Chy3G063130 (gene) Cucumber (hystrix) v1

Overview
NameChy3G063130
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionFSH1 domain-containing protein
LocationchrH03: 16307783 .. 16310955 (-)
RNA-Seq ExpressionChy3G063130
SyntenyChy3G063130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGTGAGAGCAAAACTGAAAGGAAACCCAAGATTTTGTGCCTCCACGGCTTCCGAACTAGTGGAGCAATCTTGAGGAAGCAGGACCAAAGATGGCCAACTTCTGTTCTTCATCAATTCCATCTCCATTTTATCGACGGCCCATTCCCTTCCAAAGGAAAATCAGACGTCGAAGGAATTTACGATCCTCCCTATTTCGAGTGGTTTGGAACCAGCGAGGTCCATATTAATTGCTTTTTCTGTTTCTTTTTTTTTCTTTCAATTCTCTCTATATTTCAAATACTGATTCATTCAATTTCTCAGGACCCGACCAGTTGTGAAAATTTGGAATCGAGCCTTGAATTCATCGAGAGTTACATGGCTGAGCACGGACCATTCGACGGACTTCTCGGTTTTTCACAGGTAATTAAGGGTTGATTCTCTTGAATCCGATTTGGATGGATTTAATTAATAATTTCATAATTTGAAGCTATTTTCAGGGGGCGGTGTTATCGGCAGCACTGGCGTTGCTTCAAGCGAGGGTAACTAAACACCGATGGCCGGTTGTTCTTCGTTTTAATCTCTGTTTTCAATTTTCGGAAATTGAATTTTAATCGGCTTGCAGGGAGTTGCACTGACGAAGGTCCCGAAGATCAAATTCGTAATAATAATTAGCGGTTCGAAACTGCAATCATCATCCTTGGCCGCCCGAATAGCTTATTCTACGTCTATTGCATGCCCATCCCTCCATTTTCTAAGTAACTTTCCGGCGTCTTCATGTTTCTCGTTTTATATTTTTTCTATTTAAAGTTCAAATACATCTATTAGAAAAAATATAATTTTACCTAAATATAAAAGGCGGTTTTGATTCACTTAAAAGAAGAAAATGTTAATGAAAAATTATTTTTTTCTTAAAAGAAAATTATTTAAAATATACTCTAAAAACTATTCTCAATCCTAACCACACAATAAAAAAGGACAAAAACTTCAAAATTTAGAGATTAAACTTTATAATTTAACACTAATTTATTTAAATATATTGAAATACTTCTGAGATGACAACATATACCTTCATCAACACTCAATATTATCATTATAGCAAATTTGACAAAATATTTACAATCTATAGCAAATTTAATCACTGATAGTAACTGGTATGCTATAGTAATAGAAAACTATCATTAATATAATTTAAAATTTTGAATATTTTAATTTATTTTTTATTTTTAAATATGCAAAGGGTTTTTTAAAAATATATATATATAAAAAAAAACAAACAATTTACTAGAAAAAATGTTATTTCGTTATTTTTTTTTATGAAAGGTAAATATGTTGTAATTTTTTAAGATAAATTTCCATATTGCAATGTGAGTCTGACTTTCTTTGTTTCAATATTTGGCATCGAACATCGAATCAATAAATGCCTTTGTAAACGATGAAAAGCAAAAAATCAAATAATCAAATGCTCTGATGATTGATTGAAAATCAAATCAATTCATTTTCTAATTTTACACTTTTTATTGCATTTCACATTATTAAAATTGATTTTTAAACTACTAAGATAATTTTCAAGTAATTTATAATTAAAATGACAAAAACAATTTAAACCATTTTAAAATAACAGTGCTTTGCATGTCACTGTGATTTGTATACGTAGGTGAGGAAGACTTCATGATGCCCAGTGGGTTGAAGCTTCTACAATCATTTGTTGAACCATTAGTAATTAATCATTCTAAAGGCCATATAGTACCAAGACTTGGTAATATTTAATTTATTCACCTATTTATATATATACATATATGATTTTTTATGGTAATTTAGATATTTATATAATTTTATTCTGTAGATGAAAAAAGTTTGAAGATCGTGGACGGATTTATCCAAAAGATTTCAAAGCTATGACCACCTCAAACTAAAGAACACACGTAGGCTTTTAAATAATTGAATTGAATTTACTTCTTAAATAAGCTTTCGAACTTTTTATCGAGAGTACATATCTTGATTAAGTACGAGGATTTGAATTGTAGAATCCTAATTTTGTTGGGATTTTTTATTTATTGTTTTTGTGTTATGGATTTTATTATTATATCGATACAATCCAAATATTAAAATGTTAGAGTCTAAGTATCGTTATTATTTGATAAACTTCACTACTTAGTTGATACTCAATCTGTTAAAGTCTAAGTATAAATGTTAATTTAAAAAAAAAAGTTCTATAAATCGGATTAATCCAAAACTAGTAGTATTAATAATGATAAAAGTAACAAAGATTTTTCTCCTGAGTATAAATGTTTGCTTTAAATACCTACAAATTTAATTAACTTAAACCACCTTTATTACAATTTTCTAAAGAAATAAATACACAATCCCCATAGCAACTTCATTGCTATGAAATTAACATTACTTATTAAAATCTTAAATTAATAATTTTGTATCCATCTATTCAAATCTTGATTTTTTAAATTATTATTTGAATTGAATAAGACCATTTATTAGTTATTCATAAATATTTTTCACTCCATTCCATTTACAACTTAACTCAAAATTACTTAAATTCCTTTCAAATTCTACCTAAGAAAATTATGTAATGCATAATTCTACCTAATTAAATGAAATTGAAAAACACTACTCATACGAATAAAAGTATCCAAATGACTCTAATGTTTGTCAGGAACATTAAATTAATCTTACCTTTTAAAAGAAATCTAATTTCCTAAATGTTACAAGTATGTATTAATAATTTTGAAACAAGCGTAAATCAAATTAATTTGTTTGAGAGATCCAGAGTTTGTAAAAGGGTAATTGTTAGGAATAGAAAAATATATATATATTTACAAAATATAGAAAAAAAATATATGGAAGGTTTGATGAATTTTGGATCCATTTTGAACAGTTTCAATATTTTGTAATATATGGAATTGAATGGTATATTAAAATTATATGGCTAATATTTTCATTTTTATTGCTTTTAGTTTATCAATTATGAATTTGATTTAAATTTAGTTTTTAAGTTTTCAAAATGTTCAAATTTGTACTTAAGATTTCAACCTTGTTTCAATTTAGTTTATAGAATTCAAGAAATACATTTATGACAAATTACTCACTTTTAATCTTTGGTATTTAATTTATGTTGATGCTTGAATCTAGTCTACCTTGAGTTGCGTCAGGTGTCGAATGGTCTCGTTCTCTAA

mRNA sequence

ATGGCAAGTGAGAGCAAAACTGAAAGGAAACCCAAGATTTTGTGCCTCCACGGCTTCCGAACTAGTGGAGCAATCTTGAGGAAGCAGGACCAAAGATGGCCAACTTCTGTTCTTCATCAATTCCATCTCCATTTTATCGACGGCCCATTCCCTTCCAAAGGAAAATCAGACGTCGAAGGAATTTACGATCCTCCCTATTTCGAGTGGTTTGGAACCAGCGAGGACCCGACCAGTTGTGAAAATTTGGAATCGAGCCTTGAATTCATCGAGAGTTACATGGCTGAGCACGGACCATTCGACGGACTTCTCGGTTTTTCACAGGGGGCGGTGTTATCGGCAGCACTGGCGTTGCTTCAAGCGAGGGGAGTTGCACTGACGAAGGTCCCGAAGATCAAATTCGTAATAATAATTAGCGGTTCGAAACTGCAATCATCATCCTTGGCCGCCCGAATAGCTTATTCTACGTCTATTGCATGCCCATCCCTCCATTTTCTAATCTACCTTGAGTTGCGTCAGGTGTCGAATGGTCTCGTTCTCTAA

Coding sequence (CDS)

ATGGCAAGTGAGAGCAAAACTGAAAGGAAACCCAAGATTTTGTGCCTCCACGGCTTCCGAACTAGTGGAGCAATCTTGAGGAAGCAGGACCAAAGATGGCCAACTTCTGTTCTTCATCAATTCCATCTCCATTTTATCGACGGCCCATTCCCTTCCAAAGGAAAATCAGACGTCGAAGGAATTTACGATCCTCCCTATTTCGAGTGGTTTGGAACCAGCGAGGACCCGACCAGTTGTGAAAATTTGGAATCGAGCCTTGAATTCATCGAGAGTTACATGGCTGAGCACGGACCATTCGACGGACTTCTCGGTTTTTCACAGGGGGCGGTGTTATCGGCAGCACTGGCGTTGCTTCAAGCGAGGGGAGTTGCACTGACGAAGGTCCCGAAGATCAAATTCGTAATAATAATTAGCGGTTCGAAACTGCAATCATCATCCTTGGCCGCCCGAATAGCTTATTCTACGTCTATTGCATGCCCATCCCTCCATTTTCTAATCTACCTTGAGTTGCGTCAGGTGTCGAATGGTCTCGTTCTCTAA

Protein sequence

MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGIYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQARGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFLIYLELRQVSNGLVL*
Homology
BLAST of Chy3G063130 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 4.1e-13
Identity = 52/167 (31.14%), Postives = 76/167 (45.51%), Query Frame = 0

Query: 12  KILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSD------------VE 71
           K+LCLHG+  SG +  K+       +     LHF  GP  +  ++D            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 72  GIYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQ 131
           G  +   F WF   +   +  + + SLE I  YM E GPFDGL+GFSQGA + A LA + 
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 132 ARGV---ALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLH 164
             G       + P  KFV+ + G + +         Y+  +  PSLH
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFDH--FYNPKLTTPSLH 170

BLAST of Chy3G063130 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.0e-11
Identity = 54/169 (31.95%), Postives = 79/169 (46.75%), Query Frame = 0

Query: 12  KILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGI---------Y 71
           +ILCL GFR S    R++      ++  +  L  + GP P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 72  DPPYFEWFGTS--------EDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAA 131
           + P   WF           E+PT+C  LE +LE +   + + GPFDG+LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 132 LALLQARGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLH 164
           +  L   G    + P  +FVI++SG   +   L   I     ++ PSLH
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQG-PLSLPSLH 173

BLAST of Chy3G063130 vs. ExPASy Swiss-Prot
Match: S0EES1 (Esterase FUS5 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) OX=1279085 GN=FUS5 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.9e-11
Identity = 42/126 (33.33%), Postives = 67/126 (53.17%), Query Frame = 0

Query: 10  KPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGIYDPPYFEW 69
           +P++LCLHG   S  I+R Q  +  +++   F L F++GP  S     V      P+FE 
Sbjct: 4   RPRLLCLHGGGASSQIMRVQFSKLESALRKTFQLVFLEGPLDSAPGPGV-----LPFFED 63

Query: 70  FGTSEDPTSC--------------ENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAAL 122
           FG    P SC              +   +++ +I+++M ++GPF G+LGFSQGA  +A++
Sbjct: 64  FG----PYSCWVSDDKSLLPEEKRKEENNAIAYIKTFMLQYGPFAGILGFSQGARATASI 120

BLAST of Chy3G063130 vs. ExPASy Swiss-Prot
Match: W7MWX7 (Esterase FUS5 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN=FUS5 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.5e-11
Identity = 38/117 (32.48%), Postives = 64/117 (54.70%), Query Frame = 0

Query: 10  KPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGIYDP--PYF 69
           +P++LCLHG   S  I+R Q  +  +++   F L F++GP  S     V   +    PY 
Sbjct: 4   RPRLLCLHGGGASSQIMRIQFSKLESALRKTFQLVFLEGPLDSAPGPGVLPFFKDFGPYS 63

Query: 70  EWFGTSEDPTSCENLE---SSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQAR 122
            W       +  E  +   +++ +I+++M ++GPF G+LGFSQGA  +A++ L Q R
Sbjct: 64  CWVSDDRSLSPEEKRQEETNAIAYIKTFMLQYGPFAGILGFSQGARAAASILLEQQR 120

BLAST of Chy3G063130 vs. ExPASy Swiss-Prot
Match: Q8WZ82 (Esterase OVCA2 OS=Homo sapiens OX=9606 GN=OVCA2 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.2e-10
Identity = 51/173 (29.48%), Postives = 75/173 (43.35%), Query Frame = 0

Query: 8   ERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGI------ 67
           +R  ++LCL GFR S    R++      ++  +  L  + GP P       EG       
Sbjct: 4   QRPLRVLCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVPDPPGPEGARSDFGS 63

Query: 68  ---YDPPYFEWFGTS--------EDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAV 127
               + P   WF           E+P  C  LE SL  +   +   GPFDGLLGFSQGA 
Sbjct: 64  CPPEEQPRGWWFSEQEADVFSALEEPAVCRGLEESLGMVAQALNRLGPFDGLLGFSQGAA 123

Query: 128 LSAALALLQARGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLH 164
           L+A +  L   G    + P  +F++++SG   +       I     ++ PSLH
Sbjct: 124 LAALVCALGQAGD--PRFPLPRFILLVSGFCPRGIGFKESI-LQRPLSLPSLH 173

BLAST of Chy3G063130 vs. ExPASy TrEMBL
Match: A0A0A0LLR4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369790 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.1e-85
Identity = 159/165 (96.36%), Postives = 162/165 (98.18%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           MASE K ERKPKILCLHGFRTSGAILRKQ QRWPTS+LHQFHLHFIDGPFPSKG+SDVEG
Sbjct: 1   MASERKIERKPKILCLHGFRTSGAILRKQVQRWPTSILHQFHLHFIDGPFPSKGRSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           RGVALTKVPKIKFVI+ISGSKLQSSSLAARIAYSTSIACPSLHFL
Sbjct: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFL 165

BLAST of Chy3G063130 vs. ExPASy TrEMBL
Match: A0A5A7VDJ4 (Esterase OVCA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004520 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.2e-78
Identity = 149/165 (90.30%), Postives = 154/165 (93.33%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           M SESK ERKP ILCLHGFRTSGAILRKQ Q+WPTSVLHQFH HFIDGP PS+GKSDVEG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           IYDPPYFEWFGTS+DPTSCENLES LEFIE+YM +HGPFDGLLGFSQGAVLSAALA LQA
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQGAVLSAALAGLQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           RGVALTKVPKIKFVIIISGSKLQSSSLAA IAYS SIACPSLHFL
Sbjct: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAAGIAYSPSIACPSLHFL 165

BLAST of Chy3G063130 vs. ExPASy TrEMBL
Match: A0A1S3BC58 (esterase OVCA2-like OS=Cucumis melo OX=3656 GN=LOC103488096 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.2e-78
Identity = 149/165 (90.30%), Postives = 154/165 (93.33%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           M SESK ERKP ILCLHGFRTSGAILRKQ Q+WPTSVLHQFH HFIDGP PS+GKSDVEG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           IYDPPYFEWFGTS+DPTSCENLES LEFIE+YM +HGPFDGLLGFSQGAVLSAALA LQA
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQGAVLSAALAGLQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           RGVALTKVPKIKFVIIISGSKLQSSSLAA IAYS SIACPSLHFL
Sbjct: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAAGIAYSPSIACPSLHFL 165

BLAST of Chy3G063130 vs. ExPASy TrEMBL
Match: A0A6J1K941 (dihydrofolate reductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492222 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 5.1e-59
Identity = 112/165 (67.88%), Postives = 138/165 (83.64%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           M S+S+ +RKP+ILCLHGFRTSGAIL+K  ++WPTSVL+QF  HF+D PFPS+GKS+VEG
Sbjct: 1   MGSKSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           ++DPPY+EWF   ED     N+E  L+FIE++M EHGPFDGLLGFSQGA L+A L   QA
Sbjct: 61  LHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           +GVALT+VPKIKFVIII+G KL+S SL A+ AY++SIACPSLHFL
Sbjct: 121 KGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFL 165

BLAST of Chy3G063130 vs. ExPASy TrEMBL
Match: A0A6J1HDC8 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462485 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.1e-58
Identity = 114/165 (69.09%), Postives = 135/165 (81.82%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           M SES  +RKP+ILCLHGFRTSGAIL+K  ++WPTSVL QF  HF+D PFPS+GKS+VEG
Sbjct: 1   MGSESNIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLDQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           ++DPPYFEWF   ED     N+E  L+FIE++M EHGPFDGLLGFSQGA L+A L   QA
Sbjct: 61  LHDPPYFEWFQFHEDTADFANVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           +GVALT+VPKIKFVIIISG KL+S SL A+ A ++SIACPSLHFL
Sbjct: 121 KGVALTRVPKIKFVIIISGVKLESPSLDAQEASASSIACPSLHFL 165

BLAST of Chy3G063130 vs. NCBI nr
Match: XP_004152616.2 (esterase OVCA2 [Cucumis sativus])

HSP 1 Score: 325 bits (834), Expect = 3.90e-111
Identity = 159/165 (96.36%), Postives = 162/165 (98.18%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           MASE K ERKPKILCLHGFRTSGAILRKQ QRWPTS+LHQFHLHFIDGPFPSKG+SDVEG
Sbjct: 1   MASERKIERKPKILCLHGFRTSGAILRKQVQRWPTSILHQFHLHFIDGPFPSKGRSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 165
           RGVALTKVPKIKFVI+ISGSKLQSSSLAARIAYSTSIACPSLHFL
Sbjct: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFL 165

BLAST of Chy3G063130 vs. NCBI nr
Match: KGN62961.2 (hypothetical protein Csa_022631 [Cucumis sativus])

HSP 1 Score: 323 bits (828), Expect = 3.20e-110
Identity = 160/165 (96.97%), Postives = 161/165 (97.58%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           MASESKTERKPKILCLHGFRTSGAILRKQ QRWPTSVLHQFHLHFID   PSKGKSDVEG
Sbjct: 1   MASESKTERKPKILCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 165
           RGVALTKVPKIKFVI+ISGSKLQSSSLAARIAYSTSIACPSLHFL
Sbjct: 121 RGVALTKVPKIKFVIVISGSKLQSSSLAARIAYSTSIACPSLHFL 165

BLAST of Chy3G063130 vs. NCBI nr
Match: XP_004152615.3 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 310 bits (794), Expect = 4.84e-105
Identity = 153/165 (92.73%), Postives = 157/165 (95.15%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           MASESKTERKPKILCLHGFRTSGAILRKQ QRWPTSVLHQFHLHFID   PSKGKSDVEG
Sbjct: 1   MASESKTERKPKILCLHGFRTSGAILRKQVQRWPTSVLHQFHLHFIDDSIPSKGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           IYDPPYFEWFGTSEDPT+ ENLESS+EFIESYM EHGPFDGLLGFSQGA+LSAALALLQA
Sbjct: 61  IYDPPYFEWFGTSEDPTNYENLESSIEFIESYMLEHGPFDGLLGFSQGAMLSAALALLQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 165
           RGVALTKVPKIKFVI+IS SKLQSSSLAARI YSTSIACPSLHFL
Sbjct: 121 RGVALTKVPKIKFVIVISSSKLQSSSLAARIVYSTSIACPSLHFL 165

BLAST of Chy3G063130 vs. NCBI nr
Match: XP_008444881.1 (PREDICTED: esterase OVCA2-like [Cucumis melo] >KAA0065117.1 esterase OVCA2-like [Cucumis melo var. makuwa])

HSP 1 Score: 303 bits (776), Expect = 2.67e-102
Identity = 149/165 (90.30%), Postives = 154/165 (93.33%), Query Frame = 0

Query: 1   MASESKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEG 60
           M SESK ERKP ILCLHGFRTSGAILRKQ Q+WPTSVLHQFH HFIDGP PS+GKSDVEG
Sbjct: 1   MTSESKIERKPTILCLHGFRTSGAILRKQIQKWPTSVLHQFHFHFIDGPLPSQGKSDVEG 60

Query: 61  IYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQA 120
           IYDPPYFEWFGTS+DPTSCENLES LEFIE+YM +HGPFDGLLGFSQGAVLSAALA LQA
Sbjct: 61  IYDPPYFEWFGTSKDPTSCENLESCLEFIENYMVKHGPFDGLLGFSQGAVLSAALAGLQA 120

Query: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 165
           RGVALTKVPKIKFVIIISGSKLQSSSLAA IAYS SIACPSLHFL
Sbjct: 121 RGVALTKVPKIKFVIIISGSKLQSSSLAAGIAYSPSIACPSLHFL 165

BLAST of Chy3G063130 vs. NCBI nr
Match: XP_038884223.1 (dihydrofolate reductase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 259 bits (662), Expect = 2.68e-85
Identity = 128/172 (74.42%), Postives = 140/172 (81.40%), Query Frame = 0

Query: 5   SKTERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGIYDP 64
           SK +RKPKILCLHGFRTSGAIL+KQ ++WP  VLHQFH HFID PFPSKGKSDV+GIYDP
Sbjct: 2   SKIQRKPKILCLHGFRTSGAILKKQIEKWPAFVLHQFHFHFIDAPFPSKGKSDVQGIYDP 61

Query: 65  PYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQARGVA 124
           PYFEWF  S+D T  EN ES LEFIE++M  HGPFDGLLGFSQGA L+AAL  LQA+GVA
Sbjct: 62  PYFEWFQFSKDTTGYENFESCLEFIENHMLNHGPFDGLLGFSQGAALTAALPGLQAKGVA 121

Query: 125 LTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFLIYLELRQVSNG 176
           L KVPKIKFVIIISGSKL S SLAA  AY  SIACPSLHFL    L  +S+ 
Sbjct: 122 LRKVPKIKFVIIISGSKLGSPSLAAEKAYFPSIACPSLHFLSNFHLLLISSN 173

BLAST of Chy3G063130 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 191.4 bits (485), Expect = 6.2e-49
Identity = 96/166 (57.83%), Postives = 123/166 (74.10%), Query Frame = 0

Query: 1   MASESKT-ERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVE 60
           M SE ++  RKP+ LCLHGFRTSG I++ Q  +WP SV+ +  L F+D PFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQ 120
           GI+DPPY+EWF  +++ T   N E  LE++E  M + GPFDGL+GFSQGA+LS  L  LQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 ARGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           A+G+A  KVPKIKFVIII G+KL+S+ LA   AYS+S+   SLHFL
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAEN-AYSSSLETLSLHFL 165

BLAST of Chy3G063130 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 191.4 bits (485), Expect = 6.2e-49
Identity = 96/166 (57.83%), Postives = 123/166 (74.10%), Query Frame = 0

Query: 1   MASESKT-ERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVE 60
           M SE ++  RKP+ LCLHGFRTSG I++ Q  +WP SV+ +  L F+D PFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIYDPPYFEWFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQ 120
           GI+DPPY+EWF  +++ T   N E  LE++E  M + GPFDGL+GFSQGA+LS  L  LQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 ARGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSIACPSLHFL 166
           A+G+A  KVPKIKFVIII G+KL+S+ LA   AYS+S+   SLHFL
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAEN-AYSSSLETLSLHFL 165

BLAST of Chy3G063130 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 2.1e-44
Identity = 84/161 (52.17%), Postives = 113/161 (70.19%), Query Frame = 0

Query: 9   RKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGIYDPPYFE 68
           + P+ILCLHGFRTSG IL+    +WP ++L    L F+D PFP+ GKSDVE  +DPPY+E
Sbjct: 28  KNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYE 87

Query: 69  WFGTSEDPTSCENLESSLEFIESYMAEHGPFDGLLGFSQGAVLSAALALLQARGVALTKV 128
           W+  ++      N E  L +IE YM ++GPFDGLLGFSQGA L+AA+  +Q +G ALTKV
Sbjct: 88  WYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKV 147

Query: 129 PKIKFVIIISGSKLQ----SSSLAARIAYSTSIACPSLHFL 166
           PK+KF++IISG+K+         AA  A+S+ + CPSLHF+
Sbjct: 148 PKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFI 188

BLAST of Chy3G063130 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 64.7 bits (156), Expect = 8.7e-11
Identity = 56/186 (30.11%), Postives = 83/186 (44.62%), Query Frame = 0

Query: 7   TERKPKILCLHGFRTSGAILRKQDQRWPTSVLHQFHLHFIDGPFPSKGKSDVEGIYD--- 66
           T RK +ILCLHGFR + +  + +       + +   L FID P       +++ IY    
Sbjct: 350 TRRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAP------HELQFIYQTAT 409

Query: 67  PP------YFEWFGTSE----------------DP----TSCENLESSLEFIESYMAEHG 126
           PP       F W  +S+                DP    T  E  + SL ++++   E G
Sbjct: 410 PPSGVCNKKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKG 469

Query: 127 PFDGLLGFSQGAVLSAALALLQARGVALTKVPKIKFVIIISGSKLQSSSLAARIAYSTSI 164
           PFDG+LGFSQGA ++AA+   Q +   L      +F ++ SG    +      +    SI
Sbjct: 470 PFDGILGFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSGF---TPWPLLEMKEKRSI 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P365914.1e-1331.14Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) O... [more]
Q3SZ071.0e-1131.95Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1[more]
S0EES12.9e-1133.33Esterase FUS5 OS=Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-68... [more]
W7MWX78.5e-1132.48Esterase FUS5 OS=Gibberella moniliformis (strain M3125 / FGSC 7600) OX=334819 GN... [more]
Q8WZ824.2e-1029.48Esterase OVCA2 OS=Homo sapiens OX=9606 GN=OVCA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LLR44.1e-8596.36FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369790 PE=4 S... [more]
A0A5A7VDJ42.2e-7890.30Esterase OVCA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G0... [more]
A0A1S3BC582.2e-7890.30esterase OVCA2-like OS=Cucumis melo OX=3656 GN=LOC103488096 PE=4 SV=1[more]
A0A6J1K9415.1e-5967.88dihydrofolate reductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492... [more]
A0A6J1HDC81.1e-5869.09dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462485 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004152616.23.90e-11196.36esterase OVCA2 [Cucumis sativus][more]
KGN62961.23.20e-11096.97hypothetical protein Csa_022631 [Cucumis sativus][more]
XP_004152615.34.84e-10592.73dihydrofolate reductase [Cucumis sativus][more]
XP_008444881.12.67e-10290.30PREDICTED: esterase OVCA2-like [Cucumis melo] >KAA0065117.1 esterase OVCA2-like ... [more]
XP_038884223.12.68e-8574.42dihydrofolate reductase-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G24380.16.2e-4957.83INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and deri... [more]
AT4G24380.26.2e-4957.83CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidop... [more]
AT5G65400.12.1e-4452.17alpha/beta-Hydrolases superfamily protein [more]
AT1G09280.18.7e-1130.11CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolas... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005645Serine hydrolase FSHPFAMPF03959FSH1coord: 9..165
e-value: 1.6E-37
score: 129.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 6..165
e-value: 9.7E-40
score: 138.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 7..163
NoneNo IPR availablePANTHERPTHR48070ESTERASE OVCA2coord: 6..165
NoneNo IPR availablePANTHERPTHR48070:SF5DIHYDROFOLATE REDUCTASEcoord: 6..165

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G063130.1Chy3G063130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus