Chy3G062220 (gene) Cucumber (hystrix) v1

BLAST of Chy3G062220 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 2.0e-143
Identity = 269/558 (48.21%), Postives = 373/558 (66.85%), Query Frame = 0

Query: 13  KGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPII 72
           KGG+ T+PFIIA+ A EK+AS GL P+MI+YL   Y       +NV+F+W+AA+NFTP++
Sbjct: 22  KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLL 81

Query: 73  CAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPF-CDEIS--GHCDAPSTPQL 132
            AFL+DSY GRF  I+  S+ S LGM +LWLTA++P+ +P  CD  +   HC + +  QL
Sbjct: 82  GAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQL 141

Query: 133 LLLYSSYIVMSIGSGCVQASCPVFGADQL-YREKKSNIGILDRYFNIFYISTAVGALVGM 192
            LLYS++ ++SIGSG ++     FGADQL  +E   N  +L+ +F  +Y S+AV  L+  
Sbjct: 142 ALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAF 201

Query: 193 SCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKK 252
           + IVYIQ+ +GW IGFGV   LML+A++ F+ ASPLY+    ++S   GL Q I  AYKK
Sbjct: 202 TGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKK 261

Query: 253 RHTQIP-FVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKASNPWSLCT 312
           R   +P   D+ + Y+H   S    PS KLRFLNKAC+I N EEE+ S G A NPW LCT
Sbjct: 262 RKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCT 321

Query: 313 VEQVENLKALIRIIPLWSTEILVAACLHQ-SFYVLQVASMDRHLT---PGFEVPAGSFTG 372
            ++VE LKALI++IP+WST I+++    Q SF +LQ  SMDR L+     F+VPAGSF  
Sbjct: 322 TDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGM 381

Query: 373 MILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRRA 432
             ++ L +W+ LYDR ++PLASK RG+P RLS K +MG+G+    L++A+SA+VE  RR 
Sbjct: 382 FTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRK 441

Query: 433 LAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISLL 492
            AI +G+++N NAVV +SA W +P+Y+  G+ EA + I Q EFFY E PK+MSS+A SL 
Sbjct: 442 KAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLF 501

Query: 493 GLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYLA 552
           GL     +L AS ++  V+ L+   G +SWVS NIN+GH +YYYW+L  +   N  YY+ 
Sbjct: 502 GLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVI 561

Query: 553 CNKSYGPSKEE-SEGRSN 561
           C+ SYGP  ++   GR N
Sbjct: 562 CSWSYGPLVDQVRNGRVN 579

BLAST of Chy3G062220 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 497.3 bits (1279), Expect = 2.3e-139
Identity = 266/549 (48.45%), Postives = 362/549 (65.94%), Query Frame = 0

Query: 13  KGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPII 72
           KGGL T+PFIIA+   EK+AS GL  +MILYL   Y +  V    V+F+W AATNF P++
Sbjct: 24  KGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLV 83

Query: 73  CAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPF-CDEISG-HCDAPSTPQLL 132
            AFL+DSY GRF  I   S+ S LGM VLWLTA++P+ +P  C   +G +C + ++ QL 
Sbjct: 84  GAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLA 143

Query: 133 LLYSSYIVMSIGSGCVQASCPVFGADQL-YREKKSNIGILDRYFNIFYISTAVGALVGMS 192
           LLY+++ ++SIGSG ++     FGADQL  +E   N  +L+ +F  +Y S++V  L+  +
Sbjct: 144 LLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFT 203

Query: 193 CIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKKR 252
            IVYIQD +GW IGFG+   LMLLA   F+ ASPLY+K   S+S   GL QV+  AY KR
Sbjct: 204 VIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKR 263

Query: 253 HTQIP-FVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKASNPWSLCTV 312
           +  +P   D+ + Y+    S    PSDKLRFLNKAC I N +E+L S G A N W LCT 
Sbjct: 264 NLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTT 323

Query: 313 EQVENLKALIRIIPLWSTEILVAACLHQ-SFYVLQVASMDRHLTPG--FEVPAGSFTGMI 372
           +QVE LKAL+++IP+WST I+++  + Q SF +LQ  SMDR L+    F++PAGSF    
Sbjct: 324 DQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFT 383

Query: 373 LVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRRALA 432
           ++ LI W+ LYDR ILPLASK RG+P R++ K +MG+G+    L++AVSA VE  RR  A
Sbjct: 384 IIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTA 443

Query: 433 IKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISLLGL 492
           I +G +++ N+ VS+SA W +P+Y+  G+ EA + I Q EFFY E PK+MSS+A SL GL
Sbjct: 444 ISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGL 503

Query: 493 SSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYLACN 552
                N+ AS I+  V N SK  G  SW+  NIN+GH DYYYW+L  L   N  YY+ C+
Sbjct: 504 GMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCS 563

Query: 553 KSYGPSKEE 555
            SYGP+ ++
Sbjct: 564 WSYGPTVDQ 571

BLAST of Chy3G062220 vs. ExPASy Swiss-Prot
Match: Q9LYD5 (Protein NRT1/ PTR FAMILY 1.3 OS=Arabidopsis thaliana OX=3702 GN=NPF1.3 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.2e-98
Identity = 225/555 (40.54%), Postives = 306/555 (55.14%), Query Frame = 0

Query: 2   EEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFL 61
           +E LL      K G+ T+PFI+AS ALEKLA  GL P+MIL+LT  YGM +  A+N++FL
Sbjct: 3   QEALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILFL 62

Query: 62  WTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGHC 121
           W+AATNF P++ AF+ADSY GRFP+I  GS IS  GM +LWLT II   RP CD+++  C
Sbjct: 63  WSAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNVC 122

Query: 122 DAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNI--GILDRYFNIFYIS 181
              +  + +LLYS + + +IG+G V++SC  F ADQL   + S +    L+  FN +Y S
Sbjct: 123 QPTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYFS 182

Query: 182 TAVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLM-PSRSWCVGL 241
             V   +  S +V++Q   GW IGFGVSVA M L+   F +ASP Y++   P+R+     
Sbjct: 183 VMVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYVRFQKPTRN----- 242

Query: 242 VQVIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGK 301
                                                                       
Sbjct: 243 ------------------------------------------------------------ 302

Query: 302 ASNPWSLCTVEQVENLKALIRIIPLWSTEI---LVAACLHQSFYVLQVASMDRH-LTPGF 361
           + NPW LC V+QVE+LK+LI +IP+WST I   LV AC   SF VLQ  +MDRH    GF
Sbjct: 303 SRNPWKLCRVQQVEDLKSLINVIPIWSTGIILSLVTAC-QVSFIVLQAKTMDRHTFIQGF 362

Query: 362 EVPAGSFTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVS 421
           E+P GS+   +++  ++++ LYD +I+PL S    +P RL    +M  G +   L ++  
Sbjct: 363 EIPPGSYGIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISAL 422

Query: 422 AIVEGNRRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKA 481
           A  E  RR  A  E       +   +SA W LP  I  G+ EA + IAQ EFFY ELPK 
Sbjct: 423 AATEYARRKTARDE-------SGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKT 477

Query: 482 MSSVAISLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLL 541
           MSSVA +L  L+  A +L +S+I+T VD         SW++ NI+EGH DYYYWLL GL 
Sbjct: 483 MSSVATTLSSLNMAAASLISSWIITIVD----VTTYGSWITENIDEGHLDYYYWLLVGLS 477

Query: 542 VANFFYYLACNKSYG 550
           + N  Y++ C KSYG
Sbjct: 543 LLNVLYFVWCKKSYG 477

BLAST of Chy3G062220 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 8.4e-89
Identity = 194/569 (34.09%), Postives = 317/569 (55.71%), Query Frame = 0

Query: 14  GGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPIIC 73
           GG R + FI+ +  LE+L S+GL  + ++YLT+V+ ++ V A+NVI +W+  TN TP++ 
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 74  AFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFC----DEISGHCDAPSTPQL 133
           A+++D+Y GRF  IA  S  + LG+  + LTA  P+  P      D +S  C  P+  Q+
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPNKLQI 172

Query: 134 LLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYISTAVGALVGMS 193
            +L      +S+GSG ++     FG DQ  +  +  +  +  +FN +Y++  V  ++  +
Sbjct: 173 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 232

Query: 194 CIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKKR 253
            +VYIQD++ W IGF +   LM LA + F +    Y+ + P  S   G+ QVI  A KKR
Sbjct: 233 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 292

Query: 254 HTQIPFVDTSEMYHHENGSPCTL-----PSDKLRFLNKACIIKNAEEELTSYGKASNPWS 313
             ++P  D   + +++     ++      S++ R L+KA ++   E +LT  G  ++ W 
Sbjct: 293 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 314 LCTVEQVENLKALIRIIPLWSTEI--LVAACLHQSFYVLQVASMDRHLTPGFEVPAGSFT 373
           LC+V++VE +K LIRI+P+WS  I  L A     +F V Q   MDR+L P FE+PAGS +
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 374 GMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRR 433
            + L+ + I++  YDR+ +P   +  G  + ++   ++G GI+F   S+ V+ IVE  RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 434 ALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISL 493
             +I  G   +P  +  MS FW  P+ I +G+ EAF++I Q+EFF  + P+ M S+A SL
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 494 LGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYL 553
             LS    +  +SF++T V   S       W++ N+N G  DY+Y+L+  L V N  Y+ 
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFW 592

Query: 554 ACNKSY----GPSKEESEGRSNAEDSNKT 568
            C + Y    G   E+ E   +++D   T
Sbjct: 593 YCARGYRYKVGLPIEDFEEDKSSDDVEMT 614

BLAST of Chy3G062220 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 320.9 bits (821), Expect = 3.0e-86
Identity = 187/565 (33.10%), Postives = 312/565 (55.22%), Query Frame = 0

Query: 15  GLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPIICA 74
           G + +PFII +   EKL  +G   ++++YLT V+ +KS  A+ +I  ++   NF   I A
Sbjct: 64  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAA 123

Query: 75  FLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGHCDAPSTPQLLLLYS 134
           FL D+YFGR+  ++   I   LG FV+ LTA IP   P        C+ PS  Q+L L  
Sbjct: 124 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKISCEGPSVGQILFLLM 183

Query: 135 SYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYISTAVGALVGMSCIVYI 194
               + +G+G ++     FGADQ   + +S    ++ +FN ++ +     ++ ++ +VYI
Sbjct: 184 GLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYI 243

Query: 195 QDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKKRHTQIP 254
           Q  + W IG  + VALM LA + F +   LY+K+  S S   G+ +VI  A KKR  +  
Sbjct: 244 QSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPV 303

Query: 255 FVDTSEMYHH--ENGSPCTLP-SDKLRFLNKACIIKNAEEELTSYGKASNPWSLCTVEQV 314
                 +Y+H   N +  TL  +D+ RFL+KA I+   EE+L S G AS+PW LCT++QV
Sbjct: 304 KQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIM-TPEEKLNSDGTASDPWKLCTLQQV 363

Query: 315 ENLKALIRIIPLW--STEILVAACLHQSFYVLQVASMDRHL-TPGFEVPAGSFTGMILVF 374
           E +K ++R+IP+W  ST   +A  +  ++ V Q    DR L + GF +PA ++   ++  
Sbjct: 364 EEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTG 423

Query: 375 LIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRRALAIKE 434
           + ++I  YDR+++P   +  G  T +S   ++G G  F  +SL VS  +E  RR  A+ +
Sbjct: 424 MTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTK 483

Query: 435 ---GFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISLLGL 494
              G +     + SMSA W +P+    G+ EAF+ I Q+EF+Y + P+ M S A S+  +
Sbjct: 484 PTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYV 543

Query: 495 SSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYLACN 554
            +   +  ASF+++TV   +      +W++ ++N+   DY+Y++L GL+V N  Y+L   
Sbjct: 544 GAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTGLMVVNMAYFLLMA 603

Query: 555 KSY----GPSKEESEGRSNAEDSNK 567
           + Y    G  ++ +E  +N E++ +
Sbjct: 604 RWYRYKGGNDEDITEIETNEEETKQ 627

BLAST of Chy3G062220 vs. ExPASy TrEMBL
Match: A0A0A0LPT0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G374640 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 3.0e-270
Identity = 476/569 (83.66%), Postives = 507/569 (89.10%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLS TD  KGG+RTLPFIIASGALEKLAS+GLSPSMILYLTEVYGMKSV AS+VIF
Sbjct: 13  MEEPLLSGTDDCKGGIRTLPFIIASGALEKLASMGLSPSMILYLTEVYGMKSVQASSVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LWTAAT FTPIICAFLADSYFGRF MI AGSI SCLGMFVLWLTA+IP+ARPFCDEISGH
Sbjct: 73  LWTAATYFTPIICAFLADSYFGRFMMIVAGSIFSCLGMFVLWLTAMIPQARPFCDEISGH 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           C+APSTPQLL LYSSY +MSIGSGC+Q+S   FGADQLY+E KSN GILD YFN+ YIS 
Sbjct: 133 CNAPSTPQLLFLYSSYAIMSIGSGCLQSSNFAFGADQLYKENKSNSGILDIYFNLCYISV 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           +VG LVGMSCIVYIQDRMGW +GFGV +ALMLLA++TFLSAS LYLK +PS+SWC GLV 
Sbjct: 193 SVGTLVGMSCIVYIQDRMGWEMGFGVPIALMLLATVTFLSASSLYLKSVPSKSWCAGLVH 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F AYKKR  QIPFV TSEMYHHENGS CTLPSDKLRFLNKACIIKN EEEL S GKAS
Sbjct: 253 VVFAAYKKRRMQIPFVGTSEMYHHENGSSCTLPSDKLRFLNKACIIKNYEEELISDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALIRIIPLWST ILV+A + QS YVLQVASMDRHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIRIIPLWSTGILVSASMSQSVYVLQVASMDRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F  M++VF+IIWI LYDRLILPLAS+YRGKPTRLS KT+MG+ I FC LSLAVSAIVEG 
Sbjct: 373 FIAMLVVFVIIWIILYDRLILPLASRYRGKPTRLSGKTRMGMAIPFCILSLAVSAIVEGY 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAIKEGFSD+PNAVVSMSAFWTLPRYI  G+ EAF  I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIKEGFSDDPNAVVSMSAFWTLPRYILFGIAEAFYAIGQLEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGLSSCAGNLAASFIMTTVDN SK +GVKSWVSSNINEGHNDYYYWLLFGLLVANFFY
Sbjct: 493 SLLGLSSCAGNLAASFIMTTVDNFSKTIGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 570
           YLACN SYGPSKEESE  SNAED N TVN
Sbjct: 553 YLACNNSYGPSKEESEDISNAEDYNNTVN 581

BLAST of Chy3G062220 vs. ExPASy TrEMBL
Match: A0A0A0LLZ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G374650 PE=3 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.5e-269
Identity = 473/569 (83.13%), Postives = 510/569 (89.63%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLSDTD  KGG RTLPFIIA+GALEKLAS GLSPSMILYLT+VYGMKS H+SNVIF
Sbjct: 13  MEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHSSNVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LW+AA+NFTPIICAFLADSYFGRFPMIAAGSI S LGMFVLWLTA+IP+ARPFCDEISGH
Sbjct: 73  LWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISGH 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           CDAPST QLLLLYSSY +MS+GSGC+QAS   FGADQLYR+ KSN GILD YFN+ YIS 
Sbjct: 133 CDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISA 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           A+G LVGMSCIVYIQDRMGWG+GFGV VALM LA+ITFLSASPLYLK  PS+SWC GLVQ
Sbjct: 193 ALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQ 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F AYKKRHTQIPFV TSEMYHHEN SPCTLPS+KLRFLNKACII+N++EELT  GKAS
Sbjct: 253 VVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRNSKEELTLDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALIRIIPLWST ILV+A L QSFYVLQVASM+RHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F+ +I+V LIIWI LYDRLILPLASK RGKPTRL AKT+MG+GIL C LSLAVSAIVEG+
Sbjct: 373 FSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCGLSLAVSAIVEGD 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAIKEGFSDNPNAVVSMSAFWTLPRYI  G+ EA + I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL+S  GNLAASFIMTTVDNLSK+VGVKSWVSSNINEGH+DYYYWLL GLL ANF Y
Sbjct: 493 SLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 570
           YLAC+KSYGPS EES GR+NAED   T N
Sbjct: 553 YLACSKSYGPSNEESVGRANAEDYKNTAN 581

BLAST of Chy3G062220 vs. ExPASy TrEMBL
Match: A0A1S3BBQ7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488189 PE=3 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 5.2e-267
Identity = 469/569 (82.43%), Postives = 507/569 (89.10%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLSDTD GKGG+RTLPFIIA+GALEKLAS GLSPSMILYLT+VYGMKS  ASNVIF
Sbjct: 13  MEEPLLSDTDDGKGGVRTLPFIIANGALEKLASQGLSPSMILYLTQVYGMKSAQASNVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LW+AA+NFTPIICAFLADSYFGRFPM+AAGSI S LGMFVLWLT +IP+ARP CDEI+GH
Sbjct: 73  LWSAASNFTPIICAFLADSYFGRFPMVAAGSIFSFLGMFVLWLTVMIPQARPLCDEINGH 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           C+APS PQLLLLYSSY +MSIGSGC+QAS   FGADQLYR+ KS  GILD YFNI YIS 
Sbjct: 133 CNAPSIPQLLLLYSSYAIMSIGSGCLQASYLAFGADQLYRKNKSKSGILDNYFNICYISA 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           A+G LVGMSCIVYIQDRMGWG+GFGV V LMLLA+ITFLSAS LYLK MPS+SWC GLVQ
Sbjct: 193 ALGTLVGMSCIVYIQDRMGWGMGFGVPVVLMLLATITFLSASSLYLKSMPSKSWCAGLVQ 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F AYKKRH QI FV TS MYHHENGS C LPSDKLRFLNKACII+N+EEELTS GKAS
Sbjct: 253 VVFAAYKKRHMQIAFVGTSGMYHHENGSLCALPSDKLRFLNKACIIRNSEEELTSDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALI+IIPLWST ILV+A L+QSFYVLQ+ASMDRHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIKIIPLWSTGILVSASLNQSFYVLQLASMDRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F  M++V LIIWITLYDRLILPLASK RGKPTRLSAKT+MG+GIL CTLSLAVSA+VEG 
Sbjct: 373 FGAMLVVSLIIWITLYDRLILPLASKCRGKPTRLSAKTRMGLGILCCTLSLAVSAVVEGY 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAI+EGFSD+PNAVVSMSAFWTLPRYI  GM EAF+ I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIREGFSDDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL+S  GNLAASFIMTTVDN SKAVG KSWVSSNIN+GH+DYYYWLLFGLL AN  Y
Sbjct: 493 SLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYWLLFGLLFANLLY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 570
           +LAC+KSYGPSKEES GRS AED N  VN
Sbjct: 553 FLACSKSYGPSKEESGGRSTAEDYNNAVN 581

BLAST of Chy3G062220 vs. ExPASy TrEMBL
Match: A0A1S4DVD3 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103488191 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.0e-266
Identity = 471/569 (82.78%), Postives = 506/569 (88.93%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLSDTD GKGGLRTLPFIIA+G  EK+AS GLSPSMILYLTEVYGMKS HASNVIF
Sbjct: 13  MEEPLLSDTDDGKGGLRTLPFIIANGTFEKVASHGLSPSMILYLTEVYGMKSAHASNVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LW+AATNFTPIICAFLADSYFGRF MIAAGSI S LGMFVLWLTAIIP+ RPFCD+I+G+
Sbjct: 73  LWSAATNFTPIICAFLADSYFGRFSMIAAGSIFSFLGMFVLWLTAIIPQVRPFCDKINGY 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           CDAPSTPQLLLLYSSY +MSIGSGC+QAS   FGADQLY+E KSN GIL+ YFNI YIS 
Sbjct: 133 CDAPSTPQLLLLYSSYAIMSIGSGCLQASHLAFGADQLYKESKSNSGILNSYFNICYISI 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           AVG L+GMSCIVYIQDRMGWGIGFGVSVALMLLA+ITFLSAS LYLK MPS+SWC GLVQ
Sbjct: 193 AVGTLIGMSCIVYIQDRMGWGIGFGVSVALMLLATITFLSASSLYLKSMPSKSWCDGLVQ 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F  YKKRH QIPFV T EMYHHE GS C LPS+KLRFLNKACII+N EEELTS GKAS
Sbjct: 253 VVFATYKKRHMQIPFVGTFEMYHHETGSLCALPSNKLRFLNKACIIRNFEEELTSDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALIRIIPLWST IL +  L QSFYVLQVASMDRHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIRIIPLWSTGILFSVSLSQSFYVLQVASMDRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F+ +I+VF+IIWI LY+ LILPLASK RGKPT LS KT+MG+GIL CTLSLAVSAIVEG+
Sbjct: 373 FSVLIVVFVIIWIILYNCLILPLASKCRGKPTHLSGKTRMGLGILCCTLSLAVSAIVEGD 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAIKEGFS++PNAVVSMSAFWTLPRYI  GM EAF+ I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIKEGFSNDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL+S  GNLAASFIMTTVDN SKAVG KSWVSSNIN+GH+DYYYWLLFGLL ANF Y
Sbjct: 493 SLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYWLLFGLLFANFLY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 570
           +LAC+KSYGPSKEES GRS AED N TVN
Sbjct: 553 FLACSKSYGPSKEESAGRSTAEDYNNTVN 581

BLAST of Chy3G062220 vs. ExPASy TrEMBL
Match: A0A1S3BCH0 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103488190 PE=3 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 6.4e-249
Identity = 440/530 (83.02%), Postives = 474/530 (89.43%), Query Frame = 0

Query: 40  MILYLTEVYGMKSVHASNVIFLWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMF 99
           MILYLTEVYGMKS  ASNVIFLW+AATNFTPI+CAFLADSYFGRFPM+AAGSI S LG  
Sbjct: 1   MILYLTEVYGMKSAQASNVIFLWSAATNFTPIVCAFLADSYFGRFPMVAAGSIFSFLGTI 60

Query: 100 VLWLTAIIPRARPFCDEISGHCDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLY 159
           VLWLTA+IP+ARPFCDEISG+CDAPSTPQLLLLYSSY ++SIGSGCVQAS   FGADQLY
Sbjct: 61  VLWLTAMIPQARPFCDEISGYCDAPSTPQLLLLYSSYAIISIGSGCVQASYIAFGADQLY 120

Query: 160 REKKSNIGILDRYFNIFYISTAVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFL 219
           R+ KSN GILD YFN  YIS A+G LVGMSCIVYIQDR+GWG+GFGV VALMLLA+ITF 
Sbjct: 121 RKNKSNSGILDSYFNTCYISAALGTLVGMSCIVYIQDRIGWGMGFGVPVALMLLATITFF 180

Query: 220 SASPLYLKLMPSRSWCVGLVQVIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRF 279
            ASPLYLK MPS SWC GLVQV+F AYKKRH QIPFV TSE+YH ENGS C LPSDKLRF
Sbjct: 181 LASPLYLKSMPSESWCAGLVQVVFAAYKKRHMQIPFVGTSEIYHQENGSSCALPSDKLRF 240

Query: 280 LNKACIIKNAEEELTSYGKASNPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFY 339
            NKACII+N+EEELT  GKASNPWSL TVEQVENLKAL+RIIPLWST ILV+A L+QSFY
Sbjct: 241 FNKACIIRNSEEELTPDGKASNPWSLSTVEQVENLKALMRIIPLWSTGILVSASLNQSFY 300

Query: 340 VLQVASMDRHLTPGFEVPAGSFTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTK 399
           VLQVASMDRHLT  FEVPAGSF+ M+LV LIIWITLYDRLILPLASK RGKPTRLSAKT+
Sbjct: 301 VLQVASMDRHLTSSFEVPAGSFSMMVLVLLIIWITLYDRLILPLASKCRGKPTRLSAKTR 360

Query: 400 MGIGILFCTLSLAVSAIVEGNRRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFS 459
           +GIGIL CTLSLAVSAIVE +RRALAIKEGFSDNPNAVVSMSAFWTLPRYI LG+EE FS
Sbjct: 361 IGIGILCCTLSLAVSAIVESDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILLGIEEGFS 420

Query: 460 MIAQVEFFYFELPKAMSSVAISLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNIN 519
           +IAQVEFFY ELPKAMSSVA SLLGL+S  GNLAASFIMTTVDNLS+AVG +SWVSSNIN
Sbjct: 421 VIAQVEFFYNELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNLSEAVGGRSWVSSNIN 480

Query: 520 EGHNDYYYWLLFGLLVANFFYYLACNKSYGPSKEESEGRSNAEDSNKTVN 570
           EGH+DYYYWLLFGLL ANF Y+LAC+KSYGPSKEES GRS+AED    VN
Sbjct: 481 EGHSDYYYWLLFGLLFANFLYFLACSKSYGPSKEESAGRSSAEDYKNIVN 530

BLAST of Chy3G062220 vs. NCBI nr
Match: XP_011649735.2 (protein NRT1/ PTR FAMILY 1.2 [Cucumis sativus] >KAE8652164.1 hypothetical protein Csa_021897 [Cucumis sativus])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 474/569 (83.30%), Postives = 507/569 (89.10%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLS TD  KGG+RTLPFIIASGALEKLAS+GLSPSMILYLTEVYGMKSV AS+VIF
Sbjct: 13  MEEPLLSGTDDCKGGIRTLPFIIASGALEKLASMGLSPSMILYLTEVYGMKSVQASSVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LWTAAT FTPIICAFLADSYFGRF MI AGSI SCLGMFVLWLTA+IP+ARPFCDEISGH
Sbjct: 73  LWTAATYFTPIICAFLADSYFGRFMMIVAGSIFSCLGMFVLWLTAMIPQARPFCDEISGH 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           C+APSTPQLL LYSSY +MSIGSGC+Q+S   FGADQLY+E KSN GILD YFN+ YIS 
Sbjct: 133 CNAPSTPQLLFLYSSYAIMSIGSGCLQSSNFAFGADQLYKENKSNSGILDIYFNLCYISV 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           +VG LVGMSCIVYIQDRMGW +GFGV +ALMLLA++TFLSAS LYLK +PS+SWC GLV 
Sbjct: 193 SVGTLVGMSCIVYIQDRMGWEMGFGVPIALMLLATVTFLSASSLYLKSVPSKSWCAGLVH 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F AYKKR  QIPFV TSEMYHHENGS CTLPSDKLRFLNKACIIKN EEEL S GKAS
Sbjct: 253 VVFAAYKKRRMQIPFVGTSEMYHHENGSSCTLPSDKLRFLNKACIIKNYEEELISDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALIRIIPLWST ILV+A + QS YVLQVASMDRHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIRIIPLWSTGILVSASMSQSVYVLQVASMDRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F  M++VF+IIWI LYDRLILPLAS+YRGKPTRLS KT+MG+ I FC LSLAVSAIVEG 
Sbjct: 373 FIAMLVVFVIIWIILYDRLILPLASRYRGKPTRLSGKTRMGMAIPFCILSLAVSAIVEGY 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAIKEGFSD+PNAVVSMSAFWTLPRYI  G+ EAF  I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIKEGFSDDPNAVVSMSAFWTLPRYILFGIAEAFYAIGQLEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL++CAGNLAASFIMTTVDN SK +GVKSWVSSNINEGHNDYYYWLLFGLLVANFFY
Sbjct: 493 SLLGLNTCAGNLAASFIMTTVDNFSKTIGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 569
           YLACN SYGPSKEESE  SNAED N TVN
Sbjct: 553 YLACNNSYGPSKEESEDISNAEDYNNTVN 581

BLAST of Chy3G062220 vs. NCBI nr
Match: XP_011649736.1 (protein NRT1/ PTR FAMILY 1.2 [Cucumis sativus])

HSP 1 Score: 934 bits (2414), Expect = 0.0
Identity = 473/569 (83.13%), Postives = 510/569 (89.63%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLSDTD  KGG RTLPFIIA+GALEKLAS GLSPSMILYLT+VYGMKS H+SNVIF
Sbjct: 13  MEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHSSNVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LW+AA+NFTPIICAFLADSYFGRFPMIAAGSI S LGMFVLWLTA+IP+ARPFCDEISGH
Sbjct: 73  LWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISGH 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           CDAPST QLLLLYSSY +MS+GSGC+QAS   FGADQLYR+ KSN GILD YFN+ YIS 
Sbjct: 133 CDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISA 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           A+G LVGMSCIVYIQDRMGWG+GFGV VALM LA+ITFLSASPLYLK  PS+SWC GLVQ
Sbjct: 193 ALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQ 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F AYKKRHTQIPFV TSEMYHHEN SPCTLPS+KLRFLNKACII+N++EELT  GKAS
Sbjct: 253 VVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRNSKEELTLDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALIRIIPLWST ILV+A L QSFYVLQVASM+RHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F+ +I+V LIIWI LYDRLILPLASK RGKPTRL AKT+MG+GIL C LSLAVSAIVEG+
Sbjct: 373 FSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCGLSLAVSAIVEGD 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAIKEGFSDNPNAVVSMSAFWTLPRYI  G+ EA + I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL+S  GNLAASFIMTTVDNLSK+VGVKSWVSSNINEGH+DYYYWLL GLL ANF Y
Sbjct: 493 SLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 569
           YLAC+KSYGPS EES GR+NAED   T N
Sbjct: 553 YLACSKSYGPSNEESVGRANAEDYKNTAN 581

BLAST of Chy3G062220 vs. NCBI nr
Match: XP_008445024.1 (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucumis melo])

HSP 1 Score: 926 bits (2394), Expect = 0.0
Identity = 469/569 (82.43%), Postives = 507/569 (89.10%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLSDTD GKGG+RTLPFIIA+GALEKLAS GLSPSMILYLT+VYGMKS  ASNVIF
Sbjct: 13  MEEPLLSDTDDGKGGVRTLPFIIANGALEKLASQGLSPSMILYLTQVYGMKSAQASNVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LW+AA+NFTPIICAFLADSYFGRFPM+AAGSI S LGMFVLWLT +IP+ARP CDEI+GH
Sbjct: 73  LWSAASNFTPIICAFLADSYFGRFPMVAAGSIFSFLGMFVLWLTVMIPQARPLCDEINGH 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           C+APS PQLLLLYSSY +MSIGSGC+QAS   FGADQLYR+ KS  GILD YFNI YIS 
Sbjct: 133 CNAPSIPQLLLLYSSYAIMSIGSGCLQASYLAFGADQLYRKNKSKSGILDNYFNICYISA 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           A+G LVGMSCIVYIQDRMGWG+GFGV V LMLLA+ITFLSAS LYLK MPS+SWC GLVQ
Sbjct: 193 ALGTLVGMSCIVYIQDRMGWGMGFGVPVVLMLLATITFLSASSLYLKSMPSKSWCAGLVQ 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F AYKKRH QI FV TS MYHHENGS C LPSDKLRFLNKACII+N+EEELTS GKAS
Sbjct: 253 VVFAAYKKRHMQIAFVGTSGMYHHENGSLCALPSDKLRFLNKACIIRNSEEELTSDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALI+IIPLWST ILV+A L+QSFYVLQ+ASMDRHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIKIIPLWSTGILVSASLNQSFYVLQLASMDRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F  M++V LIIWITLYDRLILPLASK RGKPTRLSAKT+MG+GIL CTLSLAVSA+VEG 
Sbjct: 373 FGAMLVVSLIIWITLYDRLILPLASKCRGKPTRLSAKTRMGLGILCCTLSLAVSAVVEGY 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAI+EGFSD+PNAVVSMSAFWTLPRYI  GM EAF+ I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIREGFSDDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL+S  GNLAASFIMTTVDN SKAVG KSWVSSNIN+GH+DYYYWLLFGLL AN  Y
Sbjct: 493 SLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYWLLFGLLFANLLY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 569
           +LAC+KSYGPSKEES GRS AED N  VN
Sbjct: 553 FLACSKSYGPSKEESGGRSTAEDYNNAVN 581

BLAST of Chy3G062220 vs. NCBI nr
Match: XP_016899946.1 (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo])

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 471/569 (82.78%), Postives = 506/569 (88.93%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLSDTD GKGGLRTLPFIIA+G  EK+AS GLSPSMILYLTEVYGMKS HASNVIF
Sbjct: 13  MEEPLLSDTDDGKGGLRTLPFIIANGTFEKVASHGLSPSMILYLTEVYGMKSAHASNVIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LW+AATNFTPIICAFLADSYFGRF MIAAGSI S LGMFVLWLTAIIP+ RPFCD+I+G+
Sbjct: 73  LWSAATNFTPIICAFLADSYFGRFSMIAAGSIFSFLGMFVLWLTAIIPQVRPFCDKINGY 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           CDAPSTPQLLLLYSSY +MSIGSGC+QAS   FGADQLY+E KSN GIL+ YFNI YIS 
Sbjct: 133 CDAPSTPQLLLLYSSYAIMSIGSGCLQASHLAFGADQLYKESKSNSGILNSYFNICYISI 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           AVG L+GMSCIVYIQDRMGWGIGFGVSVALMLLA+ITFLSAS LYLK MPS+SWC GLVQ
Sbjct: 193 AVGTLIGMSCIVYIQDRMGWGIGFGVSVALMLLATITFLSASSLYLKSMPSKSWCDGLVQ 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+F  YKKRH QIPFV T EMYHHE GS C LPS+KLRFLNKACII+N EEELTS GKAS
Sbjct: 253 VVFATYKKRHMQIPFVGTFEMYHHETGSLCALPSNKLRFLNKACIIRNFEEELTSDGKAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPWSLCTVEQVENLKALIRIIPLWST IL +  L QSFYVLQVASMDRHLT  FEVPAGS
Sbjct: 313 NPWSLCTVEQVENLKALIRIIPLWSTGILFSVSLSQSFYVLQVASMDRHLTSSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F+ +I+VF+IIWI LY+ LILPLASK RGKPT LS KT+MG+GIL CTLSLAVSAIVEG+
Sbjct: 373 FSVLIVVFVIIWIILYNCLILPLASKCRGKPTHLSGKTRMGLGILCCTLSLAVSAIVEGD 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAIKEGFS++PNAVVSMSAFWTLPRYI  GM EAF+ I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIKEGFSNDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL+S  GNLAASFIMTTVDN SKAVG KSWVSSNIN+GH+DYYYWLLFGLL ANF Y
Sbjct: 493 SLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYWLLFGLLFANFLY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKTVN 569
           +LAC+KSYGPSKEES GRS AED N TVN
Sbjct: 553 FLACSKSYGPSKEESAGRSTAEDYNNTVN 581

BLAST of Chy3G062220 vs. NCBI nr
Match: XP_038885652.1 (protein NRT1/ PTR FAMILY 1.2 isoform X4 [Benincasa hispida])

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 454/567 (80.07%), Postives = 494/567 (87.13%), Query Frame = 0

Query: 1   MEEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIF 60
           MEEPLLS  D  KGGLRTLPFIIA+GALEKLAS GLSPSMILYLT VYGMKS +ASN+IF
Sbjct: 13  MEEPLLSTADH-KGGLRTLPFIIANGALEKLASQGLSPSMILYLTMVYGMKSAYASNIIF 72

Query: 61  LWTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGH 120
           LW+AATNFTPIICAFLADSYFGRFPMIAAGSI S LGMFVLWLTA+IP+ARPFCDEISG 
Sbjct: 73  LWSAATNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISGR 132

Query: 121 CDAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYIST 180
           CDAPS  QL LLYSSY +MSIGSGC+Q+S   FGADQLYR  KSN GILD YFN+ YIS+
Sbjct: 133 CDAPSRAQLSLLYSSYAIMSIGSGCLQSSYLAFGADQLYRTSKSNSGILDSYFNLCYISS 192

Query: 181 AVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQ 240
           AVG+LVGMS +VYIQDRMGWG+GFGV VALMLL+ ITF SASPLYLK MPSRSWC G+VQ
Sbjct: 193 AVGSLVGMSGMVYIQDRMGWGMGFGVPVALMLLSMITFFSASPLYLKSMPSRSWCAGIVQ 252

Query: 241 VIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKAS 300
           V+  A KKRH Q+P V   E YHHENGSPC LPSDKLRF NKACII+N+EEELTS  +AS
Sbjct: 253 VVVAACKKRHMQVPSVGIPETYHHENGSPCALPSDKLRFFNKACIIRNSEEELTSDRRAS 312

Query: 301 NPWSLCTVEQVENLKALIRIIPLWSTEILVAACLHQSFYVLQVASMDRHLTPGFEVPAGS 360
           NPW+LCTVEQVE+LKAL+RIIPLWST ILV+A L QSFY LQVASMDRHLTP FEVPAGS
Sbjct: 313 NPWTLCTVEQVEDLKALVRIIPLWSTGILVSAALSQSFYTLQVASMDRHLTPSFEVPAGS 372

Query: 361 FTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGN 420
           F  M++V LIIWITLY+R+ILPLASK RGKPTRLS KT+MGIGILF TLSLAVSAIVE N
Sbjct: 373 FGAMLVVSLIIWITLYNRVILPLASKCRGKPTRLSGKTRMGIGILFSTLSLAVSAIVESN 432

Query: 421 RRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAI 480
           RRALAIKEGFSD+PNAVV+MSAFWTLPRYI  GM E F+ I Q+EFFY ELPKAMSSVA 
Sbjct: 433 RRALAIKEGFSDDPNAVVNMSAFWTLPRYILFGMGEGFNAIGQIEFFYNELPKAMSSVAT 492

Query: 481 SLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFY 540
           SLLGL+   GNLAASFIMTTVDN SKA  VKSWVSSNIN+GH+DYYYWLLFGLL ANF Y
Sbjct: 493 SLLGLNMSVGNLAASFIMTTVDNFSKAAEVKSWVSSNINKGHSDYYYWLLFGLLFANFLY 552

Query: 541 YLACNKSYGPSKEESEGRSNAEDSNKT 567
           +LAC+KSYGPSKEE+ G SNAED N T
Sbjct: 553 FLACSKSYGPSKEEAGGGSNAEDHNNT 578

BLAST of Chy3G062220 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.5e-144
Identity = 269/558 (48.21%), Postives = 373/558 (66.85%), Query Frame = 0

Query: 13  KGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPII 72
           KGG+ T+PFIIA+ A EK+AS GL P+MI+YL   Y       +NV+F+W+AA+NFTP++
Sbjct: 22  KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLL 81

Query: 73  CAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPF-CDEIS--GHCDAPSTPQL 132
            AFL+DSY GRF  I+  S+ S LGM +LWLTA++P+ +P  CD  +   HC + +  QL
Sbjct: 82  GAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQL 141

Query: 133 LLLYSSYIVMSIGSGCVQASCPVFGADQL-YREKKSNIGILDRYFNIFYISTAVGALVGM 192
            LLYS++ ++SIGSG ++     FGADQL  +E   N  +L+ +F  +Y S+AV  L+  
Sbjct: 142 ALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAF 201

Query: 193 SCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKK 252
           + IVYIQ+ +GW IGFGV   LML+A++ F+ ASPLY+    ++S   GL Q I  AYKK
Sbjct: 202 TGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKK 261

Query: 253 RHTQIP-FVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKASNPWSLCT 312
           R   +P   D+ + Y+H   S    PS KLRFLNKAC+I N EEE+ S G A NPW LCT
Sbjct: 262 RKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCT 321

Query: 313 VEQVENLKALIRIIPLWSTEILVAACLHQ-SFYVLQVASMDRHLT---PGFEVPAGSFTG 372
            ++VE LKALI++IP+WST I+++    Q SF +LQ  SMDR L+     F+VPAGSF  
Sbjct: 322 TDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGM 381

Query: 373 MILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRRA 432
             ++ L +W+ LYDR ++PLASK RG+P RLS K +MG+G+    L++A+SA+VE  RR 
Sbjct: 382 FTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRK 441

Query: 433 LAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISLL 492
            AI +G+++N NAVV +SA W +P+Y+  G+ EA + I Q EFFY E PK+MSS+A SL 
Sbjct: 442 KAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLF 501

Query: 493 GLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYLA 552
           GL     +L AS ++  V+ L+   G +SWVS NIN+GH +YYYW+L  +   N  YY+ 
Sbjct: 502 GLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVI 561

Query: 553 CNKSYGPSKEE-SEGRSN 561
           C+ SYGP  ++   GR N
Sbjct: 562 CSWSYGPLVDQVRNGRVN 579

BLAST of Chy3G062220 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 497.3 bits (1279), Expect = 1.7e-140
Identity = 266/549 (48.45%), Postives = 362/549 (65.94%), Query Frame = 0

Query: 13  KGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPII 72
           KGGL T+PFIIA+   EK+AS GL  +MILYL   Y +  V    V+F+W AATNF P++
Sbjct: 24  KGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLV 83

Query: 73  CAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPF-CDEISG-HCDAPSTPQLL 132
            AFL+DSY GRF  I   S+ S LGM VLWLTA++P+ +P  C   +G +C + ++ QL 
Sbjct: 84  GAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLA 143

Query: 133 LLYSSYIVMSIGSGCVQASCPVFGADQL-YREKKSNIGILDRYFNIFYISTAVGALVGMS 192
           LLY+++ ++SIGSG ++     FGADQL  +E   N  +L+ +F  +Y S++V  L+  +
Sbjct: 144 LLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFT 203

Query: 193 CIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKKR 252
            IVYIQD +GW IGFG+   LMLLA   F+ ASPLY+K   S+S   GL QV+  AY KR
Sbjct: 204 VIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKR 263

Query: 253 HTQIP-FVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGKASNPWSLCTV 312
           +  +P   D+ + Y+    S    PSDKLRFLNKAC I N +E+L S G A N W LCT 
Sbjct: 264 NLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTT 323

Query: 313 EQVENLKALIRIIPLWSTEILVAACLHQ-SFYVLQVASMDRHLTPG--FEVPAGSFTGMI 372
           +QVE LKAL+++IP+WST I+++  + Q SF +LQ  SMDR L+    F++PAGSF    
Sbjct: 324 DQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFT 383

Query: 373 LVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRRALA 432
           ++ LI W+ LYDR ILPLASK RG+P R++ K +MG+G+    L++AVSA VE  RR  A
Sbjct: 384 IIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTA 443

Query: 433 IKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISLLGL 492
           I +G +++ N+ VS+SA W +P+Y+  G+ EA + I Q EFFY E PK+MSS+A SL GL
Sbjct: 444 ISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGL 503

Query: 493 SSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYLACN 552
                N+ AS I+  V N SK  G  SW+  NIN+GH DYYYW+L  L   N  YY+ C+
Sbjct: 504 GMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCS 563

Query: 553 KSYGPSKEE 555
            SYGP+ ++
Sbjct: 564 WSYGPTVDQ 571

BLAST of Chy3G062220 vs. TAIR 10
Match: AT5G11570.1 (Major facilitator superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 8.4e-100
Identity = 225/555 (40.54%), Postives = 306/555 (55.14%), Query Frame = 0

Query: 2   EEPLLSDTDGGKGGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFL 61
           +E LL      K G+ T+PFI+AS ALEKLA  GL P+MIL+LT  YGM +  A+N++FL
Sbjct: 3   QEALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILFL 62

Query: 62  WTAATNFTPIICAFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGHC 121
           W+AATNF P++ AF+ADSY GRFP+I  GS IS  GM +LWLT II   RP CD+++  C
Sbjct: 63  WSAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNVC 122

Query: 122 DAPSTPQLLLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNI--GILDRYFNIFYIS 181
              +  + +LLYS + + +IG+G V++SC  F ADQL   + S +    L+  FN +Y S
Sbjct: 123 QPTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYFS 182

Query: 182 TAVGALVGMSCIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLM-PSRSWCVGL 241
             V   +  S +V++Q   GW IGFGVSVA M L+   F +ASP Y++   P+R+     
Sbjct: 183 VMVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYVRFQKPTRN----- 242

Query: 242 VQVIFVAYKKRHTQIPFVDTSEMYHHENGSPCTLPSDKLRFLNKACIIKNAEEELTSYGK 301
                                                                       
Sbjct: 243 ------------------------------------------------------------ 302

Query: 302 ASNPWSLCTVEQVENLKALIRIIPLWSTEI---LVAACLHQSFYVLQVASMDRH-LTPGF 361
           + NPW LC V+QVE+LK+LI +IP+WST I   LV AC   SF VLQ  +MDRH    GF
Sbjct: 303 SRNPWKLCRVQQVEDLKSLINVIPIWSTGIILSLVTAC-QVSFIVLQAKTMDRHTFIQGF 362

Query: 362 EVPAGSFTGMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVS 421
           E+P GS+   +++  ++++ LYD +I+PL S    +P RL    +M  G +   L ++  
Sbjct: 363 EIPPGSYGIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISAL 422

Query: 422 AIVEGNRRALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKA 481
           A  E  RR  A  E       +   +SA W LP  I  G+ EA + IAQ EFFY ELPK 
Sbjct: 423 AATEYARRKTARDE-------SGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKT 477

Query: 482 MSSVAISLLGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLL 541
           MSSVA +L  L+  A +L +S+I+T VD         SW++ NI+EGH DYYYWLL GL 
Sbjct: 483 MSSVATTLSSLNMAAASLISSWIITIVD----VTTYGSWITENIDEGHLDYYYWLLVGLS 477

Query: 542 VANFFYYLACNKSYG 550
           + N  Y++ C KSYG
Sbjct: 543 LLNVLYFVWCKKSYG 477

BLAST of Chy3G062220 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 329.3 bits (843), Expect = 6.0e-90
Identity = 194/569 (34.09%), Postives = 317/569 (55.71%), Query Frame = 0

Query: 14  GGLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPIIC 73
           GG R + FI+ +  LE+L S+GL  + ++YLT+V+ ++ V A+NVI +W+  TN TP++ 
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 74  AFLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFC----DEISGHCDAPSTPQL 133
           A+++D+Y GRF  IA  S  + LG+  + LTA  P+  P      D +S  C  P+  Q+
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPNKLQI 172

Query: 134 LLLYSSYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYISTAVGALVGMS 193
            +L      +S+GSG ++     FG DQ  +  +  +  +  +FN +Y++  V  ++  +
Sbjct: 173 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 232

Query: 194 CIVYIQDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKKR 253
            +VYIQD++ W IGF +   LM LA + F +    Y+ + P  S   G+ QVI  A KKR
Sbjct: 233 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 292

Query: 254 HTQIPFVDTSEMYHHENGSPCTL-----PSDKLRFLNKACIIKNAEEELTSYGKASNPWS 313
             ++P  D   + +++     ++      S++ R L+KA ++   E +LT  G  ++ W 
Sbjct: 293 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 314 LCTVEQVENLKALIRIIPLWSTEI--LVAACLHQSFYVLQVASMDRHLTPGFEVPAGSFT 373
           LC+V++VE +K LIRI+P+WS  I  L A     +F V Q   MDR+L P FE+PAGS +
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 374 GMILVFLIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRR 433
            + L+ + I++  YDR+ +P   +  G  + ++   ++G GI+F   S+ V+ IVE  RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 434 ALAIKEGFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISL 493
             +I  G   +P  +  MS FW  P+ I +G+ EAF++I Q+EFF  + P+ M S+A SL
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 494 LGLSSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYL 553
             LS    +  +SF++T V   S       W++ N+N G  DY+Y+L+  L V N  Y+ 
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFW 592

Query: 554 ACNKSY----GPSKEESEGRSNAEDSNKT 568
            C + Y    G   E+ E   +++D   T
Sbjct: 593 YCARGYRYKVGLPIEDFEEDKSSDDVEMT 614

BLAST of Chy3G062220 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 2.1e-87
Identity = 187/565 (33.10%), Postives = 312/565 (55.22%), Query Frame = 0

Query: 15  GLRTLPFIIASGALEKLASVGLSPSMILYLTEVYGMKSVHASNVIFLWTAATNFTPIICA 74
           G + +PFII +   EKL  +G   ++++YLT V+ +KS  A+ +I  ++   NF   I A
Sbjct: 64  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAA 123

Query: 75  FLADSYFGRFPMIAAGSIISCLGMFVLWLTAIIPRARPFCDEISGHCDAPSTPQLLLLYS 134
           FL D+YFGR+  ++   I   LG FV+ LTA IP   P        C+ PS  Q+L L  
Sbjct: 124 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKISCEGPSVGQILFLLM 183

Query: 135 SYIVMSIGSGCVQASCPVFGADQLYREKKSNIGILDRYFNIFYISTAVGALVGMSCIVYI 194
               + +G+G ++     FGADQ   + +S    ++ +FN ++ +     ++ ++ +VYI
Sbjct: 184 GLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYI 243

Query: 195 QDRMGWGIGFGVSVALMLLASITFLSASPLYLKLMPSRSWCVGLVQVIFVAYKKRHTQIP 254
           Q  + W IG  + VALM LA + F +   LY+K+  S S   G+ +VI  A KKR  +  
Sbjct: 244 QSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPV 303

Query: 255 FVDTSEMYHH--ENGSPCTLP-SDKLRFLNKACIIKNAEEELTSYGKASNPWSLCTVEQV 314
                 +Y+H   N +  TL  +D+ RFL+KA I+   EE+L S G AS+PW LCT++QV
Sbjct: 304 KQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIM-TPEEKLNSDGTASDPWKLCTLQQV 363

Query: 315 ENLKALIRIIPLW--STEILVAACLHQSFYVLQVASMDRHL-TPGFEVPAGSFTGMILVF 374
           E +K ++R+IP+W  ST   +A  +  ++ V Q    DR L + GF +PA ++   ++  
Sbjct: 364 EEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTG 423

Query: 375 LIIWITLYDRLILPLASKYRGKPTRLSAKTKMGIGILFCTLSLAVSAIVEGNRRALAIKE 434
           + ++I  YDR+++P   +  G  T +S   ++G G  F  +SL VS  +E  RR  A+ +
Sbjct: 424 MTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTK 483

Query: 435 ---GFSDNPNAVVSMSAFWTLPRYIFLGMEEAFSMIAQVEFFYFELPKAMSSVAISLLGL 494
              G +     + SMSA W +P+    G+ EAF+ I Q+EF+Y + P+ M S A S+  +
Sbjct: 484 PTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYV 543

Query: 495 SSCAGNLAASFIMTTVDNLSKAVGVKSWVSSNINEGHNDYYYWLLFGLLVANFFYYLACN 554
            +   +  ASF+++TV   +      +W++ ++N+   DY+Y++L GL+V N  Y+L   
Sbjct: 544 GAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTGLMVVNMAYFLLMA 603

Query: 555 KSY----GPSKEESEGRSNAEDSNK 567
           + Y    G  ++ +E  +N E++ +
Sbjct: 604 RWYRYKGGNDEDITEIETNEEETKQ 627