Chy3G065190 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G065190
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Calmodulin
Location	chrH03: 17584568 .. 17589145 (-)
RNA-Seq Expression	Chy3G065190
Synteny	Chy3G065190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAACTCCAAAAGCAAAACTTACAAACGAGTTCTAGTTCCATTCTCAGAGGAGCAACTGGCCAATGTTTTCAAGAACCATGACCGAGACGGGGATGGGAATCTGACGAAGGAGGAGTTGAAGCAAGCCTTCGAATATCTCGGCTCACGCTTCTGCAACTTTCGGGTAGAAGAAGCCCTACGTGCCGCCGACACCAACGGGGACGGTGTCATCAGCATGGATGAGATGGGGAAGCTAATTAAGTATGCCAAAACTCGTAAATACACTATTAGTTAAAAAGTAAATGTATGTTAGCTATATCAACTTTAGTTGTTTTTGTTCACCTATATATATATATTCTATATATAAATGTCTTTCATATATTTATCTGTATTTGAATGAGTGGATTCGAATTAATACCCCTCTTTGTCCAATGATATATAAATAAAAAAAAATTGTTAAATGGTTTTGTTGATTGTGTATTGATTCGATCTCACTCTCTGCTTGTCTCTAATATAAAGAATGGACCTAATTTAATTTGCAGGGAGATGGAATAATAATATCATGATTTGATTTGAACTCATGATTCCATTTACGTCGTGTTTGGAATAATTTTTAAATTTTTTTTCTCTATAAGTTGGTTCTGGAAAAATTAATAATTTAGAGTGTATTTAACATAGTCTTGAATTATTTGAGATGATACTAAAATCTTCTTTAAGAAATTATTAATAAATGAAAGTAGGTTAAAGAATTTGGATTCATTTAAAATCATCTTTCAATGATCTAGCTGATGCTTTATCTATAATATAGTTTTTTTTAACTCAATTACCATTTTTTTTTAATTATTTTTATTCAAAAATATCACAAGTATGAGCAACGTACGATATTAATCCATTATATTCTAAACAAAATCGAATTGATCAATTTAAGTTATTTTTGGCAATTTGAAGTTCAAAATCTCTCTCATGTATAATAACAAGATTGCATCGTGTATATATACAAACACTTCTATAAATTTTCAAGGTTTTTTTTAGCACAATAAAGCTAGGATAAATTTTGGTCTTCTTTATTGTTAACACATTCATCATTTTGAACTTAAAAATAGAATGAAATAAAAATCTTGCCAACCATCTAAAAATCCTCCAATAGATTCCATTCTTTTACTATATCAATAGAGTTGCATAGAACACGTAGTTACGTTCTCACATTTTGTCACCAAGCTTTAGACTAGAATGTTTCTTTTCATAGAACATTTAGAATTAAATGACTTTTCACTTCTAGTTAAGATAGAAGATCATACCAATTATTATAATTATACTAAACTTGTTTGGACTACCAACTAGAACATTATGGTCACTCTTTTCGAATAAAACGCAAAACCATAGTCATGCATTTGCTTATACCCTCACATGAAAGAATATTGTTTGTTTTTTTTTTTTTTTTAATTCTTTTTGGTTAAGCTTCTCATTAGGTGTTTTTAATATTACAATAATAAAAAATAAAATAAAAAGGAAGTTGGTCAAATTTTGGAATGAAATTCGTGAAAAACTGATTGGTACGGTCTCCCCTATCTTTGGGGCACTTCCAATTTCTAAATACACCCAATAAACTATTAACACACCCACATATATATATATATAATACTAATCCAAAATCATTTATAACTATAACAAAATTTTTAAATACAGTCAAAATCTATCGCTATTTAAAATTTAACAATAATAAAATTTATGAATAATAGATTTTACTAGATGATTTTTAAATTTCATCATTTCGTAACGTATATGTATATATATTAGTAACTCTAATCGTCATCATCAAATGCATCTATGTGAACGACTTGAGTTAGCTCAATTGATTAATACATTATAATTATATTCTTGCTTGAAATGTCAAAGCTTCTATTTTCTCCCTTTACAACTGTGTCCATGTAAGCAATGGAATCCAATTACATCGAATGCCCATCTGTATTTACTTCTCTATTTAGAATTACTTTCCTTTCCCATTTAATATATCTAATAAATTACTTATTGATTATTAGAATGCAAACTATACCCATATACCCCAATTGTGAGCATCTCAACAAGAAAAATTCTTTCAACAATGAATTGTTTTTTTTTAATCAAATATTTTGAAGATAAAGTGGTATTTTATAAAAAGAAACTTAATTTTGAGCATTTTTTATTGGTAAGATTAGGTAGATATATATAAAGATATTTATTTATCAACTCTTATTTATCAATTTTAATTAATAATCAGAAAGTTGGTAAAAACTTTTTCTGTGGGTTTCTCTCGGAATGTTTTAGAGAGATAGATAGGGTTTAGAGAAAGAATATGGAGATTTGTATTAGAGTTTACAATCATGATTATAACAAAGATATGACTAAGCTTAAATATTAAAGTCTAAGGTCCAATGGACTTGTATATGTAAAATAATAAACTAATACTAATTGTTAATTAACATCAATTAGCATAATATAAACCTTAATACACATCAAAACTACCTACTTTTAACCCTTTCAAGTATGTATTATTGTGATTCTTTTTTAACTTAATTTAGCCTCCACTTTAATATTATTTTGTTTTTATAAACTAAATTTATAAACACTACTTGTCTATAGGTTTTTATATTTTCTAATTAACTTTCTTCCATAAACAAATACAAAAACCAAGTTTTAGAAACTATTTAAATATTTATTTTTGTTTTTAGAATTGAACATTAAAAATTTACCATAAAAAAGAGAGAAAACAAACTCATTTTTCTTCTTTTTTTCTTTTTAAGATAAAATAAAATAGCTATGAAATGAAGCACATTACTTTTGTTGTTGTGTGTTCTTTAATGTCAAGATAAATATATAATTTTACACCTATATAGGTTTGATGGAAAATTATCCTAAAGATTACTTGATTTTTTTTTATATAAAATATAGGAAACTAAAAAAGTCCCATAAAAAACTTATTTTGAATTTTTTTAAAACATAAAACAAAAAACGATTGCCAAACATCTATTTTTTACTTTTGGTCTTTAAAAAACAAAAAACTAAGAACACCAACCAAATAAGTAAAGTTTTTGTTTTTACTGTTAATCATTTCTTGAATATATTTTAGAATTTTGAATGATTTTGAGTTAATCTTGACCATCTATCTCACACGAGGACAATAGAAAATATAAATTTACTGTACATATCAATTTGTAAATACAAGCTATTAAGGTTAAACATGTTAACCACAAACGATTATTTTTATGTATCTCTACGTATCACTATAATACTATGATACATATTTTTGAGTTTACACCTTTGTTTTTTATTTCATAAAAAATTTATACCAATAAATTAAAAATAATTGTCCCATTTCAATTTTCAAAGACGTTGTATTTTCTCTACTGCGCCATGGACACTACCCAGACAGTTTGTATTATGTTTGGTGTGTATTCAACATAACAACTTTCCCTATTAAGTCAGGCCCTACTTTTAAAGAGAATATGCTTTCCATCAAATGTTGAAATTAATCTTCGTATAAAATGGGCCCTTTGGGAATTAAATTTGTCAGCTACCAACTTTCTGACACAGAGGAAAATAAAACTTATTATTTACAAAAGTAGTCTCTCGTTAATGATCTAGTGCATGCAAACTTGAGTATTATGTATTTCGAAGAAAGATAAATGATAAAAACTACACCTTTTAAATATGATGATAGTTGTAAATGTATACCTTCAAATTTTTAATTGAAAAATGAAATGTTAAAATTGAACACTTAAACTCATTACATAACTGTAGGAATGGTAACAATTGTTTGCTATGTCTGAAGAATCAATTTTTATCATTTAAGGTCCTACTCTTAATAGAAGAAACAAAACATCTTTGAAGATGGTGAGTCCACCTGTGCATTATTTTACATGTTTATATAGATAGTTCTTAATATAAAAAGAAAAATTGAAATGTAAGAACTAAATTTATATATATAAAGAAAAAAAACATGTTTCTAAAAAGTTTTAAATTACTAGAAATTAGGTGGGCTAAAAATTCCACTTTTTTTTATCTTCCTTCATAGGTCAAGAAAAAGGGAAACCAACAATTCTCCCTCTCTTCTTCATAACAGAAGAAACACATGTCCAAAAAAATTGAGATTTGGTAGGCTGCTTGCTTCATGAAGATGGTGAGTCCAAACGTGTGAGAAAGAAACACCGCACGTTCATTTCACATGTTTATAGATATAAATCTCTCCCATATTTACCTCCCATTGTAAATTAAACCAACACAAAAGTCTTTCCCTCCTCTTACCTGTTCTTCTTTTAGAGCAAAACCCTTATACACATATTATAACCCATCCATGGGGACCTCTAACGGCTTGATTTTTAAGTGTTTTCCAGCGTCGTTGACGGAGTTTGAGGTGGCCAAAGTTTTCAAGAACCACGACAAGGATGGAGATGGAAGGCTGACCAAGAAAGAGCTGAAGAGCGCCTTCAAGTACTTGGGTTACAAGTTCACCACCTTTAGAGTCCACCAAGCCCTCCACGCCGCTGACGACAACTGCGACGATGTCATCAGCTTTGAGGAGATGAGTAAACTTATTATGTATACCGCAGGTTCATAG

mRNA sequence

ATGGGGAACTCCAAAAGCAAAACTTACAAACGAGTTCTAGTTCCATTCTCAGAGGAGCAACTGGCCAATGTTTTCAAGAACCATGACCGAGACGGGGATGGGAATCTGACGAAGGAGGAGTTGAAGCAAGCCTTCGAATATCTCGGCTCACGCTTCTGCAACTTTCGGGTAGAAGAAGCCCTACGTGCCGCCGACACCAACGGGGACGGTGTCATCAGCATGGATGAGATGGGGAAGCTAATTAAGTATGCCAAAACTCCGTCGTTGACGGAGTTTGAGGTGGCCAAAGTTTTCAAGAACCACGACAAGGATGGAGATGGAAGGCTGACCAAGAAAGAGCTGAAGAGCGCCTTCAAGTACTTGGGTTACAAGTTCACCACCTTTAGAGTCCACCAAGCCCTCCACGCCGCTGACGACAACTGCGACGATGTCATCAGCTTTGAGGAGATGAGTAAACTTATTATGTATACCGCAGGTTCATAG

Coding sequence (CDS)

ATGGGGAACTCCAAAAGCAAAACTTACAAACGAGTTCTAGTTCCATTCTCAGAGGAGCAACTGGCCAATGTTTTCAAGAACCATGACCGAGACGGGGATGGGAATCTGACGAAGGAGGAGTTGAAGCAAGCCTTCGAATATCTCGGCTCACGCTTCTGCAACTTTCGGGTAGAAGAAGCCCTACGTGCCGCCGACACCAACGGGGACGGTGTCATCAGCATGGATGAGATGGGGAAGCTAATTAAGTATGCCAAAACTCCGTCGTTGACGGAGTTTGAGGTGGCCAAAGTTTTCAAGAACCACGACAAGGATGGAGATGGAAGGCTGACCAAGAAAGAGCTGAAGAGCGCCTTCAAGTACTTGGGTTACAAGTTCACCACCTTTAGAGTCCACCAAGCCCTCCACGCCGCTGACGACAACTGCGACGATGTCATCAGCTTTGAGGAGATGAGTAAACTTATTATGTATACCGCAGGTTCATAG

Protein sequence

MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDEMGKLIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS*

Homology

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BLAST of Chy3G065190 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-15
Identity = 50/147 (34.01%), Postives = 75/147 (51.02%), Query Frame = 0


Query: 15  PFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISM 74
           P  EE++  VF+  D +GDG +++ EL   FE LG    +  +   +  AD +GDG IS+
Sbjct: 35  PTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISL 94

Query: 75  DEMGKLIKYAK-TPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQA 134
           DE   L   A    +  E ++   F+  D DG+G ++  EL      LG K T  +  + 
Sbjct: 95  DEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRM 154

Query: 135 LHAADDNCDDVISFEEMSKLIMYTAGS 161
           +   D N D +ISFEE  K++M   GS
Sbjct: 155 IEGVDQNGDGLISFEEF-KVMMAGGGS 180

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BLAST of Chy3G065190 vs. ExPASy Swiss-Prot
Match: Q9ZSA4 (Calcium-dependent protein kinase 27 OS=Arabidopsis thaliana OX=3702 GN=CPK27 PE=1 SV=3)

HSP 1 Score: 79.7 bits (195), Expect = 3.3e-14
Identity = 55/154 (35.71%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 9   YKRVLVPF-----SEEQ---LANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 68
           +K+V++ F     SEE+   L  +F N D D  GN+T EELK     LGS      VE+ 
Sbjct: 317 FKKVVLKFIAANLSEEEIKGLKTLFTNIDTDKSGNITLEELKTGLTRLGSNLSKTEVEQL 376

Query: 69  LRAADTNGDGVISMDEMGKLIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKY 128
           + AAD +G+G I +DE      +       E  V K F++ DKD DG +TK+EL+ A K 
Sbjct: 377 MEAADMDGNGTIDIDEFISATMHRYKLDRDE-HVYKAFQHFDKDNDGHITKEELEMAMKE 436

Query: 129 LGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 155
            G       + Q +  AD + D  I+FEE   ++
Sbjct: 437 DG-AGDEGSIKQIIADADTDNDGKINFEEFRTMM 468

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BLAST of Chy3G065190 vs. ExPASy Swiss-Prot
Match: P14533 (Squidulin OS=Doryteuthis pealeii OX=1051067 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 7.3e-14
Identity = 44/142 (30.99%), Postives = 78/142 (54.93%), Query Frame = 0


Query: 17  SEEQLANV---FKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVIS 76
           SE+Q+A +   F   D DGDG +T +EL+   + LG    +  +EE +R  DT+G+G I 
Sbjct: 5   SEKQIAEIKDAFDMFDIDGDGQITSKELRSVMKSLGRTPSDAELEEMIREVDTDGNGTIE 64

Query: 77  MDEMGKLIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAF-KYLGYKFTTFRVHQ 136
             E  +++     P+  E E+ + F+  DKDG+G +T  EL+     +   K T+  + +
Sbjct: 65  YAEFVEMMAKQMGPTDPEKEMREAFRVFDKDGNGLITAAELRQVMANFSDEKLTSEEISE 124

Query: 137 ALHAADDNCDDVISFEEMSKLI 155
            +  AD + D ++++EE  K++
Sbjct: 125 MIREADIDGDGMVNYEEFVKMM 146

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BLAST of Chy3G065190 vs. ExPASy Swiss-Prot
Match: A8I1Q0 (Calmodulin OS=Heterocapsa triquetra OX=66468 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 9.6e-14
Identity = 45/142 (31.69%), Postives = 73/142 (51.41%), Query Frame = 0


Query: 17  SEEQLA---NVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVIS 76
           +EEQ+A     F   D+DGDG +T +EL      LG       +++ +   D++G+G I 
Sbjct: 6   TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDSDGNGTID 65

Query: 77  MDEMGKLIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQA 136
             E   L+      + TE E+ + FK  D+DG+G ++  EL+     LG K T   V + 
Sbjct: 66  FPEFLSLMARKMKDTDTEEELIEAFKVFDRDGNGFISAAELRHVMTNLGEKLTDEEVDEM 125

Query: 137 LHAADDNCDDVISFEEMSKLIM 156
           +  AD + D  I++EE  K++M
Sbjct: 126 IREADVDGDGQINYEEFVKMMM 147

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BLAST of Chy3G065190 vs. ExPASy Swiss-Prot
Match: P84339 (Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 44/142 (30.99%), Postives = 72/142 (50.70%), Query Frame = 0


Query: 17  SEEQLA---NVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVIS 76
           SEEQ++     F   D+DGDG +T +EL      LG       +E+ +   D +G+G I 
Sbjct: 6   SEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPSQAELEDMINEVDADGNGTID 65

Query: 77  MDEMGKLIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQA 136
             E   ++      + +E E+ + FK  DKDG+G ++  EL+     LG K T   V + 
Sbjct: 66  FPEFLTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAAELRHVMTNLGEKLTDSEVDEM 125

Query: 137 LHAADDNCDDVISFEEMSKLIM 156
           +  AD + D  I++EE  K+++
Sbjct: 126 IREADVDGDGQINYEEFVKMML 147

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BLAST of Chy3G065190 vs. ExPASy TrEMBL
Match: A0A0A0LRB6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 2.1e-40
Identity = 86/90 (95.56%), Postives = 87/90 (96.67%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDG+LTKEELKQAFEYLGSRFCNFRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIKYAKTPSLT 91
          LRAADTNGDGVISMDEMGKLIKYAKT   T
Sbjct: 61 LRAADTNGDGVISMDEMGKLIKYAKTRKYT 90

[Close]

BLAST of Chy3G065190 vs. ExPASy TrEMBL
Match: A0A5A7V5B0 (Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00320 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 8.1e-40
Identity = 86/90 (95.56%), Postives = 86/90 (95.56%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRF NFRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFSNFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIKYAKTPSLT 91
          LRAADTNGDGVISMDEMGKLIKYAKT   T
Sbjct: 61 LRAADTNGDGVISMDEMGKLIKYAKTRKYT 90

[Close]

BLAST of Chy3G065190 vs. ExPASy TrEMBL
Match: A0A0A0LP43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 5.6e-33
Identity = 72/73 (98.63%), Postives = 73/73 (100.00%), Query Frame = 0


Query: 88  SLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISF 147
           SLTEFEVAKVFKNHDKDGDGRLTK+ELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISF
Sbjct: 15  SLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISF 74

Query: 148 EEMSKLIMYTAGS 161
           EEMSKLIMYTAGS
Sbjct: 75  EEMSKLIMYTAGS 87

[Close]

BLAST of Chy3G065190 vs. ExPASy TrEMBL
Match: A0A7N2KRS3 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 4.0e-31
Identity = 72/163 (44.17%), Postives = 105/163 (64.42%), Query Frame = 0


Query: 5   KSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAA 64
           K   YK   V ++++QL NVFK++D DGDG L++EELK+AF+YLGSR+ ++R ++ALR  
Sbjct: 150 KVTVYKPTSVLYTKDQLMNVFKSYDADGDGKLSREELKKAFKYLGSRWGSYRADQALRCT 209

Query: 65  DTNGDGVISMDEMGKLIKYA-----------KTPSLTEFEVAKVFKNHDKDGDGRLTKKE 124
           D N DG I   E+ KL+ Y             +    E ++  +FK+HD DGDG+L++ E
Sbjct: 210 DANDDGYIDEKELEKLVDYTLERRKAMAFKRTSVPFNEQQLLNIFKSHDVDGDGKLSRDE 269

Query: 125 LKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMY 157
           LK+AFKYLG ++  +R  QAL  AD N D  I+ +E+ KL+ Y
Sbjct: 270 LKNAFKYLGSRWDAYRTDQALRQADANDDGYITEDELKKLVEY 312

[Close]

BLAST of Chy3G065190 vs. ExPASy TrEMBL
Match: A0A6A6MBN7 (Uncharacterized protein OS=Hevea brasiliensis OX=3981 GN=GH714_005320 PE=4 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.4e-28
Identity = 74/165 (44.85%), Postives = 102/165 (61.82%), Query Frame = 0


Query: 10  KRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGD 69
           K V   ++E QL   FK  D +GDG L+KEE+K+AF+ LGSR   +RV  AL  AD NGD
Sbjct: 7   KSVSGSYTEGQLMAFFKEQDVNGDGRLSKEEIKKAFQQLGSRLPGWRVRRALHHADVNGD 66

Query: 70  GVISMDEMGKLIKY---------AKTP---------SLTEFEVAKVFKNHDKDGDGRLTK 129
           G IS+DE+ +L+KY         AK P         + TE ++  +FK HD +GDGRL+K
Sbjct: 67  GSISLDELDELVKYVESEPHSAVAKMPLSVPKSVSGTYTEGQLKAIFKEHDANGDGRLSK 126

Query: 130 KELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMY 157
           +E+K AF+ LG +   +RV +ALH AD N D  IS +E+ +L+ Y
Sbjct: 127 EEMKKAFQQLGSRLPGWRVRRALHHADANGDGSISVDELDELVKY 171

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BLAST of Chy3G065190 vs. NCBI nr
Match: KGN62765.2 (hypothetical protein Csa_022396 [Cucumis sativus])

HSP 1 Score: 296 bits (757), Expect = 2.71e-100
Identity = 153/180 (85.00%), Postives = 155/180 (86.11%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 60
           MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDG+LTKEELKQAFEYLGSRFCNFRVEEA
Sbjct: 1   MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEA 60

Query: 61  LRAADTNGDGVISMDEMGKLIKYAKT--------------------PSLTEFEVAKVFKN 120
           LRAADTNGDGVISMDEMGKLIK                         SLTEFEVAKVFKN
Sbjct: 61  LRAADTNGDGVISMDEMGKLIKAKPLYTYYNPSMGTSNGLIFKCFPASLTEFEVAKVFKN 120

Query: 121 HDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS 160
           HDKDGDGRLTK+ELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS
Sbjct: 121 HDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAGS 180

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BLAST of Chy3G065190 vs. NCBI nr
Match: KAG7020566.1 (hypothetical protein SDJN02_17252, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 218 bits (556), Expect = 2.18e-68
Identity = 110/161 (68.32%), Postives = 130/161 (80.75%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 60
           MGN+KS  YKRVLVPF+EEQLA +FK+HDRDGDG L+KEELKQAF YLGSRF +FRV+EA
Sbjct: 36  MGNAKSTNYKRVLVPFTEEQLAAIFKSHDRDGDGKLSKEELKQAFNYLGSRFSSFRVKEA 95

Query: 61  LRAADTNGDGVISMDEMGKLIKYAK--TPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAF 120
           LRAADTNGDG ISMDEM KLIKY K     LTE ++A +FK+HD+DGDG+LTK+ELK AF
Sbjct: 96  LRAADTNGDGFISMDEMNKLIKYTKGLLVPLTEEQLAGIFKSHDRDGDGKLTKEELKQAF 155

Query: 121 KYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLIMYTAG 159
            YLG +F++FR  +AL AAD + D  I+  EMSKLI YT  
Sbjct: 156 NYLGSRFSSFRADEALRAADADGDGTINMAEMSKLIQYTKN 196

[Close]

BLAST of Chy3G065190 vs. NCBI nr
Match: KAA0060899.1 (putative Calcium-binding EF-hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 172 bits (437), Expect = 8.45e-53
Identity = 86/90 (95.56%), Postives = 86/90 (95.56%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRF NFRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFSNFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIKYAKTPSLT 90
          LRAADTNGDGVISMDEMGKLIKYAKT   T
Sbjct: 61 LRAADTNGDGVISMDEMGKLIKYAKTRKYT 90

[Close]

BLAST of Chy3G065190 vs. NCBI nr
Match: KAG6598376.1 (hypothetical protein SDJN03_08154, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 152 bits (385), Expect = 6.91e-45
Identity = 76/90 (84.44%), Postives = 82/90 (91.11%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLA+VFKNHD DGDG LTKEELKQAF+YLGSRF +FRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIKYAKTPSLT 90
          LRAADT+GDG ISM EMGKLI+YAK+   T
Sbjct: 61 LRAADTDGDGFISMAEMGKLIQYAKSRKYT 90

[Close]

BLAST of Chy3G065190 vs. NCBI nr
Match: KAG7029343.1 (Calcium-binding protein CML37, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 147 bits (372), Expect = 6.55e-43
Identity = 74/90 (82.22%), Postives = 80/90 (88.89%), Query Frame = 0


Query: 1  MGNSKSKTYKRVLVPFSEEQLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 60
          MGNSKSKTYKRVLVPFSEEQLA+VFKNHD DGDG LTKEELKQAF+YLGSRF +FRVEEA
Sbjct: 1  MGNSKSKTYKRVLVPFSEEQLASVFKNHDGDGDGKLTKEELKQAFDYLGSRFSSFRVEEA 60

Query: 61 LRAADTNGDGVISMDEMGKLIKYAKTPSLT 90
          LRAAD +GDG ISM EM KLI+YAK+   T
Sbjct: 61 LRAADIDGDGFISMAEMDKLIQYAKSRKYT 90

[Close]

BLAST of Chy3G065190 vs. TAIR 10
Match: AT4G04700.1 (calcium-dependent protein kinase 27 )

HSP 1 Score: 79.7 bits (195), Expect = 2.3e-15
Identity = 55/154 (35.71%), Postives = 79/154 (51.30%), Query Frame = 0


Query: 9   YKRVLVPF-----SEEQ---LANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEA 68
           +K+V++ F     SEE+   L  +F N D D  GN+T EELK     LGS      VE+ 
Sbjct: 317 FKKVVLKFIAANLSEEEIKGLKTLFTNIDTDKSGNITLEELKTGLTRLGSNLSKTEVEQL 376

Query: 69  LRAADTNGDGVISMDEMGKLIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKY 128
           + AAD +G+G I +DE      +       E  V K F++ DKD DG +TK+EL+ A K 
Sbjct: 377 MEAADMDGNGTIDIDEFISATMHRYKLDRDE-HVYKAFQHFDKDNDGHITKEELEMAMKE 436

Query: 129 LGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 155
            G       + Q +  AD + D  I+FEE   ++
Sbjct: 437 DG-AGDEGSIKQIIADADTDNDGKINFEEFRTMM 468

[Close]

BLAST of Chy3G065190 vs. TAIR 10
Match: AT4G04695.1 (calcium-dependent protein kinase 31 )

HSP 1 Score: 75.9 bits (185), Expect = 3.4e-14
Identity = 51/157 (32.48%), Postives = 74/157 (47.13%), Query Frame = 0


Query: 1   MGNSKSKTYKRVLVPFSEEQ---LANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRV 60
           M   K    K +    SEE+   L  +F N D D  G +T EELK     LGS      V
Sbjct: 314 MNKLKKVALKVIAANLSEEEIKGLKTLFTNIDTDKSGTITLEELKTGLTRLGSNLSKTEV 373

Query: 61  EEALRAADTNGDGVISMDEMGKLIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSA 120
           E+ + AAD +G+G I +DE      +       +  V + F++ DKD DG +TK+EL+ A
Sbjct: 374 EQLMEAADVDGNGTIDIDEFISATMHRYRLDRDD-HVYQAFQHFDKDNDGHITKEELEMA 433

Query: 121 FKYLGYKFTTFRVHQALHAADDNCDDVISFEEMSKLI 155
            K  G       + Q +   D + D  I+FEE   ++
Sbjct: 434 MKEHGVG-DEVSIKQIITEVDTDNDGKINFEEFRTMM 468

[Close]

BLAST of Chy3G065190 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 75.5 bits (184), Expect = 4.4e-14
Identity = 40/137 (29.20%), Postives = 73/137 (53.28%), Query Frame = 0


Query: 20  QLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDEMGK 79
           +L+ VF+  D++GDG + K ELK  F+ +G       + E +   D NGDG + +DE G 
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 80  LIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYK--FTTFRVHQALHAA 139
           L +        E ++ + F+  D++GDG +T +EL+S    +G K   T     + +   
Sbjct: 65  LYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKV 124

Query: 140 DDNCDDVISFEEMSKLI 155
           D + D +++F+E  +++
Sbjct: 125 DVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Chy3G065190 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 72.0 bits (175), Expect = 4.9e-13
Identity = 42/135 (31.11%), Postives = 72/135 (53.33%), Query Frame = 0


Query: 20  QLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDEMGK 79
           +L  VF   D + DG ++ EEL+++F  LG +  +     A+R +DT+GDG++  +E  +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 80  LIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQALHAADD 139
           LIK        + E+   F+ +  +G+  +T + LK   K LG   TT      + A D 
Sbjct: 103 LIK-VDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDL 162

Query: 140 NCDDVISFEEMSKLI 155
           N D V+SF+E + ++
Sbjct: 163 NADGVLSFDEFALMM 176

[Close]

BLAST of Chy3G065190 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 72.0 bits (175), Expect = 4.9e-13
Identity = 42/135 (31.11%), Postives = 72/135 (53.33%), Query Frame = 0


Query: 20  QLANVFKNHDRDGDGNLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDEMGK 79
           +L  VF   D + DG ++ EEL+++F  LG +  +     A+R +DT+GDG++  +E  +
Sbjct: 43  ELEAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQ 102

Query: 80  LIKYAKTPSLTEFEVAKVFKNHDKDGDGRLTKKELKSAFKYLGYKFTTFRVHQALHAADD 139
           LIK        + E+   F+ +  +G+  +T + LK   K LG   TT      + A D 
Sbjct: 103 LIK-VDDEEEKKMELKGAFRLYIAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDL 162

Query: 140 NCDDVISFEEMSKLI 155
           N D V+SF+E + ++
Sbjct: 163 NADGVLSFDEFALMM 176

The following BLAST results are available for this feature:

BLAST of Chy3G065190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	1.3e-15	34.01	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9ZSA4	3.3e-14	35.71	Calcium-dependent protein kinase 27 OS=Arabidopsis thaliana OX=3702 GN=CPK27 PE=...	[more]
P14533	7.3e-14	30.99	Squidulin OS=Doryteuthis pealeii OX=1051067 PE=1 SV=1	[more]
A8I1Q0	9.6e-14	31.69	Calmodulin OS=Heterocapsa triquetra OX=66468 PE=2 SV=1	[more]
P84339	1.3e-13	30.99	Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2	[more]

BLAST of Chy3G065190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRB6	2.1e-40	95.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357340 PE=4 SV=1	[more]
A0A5A7V5B0	8.1e-40	95.56	Putative Calcium-binding EF-hand family protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A0A0LP43	5.6e-33	98.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G357350 PE=4 SV=1	[more]
A0A7N2KRS3	4.0e-31	44.17	Uncharacterized protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
A0A6A6MBN7	2.4e-28	44.85	Uncharacterized protein OS=Hevea brasiliensis OX=3981 GN=GH714_005320 PE=4 SV=1	[more]

BLAST of Chy3G065190 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN62765.2	2.71e-100	85.00	hypothetical protein Csa_022396 [Cucumis sativus]	[more]
KAG7020566.1	2.18e-68	68.32	hypothetical protein SDJN02_17252, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAA0060899.1	8.45e-53	95.56	putative Calcium-binding EF-hand family protein [Cucumis melo var. makuwa]	[more]
KAG6598376.1	6.91e-45	84.44	hypothetical protein SDJN03_08154, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7029343.1	6.55e-43	82.22	Calcium-binding protein CML37, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]

BLAST of Chy3G065190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G04700.1	2.3e-15	35.71	calcium-dependent protein kinase 27	[more]
AT4G04695.1	3.4e-14	32.48	calcium-dependent protein kinase 31	[more]
AT4G12860.1	4.4e-14	29.20	EF hand calcium-binding protein family	[more]
AT1G76650.1	4.9e-13	31.11	calmodulin-like 38	[more]
AT1G76650.3	4.9e-13	31.11	calmodulin-like 38	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 129..157 e-value: 17.0 score: 7.9 coord: 20..48 e-value: 0.14 score: 21.2 coord: 56..84 e-value: 0.12 score: 21.4 coord: 93..121 e-value: 0.0041 score: 26.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 18..80 e-value: 3.9E-12 score: 46.4 coord: 95..154 e-value: 7.9E-10 score: 39.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 16..51 score: 15.350267
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 52..87 score: 11.249643
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 89..124 score: 15.071314
None	No IPR available	GENE3D	1.10.238.10		coord: 3..82 e-value: 5.6E-15 score: 57.7
None	No IPR available	GENE3D	1.10.238.10		coord: 83..158 e-value: 3.9E-14 score: 55.0
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 88..156 coord: 10..84
None	No IPR available	PANTHER	PTHR10891:SF911	SUBFAMILY NOT NAMED	coord: 88..156 coord: 10..84
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 102..114
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 65..77
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 138..150
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 29..41
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 16..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G065190.1	Chy3G065190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding