Chy3G061450 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G061450
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	gibberellin 3-beta-dioxygenase 1-like
Location	chrH03: 14858763 .. 14859871 (-)
RNA-Seq Expression	Chy3G061450
Synteny	Chy3G061450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGATCAAGAGATTCCTCCCCCAAGCCAACTTGTTCCTTTGGACTTCACCTCAGTCCAAACTGTCCCTGAATCTCACTTGTGGCCAAACGCCAAGGAGTTATCGGCTGCGATCAAGTTGGACAAAAGGGTTTCGCTCCCGTTAATCGACCTTGTCAGCGATGGTGCGTCGGAGCTCCTAGGGAGAGCCTGTGAGGAATGGGGAATGTTTCAGTTGATTAATCACGGTGTCTCGAAAACCCTAATCAAGGAAGCAGAGGAGGAGACTCGACGTCTTTTCACTCTCCCGGCAACACAAAAGATGAAGACACTACGATCCCCTGGAAGCCTCACCGCCACCGGCTACGGCATGGCTGGAATATCGAAGTATCATCCGAAATTAATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCTATTAATGAGGCTAAGAAGCTTTGGCCAAATGACTATAAACGGTTTTGGTAAATCCATGCAATATACGTACACAATTTAGGATTCATTTCCGTAACGTATATGCATATAAATTAAATTTTGTTAAGTAACTTTAATTTCATCATATAATTAATTGGAGCTAAAATTATCATAATCTATAGGTTCAATCATTAATTTTAATTTTAATTTGGTGTTTACTCCAGCAATTACATATCATCACGTTTTACTCATTTTTTTTTCTATTTATATTCAGAAAAAAAAATTACGTAGTATTTAAAAATGAAATATTAGAAGAATACATTTTTTTTAGAAAAAATGGAATTAAGTAAAAATAAATATAGTTTACTAACAAAGAGTCTAAAAATTATGAAATTTAAGGCTTTAAATTGATACAATAATTTATAATAATTCTTGAACTTTAGAGAATATAGAAATGGAGTTTTGACTGTTTACCAAATTTTGGTAGAAATTTAGTGGTTAGTTTTAAGATTTAGAAAGTATTAAGAACTAATTTTGGACAATACACAAAATGTTGTACCATTTTAAAAACAAAATAGATTATTGAAATAATTCGAGACCAAAAATTAAAAAGATTTGCTTAAATTGTTTTATGTTTTAAATCTTCCATTTGTGTTTTGAGATTATAGAAATATGATGGTTTTATGATCTAA

mRNA sequence

ATGGCAGATCAAGAGATTCCTCCCCCAAGCCAACTTGTTCCTTTGGACTTCACCTCAGTCCAAACTGTCCCTGAATCTCACTTGTGGCCAAACGCCAAGGAGTTATCGGCTGCGATCAAGTTGGACAAAAGGGTTTCGCTCCCGTTAATCGACCTTGTCAGCGATGGTGCGTCGGAGCTCCTAGGGAGAGCCTGTGAGGAATGGGGAATGTTTCAGTTGATTAATCACGGTGTCTCGAAAACCCTAATCAAGGAAGCAGAGGAGGAGACTCGACGTCTTTTCACTCTCCCGGCAACACAAAAGATGAAGACACTACGATCCCCTGGAAGCCTCACCGCCACCGGCTACGGCATGGCTGGAATATCGAAGTATCATCCGAAATTAATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCTATTAATGAGGCTAAGAAGCTTTGGCCAAATGACTATAAACGAAAAAAAAATTACGTAAAATATGATGGTTTTATGATCTAA

Coding sequence (CDS)

ATGGCAGATCAAGAGATTCCTCCCCCAAGCCAACTTGTTCCTTTGGACTTCACCTCAGTCCAAACTGTCCCTGAATCTCACTTGTGGCCAAACGCCAAGGAGTTATCGGCTGCGATCAAGTTGGACAAAAGGGTTTCGCTCCCGTTAATCGACCTTGTCAGCGATGGTGCGTCGGAGCTCCTAGGGAGAGCCTGTGAGGAATGGGGAATGTTTCAGTTGATTAATCACGGTGTCTCGAAAACCCTAATCAAGGAAGCAGAGGAGGAGACTCGACGTCTTTTCACTCTCCCGGCAACACAAAAGATGAAGACACTACGATCCCCTGGAAGCCTCACCGCCACCGGCTACGGCATGGCTGGAATATCGAAGTATCATCCGAAATTAATGTGGCATGAAGGATTCACCATCTTGGGTTCTCCTATTAATGAGGCTAAGAAGCTTTGGCCAAATGACTATAAACGAAAAAAAAATTACGTAAAATATGATGGTTTTATGATCTAA

Protein sequence

MADQEIPPPSQLVPLDFTSVQTVPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKRKKNYVKYDGFMI*

Homology

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BLAST of Chy3G061450 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.0e-30
Identity = 66/155 (42.58%), Postives = 103/155 (66.45%), Query Frame = 0


Query: 16  DFTSVQTVPESHLWPNAKEL----SAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMF 75
           DF S+Q +PES+ W +  +     S  I  +   ++P+IDL    AS+L+G AC+ WG++
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGVY 80

Query: 76  QLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWH 135
           Q++NHG+  +L+++ +   + LF+LP+ QK K  RSP  +  +GYG+A IS + PKLMW+
Sbjct: 81  QVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGV--SGYGIARISSFFPKLMWY 140

Query: 136 EGFTILGSPINEAKKLWPNDYKRKKN-YVKYDGFM 166
           EGFTI+GSP++  ++LWP DY R  +  V+YD  M
Sbjct: 141 EGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETM 173

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BLAST of Chy3G061450 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.0e-26
Identity = 59/145 (40.69%), Postives = 93/145 (64.14%), Query Frame = 0


Query: 11  QLVPLDFTSVQTVPESHLW--PNAKELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEW 70
           +++PLDFT+ +T+P+SH+W  P  +  S  I        P+I L +     LL +ACEEW
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKL 130
           G+F + +HGVS +L+   + + +RLF+LP  +K+  +RSP    +TGYG+  IS ++ KL
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDE--STGYGVVRISMFYDKL 136

Query: 131 MWHEGFTILGSPI-NEAKKLWPNDY 153
           MW EGF+++GS +   A  LWP+D+
Sbjct: 137 MWSEGFSVMGSSLRRHATLLWPDDH 151

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BLAST of Chy3G061450 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 1.6e-24
Identity = 60/147 (40.82%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 9   PSQLVPLDFTSVQTVPESHLWPNAKEL---SAAIKLDKRVSLPLIDLVSDGASELLGRAC 68
           P   +P DFTS++ +P+S+ W    +L   +A        ++PLIDL    A+  +G AC
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHAC 76

Query: 69  EEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYH 128
             WG FQ+ NHGV   L+++ E  T  LF LP  +K+K+ RS   +  +GYG+A I+ + 
Sbjct: 77  RTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGV--SGYGVARIASFF 136

Query: 129 PKLMWHEGFTILGSPINEAKKLWPNDY 153
            K MW EGFTI GSP+N+ +KLWP  +
Sbjct: 137 NKQMWSEGFTITGSPLNDFRKLWPQHH 160

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BLAST of Chy3G061450 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-23
Identity = 59/140 (42.14%), Postives = 85/140 (60.71%), Query Frame = 0


Query: 14  PLDFTSVQTVPESHLW-PNAKELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMFQ 73
           P DF S   +P+S+ W P    L +A   D+  +LPLIDL     + L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 133
           + NHGV   L+ + E  T  LF LP  +K+K  RS   +  +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGV--SGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPINEAKKLWPNDY 153
           GFT++GSP+++ +KLWP+ +
Sbjct: 141 GFTVIGSPLHDFRKLWPSHH 153

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BLAST of Chy3G061450 vs. ExPASy Swiss-Prot
Match: Q9FU53 (Gibberellin 3-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA3OX2 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 9.9e-22
Identity = 55/150 (36.67%), Postives = 85/150 (56.67%), Query Frame = 0


Query: 6   IPPPSQL---VPLDFTSVQTVPESHLWPNAKE--LSAAIKLDKRVSLPLIDLVSDGASEL 65
           +P PS L   +  DF + + VPE+H WP   +  +          ++P++D+ +  A+  
Sbjct: 1   MPTPSHLKNPLCFDFRAARRVPETHAWPGLDDHPVVDGGGGGGEDAVPVVDVGAGDAAAR 60

Query: 66  LGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 125
           + RA E+WG F L+ HGV   L+   EE   R+F+LPA++KM+ +R PG     GYG   
Sbjct: 61  VARAAEQWGAFLLVGHGVPAALLSRVEERVARVFSLPASEKMRAVRGPGE--PCGYGSPP 120

Query: 126 ISKYHPKLMWHEGFTILGSPI-NEAKKLWP 150
           IS +  KLMW EG+T   S + +E ++LWP
Sbjct: 121 ISSFFSKLMWSEGYTFSPSSLRSELRRLWP 148

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BLAST of Chy3G061450 vs. ExPASy TrEMBL
Match: S0BE47 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.2e-82
Identity = 151/154 (98.05%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQEIPPPSQLVPLDFTSVQTVPESHLWPNA ELSAAIKLDKRVSLPLIDLVSDGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACEEWGMFQLINHG+SKTLI+EAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 155
           ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154

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BLAST of Chy3G061450 vs. ExPASy TrEMBL
Match: A0A0A0LLT9 (DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379310 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 5.2e-82
Identity = 151/154 (98.05%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQEIPPPSQLVPLDFTSVQTVPESHLWPNA ELSAAIKLDKRVSLPLIDLVSDGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACEEWGMFQLINHG+SKTLI+EAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 155
           ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154

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BLAST of Chy3G061450 vs. ExPASy TrEMBL
Match: A0A1S3BBH9 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE=3 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 2.9e-77
Identity = 144/155 (92.90%), Postives = 150/155 (96.77%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN KELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHGVS+TLI+EAEEETRRLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 155
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKR
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKR 155

[Close]

BLAST of Chy3G061450 vs. ExPASy TrEMBL
Match: A0A5A7VHL7 (Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002900 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.1e-76
Identity = 143/155 (92.26%), Postives = 150/155 (96.77%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN KELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNIKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHGVS+TLI+EAEEET+RLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETQRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 155
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKR
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKR 155

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BLAST of Chy3G061450 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.0e-69
Identity = 128/154 (83.12%), Postives = 142/154 (92.21%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQLINHGV KTLI EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRT-DSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 155
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKR
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKR 153

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BLAST of Chy3G061450 vs. NCBI nr
Match: NP_001275523.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetical protein Csa_021993 [Cucumis sativus] >CCG14217.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 310 bits (794), Expect = 2.50e-103
Identity = 151/154 (98.05%), Postives = 153/154 (99.35%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQEIPPPSQLVPLDFTSVQTVPESHLWPNA ELSAAIKLDKRVSLPLIDLVSDGASEL
Sbjct: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASEL 60

Query: 61  LGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACEEWGMFQLINHG+SKTLI+EAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG
Sbjct: 61  LGRACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154
           ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR
Sbjct: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154

[Close]

BLAST of Chy3G061450 vs. NCBI nr
Match: KAA0064969.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 292 bits (748), Expect = 3.68e-99
Identity = 143/155 (92.26%), Postives = 150/155 (96.77%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN KELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNIKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHGVS+TLI+EAEEET+RLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETQRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKR
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKR 155

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BLAST of Chy3G061450 vs. NCBI nr
Match: XP_008445138.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 294 bits (753), Expect = 4.31e-97
Identity = 144/155 (92.90%), Postives = 150/155 (96.77%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQT-VPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASE 60
           MADQEIPPPSQLVPL+F SVQT VPESHLWPN KELSAA+KLDKRVSLPLIDLV+D ASE
Sbjct: 1   MADQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASE 60

Query: 61  LLGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMA 120
           LLGRACEEWGMFQLINHGVS+TLI+EAEEETRRLFTLPA QKMKTLRSPGSLTATGYGMA
Sbjct: 61  LLGRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMA 120

Query: 121 GISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154
           GISKYHPKLMWHEGFTI+GSPINEAKKLWPNDYKR
Sbjct: 121 GISKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKR 155

[Close]

BLAST of Chy3G061450 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 268 bits (684), Expect = 1.17e-86
Identity = 128/154 (83.12%), Postives = 142/154 (92.21%), Query Frame = 0


Query: 1   MADQEIPPPSQLVPLDFTSVQTVPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASEL 60
           MADQE+PPPSQLVPLDF S+QTVPESHLWPN+ ELS AIKLD+R+SLP+ID + DGASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  LGRACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAG 120
           LGRACE+WGMFQLINHGV KTLI EAEEETRRLF+LPA QKMKTLR+  SLTATGYGMAG
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTD-SLTATGYGMAG 120

Query: 121 ISKYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154
           ISKYH KLMWHEGFT+ GSP+++AKKLWPNDYKR
Sbjct: 121 ISKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKR 153

[Close]

BLAST of Chy3G061450 vs. NCBI nr
Match: XP_038885582.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 265 bits (678), Expect = 9.75e-86
Identity = 124/152 (81.58%), Postives = 141/152 (92.76%), Query Frame = 0


Query: 3   DQEIPPPSQLVPLDFTSVQTVPESHLWPNAKELSAAIKLDKRVSLPLIDLVSDGASELLG 62
           DQE+PPPSQ VPLDF+S+QTVPESHLWP++ ELS A++LD+RVSLP+IDL+ +GASELLG
Sbjct: 5   DQELPPPSQFVPLDFSSIQTVPESHLWPDSNELSLAVELDERVSLPIIDLLDNGASELLG 64

Query: 63  RACEEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGIS 122
           RACEEWGMFQLINHGV KTLI EAEEETRRLFTLPA QKMK L+SP S+T  GYGMAGIS
Sbjct: 65  RACEEWGMFQLINHGVPKTLIDEAEEETRRLFTLPARQKMKALQSPSSIT--GYGMAGIS 124

Query: 123 KYHPKLMWHEGFTILGSPINEAKKLWPNDYKR 154
           KYHPKLMWHEGFT+LGSP+++AKKLWPNDYKR
Sbjct: 125 KYHPKLMWHEGFTVLGSPVDDAKKLWPNDYKR 154

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BLAST of Chy3G061450 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 119.8 bits (299), Expect = 2.1e-27
Identity = 59/145 (40.69%), Postives = 93/145 (64.14%), Query Frame = 0


Query: 11  QLVPLDFTSVQTVPESHLW--PNAKELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEW 70
           +++PLDFT+ +T+P+SH+W  P  +  S  I        P+I L +     LL +ACEEW
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTSGPI--------PVISLSNPEEHGLLRQACEEW 76

Query: 71  GMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKL 130
           G+F + +HGVS +L+   + + +RLF+LP  +K+  +RSP    +TGYG+  IS ++ KL
Sbjct: 77  GVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDE--STGYGVVRISMFYDKL 136

Query: 131 MWHEGFTILGSPI-NEAKKLWPNDY 153
           MW EGF+++GS +   A  LWP+D+
Sbjct: 137 MWSEGFSVMGSSLRRHATLLWPDDH 151

[Close]

BLAST of Chy3G061450 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 114.0 bits (284), Expect = 1.2e-25
Identity = 60/147 (40.82%), Postives = 88/147 (59.86%), Query Frame = 0


Query: 9   PSQLVPLDFTSVQTVPESHLWPNAKEL---SAAIKLDKRVSLPLIDLVSDGASELLGRAC 68
           P   +P DFTS++ +P+S+ W    +L   +A        ++PLIDL    A+  +G AC
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHAC 76

Query: 69  EEWGMFQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYH 128
             WG FQ+ NHGV   L+++ E  T  LF LP  +K+K+ RS   +  +GYG+A I+ + 
Sbjct: 77  RTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGV--SGYGVARIASFF 136

Query: 129 PKLMWHEGFTILGSPINEAKKLWPNDY 153
            K MW EGFTI GSP+N+ +KLWP  +
Sbjct: 137 NKQMWSEGFTITGSPLNDFRKLWPQHH 160

[Close]

BLAST of Chy3G061450 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 110.9 bits (276), Expect = 9.8e-25
Identity = 59/140 (42.14%), Postives = 85/140 (60.71%), Query Frame = 0


Query: 14  PLDFTSVQTVPESHLW-PNAKELSAAIKLDKRVSLPLIDLVSDGASELLGRACEEWGMFQ 73
           P DF S   +P+S+ W P    L +A   D+  +LPLIDL     + L+G AC  WG FQ
Sbjct: 21  PPDFKS---LPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTTWGAFQ 80

Query: 74  LINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 133
           + NHGV   L+ + E  T  LF LP  +K+K  RS   +  +GYG+A I+ +  K MW E
Sbjct: 81  ITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGV--SGYGVARIASFFNKKMWSE 140

Query: 134 GFTILGSPINEAKKLWPNDY 153
           GFT++GSP+++ +KLWP+ +
Sbjct: 141 GFTVIGSPLHDFRKLWPSHH 153

[Close]

BLAST of Chy3G061450 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 94.4 bits (233), Expect = 9.5e-20
Identity = 52/143 (36.36%), Postives = 81/143 (56.64%), Query Frame = 0


Query: 19  SVQTVPESHLW-----PNAKELSAAIKLDK-RVSLPLIDLVSDGASELLGRACEEWGMFQ 78
           S+QT+PES  W      +    S+A+  D    S+P+IDL +   + L+G A + WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMWHE 138
           + NHG+S+ L+ + E  ++ LF +P+ +K++   S   +  +GYG   IS +  K MW E
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGV--SGYGEPRISPFFEKKMWSE 134

Query: 139 GFTIL-GSPINEAKKLWPNDYKR 155
           GFTI   S  N    LWP+D+ +
Sbjct: 135 GFTIADDSYRNHFNTLWPHDHTK 155

[Close]

BLAST of Chy3G061450 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.0e-13
Identity = 43/140 (30.71%), Postives = 69/140 (49.29%), Query Frame = 0


Query: 15  LDFTSVQTVPESHLWPNAKEL-SAAIKLDKRVSLPLID---LVSDGASELLGRACEEWGM 74
           L  T ++ +P+ ++ P  + L +  +K D   S+P+ID   L     S+ +  A EEWG 
Sbjct: 29  LSETGIKVLPDQYIQPFEERLINFHVKEDSDESIPVIDISNLDEKSVSKAVCDAAEEWGF 88

Query: 75  FQLINHGVSKTLIKEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGISKYHPKLMW 134
           FQ+INHGVS  +++  +  T R F LP  +K K  R     T   +G +        L W
Sbjct: 89  FQVINHGVSMEVLENMKTATHRFFGLPVEEKRKFSREKSLSTNVRFGTSFSPHAEKALEW 148

Query: 135 HEGFTILGSPINEAKKLWPN 151
            +  ++      EA +LWP+
Sbjct: 149 KDYLSLFFVSEAEASQLWPD 168

The following BLAST results are available for this feature:

BLAST of Chy3G061450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	2.0e-30	42.58	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q9SVS8	3.0e-26	40.69	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]
Q39103	1.6e-24	40.82	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9ZT84	1.4e-23	42.14	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q9FU53	9.9e-22	36.67	Gibberellin 3-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA3...	[more]

BLAST of Chy3G061450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
S0BE47	5.2e-82	98.05	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox4 PE=2 SV=1	[more]
A0A0A0LLT9	5.2e-82	98.05	DIOX_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379310 PE=4...	[more]
A0A1S3BBH9	2.9e-77	92.90	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488263 PE...	[more]
A0A5A7VHL7	1.1e-76	92.26	Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
Q9LM06	3.0e-69	83.12	Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE...	[more]

BLAST of Chy3G061450 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001275523.1	2.50e-103	98.05	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetic...	[more]
KAA0064969.1	3.68e-99	92.26	gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa]	[more]
XP_008445138.1	4.31e-97	92.90	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
AAF80661.1	1.17e-86	83.12	putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris]	[more]
XP_038885582.1	9.75e-86	81.58	gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida]	[more]

BLAST of Chy3G061450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21690.1	2.1e-27	40.69	gibberellin 3-oxidase 3	[more]
AT1G15550.1	1.2e-25	40.82	gibberellin 3-oxidase 1	[more]
AT1G80340.1	9.8e-25	42.14	gibberellin 3-oxidase 2	[more]
AT1G80330.1	9.5e-20	36.36	gibberellin 3-oxidase 4	[more]
AT1G55290.1	1.0e-13	30.71	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 11..162 e-value: 2.4E-25 score: 91.8
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 48..149 e-value: 5.2E-20 score: 72.4
None	No IPR available	PANTHER	PTHR47990:SF105	GIBBERELLIN 2-BETA-DIOXYGENASE	coord: 9..156
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 9..156
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 14..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G061450.1	Chy3G061450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding