Chy3G065170 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G065170
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	EF hand calcium-binding protein family
Location	chrH03: 17565501 .. 17571117 (-)
RNA-Seq Expression	Chy3G065170
Synteny	Chy3G065170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATTTTTAGGGAGCATGATTTTAATGGCGACGGTCGCTTGAGCATTAAAGAATTATCGAGAGCATTCGGGCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTGTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGGTAGATTATGCTATTAAATGTAAGTATAACCTACTTTAGTTATCATTTCTATCCTGCTGTGGAAGGTTCCCTAGTGAGTGAGTTGTTGATGTAAAAAAATAATATACAGAAAGGTACTTTGTGTGTGTTCAAGAGATACCTTCCCTATTATTGTAAAATGCCTTTGCTGATGGCTGATCCAAAGATGATAGCATTTCCAATAAAAATAAATTTTATGCTTCATTCTTGCTCTTGCATGATATGATAACAATTTTCTAAATATATATATTGTTTCAAAATAATAGACTTTATTTTAACAATGTACCAGGATCAGCTAACCTATTAATCTCTTATATCCAAAAGTTTCAAAAACAAATTACAAAATGATATAAACAACAATGATTAAGAAGTAATACAAAAATGCCAGTGTTGAAGTTTGCAACTTAATTAAAATTTTACAAATATATTGATGGATACACATCGTAGAGGAACGTCCACCAATATCAAAAGGTACGAAAATCAAACTAAACTGACCAATGTCCAATTATCTTCAAAATCGATTGGTTATTTGGTTCACTTTGGTCTAATTAATTTTGTTCGCCGTCATCTTGGCTTCGGTTTTATTAACAGTTTTTTGTATTCTCCATTTGTTATATTGGCCATCAAATCTAGGAAGAGAAGAGGGTGAAATAGAGTACGATGTTTAATTTGGGAGAGAAAGAGGCTCATGATTCTTTGAACCATAAACTTCGTCCTATTTGGATGTCAAGATTCATGTCTCATATAAGTATTTGGTAAATGGTAGAATGGTGAACCGAAGAAATATCCTCTCGTAAACGCTTGTTGAACATATAGCTCTCTCCATATGATGTACTGATTCAACATCTGATTTAGAAAACAAAACTAACATGGTGAAATTACAATTCTTTTTTTAAACTTTAGATACTGTTGTGTATTTTCATTTCTAAACTTTGAAAAAAAACCATTTACCTTCCTAAAATAAATAGTAAAATAATTCAAATAGTAAATAGTAAAACATACAAAAATTTCAAATTCTATCCAAGATAAACATTGATTAATTTCTATTGATATCTATGTTCATGCACTGTCTATTATTAAGAAAATCTTAAATATAAACTTTTGGTGGTAACAAACAACGTACTTCTTAACTATAATTATTACTCAAATTATTTTCTTAGCAAAATAACTAATGTTGTTAAACATTGTTCACAGTTAAGATGTAGAACCCTAATTTGCCCAAACAAACAACGAATACTCGAATTCTATTGTTAAAAATGTTTTCATTTAATTTATCCTCATATGTGTGTGGGTTTTTCTCTAGTGGGTTATTCATAATATAATATTTCGGTATACCTACTAATCCATCCTAGAATAATTTTTCAGAAAAGAAAAAAAAAACTATAAATTATAAAGAAATGAATAAAGAATAGACTAAAAATAGATTACTCAATCTTTCTTAAGTTATTATTCCAATCTTAAAGCTTAAGTTCAACAGATGATGTCAAAACACTATCTACGTCAATCACTATCTGATTTGCTACTTTTGCTAAGAATATAAAGTAGACTTTTCTATTTTTGACAACTTTTCTAAATTTTAATTTTTATAATTAAACAAAACGTACATAAAAAAGATATATTTACAAATAAAATTCGAAGTGAAATTCTATTTTAATGGAAAAGTAATTGACAAATTTTGTTATATTTTCAACTTTTTTTTAGAAAAAATATTTTTTTTAGAAAAAATATTTTTTTTAGAAAAGGATACTCATCATCCCCAATAATTATATTCAAAATAGGGCTCGGGGAAATAGTTCAAGTTTACAGGAAGGCTAGAGATAAGCCAAGACTATAAACCATACAAAAAACTAGCAAAAAACACTAAGGTGTTGTACAATTATAGAAGCTGAGTAATTTTTGAAAAGCTTGGTAGCCCATTGTGCTGTGAGATTGCAAATGTTTTCCCAAAGGTATGTATATGAGTGTGATAAGTTGTTGAATAGCCCGTTGTTTCTTTCCAACCAAATGGACCAAGTGATGGCAATCAACAGATTGAAGATAATCACCCCTCTTTGTGTCTTTATGTTGTTTGAACAGATCGTACTTATAAGTCCTGTGAGGTTATTATCAATGAGATCCCAATTTAATTGATTTTTGGCCATATTCCAAATGTTTCTGGTATATGGATAGTGGAGGAATATGTGGTCGGTTGTTTCATTGCTTTTCTTACAAAGTACGCACCAATTTGGGTTGAGACAAAGATTTTCCAATATTTTTCAAGATAAAATATTACTATATGTTTGGAATGTGCTACTTGTTGCATTTACCAATATTTTTAATCTTTGACCAATAAGTTTAAAATCCTAATTTAAGTAATGTTAGTAATTGCCTTGCACACACACACTTATCAGTTATCTTCATTCTCATAGGAATGACAATTGCATAAGCATAATAATTCCTTCTTTCACATTATTTCCAATTGTAAATAACAATATTGTTGAAAGTATCTACCTAATATAGTAAAATTTTCAATTCATCGATCAAGGGTTGATTTGAAAATTTCTATATTTTATGAATACATTGTTTCATTTTATCATATTTAAAAATGCTCAATTTATAATCTTAGTTCTAAAAAAATCCAAAACTTTTATTTCTATTTCTTCAACCCTTTCCACACAACCAAGCTCCAATATTTGAAAAAGAAAAGAAAACAAGGTATTCAAAATTAAAAGGTGTGGGAATTAAAACAGCCATAATTAACATATGAAAGGTTTTGTTTTTAATCAGTATGTTGTTTCTTTAAACACTTATTATTACGATTATGTAATAAATTTAAAACTTATTAGAGAAATGTTGTTGTTAGGTTTTTGGCGATTCAAACATATCGTAGTTTTTTAAAAAAGAAATAGAAAATAAAAAGGTAGATGAGAAAAAGAAAAAGGAACTAAGGAAGAGGATGGAAAACCTAAATCGGAGGTATGGTGATGGGGAACGGTAAGGCAAGTCGGAATCCATTTCCGGCAACGAAAAGTAATTAATAATGGAAAAAATACAAAGGTATGCTTGGATGCAGGAATGATTTATGGTGGGCTACTCACTCAAAATTATTCCTTTGTTCCTTCCTTTATTTATTTTGATGCACCACCACATTTTCAAATTACCACCATGAACCATGAATATTTTTTAAATATTAATATTATAAATATAGAAGAAAACGAAAACCTAAGTTAATATTTTGTGTAAATTTGGTAGTGAGGGTGAAAGTGGGAAGATAAGAATAGGGAACAAATGAATCTGCCACACCCATAATGAAGATGGTACCCAACTTGGAAATCTGAGCATCTTTCAACGAACAGTATTTTGGTATCTAGATAAGAAATGAATACTACGTCATTCACCATTTTCTAATGACATACTTTTAATGGTAAATACTTAGACGTTTCATGAAATTTATTTATGATTTATCTATGTAACGTTTACTAATTGGATGTTTAATTACAATTTTTTTTACCAATGACAAGATTTTTCATCTTTTTAGTTTTTCTTTTTCCTTTGATGATTGTAGATGCATTCAAGATCAATACAATCTCATTTTTAAGATTTGTGAAACTGTTAAAATAATTTTTGTCATATTAAAAACTAATTTAAATGTTTTTAGTAATTTTTCTATTGTATTGTTTCATAGCAAATATATATTAACTTTAAAAATTCGGTAGAAATGAGATAAAGATGTTAAAAAATGTTACATTTTTGCAATACGATCACGCACTCTTTCAACTAAACTACATATGCTCGATTTGACTAGTATGATTGCGTTGGAATCTGACACAAAATGGGTAGAAAATAAGTGGGTATTATAAGGCAAAAGCATCAAATATGCAACGACCATTATGTAACTCTTAAAGCCTAAAAGATAAAACGCAAATTGCCAAAAGCATCTTCCATCCTTTCCATTGTTGGAAAATTCATTAAGTACTTATTAATTACGATAACAAAGGAACAATTGGATTGTGAATTTGTCACTCCTTAATCACTTTGTGGATTTGGGTTGTCAACTTTAACCCTTTAACTTTCAAATTTTAGGAATTGAATTCTAGAATCTAGTGAGTATTAAAATTAATACACGTTTAAATTGGTATTAAATTTGTTTTTAATGGTAGATGTGTTTTGGTCTCCTTTAAGATATATATCTCTTACTATGCACATTCATTTCCTCTGCCTCCTTGTTAAAAGAAAATTGGTATAAAACAAAGATTTCCAAAAACCAACAAATAAATCATAATCTTAAAAGGGAAAAAAAAAGAGTAAAAGCCAGAGTCTTGTCAAATTAATTATAAGAATCCTCATAACTTTAACTGCAAGTTCTAGAAACAAAAGGAATAAATTTAATTGATATCATTCTTTTATTAAATGTTTCAAAGTAATTAAAACTATACATTGTTAGTATTAATTATTTGACAAGGTGTAACATATAATACCCTATTAACAATGAGAATGTACCTAAATTTCATGCATTCCATTTTTGACAAATTTTTATTGAATATATTATTTTTATTACAAAAATGGGTGCAACCCAAACTTTATCTAAAAGTTTTTCATGCAACAATATATCATGGCTAACTTTACAAGAGAAATAAGGCTTTACATTCAAAGAAAAGAAAATGTAATAGATAAGGATAATTATTAAATTCTTTTTGATGCATGAAGACTTTTTGAATTGATAATGAAATCAGAAAAATATTGACTACTTTCAAGACACGTATTCGATTCTTCACGTGAGCTTCATAAAGTTGATGTTAAACGTTTTGGCAGTATCCTCTTTTTTCTTTTTTTCTTTTTTTTTCCATTTTATTATTTTTATATAAAGAAAAATGAAATTCTCCTTTCTAAAATAATCAGAAAATACCTCATAAAAAAAAGAGAAAAAAATAGTAAAATGTAATATTTTAATTCTAAGAAAAGATTCCATATATCATTATGATACCAATATGATTACAAAGAAAGTGGGAGAATTCTACTATAAATACCCCACGATCCATACATCGATAACCACCAAGCAAACCATAAAAAAGATTACGAACATTTTGCTCCATCACTAAACCGTATCATTTCTAAGAAAATGTCTCGTGCAGCAACCAGGCTACAGCTCTCCAGGGAGGATATAAAGGAGATTTTTAGAGAGCATGACATCGATGGAGATGGTCGATTGAGCATGAACGAGATGGTGAAGGCCTTCGGGTTCTTTGGCAGTATTCTTCCTTTCTACAAAGCACACTACGGAATGGCTCATGCTGATGAGAATGGCGATGGCTTCATCGAAGAGGAGGAGCTCGACAAGCTCGTCGACTATGCCCAGAAGTTTCAGCACAGGAGGAAGTGCTGA

mRNA sequence

ATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATTTTTAGGGAGCATGATTTTAATGGCGACGGTCGCTTGAGCATTAAAGAATTATCGAGAGCATTCGGGCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTGTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGAAAATGTCTCGTGCAGCAACCAGGCTACAGCTCTCCAGGGAGGATATAAAGGAGATTTTTAGAGAGCATGACATCGATGGAGATGGTCGATTGAGCATGAACGAGATGGTGAAGGCCTTCGGGTTCTTTGGCAGTATTCTTCCTTTCTACAAAGCACACTACGGAATGGCTCATGCTGATGAGAATGGCGATGGCTTCATCGAAGAGGAGGAGCTCGACAAGCTCGTCGACTATGCCCAGAAGTTTCAGCACAGGAGGAAGTGCTGA

Coding sequence (CDS)

ATGACTCGTCAATTATATGACAAGAAGAAATCAGGGTACAAGTTGCAAAAAGAAGAAATGAAAGAAATTTTTAGGGAGCATGATTTTAATGGCGACGGTCGCTTGAGCATTAAAGAATTATCGAGAGCATTCGGGCAATTTGGCGCATTCTTCCCTCTCTACCGTGCAGCTTGTGGGTTGTTCCTTGCAGATGATGACGGCGATGGATTTATCAGTGAGCAAGAACTAGATAAAGTGAAAATGTCTCGTGCAGCAACCAGGCTACAGCTCTCCAGGGAGGATATAAAGGAGATTTTTAGAGAGCATGACATCGATGGAGATGGTCGATTGAGCATGAACGAGATGGTGAAGGCCTTCGGGTTCTTTGGCAGTATTCTTCCTTTCTACAAAGCACACTACGGAATGGCTCATGCTGATGAGAATGGCGATGGCTTCATCGAAGAGGAGGAGCTCGACAAGCTCGTCGACTATGCCCAGAAGTTTCAGCACAGGAGGAAGTGCTGA

Protein sequence

MTRQLYDKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQELDKVKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHADENGDGFIEEEELDKLVDYAQKFQHRRKC*

Homology

[Close]

BLAST of Chy3G065170 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.5e-12
Identity = 46/134 (34.33%), Postives = 65/134 (48.51%), Query Frame = 0


Query: 17  KEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQEL 76
           +EEM+ +FR+ D NGDGR+S  EL   F   G        A  +  AD DGDGFIS  E 
Sbjct: 38  EEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEF 97

Query: 77  DKVKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMA 136
             +  + +     +  ED++  FR  D DG+G +S  E+ +     G      +    + 
Sbjct: 98  AALNATASGDAAAV-EEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIE 157

Query: 137 HADENGDGFIEEEE 151
             D+NGDG I  EE
Sbjct: 158 GVDQNGDGLISFEE 170

[Close]

BLAST of Chy3G065170 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 8.8e-10
Identity = 39/137 (28.47%), Postives = 67/137 (48.91%), Query Frame = 0


Query: 19  EMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQELDK 78
           E++E+F++ D NGDG++S  EL+      G+          +  AD DGDG++S QE   
Sbjct: 26  ELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAEVKAMMEEADTDGDGYVSLQEFVD 85

Query: 79  VKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHA 138
           + +  A  +      D+K  F+  D D +G +S  E+ +     G      ++   + + 
Sbjct: 86  LNIKGATVK------DLKNAFKVFDRDCNGTISPAELCETLKSVGEPCTIEESKNIIHNV 145

Query: 139 DENGDGFIEEEELDKLV 156
           D+NGDG I  EE   ++
Sbjct: 146 DKNGDGLINVEEFQTMM 156

[Close]

BLAST of Chy3G065170 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-09
Identity = 41/145 (28.28%), Postives = 70/145 (48.28%), Query Frame = 0


Query: 6   YDKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADD 65
           Y +K    K +  E++ +F++ D NGDG++S KEL       G   P       +   D 
Sbjct: 24  YLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDR 83

Query: 66  DGDGFISEQELDKVKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSI 125
            GDG+I+ +E   V+++          E++K+ F  +DIDG+G +S  E+ +     G  
Sbjct: 84  KGDGYINFEEF--VELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE 143

Query: 126 LPFYKAHYGMAHADENGDGFIEEEE 151
               +    +   D++GDG I+ EE
Sbjct: 144 CSIAECRKMIGGVDKDGDGTIDFEE 166

[Close]

BLAST of Chy3G065170 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-09
Identity = 40/137 (29.20%), Postives = 65/137 (47.45%), Query Frame = 0


Query: 19  EMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQELDK 78
           E+K++F + D NGDG++S+ EL   F   G  +        L   D D DGFI+++E   
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 79  VKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHA 138
           +       R   S  +I+E F  +D + +G +S +E+ K     G           + H 
Sbjct: 80  I------CRSSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHV 139

Query: 139 DENGDGFIEEEELDKLV 156
           D +GDG +  EE  K++
Sbjct: 140 DTDGDGNVNFEEFQKMM 150

[Close]

BLAST of Chy3G065170 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 3.3e-09
Identity = 39/137 (28.47%), Postives = 66/137 (48.18%), Query Frame = 0


Query: 19  EMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQELDK 78
           E++E+F++ D NGDG++S  EL+      G+          +  AD DGDG++S QE   
Sbjct: 26  ELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEFVD 85

Query: 79  VKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHA 138
           +    A+ +      D+K  F+  D D +G +S  E+       G      ++   + + 
Sbjct: 86  LNNKGASVK------DLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 145

Query: 139 DENGDGFIEEEELDKLV 156
           D+NGDG I  EE   ++
Sbjct: 146 DKNGDGLISVEEFQTMM 156

[Close]

BLAST of Chy3G065170 vs. ExPASy TrEMBL
Match: A0A0A0LRC1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 4.5e-41
Identity = 85/87 (97.70%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 81  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHADE 140
           MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFG FGSILPFYKAHYGMAHADE
Sbjct: 1   MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 141 NGDGFIEEEELDKLVDYAQKFQHRRKC 168
           NGDGFIEEEELDKLVDYAQKFQH+RKC
Sbjct: 61  NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of Chy3G065170 vs. ExPASy TrEMBL
Match: A0A6J1CSH1 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111013915 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 1.1e-39
Identity = 87/160 (54.37%), Postives = 109/160 (68.12%), Query Frame = 0


Query: 7   DKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDD 66
           +KK    K  + ++KEIF EHD +GDG LS+ EL +AF   G+  PLY+A  GL  ADDD
Sbjct: 65  EKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDD 124

Query: 67  GDGFISEQELDKV------KMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFG 126
           GDG ISE+ELDK+         R  + + LSRE +KEIF+ HD + DG L++ E+ KAFG
Sbjct: 125 GDGLISEEELDKLVDYAEKGSDRKTSNMPLSREHMKEIFKYHDSNKDGFLNVRELTKAFG 184

Query: 127 FFGSILPFYKAHYGMAHADENGDGFIEEEELDKLVDYAQK 161
           F GSI+PF KAHYG+ HAD N DG I E ELDKL+DYA K
Sbjct: 185 FLGSIIPFCKAHYGLVHADANNDGLINETELDKLIDYAYK 224

[Close]

BLAST of Chy3G065170 vs. ExPASy TrEMBL
Match: A0A5A7UYC0 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00250 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.4e-39
Identity = 80/87 (91.95%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 81  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHADE 140
           MSRAA++LQLSREDIKEIFREHDIDGDGRLS++EMVKAFGFFGSILPFYKAHYGMAHADE
Sbjct: 1   MSRAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADE 60

Query: 141 NGDGFIEEEELDKLVDYAQKFQHRRKC 168
           NGDG+IEEEELDKLVDYA+KFQH+RKC
Sbjct: 61  NGDGYIEEEELDKLVDYAEKFQHKRKC 87

[Close]

BLAST of Chy3G065170 vs. ExPASy TrEMBL
Match: A0A0A0LKW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.4e-34
Identity = 77/79 (97.47%), Postives = 78/79 (98.73%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGL 60
          MTRQLYDKKKSGYKLQKEEMKE+FREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAA GL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKV 80
          FLADDDGDGFISEQELDKV
Sbjct: 61 FLADDDGDGFISEQELDKV 79

[Close]

BLAST of Chy3G065170 vs. ExPASy TrEMBL
Match: A0A5A7UY46 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00260 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.7e-32
Identity = 73/79 (92.41%), Postives = 76/79 (96.20%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGL 60
          MTR LYDKKKSGYKLQKEEMK+IFREHDFNGDGRLSIKELSRAFG FGAFFPLYRAA GL
Sbjct: 1  MTRPLYDKKKSGYKLQKEEMKQIFREHDFNGDGRLSIKELSRAFGSFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKV 80
          F+ADDDGDGFISEQEL+KV
Sbjct: 61 FVADDDGDGFISEQELEKV 79

[Close]

BLAST of Chy3G065170 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 311 bits (798), Expect = 1.34e-106
Identity = 157/168 (93.45%), Postives = 161/168 (95.83%), Query Frame = 0


Query: 1   MTRQLYDKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGL 60
           MTRQLYDKKKSGYKLQKEEMKE+FREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAA GL
Sbjct: 1   MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61  FLADDDGDGFISEQELDKV-KMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAF 120
           FLADDDGDGFISEQELDKV   +  +TRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAF
Sbjct: 61  FLADDDGDGFISEQELDKVVDYAIKSTRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAF 120

Query: 121 GFFGSILPFYKAHYGMAHADENGDGFIEEEELDKLVDYAQKFQHRRKC 167
           G FGSILPFYKAHYGMAHADENGDGFIEEEELDKLVDYAQKFQH+RKC
Sbjct: 121 GCFGSILPFYKAHYGMAHADENGDGFIEEEELDKLVDYAQKFQHKRKC 168

[Close]

BLAST of Chy3G065170 vs. NCBI nr
Match: KGN62516.1 (hypothetical protein Csa_022521 [Cucumis sativus])

HSP 1 Score: 177 bits (448), Expect = 2.00e-54
Identity = 85/87 (97.70%), Postives = 86/87 (98.85%), Query Frame = 0


Query: 81  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHADE 140
           MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFG FGSILPFYKAHYGMAHADE
Sbjct: 1   MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 141 NGDGFIEEEELDKLVDYAQKFQHRRKC 167
           NGDGFIEEEELDKLVDYAQKFQH+RKC
Sbjct: 61  NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of Chy3G065170 vs. NCBI nr
Match: KAA0060892.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 172 bits (435), Expect = 1.90e-52
Identity = 80/87 (91.95%), Postives = 87/87 (100.00%), Query Frame = 0


Query: 81  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHADE 140
           MSRAA++LQLSREDIKEIFREHDIDGDGRLS++EMVKAFGFFGSILPFYKAHYGMAHADE
Sbjct: 1   MSRAASKLQLSREDIKEIFREHDIDGDGRLSLSEMVKAFGFFGSILPFYKAHYGMAHADE 60

Query: 141 NGDGFIEEEELDKLVDYAQKFQHRRKC 167
           NGDG+IEEEELDKLVDYA+KFQH+RKC
Sbjct: 61  NGDGYIEEEELDKLVDYAEKFQHKRKC 87

[Close]

BLAST of Chy3G065170 vs. NCBI nr
Match: XP_022144156.1 (probable calcium-binding protein CML15 [Momordica charantia])

HSP 1 Score: 170 bits (430), Expect = 8.97e-50
Identity = 87/160 (54.37%), Postives = 109/160 (68.12%), Query Frame = 0


Query: 7   DKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDD 66
           +KK    K  + ++KEIF EHD +GDG LS+ EL +AF   G+  PLY+A  GL  ADDD
Sbjct: 65  EKKAPKIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDD 124

Query: 67  GDGFISEQELDKVK------MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFG 126
           GDG ISE+ELDK+         R  + + LSRE +KEIF+ HD + DG L++ E+ KAFG
Sbjct: 125 GDGLISEEELDKLVDYAEKGSDRKTSNMPLSREHMKEIFKYHDSNKDGFLNVRELTKAFG 184

Query: 127 FFGSILPFYKAHYGMAHADENGDGFIEEEELDKLVDYAQK 160
           F GSI+PF KAHYG+ HAD N DG I E ELDKL+DYA K
Sbjct: 185 FLGSIIPFCKAHYGLVHADANNDGLINETELDKLIDYAYK 224

[Close]

BLAST of Chy3G065170 vs. NCBI nr
Match: KGN62515.1 (hypothetical protein Csa_022644 [Cucumis sativus])

HSP 1 Score: 154 bits (388), Expect = 3.03e-45
Identity = 77/79 (97.47%), Postives = 78/79 (98.73%), Query Frame = 0


Query: 1  MTRQLYDKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGL 60
          MTRQLYDKKKSGYKLQKEEMKE+FREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAA GL
Sbjct: 1  MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 61 FLADDDGDGFISEQELDKV 79
          FLADDDGDGFISEQELDKV
Sbjct: 61 FLADDDGDGFISEQELDKV 79

[Close]

BLAST of Chy3G065170 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 40/137 (29.20%), Postives = 65/137 (47.45%), Query Frame = 0


Query: 19  EMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQELDK 78
           E+K++F + D NGDG++S+ EL   F   G  +        L   D D DGFI+++E   
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 79  VKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAHA 138
           +       R   S  +I+E F  +D + +G +S +E+ K     G           + H 
Sbjct: 80  I------CRSSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHV 139

Query: 139 DENGDGFIEEEELDKLV 156
           D +GDG +  EE  K++
Sbjct: 140 DTDGDGNVNFEEFQKMM 150

[Close]

BLAST of Chy3G065170 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-10
Identity = 41/145 (28.28%), Postives = 70/145 (48.28%), Query Frame = 0


Query: 6   YDKKKSGYKLQKEEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADD 65
           Y +K    K +  E++ +F++ D NGDG++S KEL       G   P       +   D 
Sbjct: 24  YLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDR 83

Query: 66  DGDGFISEQELDKVKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSI 125
            GDG+I+ +E   V+++          E++K+ F  +DIDG+G +S  E+ +     G  
Sbjct: 84  KGDGYINFEEF--VELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE 143

Query: 126 LPFYKAHYGMAHADENGDGFIEEEE 151
               +    +   D++GDG I+ EE
Sbjct: 144 CSIAECRKMIGGVDKDGDGTIDFEE 166

[Close]

BLAST of Chy3G065170 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-08
Identity = 38/138 (27.54%), Postives = 63/138 (45.65%), Query Frame = 0


Query: 18  EEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQELD 77
           EE+K++F + D NGDG++S+ EL   F   G  +        L   D D DG+I+  E  
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 78  KVKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAH 137
            +       R   S  +I++ F  +D D +G +S +E+ +     G           +  
Sbjct: 82  TL------CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGP 141

Query: 138 ADENGDGFIEEEELDKLV 156
            D +GDG +  EE  K++
Sbjct: 142 VDADGDGNVNFEEFQKMM 153

[Close]

