Chy3G064830 (gene) Cucumber (hystrix) v1

Overview
NameChy3G064830
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.5-like
LocationchrH03: 17388459 .. 17390980 (-)
RNA-Seq ExpressionChy3G064830
SyntenyChy3G064830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTTGTGTCGGTGGCGTTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCAGTGACCACTCTCACTAACTTTATGGGAACAACGTTTCTTCTCACACTACTCGGGGGCTTCATTTCCGAAACTTATCTCTCAAGATTCAAGACTTGCCTTCTCTTTGCTTTCTTTGAGCTCACGGTCAACTCTCTTTCTCTTTCTCTTCCCTTCATTTCCATTTTTGTTTACATTAGACCCAATCTCCGTTTGATTTGACAGCCCATGTTGTTCTATTACATATTCCATCCTCAAACTAAAATAGCCTATATATACTATACTATCGTCGTGGGTAAACGGATGCTATTTTTCTTTATTTACGTTTGATTTTAGTCTTTTAGAAAATTATAAACTGTAGTTGAATAATATTTAATTTGAGAAAAATACTTTGTTGCATGCTAGTGGGAGGGACCCTCTCTGCATTTCATATAAATAAATAAAACAATAAAAATAAAATATAGCCACGACCGTATCAAAATATTGGCCAATGTGAGCGTGTGAGATTTTGGTTGAATAAGAATCATTTCTTTATCCATGCACCGTGATATGGATTTATATTATTAGAATCATCCTATTAGGAAAAACATATTTCATAGTGATTTTGAAAACAAGGATGAGTGCATATAGAGTACATATTTTTATATCAATTGGGCTATGTTTAGATGGAGTGATTTTGTTTAGAAATAGAAAGCCAAAAGTGTTTTAGATACCAAGCTATGATGATGATAAAAGTAAACATTTGTGCTCAAGATATTAGCATCATGATCTTCGACCTCATCCCCACCCACACGCTGTTTTGTTGAAAAATAATAACAATGACTAAAATAGTTATTATGTGGACTATTGTGCAGGGATATTGTCTGCTAACGGTGCAGGCACATTTCCCGCACCTAAGGCCGGCACCTTGCAAAGAGGCACCAGCAGGGAGCGAGTGCGAGGCGGCAGGGAGCAGTCAGGAGGCGTTTCTTTACACGGGATTATACCTGATAGCCCTGGGAACGAGTGGCGTGAAAGCAGCGCTGCCAGCATTGGGGGCAGATCAATTCGATGACAGAGATCCAAGCGAGGCGGGGAAAGTTTCGAGCTTTTTCAACTGGTTCCTGTTCAGCCTAACAATAGGGTCAATTGTGGGATTGACATTGATAGTGTGGATAAACACAGAGGTAGGTTGGGACTGGGCGTTTGTGGTTTGCAGCATTGCAGTTCTTGCCGCCATTGTTGTGGTGTTTTTAGGCAAGTCCTTTTATCGCCACAATGTGCCTCAAGGAAGCCCCATTCTTAGACTTCTACAGGTTCATATTTCTTTGCCTGATATGCAGTCTCTTTGAACTCATCAATCATGGTGCAATGAATGGTCGTTTGATCCTTTTTATTCTCATGTATAGGTCTTTGTTGCTTCAATCCGAAACAGGAAGCTTCCACTTCCAGAGAATGCTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAGTACTCGAGAAAACTGATCAATTCAGGTACTATACTTTTTCATGATCTTCATATAAAGAACTAAGCTTTGCAATACAATTTGTTGGTTGAAGCCATGAAATTTTGGAACTTTGTAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAATTGCATCAATATCAACAAATCAGCAAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAGGAAACAAAGATCCTAATTAGAATGCTCCCAATTATACTCAGCACCATCTTCATGGCCACCTGCATGGCTCAACTCCAAACATTCTCCATTCAACAAAGCATAACCATGGATTCCCAGTTCCTGGGATTCAAAATTCCGGGCCCCTCCATCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTGTTTTCGTTCCTCTCGCTCGTAAAATTACTGGGATTCCAACAGGAATTCGTCACCTCCAGAGAATTGGCATTGGACTCGTACTCACTGCCGCATCGATGGCCATTGCTGGGTTTGTAGAGACTCGACGCAAAAACGTTGCAATCAAACACAATATGGTGGATAGTACAGAGCCACTGCCGATCAGCGTTTTCTGGTTGGGATTTCAGTTCTGCGTATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAGAGCTCAGCAGGGATGAAGTCGTTGAGCACAGCTATTGCTTGGTGTGCGATTGCATTCGGGTATTTCACGAGTACGGTGGTGGTTACAGTGGTGAACAAGGCGAGTGGAGGGTGGCTGGCAAGCAACAATCTCAATAGAGACAAGTTGGACTATTTCTACTGGTTATTGTCAGTGTTGAGTGTGTTGAATTTTGGGTTTTATTTGGTTTGTGCTTCCTGGTATAGGTATAAGAATGTGGAGATCCATCAAAACGATGCTATGGAAGAAAAGGTTGACATAGCAAGGGAGGCATAG

mRNA sequence

ATGGCATTTGTGTCGGTGGCGTTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCAGTGACCACTCTCACTAACTTTATGGGAACAACGTTTCTTCTCACACTACTCGGGGGCTTCATTTCCGAAACTTATCTCTCAAGATTCAAGACTTGCCTTCTCTTTGCTTTCTTTGAGCTCACGGGATATTGTCTGCTAACGGTGCAGGCACATTTCCCGCACCTAAGGCCGGCACCTTGCAAAGAGGCACCAGCAGGGAGCGAGTGCGAGGCGGCAGGGAGCAGTCAGGAGGCGTTTCTTTACACGGGATTATACCTGATAGCCCTGGGAACGAGTGGCGTGAAAGCAGCGCTGCCAGCATTGGGGGCAGATCAATTCGATGACAGAGATCCAAGCGAGGCGGGGAAAGTTTCGAGCTTTTTCAACTGGTTCCTGTTCAGCCTAACAATAGGGTCAATTGTGGGATTGACATTGATAGTGTGGATAAACACAGAGGTAGGTTGGGACTGGGCGTTTGTGGTTTGCAGCATTGCAGTTCTTGCCGCCATTGTTGTGGTGTTTTTAGGCAAGTCCTTTTATCGCCACAATGTGCCTCAAGGAAGCCCCATTCTTAGACTTCTACAGGTCTTTGTTGCTTCAATCCGAAACAGGAAGCTTCCACTTCCAGAGAATGCTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAGTACTCGAGAAAACTGATCAATTCAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAATTGCATCAATATCAACAAATCAGCAAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAGGAAACAAAGATCCTAATTAGAATGCTCCCAATTATACTCAGCACCATCTTCATGGCCACCTGCATGGCTCAACTCCAAACATTCTCCATTCAACAAAGCATAACCATGGATTCCCAGTTCCTGGGATTCAAAATTCCGGGCCCCTCCATCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTGTTTTCGTTCCTCTCGCTCGTAAAATTACTGGGATTCCAACAGGAATTCGTCACCTCCAGAGAATTGGCATTGGACTCGTACTCACTGCCGCATCGATGGCCATTGCTGGGTTTGTAGAGACTCGACGCAAAAACGTTGCAATCAAACACAATATGGTGGATAGTACAGAGCCACTGCCGATCAGCGTTTTCTGGTTGGGATTTCAGTTCTGCGTATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAGAGCTCAGCAGGGATGAAGTCGTTGAGCACAGCTATTGCTTGGTGTGCGATTGCATTCGGGTATTTCACGAGTACGGTGGTGGTTACAGTGGTGAACAAGGCGAGTGGAGGGTGGCTGGCAAGCAACAATCTCAATAGAGACAAGTTGGACTATTTCTACTGGTTATTGTCAGTGTTGAGTGTGTTGAATTTTGGGTTTTATTTGGTTTGTGCTTCCTGGTATAGGTATAAGAATGTGGAGATCCATCAAAACGATGCTATGGAAGAAAAGGTTGACATAGCAAGGGAGGCATAG

Coding sequence (CDS)

ATGGCATTTGTGTCGGTGGCGTTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCAGTGACCACTCTCACTAACTTTATGGGAACAACGTTTCTTCTCACACTACTCGGGGGCTTCATTTCCGAAACTTATCTCTCAAGATTCAAGACTTGCCTTCTCTTTGCTTTCTTTGAGCTCACGGGATATTGTCTGCTAACGGTGCAGGCACATTTCCCGCACCTAAGGCCGGCACCTTGCAAAGAGGCACCAGCAGGGAGCGAGTGCGAGGCGGCAGGGAGCAGTCAGGAGGCGTTTCTTTACACGGGATTATACCTGATAGCCCTGGGAACGAGTGGCGTGAAAGCAGCGCTGCCAGCATTGGGGGCAGATCAATTCGATGACAGAGATCCAAGCGAGGCGGGGAAAGTTTCGAGCTTTTTCAACTGGTTCCTGTTCAGCCTAACAATAGGGTCAATTGTGGGATTGACATTGATAGTGTGGATAAACACAGAGGTAGGTTGGGACTGGGCGTTTGTGGTTTGCAGCATTGCAGTTCTTGCCGCCATTGTTGTGGTGTTTTTAGGCAAGTCCTTTTATCGCCACAATGTGCCTCAAGGAAGCCCCATTCTTAGACTTCTACAGGTCTTTGTTGCTTCAATCCGAAACAGGAAGCTTCCACTTCCAGAGAATGCTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAGTACTCGAGAAAACTGATCAATTCAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAATTGCATCAATATCAACAAATCAGCAAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAGGAAACAAAGATCCTAATTAGAATGCTCCCAATTATACTCAGCACCATCTTCATGGCCACCTGCATGGCTCAACTCCAAACATTCTCCATTCAACAAAGCATAACCATGGATTCCCAGTTCCTGGGATTCAAAATTCCGGGCCCCTCCATCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTGTTTTCGTTCCTCTCGCTCGTAAAATTACTGGGATTCCAACAGGAATTCGTCACCTCCAGAGAATTGGCATTGGACTCGTACTCACTGCCGCATCGATGGCCATTGCTGGGTTTGTAGAGACTCGACGCAAAAACGTTGCAATCAAACACAATATGGTGGATAGTACAGAGCCACTGCCGATCAGCGTTTTCTGGTTGGGATTTCAGTTCTGCGTATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAGAGCTCAGCAGGGATGAAGTCGTTGAGCACAGCTATTGCTTGGTGTGCGATTGCATTCGGGTATTTCACGAGTACGGTGGTGGTTACAGTGGTGAACAAGGCGAGTGGAGGGTGGCTGGCAAGCAACAATCTCAATAGAGACAAGTTGGACTATTTCTACTGGTTATTGTCAGTGTTGAGTGTGTTGAATTTTGGGTTTTATTTGGTTTGTGCTTCCTGGTATAGGTATAAGAATGTGGAGATCCATCAAAACGATGCTATGGAAGAAAAGGTTGACATAGCAAGGGAGGCATAG

Protein sequence

MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLFAFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKEAAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIAREA*
Homology
BLAST of Chy3G064830 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 5.7e-111
Identity = 219/529 (41.40%), Postives = 329/529 (62.19%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL LLGGF+++ + S F   L+ 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  G  LLT+QA  P L P PCK + A   CE  G S+ AFL+ GLYL++LG  G+K
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSS-AALRCEVVGGSKAAFLFVGLYLVSLGIGGIK 165

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V +T +VWI    GW+W F V 
Sbjct: 166 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 225

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFV-ASIRNRKLPLPENANELHEIR-D 240
           +I++  +I+V  LG  FY++ +P+GSP+  + +V + ASI +       N     E++ +
Sbjct: 226 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 285

Query: 241 KEAAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          I       W  CTV QVE+ KI+++MLP
Sbjct: 286 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 345

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
           I   TI +  C+AQL T+S+ Q+ TM+ + + F +P  S+PV P++FM    P Y+ + +
Sbjct: 346 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 405

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A  VE +RK VA +  ++DS E LPI+ 
Sbjct: 406 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 465

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W ++A GY+ S+V+V +VN
Sbjct: 466 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 525

Query: 481 KAS-----GGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRY 523
           + +       WL    LNR++LD FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 526 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Chy3G064830 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.1e-109
Identity = 217/541 (40.11%), Postives = 332/541 (61.37%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +A+++ A +LV Y   YM+ S +KS   +TNFMGT FLL LLGGF+S+ + S F+  L+ 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  G  +LT+QA  P L P  C ++P    CE    S+ A L+ GLYL+ALG  G+K
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSC-DSPT---CEEVSGSKAAMLFVGLYLVALGVGGIK 163

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +L + GA+QFD+  P    + S+FFN+F+F L  G++V +T +VW+    GW+W F V 
Sbjct: 164 GSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVS 223

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVA---------SIRNRKLPLPENAN 240
           +IA+  +I++   G  FYR+ +P GSP+  +L+V +A         S  N    +  + +
Sbjct: 224 TIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS 283

Query: 241 ELHEIRDKEAAIPYEVLEKTDQFRFL-DRAAIIRNDTIASISTNQQGPWRL--CTVTQVE 300
                + K+       LEK  Q   L  RA +  +  + + + +++   RL  CTV QVE
Sbjct: 284 NHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVE 343

Query: 301 ETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFF 360
           + KI+++MLPI   TI +  C+AQL TFS+QQ+ +M+++    KIP  S+P+ P++F+  
Sbjct: 344 DVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMI 403

Query: 361 FIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMV 420
             P Y+ + +P ARK T   TG+ HLQRIG+GLVL+  +MA+A  VE +RK VA    ++
Sbjct: 404 LAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLL 463

Query: 421 DSTEPLPISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYF 480
           DS E LP++  W+  Q+   G  D+FTL GLLE+F+ E+ + M+SL+T+++W ++A GY+
Sbjct: 464 DSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYY 523

Query: 481 TSTVVVTVVNKASGG-----WLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYK 525
            S+V+V++VN  +G      WL   ++NR KLDYFYWL+ VLS  NF  YL  A  Y+Y+
Sbjct: 524 LSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 580

BLAST of Chy3G064830 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.8e-109
Identity = 226/549 (41.17%), Postives = 334/549 (60.84%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MA  +V  +L+TY    M+F L+KS   +TNF+GT FLL+LLGGF+S++YL  F+T L+F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
              E++G+ LL+VQAH P LRP  C        C  A   + A LYT L L+ALG+  +K
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + + GA+QF  +D     K+SSFFN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           +  + A ++ +  G SFYR+  P GS    + QVFVA+I  RK   P N N +H+     
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQ--PST 296

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
             +  + L  +++FRFLD+A       I +     + PWRLCT+ QV + KIL+ ++PI 
Sbjct: 297 DLVRVKPLLHSNKFRFLDKAC------IKTQGKAMESPWRLCTIEQVHQVKILLSVIPIF 356

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFVP 360
             TI   T +AQLQTFS+QQ  +M++     F+IP  S+  IP + + FF+P YE  FVP
Sbjct: 357 ACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVP 416

Query: 361 LARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVF 420
           LARK+TG  +GI  LQRIG GL L   SM  A  VE +R+   ++ N++       +S+F
Sbjct: 417 LARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM-------LSIF 476

Query: 421 WLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNK 480
           W+  QF +FG+ ++FT VGL+EFFY +SS  M+S  TA+ +C+ +FG++ S+V+V+ VN+
Sbjct: 477 WIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNR 536

Query: 481 ASG--------GWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWY------RYKNV 535
            +         GWL  N+LN+D+LD+FYWLL+ LS +NF  YL  + WY       + + 
Sbjct: 537 VTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSATHHSA 587

BLAST of Chy3G064830 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 8.0e-105
Identity = 216/527 (40.99%), Postives = 319/527 (60.53%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           M   +V  +L+TY    M+F L+K+   +TNF+GT F+  LLGG++S+ +L  F T ++F
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
            F EL+G+ LL+VQAH P L+P  C        CE A   +    +  LYL+ALG+  VK
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKCNPL-IDQTCEEAKGFKAMIFFMALYLVALGSGCVK 179

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + A GADQF    P ++ ++SS+FN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 180 PNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVS 239

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELH-EIRDK 240
           + A+   I+ +  G  ++R+  P+ S    +  V VA+I  RKL  P +   LH +    
Sbjct: 240 AAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVA 299

Query: 241 EAAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPI 300
              +P   L  T +FRFLD+A I   DT       ++ PWRLCTVTQVE+ K LI ++PI
Sbjct: 300 NDVVPSSTLPHTPRFRFLDKACIKIQDT-----NTKESPWRLCTVTQVEQVKTLISLVPI 359

Query: 301 ILSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
             STI   T +AQLQTFS+QQ  +M+++    F IP  S+  IP + + F +P Y+   V
Sbjct: 360 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 419

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+TG  +GI  L RIGIGL L+  SM  A  +E +R++ ++    +       +S+
Sbjct: 420 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSI 479

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
           FW+  QF +FG+ ++FT VGL+EFFY +S+ GM+S   A+ +C+ +FG++ S+V+V+VVN
Sbjct: 480 FWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVN 539

Query: 481 KA------SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASW 520
           K       S GWL  N+LN+D+LD FYWLL+VLS+LNF  YL  + W
Sbjct: 540 KITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of Chy3G064830 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.0e-99
Identity = 218/540 (40.37%), Postives = 321/540 (59.44%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +A V    ++++Y +  ++  LTK+  TLTNF GT+ L  LLG FI++++  RF T    
Sbjct: 42  LAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFA 101

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSE-CEAAGSSQEAFLYTGLYLIALGTSGV 120
           +     G  LLT+ A  P LRP PCK    G E C  A ++Q + LY  L L ALG+ G+
Sbjct: 102 SIIYQIGMTLLTISAIIPTLRPPPCK----GEEVCVVADTAQLSILYVALLLGALGSGGI 161

Query: 121 KAALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVV 180
           +  + A GADQFD+ DP++  K  ++FNW+ F +    ++ +T++VWI   VGW     +
Sbjct: 162 RPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGI 221

Query: 181 CSIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDK 240
            ++A+  +++    G   YRH VP GSP  RL+QV VA+ R RKL +  + + L+   + 
Sbjct: 222 PTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEI 281

Query: 241 EAAIPY-EVLEKTDQFRFLDRAAIIRNDTIASISTNQ-QGPWRLCTVTQVEETKILIRML 300
           +A I     L  T    FLD+AAI+  +   ++   Q    WRL TV +VEE K +IRM 
Sbjct: 282 DAPISLGGKLTHTKHMSFLDKAAIVTEED--NLKPGQIPNHWRLSTVHRVEELKSVIRMG 341

Query: 301 PIILSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERV 360
           PI  S I + T  AQ  TFS+QQ+ TM+      F+IP  S+ V   + M   I FY+RV
Sbjct: 342 PIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRV 401

Query: 361 FVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPL-P 420
           FV +ARK TG+  GI  L R+GIG V++  +  +AGFVE +RK+VAI+H ++D    + P
Sbjct: 402 FVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVP 461

Query: 421 ISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVT 480
           IS  WL  Q+ + G+ + F  +G LEFFY ++   M+S +TA+ W AI+ G + ST++VT
Sbjct: 462 ISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVT 521

Query: 481 VVNKAS-----GGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQN 531
           +V+K S       WL  NNLNR +L+YFYWL++VL  +N  +YL CA  Y YK V++H +
Sbjct: 522 LVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWCAKIYTYKPVQVHHS 575

BLAST of Chy3G064830 vs. ExPASy TrEMBL
Match: A0A0A0LLC0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360700 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.1e-266
Identity = 468/477 (98.11%), Postives = 474/477 (99.37%), Query Frame = 0

Query: 67  GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPAL 126
           GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPAL
Sbjct: 13  GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPAL 72

Query: 127 GADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSIAVLA 186
           GADQFDD+DPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSI+VLA
Sbjct: 73  GADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVLA 132

Query: 187 AIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKEAAIPYE 246
           AI VVFLGKSFYRHNVPQGSPILR LQVFVASIRNRKLPLP NANELHEIRDKEAAIPYE
Sbjct: 133 AIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKEAAIPYE 192

Query: 247 VLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTIFM 306
           +LEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTIFM
Sbjct: 193 ILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTIFM 252

Query: 307 ATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITG 366
           ATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITG
Sbjct: 253 ATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITG 312

Query: 367 IPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQFC 426
           IPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQFC
Sbjct: 313 IPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQFC 372

Query: 427 VFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGWLA 486
           VFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGWLA
Sbjct: 373 VFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGWLA 432

Query: 487 SNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIAREA 544
           SNNLN+DKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDA+EEKVD+AREA
Sbjct: 433 SNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMAREA 489

BLAST of Chy3G064830 vs. ExPASy TrEMBL
Match: A0A6J1CR11 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 6.3e-262
Identity = 469/543 (86.37%), Postives = 499/543 (91.90%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 37  MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 96

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  GY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 97  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 156

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 157 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 216

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S+AV+AAIV V  GK FYR+N P+GSPI R+ QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 217 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 276

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYE+LEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 277 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 336

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 337 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 396

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 397 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 456

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 457 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 516

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWL SNNLNRDKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK DIA
Sbjct: 517 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 576

Query: 541 REA 544
           R A
Sbjct: 577 RGA 578

BLAST of Chy3G064830 vs. ExPASy TrEMBL
Match: A0A6J1CSN2 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 6.3e-262
Identity = 469/543 (86.37%), Postives = 499/543 (91.90%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  GY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 120

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 121 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 180

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S+AV+AAIV V  GK FYR+N P+GSPI R+ QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 181 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 240

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYE+LEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 241 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 300

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 301 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 360

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 420

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 421 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWL SNNLNRDKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK DIA
Sbjct: 481 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 540

Query: 541 REA 544
           R A
Sbjct: 541 RGA 542

BLAST of Chy3G064830 vs. ExPASy TrEMBL
Match: A0A6J1GIY5 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.4e-253
Identity = 452/541 (83.55%), Postives = 490/541 (90.57%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFV++ALSL+TYF+GYMNFSLTKS TTLTNFMGTTFLLTL+GGF+S+TYLSRFKTC+LF
Sbjct: 34  MAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLF 93

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A FEL GYCLLT QAHF  LRP PCK A AGSEC+AA   QEA LY G+YLIALG SGVK
Sbjct: 94  ACFELLGYCLLTAQAHFRELRPEPCKGAAAGSECKAANGGQEAILYVGIYLIALGASGVK 153

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            ALPALGADQF++ DP+EAGK+SSFFNWFLFSLT GS VGLTLIVWINTEVGWDWAFVVC
Sbjct: 154 VALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVC 213

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           + AVLAAI+VV  GKSF+R+NVP+GSPILR+LQV VASIRNRKLPLP NA+ELHEIRDKE
Sbjct: 214 TAAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRDKE 273

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           A IP E+LEKTDQFRFL+RAAIIRNDT        + PWRLCTVTQVEETKILIRMLPII
Sbjct: 274 ADIPIEILEKTDQFRFLERAAIIRNDT--------RTPWRLCTVTQVEETKILIRMLPII 333

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMA+CMAQLQTFSIQQS+TMDS F+GFKIPGPS+PVIPLLFMFFFIPFYERVFVPL
Sbjct: 334 VSTIFMASCMAQLQTFSIQQSLTMDSNFIGFKIPGPSVPVIPLLFMFFFIPFYERVFVPL 393

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIG GL+LTAASMAIAGFVETRRKNVAI+HNMVDS EPLP+SVFW
Sbjct: 394 ARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSEEPLPMSVFW 453

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFCVFGM DIFT+VGLLEFFYAESSA MKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 454 LSFQFCVFGMADIFTMVGLLEFFYAESSAEMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 513

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWLASNNLNRD L+YFYWLLSVLSV+NFGFYLVCASWYRYKNVE+ Q    EE  DI 
Sbjct: 514 SGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVLQKQG-EEGFDIV 565

Query: 541 R 542
           +
Sbjct: 574 Q 565

BLAST of Chy3G064830 vs. ExPASy TrEMBL
Match: A0A6J1KKU7 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.3e-253
Identity = 454/543 (83.61%), Postives = 491/543 (90.42%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFV++ALSL+TYFSGYMNFSLTKS TT+TNFMGTTFLLTL+GGF+S+TYLSRFKTC+LF
Sbjct: 49  MAFVALALSLMTYFSGYMNFSLTKSATTMTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLF 108

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A FEL GY LL+ QAHF  LRP PCK A AGSEC+AA   QEA LY G+YLIALG SGVK
Sbjct: 109 ACFELLGYFLLSAQAHFRELRPEPCKGALAGSECKAANGGQEAILYVGIYLIALGASGVK 168

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            ALPALGADQF++ DP+EAGK+SSFFNWFLFSLT GS VGLTLIVWINTEVGWDWAFVVC
Sbjct: 169 VALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVC 228

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           +IAVLAAI+VV  GKSF+R+NVP+GSPILR+LQV VASI NRKLPLPENA+ELHEIRDKE
Sbjct: 229 TIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHNRKLPLPENADELHEIRDKE 288

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           A IP E+LEKTDQFRFL+RAAIIRNDT        +  WRLCTVTQVEETKILIRMLPII
Sbjct: 289 ADIPIEILEKTDQFRFLERAAIIRNDT--------RTLWRLCTVTQVEETKILIRMLPII 348

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQS+TMDS FLGFKIPGPS+PVIPLLFMFFFIPFYERVFVPL
Sbjct: 349 VSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPL 408

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIG GL+LTAASMAIAGFVETRRKNVAI+HNMVDS EPLP+SVFW
Sbjct: 409 ARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSEEPLPMSVFW 468

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFCVFGM DIFT+VGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 469 LSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 528

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWLASNNLNRD L+YFYWLLSVLSV+NFGFYLVCASWYRYKNVE+ Q    EE  DI 
Sbjct: 529 SGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVLQKQG-EEGFDIV 582

Query: 541 REA 544
           + A
Sbjct: 589 QGA 582

BLAST of Chy3G064830 vs. NCBI nr
Match: XP_011649593.2 (protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus])

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 533/543 (98.16%), Postives = 539/543 (99.26%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF
Sbjct: 25  MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 84

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           AFFEL GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK
Sbjct: 85  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 144

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           AALPALGADQFDD+DPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC
Sbjct: 145 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 204

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           SI+VLAAI VVFLGKSFYRHNVPQGSPILR LQVFVASIRNRKLPLP NANELHEIRDKE
Sbjct: 205 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKE 264

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AAIPYE+LEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 265 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 324

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL
Sbjct: 325 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 384

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW
Sbjct: 385 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 444

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA
Sbjct: 445 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 504

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWLASNNLN+DKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDA+EEKVD+A
Sbjct: 505 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVDMA 564

Query: 541 REA 543
           REA
Sbjct: 565 REA 567

BLAST of Chy3G064830 vs. NCBI nr
Match: XP_038885750.1 (protein NRT1/ PTR FAMILY 4.5-like [Benincasa hispida])

HSP 1 Score: 966 bits (2498), Expect = 0.0
Identity = 492/541 (90.94%), Postives = 512/541 (94.64%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYL RFKTC+LF
Sbjct: 61  MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLPRFKTCVLF 120

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A FEL GYCLLT QAHFPHLRPAPCK A A SECEAAGS QEA LY GLYLIALGTSGVK
Sbjct: 121 ASFELMGYCLLTAQAHFPHLRPAPCKGAAARSECEAAGSGQEAILYVGLYLIALGTSGVK 180

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           AALPALGADQFDDRDP+EA KVSSFFNWFLFSLTIGSIVG+TLIVWINTEVGWDWAFVVC
Sbjct: 181 AALPALGADQFDDRDPAEAAKVSSFFNWFLFSLTIGSIVGVTLIVWINTEVGWDWAFVVC 240

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           SIA+L A+VVVF GKSFYR+NVP+GSPILRLLQVFVASIRNRKLPLPEN NELHEIRDKE
Sbjct: 241 SIAILVAVVVVFAGKSFYRNNVPKGSPILRLLQVFVASIRNRKLPLPENVNELHEIRDKE 300

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AAIP+E+LEKTDQFRFLDRAAI+RNDT AS+S NQ GPWRLCTVTQVEETKILIRMLPII
Sbjct: 301 AAIPHEILEKTDQFRFLDRAAIVRNDTNASMSANQPGPWRLCTVTQVEETKILIRMLPII 360

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMD QFLGFKIPGPS+PVIPLLFM FFIPFYE VFVPL
Sbjct: 361 VSTIFMATCMAQLQTFSIQQSITMDCQFLGFKIPGPSVPVIPLLFMIFFIPFYEHVFVPL 420

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLV+T AS+AIA  VETRRKN+AIKHNMVDS EPLP++VFW
Sbjct: 421 ARKITGIPTGIRHLQRIGIGLVITTASIAIAAVVETRRKNIAIKHNMVDSEEPLPMTVFW 480

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 481 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 540

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWLASNNLNRD LDYFYWLLSVLSVLNFGFYLVCASWY YKNV + QNDAMEEKVDIA
Sbjct: 541 SGGWLASNNLNRDNLDYFYWLLSVLSVLNFGFYLVCASWYTYKNVGVLQNDAMEEKVDIA 600

BLAST of Chy3G064830 vs. NCBI nr
Match: KAE8652098.1 (hypothetical protein Csa_022418 [Cucumis sativus])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 468/485 (96.49%), Postives = 474/485 (97.73%), Query Frame = 0

Query: 67  GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPAL 126
           GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPAL
Sbjct: 71  GYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPAL 130

Query: 127 GADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSIAVLA 186
           GADQFDD+DPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSI+VLA
Sbjct: 131 GADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVLA 190

Query: 187 AIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKEAAIPYE 246
           AI VVFLGKSFYRHNVPQGSPILR LQVFVASIRNRKLPLP NANELHEIRDKEAAIPYE
Sbjct: 191 AIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKEAAIPYE 250

Query: 247 VLEKTDQF--------RFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 306
           +LEKTDQF        RFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP
Sbjct: 251 ILEKTDQFSHGILELCRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 310

Query: 307 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 366
           IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV
Sbjct: 311 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 370

Query: 367 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 426
           PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV
Sbjct: 371 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 430

Query: 427 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 486
           FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN
Sbjct: 431 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 490

Query: 487 KASGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVD 543
           KASGGWLASNNLN+DKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDA+EEKVD
Sbjct: 491 KASGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDALEEKVD 550

BLAST of Chy3G064830 vs. NCBI nr
Match: XP_022144216.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Momordica charantia])

HSP 1 Score: 911 bits (2354), Expect = 0.0
Identity = 469/543 (86.37%), Postives = 499/543 (91.90%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  GY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 120

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 121 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 180

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S+AV+AAIV V  GK FYR+N P+GSPI R+ QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 181 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 240

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYE+LEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 241 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 300

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 301 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 360

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 420

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 421 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWL SNNLNRDKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK DIA
Sbjct: 481 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 540

Query: 541 REA 543
           R A
Sbjct: 541 RGA 542

BLAST of Chy3G064830 vs. NCBI nr
Match: XP_022144215.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 [Momordica charantia])

HSP 1 Score: 911 bits (2354), Expect = 0.0
Identity = 469/543 (86.37%), Postives = 499/543 (91.90%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTLLGGFIS+TYLSRFKTCL+F
Sbjct: 37  MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 96

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  GY LLT QAHF  LRPAPCK A A  ECEAA   QEA LY G+YLIALGTSGVK
Sbjct: 97  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 156

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
           +ALPALGADQFD  DP+EAGK+SSFFNWFLFSLT GSIVGLTLIVWINT+VGWDWAFVVC
Sbjct: 157 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 216

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           S+AV+AAIV V  GK FYR+N P+GSPI R+ QVFVASIRNRKLPLP+NA+ELHEIRDKE
Sbjct: 217 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 276

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYE+LEKTDQFRFLDRAAIIRN T AS++  QQGPWRLCTVTQVEETKILIRMLPII
Sbjct: 277 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 336

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDS FLGFKIPGPS+PVIPLLFM F IPFYERVFVPL
Sbjct: 337 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 396

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKN+AI+H+MVDS EPLP+SVFW
Sbjct: 397 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 456

Query: 421 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 480
           L FQFC+FGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 457 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 516

Query: 481 SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEIHQNDAMEEKVDIA 540
           SGGWL SNNLNRDKL+YFYWLLSVLSVLNFGFYL+CASWYRYKNVEI QND  EEK DIA
Sbjct: 517 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEILQNDTEEEKDDIA 576

Query: 541 REA 543
           R A
Sbjct: 577 RGA 578

BLAST of Chy3G064830 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 402.9 bits (1034), Expect = 4.1e-112
Identity = 219/529 (41.40%), Postives = 329/529 (62.19%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL LLGGF+++ + S F   L+ 
Sbjct: 42  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 101

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  G  LLT+QA  P L P PCK + A   CE  G S+ AFL+ GLYL++LG  G+K
Sbjct: 102 ASIEFLGLILLTIQARRPSLMPPPCKSS-AALRCEVVGGSKAAFLFVGLYLVSLGIGGIK 161

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V +T +VWI    GW+W F V 
Sbjct: 162 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 221

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFV-ASIRNRKLPLPENANELHEIR-D 240
           +I++  +I+V  LG  FY++ +P+GSP+  + +V + ASI +       N     E++ +
Sbjct: 222 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 281

Query: 241 KEAAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          I       W  CTV QVE+ KI+++MLP
Sbjct: 282 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 341

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
           I   TI +  C+AQL T+S+ Q+ TM+ + + F +P  S+PV P++FM    P Y+ + +
Sbjct: 342 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 401

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A  VE +RK VA +  ++DS E LPI+ 
Sbjct: 402 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 461

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W ++A GY+ S+V+V +VN
Sbjct: 462 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 521

Query: 481 KAS-----GGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRY 523
           + +       WL    LNR++LD FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 522 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of Chy3G064830 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 402.9 bits (1034), Expect = 4.1e-112
Identity = 219/529 (41.40%), Postives = 329/529 (62.19%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL LLGGF+++ + S F   L+ 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  G  LLT+QA  P L P PCK + A   CE  G S+ AFL+ GLYL++LG  G+K
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSS-AALRCEVVGGSKAAFLFVGLYLVSLGIGGIK 165

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V +T +VWI    GW+W F V 
Sbjct: 166 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 225

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFV-ASIRNRKLPLPENANELHEIR-D 240
           +I++  +I+V  LG  FY++ +P+GSP+  + +V + ASI +       N     E++ +
Sbjct: 226 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 285

Query: 241 KEAAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          I       W  CTV QVE+ KI+++MLP
Sbjct: 286 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 345

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
           I   TI +  C+AQL T+S+ Q+ TM+ + + F +P  S+PV P++FM    P Y+ + +
Sbjct: 346 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 405

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A  VE +RK VA +  ++DS E LPI+ 
Sbjct: 406 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 465

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W ++A GY+ S+V+V +VN
Sbjct: 466 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 525

Query: 481 KAS-----GGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRY 523
           + +       WL    LNR++LD FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 526 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Chy3G064830 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 398.7 bits (1023), Expect = 7.7e-111
Identity = 217/541 (40.11%), Postives = 332/541 (61.37%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           +A+++ A +LV Y   YM+ S +KS   +TNFMGT FLL LLGGF+S+ + S F+  L+ 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
           A  E  G  +LT+QA  P L P  C ++P    CE    S+ A L+ GLYL+ALG  G+K
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSC-DSPT---CEEVSGSKAAMLFVGLYLVALGVGGIK 163

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
            +L + GA+QFD+  P    + S+FFN+F+F L  G++V +T +VW+    GW+W F V 
Sbjct: 164 GSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVS 223

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVA---------SIRNRKLPLPENAN 240
           +IA+  +I++   G  FYR+ +P GSP+  +L+V +A         S  N    +  + +
Sbjct: 224 TIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS 283

Query: 241 ELHEIRDKEAAIPYEVLEKTDQFRFL-DRAAIIRNDTIASISTNQQGPWRL--CTVTQVE 300
                + K+       LEK  Q   L  RA +  +  + + + +++   RL  CTV QVE
Sbjct: 284 NHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVE 343

Query: 301 ETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFF 360
           + KI+++MLPI   TI +  C+AQL TFS+QQ+ +M+++    KIP  S+P+ P++F+  
Sbjct: 344 DVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMI 403

Query: 361 FIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMV 420
             P Y+ + +P ARK T   TG+ HLQRIG+GLVL+  +MA+A  VE +RK VA    ++
Sbjct: 404 LAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLL 463

Query: 421 DSTEPLPISVFWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYF 480
           DS E LP++  W+  Q+   G  D+FTL GLLE+F+ E+ + M+SL+T+++W ++A GY+
Sbjct: 464 DSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYY 523

Query: 481 TSTVVVTVVNKASGG-----WLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWYRYK 525
            S+V+V++VN  +G      WL   ++NR KLDYFYWL+ VLS  NF  YL  A  Y+Y+
Sbjct: 524 LSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 580

BLAST of Chy3G064830 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 397.9 bits (1021), Expect = 1.3e-110
Identity = 226/549 (41.17%), Postives = 334/549 (60.84%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           MA  +V  +L+TY    M+F L+KS   +TNF+GT FLL+LLGGF+S++YL  F+T L+F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
              E++G+ LL+VQAH P LRP  C        C  A   + A LYT L L+ALG+  +K
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + + GA+QF  +D     K+SSFFN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELHEIRDKE 240
           +  + A ++ +  G SFYR+  P GS    + QVFVA+I  RK   P N N +H+     
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQ--PST 296

Query: 241 AAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 300
             +  + L  +++FRFLD+A       I +     + PWRLCT+ QV + KIL+ ++PI 
Sbjct: 297 DLVRVKPLLHSNKFRFLDKAC------IKTQGKAMESPWRLCTIEQVHQVKILLSVIPIF 356

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFVP 360
             TI   T +AQLQTFS+QQ  +M++     F+IP  S+  IP + + FF+P YE  FVP
Sbjct: 357 ACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVP 416

Query: 361 LARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVF 420
           LARK+TG  +GI  LQRIG GL L   SM  A  VE +R+   ++ N++       +S+F
Sbjct: 417 LARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM-------LSIF 476

Query: 421 WLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNK 480
           W+  QF +FG+ ++FT VGL+EFFY +SS  M+S  TA+ +C+ +FG++ S+V+V+ VN+
Sbjct: 477 WIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNR 536

Query: 481 ASG--------GWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASWY------RYKNV 535
            +         GWL  N+LN+D+LD+FYWLL+ LS +NF  YL  + WY       + + 
Sbjct: 537 VTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSATHHSA 587

BLAST of Chy3G064830 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 5.7e-106
Identity = 216/527 (40.99%), Postives = 319/527 (60.53%), Query Frame = 0

Query: 1   MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 60
           M   +V  +L+TY    M+F L+K+   +TNF+GT F+  LLGG++S+ +L  F T ++F
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  AFFELTGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 120
            F EL+G+ LL+VQAH P L+P  C        CE A   +    +  LYL+ALG+  VK
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKCNPL-IDQTCEEAKGFKAMIFFMALYLVALGSGCVK 179

Query: 121 AALPALGADQFDDRDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 180
             + A GADQF    P ++ ++SS+FN   F+ ++G ++ LTL+VW+ T  G D  F V 
Sbjct: 180 PNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVS 239

Query: 181 SIAVLAAIVVVFLGKSFYRHNVPQGSPILRLLQVFVASIRNRKLPLPENANELH-EIRDK 240
           + A+   I+ +  G  ++R+  P+ S    +  V VA+I  RKL  P +   LH +    
Sbjct: 240 AAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVA 299

Query: 241 EAAIPYEVLEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPI 300
              +P   L  T +FRFLD+A I   DT       ++ PWRLCTVTQVE+ K LI ++PI
Sbjct: 300 NDVVPSSTLPHTPRFRFLDKACIKIQDT-----NTKESPWRLCTVTQVEQVKTLISLVPI 359

Query: 301 ILSTIFMATCMAQLQTFSIQQSITMDSQFL-GFKIPGPSIPVIPLLFMFFFIPFYERVFV 360
             STI   T +AQLQTFS+QQ  +M+++    F IP  S+  IP + + F +P Y+   V
Sbjct: 360 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 419

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISV 420
           P ARK+TG  +GI  L RIGIGL L+  SM  A  +E +R++ ++    +       +S+
Sbjct: 420 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSI 479

Query: 421 FWLGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVN 480
           FW+  QF +FG+ ++FT VGL+EFFY +S+ GM+S   A+ +C+ +FG++ S+V+V+VVN
Sbjct: 480 FWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVN 539

Query: 481 KA------SGGWLASNNLNRDKLDYFYWLLSVLSVLNFGFYLVCASW 520
           K       S GWL  N+LN+D+LD FYWLL+VLS+LNF  YL  + W
Sbjct: 540 KITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYE45.7e-11141.40Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q8H1571.1e-10940.11Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q56XQ61.8e-10941.17Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q93VV58.0e-10540.99Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q9SX201.0e-9940.37Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LLC01.1e-26698.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360700 PE=3 SV=1[more]
A0A6J1CR116.3e-26286.37protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1CSN26.3e-26286.37protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1GIY51.4e-25383.55protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 ... [more]
A0A6J1KKU75.3e-25383.61protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE... [more]
Match NameE-valueIdentityDescription
XP_011649593.20.098.16protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus][more]
XP_038885750.10.090.94protein NRT1/ PTR FAMILY 4.5-like [Benincasa hispida][more]
KAE8652098.10.096.49hypothetical protein Csa_022418 [Cucumis sativus][more]
XP_022144216.10.086.37protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Momordica charantia][more]
XP_022144215.10.086.37protein NRT1/ PTR FAMILY 4.5-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G27040.24.1e-11241.40Major facilitator superfamily protein [more]
AT1G27040.14.1e-11241.40Major facilitator superfamily protein [more]
AT1G69850.17.7e-11140.11nitrate transporter 1:2 [more]
AT1G33440.11.3e-11041.17Major facilitator superfamily protein [more]
AT1G59740.15.7e-10640.99Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..530
e-value: 5.9E-163
score: 544.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 6..524
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 54..481
e-value: 7.6E-94
score: 314.7
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..529
NoneNo IPR availablePANTHERPTHR11654:SF110PEPTIDE/NITRATE TRANSPORTERcoord: 1..529

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G064830.1Chy3G064830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042981 regulation of apoptotic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity