTCTTCTTCATCACGGCGGCAGGCGGAGCATGGTGGTAAGGGAACGGAGGTGCGGATACTTCAGGGTTCGGCGAATTAA

Coding sequence (CDS)

ATGGACTCACAAACCCCCCCTCATCACCATCACCATCACCACCATCCCCTTGGACTTCACCAGAGTCATGTGGAGGGAAAAGAAGAACTACAAGAAGGGGATCAGGAGCATCATGAGAAGAAGTCAGTGTTGAAGAAAGTGAAGGCGAAAGCGAAGAAGATTAAAGACACTATTACGAAGCACGGCCATGGCCATGATCATCATGACCAAGAAGACGACAGTGATGACGAGGAGGATGAAGTTATAGAAGATCCTGAAATCCAGGGTGCACCAATGAGGAGTGCCGTGGCTGGGAAAGGGCAGCATCAAGATGTCGGAATTGGAACTGGAACGACGAGAATGATGCACCATGAGTCGCCTCCAGCTCGAAGGGAAATCACAAGTCGTCCCTCTGCTGTTGACACTGGCTTTACTTCCATCCATAATCCCACAGCAAATTATAAAGTATACGATTCCAATGTTGCTCCGAACACCACCATGTCTCTATCTCCATGGAAATTGGAGGAGGATCCTCACGCGCCTAAGGACCCTCATGCTCCTCATATCTCCGAGGTCAAAGTTCACGATCCCTCTAACAGAGGGAGCGAAGAGGCAGCAGGGAGATCTCGAGTCTTCGATTCATTTGCAAGAATGAAAGTTAACGACAAAGAACCGAACCGAACCGGTTCACCACGTGGATTGGTCCAAGAGCAAGGAGGAGAAGACCAGACCAACTACGCCCAAAAAATCTCAGCCGTGGGTTCAGCAGTGGCAGCGAAGGATTTTGTGGCATCGAAGCTCGGGTATGGAGAAACAACAGAGGAAACCACCACCAAGAAGTCGTCTTCGTCTTCACCCTTAGAGTACGGGAAAAAAATAGCATTAACAGTGACAGAGAAGTTAAAGCCAGGGGAAGAAGACAGAGCATTGTCAGAAGTAATTTCAGAGGCTTGGACTAGAAGGAAGGATGAGGTAGTTAAAGTTGGAGAAAGTGCTTTTGGACGGCCACCCTCTAAAGGGGAGGTGACGGAGTCGGAGGAGCTGACACGGCGGCTTGGGAAAGAGGATAAGGAAGCGACAGAGAAATCCTCTGTTGCGAGTGCTGCGGCAGCGACAGGGAGGAGTGTGGTGGGTATGGTGAAAGATACGGTGGGGTCGTGGCTGGGAAATGCTGGAGAGCAATCGGCGCCATCGCAACAATCACTTGGTACTTCACAAGGTGTCGAGGGTTTTGTGGATTCTTCTTCATCACGGCGGCAGGCGGAGCATGGTGGTAAGGGAACGGAGGTGCGGATACTTCAGGGTTCGGCGAATTAA

Protein sequence

MDSQTPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITKHGHGHDHHDQEDDSDDEEDEVIEDPEIQGAPMRSAVAGKGQHQDVGIGTGTTRMMHHESPPARREITSRPSAVDTGFTSIHNPTANYKVYDSNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVAAKDFVASKLGYGETTEETTTKKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGNAGEQSAPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGTEVRILQGSAN*
Homology
BLAST of Chy4G067330 vs. ExPASy Swiss-Prot
Match: Q04980 (Low-temperature-induced 65 kDa protein OS=Arabidopsis thaliana OX=3702 GN=LTI65 PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 4.0e-14
Identity = 182/652 (27.91%), Postives = 249/652 (38.19%), Query Frame = 0

Query: 1   MDSQ-TPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDT 60
           M+SQ T P+ H     P+ +H  H E        ++EHHEK    VLKKVK KAKKIK++
Sbjct: 1   MESQLTRPYGHEQAEEPIRIH--HPE--------EEEHHEKGASKVLKKVKEKAKKIKNS 60

Query: 61  ITKHGHGHDHHDQEDDSDDEEDEVIEDPEIQGAPMRSAVAGKGQHQDVGIGTGTTRMMHH 120
           +TKHG+GHD HD EDD DDE DE  +DPE+ GAP+  + A +G     G+ TG  + + H
Sbjct: 61  LTKHGNGHD-HDVEDD-DDEYDE--QDPEVHGAPVYESSAVRG-----GV-TGKPKSLSH 120

Query: 121 ---ESPPARREIT--------------SRP----SAVDT--GFTSIHNPTANYKVYD--- 180
               + PA  EI               ++P    S  DT  G  ++ +P    +  D   
Sbjct: 121 AGETNVPASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDTSYGHEALADPVRTTETSDWEA 180

Query: 181 SNVAP------------------------NTTMSL------------------------- 240
              AP                        NT +SL                         
Sbjct: 181 KREAPTHYPLGVSEFSDRGESREAHQEPLNTPVSLLSATEDVTRTFAPGGEDDYLGGQRK 240

Query: 241 ----SPWKLEEDPHAPKDPHAPHISEV-----KVHDPSNRGSEEAAGRSRVFDSFARMKV 300
               +P +LEEDP AP    + ++S V     KV DP+++G E  AG   + +S  RMKV
Sbjct: 241 VNVETPKRLEEDPAAP-GGGSDYLSGVSNYQSKVTDPTHKGGE--AGVPEIAESLGRMKV 300

Query: 301 NDKEPNR--------------------------TGSP-----------------RGLVQE 360
            D+ P++                            SP                 RG V+ 
Sbjct: 301 TDESPDQKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGGESKAGMEEDFPTRGDVKV 360

Query: 361 QGG---------EDQ---------------------------TNYAQKISAVGS-----A 420
           + G          DQ                           + Y +++++  S     A
Sbjct: 361 ESGLGRDLPTGTHDQFSPELSRPKERDDSEETKDESTHETKPSTYTEQLASATSAITNKA 420

Query: 421 VAAKDFVASKLGY----GETTEETTTKKSSSSSPLEYGKKIALTV--------------- 423
           +AAK+ VASKLGY    G    E+  K  +  S   YG+K+A TV               
Sbjct: 421 IAAKNVVASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVAGTVAEKLTPVYEKVKETG 480

BLAST of Chy4G067330 vs. ExPASy TrEMBL
Match: A0A1S3CPX7 (low-temperature-induced 65 kDa protein OS=Cucumis melo OX=3656 GN=LOC103503460 PE=4 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 1.8e-195
Identity = 381/443 (86.00%), Postives = 396/443 (89.39%), Query Frame = 0

Query: 1   MDSQTPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQ PP  HHHHHHPLGLHQS  EGKEEL+EG +EHHEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIPP--HHHHHHPLGLHQS-AEGKEELEEG-EEHHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDHHDQEDDSDDEEDEVIEDPEIQGAP------MRSAVAGKGQHQDVGIGTGTTRM 120
           H H HDHHD+ED+ DDEEDEV+EDPEIQGAP      MRSA AGKGQHQDVGIG GTT M
Sbjct: 61  HAHAHDHHDEEDEEDDEEDEVVEDPEIQGAPLYEGAAMRSATAGKGQHQDVGIGIGTTTM 120

Query: 121 MH-HESPPARREITSRPSAVDTGFTSIHNPTANYKVYDSNVAPNTTMSLSPWKLEEDPHA 180
           MH +  PPARREI SRPSAVDTGFTS+ NPTAN+KV DSNVAPNTTMSLSPWKLEEDPH 
Sbjct: 121 MHNYAPPPARREIRSRPSAVDTGFTSVENPTANHKVDDSNVAPNTTMSLSPWKLEEDPHG 180

Query: 181 PKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDKEPNRTGSPRGLVQEQGG 240
           PKDP APHISEVKVHDPSNRGSEEAAG+S+VFDSFA+MKV+DK+PNRTGSP GL QE GG
Sbjct: 181 PKDPRAPHISEVKVHDPSNRGSEEAAGKSQVFDSFAKMKVDDKKPNRTGSPHGLDQEHGG 240

Query: 241 EDQTNYAQKISAVGS-----AVAAKDFVASKLGYGETTEETTTKKSSSSSPLEYGKKIAL 300
           ED  NYAQKISAVGS     AVAAKDFVASKLGYGET E TT  KSSSSSPLEYGKKIAL
Sbjct: 241 EDHINYAQKISAVGSAVSGKAVAAKDFVASKLGYGETKEGTTADKSSSSSPLEYGKKIAL 300

Query: 301 TVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKE 360
           TVTEKLKPGEEDRALSEVISEA  RRKDEVVKVGESAFGRPPSKG VTESEELTRRLG+E
Sbjct: 301 TVTEKLKPGEEDRALSEVISEALNRRKDEVVKVGESAFGRPPSKGVVTESEELTRRLGEE 360

Query: 361 DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGNAGEQSAPSQQSLGTSQGVEGFVDSS 420
           DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLG AGEQSAPSQQSLGTSQGVEGFVD S
Sbjct: 361 DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGTSQGVEGFVDDS 420

Query: 421 SSRRQAEHGGKGTEVRILQGSAN 432
           SSRRQAEHGG G EVR+LQGSAN
Sbjct: 421 SSRRQAEHGGTGAEVRVLQGSAN 439

BLAST of Chy4G067330 vs. ExPASy TrEMBL
Match: A0A0A0LIE7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914030 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 9.7e-173
Identity = 328/345 (95.07%), Postives = 332/345 (96.23%), Query Frame = 0

Query: 92  MRSAVAGKGQHQDVGIGTGTTRMMHHESPPARREITSRPSAVDTGFTSIHNPTANYKVYD 151
           MRSAVAGKGQHQDVGIGTGTTRMMH+E PPARREITSRPSAVDTGFTSIHN TANYKV D
Sbjct: 1   MRSAVAGKGQHQDVGIGTGTTRMMHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDD 60

Query: 152 SNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARM 211
           SNVAPNTTMSLSPWKLE+DPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRS+VFDSFARM
Sbjct: 61  SNVAPNTTMSLSPWKLEDDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARM 120

Query: 212 KVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGS-----AVAAKDFVASKLGYGETT 271
           KV+DKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGS     AVAAKDFVASKLGYGETT
Sbjct: 121 KVDDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETT 180

Query: 272 EETTTKKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAF 331
           EETTT KSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAF
Sbjct: 181 EETTTNKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAF 240

Query: 332 GRPPSKGEVTESEELTRRLGKEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGNAGE 391
           GRPPSKGEVTESEELTRRLGKEDKEATEKSSVA AAAATGRSVVGMVKDTVGSWLGNAGE
Sbjct: 241 GRPPSKGEVTESEELTRRLGKEDKEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGE 300

Query: 392 QSAPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGTEVRILQGSAN 432
           QS PSQQSLGTSQGVEGFVDSSSSRRQAEHGGKG EVR LQGSAN
Sbjct: 301 QSVPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGAEVRRLQGSAN 345

BLAST of Chy4G067330 vs. ExPASy TrEMBL
Match: A0A5D3E5L4 (Low-temperature-induced 65 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003650 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.5e-157
Identity = 306/350 (87.43%), Postives = 317/350 (90.57%), Query Frame = 0

Query: 88  QGAPMRSAVAGKGQHQDVGIGTGTTRMMH-HESPPARREITSRPSAVDTGFTSIHNPTAN 147
           +GA MRSA AGKGQHQDVGIG GTT MMH +  PPARREI SRPSAVDTGFTS+ NPTAN
Sbjct: 10  EGAAMRSATAGKGQHQDVGIGIGTTTMMHNYAPPPARREIRSRPSAVDTGFTSVENPTAN 69

Query: 148 YKVYDSNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFD 207
           +KV DSNVAPNTTMSLSPWKLEEDPH PKDP APHISEVKVHDPSNRGSEEAAG+S+VFD
Sbjct: 70  HKVDDSNVAPNTTMSLSPWKLEEDPHGPKDPRAPHISEVKVHDPSNRGSEEAAGKSQVFD 129

Query: 208 SFARMKVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGS-----AVAAKDFVASKLG 267
           SFA+MKV+DK+PNRTGSP GL QE GGED  NYAQKISAVGS     AVAAKDFVASKLG
Sbjct: 130 SFAKMKVDDKKPNRTGSPHGLDQEHGGEDHINYAQKISAVGSAVSGKAVAAKDFVASKLG 189

Query: 268 YGETTEETTTKKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKV 327
           YGET E TT  KSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEA  RRKDEVVKV
Sbjct: 190 YGETKEGTTADKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEALNRRKDEVVKV 249

Query: 328 GESAFGRPPSKGEVTESEELTRRLGKEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWL 387
           GESAFGRPPSKG VTESEELTRRLG+EDKEATEKSSVASAAAATGRSVVGMVKDTVGSWL
Sbjct: 250 GESAFGRPPSKGVVTESEELTRRLGEEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWL 309

Query: 388 GNAGEQSAPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGTEVRILQGSAN 432
           G AGEQSAPSQQSLGTSQGVEGFVD SSSRRQAEHGG G EVR+LQGSAN
Sbjct: 310 GKAGEQSAPSQQSLGTSQGVEGFVDDSSSRRQAEHGGTGAEVRVLQGSAN 359

BLAST of Chy4G067330 vs. ExPASy TrEMBL
Match: A0A6J1H2H6 (LOW QUALITY PROTEIN: low-temperature-induced 65 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111459097 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.3e-79
Identity = 216/408 (52.94%), Postives = 262/408 (64.22%), Query Frame = 0

Query: 4   QTPPHHHHHHH----HPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKD--- 63
           QT PH HH HH    H + LH+S VEG+E     D +HHEKKSVLKKVKAKAKKIKD   
Sbjct: 5   QTAPHRHHAHHHHDPHNVALHRS-VEGEE-----DGQHHEKKSVLKKVKAKAKKIKDRSL 64

Query: 64  -----TITKHGHGHDHHDQEDDSDDEEDEVIEDPEIQGAP------MRSAVAGKGQHQDV 123
                TITKHGHGHDHH+ ED  D+E    + DPE+QGAP      MRSA+AGKGQ QDV
Sbjct: 65  SMDMATITKHGHGHDHHEPEDHEDEEGGGAVVDPELQGAPLYEGAVMRSAMAGKGQQQDV 124

Query: 124 GIGTGTTRMMHHESPPARREITSRPSAVDTGFTSIHNPTANYKVYDSNVAPNTTMSLSPW 183
           GIG  T   +H + PPA RE  SRPSAV+                     PNTTMSLSPW
Sbjct: 125 GIGLPTE--VHDQPPPAPRETASRPSAVE---------------------PNTTMSLSPW 184

Query: 184 KLEEDPHAPKD----PHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDKE-PNR 243
           +LEE P APKD    PH PH SEVK  DP+ RG++E  G S+ FDSFARMK+N ++   R
Sbjct: 185 RLEEGPQAPKDKAPYPHTPHNSEVKALDPAKRGNKE-TGDSQTFDSFARMKLNHEDVQTR 244

Query: 244 TGSPRGLVQEQGGEDQTNYAQKISAVGSAVAAKDFVASKLGYGETTEETTTKKSSSSSPL 303
            G   G+  +QG  +Q++  +KIS VGS +  K             EE T   ++SSSPL
Sbjct: 245 PGLASGI--DQG--NQSSSGEKISGVGSPMRGK------------AEENT--DTNSSSPL 304

Query: 304 EYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEE 363
           EYG+KIAL VTEKLKPGEED+ALSEVISEA+   K++VVK GE+       KG+VTESEE
Sbjct: 305 EYGRKIALAVTEKLKPGEEDKALSEVISEAYNTSKEKVVKAGEN-----QPKGKVTESEE 359

Query: 364 LTRRLGKED-KEATEKSSVA-SAAAATGRSVVGMVKDTVGSWLGNAGE 387
           LT+RLG ED  E T++ S A S  AAT +++  MV+D+VGSW+G  G+
Sbjct: 365 LTQRLGGEDNNEGTQRYSPATSTTAATVKNMADMVRDSVGSWMGKGGD 359

BLAST of Chy4G067330 vs. ExPASy TrEMBL
Match: A0A6J1K662 (low-temperature-induced 65 kDa protein OS=Cucurbita maxima OX=3661 GN=LOC111491512 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 4.0e-78
Identity = 208/399 (52.13%), Postives = 260/399 (65.16%), Query Frame = 0

Query: 4   QTPPHHHHHHHHP---LGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 63
           QT PH +H HHH    + L++S  EG+E     D +HHEKKSVLKKVKAKAKKIKDTITK
Sbjct: 5   QTAPHRNHAHHHDPHNVALYRS-AEGEE-----DGQHHEKKSVLKKVKAKAKKIKDTITK 64

Query: 64  HGHGHDHHDQEDDSDDEEDEVIEDPEIQGAP------MRSAVAGKGQHQDVGIGTGTTRM 123
           HGH HDHH+ ED  ++E    + DPE+QGAP      MRSAVAGKGQ QDVGIG  T   
Sbjct: 65  HGHAHDHHEPEDHEEEEGGGAVADPELQGAPLYEGAAMRSAVAGKGQQQDVGIGLPTE-- 124

Query: 124 MHHESPPARREITSRPSAVDTGFTSIHNPTANYKVYDSNVAPNTTMSLSPWKLEEDPHAP 183
           +H + PPA R   SRPSAV+                     PNTTMSLSPW+LEE P AP
Sbjct: 125 VHDQPPPAPRGTASRPSAVE---------------------PNTTMSLSPWRLEEGPQAP 184

Query: 184 KD----PHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDKE-PNRTGSPRGLVQ 243
           +D    PH PH SEVK  DP+ RG+E+  G S+ FDSFARMK+N+++   R G   G+  
Sbjct: 185 RDKAPYPHTPHNSEVKALDPAKRGNEK-TGDSQTFDSFARMKLNNEDVQTRPGLASGI-- 244

Query: 244 EQGGEDQTNYAQKISAVGSAVAAKDFVASKLGYGETTEETTTKKSSSSSPLEYGKKIALT 303
           +QG  +Q++  +KIS  GS +  K             EE T   ++SSSPLEYG+KIAL 
Sbjct: 245 DQG--NQSSSGEKISGAGSPMLGK------------AEENT--DTNSSSPLEYGRKIALA 304

Query: 304 VTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKED 363
           VTEKLKPGEED+ALSEVISEA+   K++VVK GE+       KG+VTESEELT+RLG+ED
Sbjct: 305 VTEKLKPGEEDKALSEVISEAYNSSKEKVVKAGEN-----QPKGKVTESEELTQRLGRED 350

Query: 364 -KEATEKSSVA-SAAAATGRSVVGMVKDTVGSWLGNAGE 387
             E T++ S A S  AAT +++  +V+D+VGSW+G  G+
Sbjct: 365 NNEGTQRYSPATSTTAATVKNMADLVRDSVGSWMGKGGD 350

BLAST of Chy4G067330 vs. NCBI nr
Match: XP_011652891.1 (low-temperature-induced 65 kDa protein isoform X2 [Cucumis sativus])

HSP 1 Score: 777 bits (2007), Expect = 7.04e-282
Identity = 415/442 (93.89%), Postives = 421/442 (95.25%), Query Frame = 0

Query: 1   MDSQTPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQ PPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDHHDQEDDSDDEEDEVIEDPEIQGAP------MRSAVAGKGQHQDVGIGTGTTRM 120
           HGHGHDHHD+ED SDDEEDEV+EDPEIQGAP      MRSAVAGKGQHQDVGIGTGTTRM
Sbjct: 61  HGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIGTGTTRM 120

Query: 121 MHHESPPARREITSRPSAVDTGFTSIHNPTANYKVYDSNVAPNTTMSLSPWKLEEDPHAP 180
           MH+E PPARREITSRPSAVDTGFTSIHN TANYKV DSNVAPNTTMSLSPWKLE+DPHAP
Sbjct: 121 MHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDDSNVAPNTTMSLSPWKLEDDPHAP 180

Query: 181 KDPHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDKEPNRTGSPRGLVQEQGGE 240
           KDPHAPHISEVKVHDPSNRGSEEAAGRS+VFDSFARMKV+DKEPNRTGSPRGLVQEQGGE
Sbjct: 181 KDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVDDKEPNRTGSPRGLVQEQGGE 240

Query: 241 DQTNYAQKISAVGSAV-----AAKDFVASKLGYGETTEETTTKKSSSSSPLEYGKKIALT 300
           DQTNYAQKISAVGSAV     AAKDFVASKLGYGETTEETTT KSSSSSPLEYGKKIALT
Sbjct: 241 DQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKIALT 300

Query: 301 VTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKED 360
           VTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKED
Sbjct: 301 VTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKED 360

Query: 361 KEATEKSSVASAAAATGRSVVGMVKDTVGSWLGNAGEQSAPSQQSLGTSQGVEGFVDSSS 420
           KEATEKSSVA AAAATGRSVVGMVKDTVGSWLGNAGEQS PSQQSLGTSQGVEGFVDSSS
Sbjct: 361 KEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVDSSS 420

Query: 421 SRRQAEHGGKGTEVRILQGSAN 431
           SRRQAEHGGKG EVR LQGSAN
Sbjct: 421 SRRQAEHGGKGAEVRRLQGSAN 442

BLAST of Chy4G067330 vs. NCBI nr
Match: XP_031739118.1 (low-temperature-induced 65 kDa protein isoform X1 [Cucumis sativus])

HSP 1 Score: 771 bits (1990), Expect = 3.06e-279
Identity = 414/445 (93.03%), Postives = 421/445 (94.61%), Query Frame = 0

Query: 1   MDSQTPPHHHHHHHHPLGLHQS---HVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDT 60
           MDSQ PPHHHHHHHHPLGLHQ+   HVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDT
Sbjct: 1   MDSQIPPHHHHHHHHPLGLHQTLVGHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDT 60

Query: 61  ITKHGHGHDHHDQEDDSDDEEDEVIEDPEIQGAP------MRSAVAGKGQHQDVGIGTGT 120
           ITKHGHGHDHHD+ED SDDEEDEV+EDPEIQGAP      MRSAVAGKGQHQDVGIGTGT
Sbjct: 61  ITKHGHGHDHHDEEDVSDDEEDEVVEDPEIQGAPLYEGAAMRSAVAGKGQHQDVGIGTGT 120

Query: 121 TRMMHHESPPARREITSRPSAVDTGFTSIHNPTANYKVYDSNVAPNTTMSLSPWKLEEDP 180
           TRMMH+E PPARREITSRPSAVDTGFTSIHN TANYKV DSNVAPNTTMSLSPWKLE+DP
Sbjct: 121 TRMMHNEPPPARREITSRPSAVDTGFTSIHNTTANYKVDDSNVAPNTTMSLSPWKLEDDP 180

Query: 181 HAPKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDKEPNRTGSPRGLVQEQ 240
           HAPKDPHAPHISEVKVHDPSNRGSEEAAGRS+VFDSFARMKV+DKEPNRTGSPRGLVQEQ
Sbjct: 181 HAPKDPHAPHISEVKVHDPSNRGSEEAAGRSQVFDSFARMKVDDKEPNRTGSPRGLVQEQ 240

Query: 241 GGEDQTNYAQKISAVGSAV-----AAKDFVASKLGYGETTEETTTKKSSSSSPLEYGKKI 300
           GGEDQTNYAQKISAVGSAV     AAKDFVASKLGYGETTEETTT KSSSSSPLEYGKKI
Sbjct: 241 GGEDQTNYAQKISAVGSAVSGKAVAAKDFVASKLGYGETTEETTTNKSSSSSPLEYGKKI 300

Query: 301 ALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLG 360
           ALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLG
Sbjct: 301 ALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLG 360

Query: 361 KEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGNAGEQSAPSQQSLGTSQGVEGFVD 420
           KEDKEATEKSSVA AAAATGRSVVGMVKDTVGSWLGNAGEQS PSQQSLGTSQGVEGFVD
Sbjct: 361 KEDKEATEKSSVARAAAATGRSVVGMVKDTVGSWLGNAGEQSVPSQQSLGTSQGVEGFVD 420

Query: 421 SSSSRRQAEHGGKGTEVRILQGSAN 431
           SSSSRRQAEHGGKG EVR LQGSAN
Sbjct: 421 SSSSRRQAEHGGKGAEVRRLQGSAN 445

BLAST of Chy4G067330 vs. NCBI nr
Match: XP_008465872.2 (PREDICTED: low-temperature-induced 65 kDa protein [Cucumis melo])

HSP 1 Score: 693 bits (1788), Expect = 1.42e-248
Identity = 381/443 (86.00%), Postives = 397/443 (89.62%), Query Frame = 0

Query: 1   MDSQTPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQ PPHHH  HHHPLGLHQS  EGKEEL+EG+ EHHEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIPPHHH--HHHPLGLHQS-AEGKEELEEGE-EHHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDHHDQEDDSDDEEDEVIEDPEIQGAP------MRSAVAGKGQHQDVGIGTGTTRM 120
           H H HDHHD+ED+ DDEEDEV+EDPEIQGAP      MRSA AGKGQHQDVGIG GTT M
Sbjct: 61  HAHAHDHHDEEDEEDDEEDEVVEDPEIQGAPLYEGAAMRSATAGKGQHQDVGIGIGTTTM 120

Query: 121 MHHESPP-ARREITSRPSAVDTGFTSIHNPTANYKVYDSNVAPNTTMSLSPWKLEEDPHA 180
           MH+ +PP ARREI SRPSAVDTGFTS+ NPTAN+KV DSNVAPNTTMSLSPWKLEEDPH 
Sbjct: 121 MHNYAPPPARREIRSRPSAVDTGFTSVENPTANHKVDDSNVAPNTTMSLSPWKLEEDPHG 180

Query: 181 PKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDKEPNRTGSPRGLVQEQGG 240
           PKDP APHISEVKVHDPSNRGSEEAAG+S+VFDSFA+MKV+DK+PNRTGSP GL QE GG
Sbjct: 181 PKDPRAPHISEVKVHDPSNRGSEEAAGKSQVFDSFAKMKVDDKKPNRTGSPHGLDQEHGG 240

Query: 241 EDQTNYAQKISAVGSAV-----AAKDFVASKLGYGETTEETTTKKSSSSSPLEYGKKIAL 300
           ED  NYAQKISAVGSAV     AAKDFVASKLGYGET E TT  KSSSSSPLEYGKKIAL
Sbjct: 241 EDHINYAQKISAVGSAVSGKAVAAKDFVASKLGYGETKEGTTADKSSSSSPLEYGKKIAL 300

Query: 301 TVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPSKGEVTESEELTRRLGKE 360
           TVTEKLKPGEEDRALSEVISEA  RRKDEVVKVGESAFGRPPSKG VTESEELTRRLG+E
Sbjct: 301 TVTEKLKPGEEDRALSEVISEALNRRKDEVVKVGESAFGRPPSKGVVTESEELTRRLGEE 360

Query: 361 DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGNAGEQSAPSQQSLGTSQGVEGFVDSS 420
           DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLG AGEQSAPSQQSLGTSQGVEGFVD S
Sbjct: 361 DKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGTSQGVEGFVDDS 420

Query: 421 SSRRQAEHGGKGTEVRILQGSAN 431
           SSRRQAEHGG G EVR+LQGSAN
Sbjct: 421 SSRRQAEHGGTGAEVRVLQGSAN 439

BLAST of Chy4G067330 vs. NCBI nr
Match: KAA0038494.1 (low-temperature-induced 65 kDa protein [Cucumis melo var. makuwa] >TYK31088.1 low-temperature-induced 65 kDa protein [Cucumis melo var. makuwa])

HSP 1 Score: 568 bits (1464), Expect = 1.53e-200
Identity = 306/350 (87.43%), Postives = 318/350 (90.86%), Query Frame = 0

Query: 88  QGAPMRSAVAGKGQHQDVGIGTGTTRMMHHESPP-ARREITSRPSAVDTGFTSIHNPTAN 147
           +GA MRSA AGKGQHQDVGIG GTT MMH+ +PP ARREI SRPSAVDTGFTS+ NPTAN
Sbjct: 10  EGAAMRSATAGKGQHQDVGIGIGTTTMMHNYAPPPARREIRSRPSAVDTGFTSVENPTAN 69

Query: 148 YKVYDSNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFD 207
           +KV DSNVAPNTTMSLSPWKLEEDPH PKDP APHISEVKVHDPSNRGSEEAAG+S+VFD
Sbjct: 70  HKVDDSNVAPNTTMSLSPWKLEEDPHGPKDPRAPHISEVKVHDPSNRGSEEAAGKSQVFD 129

Query: 208 SFARMKVNDKEPNRTGSPRGLVQEQGGEDQTNYAQKISAVGSAV-----AAKDFVASKLG 267
           SFA+MKV+DK+PNRTGSP GL QE GGED  NYAQKISAVGSAV     AAKDFVASKLG
Sbjct: 130 SFAKMKVDDKKPNRTGSPHGLDQEHGGEDHINYAQKISAVGSAVSGKAVAAKDFVASKLG 189

Query: 268 YGETTEETTTKKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKV 327
           YGET E TT  KSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEA  RRKDEVVKV
Sbjct: 190 YGETKEGTTADKSSSSSPLEYGKKIALTVTEKLKPGEEDRALSEVISEALNRRKDEVVKV 249

Query: 328 GESAFGRPPSKGEVTESEELTRRLGKEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWL 387
           GESAFGRPPSKG VTESEELTRRLG+EDKEATEKSSVASAAAATGRSVVGMVKDTVGSWL
Sbjct: 250 GESAFGRPPSKGVVTESEELTRRLGEEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWL 309

Query: 388 GNAGEQSAPSQQSLGTSQGVEGFVDSSSSRRQAEHGGKGTEVRILQGSAN 431
           G AGEQSAPSQQSLGTSQGVEGFVD SSSRRQAEHGG G EVR+LQGSAN
Sbjct: 310 GKAGEQSAPSQQSLGTSQGVEGFVDDSSSRRQAEHGGTGAEVRVLQGSAN 359

BLAST of Chy4G067330 vs. NCBI nr
Match: XP_038888502.1 (low-temperature-induced 65 kDa protein isoform X2 [Benincasa hispida])

HSP 1 Score: 550 bits (1416), Expect = 4.90e-192
Identity = 341/453 (75.28%), Postives = 363/453 (80.13%), Query Frame = 0

Query: 1   MDSQTPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEKKSVLKKVKAKAKKIKDTITK 60
           MDSQ  PHHH   HHPL LHQS VEGKEE    D + HEKKSVLKKVKAKAKKIKDTITK
Sbjct: 1   MDSQIAPHHH--LHHPLALHQS-VEGKEEE---DGQLHEKKSVLKKVKAKAKKIKDTITK 60

Query: 61  HGHGHDH--HDQEDDSD-DEEDEVIEDPEIQGAP---MRSAVAGKGQHQDVGIGTGTTRM 120
           HGHGHDH  HD ED+ D DE+DEVIEDPEIQGAP   MRSAVAG+GQHQDVGI  G T  
Sbjct: 61  HGHGHDHDHHDYEDEDDEDEDDEVIEDPEIQGAPCAAMRSAVAGQGQHQDVGI--GMTTG 120

Query: 121 MHHESPPARREITSRPSAVDTGFTSIHNPTANY---KVYDSNVAPNTTMSLSPWKLEEDP 180
           MH+E     RE TSRPSA DTGFTS+ NPT N    KV DS VAPNTTMSLSPWKLEEDP
Sbjct: 121 MHNEPSRGPRETTSRPSAFDTGFTSVDNPTTNKGADKVEDSAVAPNTTMSLSPWKLEEDP 180

Query: 181 HAPKDPHAPHISEVKVHDPSNRGSEEAAGRSRVFDSFARMKVNDK-EPNRTGSPRGLVQE 240
           H P   H PH S+VKVHDP+NRGSEEA G+S+VFDSFA+MKVND+ EPNR      +  +
Sbjct: 181 HGP---HTPHNSQVKVHDPANRGSEEA-GKSQVFDSFAKMKVNDEYEPNRPSLASRI--D 240

Query: 241 QGGEDQTNYAQKISAVGSAV-----AAKDFVASKLGYGETTEETTTKKSSSSSPLEYGKK 300
           +GGEDQTNY QK+SAVGSAV     AAKDFVASKLGY ETTEET    ++SSSPLEYGKK
Sbjct: 241 RGGEDQTNYGQKVSAVGSAVSEKAVAAKDFVASKLGYVETTEETI---NNSSSPLEYGKK 300

Query: 301 IALTVTEKLKPGEEDRALSEVISEAWTRRKDEVVKVGESAFGRPPS---KGEVTESEELT 360
           IALTVTEKLKPGEED+ALSEVISEA +RRK E+VKVGESAFGR  S   KGEVTESEELT
Sbjct: 301 IALTVTEKLKPGEEDKALSEVISEALSRRKYELVKVGESAFGRHQSLLSKGEVTESEELT 360

Query: 361 RRLGKEDKEATEKSSVASAAAATGRSVVGMVKDTVGSWLGNAGEQSAPSQQSLGTSQGVE 420
           RRLGKEDKEATEKSSVA A AATGRSVVGMVKDTVGSWLG AGEQSAPSQQSLG SQGVE
Sbjct: 361 RRLGKEDKEATEKSSVAGATAATGRSVVGMVKDTVGSWLGKAGEQSAPSQQSLGISQGVE 420

Query: 421 GFVDSSS----SRRQAEHGGKGTEVRILQGSAN 431
           GFVDSSS     RRQ EH G   +VRILQ SAN
Sbjct: 421 GFVDSSSVGEGRRRQQEHAGTEPDVRILQDSAN 436

BLAST of Chy4G067330 vs. TAIR 10
Match: AT5G52300.1 (CAP160 protein )

HSP 1 Score: 80.9 bits (198), Expect = 2.8e-15
Identity = 182/652 (27.91%), Postives = 249/652 (38.19%), Query Frame = 0

Query: 1   MDSQ-TPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDT 60
           M+SQ T P+ H     P+ +H  H E        ++EHHEK    VLKKVK KAKKIK++
Sbjct: 1   MESQLTRPYGHEQAEEPIRIH--HPE--------EEEHHEKGASKVLKKVKEKAKKIKNS 60

Query: 61  ITKHGHGHDHHDQEDDSDDEEDEVIEDPEIQGAPMRSAVAGKGQHQDVGIGTGTTRMMHH 120
           +TKHG+GHD HD EDD DDE DE  +DPE+ GAP+  + A +G     G+ TG  + + H
Sbjct: 61  LTKHGNGHD-HDVEDD-DDEYDE--QDPEVHGAPVYESSAVRG-----GV-TGKPKSLSH 120

Query: 121 ---ESPPARREIT--------------SRP----SAVDT--GFTSIHNPTANYKVYD--- 180
               + PA  EI               ++P    S  DT  G  ++ +P    +  D   
Sbjct: 121 AGETNVPASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDTSYGHEALADPVRTTETSDWEA 180

Query: 181 SNVAP------------------------NTTMSL------------------------- 240
              AP                        NT +SL                         
Sbjct: 181 KREAPTHYPLGVSEFSDRGESREAHQEPLNTPVSLLSATEDVTRTFAPGGEDDYLGGQRK 240

Query: 241 ----SPWKLEEDPHAPKDPHAPHISEV-----KVHDPSNRGSEEAAGRSRVFDSFARMKV 300
               +P +LEEDP AP    + ++S V     KV DP+++G E  AG   + +S  RMKV
Sbjct: 241 VNVETPKRLEEDPAAP-GGGSDYLSGVSNYQSKVTDPTHKGGE--AGVPEIAESLGRMKV 300

Query: 301 NDKEPNR--------------------------TGSP-----------------RGLVQE 360
            D+ P++                            SP                 RG V+ 
Sbjct: 301 TDESPDQKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGGESKAGMEEDFPTRGDVKV 360

Query: 361 QGG---------EDQ---------------------------TNYAQKISAVGS-----A 420
           + G          DQ                           + Y +++++  S     A
Sbjct: 361 ESGLGRDLPTGTHDQFSPELSRPKERDDSEETKDESTHETKPSTYTEQLASATSAITNKA 420

Query: 421 VAAKDFVASKLGY----GETTEETTTKKSSSSSPLEYGKKIALTV--------------- 423
           +AAK+ VASKLGY    G    E+  K  +  S   YG+K+A TV               
Sbjct: 421 IAAKNVVASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVAGTVAEKLTPVYEKVKETG 480

BLAST of Chy4G067330 vs. TAIR 10
Match: AT5G52300.2 (CAP160 protein )

HSP 1 Score: 80.9 bits (198), Expect = 2.8e-15
Identity = 180/652 (27.61%), Postives = 247/652 (37.88%), Query Frame = 0

Query: 1   MDSQ-TPPHHHHHHHHPLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDT 60
           M+SQ T P+ H     P+ +H             ++EHHEK    VLKKVK KAKKIK++
Sbjct: 1   MESQLTRPYGHEQAEEPIRIHHP-----------EEEHHEKGASKVLKKVKEKAKKIKNS 60

Query: 61  ITKHGHGHDHHDQEDDSDDEEDEVIEDPEIQGAPMRSAVAGKGQHQDVGIGTGTTRMMHH 120
           +TKHG+GHD HD EDD DDE DE  +DPE+ GAP+  + A +G     G+ TG  + + H
Sbjct: 61  LTKHGNGHD-HDVEDD-DDEYDE--QDPEVHGAPVYESSAVRG-----GV-TGKPKSLSH 120

Query: 121 ---ESPPARREIT--------------SRP----SAVDT--GFTSIHNPTANYKVYD--- 180
               + PA  EI               ++P    S  DT  G  ++ +P    +  D   
Sbjct: 121 AGETNVPASEEIVPPGTKVFPVVSSDHTKPIEPVSLQDTSYGHEALADPVRTTETSDWEA 180

Query: 181 SNVAP------------------------NTTMSL------------------------- 240
              AP                        NT +SL                         
Sbjct: 181 KREAPTHYPLGVSEFSDRGESREAHQEPLNTPVSLLSATEDVTRTFAPGGEDDYLGGQRK 240

Query: 241 ----SPWKLEEDPHAPKDPHAPHISEV-----KVHDPSNRGSEEAAGRSRVFDSFARMKV 300
               +P +LEEDP AP    + ++S V     KV DP+++G E  AG   + +S  RMKV
Sbjct: 241 VNVETPKRLEEDPAAP-GGGSDYLSGVSNYQSKVTDPTHKGGE--AGVPEIAESLGRMKV 300

Query: 301 NDKEPNR--------------------------TGSP-----------------RGLVQE 360
            D+ P++                            SP                 RG V+ 
Sbjct: 301 TDESPDQKSRQGREEDFPTRSHEFDLKKESDINKNSPARFGGESKAGMEEDFPTRGDVKV 360

Query: 361 QGG---------EDQ---------------------------TNYAQKISAVGS-----A 420
           + G          DQ                           + Y +++++  S     A
Sbjct: 361 ESGLGRDLPTGTHDQFSPELSRPKERDDSEETKDESTHETKPSTYTEQLASATSAITNKA 420

Query: 421 VAAKDFVASKLGY----GETTEETTTKKSSSSSPLEYGKKIALTV--------------- 423
           +AAK+ VASKLGY    G    E+  K  +  S   YG+K+A TV               
Sbjct: 421 IAAKNVVASKLGYTGENGGGQSESPVKDETPRSVTAYGQKVAGTVAEKLTPVYEKVKETG 480

BLAST of Chy4G067330 vs. TAIR 10
Match: AT5G52310.1 (low-temperature-responsive protein 78 (LTI78) / desiccation-responsive protein 29A (RD29A) )

HSP 1 Score: 45.1 bits (105), Expect = 1.7e-04
Identity = 70/266 (26.32%), Postives = 105/266 (39.47%), Query Frame = 0

Query: 16  PLGLHQSHVEGKEELQEGDQEHHEK--KSVLKKVKAKAKKIKDTITKHGHGHDH---HD- 75
           PL  HQ H E        + EHHE     + +KVKA+AKK K+++TKHG  ++H   HD 
Sbjct: 8   PLNTHQQHPE--------EVEHHENGATKMFRKVKARAKKFKNSLTKHGQSNEHEQDHDL 67

Query: 76  -QEDDSDDE-EDEVIEDPEIQGAPMRSAV------------------------------- 135
            +EDD DDE E EVI+ P + G P  + V                               
Sbjct: 68  VEEDDDDDELEPEVIDAPGVTGKPRETNVPASEEIIPPGTKVFPVVSSDYTKPTESVPVQ 127

Query: 136 ------------------AGKGQHQDVGIGTGTTRMM-HHESPPARREITSRPSAVDTG- 195
                             + K + +DV I    + +    ES     E  + P ++ +G 
Sbjct: 128 EASYGHDAPAHSVRTTFTSDKEEKRDVPIHHPLSELSDREESRETHHESLNTPVSLLSGT 187

Query: 196 --FTSIHNPTANYKVYDSNVAPNTTMSLSPWKLEEDPHAPKDPHAPHISEVKVHDPSNRG 221
              TS   P+ + +  D     N     +P  LEE+             + KV DP+   
Sbjct: 188 EDVTSTFAPSGDDEYLDGQRKVNVE---TPITLEEESAVSDYLSGVSNYQSKVTDPT--- 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q049804.0e-1427.91Low-temperature-induced 65 kDa protein OS=Arabidopsis thaliana OX=3702 GN=LTI65 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CPX71.8e-19586.00low-temperature-induced 65 kDa protein OS=Cucumis melo OX=3656 GN=LOC103503460 P... [more]
A0A0A0LIE79.7e-17395.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914030 PE=4 SV=1[more]
A0A5D3E5L41.5e-15787.43Low-temperature-induced 65 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1H2H61.3e-7952.94LOW QUALITY PROTEIN: low-temperature-induced 65 kDa protein-like OS=Cucurbita mo... [more]
A0A6J1K6624.0e-7852.13low-temperature-induced 65 kDa protein OS=Cucurbita maxima OX=3661 GN=LOC1114915... [more]
Match NameE-valueIdentityDescription
XP_011652891.17.04e-28293.89low-temperature-induced 65 kDa protein isoform X2 [Cucumis sativus][more]
XP_031739118.13.06e-27993.03low-temperature-induced 65 kDa protein isoform X1 [Cucumis sativus][more]
XP_008465872.21.42e-24886.00PREDICTED: low-temperature-induced 65 kDa protein [Cucumis melo][more]
KAA0038494.11.53e-20087.43low-temperature-induced 65 kDa protein [Cucumis melo var. makuwa] >TYK31088.1 lo... [more]
XP_038888502.14.90e-19275.28low-temperature-induced 65 kDa protein isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G52300.12.8e-1527.91CAP160 protein [more]
AT5G52300.22.8e-1527.61CAP160 protein [more]
AT5G52310.11.7e-0426.32low-temperature-responsive protein 78 (LTI78) / desiccation-responsive protein 2... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..355
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 380..431
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 263..282
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 161..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..200
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 19..42
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 259..282
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..367
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 381..411
NoneNo IPR availablePANTHERPTHR33836:SF15SUBFAMILY NOT NAMEDcoord: 101..287
coord: 285..402
IPR037491Low-temperature-induced 78kDa/65kDaPANTHERPTHR33836LOW-TEMPERATURE-INDUCED 65 KDA PROTEIN-RELATEDcoord: 285..402
IPR037491Low-temperature-induced 78kDa/65kDaPANTHERPTHR33836LOW-TEMPERATURE-INDUCED 65 KDA PROTEIN-RELATEDcoord: 101..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G067330.1Chy4G067330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009737 response to abscisic acid