BLAST of Chy3G065170 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-08
Identity = 38/138 (27.54%), Postives = 63/138 (45.65%), Query Frame = 0


Query: 18  EEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQELD 77
           EE+K++F + D NGDG++S+ EL   F   G  +        L   D D DG+I+  E  
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 78  KVKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYGMAH 137
            +       R   S  +I++ F  +D D +G +S +E+ +     G           +  
Sbjct: 82  TL------CRSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGP 141

Query: 138 ADENGDGFIEEEELDKLV 156
            D +GDG +  EE  K++
Sbjct: 142 VDADGDGNVNFEEFQKMM 153

[Close]

BLAST of Chy3G065170 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 55.1 bits (131), Expect = 6.4e-08
Identity = 36/141 (25.53%), Postives = 64/141 (45.39%), Query Frame = 0


Query: 18  EEMKEIFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAACGLFLADDDGDGFISEQE-- 77
           +++K++F+  D NGDG++S+ EL                   +   D DG+GFI   E  
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75

Query: 78  -LDKVKMSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGFFGSILPFYKAHYG 137
            L ++ +       +    D+KE F  +D+DG+GR+S  E+       G           
Sbjct: 76  ALFQIGIGGGGNN-RNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKM 135

Query: 138 MAHADENGDGFIEEEELDKLV 156
           ++  D +GDG +  +E  K++
Sbjct: 136 ISKVDIDGDGCVNFDEFKKMM 155

The following BLAST results are available for this feature:

BLAST of Chy3G065170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	2.5e-12	34.33	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
A0T2M3	8.8e-10	28.47	Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1	[more]
Q9FYK2	2.5e-09	28.28	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q9C9U8	2.5e-09	29.20	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]
O64943	3.3e-09	28.47	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]

BLAST of Chy3G065170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC1	4.5e-41	97.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358870 PE=4 SV=1	[more]
A0A6J1CSH1	1.1e-39	54.38	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5A7UYC0	1.4e-39	91.95	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LKW4	1.4e-34	97.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358860 PE=4 SV=1	[more]
A0A5A7UY46	1.7e-32	92.41	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Chy3G065170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152805.1	1.34e-106	93.45	probable calcium-binding protein CML10 [Cucumis sativus]	[more]
KGN62516.1	2.00e-54	97.70	hypothetical protein Csa_022521 [Cucumis sativus]	[more]
KAA0060892.1	1.90e-52	91.95	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
XP_022144156.1	8.97e-50	54.38	probable calcium-binding protein CML15 [Momordica charantia]	[more]
KGN62515.1	3.03e-45	97.47	hypothetical protein Csa_022644 [Cucumis sativus]	[more]

BLAST of Chy3G065170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73630.1	1.8e-10	29.20	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.8e-10	28.28	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.3e-08	27.54	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	1.3e-08	27.54	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	6.4e-08	25.53	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 130..158 e-value: 45.0 score: 4.4 coord: 94..122 e-value: 0.023 score: 23.8 coord: 19..47 e-value: 5.4E-4 score: 29.3 coord: 53..83 e-value: 93.0 score: 1.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 62..119 e-value: 3.2E-10 score: 40.3
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 19..45 e-value: 1.7E-6 score: 27.4
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 138..155 e-value: 0.0042 score: 16.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 15..50 score: 14.457615
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 90..125 score: 14.373928
None	No IPR available	GENE3D	1.10.238.10		coord: 87..164 e-value: 1.3E-11 score: 45.9 coord: 6..86 e-value: 5.4E-11 score: 43.9
None	No IPR available	PANTHER	PTHR10891:SF911	SUBFAMILY NOT NAMED	coord: 9..79
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 86..161
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 9..79
None	No IPR available	PANTHER	PTHR10891:SF911	SUBFAMILY NOT NAMED	coord: 86..161
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 139..151
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 103..115
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 28..40
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 16..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G065170.1	Chy3G065170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding