Chy3G064820 (gene) Cucumber (hystrix) v1

Overview
NameChy3G064820
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionThylakoidal processing peptidase 1
LocationchrH03: 17385081 .. 17387631 (+)
RNA-Seq ExpressionChy3G064820
SyntenyChy3G064820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTCGTGTTACTCTCTCCTACTCTGGTCATGTCGTCCAAAACCTAGCCTCCTCCACCGGCCTTCGGGCCGGTAACTGCCGCGTCTTTCAGGAGTTTTGGGTCCGATCTTGTATTTTTGGCTCGACCCATAATCCGGAGCTCAAATCTTCTGGAAGCGCGCGCAATTATCGGTCCGAAAGCCGGCGGTTCAAGCCGGGTGGCTCTGTTGAGAAGGCAACTGCTATGTACAGTACTCTCGCCGGAGAAAGGGTTGGGGAAAGCCCTAAGAATCCGATGATTTTGGGTTTGATGTCGATGTTGAAATCGATGGGTGATTCTTCTGTGATCAGTACGGGGACTTCTGGGGTTTCTTCGTTTAAAGCTACTTCGACTATACCCTTTTTACAAGGATCGAAGTGGCTTCCGGGTTATGATGTACGATCTGTGAGCGACGATGTGGATAAAGGCGGAACAACTGTTTGTTATGATTATTATGATAAAAGTGGAAATAATCAGTTTTATGAGAATGATTTTGAGAAGAGCAGCTGGGTTTCGCGCTTGTTGAGTACTTATTCAGAGGATGCAAAAGCCCTCTTTACGGCTTTGACTGTCAGTGTGCTCTTTAAATCTTTCTTGGCAGAGCCGAAATCGATTCCTTCTTCTTCCATGTGTCCAACTCTGGAAGTAGGGGATCGTATTTTAGCAGAAAAGGTGGGACTTTAGTTAATCAAATTGAAAATTTCTGAATTGACTTGTTGATGAACATTAATTTATTATCTAACATTACTGAGATCTGGATTAAATTCTTCTTTTATGACTATGATTACCTTAATTAAACTTTTGAACGTAACGGCCTCCTAACAATCGTAATTCAAAAAAAAGAAAAAAGCTTATTTCTTGTTTTAGTTTATATGGCCGTGGAAGTGTCTTACCCTGTTCCTAAACTGATCGATTGCTTTTCCTTCTTATATTAGGTTTCGTACCTTTTCAGGAAACCTGAAGTTTCTGATATAGTGATCTTTAAAGCTCCTCAAATCTTGCAGGTATTACTTTAATTAATAAGGAAATTTTGAGGAAATTTTCCTGTTTTTGTAATGTTGGATGCTGATGTTTTCTTGTGCCTTTTTGCAATTCATTTAGGATTTTGGAGTTAGTTCAGATGAGGTGTTTATTAAGAGAGTTGTGGCTACATCTGGGGACGTGGTTGAAGTAAGTGTCCTTCAAAATTTTAACTTTTCGTTTCTTTATTACGCTATAATTCTATGCTTTCTCTGCATTCCAAAATAATAATTATTATAATAAATAAATAAATAAACAAATAAATTTTATTATTTGTTCCTTTGATTCATATTAGTAAGCAAATAAGTAAAACAATTTATTAACCTGAATTTCAAGTAATATCAGATACTTAAATCCACTAGATTATGGAGAATTATGAAGATAAGTGAGGATTTTAAAATACCTTGCACTAGCATCATCGGATGGAATATTTTGGAGTCATTAGTCACTTAGTTTTTCTTTCAAATAATTTGGTAGCTCAGCACTGAATATTTTGATCGGAGTATTCCATAAGCATCTGAGATTTGACCTTTAGTCGTCTTGTTTTTAACTTCTGACCAAATTAGTGTGGTTCACGCTAACCACTTGGCATTGGAATGTCTAATTGTAGGTTCAAAAAGGGAAATTGGTGGTAAATGGTGTAGCTCAGGATGAGGACTTCGTGTTAGAGCCAATTGCTTATGACATGGAACCGTTGGTAATTGTCTTTCCTTTCCAACCTTCATTCCCTCTCTCTGCCTCTTTCTCTCTCCCTCTCCAGAGGGAGATTGAAAATCCTGAATTCTGTTCTAAGGATAGACTGTTTGCTCAATCCAATTATCTTGTTTTCTTCTACAGCTAGTGCCTGAAGGTTATGTGTATGTAATGGGAGACAACCGTAACAATAGTTGTGATTCTCATAACTGGTAAGTTCACCATCTCAACTTTCATGTTGTATAATGTGAGAGCACCATTAGTATTAGGGAATATATAAGGAATATTAGTTTAATATATGCAACGATAGCGGTGATTTGAGGGAGTTTGAGTTCTTAGAAGTGGGATCATTATAGTGAAATCAGGATTGTCCATATTTGGAGTTTGTGAGGGAAATAGGCTAATAGCTCTCTTGTAGGCTATTGTTATATCGTGATTTCTTTTGATAACACAGTATAGGGCTATCTTGTGTTTTCTCTGTATTTTAATGCCTACCATCTAATTGGAACTTGTGGTATCAACTTTTGGCACACACATGTTTTATGTTATACTATACACGCCGTAACTCTTTTAATGGTAGATGAGAATCGACTCTCCATGATGCTTTCTTTTTTCTTTTTAAAGAGAATATGGTCGATGTTAGCCACCAATCTTTCTGATGATATGGTATACTGACTATTTCTTCTTCTCCAGGGGTCCACTCCCAATTGAAAATATTGTAGGTAGATCATTATTCAAGTATTGGCCTCCTTCCAAGGGATCCGCTATGGTAGATGAACTACGTGTTGGGAAGATTAATCTCGGGATTTCCTGA

mRNA sequence

ATGGCTTTTCGTGTTACTCTCTCCTACTCTGGTCATGTCGTCCAAAACCTAGCCTCCTCCACCGGCCTTCGGGCCGGTAACTGCCGCGTCTTTCAGGAGTTTTGGGTCCGATCTTGTATTTTTGGCTCGACCCATAATCCGGAGCTCAAATCTTCTGGAAGCGCGCGCAATTATCGGTCCGAAAGCCGGCGGTTCAAGCCGGGTGGCTCTGTTGAGAAGGCAACTGCTATGTACAGTACTCTCGCCGGAGAAAGGGTTGGGGAAAGCCCTAAGAATCCGATGATTTTGGGTTTGATGTCGATGTTGAAATCGATGGGTGATTCTTCTGTGATCAGTACGGGGACTTCTGGGGTTTCTTCGTTTAAAGCTACTTCGACTATACCCTTTTTACAAGGATCGAAGTGGCTTCCGGGTTATGATGTACGATCTGTGAGCGACGATGTGGATAAAGGCGGAACAACTGTTTGTTATGATTATTATGATAAAAGTGGAAATAATCAGTTTTATGAGAATGATTTTGAGAAGAGCAGCTGGGTTTCGCGCTTGTTGAGTACTTATTCAGAGGATGCAAAAGCCCTCTTTACGGCTTTGACTGTCAGTGTGCTCTTTAAATCTTTCTTGGCAGAGCCGAAATCGATTCCTTCTTCTTCCATGTGTCCAACTCTGGAAGTAGGGGATCGTATTTTAGCAGAAAAGGTTTCGTACCTTTTCAGGAAACCTGAAGTTTCTGATATAGTGATCTTTAAAGCTCCTCAAATCTTGCAGGATTTTGGAGTTAGTTCAGATGAGGTGTTTATTAAGAGAGTTGTGGCTACATCTGGGGACGTGGTTGAAGTTCAAAAAGGGAAATTGGTGGTAAATGGTGTAGCTCAGGATGAGGACTTCGTGTTAGAGCCAATTGCTTATGACATGGAACCGTTGCTAGTGCCTGAAGGTTATGTGTATGTAATGGGAGACAACCGTAACAATAGTTGTGATTCTCATAACTGGGGTCCACTCCCAATTGAAAATATTGTAGGTAGATCATTATTCAAGTATTGGCCTCCTTCCAAGGGATCCGCTATGGTAGATGAACTACGTGTTGGGAAGATTAATCTCGGGATTTCCTGA

Coding sequence (CDS)

ATGGCTTTTCGTGTTACTCTCTCCTACTCTGGTCATGTCGTCCAAAACCTAGCCTCCTCCACCGGCCTTCGGGCCGGTAACTGCCGCGTCTTTCAGGAGTTTTGGGTCCGATCTTGTATTTTTGGCTCGACCCATAATCCGGAGCTCAAATCTTCTGGAAGCGCGCGCAATTATCGGTCCGAAAGCCGGCGGTTCAAGCCGGGTGGCTCTGTTGAGAAGGCAACTGCTATGTACAGTACTCTCGCCGGAGAAAGGGTTGGGGAAAGCCCTAAGAATCCGATGATTTTGGGTTTGATGTCGATGTTGAAATCGATGGGTGATTCTTCTGTGATCAGTACGGGGACTTCTGGGGTTTCTTCGTTTAAAGCTACTTCGACTATACCCTTTTTACAAGGATCGAAGTGGCTTCCGGGTTATGATGTACGATCTGTGAGCGACGATGTGGATAAAGGCGGAACAACTGTTTGTTATGATTATTATGATAAAAGTGGAAATAATCAGTTTTATGAGAATGATTTTGAGAAGAGCAGCTGGGTTTCGCGCTTGTTGAGTACTTATTCAGAGGATGCAAAAGCCCTCTTTACGGCTTTGACTGTCAGTGTGCTCTTTAAATCTTTCTTGGCAGAGCCGAAATCGATTCCTTCTTCTTCCATGTGTCCAACTCTGGAAGTAGGGGATCGTATTTTAGCAGAAAAGGTTTCGTACCTTTTCAGGAAACCTGAAGTTTCTGATATAGTGATCTTTAAAGCTCCTCAAATCTTGCAGGATTTTGGAGTTAGTTCAGATGAGGTGTTTATTAAGAGAGTTGTGGCTACATCTGGGGACGTGGTTGAAGTTCAAAAAGGGAAATTGGTGGTAAATGGTGTAGCTCAGGATGAGGACTTCGTGTTAGAGCCAATTGCTTATGACATGGAACCGTTGCTAGTGCCTGAAGGTTATGTGTATGTAATGGGAGACAACCGTAACAATAGTTGTGATTCTCATAACTGGGGTCCACTCCCAATTGAAAATATTGTAGGTAGATCATTATTCAAGTATTGGCCTCCTTCCAAGGGATCCGCTATGGTAGATGAACTACGTGTTGGGAAGATTAATCTCGGGATTTCCTGA

Protein sequence

MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRSESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSSFKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELRVGKINLGIS*
Homology
BLAST of Chy3G064820 vs. ExPASy Swiss-Prot
Match: Q9M9Z2 (Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP2 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 9.7e-94
Identity = 195/374 (52.14%), Postives = 251/374 (67.11%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLR--AGNCRVFQEFWVRSCIFGSTHNPEL--KSSGSAR 60
           MA RVT +YS +V +++ASS G R   G+ R   E WVR    G    P++  KS GS  
Sbjct: 1   MAIRVTFTYSSYVARSIASSAGTRVGTGDVRSCFETWVRPRFCGHNQIPDIVDKSPGSNT 60

Query: 61  NYRSESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTS 120
              S   R +P      A++MYST+A E + E  K+P++LG++S++   G          
Sbjct: 61  WGPSSGPRARP------ASSMYSTIAREILEEGCKSPLVLGMISLMNLTGAPQFSGMTGL 120

Query: 121 GVSSFKATSTIPFLQGSKWLPGYDVRSVSDD---VDKGGTT----VCYDYYDKSGNNQFY 180
           G+S FK +S IPFL+GSKW+P     ++S D   VD+GG      V  +  DK  N    
Sbjct: 121 GISPFKTSSVIPFLRGSKWMPCSIPATLSTDIAEVDRGGKVCDPKVKLELSDKVSNG--- 180

Query: 181 ENDFEKSSWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRIL 240
                 + WV++LL+  SEDAKA FTA+TVS+LF+S LAEPKSIPS+SM PTL+VGDR++
Sbjct: 181 -----GNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDRVI 240

Query: 241 AEKVSYLFRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGV 300
           AEKVSY FRKPEVSDIVIFKAP IL + G S  +VFIKR+VA+ GD VEV  GKL+VN  
Sbjct: 241 AEKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVNDT 300

Query: 301 AQDEDFVLEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPP 360
            Q EDFVLEPI Y+MEP+ VPEGYV+V+GDNRN S DSHNWGPLPI+NI+GRS+F+YWPP
Sbjct: 301 VQAEDFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRYWPP 360

Query: 361 SKGSAMVDELRVGK 364
           SK S ++   +V +
Sbjct: 361 SKVSDIIHHEQVSQ 360

BLAST of Chy3G064820 vs. ExPASy Swiss-Prot
Match: O04348 (Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TPP1 PE=2 SV=2)

HSP 1 Score: 344.7 bits (883), Expect = 1.3e-93
Identity = 196/356 (55.06%), Postives = 243/356 (68.26%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA R+T +YS HV +NL    G R G      E  VR   F  +H  +     S RN   
Sbjct: 1   MAIRITFTYSTHVARNL---VGTRVGPGGYCFESLVRPRFF--SHKRDF--DRSPRN--- 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
                       +  +MY ++A E +GE  ++P+++GL+S+LKS       +    GVSS
Sbjct: 61  ------------RPASMYGSIARELIGEGSQSPLVMGLISILKSTTGHESSTMNVLGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKA+S IPFLQGSKW+       V DDVDKGG TVC D  DK   N         S WV+
Sbjct: 121 FKASSIIPFLQGSKWIKN---PPVIDDVDKGG-TVCDDDDDKESRN-------GGSGWVN 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           +LLS  SEDAKA FTA+TVS+LF+S LAEPKSIPS+SM PTL+ GDR++AEKVSY FRKP
Sbjct: 181 KLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFFRKP 240

Query: 241 EVSDIVIFKAPQIL---QDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVL 300
           EVSDIVIFKAP IL    ++G SS++VFIKR+VA+ GD VEV+ GKL VN + Q+EDFVL
Sbjct: 241 EVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQEEDFVL 300

Query: 301 EPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGS 354
           EP++Y+MEP+ VP+GYV+V+GDNRN S DSHNWGPLPIENIVGRS+F+YWPPSK S
Sbjct: 301 EPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWGPLPIENIVGRSVFRYWPPSKVS 323

BLAST of Chy3G064820 vs. ExPASy Swiss-Prot
Match: Q8H0W1 (Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 SV=2)

HSP 1 Score: 227.3 bits (578), Expect = 2.9e-58
Identity = 108/185 (58.38%), Postives = 142/185 (76.76%), Query Frame = 0

Query: 174 EKSSWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKV 233
           EK+      L   S+DA+ +F A+ VS+ F+ F+AEP+ IPS SM PT +VGDR++AEKV
Sbjct: 99  EKNRLFPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKV 158

Query: 234 SYLFRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDE 293
           SY FRKP  +DIVIFK+P +LQ+ G +  +VFIKR+VA  GD+VEV  GKL+VNGVA++E
Sbjct: 159 SYYFRKPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNE 218

Query: 294 DFVLEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGS 353
            F+LEP  Y+M P+ VPE  V+VMGDNRNNS DSH WGPLP++NI+GRS+F+YWPP++ S
Sbjct: 219 KFILEPPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRVS 278

Query: 354 AMVDE 359
             V E
Sbjct: 279 GTVLE 283

BLAST of Chy3G064820 vs. ExPASy Swiss-Prot
Match: P72660 (Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB1 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.6e-40
Identity = 80/164 (48.78%), Postives = 113/164 (68.90%), Query Frame = 0

Query: 188 EDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKPEVSDIVI 247
           E+   L  AL +++L + F+AEP+ IPS SM PTLE GDR++ EKVSY F  P+V DI++
Sbjct: 15  ENIPLLMVALVLALLLRFFVAEPRYIPSDSMLPTLEQGDRLVVEKVSYHFHPPQVGDIIV 74

Query: 248 FKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPIAYDMEPL 307
           F  P++LQ  G    + FIKRV+A  G  VEV  G +  +G    E+++LEP  Y++  +
Sbjct: 75  FHPPELLQVQGYDLGQAFIKRVIALPGQTVEVNNGIVYRDGQPLQEEYILEPPQYNLPAV 134

Query: 308 LVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSK 352
            VP+G V+VMGDNRNNS DSH WG LP +NI+G +LF+++P S+
Sbjct: 135 RVPDGQVFVMGDNRNNSNDSHVWGFLPQQNIIGHALFRFFPASR 178

BLAST of Chy3G064820 vs. ExPASy Swiss-Prot
Match: P73157 (Probable signal peptidase I-2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=lepB2 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 3.9e-34
Identity = 70/175 (40.00%), Postives = 111/175 (63.43%), Query Frame = 0

Query: 184 STYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKPEVS 243
           +T+ E  K + TA+ +++  ++F+AE + IPSSSM PTL++ DR++ EK+SY  R PE  
Sbjct: 19  NTWLELGKTMVTAVILAIGIRTFVAEARYIPSSSMEPTLQINDRLIIEKISYRLRDPERG 78

Query: 244 DIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPIAYD 303
           +IV+F     L+    +  + FIKR++   GD V V +G + VNG   DE+++  P AY+
Sbjct: 79  EIVVFNPTDALK--AKNFHDAFIKRIIGLPGDEVRVSQGNVYVNGKMLDENYIAAPPAYE 138

Query: 304 MEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDE 359
             P+ VP+    V+GDNRNNS DSH WG +P E ++GR+  ++WP  +   + D+
Sbjct: 139 YGPVKVPDDQYLVLGDNRNNSYDSHYWGFVPREKLLGRAFVRFWPVPRVGLLTDD 191

BLAST of Chy3G064820 vs. ExPASy TrEMBL
Match: A0A0A0LNI7 (Peptidase_S26 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360710 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.7e-202
Identity = 361/369 (97.83%), Postives = 364/369 (98.64%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS
Sbjct: 1   MAIRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
           +SRRFKPGGSVEKATAMYSTL GERVGESPKNPMILGLMSMLKSMGDSSVISTG SGVSS
Sbjct: 61  DSRRFKPGGSVEKATAMYSTLTGERVGESPKNPMILGLMSMLKSMGDSSVISTGISGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKATS IPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGN+QFYENDFEK SWVS
Sbjct: 121 FKATSIIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNDQFYENDFEK-SWVS 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSY+FRKP
Sbjct: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYIFRKP 240

Query: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300
           EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI
Sbjct: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300

Query: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360
           AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR
Sbjct: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360

Query: 361 VGKINLGIS 370
           VGKINLGIS
Sbjct: 361 VGKINLGIS 368

BLAST of Chy3G064820 vs. ExPASy TrEMBL
Match: A0A1S3BBL0 (thylakoidal processing peptidase 1, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103487929 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.2e-195
Identity = 348/369 (94.31%), Postives = 357/369 (96.75%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSGHV QNLASSTGLRAGNCRVFQEF  RSCIF STH+P+LKSSGSARNYRS
Sbjct: 1   MAIRVTLSYSGHVAQNLASSTGLRAGNCRVFQEFRFRSCIFDSTHDPKLKSSGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
           +SRRFKPGGSVEK T+MYSTLAGERVGES KNPM+LGLMSMLKSMGDSS+ISTG  GVSS
Sbjct: 61  DSRRFKPGGSVEKPTSMYSTLAGERVGESSKNPMMLGLMSMLKSMGDSSMISTGNFGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKATS IPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS
Sbjct: 121 FKATSIIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           RLL+TYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSY FRKP
Sbjct: 181 RLLTTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYFFRKP 240

Query: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300
           EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGV QDE+FVLEPI
Sbjct: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVVQDEEFVLEPI 300

Query: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360
           AY+MEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR
Sbjct: 301 AYEMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360

Query: 361 VGKINLGIS 370
           VGKINLGIS
Sbjct: 361 VGKINLGIS 369

BLAST of Chy3G064820 vs. ExPASy TrEMBL
Match: A0A5A7VHF7 (Thylakoidal processing peptidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G006170 PE=4 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.7e-195
Identity = 347/369 (94.04%), Postives = 356/369 (96.48%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSGHV QNLASSTGLRAGNCRVFQEF  RSCIF STH+P+LKSSGSARNYRS
Sbjct: 1   MAIRVTLSYSGHVAQNLASSTGLRAGNCRVFQEFRFRSCIFDSTHDPKLKSSGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
           +SRRFKPGGSVEK T+MYSTLAGERVGES KNPM+LGLMSMLKSMGDSS+ISTG  GVSS
Sbjct: 61  DSRRFKPGGSVEKPTSMYSTLAGERVGESSKNPMMLGLMSMLKSMGDSSMISTGNFGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKATS IPFLQGSKWLPGYDVRSVS DVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS
Sbjct: 121 FKATSIIPFLQGSKWLPGYDVRSVSGDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           RLL+TYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSY FRKP
Sbjct: 181 RLLTTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYFFRKP 240

Query: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300
           EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGV QDE+FVLEPI
Sbjct: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVVQDEEFVLEPI 300

Query: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360
           AY+MEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR
Sbjct: 301 AYEMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360

Query: 361 VGKINLGIS 370
           VGKINLGIS
Sbjct: 361 VGKINLGIS 369

BLAST of Chy3G064820 vs. ExPASy TrEMBL
Match: A0A6J1CT20 (thylakoidal processing peptidase 1, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111013959 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 311/374 (83.16%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSG+V QNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKS+GSARNYRS
Sbjct: 1   MAIRVTLSYSGYVAQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSAGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGD---SSVISTGTSG 120
           +SRRFKP GS++K  +MYSTLAGE VGE+PK+P++LGLMSMLKS+     SS I+TG  G
Sbjct: 61  DSRRFKPSGSMKKPASMYSTLAGEMVGENPKSPVVLGLMSMLKSVASASGSSSITTGIFG 120

Query: 121 VSSFKATSTIPFLQGSKWLPGYDVRS-VSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKS 180
            SSFKATS IPFLQGSKWLPGYD+RS VSDDVDKGG T+  D YD  G+NQ YENDFEKS
Sbjct: 121 ASSFKATSIIPFLQGSKWLPGYDIRSHVSDDVDKGGGTIVSD-YDGIGSNQIYENDFEKS 180

Query: 181 SWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYL 240
           SWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSM PTLEVGDR+LAEKVSY 
Sbjct: 181 SWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMYPTLEVGDRVLAEKVSYF 240

Query: 241 FRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFV 300
           FRKPEVSDIV+FKAPQILQ+ GVSS EVFIKRVVATSGDVVEV KGKLVVNGV QDEDF+
Sbjct: 241 FRKPEVSDIVVFKAPQILQEIGVSSSEVFIKRVVATSGDVVEVLKGKLVVNGVVQDEDFI 300

Query: 301 LEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYW-PPSKGSAM 360
           LEPIAY+M+PL+VPEGYVYVMGDNRNNSCDSHNWGPL IENIVGRSLFKYW PP KGS+M
Sbjct: 301 LEPIAYEMDPLVVPEGYVYVMGDNRNNSCDSHNWGPLAIENIVGRSLFKYWPPPPKGSSM 360

Query: 361 VDELRVGKINLGIS 370
            D     KI LGIS
Sbjct: 361 ADSPHASKIKLGIS 373

BLAST of Chy3G064820 vs. ExPASy TrEMBL
Match: A0A6J1GIQ5 (thylakoidal processing peptidase 1, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454555 PE=4 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.4e-164
Identity = 301/373 (80.70%), Postives = 333/373 (89.28%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSG+V QNLASSTGLRAGNCRVFQE WVRSCIFGS+HNP+LKS+GSARNYRS
Sbjct: 1   MAIRVTLSYSGYVAQNLASSTGLRAGNCRVFQECWVRSCIFGSSHNPDLKSAGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSM---GDSSVISTGTSG 120
           +SRRFKP       ++MYS L GE VGE+PK+PMILGL+SMLKSM    +SS I TG  G
Sbjct: 61  DSRRFKP-----SCSSMYSALPGEMVGENPKSPMILGLISMLKSMASASESSPIPTGILG 120

Query: 121 VSSFKATSTIPFLQGSKWLPGYDVRS-VSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKS 180
           VSSFKATS IPFL+GS WLPGYD+RS VSDDVDKGG TVC D YD+SG+++FYE+DFEKS
Sbjct: 121 VSSFKATSIIPFLRGSNWLPGYDIRSHVSDDVDKGG-TVCCD-YDESGSDEFYESDFEKS 180

Query: 181 SWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYL 240
           SWVSRLL+TYS+DAKALFTALTVSVLFKSFLAEPKSIPSSSM PTLE GDR+LAEKVSY 
Sbjct: 181 SWVSRLLNTYSDDAKALFTALTVSVLFKSFLAEPKSIPSSSMYPTLEAGDRVLAEKVSYF 240

Query: 241 FRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFV 300
           FRKPEVSDIVIFK P+ILQ+FGVSS EVFIKRVVATSGDVVEV+ GKLVVNGV +DEDF+
Sbjct: 241 FRKPEVSDIVIFKPPKILQEFGVSSSEVFIKRVVATSGDVVEVRNGKLVVNGVVEDEDFI 300

Query: 301 LEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMV 360
           LEPIAY+M+P+LVPEGYVYVMGDNRNNSCDSHNWGPLP+ENIVGRSLFKYWPP KGS+MV
Sbjct: 301 LEPIAYEMDPMLVPEGYVYVMGDNRNNSCDSHNWGPLPVENIVGRSLFKYWPPPKGSSMV 360

Query: 361 DELRVGKINLGIS 370
           DE     INLG+S
Sbjct: 361 DEPHAKNINLGMS 366

BLAST of Chy3G064820 vs. NCBI nr
Match: XP_004152720.1 (thylakoidal processing peptidase 1, chloroplastic [Cucumis sativus] >KGN62554.1 hypothetical protein Csa_022323 [Cucumis sativus])

HSP 1 Score: 713 bits (1841), Expect = 7.15e-259
Identity = 361/369 (97.83%), Postives = 364/369 (98.64%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS
Sbjct: 1   MAIRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
           +SRRFKPGGSVEKATAMYSTL GERVGESPKNPMILGLMSMLKSMGDSSVISTG SGVSS
Sbjct: 61  DSRRFKPGGSVEKATAMYSTLTGERVGESPKNPMILGLMSMLKSMGDSSVISTGISGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKATS IPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGN+QFYENDFEKS WVS
Sbjct: 121 FKATSIIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNDQFYENDFEKS-WVS 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSY+FRKP
Sbjct: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYIFRKP 240

Query: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300
           EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI
Sbjct: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300

Query: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360
           AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR
Sbjct: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360

Query: 361 VGKINLGIS 369
           VGKINLGIS
Sbjct: 361 VGKINLGIS 368

BLAST of Chy3G064820 vs. NCBI nr
Match: XP_008444657.1 (PREDICTED: thylakoidal processing peptidase 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 691 bits (1783), Expect = 5.13e-250
Identity = 348/369 (94.31%), Postives = 357/369 (96.75%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSGHV QNLASSTGLRAGNCRVFQEF  RSCIF STH+P+LKSSGSARNYRS
Sbjct: 1   MAIRVTLSYSGHVAQNLASSTGLRAGNCRVFQEFRFRSCIFDSTHDPKLKSSGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
           +SRRFKPGGSVEK T+MYSTLAGERVGES KNPM+LGLMSMLKSMGDSS+ISTG  GVSS
Sbjct: 61  DSRRFKPGGSVEKPTSMYSTLAGERVGESSKNPMMLGLMSMLKSMGDSSMISTGNFGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKATS IPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS
Sbjct: 121 FKATSIIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           RLL+TYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSY FRKP
Sbjct: 181 RLLTTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYFFRKP 240

Query: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300
           EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGV QDE+FVLEPI
Sbjct: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVVQDEEFVLEPI 300

Query: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360
           AY+MEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR
Sbjct: 301 AYEMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360

Query: 361 VGKINLGIS 369
           VGKINLGIS
Sbjct: 361 VGKINLGIS 369

BLAST of Chy3G064820 vs. NCBI nr
Match: KAA0065275.1 (thylakoidal processing peptidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 688 bits (1776), Expect = 5.98e-249
Identity = 347/369 (94.04%), Postives = 356/369 (96.48%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSGHV QNLASSTGLRAGNCRVFQEF  RSCIF STH+P+LKSSGSARNYRS
Sbjct: 1   MAIRVTLSYSGHVAQNLASSTGLRAGNCRVFQEFRFRSCIFDSTHDPKLKSSGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
           +SRRFKPGGSVEK T+MYSTLAGERVGES KNPM+LGLMSMLKSMGDSS+ISTG  GVSS
Sbjct: 61  DSRRFKPGGSVEKPTSMYSTLAGERVGESSKNPMMLGLMSMLKSMGDSSMISTGNFGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKATS IPFLQGSKWLPGYDVRSVS DVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS
Sbjct: 121 FKATSIIPFLQGSKWLPGYDVRSVSGDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           RLL+TYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSY FRKP
Sbjct: 181 RLLTTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYFFRKP 240

Query: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPI 300
           EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGV QDE+FVLEPI
Sbjct: 241 EVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVVQDEEFVLEPI 300

Query: 301 AYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360
           AY+MEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR
Sbjct: 301 AYEMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDELR 360

Query: 361 VGKINLGIS 369
           VGKINLGIS
Sbjct: 361 VGKINLGIS 369

BLAST of Chy3G064820 vs. NCBI nr
Match: XP_038884798.1 (thylakoidal processing peptidase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 657 bits (1696), Expect = 9.27e-237
Identity = 332/370 (89.73%), Postives = 351/370 (94.86%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSG+VVQNLASSTGLRAGNCRVFQEFWVRSC+FGSTHNPE KS+GSARNYRS
Sbjct: 1   MAIRVTLSYSGYVVQNLASSTGLRAGNCRVFQEFWVRSCVFGSTHNPEFKSAGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
           +SRRFKPGGSVEK T+MYSTLAGERVGESPKNPM+LGLMSMLKSM DSS I+TGT GVSS
Sbjct: 61  DSRRFKPGGSVEKPTSMYSTLAGERVGESPKNPMVLGLMSMLKSMADSSAINTGTFGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRS-VSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWV 180
           FKATS I FLQGSKWLPGYD+RS VS++VDKGGTTVCYDY D+SG+N+FYENDFEKSSWV
Sbjct: 121 FKATSIISFLQGSKWLPGYDIRSNVSNNVDKGGTTVCYDY-DESGSNRFYENDFEKSSWV 180

Query: 181 SRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRK 240
           SRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSY FRK
Sbjct: 181 SRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYFFRK 240

Query: 241 PEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEP 300
           PEVSDIVIFKAPQILQ+FGVSS+E+FIKRVVATSGDVV V KGKLVVNGV QDEDFVLEP
Sbjct: 241 PEVSDIVIFKAPQILQEFGVSSNEMFIKRVVATSGDVVAVSKGKLVVNGVVQDEDFVLEP 300

Query: 301 IAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGSAMVDEL 360
           IAY+M+PLLVPEGYVYVMGDNRNNSCDSHNWGPL IENIVGRSLFKYWPPSKGS+MVDE 
Sbjct: 301 IAYEMDPLLVPEGYVYVMGDNRNNSCDSHNWGPLAIENIVGRSLFKYWPPSKGSSMVDEP 360

Query: 361 RVGKINLGIS 369
           R   INLGIS
Sbjct: 361 RARNINLGIS 369

BLAST of Chy3G064820 vs. NCBI nr
Match: XP_022144217.1 (thylakoidal processing peptidase 1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 604 bits (1558), Expect = 1.13e-215
Identity = 311/374 (83.16%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA RVTLSYSG+V QNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKS+GSARNYRS
Sbjct: 1   MAIRVTLSYSGYVAQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSAGSARNYRS 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGD---SSVISTGTSG 120
           +SRRFKP GS++K  +MYSTLAGE VGE+PK+P++LGLMSMLKS+     SS I+TG  G
Sbjct: 61  DSRRFKPSGSMKKPASMYSTLAGEMVGENPKSPVVLGLMSMLKSVASASGSSSITTGIFG 120

Query: 121 VSSFKATSTIPFLQGSKWLPGYDVRS-VSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKS 180
            SSFKATS IPFLQGSKWLPGYD+RS VSDDVDKGG T+  DY D  G+NQ YENDFEKS
Sbjct: 121 ASSFKATSIIPFLQGSKWLPGYDIRSHVSDDVDKGGGTIVSDY-DGIGSNQIYENDFEKS 180

Query: 181 SWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYL 240
           SWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSM PTLEVGDR+LAEKVSY 
Sbjct: 181 SWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMYPTLEVGDRVLAEKVSYF 240

Query: 241 FRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFV 300
           FRKPEVSDIV+FKAPQILQ+ GVSS EVFIKRVVATSGDVVEV KGKLVVNGV QDEDF+
Sbjct: 241 FRKPEVSDIVVFKAPQILQEIGVSSSEVFIKRVVATSGDVVEVLKGKLVVNGVVQDEDFI 300

Query: 301 LEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPS-KGSAM 360
           LEPIAY+M+PL+VPEGYVYVMGDNRNNSCDSHNWGPL IENIVGRSLFKYWPP  KGS+M
Sbjct: 301 LEPIAYEMDPLVVPEGYVYVMGDNRNNSCDSHNWGPLAIENIVGRSLFKYWPPPPKGSSM 360

Query: 361 VDELRVGKINLGIS 369
            D     KI LGIS
Sbjct: 361 ADSPHASKIKLGIS 373

BLAST of Chy3G064820 vs. TAIR 10
Match: AT1G06870.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 345.1 bits (884), Expect = 6.9e-95
Identity = 195/374 (52.14%), Postives = 251/374 (67.11%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLR--AGNCRVFQEFWVRSCIFGSTHNPEL--KSSGSAR 60
           MA RVT +YS +V +++ASS G R   G+ R   E WVR    G    P++  KS GS  
Sbjct: 1   MAIRVTFTYSSYVARSIASSAGTRVGTGDVRSCFETWVRPRFCGHNQIPDIVDKSPGSNT 60

Query: 61  NYRSESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTS 120
              S   R +P      A++MYST+A E + E  K+P++LG++S++   G          
Sbjct: 61  WGPSSGPRARP------ASSMYSTIAREILEEGCKSPLVLGMISLMNLTGAPQFSGMTGL 120

Query: 121 GVSSFKATSTIPFLQGSKWLPGYDVRSVSDD---VDKGGTT----VCYDYYDKSGNNQFY 180
           G+S FK +S IPFL+GSKW+P     ++S D   VD+GG      V  +  DK  N    
Sbjct: 121 GISPFKTSSVIPFLRGSKWMPCSIPATLSTDIAEVDRGGKVCDPKVKLELSDKVSNG--- 180

Query: 181 ENDFEKSSWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRIL 240
                 + WV++LL+  SEDAKA FTA+TVS+LF+S LAEPKSIPS+SM PTL+VGDR++
Sbjct: 181 -----GNGWVNKLLNICSEDAKAAFTAVTVSLLFRSALAEPKSIPSTSMLPTLDVGDRVI 240

Query: 241 AEKVSYLFRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGV 300
           AEKVSY FRKPEVSDIVIFKAP IL + G S  +VFIKR+VA+ GD VEV  GKL+VN  
Sbjct: 241 AEKVSYFFRKPEVSDIVIFKAPPILVEHGYSCADVFIKRIVASEGDWVEVCDGKLLVNDT 300

Query: 301 AQDEDFVLEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPP 360
            Q EDFVLEPI Y+MEP+ VPEGYV+V+GDNRN S DSHNWGPLPI+NI+GRS+F+YWPP
Sbjct: 301 VQAEDFVLEPIDYEMEPMFVPEGYVFVLGDNRNKSFDSHNWGPLPIKNIIGRSVFRYWPP 360

Query: 361 SKGSAMVDELRVGK 364
           SK S ++   +V +
Sbjct: 361 SKVSDIIHHEQVSQ 360

BLAST of Chy3G064820 vs. TAIR 10
Match: AT2G30440.1 (thylakoid processing peptide )

HSP 1 Score: 344.7 bits (883), Expect = 9.0e-95
Identity = 196/356 (55.06%), Postives = 243/356 (68.26%), Query Frame = 0

Query: 1   MAFRVTLSYSGHVVQNLASSTGLRAGNCRVFQEFWVRSCIFGSTHNPELKSSGSARNYRS 60
           MA R+T +YS HV +NL    G R G      E  VR   F  +H  +     S RN   
Sbjct: 1   MAIRITFTYSTHVARNL---VGTRVGPGGYCFESLVRPRFF--SHKRDF--DRSPRN--- 60

Query: 61  ESRRFKPGGSVEKATAMYSTLAGERVGESPKNPMILGLMSMLKSMGDSSVISTGTSGVSS 120
                       +  +MY ++A E +GE  ++P+++GL+S+LKS       +    GVSS
Sbjct: 61  ------------RPASMYGSIARELIGEGSQSPLVMGLISILKSTTGHESSTMNVLGVSS 120

Query: 121 FKATSTIPFLQGSKWLPGYDVRSVSDDVDKGGTTVCYDYYDKSGNNQFYENDFEKSSWVS 180
           FKA+S IPFLQGSKW+       V DDVDKGG TVC D  DK   N         S WV+
Sbjct: 121 FKASSIIPFLQGSKWIKN---PPVIDDVDKGG-TVCDDDDDKESRN-------GGSGWVN 180

Query: 181 RLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKVSYLFRKP 240
           +LLS  SEDAKA FTA+TVS+LF+S LAEPKSIPS+SM PTL+ GDR++AEKVSY FRKP
Sbjct: 181 KLLSVCSEDAKAAFTAVTVSILFRSALAEPKSIPSTSMYPTLDKGDRVMAEKVSYFFRKP 240

Query: 241 EVSDIVIFKAPQIL---QDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVL 300
           EVSDIVIFKAP IL    ++G SS++VFIKR+VA+ GD VEV+ GKL VN + Q+EDFVL
Sbjct: 241 EVSDIVIFKAPPILLEYPEYGYSSNDVFIKRIVASEGDWVEVRDGKLFVNDIVQEEDFVL 300

Query: 301 EPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGS 354
           EP++Y+MEP+ VP+GYV+V+GDNRN S DSHNWGPLPIENIVGRS+F+YWPPSK S
Sbjct: 301 EPMSYEMEPMFVPKGYVFVLGDNRNKSFDSHNWGPLPIENIVGRSVFRYWPPSKVS 323

BLAST of Chy3G064820 vs. TAIR 10
Match: AT3G24590.1 (plastidic type i signal peptidase 1 )

HSP 1 Score: 227.3 bits (578), Expect = 2.1e-59
Identity = 108/185 (58.38%), Postives = 142/185 (76.76%), Query Frame = 0

Query: 174 EKSSWVSRLLSTYSEDAKALFTALTVSVLFKSFLAEPKSIPSSSMCPTLEVGDRILAEKV 233
           EK+      L   S+DA+ +F A+ VS+ F+ F+AEP+ IPS SM PT +VGDR++AEKV
Sbjct: 99  EKNRLFPEWLDFTSDDAQTVFVAIAVSLAFRYFIAEPRYIPSLSMYPTFDVGDRLVAEKV 158

Query: 234 SYLFRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDE 293
           SY FRKP  +DIVIFK+P +LQ+ G +  +VFIKR+VA  GD+VEV  GKL+VNGVA++E
Sbjct: 159 SYYFRKPCANDIVIFKSPPVLQEVGYTDADVFIKRIVAKEGDLVEVHNGKLMVNGVARNE 218

Query: 294 DFVLEPIAYDMEPLLVPEGYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWPPSKGS 353
            F+LEP  Y+M P+ VPE  V+VMGDNRNNS DSH WGPLP++NI+GRS+F+YWPP++ S
Sbjct: 219 KFILEPPGYEMTPIRVPENSVFVMGDNRNNSYDSHVWGPLPLKNIIGRSVFRYWPPNRVS 278

Query: 354 AMVDE 359
             V E
Sbjct: 279 GTVLE 283

BLAST of Chy3G064820 vs. TAIR 10
Match: AT1G23465.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 76.6 bits (187), Expect = 4.5e-14
Identity = 46/131 (35.11%), Postives = 74/131 (56.49%), Query Frame = 0

Query: 217 SMCPTLE-VGDRILAEKVSYLFRKPEVSDIVIFKAPQILQDFGVSSDEVFIKRVVATSGD 276
           SM PTL   G+ +LAE++S  ++KP   DIV+ ++P+       + ++  IKRVV   GD
Sbjct: 47  SMIPTLHPSGNMLLAERISKRYQKPSRGDIVVIRSPE-------NPNKTPIKRVVGVEGD 106

Query: 277 VVEVQKGKLVVNGVAQDEDFVLEPIAYD-MEPLLVPEGYVYVMGDNRNNSCDSHNWGPLP 336
            +                 FV++P+  D  + ++VP+G+V+V GD  +NS DS N+GP+P
Sbjct: 107 CI----------------SFVIDPVKSDESQTIVVPKGHVFVQGDYTHNSRDSRNFGPVP 154

Query: 337 IENIVGRSLFK 346
              I GR L++
Sbjct: 167 YGLIQGRVLWR 154

BLAST of Chy3G064820 vs. TAIR 10
Match: AT1G29960.1 (Peptidase S24/S26A/S26B/S26C family protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.9e-13
Identity = 50/157 (31.85%), Postives = 82/157 (52.23%), Query Frame = 0

Query: 193 LFTALTVSVLFKSFLAEPKSIPSSSMCPTLE-VGDRILAEKVSYLFRKPEVSDIVIFKAP 252
           L+  L V+  +  F+A        SM PTL   G+ +LAE++S  ++KP   DIV+ ++P
Sbjct: 26  LYCFLHVTTNYLGFMAYAY---GPSMTPTLHPSGNVLLAERISKRYQKPSRGDIVVIRSP 85

Query: 253 QILQDFGVSSDEVFIKRVVATSGDVVEVQKGKLVVNGVAQDEDFVLEPIAYDMEPLLVPE 312
           +       + ++  IKRV+   GD +       V++    DE           + ++VP+
Sbjct: 86  E-------NPNKTPIKRVIGIEGDCI-----SFVIDSRKSDES----------QTIVVPK 145

Query: 313 GYVYVMGDNRNNSCDSHNWGPLPIENIVGRSLFKYWP 349
           G+V+V GD  +NS DS N+G +P   I GR L++ WP
Sbjct: 146 GHVFVQGDYTHNSRDSRNFGTVPYGLIQGRVLWRVWP 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M9Z29.7e-9452.14Probable thylakoidal processing peptidase 2, chloroplastic OS=Arabidopsis thalia... [more]
O043481.3e-9355.06Thylakoidal processing peptidase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q8H0W12.9e-5858.38Chloroplast processing peptidase OS=Arabidopsis thaliana OX=3702 GN=PLSP1 PE=2 S... [more]
P726603.6e-4048.78Probable signal peptidase I-1 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX... [more]
P731573.9e-3440.00Probable signal peptidase I-2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX... [more]
Match NameE-valueIdentityDescription
A0A0A0LNI71.7e-20297.83Peptidase_S26 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G3607... [more]
A0A1S3BBL01.2e-19594.31thylakoidal processing peptidase 1, chloroplastic-like OS=Cucumis melo OX=3656 G... [more]
A0A5A7VHF77.7e-19594.04Thylakoidal processing peptidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1CT201.1e-16983.16thylakoidal processing peptidase 1, chloroplastic-like OS=Momordica charantia OX... [more]
A0A6J1GIQ51.4e-16480.70thylakoidal processing peptidase 1, chloroplastic-like isoform X1 OS=Cucurbita m... [more]
Match NameE-valueIdentityDescription
XP_004152720.17.15e-25997.83thylakoidal processing peptidase 1, chloroplastic [Cucumis sativus] >KGN62554.1 ... [more]
XP_008444657.15.13e-25094.31PREDICTED: thylakoidal processing peptidase 1, chloroplastic-like [Cucumis melo][more]
KAA0065275.15.98e-24994.04thylakoidal processing peptidase 1 [Cucumis melo var. makuwa][more]
XP_038884798.19.27e-23789.73thylakoidal processing peptidase 1, chloroplastic-like [Benincasa hispida][more]
XP_022144217.11.13e-21583.16thylakoidal processing peptidase 1, chloroplastic-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G06870.16.9e-9552.14Peptidase S24/S26A/S26B/S26C family protein [more]
AT2G30440.19.0e-9555.06thylakoid processing peptide [more]
AT3G24590.12.1e-5958.38plastidic type i signal peptidase 1 [more]
AT1G23465.14.5e-1435.11Peptidase S24/S26A/S26B/S26C family protein [more]
AT1G29960.12.9e-1331.85Peptidase S24/S26A/S26B/S26C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000223Peptidase S26A, signal peptidase IPRINTSPR00727LEADERPTASEcoord: 206..222
score: 51.54
coord: 309..328
score: 57.74
coord: 265..277
score: 47.07
IPR000223Peptidase S26A, signal peptidase ITIGRFAMTIGR02227TIGR02227coord: 191..348
e-value: 6.5E-39
score: 131.4
IPR000223Peptidase S26A, signal peptidase IPANTHERPTHR43390SIGNAL PEPTIDASE Icoord: 1..362
IPR019533Peptidase S26PFAMPF10502Peptidase_S26coord: 190..347
e-value: 4.0E-41
score: 140.8
IPR019533Peptidase S26CDDcd06530S26_SPase_Icoord: 209..341
e-value: 9.21993E-25
score: 94.5747
NoneNo IPR availableGENE3D2.10.109.10Umud Fragment, subunit Acoord: 203..364
e-value: 4.0E-36
score: 125.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..67
NoneNo IPR availablePANTHERPTHR43390:SF2THYLAKOIDAL PROCESSING PEPTIDASE 2, CHLOROPLASTIC-RELATEDcoord: 1..362
IPR019756Peptidase S26A, signal peptidase I, serine active sitePROSITEPS00501SPASE_I_1coord: 215..222
IPR019758Peptidase S26A, signal peptidase I, conserved sitePROSITEPS00761SPASE_I_3coord: 314..327
IPR036286LexA/Signal peptidase-like superfamilySUPERFAMILY51306LexA/Signal peptidasecoord: 205..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G064820.1Chy3G064820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006465 signal peptide processing
biological_process GO:0010027 thylakoid membrane organization
biological_process GO:0006508 proteolysis
cellular_component GO:0009535 chloroplast thylakoid membrane
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity