Homology
BLAST of Chy3G065550 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 480.3 bits (1235), Expect = 2.7e-134
Identity = 250/507 (49.31%), Postives = 347/507 (68.44%), Query Frame = 0
Query: 15 LLPVFPPIIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSA 74
LL F ++++ L F S +R LPPS PG + P++GHL+L+ LHR+L LS
Sbjct: 6 LLYTFLAVVLLSISLKLFPVSRR--RRNLPPS-PGLALPVIGHLHLIGKLLHRSLYDLSK 65
Query: 75 KHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYG 134
K+G + L G+R VL++SSP+AA+EC TKNDI FA+RP +GK++G NYT ++ +PYG
Sbjct: 66 KYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYG 125
Query: 135 PHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETV 194
HWRN RR+A ++I S ++ + S+R +EVK L+ L +G++ F VEMK E
Sbjct: 126 EHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQD-FGKVEMKSKLSELS 185
Query: 195 LNVMMRMIAGKRYCGER-ESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIE 254
NV MRM+AGKRY G+ +S+EA+ FR ++ E F+ +GA+N GDF+P FL+W+ + E
Sbjct: 186 FNVTMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVP-FLRWID-FKNYE 245
Query: 255 KRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRG 314
K++ ++ D F+Q LI E R K + M D LL LQ+++P YYTD I+G
Sbjct: 246 KKVSKISQEMDAFLQRLIHESRI--------NKNNVTMIDHLLSLQESQPEYYTDQIIKG 305
Query: 315 MMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLN 374
++ +L AGTDTS+ T+EWA+SLLLNHP +L KA+ EI+T VG NRL++E DL L +L+
Sbjct: 306 IIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLH 365
Query: 375 SVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKP 434
+++ ET R+ P AP+LVPHESS DC V G+ VP+GT+LLVN WAI D + W +P FKP
Sbjct: 366 NIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKP 425
Query: 435 ERFQDAGEVE-EGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMS 494
ER G VE E + +PFG GRR CPG GLA R+VGLTLG LIQCFEW+R+GE +DM+
Sbjct: 426 ERH---GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMA 485
Query: 495 EGGGLTMPRARPLWANYRPRPILLRLL 520
EG GLTMP+A+PL A +PR IL +++
Sbjct: 486 EGSGLTMPKAQPLEALCKPRNILHKVV 495
BLAST of Chy3G065550 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 451.1 bits (1159), Expect = 1.8e-125
Identity = 236/497 (47.48%), Postives = 322/497 (64.79%), Query Frame = 0
Query: 22 IIIIIFLLLRFLRSNNTGQ--RRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHGPI 81
II + FL++ NT + + LPP P PI+G+L+ LK PLH T LS K+G I
Sbjct: 9 IISLFFLIITLKVFFNTSRKFKNLPPGPQ--CLPIIGNLHQLKQPLHHTFHTLSQKYGQI 68
Query: 82 LLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRN 141
L FGSR V+++SS + AQEC TKNDI A+RP GK++G N T + +PYG HWRN
Sbjct: 69 FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 128
Query: 142 QRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVLNVMM 201
RRI +++ILS R++ + +R +E+ LI++L NGF VE++ +F E N +M
Sbjct: 129 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQ-KSYNGFTEVELRPMFSEMTFNTIM 188
Query: 202 RMIAGKRY----CGERESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKR 261
RM++GKRY C + EEA FR I+KE + GA N+GDFL FL+W + G+EKR
Sbjct: 189 RMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFL-GFLRWFD-FDGLEKR 248
Query: 262 MERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMM 321
++++ D F+Q LI+E R +R+ S M D LL QQ++P YYTD I+G+M
Sbjct: 249 LKKISKRTDAFLQGLIDEHRFGKRN-------SNTMIDHLLTQQQSQPEYYTDQIIKGLM 308
Query: 322 QGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSV 381
ML AGTDTSS T+EWA+S LLNHP + KA+ E+DTH+G +R + E D+S LP+L S+
Sbjct: 309 VVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSI 368
Query: 382 LKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPER 441
+ ETLR++ APLLVPH SS+D +GG+++P+ T+L+VN W I D + W DP FKPER
Sbjct: 369 VYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPER 428
Query: 442 FQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGG 501
F+ GEV + L FG GRR CPGE L+ R GLTLG LIQCFEW+R+GEE +DM E
Sbjct: 429 FEKEGEVN---KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAK 488
Query: 502 GLTMPRARPLWANYRPR 513
G+T + L A + R
Sbjct: 489 GITAGKKTSLNAMCKVR 490
BLAST of Chy3G065550 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 446.0 bits (1146), Expect = 5.7e-124
Identity = 239/498 (47.99%), Postives = 318/498 (63.86%), Query Frame = 0
Query: 23 IIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHGPILLL 82
II I +LL+ + + LPP PP + PI+G+L+ LK+PLHRT LS +G I L
Sbjct: 13 IITIKILLKI---TSRRLKNLPPGPP--TIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSL 72
Query: 83 HFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQRR 142
FGSR V+++SSPS A EC TKNDI A+RPR GK++ NYT + A YG HWRN RR
Sbjct: 73 WFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRR 132
Query: 143 IATLQILSPARIHMYGSVRTEEVKFLIRRLC-GCTGEN-GFRMVEMKKVFFETVLNVMMR 202
I T+ +LS R++ + VR +E LI++L E GF VE++ E N MMR
Sbjct: 133 ITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMR 192
Query: 203 MIAGKRYCGE----RESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRM 262
MI+GKRY G+ + EEA++FREI+ E + GA N GDFLP L+ V L +EKR
Sbjct: 193 MISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLP-LLRVVDL-DNLEKRC 252
Query: 263 ERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQ 322
+R+ + F++ LIEE R +G+ + M D LL L +++P YY+D I+G++Q
Sbjct: 253 KRIAKRSNAFLEGLIEEHR-----RGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 312
Query: 323 GMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVL 382
GML AGTDTS+ T+EW +S LLNHP L KA+EE+DT +G N+L+ E DLS LP+L +++
Sbjct: 313 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 372
Query: 383 KETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERF 442
ETLR++P APLL+PH SS+DC +G F+VP+ T++L N W I D W D FKPERF
Sbjct: 373 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF 432
Query: 443 QDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGG 502
+ EV + + FG GRR CPG LA R VG T+G LIQCFEW R EE +DM EG G
Sbjct: 433 EKEEEVN---KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKG 492
Query: 503 LTMPRARPLWANYRPRPI 515
+TMP PL A + PI
Sbjct: 493 ITMPMKIPLRAMCKALPI 495
BLAST of Chy3G065550 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 436.4 bits (1121), Expect = 4.5e-121
Identity = 235/506 (46.44%), Postives = 329/506 (65.02%), Query Frame = 0
Query: 22 IIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSA-----KH 81
I +IFL++ F + ++ LPPSPPG PI+GHL LLK P+HRTL S
Sbjct: 13 IFSLIFLIISF-KFLKPKKQNLPPSPPGW-LPIIGHLRLLKPPIHRTLRSFSETLDHNDG 72
Query: 82 GPILLLHFGSRPVLLISSPS-AAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGP 141
G ++ L GSR V ++SS AA+EC KND+ A+RP++ +GKH+G N TN++ APYG
Sbjct: 73 GGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGD 132
Query: 142 HWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVL 201
HWRN RR+ T++I S R++ + VRT+EV+ LI RL G +VE+K + +
Sbjct: 133 HWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK-TVVELKPMLMDLTF 192
Query: 202 NVMMRMIAGKRYCGER--ESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIE 261
N +MRM+ GKRY GE + EEA+R R++V + + + N D++P + L+S E
Sbjct: 193 NNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVP----ILRLFSSYE 252
Query: 262 KRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRG 321
R+++L D F+Q LI+ DK + + M D LL LQ+++ YYTD I+G
Sbjct: 253 NRVKKLGEETDKFLQGLID-------DKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKG 312
Query: 322 MMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLN 381
++ M+ AGT+TS+ T+EWALS LLNHP+ + KA++EID VG +RL++E+DLS LP+L
Sbjct: 313 IILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLK 372
Query: 382 SVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKP 441
+++ ETLR++P PLLVPH +S+DC +G + +PRGT LLVN WAI D ++W DP FKP
Sbjct: 373 NIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKP 432
Query: 442 ERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSE 501
ERF+ + EE + L FG GRR CPG GLA R+VGL LG LIQCFEW RVG VDM E
Sbjct: 433 ERFE---KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKE 492
Query: 502 GGGLTMPRARPLWANYRPRPILLRLL 520
G G T+P+A PL A + RP L +++
Sbjct: 493 GVGNTVPKAIPLKAICKARPFLHKII 501
BLAST of Chy3G065550 vs. ExPASy Swiss-Prot
Match:
Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)
HSP 1 Score: 433.3 bits (1113), Expect = 3.8e-120
Identity = 232/502 (46.22%), Postives = 319/502 (63.55%), Query Frame = 0
Query: 26 IFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKL--SAKHGPILLLH 85
IFL L+ L + LPPS P FPI+GHL+LLK PLHR L S + I L
Sbjct: 22 IFLSLKLLFGPRPRKLNLPPS-PSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLS 81
Query: 86 FGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQRRI 145
GSR V ++SS + A+EC TKND+ A+RP VGKH+G N T ++ A YG WRN RRI
Sbjct: 82 LGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLRRI 141
Query: 146 ATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGEN---GFRMVEMKKVFFETVLNVMMR 205
T++I S R++ + S+R +E IRRL C +N GF VEMK +F +N ++R
Sbjct: 142 GTIEIFSSLRLNSFVSIRQDE----IRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIR 201
Query: 206 MIAGKRYCGE--RESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMER 265
M+AGKR+ G+ EA+ R+++ E GA N D+ P L++V + EK +++
Sbjct: 202 MVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFP-ILRYV---TNYEKHVKK 261
Query: 266 LQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGM 325
L G D F+Q+L+ E+R E + M D LL LQ+T+P YYTD I+G++ M
Sbjct: 262 LAGRVDEFLQSLVNEKRV-------EKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVM 321
Query: 326 LSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLKE 385
+ AGTDTS+ T+EWA+S LLNHP L KA+ EID +G +RL++E D+ LP+L ++ E
Sbjct: 322 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 381
Query: 386 TLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQD 445
TLR+YPVAP+L+PH +S+DC+V G+ VPRGT++LVN WAI D W +P +FKPERF+
Sbjct: 382 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 441
Query: 446 AGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEG-GGL 505
GE + + +PFG GRR CPG GLA R+V L LG L+QCFEW RV E+ +DM E G
Sbjct: 442 KGEDK---KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGT 501
Query: 506 TMPRARPLWANYRPRPILLRLL 520
TM +A L A + RPI+ ++L
Sbjct: 502 TMRKATSLQAMCKARPIVHKVL 504
BLAST of Chy3G065550 vs. ExPASy TrEMBL
Match:
A0A0A0LR62 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354920 PE=3 SV=1)
HSP 1 Score: 1050.4 bits (2715), Expect = 2.4e-303
Identity = 514/522 (98.47%), Postives = 515/522 (98.66%), Query Frame = 0
Query: 1 MFQFVGLFLHFLDQLLPVFPPIIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNL 60
MFQFVGLFLHFLDQLL +FP IIIIIFLLLRFLRSNN GQRRLPPSPPGGSFPILGHLNL
Sbjct: 1 MFQFVGLFLHFLDQLLLLFPLIIIIIFLLLRFLRSNNPGQRRLPPSPPGGSFPILGHLNL 60
Query: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH
Sbjct: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
Query: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENG 180
LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCG TGENG
Sbjct: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGGTGENG 180
Query: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP
Sbjct: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
Query: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQ 300
NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERR MRRDKGDEMKESRVMADVLLDLQQ
Sbjct: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRRMRRDKGDEMKESRVMADVLLDLQQ 300
Query: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWA SLLLNHPNSLLK QEEIDTHVGPNRL
Sbjct: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWAFSLLLNHPNSLLKVQEEIDTHVGPNRL 360
Query: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN
Sbjct: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
Query: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE
Sbjct: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
Query: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF 523
WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF
Sbjct: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF 522
BLAST of Chy3G065550 vs. ExPASy TrEMBL
Match:
A0A5A7V256 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00700 PE=3 SV=1)
HSP 1 Score: 995.0 bits (2571), Expect = 1.2e-286
Identity = 490/522 (93.87%), Postives = 499/522 (95.59%), Query Frame = 0
Query: 1 MFQFVGLFLHFLDQLLPVFPPIIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNL 60
MF+FVGLFL FLDQLL VFPPIIII LLR L+S NTG+ RLPPSPPGGSFPILGHL L
Sbjct: 1 MFRFVGLFLPFLDQLLLVFPPIIII--FLLRLLQSKNTGRERLPPSPPGGSFPILGHLYL 60
Query: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
LKNPLHRTLAKLSA+HGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH
Sbjct: 61 LKNPLHRTLAKLSAQHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
Query: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENG 180
LGCNYTNILWAPYGPHWRN RRIATLQILSP R+HMYGSVR EEVKFLIRRLCG GENG
Sbjct: 121 LGCNYTNILWAPYGPHWRNLRRIATLQILSPNRVHMYGSVRREEVKFLIRRLCGGIGENG 180
Query: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP
Sbjct: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
Query: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQ 300
+FLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERR MRRD GDEMKESRVMADVLLDLQQ
Sbjct: 241 SFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRRMRRDNGDEMKESRVMADVLLDLQQ 300
Query: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
TEPHYYTDAFIR MMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL
Sbjct: 301 TEPHYYTDAFIRCMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
Query: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
LQESDLSNLPFL+SVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN
Sbjct: 361 LQESDLSNLPFLHSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
Query: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
D DSWPDPAEF+PERFQD GEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLG LIQCFE
Sbjct: 421 DGDSWPDPAEFRPERFQDVGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGSLIQCFE 480
Query: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF 523
WRRVGEEMVDMSEGGGLTMPRA+P WA RPRPILLRLLPTF
Sbjct: 481 WRRVGEEMVDMSEGGGLTMPRAKPCWAKCRPRPILLRLLPTF 520
BLAST of Chy3G065550 vs. ExPASy TrEMBL
Match:
A0A1S3BBE3 (isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103487855 PE=3 SV=1)
HSP 1 Score: 995.0 bits (2571), Expect = 1.2e-286
Identity = 490/522 (93.87%), Postives = 499/522 (95.59%), Query Frame = 0
Query: 1 MFQFVGLFLHFLDQLLPVFPPIIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNL 60
MF+FVGLFL FLDQLL VFPPIIII LLR L+S NTG+ RLPPSPPGGSFPILGHL L
Sbjct: 1 MFRFVGLFLPFLDQLLLVFPPIIII--FLLRLLQSKNTGRERLPPSPPGGSFPILGHLYL 60
Query: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
LKNPLHRTLAKLSA+HGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH
Sbjct: 61 LKNPLHRTLAKLSAQHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
Query: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENG 180
LGCNYTNILWAPYGPHWRN RRIATLQILSP R+HMYGSVR EEVKFLIRRLCG GENG
Sbjct: 121 LGCNYTNILWAPYGPHWRNLRRIATLQILSPNRVHMYGSVRREEVKFLIRRLCGGIGENG 180
Query: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP
Sbjct: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
Query: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQ 300
+FLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERR MRRD GDEMKESRVMADVLLDLQQ
Sbjct: 241 SFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRRMRRDNGDEMKESRVMADVLLDLQQ 300
Query: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
TEPHYYTDAFIR MMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL
Sbjct: 301 TEPHYYTDAFIRCMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
Query: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
LQESDLSNLPFL+SVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN
Sbjct: 361 LQESDLSNLPFLHSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
Query: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
D DSWPDPAEF+PERFQD GEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLG LIQCFE
Sbjct: 421 DGDSWPDPAEFRPERFQDVGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGSLIQCFE 480
Query: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF 523
WRRVGEEMVDMSEGGGLTMPRA+P WA RPRPILLRLLPTF
Sbjct: 481 WRRVGEEMVDMSEGGGLTMPRAKPCWAKCRPRPILLRLLPTF 520
BLAST of Chy3G065550 vs. ExPASy TrEMBL
Match:
A0A5B6WTN2 (Isoflavone 2'-hydroxylase-like OS=Gossypium australe OX=47621 GN=EPI10_006814 PE=3 SV=1)
HSP 1 Score: 614.0 bits (1582), Expect = 5.8e-172
Identity = 305/500 (61.00%), Postives = 376/500 (75.20%), Query Frame = 0
Query: 24 IIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHGPILLLH 83
++ FL+ FL R LPPS P S PI+GHL+L+K PLHRTLAKLS +HGPIL L
Sbjct: 9 VLFFLIFYFLTQRLLQNRGLPPS-PALSLPIIGHLHLIKKPLHRTLAKLSKQHGPILFLR 68
Query: 84 FGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQRRI 143
FGSRPVL++SSPSA +ECL+KND FA+RPRL GKHLG +YT ++WAPYG HWRN RR+
Sbjct: 69 FGSRPVLVVSSPSATEECLSKNDTVFANRPRLLAGKHLGYDYTTLVWAPYGDHWRNLRRV 128
Query: 144 ATLQILSPARIHMYGSVRTEEVKFLIRRLCGCT--GENGFRMVEMKKVFFETVLNVMMRM 203
A+LQ+LS R+ Y +R +EVK L+ RL T G F++VEMK +FFE LNVMMRM
Sbjct: 129 ASLQLLSSNRVQKYLGIRMDEVKSLVVRLFRSTSKGSGEFQVVEMKSMFFELTLNVMMRM 188
Query: 204 IAGKRYC--GERESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMERL 263
IAGKRYC GE E EE ++F+EIV+E F+VSGATN+ DF+P LKWVGL + IEK++E L
Sbjct: 189 IAGKRYCRDGEDELEEEKKFKEIVRESFQVSGATNIVDFVP-MLKWVGL-NKIEKKLEIL 248
Query: 264 QGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGML 323
Q RD FMQNLI+ERR + + S+ + DVLL Q+T+P YYTD IRG MQ +L
Sbjct: 249 QRKRDEFMQNLIDERRKLTSSNSCYEQNSKTIVDVLLSAQETDPEYYTDDAIRGFMQVLL 308
Query: 324 SAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLKET 383
SAGTDTS+ATMEW LSLLLN+P +L KA+EEID VG +RL+ ESD L +L++V+ ET
Sbjct: 309 SAGTDTSAATMEWGLSLLLNNPKTLEKAREEIDMEVGQSRLIHESDYERLAYLHAVISET 368
Query: 384 LRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQDA 443
R+ P PLLVPHESS++C+VGGF +PRGTMLLVN WA+ ND W +P EFKP+RF
Sbjct: 369 FRMCPPGPLLVPHESSEECMVGGFSIPRGTMLLVNIWALHNDPVLWENPTEFKPDRFLGP 428
Query: 444 GEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGLTM 503
G V+ G +LPFG GRRGCPGE LAM ++ LT+GCLIQCFEW R+GEEMVDMSEG GL M
Sbjct: 429 GLVKNGFTFLPFGTGRRGCPGESLAMGLIPLTVGCLIQCFEWERMGEEMVDMSEGNGLNM 488
Query: 504 PRARPLWANYRPRPILLRLL 520
P+A+PL A YRPRP ++ LL
Sbjct: 489 PKAQPLVARYRPRPAMMNLL 505
BLAST of Chy3G065550 vs. ExPASy TrEMBL
Match:
V4T5X6 (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10000918mg PE=3 SV=1)
HSP 1 Score: 613.2 bits (1580), Expect = 9.9e-172
Identity = 313/495 (63.23%), Postives = 378/495 (76.36%), Query Frame = 0
Query: 27 FLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHGPILLLHFGS 86
FL FL+ N R LPPS PG S PI+GHL L K PLHRTLAKLS ++GPIL FGS
Sbjct: 17 FLTKYFLQVN----RNLPPS-PGVSLPIIGHLYLFKKPLHRTLAKLSNQYGPILFTRFGS 76
Query: 87 RPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQRRIATL 146
RPV+L+SSPSAA+EC TKNDI FA+RPRL GKHLG NYT ++WAPYG HWRN RRIA+L
Sbjct: 77 RPVVLVSSPSAAEECFTKNDIIFANRPRLLAGKHLGYNYTTLVWAPYGDHWRNLRRIASL 136
Query: 147 QILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVLNVMMRMIAGKR 206
++LS R+ M+ +R +EVK L +RL + +R+ +MK +FFE LNV+MRMIAGKR
Sbjct: 137 ELLSSNRLQMFHGIRVDEVKLLSQRLFRGSKSGEYRIFDMKSMFFELTLNVVMRMIAGKR 196
Query: 207 YCGER--ESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMERLQGLRD 266
Y GE SE+A RF+ IV E F++SGATN+ D LP F+KWVGL S EKR+ L G RD
Sbjct: 197 YYGENVSGSEDARRFKAIVTETFQLSGATNVVDLLP-FVKWVGL-SKTEKRLMILHGKRD 256
Query: 267 GFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGMLSAGTD 326
FMQ+LIEERR +R D E + SR M DVLL LQ+TEP YYTD IRGMMQ +L AG+D
Sbjct: 257 KFMQDLIEERRRLRCDSASEQR-SRTMIDVLLSLQETEPEYYTDHIIRGMMQVLLGAGSD 316
Query: 327 TSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLKETLRIYP 386
TS+ATMEWALS LLN+P L++AQ EIDT+VG +RL++ESDL+ LP+L+ V+KETLR+YP
Sbjct: 317 TSAATMEWALSSLLNNPEILVQAQSEIDTNVGQSRLIEESDLTALPYLHGVIKETLRMYP 376
Query: 387 VAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQDAGEVEE 446
APLLVPHESS++C +GGF VPRGTMLLVN+WAIQND W +P++FKPERF++ +
Sbjct: 377 AAPLLVPHESSEECTIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFREMAGKSD 436
Query: 447 GLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGLTMPRARP 506
LPFG GRR CPGEGLAMR+VGL LG LIQCFEW R G E+VDMSEG GLTMPRA+P
Sbjct: 437 KYMLLPFGTGRRVCPGEGLAMRLVGLALGSLIQCFEWVRTGGELVDMSEGTGLTMPRAQP 496
Query: 507 LWANYRPRPILLRLL 520
L A RPRP ++ LL
Sbjct: 497 LLAMCRPRPAMVTLL 503
BLAST of Chy3G065550 vs. NCBI nr
Match:
XP_004143022.1 (cytochrome P450 81Q32 [Cucumis sativus] >KGN62466.1 hypothetical protein Csa_018787 [Cucumis sativus])
HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 514/522 (98.47%), Postives = 515/522 (98.66%), Query Frame = 0
Query: 1 MFQFVGLFLHFLDQLLPVFPPIIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNL 60
MFQFVGLFLHFLDQLL +FP IIIIIFLLLRFLRSNN GQRRLPPSPPGGSFPILGHLNL
Sbjct: 1 MFQFVGLFLHFLDQLLLLFPLIIIIIFLLLRFLRSNNPGQRRLPPSPPGGSFPILGHLNL 60
Query: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH
Sbjct: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
Query: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENG 180
LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCG TGENG
Sbjct: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGGTGENG 180
Query: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP
Sbjct: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
Query: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQ 300
NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERR MRRDKGDEMKESRVMADVLLDLQQ
Sbjct: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRRMRRDKGDEMKESRVMADVLLDLQQ 300
Query: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWA SLLLNHPNSLLK QEEIDTHVGPNRL
Sbjct: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWAFSLLLNHPNSLLKVQEEIDTHVGPNRL 360
Query: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN
Sbjct: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
Query: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE
Sbjct: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
Query: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF 522
WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF
Sbjct: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF 522
BLAST of Chy3G065550 vs. NCBI nr
Match:
XP_008444583.1 (PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0060937.1 isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])
HSP 1 Score: 994 bits (2569), Expect = 0.0
Identity = 490/522 (93.87%), Postives = 499/522 (95.59%), Query Frame = 0
Query: 1 MFQFVGLFLHFLDQLLPVFPPIIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNL 60
MF+FVGLFL FLDQLL VFPPIIII LLR L+S NTG+ RLPPSPPGGSFPILGHL L
Sbjct: 1 MFRFVGLFLPFLDQLLLVFPPIIIIF--LLRLLQSKNTGRERLPPSPPGGSFPILGHLYL 60
Query: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
LKNPLHRTLAKLSA+HGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH
Sbjct: 61 LKNPLHRTLAKLSAQHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
Query: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENG 180
LGCNYTNILWAPYGPHWRN RRIATLQILSP R+HMYGSVR EEVKFLIRRLCG GENG
Sbjct: 121 LGCNYTNILWAPYGPHWRNLRRIATLQILSPNRVHMYGSVRREEVKFLIRRLCGGIGENG 180
Query: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP
Sbjct: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
Query: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQ 300
+FLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERR MRRD GDEMKESRVMADVLLDLQQ
Sbjct: 241 SFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRRMRRDNGDEMKESRVMADVLLDLQQ 300
Query: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
TEPHYYTDAFIR MMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL
Sbjct: 301 TEPHYYTDAFIRCMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
Query: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
LQESDLSNLPFL+SVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN
Sbjct: 361 LQESDLSNLPFLHSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
Query: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
D DSWPDPAEF+PERFQD GEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLG LIQCFE
Sbjct: 421 DGDSWPDPAEFRPERFQDVGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGSLIQCFE 480
Query: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPTF 522
WRRVGEEMVDMSEGGGLTMPRA+P WA RPRPILLRLLPTF
Sbjct: 481 WRRVGEEMVDMSEGGGLTMPRAKPCWAKCRPRPILLRLLPTF 520
BLAST of Chy3G065550 vs. NCBI nr
Match:
XP_038884245.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 450/521 (86.37%), Postives = 478/521 (91.75%), Query Frame = 0
Query: 1 MFQFVGLFLHFLDQLLPVFPPIIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNL 60
MFQFV L L FL+ LL FP III FLL FL++ N G+RRLPPSP G SFPILGHL+L
Sbjct: 1 MFQFVELSLPFLNYLL--FPTIII--FLLFHFLQTKNPGRRRLPPSPVGVSFPILGHLHL 60
Query: 61 LKNPLHRTLAKLSAKHGPILLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKH 120
LK PLHRTLAKLS +HGPILLLHFGSRPVLL+SSPSAAQECLTKNDIAFASRPRLSVGKH
Sbjct: 61 LKKPLHRTLAKLSTQHGPILLLHFGSRPVLLVSSPSAAQECLTKNDIAFASRPRLSVGKH 120
Query: 121 LGCNYTNILWAPYGPHWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENG 180
LGCNYT++LWAPYGPHWRNQRRIATLQILSP RI MYGSVRTEEVKFLI RLCG TGENG
Sbjct: 121 LGCNYTSLLWAPYGPHWRNQRRIATLQILSPTRIQMYGSVRTEEVKFLICRLCGGTGENG 180
Query: 181 FRMVEMKKVFFETVLNVMMRMIAGKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLP 240
FR VEMK+VFFETVLNVMM+MI GKRYCGERESEEAERFR+IVKEGF+VSGATNMGDFLP
Sbjct: 181 FRAVEMKRVFFETVLNVMMKMIIGKRYCGERESEEAERFRKIVKEGFEVSGATNMGDFLP 240
Query: 241 NFLKWVGLWSGIEKRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQ 300
NF+KWVGLW GIEKRMERLQGLR+ MQNLIEERR MRRD EMK RVMADVLLDLQ+
Sbjct: 241 NFVKWVGLWDGIEKRMERLQGLRESLMQNLIEERRKMRRDDDGEMKGCRVMADVLLDLQE 300
Query: 301 TEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRL 360
EPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLN+P+SLLKAQ EID VGPNRL
Sbjct: 301 IEPHYYTDAFIRGMMQGMLSAGTDTSSATMEWALSLLLNNPHSLLKAQAEIDAQVGPNRL 360
Query: 361 LQESDLSNLPFLNSVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQN 420
LQESDLSNLPFL+S+LKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLL+NNWAIQN
Sbjct: 361 LQESDLSNLPFLHSILKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLINNWAIQN 420
Query: 421 DSDSWPDPAEFKPERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
D +SWPDP+EF+P+RFQ AGEVEE RWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE
Sbjct: 421 DPESWPDPSEFRPDRFQ-AGEVEELWRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFE 480
Query: 481 WRRVGEEMVDMSEGGGLTMPRARPLWANYRPRPILLRLLPT 521
W+ GEEMVDMSEGGGLTMP+A+PLWAN RPRPILLRLLPT
Sbjct: 481 WQTAGEEMVDMSEGGGLTMPKAKPLWANCRPRPILLRLLPT 516
BLAST of Chy3G065550 vs. NCBI nr
Match:
KAF8377608.1 (hypothetical protein HHK36_030990 [Tetracentron sinense])
HSP 1 Score: 614 bits (1584), Expect = 5.58e-215
Identity = 311/501 (62.08%), Postives = 378/501 (75.45%), Query Frame = 0
Query: 25 IIFLLLRFLRSNNTGQ-RRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHGPILLLH 84
+IFL L L + + + LPPSP S P LGHL LLK PLHRTLA +SA+HGPIL L
Sbjct: 8 LIFLSLFLLTKHVLHKSKNLPPSPL--SLPFLGHLYLLKKPLHRTLATISAQHGPILFLR 67
Query: 85 FGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQRRI 144
FGSRPVLL+SSPSAA+EC TKNDI FA+RPRL GKHLG N+T++ WAPYG HWRN RRI
Sbjct: 68 FGSRPVLLVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNFTSLAWAPYGDHWRNLRRI 127
Query: 145 ATLQILSPARIHMYGSVRTEEVKFLIRRLC-GCTGENGFRMVEMKKVFFETVLNVMMRMI 204
L+I S R+ M+ +R++EV+ +I+RL GC+ + GF+MVEMK VFFE LN+MMRMI
Sbjct: 128 TALEIFSSNRLQMFSGIRSDEVRSMIQRLVRGCSSDGGFKMVEMKSVFFELTLNIMMRMI 187
Query: 205 AGKRYCGER----ESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMER 264
AGKRY GE ESE A +FREIV E F + GATN+GDFLP LKWVG + G+EK + R
Sbjct: 188 AGKRYYGENVGESESEGARKFREIVTETFHLGGATNIGDFLP-VLKWVG-FKGMEKPLMR 247
Query: 265 LQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGM 324
LQ RDGFMQ LIEE R MR K + + + M DVLL LQ+TEP YYTD IRG+M +
Sbjct: 248 LQEKRDGFMQELIEEHRRMR-SKFSDGERKKTMIDVLLSLQETEPEYYTDQIIRGIMLVL 307
Query: 325 LSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLKE 384
LSAGTDTSS TMEWALSLLLN+P L KAQ+EID VG RLL ES+++ LP+L+S++ E
Sbjct: 308 LSAGTDTSSGTMEWALSLLLNNPEVLEKAQDEIDLRVGQERLLDESNMAVLPYLHSIINE 367
Query: 385 TLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQD 444
TLR+YP PLLVPHESS+ C+VGGF+VPRGTMLL N WAI ND W +P +FKPER +
Sbjct: 368 TLRMYPAGPLLVPHESSEGCIVGGFNVPRGTMLLANMWAIHNDPKVWAEPKKFKPERLEG 427
Query: 445 AGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGLT 504
+G +++PFG+GRRGCPGEGLA R+VGL LG LIQCFEW+RVGEE+VDM+EG GLT
Sbjct: 428 LEGRRDGFKFMPFGSGRRGCPGEGLAERVVGLALGSLIQCFEWQRVGEELVDMTEGTGLT 487
Query: 505 MPRARPLWANYRPRPILLRLL 519
+P+A PL A R RP ++ L+
Sbjct: 488 LPKAHPLEAKCRLRPTMVNLI 503
BLAST of Chy3G065550 vs. NCBI nr
Match:
XP_015384205.2 (isoflavone 2'-hydroxylase-like isoform X2 [Citrus sinensis] >XP_024952266.1 isoflavone 2'-hydroxylase-like isoform X1 [Citrus sinensis])
HSP 1 Score: 614 bits (1583), Expect = 7.91e-215
Identity = 313/495 (63.23%), Postives = 381/495 (76.97%), Query Frame = 0
Query: 27 FLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHGPILLLHFGS 86
FL FL+ N R LPPSP S PI+GHL+LLK PLHRTLAKLS ++GPIL + FGS
Sbjct: 17 FLTKYFLQVN----RNLPPSP-AVSLPIIGHLHLLKKPLHRTLAKLSNQYGPILFIRFGS 76
Query: 87 RPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQRRIATL 146
RPV+L+SSPSAA+EC TKNDI FA+RPRL GKHLG NYT ++WAPYG HWRN RRIA+L
Sbjct: 77 RPVVLVSSPSAAEECFTKNDIIFANRPRLLAGKHLGYNYTTLVWAPYGDHWRNLRRIASL 136
Query: 147 QILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVLNVMMRMIAGKR 206
++LS R+ M+ +R +EVK L +RL + +++V+MK +FFE LNV+MRMIAGKR
Sbjct: 137 ELLSSNRLQMFHGIRVDEVKLLCQRLFRGSKSGEYQIVDMKSMFFELTLNVVMRMIAGKR 196
Query: 207 YCGERES--EEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMERLQGLRD 266
Y GE S E+A RF+ IV E F++SGATN+ D LP F+KWVGL S EKR+ L G RD
Sbjct: 197 YYGENVSGSEDARRFKAIVTETFQLSGATNVVDLLP-FVKWVGL-SKTEKRLMILHGKRD 256
Query: 267 GFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGMLSAGTD 326
FMQ+LIEERR +R D E + SR M DVLL LQ+TEP YYTD IRGMMQ +L AG+D
Sbjct: 257 KFMQDLIEERRRLRCDSASEQR-SRTMIDVLLSLQETEPEYYTDHIIRGMMQVLLGAGSD 316
Query: 327 TSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLKETLRIYP 386
TS+ATMEWALS LLN+P L++AQ EIDT+VG +RL++ESDL+ LP+L+ V+KETLR+YP
Sbjct: 317 TSAATMEWALSSLLNNPEILVQAQSEIDTNVGQSRLIEESDLTALPYLHGVIKETLRMYP 376
Query: 387 VAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQDAGEVEE 446
APLLVPHESS++C +GGF VPRGTMLLVN+WAIQND W +P++FKPERF++ +
Sbjct: 377 AAPLLVPHESSEECTIGGFKVPRGTMLLVNSWAIQNDPRIWEEPSKFKPERFREMAGKSD 436
Query: 447 GLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGLTMPRARP 506
LPFG GRR CPGEGLAMR+VGL LG LIQCFEW R G E+VDMSEG GLTMPRA+P
Sbjct: 437 KYMLLPFGTGRRVCPGEGLAMRLVGLALGSLIQCFEWVRTGGELVDMSEGTGLTMPRAQP 496
Query: 507 LWANYRPRPILLRLL 519
L A RPRP ++ LL
Sbjct: 497 LLAMCRPRPAMVTLL 503
BLAST of Chy3G065550 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 458.8 bits (1179), Expect = 6.0e-129
Identity = 241/504 (47.82%), Postives = 323/504 (64.09%), Query Frame = 0
Query: 24 IIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHG--PILL 83
+ I L L F+ + LPPS P + P++GHL LLK PLHR +S G PI+
Sbjct: 11 LFISLSLTFIIGRIKRRPNLPPS-PSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIIS 70
Query: 84 LHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQR 143
L G+R V ++SS S A+EC TKND+ A+R KH+ T ++ A YG HWRN R
Sbjct: 71 LRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLR 130
Query: 144 RIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENG---FRMVEMKKVFFETVLNVM 203
RI ++I S R++ + S+R +E I RL C N F VEMK +F N +
Sbjct: 131 RIGAVEIFSAHRLNSFSSIRRDE----IHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNI 190
Query: 204 MRMIAGKRYCGE--RESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRM 263
+RM+AGK Y G+ + EA+R RE++ EG GA N D+LP L W+ +G EKR+
Sbjct: 191 IRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLP-ILTWI---TGSEKRI 250
Query: 264 ERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQ 323
+++ D F+Q L++ERR +G E K M D LL LQ+T+P YYTD I+G+M
Sbjct: 251 KKIASRLDEFLQGLVDERR-----EGKE-KRQNTMVDHLLCLQETQPEYYTDNIIKGIML 310
Query: 324 GMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVL 383
++ AGTDTS+ T+EW LS LLNHP L KA++EID VG NRL++ESDLS+LP+L +++
Sbjct: 311 SLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIV 370
Query: 384 KETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERF 443
E+LR+YP +PLLVPH +S+DC VGG+H+PRGTMLL N WAI D W DP FKPERF
Sbjct: 371 SESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERF 430
Query: 444 QDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSE-GG 503
+ GE + + L FG GRR CPG GLA R+ LT+G LIQCFEW R+GEE VDM+E GG
Sbjct: 431 EKEGEAQ---KLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGG 490
Query: 504 GLTMPRARPLWANYRPRPILLRLL 520
G+ MP+A PL A + RP++ ++L
Sbjct: 491 GVIMPKAIPLVAMCKARPVVGKIL 496
BLAST of Chy3G065550 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 448.7 bits (1153), Expect = 6.2e-126
Identity = 236/503 (46.92%), Postives = 326/503 (64.81%), Query Frame = 0
Query: 22 IIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHG--PI 81
++ +IFL+ R R N LPPS P + P++GHL LLK PLHR +S G PI
Sbjct: 14 VLSLIFLIGRIKRKLN-----LPPS-PAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPI 73
Query: 82 LLLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRN 141
+ L G+R + ++SS S A+EC TKND+ A+R KH+ + ++ A Y HWRN
Sbjct: 74 ISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRN 133
Query: 142 QRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVLNVMM 201
RRI L+I S R++ + S+R +E++ LI RL GF VEMK +F + N ++
Sbjct: 134 LRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLR-NSSYGFTKVEMKSMFSDLTFNNII 193
Query: 202 RMIAGKRYCGE--RESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRME 261
RM+AGK Y G+ + EA+R R ++ E SG N D++P L W+ + E R++
Sbjct: 194 RMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIP-ILTWI---TYSETRIK 253
Query: 262 RLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQG 321
+L G D F+Q L++E+R +G E KE+ M D LL LQ+T+P YY D I+G M
Sbjct: 254 KLAGRLDEFLQGLVDEKR-----EGKEKKEN-TMVDHLLCLQETQPEYYMDRIIKGTMLS 313
Query: 322 MLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLK 381
+++ GTDT++ T+EWALS LLN+P L KA++EID +G +RLL+ESD+ NLP+L +++
Sbjct: 314 LIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVS 373
Query: 382 ETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQ 441
ETLR+YP AP+L+PH +S+DC VGG+ +PRGTMLL N WAI D W DP FKPERF+
Sbjct: 374 ETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFE 433
Query: 442 DAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGL 501
GE + + +PFG GRR CPG GLA R+V L+LG LIQCFEW R+GEE VDM+EG GL
Sbjct: 434 KEGEAK---KLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGL 493
Query: 502 TMPRARPLWANYRPRPILLRLLP 521
TMP+ARPL A R R + ++LP
Sbjct: 494 TMPKARPLEAMCRARDFVGKILP 496
BLAST of Chy3G065550 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 229/500 (45.80%), Postives = 329/500 (65.80%), Query Frame = 0
Query: 23 IIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKL--SAKHGPIL 82
I+ + L L +L + LPPS P S P++GHL LLK P+HRT L S + PI
Sbjct: 10 ILFVVLSLIYLIGKLKRKPNLPPS-PAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 69
Query: 83 LLHFGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQ 142
L G+R V + SS S A+EC TKND+ A+RP + KH+ +YT ++ A YG HWRN
Sbjct: 70 SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 129
Query: 143 RRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVLNVMMR 202
RRI +++I S R++ + S+R +E++ L+ RL + F V+MK + + N ++R
Sbjct: 130 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQE-FVKVDMKSMLSDLTFNNILR 189
Query: 203 MIAGKRYCGE--RESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMER 262
M+AGKRY G+ + EA+R R+++ + +GA N D+LP + L S E R+++
Sbjct: 190 MVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLP----VLRLVSDYETRVKK 249
Query: 263 LQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGM 322
L G D F+Q L++E+R ++KG+ M D LL LQ+++P Y+TD I+G M +
Sbjct: 250 LAGRLDEFLQGLVDEKRE-AKEKGN------TMIDHLLTLQESQPDYFTDRIIKGNMLAL 309
Query: 323 LSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLKE 382
+ AGTDTS+ T+EWALS +LNHP+ L KA++EID +G +RL+ ESD+SNLP+L +++ E
Sbjct: 310 ILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSE 369
Query: 383 TLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQD 442
TLR+YP AP+L+PH +S+DC V G+ +PRGT+LL N WAI D W DP FKPERF+
Sbjct: 370 TLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK 429
Query: 443 AGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGLT 502
GE + + +PFG GRR CPG GLA R++ LTLG LIQC EW ++GEE VDMSEG G+T
Sbjct: 430 EGEAQ---KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVT 489
Query: 503 MPRARPLWANYRPRPILLRL 519
MP+A+PL A R RP ++++
Sbjct: 490 MPKAKPLEAMCRARPSVVKI 492
BLAST of Chy3G065550 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 439.1 bits (1128), Expect = 4.9e-123
Identity = 233/496 (46.98%), Postives = 319/496 (64.31%), Query Frame = 0
Query: 26 IFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSAKHG--PILLLH 85
+F L FL + + + LPPS P S P++GHL+LLK PLHRT LS G P+ L
Sbjct: 14 LFTLAIFLLTRSKRKLNLPPS-PAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPVFHLR 73
Query: 86 FGSRPVLLISSPSAAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGPHWRNQRRI 145
G+R V +ISS S A+EC TKND+ A+RP+ ++ KHLG N T +L A YG HWRN RRI
Sbjct: 74 LGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRNLRRI 133
Query: 146 ATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVLNVMMRMIA 205
A ++I S R++ + +R +E++ LI L +GF VEMK + N +RM+A
Sbjct: 134 AAVEIFSTHRLNSFLYIRKDEIRRLISHL-SRDSLHGFVEVEMKTLLTNLASNTTIRMLA 193
Query: 206 GKRYCGERESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIEKRMERLQGLR 265
GKRY GE ++++A+ + +V E +GA N D+L + L+WV S EKR++ L
Sbjct: 194 GKRYFGE-DNDDAKLVKNLVSEAVTSAGAGNPIDYL-SILRWV---SSYEKRIKNLGNRF 253
Query: 266 DGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRGMMQGMLSAGT 325
D F+Q L++E+R E ++ M D LL LQ +P YYTD I+G++ ++ AGT
Sbjct: 254 DTFLQKLVDEKRA-------EKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGT 313
Query: 326 DTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLNSVLKETLRIY 385
DTSS T+EWA+S LLNHP L KA+ EID VG +RL+ ESD+ NL +L S++ ETLR+Y
Sbjct: 314 DTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMY 373
Query: 386 PVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKPERFQDAGEVE 445
P PLL+PH SS+DC VGG+ +P GTM+L N WA+ D + W DP FKPERF+ GE E
Sbjct: 374 PAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEGEAE 433
Query: 446 EGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSEGGGLTMPRAR 505
+ + FG GRR CPG GLA R++ LG L+QCFEW RVGE+ VDM+E G T+P+A
Sbjct: 434 ---KLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAI 492
Query: 506 PLWANYRPRPILLRLL 520
PL A + R I+ +L+
Sbjct: 494 PLRAMCKARSIVDKLI 492
BLAST of Chy3G065550 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 436.4 bits (1121), Expect = 3.2e-122
Identity = 235/506 (46.44%), Postives = 329/506 (65.02%), Query Frame = 0
Query: 22 IIIIIFLLLRFLRSNNTGQRRLPPSPPGGSFPILGHLNLLKNPLHRTLAKLSA-----KH 81
I +IFL++ F + ++ LPPSPPG PI+GHL LLK P+HRTL S
Sbjct: 13 IFSLIFLIISF-KFLKPKKQNLPPSPPGW-LPIIGHLRLLKPPIHRTLRSFSETLDHNDG 72
Query: 82 GPILLLHFGSRPVLLISSPS-AAQECLTKNDIAFASRPRLSVGKHLGCNYTNILWAPYGP 141
G ++ L GSR V ++SS AA+EC KND+ A+RP++ +GKH+G N TN++ APYG
Sbjct: 73 GGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGD 132
Query: 142 HWRNQRRIATLQILSPARIHMYGSVRTEEVKFLIRRLCGCTGENGFRMVEMKKVFFETVL 201
HWRN RR+ T++I S R++ + VRT+EV+ LI RL G +VE+K + +
Sbjct: 133 HWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKK-TVVELKPMLMDLTF 192
Query: 202 NVMMRMIAGKRYCGER--ESEEAERFREIVKEGFKVSGATNMGDFLPNFLKWVGLWSGIE 261
N +MRM+ GKRY GE + EEA+R R++V + + + N D++P + L+S E
Sbjct: 193 NNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVP----ILRLFSSYE 252
Query: 262 KRMERLQGLRDGFMQNLIEERRTMRRDKGDEMKESRVMADVLLDLQQTEPHYYTDAFIRG 321
R+++L D F+Q LI+ DK + + M D LL LQ+++ YYTD I+G
Sbjct: 253 NRVKKLGEETDKFLQGLID-------DKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKG 312
Query: 322 MMQGMLSAGTDTSSATMEWALSLLLNHPNSLLKAQEEIDTHVGPNRLLQESDLSNLPFLN 381
++ M+ AGT+TS+ T+EWALS LLNHP+ + KA++EID VG +RL++E+DLS LP+L
Sbjct: 313 IILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLK 372
Query: 382 SVLKETLRIYPVAPLLVPHESSQDCVVGGFHVPRGTMLLVNNWAIQNDSDSWPDPAEFKP 441
+++ ETLR++P PLLVPH +S+DC +G + +PRGT LLVN WAI D ++W DP FKP
Sbjct: 373 NIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKP 432
Query: 442 ERFQDAGEVEEGLRWLPFGAGRRGCPGEGLAMRMVGLTLGCLIQCFEWRRVGEEMVDMSE 501
ERF+ + EE + L FG GRR CPG GLA R+VGL LG LIQCFEW RVG VDM E
Sbjct: 433 ERFE---KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKE 492
Query: 502 GGGLTMPRARPLWANYRPRPILLRLL 520
G G T+P+A PL A + RP L +++
Sbjct: 493 GVGNTVPKAIPLKAICKARPFLHKII 501
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| W8JMU7 | 2.7e-134 | 49.31 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
| Q6WNQ8 | 1.8e-125 | 47.48 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
| Q6WNQ9 | 5.7e-124 | 47.99 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
| Q9FG65 | 4.5e-121 | 46.44 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
| Q9LHA1 | 3.8e-120 | 46.22 | Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LR62 | 2.4e-303 | 98.47 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354920 PE=3 SV=1 | [more] |
| A0A5A7V256 | 1.2e-286 | 93.87 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... | [more] |
| A0A1S3BBE3 | 1.2e-286 | 93.87 | isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103487855 PE=3 SV=1 | [more] |
| A0A5B6WTN2 | 5.8e-172 | 61.00 | Isoflavone 2'-hydroxylase-like OS=Gossypium australe OX=47621 GN=EPI10_006814 PE... | [more] |
| V4T5X6 | 9.9e-172 | 63.23 | Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10000918mg PE=3 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_004143022.1 | 0.0 | 98.47 | cytochrome P450 81Q32 [Cucumis sativus] >KGN62466.1 hypothetical protein Csa_018... | [more] |
| XP_008444583.1 | 0.0 | 93.87 | PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0060937.1 isoflavon... | [more] |
| XP_038884245.1 | 0.0 | 86.37 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
| KAF8377608.1 | 5.58e-215 | 62.08 | hypothetical protein HHK36_030990 [Tetracentron sinense] | [more] |
| XP_015384205.2 | 7.91e-215 | 63.23 | isoflavone 2'-hydroxylase-like isoform X2 [Citrus sinensis] >XP_024952266.1 isof... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G37340.1 | 6.0e-129 | 47.82 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
| AT4G37360.1 | 6.2e-126 | 46.92 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
| AT4G37370.1 | 2.6e-124 | 45.80 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT4G37330.1 | 4.9e-123 | 46.98 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
| AT5G36220.1 | 3.2e-122 | 46.44 | cytochrome p450 81d1 | [more] |
