Chy3G062440 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G062440
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Malic enzyme
Location	chrH03: 15693278 .. 15697640 (+)
RNA-Seq Expression	Chy3G062440
Synteny	Chy3G062440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGTTCAATGAAGAAGACAATGAGCAACGGTAGTGTTCATATGGAGATGGAAGAAATTGACAATAAGTTTGAAGAAGCCTATGGAGAACACAATGCTACTGAAGATGAGCTCATTACTCCTTGGACAGTCTCTGTTGCTAGGTAAAGGATTTAAGGAAGATCTTTGCTACTAAAAACACGTTCTGTTTATTTTTTTTTTATTTTTTGTGGTTGTTTTTGCATTTGTTCTAACTTGAAAATTTTCTCATTTTTGTCTGTCTTTTGTTGTTTTAGTGGTTATTCTTTACTGCGTGATCCGATCTACAACAAAGGCCTCGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCAGTTATTGATCAAGAGCTTCAGGTAAAGTTTAGATCTTTCTAGTTCTACCATTTTCTCTTTTATTTACTTTTTTGTTGGAATGTCTTCCTATCTTGCGTTTGGGATGGTCGAGTTAAAATTCAGAGGGGTACACTACCTAAGATGTCTCACGCTAGTGGTGTCGTGATCATGTTATATGATATTTTCTCTCTAATAATAAACTTAAGACACTTATAGCAAACTATGCAAATCTTTTTGTCGACACTACACTCTCTTACTTTACATTTTCTGTTTGTCTTTGTTGATAGCTTAACACTTTTTCTTTTTTTATGGTATTGATTTGGATGCTTGTTTACGCCTTTTTTTTTTTGTGGGAAGCAGGAAAGGAGGATGATGCATAATCTTCGCAACTATGAAGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGTTCGATATATGTTTCACTTGTTTATTGTTAATTTTCCTATTTGATTCCTCTCTCTGCAGCTAATGAGTTAAAGATCATTAGATGAAAAATATGAGTGTCATTGATATAAGGAGCTATGTGGGGTTTGTCTTTGGAATTGAATTTTTAGGCTATATTATTAAAACGTATCTCTAAACTATGCTTTTTGTTTAAAAATATTCTTATTCTTTCAAAAGTCCTAAGATTACTTTGACCTTATATAAATGTTAAAATTATCTTGGAGTAGAAATTCTTTATGATTTCTTATAGAAGTTGGGACTTGGTACTGTGTGGCGATCCAAAATTCACACTTTTTAGTCATATCATTAAACTGCACACAAACAAGACTCTAAATCTTATTATCTATGATATGTAAGATGTTAATTTCAATCAAAGCCTGTTAGACAACCCAATTCTTTCTCCTTATCTCAATTGATGTTTGTAATTGTAGCTTTGTTCTACCTGGCCATACCAAAATTTTTCCTTCATGTCTACTATCATTTGACCTTGCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATTGATAACGTTGAAGAACTGCTTCCAATTGTGTACACTCCAACCGTAGGTGAAGCTTGCCAGAAGTATGGGAGCGTTTATAGACGACCTCAAGGTCTTTTCATCAGTTTGAAAGAGAAGTATGTCATTAAACTAGCATTTATAATGTATGATTAGTTGCATTAGTTCTTAACTGATAATCAACAATTTACCTTTCCAGGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGAAACATTCAAGTTATTGTTGTTACCGACGGTGAGCGGATTCTAGGCCTTGGGGATCTTGGATGCCAGGTAATAAAACTTACTGCTTCCATTCAGCTGATGAACCTTCATTTTTTCTTCTATCCTTCAGTTTTTTGTATGCATATTCTCATTTTGTTAAACTGATATCTTCAGGGCATGGGGATTCCTGTGGGGAAACTTTCTTTATATACAGCACTTGGAGGAATTCGACCCTCAGCTGTAAGCAATGCTTTAGTTTATATACTTTAGTTAATCTCTTCCTCCCTTTGTCTATCATCAGTAATCTGAATACAGCAAAGTGTTGATACCTGGAATAGTCAGGCGAGGACTAAAGTTTATAGTTGTTTATTGTGTTCAGTGCTTGCCTATAACCATTGATGTTGGTACAAACAACGAGCAGCTTCTAAATGATGAGTTTTACATTGGCCTTCGGCAGAAACGGGCCAGAGGACAGGTGAAACAATCTTTTACTCTCTTTTTTCCTCATGACTTCATGATTTCAATCCATTCATGCATGACTGTTTTAATCAATTTTTATTTTTTCCTTCATTCAGGAATACATGGAACTTTTAGACGAGTTCATGTATGCAGTAAAGAAGAACTATGGAGAGAAAGTTCTTATACAGGTTATTTGACAATTTTCAACTTACAGTAGGATAGAATTTTGCTTGGAGTTATGTACAAGTGATTCAATGTGTCGCTAACCATTTCTTACTTATTAAACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTCAATGACGACATTCAAGTACGAAATATACACGTGTTTCAGTTCACTCCTAATTTTTATATGTTTCAAGAGTATCTCTAACATGGTTTCAATCATTATGATATTTAGGGTACAGCATCTGTTGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGGACATTAGCTGACCACACTTTCTTATTCCTTGGAGCTGGAGAGGTGAGTTTTAAAATGAGTTATATTTGAAGTGTTTTGAACTGTTTTCGTCAACTTTGGCAGGTATTTCCTTGACGAATCATCTGCTTAAGTTTCATGTCTTGTCCTGTAATTTTTCATTCACTACATTATATGTTTTCTTTGGTATGGTTCTTATTTCTGTCATTTGAGTGTTCTAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGGTATGAACCTATTTCTACTTATCTTCTCTTTTTTATTCTGAATCAATCAAATGCATTTATGGCTAATGAATTTTACTTGTTTTTGGCAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTCGTGGACTCGAAGGTTGTTTGGACTGACTCATTTGAATATTGTGCCTTTACACTCAAATGATCTTTTCTGTTCTAAATGAAATTATTACCATCTATTACAGGGGCTGATCGTTCAATCCCGCTTCGAATCCCTTCAACATTTTAAGAAGCCTTGGGCTCATGACCATGAATCTATCAAGGATCTTTACGGTGCCGTCCAGGTTAGTAATCTACATTGATCAGTCTTGCGTTATGTAGTCATTCTTCATAGTATATGAGACCATTTTTTAACTCAATCTTAAAACTTTAAATTATTAGGCAATCAAACCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAGACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGGTTCTTTCTTGTTCCTTCTAACTTTCTCATTCCTCAGTTCTAAGACAAGGAACTGTTTAAGATTAACTCAAGCGTCGATTACTATGCAGAAACCACTTATCCTTGCTCTCTCGAACCCTACGTCTCAATCGGAGTGTACAGCTGAAGAAGCTTATACATGGAGCCAGGTACAATCCAAGTCTTTCAAAACGGCAGGCATCATTTTTTTTTGTTTTGTTTGTGCTGACCACATCATGACATAAATTCACCAAAAAACGTGCAGGGCCGAGCAATCTTTGCTAGCGGAAGTCCATTTGACCCTGTTGAATACGATGGAAAAGTTTTTGTGCCTGGCCAGGTAGCTTTCATGCTTCAGAAACCATTCTTTTAGTAACCATTCAATTTTAACAGCATGGTTTGATCTTATAAGCTTGCCTTTTCTCTTGGTAATGTCATTTAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGTACAATTCGTGTACATGACGACATGCTCTTGGCAGCCTGTAAGTGAAATTTTGGTTAATTAACCAAACTAATAATGCTTGTTTTGTGCCAATCTTTATTCTCTCTTCCTCCTTCCAATGCTCGTTGAGTTAGTCACTGAGTTGGAGCTCGATTTAACGGTTTTTTTCCCCTCATCGCAGCGGAGGCTCTGGCTGCCCAAGTGAGTCAGGAAAACTATGATAAGGGATTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAACTTGGTTAGCTTCTCTCCCATTTTCTTCACATTGTGTTTTAATTCATTCATAAACAAACCAATGACAGAAAAAGTTAACTAACAACATGGCATTACATTGCAGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTTGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTATCGTAGCTACCTTTAA

mRNA sequence

ATGGAGAGTTCAATGAAGAAGACAATGAGCAACGGTAGTGTTCATATGGAGATGGAAGAAATTGACAATAAGTTTGAAGAAGCCTATGGAGAACACAATGCTACTGAAGATGAGCTCATTACTCCTTGGACAGTCTCTGTTGCTAGTGGTTATTCTTTACTGCGTGATCCGATCTACAACAAAGGCCTCGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCAGTTATTGATCAAGAGCTTCAGGAAAGGAGGATGATGCATAATCTTCGCAACTATGAAGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATTGATAACGTTGAAGAACTGCTTCCAATTGTGTACACTCCAACCGTAGGTGAAGCTTGCCAGAAGTATGGGAGCGTTTATAGACGACCTCAAGGTCTTTTCATCAGTTTGAAAGAGAAGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGAAACATTCAAGTTATTGTTGTTACCGACGGTGAGCGGATTCTAGGCCTTGGGGATCTTGGATGCCAGGGCATGGGGATTCCTGTGGGGAAACTTTCTTTATATACAGCACTTGGAGGAATTCGACCCTCAGCTTGCTTGCCTATAACCATTGATGTTGGTACAAACAACGAGCAGCTTCTAAATGATGAGTTTTACATTGGCCTTCGGCAGAAACGGGCCAGAGGACAGGAATACATGGAACTTTTAGACGAGTTCATGTATGCAGTAAAGAAGAACTATGGAGAGAAAGTTCTTATACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTCAATGACGACATTCAAGGTACAGCATCTGTTGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGGACATTAGCTGACCACACTTTCTTATTCCTTGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTCGTGGACTCGAAGGGGCTGATCGTTCAATCCCGCTTCGAATCCCTTCAACATTTTAAGAAGCCTTGGGCTCATGACCATGAATCTATCAAGGATCTTTACGGTGCCGTCCAGGCAATCAAACCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAGACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGAAACCACTTATCCTTGCTCTCTCGAACCCTACGTCTCAATCGGAGTGTACAGCTGAAGAAGCTTATACATGGAGCCAGGGCCGAGCAATCTTTGCTAGCGGAAGTCCATTTGACCCTGTTGAATACGATGGAAAAGTTTTTGTGCCTGGCCAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGTACAATTCGTGTACATGACGACATGCTCTTGGCAGCCTCGGAGGCTCTGGCTGCCCAAGTGAGTCAGGAAAACTATGATAAGGGATTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAACTTGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTTGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTATCGTAGCTACCTTTAA

Coding sequence (CDS)

ATGGAGAGTTCAATGAAGAAGACAATGAGCAACGGTAGTGTTCATATGGAGATGGAAGAAATTGACAATAAGTTTGAAGAAGCCTATGGAGAACACAATGCTACTGAAGATGAGCTCATTACTCCTTGGACAGTCTCTGTTGCTAGTGGTTATTCTTTACTGCGTGATCCGATCTACAACAAAGGCCTCGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCAGTTATTGATCAAGAGCTTCAGGAAAGGAGGATGATGCATAATCTTCGCAACTATGAAGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATTGATAACGTTGAAGAACTGCTTCCAATTGTGTACACTCCAACCGTAGGTGAAGCTTGCCAGAAGTATGGGAGCGTTTATAGACGACCTCAAGGTCTTTTCATCAGTTTGAAAGAGAAGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGAAACATTCAAGTTATTGTTGTTACCGACGGTGAGCGGATTCTAGGCCTTGGGGATCTTGGATGCCAGGGCATGGGGATTCCTGTGGGGAAACTTTCTTTATATACAGCACTTGGAGGAATTCGACCCTCAGCTTGCTTGCCTATAACCATTGATGTTGGTACAAACAACGAGCAGCTTCTAAATGATGAGTTTTACATTGGCCTTCGGCAGAAACGGGCCAGAGGACAGGAATACATGGAACTTTTAGACGAGTTCATGTATGCAGTAAAGAAGAACTATGGAGAGAAAGTTCTTATACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTCAATGACGACATTCAAGGTACAGCATCTGTTGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGGACATTAGCTGACCACACTTTCTTATTCCTTGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTCGTGGACTCGAAGGGGCTGATCGTTCAATCCCGCTTCGAATCCCTTCAACATTTTAAGAAGCCTTGGGCTCATGACCATGAATCTATCAAGGATCTTTACGGTGCCGTCCAGGCAATCAAACCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAGACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGAAACCACTTATCCTTGCTCTCTCGAACCCTACGTCTCAATCGGAGTGTACAGCTGAAGAAGCTTATACATGGAGCCAGGGCCGAGCAATCTTTGCTAGCGGAAGTCCATTTGACCCTGTTGAATACGATGGAAAAGTTTTTGTGCCTGGCCAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGTACAATTCGTGTACATGACGACATGCTCTTGGCAGCCTCGGAGGCTCTGGCTGCCCAAGTGAGTCAGGAAAACTATGATAAGGGATTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAACTTGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTTGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTATCGTAGCTACCTTTAA

Protein sequence

MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL*

Homology

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BLAST of Chy3G062440 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 7.5e-290
Identity = 496/587 (84.50%), Postives = 537/587 (91.48%), Query Frame = 0


Query: 2   ESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYNK 61
           E S+KK  SNG              + YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 12  EVSVKKDYSNG----------GGVRDLYGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 71

Query: 62  GLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLF 121
           GLAF+E ERDAHYLRGLLPP+V +QELQE+R+MHNLR YEVPLHRY+A+MDLQERNERLF
Sbjct: 72  GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 131

Query: 122 YKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQ 181
           YKLLIDNV ELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE++IQ
Sbjct: 132 YKLLIDNVAELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQ 191

Query: 182 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDE 241
           VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDE
Sbjct: 192 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDE 251

Query: 242 FYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 301
           FYIGLRQ+RA GQEY   LDEFM AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVF
Sbjct: 252 FYIGLRQRRATGQEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVF 311

Query: 302 NDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 361
           NDDIQGTASVV+AGLLA+LKL+GGTLADHTFLFLGAGEAGTGIAELIA+E+SKQT AP E
Sbjct: 312 NDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVE 371

Query: 362 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 421
           ETRKKIWLVDSKGLIV SR ESLQ FKKPWAH+HE +K L  AV+AIKPTVLIG+SG GK
Sbjct: 372 ETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGK 431

Query: 422 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 481
           TFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDPVEY+GK
Sbjct: 432 TFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGK 491

Query: 482 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 541
           +FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDKGLIYPPFTN
Sbjct: 492 LFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTN 551

Query: 542 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           IRKISANIAAKVAAKAY+LGLAS L RPKDLVK+AESCMYSP YRSY
Sbjct: 552 IRKISANIAAKVAAKAYDLGLASHLKRPKDLVKYAESCMYSPGYRSY 588

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BLAST of Chy3G062440 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 4.5e-279
Identity = 463/563 (82.24%), Postives = 527/563 (93.61%), Query Frame = 0


Query: 26  EEAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
           E+ YGE +ATED  ITPW+VSVASGYSLLRDP +NKGLAF+EKERDAHYLRGLLPP V++
Sbjct: 84  EDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPVVVN 143

Query: 86  QELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
            +LQ ++MMHN+R YEVPL RY AMMDLQERNERLFYKLLI+N+EELLPIVYTPTVGEAC
Sbjct: 144 HDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEELLPIVYTPTVGEAC 203

Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
           QKYG++++ PQGL+ISLK+KGK+LE+LKNWP++ IQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 204 QKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERILGLGDLGCQGMGIP 263

Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
           VGKLSLYTALGGIRPSACLPITIDVGTNNE++LNDEFYIGLRQ+RA G+EY EL++EFM 
Sbjct: 264 VGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEYAELMNEFMS 323

Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
           AVK+NYGEKVLIQFEDFANHNAF+LL +Y ++HLVFNDDIQGTASVV+AGL++ALKL+GG
Sbjct: 324 AVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGLISALKLVGG 383

Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
           +LADH FLFLGAGEAGTGIAELIALEISKQT AP EETRKKIWLVDSKGLIV+SR +SLQ
Sbjct: 384 SLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGLIVRSRLDSLQ 443

Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
           HFKKPWAHDHE +     AV+AIKPTVLIG+SG G+TFTKEVVEAM+SFNEKP+ILALSN
Sbjct: 444 HFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNEKPIILALSN 503

Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
           PTSQSECTAE+AYTWS+GR IFASGSPF PVEY+GKV+V GQ+NNAYIFPGFGLGLI+SG
Sbjct: 504 PTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAYIFPGFGLGLIISG 563

Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
            IRVHD+MLLAASEALA QV+QE++D GLIYPPFTNIRKISA+IAAKVAAKAYELGLASR
Sbjct: 564 AIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAKAYELGLASR 623

Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
           LP+P++LV +AESCMYSP+YR+Y
Sbjct: 624 LPQPENLVAYAESCMYSPKYRNY 646

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BLAST of Chy3G062440 vs. ExPASy Swiss-Prot
Match: Q9XGZ0 (NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME3 PE=1 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 1.1e-277
Identity = 465/562 (82.74%), Postives = 516/562 (91.81%), Query Frame = 0


Query: 27  EAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
           + YGE +AT D+L+TPW  SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP V+ Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85

Query: 87  ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
           KYGS+YRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGL+QKRA G+EY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCA 265

Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
           VK+NYGEKVL+QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +
Sbjct: 266 VKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKS 325

Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
           LADHTFLFLGAGEAGTGIAELIAL+ISK+TG P +ETRKKIWLVDSKGLIV  R ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQH 385

Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
           FK+PWAHDH+ +K+L  AV AIKPTVLIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNP 445

Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
           TSQ+ECTAEEAYTW++GRAIFASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG 
Sbjct: 446 TSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGA 505

Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
           IRV DDMLLAASEALA+QV++EN+  GLIYPPF NIRKISANIAA V AK YELGLAS L
Sbjct: 506 IRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNL 565

Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
           PRPKDLVK AESCMYSP YR++
Sbjct: 566 PRPKDLVKMAESCMYSPVYRNF 587

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BLAST of Chy3G062440 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 1.5e-277
Identity = 472/591 (79.86%), Postives = 537/591 (90.86%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEME---EIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDP 60
           MES++K      SV ++++    +    E+ YGE  ATED+L+TPWTVSVASGYSLLRDP
Sbjct: 1   MESTLKDIRDGASV-LDLDPKATVGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDP 60

Query: 61  IYNKGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERN 120
            +NKGLAF++KERDAHYL GLLPP V  QELQER++M+++R Y+VPL +Y+AMMDLQERN
Sbjct: 61  RHNKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERN 120

Query: 121 ERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPE 180
           ERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE
Sbjct: 121 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPE 180

Query: 181 RNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQL 240
           R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLPITIDVGTNNE+L
Sbjct: 181 RRIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKL 240

Query: 241 LNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSS 300
           L +EFYIGL+Q+RA G+EY E L EFM  VK+NYGEKVLIQFEDFANHNAF+LL++Y ++
Sbjct: 241 LANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTT 300

Query: 301 HLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTG 360
           HL FNDDIQGTASVV+AG+++AL+L+GGTLADH FLFLGAGEAGTGIAELIALE+SKQT 
Sbjct: 301 HLAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTK 360

Query: 361 APTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTS 420
            P EETRKKIWLVDSKGLIV SR +SLQ FKKPWAH+HE +KDL  AV+ IKPTVLIG+S
Sbjct: 361 CPIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSS 420

Query: 421 GVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVE 480
           GVGK FTKEV+EAMAS NEKPLILALSNPTSQSECTAEEAYTW+QGRAIFASGSPFDPVE
Sbjct: 421 GVGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVE 480

Query: 481 YDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYP 540
           Y+GK FVPGQANNAYIFPG G+GL++SG IRVHD+MLLAASEALA QV+QEN+DKGLIYP
Sbjct: 481 YNGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYP 540

Query: 541 PFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           PF+NIRKISA+IAA VAAKAYELGLA+RLP+P++LVK+AESCMYSP YRSY
Sbjct: 541 PFSNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSY 590

[Close]

BLAST of Chy3G062440 vs. ExPASy Swiss-Prot
Match: Q9LYG3 (NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 6.8e-275
Identity = 462/562 (82.21%), Postives = 516/562 (91.81%), Query Frame = 0


Query: 27  EAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
           + YGE +AT D+L+TPW  SVASGY+L+RDP YNKGLAF++KERDAHYL GLLPP ++ Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQ 85

Query: 87  ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
           KYGS++R+PQGL+ISL EKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLNDEFYIGL+Q+RA GQEY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCA 265

Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
           VK+NYGEKVL+QFEDFANHNAF+LLS+YS SHLVFNDDIQGTASVV+AGL+AA K++G  
Sbjct: 266 VKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKK 325

Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
           LADHTFLFLGAGEAGTGIAELIAL+ISK+TGAP  ETRKKIWLVDSKGLIV SR ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQH 385

Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
           FK+PWAH+H+ +KDL GAV AIKPTVLIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNP 445

Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
           TSQ+ECTAE+AYTW++GRAIF SGSPFDPV YDGK ++PGQANN YIFPG GLGLIMSG 
Sbjct: 446 TSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGA 505

Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
           IRV DDMLLAASEALAAQV++E+Y  GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS L
Sbjct: 506 IRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNL 565

Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
           PR KDLVKFAES MYSP YR+Y
Sbjct: 566 PRAKDLVKFAESSMYSPVYRNY 587

[Close]

BLAST of Chy3G062440 vs. ExPASy TrEMBL
Match: A0A0A0LLH9 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_2G373430 PE=3 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 589/589 (100.00%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHM+MEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589

[Close]

BLAST of Chy3G062440 vs. ExPASy TrEMBL
Match: A0A5A7VH34 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003800 PE=3 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 582/590 (98.64%), Postives = 588/590 (99.66%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQEL-QERRMMHNLRNYEVPLHRYIAMMDLQERNER 120
           KGLAFSEKERDAHYLRGLLPPA++DQEL QERRMMHNLRNYEVPLHRYIAMMDLQERNER
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPALLDQELQQERRMMHNLRNYEVPLHRYIAMMDLQERNER 120

Query: 121 LFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERN 180
           LFYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERN
Sbjct: 121 LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERN 180

Query: 181 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN 240
           IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN
Sbjct: 181 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN 240

Query: 241 DEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 300
           DEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL
Sbjct: 241 DEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 300

Query: 301 VFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 360
           VFNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP
Sbjct: 301 VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 360

Query: 361 TEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 420
            EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV
Sbjct: 361 IEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 420

Query: 421 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD 480
           GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD
Sbjct: 421 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD 480

Query: 481 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 540
           GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF
Sbjct: 481 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 540

Query: 541 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590

[Close]

BLAST of Chy3G062440 vs. ExPASy TrEMBL
Match: A0A1S3BB74 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103488170 PE=3 SV=1)

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 579/589 (98.30%), Postives = 587/589 (99.66%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 29  MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 88

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKER+AHYLRGLLPPA++DQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 89  KGLAFSEKERNAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 148

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 149 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 208

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 209 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 268

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 269 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 328

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 
Sbjct: 329 FNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPI 388

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 389 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 448

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 449 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 508

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           K FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 509 KAFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 568

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAA+VAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 569 NIRKISANIAAEVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 617

[Close]

BLAST of Chy3G062440 vs. ExPASy TrEMBL
Match: A0A6J1KT37 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111496169 PE=3 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 565/589 (95.93%), Postives = 582/589 (98.81%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           ME +MKKTMSNGSVHME+EEIDNK+EEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 18  MEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 77

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAF+EKERDAHYLRGLLPPA+++QELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 78  KGLAFTEKERDAHYLRGLLPPALLNQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 137

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 138 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 197

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 198 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 257

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLR KRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 258 EFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 317

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLL+ALKL+GGTLADHTFLFLGAGEAGTGIAELIALE+SKQT AP 
Sbjct: 318 FNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTQAPV 377

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIV+SRFESLQHFKKPWAHDHESIKDL GAVQAIKPTVLIGTSGVG
Sbjct: 378 EETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTVLIGTSGVG 437

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMAS NEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 438 KTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 497

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVS+ENY+KGLIYPPFT
Sbjct: 498 KVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSEENYEKGLIYPPFT 557

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSP YRSYL
Sbjct: 558 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPGYRSYL 606

[Close]

BLAST of Chy3G062440 vs. ExPASy TrEMBL
Match: A0A6J1GJD0 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111454390 PE=3 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 565/589 (95.93%), Postives = 582/589 (98.81%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           ME +MKKTMSNGSVHME+EEIDNK+EEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 18  MEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 77

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAF+EKERDAHYLRGLLPPA+++QELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 78  KGLAFTEKERDAHYLRGLLPPALLNQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 137

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 138 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 197

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 198 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 257

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLR KRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 258 EFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 317

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLL+ALKL+GGTLADHTFLFLGAGEAGTGIAELIALE+SKQT AP 
Sbjct: 318 FNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTQAPV 377

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIV+SRFESLQHFKKPWAHDHESIKDL GAVQAIKPTVLIGTSGVG
Sbjct: 378 EETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTVLIGTSGVG 437

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMAS NEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 438 KTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 497

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVS+ENY+KGLIYPPFT
Sbjct: 498 KVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSEENYEKGLIYPPFT 557

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSP YRSYL
Sbjct: 558 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPGYRSYL 606

[Close]

BLAST of Chy3G062440 vs. NCBI nr
Match: XP_011649727.1 (NADP-dependent malic enzyme isoform X1 [Cucumis sativus] >KGN63047.2 hypothetical protein Csa_022109 [Cucumis sativus])

HSP 1 Score: 1164 bits (3011), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 589/589 (100.00%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHM+MEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 28  MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 88  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 147

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 148 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 207

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 208 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 267

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 268 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 327

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 328 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 387

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 388 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 447

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 448 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 507

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 508 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 567

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 568 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 616

[Close]

BLAST of Chy3G062440 vs. NCBI nr
Match: XP_031737340.1 (NADP-dependent malic enzyme isoform X2 [Cucumis sativus])

HSP 1 Score: 1164 bits (3011), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 589/589 (100.00%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHM+MEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589

[Close]

BLAST of Chy3G062440 vs. NCBI nr
Match: KAA0065051.1 (NADP-dependent malic enzyme [Cucumis melo var. makuwa])

HSP 1 Score: 1155 bits (2988), Expect = 0.0
Identity = 582/590 (98.64%), Postives = 588/590 (99.66%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQ-ERRMMHNLRNYEVPLHRYIAMMDLQERNER 120
           KGLAFSEKERDAHYLRGLLPPA++DQELQ ERRMMHNLRNYEVPLHRYIAMMDLQERNER
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPALLDQELQQERRMMHNLRNYEVPLHRYIAMMDLQERNER 120

Query: 121 LFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERN 180
           LFYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERN
Sbjct: 121 LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERN 180

Query: 181 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN 240
           IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN
Sbjct: 181 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN 240

Query: 241 DEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 300
           DEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL
Sbjct: 241 DEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 300

Query: 301 VFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 360
           VFNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP
Sbjct: 301 VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 360

Query: 361 TEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 420
            EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV
Sbjct: 361 IEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 420

Query: 421 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD 480
           GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD
Sbjct: 421 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD 480

Query: 481 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 540
           GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF
Sbjct: 481 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 540

Query: 541 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
           TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590

[Close]

BLAST of Chy3G062440 vs. NCBI nr
Match: XP_008445000.2 (PREDICTED: NADP-dependent malic enzyme [Cucumis melo])

HSP 1 Score: 1155 bits (2987), Expect = 0.0
Identity = 579/589 (98.30%), Postives = 587/589 (99.66%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 29  MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 88

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKER+AHYLRGLLPPA++DQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 89  KGLAFSEKERNAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 148

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 149 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 208

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 209 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 268

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 269 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 328

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 
Sbjct: 329 FNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPI 388

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 389 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 448

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 449 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 508

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           K FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 509 KAFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 568

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
           NIRKISANIAA+VAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 569 NIRKISANIAAEVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 617

[Close]

BLAST of Chy3G062440 vs. NCBI nr
Match: XP_038884200.1 (NADP-dependent malic enzyme [Benincasa hispida])

HSP 1 Score: 1150 bits (2974), Expect = 0.0
Identity = 577/589 (97.96%), Postives = 584/589 (99.15%), Query Frame = 0


Query: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATED+LITPWTVSVASGYSLLRDPIYN
Sbjct: 28  MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPA++DQ  QERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 88  KGLAFSEKERDAHYLRGLLPPALLDQGRQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 147

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 148 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 207

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 208 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 267

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLR KRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 268 EFYIGLRHKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 327

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 
Sbjct: 328 FNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPI 387

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 388 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 447

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 448 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 507

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAA VSQENYDKGLIYPPFT
Sbjct: 508 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAHVSQENYDKGLIYPPFT 567

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
           NIRKISANIAAKVAAKAYELGLA+RLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 568 NIRKISANIAAKVAAKAYELGLATRLPRPKDLVKFAESCMYSPRYRSYL 616

[Close]

BLAST of Chy3G062440 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 956.8 bits (2472), Expect = 7.9e-279
Identity = 465/562 (82.74%), Postives = 516/562 (91.81%), Query Frame = 0


Query: 27  EAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
           + YGE +AT D+L+TPW  SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP V+ Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85

Query: 87  ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
           KYGS+YRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGL+QKRA G+EY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCA 265

Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
           VK+NYGEKVL+QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +
Sbjct: 266 VKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKS 325

Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
           LADHTFLFLGAGEAGTGIAELIAL+ISK+TG P +ETRKKIWLVDSKGLIV  R ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQH 385

Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
           FK+PWAHDH+ +K+L  AV AIKPTVLIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNP 445

Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
           TSQ+ECTAEEAYTW++GRAIFASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG 
Sbjct: 446 TSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGA 505

Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
           IRV DDMLLAASEALA+QV++EN+  GLIYPPF NIRKISANIAA V AK YELGLAS L
Sbjct: 506 IRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNL 565

Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
           PRPKDLVK AESCMYSP YR++
Sbjct: 566 PRPKDLVKMAESCMYSPVYRNF 587

[Close]

BLAST of Chy3G062440 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 947.6 bits (2448), Expect = 4.8e-276
Identity = 462/562 (82.21%), Postives = 516/562 (91.81%), Query Frame = 0


Query: 27  EAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
           + YGE +AT D+L+TPW  SVASGY+L+RDP YNKGLAF++KERDAHYL GLLPP ++ Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQ 85

Query: 87  ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
           KYGS++R+PQGL+ISL EKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLNDEFYIGL+Q+RA GQEY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCA 265

Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
           VK+NYGEKVL+QFEDFANHNAF+LLS+YS SHLVFNDDIQGTASVV+AGL+AA K++G  
Sbjct: 266 VKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKK 325

Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
           LADHTFLFLGAGEAGTGIAELIAL+ISK+TGAP  ETRKKIWLVDSKGLIV SR ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQH 385

Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
           FK+PWAH+H+ +KDL GAV AIKPTVLIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNP 445

Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
           TSQ+ECTAE+AYTW++GRAIF SGSPFDPV YDGK ++PGQANN YIFPG GLGLIMSG 
Sbjct: 446 TSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGA 505

Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
           IRV DDMLLAASEALAAQV++E+Y  GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS L
Sbjct: 506 IRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNL 565

Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
           PR KDLVKFAES MYSP YR+Y
Sbjct: 566 PRAKDLVKFAESSMYSPVYRNY 587

[Close]

BLAST of Chy3G062440 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 937.6 bits (2422), Expect = 5.0e-273
Identity = 455/563 (80.82%), Postives = 517/563 (91.83%), Query Frame = 0


Query: 26  EEAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
           ++ YGE  ATED  ITPW++SVASGY+LLRDP +NKGLAFS +ERDAHYLRGLLPP VI 
Sbjct: 83  QDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVIS 142

Query: 86  QELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
           Q+LQ +++MH LR Y+VPL +Y+AMMDLQE NERLFYKLLID+VEELLP++YTPTVGEAC
Sbjct: 143 QDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEAC 202

Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
           QKYGS++ RPQGLFISLKEKGKI EVL+NWPE+NIQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 203 QKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIP 262

Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
           VGKLSLYTALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGLRQ+RA G+EY EL+ EFM 
Sbjct: 263 VGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSELMHEFMT 322

Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
           AVK+NYGEKV+IQFEDFANHNAF+LL++Y ++HLVFNDDIQGTASVV+AGL+AAL+ +GG
Sbjct: 323 AVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAALRFVGG 382

Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
           +L+DH FLFLGAGEAGTGIAELIALEISK++  P EE RK IWLVDSKGLIV SR ES+Q
Sbjct: 383 SLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSSRKESIQ 442

Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
           HFKKPWAHDHE I++L  AV+AIKPTVLIGTSGVG+TFT++VVE MA  NEKP+IL+LSN
Sbjct: 443 HFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPIILSLSN 502

Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
           PTSQSECTAEEAYTWSQGRAIFASGSPF PVEY+GK FVPGQANNAYIFPGFGLGLIMSG
Sbjct: 503 PTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSG 562

Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
           TIRVHDDMLLAASEALA ++ +E+Y+KG+IYPPF NIRKISA IAAKVAAKAYELGLA+R
Sbjct: 563 TIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYELGLATR 622

Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
           LP+PK+L + AES MYSP YRSY
Sbjct: 623 LPQPKELEQCAESSMYSPSYRSY 645

[Close]

BLAST of Chy3G062440 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 934.1 bits (2413), Expect = 5.5e-272
Identity = 454/568 (79.93%), Postives = 519/568 (91.37%), Query Frame = 0


Query: 21  IDNKFEEAYGEHNATEDELITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLP 80
           +D    + YGE +AT +  ITPW++SV+SGYSLLRDP YNKGLAF+EKERD HYLRGLLP
Sbjct: 13  VDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLP 72

Query: 81  PAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPT 140
           P V+DQ+LQE+R+++N+R Y+ PL +Y+A+ +LQERNERLFYKLLIDNVEELLPIVYTPT
Sbjct: 73  PVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPT 132

Query: 141 VGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQ 200
           VGEACQK+GS++RRPQGLFISLK+KGKIL+VLKNWPERNIQVIVVTDGERILGLGDLGCQ
Sbjct: 133 VGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQ 192

Query: 201 GMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELL 260
           GMGIPVGKL+LY+ALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGLRQKRA GQEY ELL
Sbjct: 193 GMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELL 252

Query: 261 DEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAAL 320
           +EFM AVK+NYGEKVLIQFEDFANHNAFELL++YS +HLVFNDDIQGTASVV+AGL++A 
Sbjct: 253 NEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQ 312

Query: 321 KLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSR 380
           KL    LA+HTFLFLGAGEAGTGIAELIAL +SKQ  A  EE+RKKIWLVDSKGLIV SR
Sbjct: 313 KLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVDSKGLIVNSR 372

Query: 381 FESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLI 440
            +SLQ FKKPWAH+HE +KDL GA++AIKPTVLIG+SGVG++FTKEV+EAM+S NE+PLI
Sbjct: 373 KDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAMSSINERPLI 432

Query: 441 LALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLG 500
           +ALSNPT+QSECTAEEAYTWS+GRAIFASGSPFDPVEY+GKVFV  QANNAYIFPGFGLG
Sbjct: 433 MALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLG 492

Query: 501 LIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYEL 560
           L++SG IRVHDDMLLAA+EALA QVS+ENY+KG+IYP F++IRKISA IAA VA KAYEL
Sbjct: 493 LVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYEL 552

Query: 561 GLASRLPRPKDLVKFAESCMYSPRYRSY 589
           GLA RLPRPKD+VK AES MYSP YR Y
Sbjct: 553 GLAGRLPRPKDIVKCAESSMYSPTYRLY 580

[Close]

BLAST of Chy3G062440 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.6e-122
Identity = 246/578 (42.56%), Postives = 356/578 (61.59%), Query Frame = 0


Query: 44  TVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEV- 103
           T+    G  +L DP +NKG AF+  ER+   LRGLLPP V+D E Q  R M +L+  E  
Sbjct: 40  TIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQ 99

Query: 104 ---------PLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYR 163
                     L ++  +  L +RNE ++YK+LI+N+EE  PIVYTPTVG  CQ Y  ++R
Sbjct: 100 ARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159

Query: 164 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 223
           RP+G++ S +++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI VGKL LY 
Sbjct: 160 RPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYV 219

Query: 224 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGE 283
           A  GI P   LP+ IDVGTNNE+L ND  Y+GL+Q+R    +Y++++DEFM AV   +  
Sbjct: 220 AAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-P 279

Query: 284 KVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADH--- 343
            V++QFEDF +  AF+LL RY  ++ +FNDD+QGTA V +AGLL A++  G  + D    
Sbjct: 280 HVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKM 339

Query: 344 TFLFLGAGEAGTGIAELIALEISKQTG---APTEETRKKIWLVDSKGLIVQSRFESLQHF 403
             +  GAG AG G+       +++  G      +  + + W+VD++GLI + R E++   
Sbjct: 340 KIVVAGAGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGR-ENIDPE 399

Query: 404 KKPWAH-----DHESIKD---LYGAVQAIKPTVLIGTSGVGKTFTKEVVEAM-ASFNEKP 463
            +P+A      + + +K+   L   V+ +KP VL+G S VG  F+KEV+EAM  S + +P
Sbjct: 400 AQPFARKTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRP 459

Query: 464 LILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEY-DGKVFVPGQANNAYIFPGF 523
            I A+SNPT  +ECT ++A++      IFASGSPF  VE+ +G V    Q NN Y+FPG 
Sbjct: 460 AIFAMSNPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGI 519

Query: 524 GLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKA 583
           GLG ++SG   V D ML AASE LAA +S+E   +G+IYPP + IR I+  IAA V  +A
Sbjct: 520 GLGTLLSGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEA 579

Query: 584 YELGL--------ASRLPR--PKDLVKFAESCMYSPRY 586
            E  L        A  + +   + L+++ E+ M++P Y
Sbjct: 580 IEEDLVEGYREMDAREIQKLDEEGLMEYVENNMWNPEY 615

The following BLAST results are available for this feature:

BLAST of Chy3G062440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P12628	7.5e-290	84.50	NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1	[more]
P36444	4.5e-279	82.24	NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ...	[more]
Q9XGZ0	1.1e-277	82.74	NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME3 PE=1 S...	[more]
P51615	1.5e-277	79.86	NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1	[more]
Q9LYG3	6.8e-275	82.21	NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 S...	[more]

BLAST of Chy3G062440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LLH9	0.0e+00	99.66	Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_2G373430 PE=3 SV=1	[more]
A0A5A7VH34	0.0e+00	98.64	Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003800 P...	[more]
A0A1S3BB74	0.0e+00	98.30	Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103488170 PE=3 SV=1	[more]
A0A6J1KT37	0.0e+00	95.93	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111496169 PE=3 SV=1	[more]
A0A6J1GJD0	0.0e+00	95.93	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111454390 PE=3 SV=1	[more]

BLAST of Chy3G062440 vs. NCBI nr


Match Name	Identity	Description
XP_011649727.1	99.66	NADP-dependent malic enzyme isoform X1 [Cucumis sativus] >KGN63047.2 hypothetica...	[more]
XP_031737340.1	99.66	NADP-dependent malic enzyme isoform X2 [Cucumis sativus]	[more]
KAA0065051.1	98.64	NADP-dependent malic enzyme [Cucumis melo var. makuwa]	[more]
XP_008445000.2	98.30	PREDICTED: NADP-dependent malic enzyme [Cucumis melo]	[more]
XP_038884200.1	97.96	NADP-dependent malic enzyme [Benincasa hispida]	[more]

BLAST of Chy3G062440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25880.1	7.9e-279	82.74	NADP-malic enzyme 3	[more]
AT5G11670.1	4.8e-276	82.21	NADP-malic enzyme 2	[more]
AT1G79750.1	5.0e-273	80.82	NADP-malic enzyme 4	[more]
AT2G19900.1	5.5e-272	79.93	NADP-malic enzyme 1	[more]
AT2G13560.1	1.6e-122	42.56	NAD-dependent malic enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 18..38
None	No IPR available	GENE3D	3.40.50.720		coord: 303..589 e-value: 1.9E-122 score: 410.3
None	No IPR available	PIRSR	PIRSR000106-3	PIRSR000106-3	coord: 52..388 e-value: 2.0E-141 score: 470.0
None	No IPR available	PANTHER	PTHR23406:SF65	MALIC ENZYME	coord: 5..588
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 5..588
None	No IPR available	CDD	cd05312	NAD_bind_1_malic_enz	coord: 305..583 e-value: 3.24721E-147 score: 423.883
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 28..304
IPR001891	Malic oxidoreductase	PRINTS	PR00072	MALOXRDTASE	coord: 120..144 score: 70.8 coord: 216..238 score: 67.72 coord: 435..451 score: 67.06 coord: 332..348 score: 71.32 coord: 276..294 score: 67.76 coord: 180..209 score: 82.75 coord: 301..317 score: 78.97
IPR001891	Malic oxidoreductase	PIRSF	PIRSF000106	ME	coord: 9..589 e-value: 1.6E-235 score: 781.0
IPR012302	Malic enzyme, NAD-binding	SMART	SM00919	Malic_M_2	coord: 305..558 e-value: 5.1E-115 score: 398.1
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 305..557 e-value: 9.1E-98 score: 326.6
IPR012301	Malic enzyme, N-terminal domain	SMART	SM01274	malic_2	coord: 114..295 e-value: 3.7E-102 score: 355.3
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 114..294 e-value: 5.5E-80 score: 267.5
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	3.40.50.10380		coord: 48..302 e-value: 2.1E-126 score: 422.2
IPR015884	Malic enzyme, conserved site	PROSITE	PS00331	MALIC_ENZYMES	coord: 301..317
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 305..588

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G062440.1	Chy3G062440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0004470	malic enzyme activity