Chy3G061220 (gene) Cucumber (hystrix) v1

Overview
NameChy3G061220
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionacyl-protein thioesterase 2-like
LocationchrH03: 14685817 .. 14690450 (-)
RNA-Seq ExpressionChy3G061220
SyntenyChy3G061220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTATAACAGCTCTACTGTGGGCTCTGGTTTGTTTATTTTCTGTTCATTTTGTTTTATTGGTTTACCGTCCTTAAGAATAAATGGCTTAGATTTCATTTATGCATCTGGGGTTTTTCCCCCTCAACTTTTTGCTCCATTATGTCAGGTAGTAGAACTGGGAGGATGACCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTAGGTGATAAGGGATCAAGGTACTAAGGACTTGTCTTCTTTATGTTCTCCTCCCTGTTTGGCCAGCTTATTAGAGTTATTTTGCTAATTTTTGGTTGACTTACTTTGAATGAGGTTAAGAAGAAATTTTGGGGTACTGATTGAGTATGAAATACTAACTTTGAACTCTATGGTACGCTACATGCAAGTTTCAAACTTTCCATTCTAATTCGTGGATTATATCTTAGTAGAAGACTTTCTTTTTGTCTTCCATGCCAAAAGGAAAGAAGTGGCTGAGTGAGAGAATGTCAGTGTCTTGTCCTTTCTTCTTGTGTATGTTCATATTTAGAAAGTTTGACAAATTCTATATGCTTTTACTAGAGATTCTTATTCTGGTTTTTTTTATGGGGGAAGGTAATGGTACATTGCTTAAAAAAATATTGCTAGTTGGCATTTTTATGTCATATCTTTGATTCTAAGCTATATTGAGGTGTTGCCTCTATATGACAAATTGATGAATGGGGACCACATCATGTTTTCTGTCCTTAAGAATTAGAAAAACCAAAATTCAACATAAAATAATGATTATTATTATAATTTATGAACCTAACCAATATCAAACATTTACATCATGAAAGGAGGGCTGGTTTCTTCATTATTGGGTAACCAACAAGAATGTGGTCCCAGTTTCAAATGTTTGTGAGTCTGTTTGGAGGTTGTTTTTGGTATTCTTTCATCGTTATCTGAAAACTTGACAAATCCATTCAGTATTGTTGTTATTGATTTTTAGAAAGCCTAGCGGCATAAGCATTTGTATGTAGTTGGAATGATGGAAGTTATGGCGTAATGAGGACATCTAATTTATTATTGAATTATTTTTAATCTTTTCAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCCAACGTAAGGCTACTTTTACATGAACCTATACCTTTCTTTCTAAAGGAAATCTAAATCTAGTTCCACTATGTTCCAGCATGTAAACTTGTAGTAAGCTTGATCGATAAAGTTTTAGGTGTTGTTTAATATGATGAACTAACAAAGTAAATTACAAATTCGGTTGCAGATTAAGTGGATTTGTCCTACTGCTCCTACTCGACCCGTGGCATTATTTGGTGGATTTCCTTGCACTGCTTGTAAGATATTCATCTTGGAATTTCTAATTATCGATCTAGTTGCAAAAAGCCCGGCGTTTCAAATAAGAAAACTCTATTTCTTTTTTTTCTTTTCTTTATTTTAATTGTTAACTGCTATGCTATTATTAAACTTTTATTTTGATAAATATTTTCAAATATGAATGAATTTTTTATAGTTTGTATGCGGGAATCCCTTTATTTGATGTAACTACTTAAGCCGGATGAAAAGAAACTTTCACGTCCGATTTTGAAAGGGGGAGATCCTATAGTATCCTATCCAAATTTTTCTTTTGCTAGGGCCATAGCTAAAAAACCTACTTTCTTATGATTACGGGGTTCATTCGAATATGAAATCCAATCCTGGAAATACAGCATCCCACTTTTTTTTACTACTCAAGGCTTCGATACATTTCGAAATCGAGAAATTTCTACCGGAGCCGGTGCTATACGGCTTTAACAAAACGTAGTTATTAGGGGGTGTTAGGCTCTCAAGATAGGCTTGAAGCCTGACTCAAGGTGAATAAGTGCCTTATGTCTGGTAAGTTGATTTGTTCACTCCAAGCTATGAATTTTTTTAATCTCACAAGGTACACAAGTCAATATTATATAATGGGTTAAAGGGTGTTTGGCCCACAGGTTAGGCTTGTATAACTCAAGGTTAATAAGTCATCCTGTTGGCAAGATGATTTGTTTGTCCCATGCTAGGAATTTTTTAATCTCACAAGGAATTTAAACTCGTATTATATAAACTCAAGTTGATGAAACTCACCTTCCTTCTATGTGCTAGTGTTCTGTGTTTACAAATTAGAGAACTTGTATCAAACAGAAATGATTCAAATCTAGATTAACTTGAAATTATTTTGTGCAATTCAGAAATAAACTACCCAAATCTCGTAGAAAAAATTATTAAATTTGGGAGCCAAATATCTCCTTAGCTTTTAGTGTAAATGAGAAAAATATATTTGTACCTGGAATTAGTTCAACTCAACTTTTTAACATTACGAGGACCTCCCCTGTTTTGTATCAGGGTTTGATGTGGGTGATATTTCTGAAGATTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCACATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATAGTATGTTTCTTCTTGAAGAGCATTTTAACTATCAACTCAACTATTATGTGCTTACCTGTTGAAACTGCTGATACTGAACATCTTCCCCTCATTATATCATCCCTCTCCCCCATCCCTTCCCTTCAGCTGAGCTGCTGTTTGTTACTTAACTTATGCAACTCTTCTAGCATTACTATAATAAGTCGCTATTCGTTTGACCACCCTGCACATTGACCTAATAGTCAATAGGGCAAGCGGACAAAGTAAAGACTTTGGAAGAATGAGTTCAAACCTTACGTGGTTACCTGGTTAGGATGACAGTTTTGTGGTAATCAATATTTGTAGGGTAAATTGGTTGTCCTATGAGATGATTAGTTGAGGCAGGTACTCTAGTCTGAACATCACAAAGATCTAAAATGATAATAATAATTAGAGTCTTTAGTTTTCAACTTTATCATGGCTATAGGGAACTATTATAGTCATTATTTAACTTGATCATCAATATGATACTCCCCTGGTTGGTCAACACATGAGAGAGATGGAATGTCTTGTTGGATTACCCAATATTGTTCTGTCAAGGAGTCAAACCGGCAGTTCTTATATGGTTTAGAAATGAAGCTCAATTATGATTTATGAATTGTAGGTAGATGGGTATAGATATAGATTTCATCTACCGTCTCATATCATTTTTGCAGCTGATACTTGGTGTTTGATGTACAAATTTGTTTTCTTCTCACTCTATCTGATTTAAGATACTTGGAGGACATTCTGTAACCCTCCATTGCCACCCTCACAATCCAAGAATTCCTTTTCCCTCTTGTTAAGATGTAGTTGGGCCATTTTTTCCCCTTTTTCTCCAGTTTTTATTAATTCTACTATGCTTCAACAGTTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCTATTGCAATTTACTCTGCATCATGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCCATTAACCTAAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCTTGTTCAAGGTTTTACTTTTGTTATTTGAACTGTAAAGAGGCAGAAACACACAGTGCAGCCTACATAGGTGGTATTTGTTGTTTAAGTACACTTATTTTTTTTCATCAAATGCTTTTTGTTGTAGATCTTTGAGGAACCAGATCAATGTGTCACATGAAGCTGCAAGGCGGGCTGCGTGTCTACCCATTTTACTCTGTCATGGTTCAGGTACTTGTATTCTTTTTGTATGTTGATAGAAAGATAGTTACCCACAATGGATGCCGACTGAGCACCACATCCCACTTTCTGCTCCATTCATTTTTATATGTTGTAATTTATCACTGATTTATCTGCAATGCTTTTCATCACTGTTCTTGTGAGAAAAATGATTTCTCTCAGTTCCCTTAATCCTACTGACTGCTTATGGACGTGGCAGCAAACCAATTTATTTAGCTTCTCGTCTTTCAGGCTGCACAGTCATTATTCCAAAAAACATTTAGGCTTCTGGATTCTGGTTTTAAGCCTTTAGTATATGCAAGTCACTTAGCCTTAGGAATCTTGAGATTTTCAGTGACTTAACTCAAAAGTGTTTAAGAGAATGATTTTTGAGATTGGAAATGATGGGTGCAAGACTCAATTGTTTTTCTCCATCGACCAAAAGTAACTCTAACCCATCAGATCAACATTACCTGTCTTCCCAAATGCCCATGCTTAATTTTTTGCTTTTATTAAGTACATTCCAATCAAGTTGGAGCTGCTGCTGGATTGAATGGTCCTATATCAAGTGAATTTTGTTCAAGGTACACTTAATTACCATGAGAATCACTTTCATATTACATGCTGTTGGTTACAGGTGATGATGTCGTTGCTTACAAACATGGCGAGAAATCTGCACATACTTTAAGCTCAGCTGGATTTAGAAATCTAACATTTAAAACCTATAATGGGTATGTTAGTATCTTGCTTTCTAGTGCTTCTCGTTTTGTTTGTCTGATAACAAGTAACAAAAAGATGGGATCTTCCCATGCAGGCTGGGACATTACACCATTCCTGAGGAGATGAATGTTGTCTGTAATTGGCTGACAGTAATTTTGGGGCTCGATGGCTTACGGTTATTTGACCACTAA

mRNA sequence

ATGAGCTATAACAGCTCTACTGTGGGCTCTGGTAGTAGAACTGGGAGGATGACCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTAGGTGATAAGGGATCAAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCCAACATTAAGTGGATTTGTCCTACTGCTCCTACTCGACCCGTGGCATTATTTGGTGGATTTCCTTGCACTGCTTGGTTTGATGTGGGTGATATTTCTGAAGATTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCACATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATATTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCTATTGCAATTTACTCTGCATCATGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCCATTAACCTAAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCTTGTTCAAGATCTTTGAGGAACCAGATCAATGTGTCACATGAAGCTGCAAGGCGGGCTGCGTGTCTACCCATTTTACTCTGTCATGGTTCAGGTGATGATGTCGTTGCTTACAAACATGGCGAGAAATCTGCACATACTTTAAGCTCAGCTGGATTTAGAAATCTAACATTTAAAACCTATAATGGGCTGGGACATTACACCATTCCTGAGGAGATGAATGTTGTCTGTAATTGGCTGACAGTAATTTTGGGGCTCGATGGCTTACGGTTATTTGACCACTAA

Coding sequence (CDS)

ATGAGCTATAACAGCTCTACTGTGGGCTCTGGTAGTAGAACTGGGAGGATGACCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTAGGTGATAAGGGATCAAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCCAACATTAAGTGGATTTGTCCTACTGCTCCTACTCGACCCGTGGCATTATTTGGTGGATTTCCTTGCACTGCTTGGTTTGATGTGGGTGATATTTCTGAAGATTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCACATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATATTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCTATTGCAATTTACTCTGCATCATGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCCATTAACCTAAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCTTGTTCAAGATCTTTGAGGAACCAGATCAATGTGTCACATGAAGCTGCAAGGCGGGCTGCGTGTCTACCCATTTTACTCTGTCATGGTTCAGGTGATGATGTCGTTGCTTACAAACATGGCGAGAAATCTGCACATACTTTAAGCTCAGCTGGATTTAGAAATCTAACATTTAAAACCTATAATGGGCTGGGACATTACACCATTCCTGAGGAGATGAATGTTGTCTGTAATTGGCTGACAGTAATTTTGGGGCTCGATGGCTTACGGTTATTTGACCACTAA

Protein sequence

MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADIKLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSHEAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMNVVCNWLTVILGLDGLRLFDH*
Homology
BLAST of Chy3G061220 vs. ExPASy Swiss-Prot
Match: O95372 (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.1e-30
Identity = 82/226 (36.28%), Postives = 121/226 (53.54%), Query Frame = 0

Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVALFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PV L       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEDSPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAAIAIYSA-SCRILGQ 151
           S D+P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A+++Y+A +C     
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----- 141

Query: 152 YGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSHEAARRAACLPILLCHGSGDDVVAYK 211
                  P  L+ +V LS WLP  R+     N S      A  L IL CHG  D +V  +
Sbjct: 142 -------PHPLAGIVALSCWLPLHRAFPQAANGS------AKDLAILQCHGELDPMVPVR 201

Query: 212 HGEKSAHTLSSAGF-RNLTFKTYNGLGHYTIPEEMNVVCNWLTVIL 251
            G  +A  L S      + FKTY G+ H + P+EM  V  +L  +L
Sbjct: 202 FGALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Chy3G061220 vs. ExPASy Swiss-Prot
Match: Q9WTL7 (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.9e-30
Identity = 81/226 (35.84%), Postives = 121/226 (53.54%), Query Frame = 0

Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVALFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PV L       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEDSPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAAIAIYSA-SCRILGQ 151
           S D+P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A+++Y+A +C     
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----- 141

Query: 152 YGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSHEAARRAACLPILLCHGSGDDVVAYK 211
                  P  L+ +V LS WLP  R+     N S      A  L IL CHG  D +V  +
Sbjct: 142 -------PHPLAGIVALSCWLPLHRNFPQAANGS------AKDLAILQCHGELDPMVPVR 201

Query: 212 HGEKSAHTLSSAGF-RNLTFKTYNGLGHYTIPEEMNVVCNWLTVIL 251
            G  +A  L +      + FKTY G+ H + P+EM  V  +L  +L
Sbjct: 202 FGALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Chy3G061220 vs. ExPASy Swiss-Prot
Match: Q9QYL8 (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.9e-30
Identity = 81/226 (35.84%), Postives = 121/226 (53.54%), Query Frame = 0

Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVALFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PV L       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEDSPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAAIAIYSA-SCRILGQ 151
           S D+P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A+++Y+A +C     
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTALTC----- 141

Query: 152 YGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSHEAARRAACLPILLCHGSGDDVVAYK 211
                  P  L+ +V LS WLP  R+     N S      A  L IL CHG  D +V  +
Sbjct: 142 -------PHPLAGIVALSCWLPLHRNFPQAANGS------AKDLAILQCHGELDPMVPVR 201

Query: 212 HGEKSAHTLSSAGF-RNLTFKTYNGLGHYTIPEEMNVVCNWLTVIL 251
            G  +A  L +      + FKTY G+ H + P+EM  V  +L  +L
Sbjct: 202 FGALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

BLAST of Chy3G061220 vs. ExPASy Swiss-Prot
Match: P97823 (Acyl-protein thioesterase 1 OS=Mus musculus OX=10090 GN=Lypla1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.7e-25
Identity = 74/232 (31.90%), Postives = 114/232 (49.14%), Query Frame = 0

Query: 26  VVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVALFGGFPCTAW 85
           VV    K  A +++LHGLGD G  W++    +  P+IK+ICP AP  PV L       +W
Sbjct: 13  VVPAARKATAAVIFLHGLGDTGHGWAEAFAGIKSPHIKYICPHAPVMPVTLNMNMAMPSW 72

Query: 86  FDVGDISEDSPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAAIAIYSASC 145
           FD+  +S DS +D  G+  AA  V  L+  E     P++ ++ +GGFS G A+++Y+A  
Sbjct: 73  FDIVGLSPDSQEDESGIKQAAETVKALIDQEVKNGIPSN-RIILGGFSQGGALSLYTALT 132

Query: 146 RILGQYGNGNLYPINLSAVVGLSGWLPCSRSL-RNQINVSHEAARRAACLPILLCHGSGD 205
                          L+ V  LS WLP   S  +  IN ++        + +L CHG  D
Sbjct: 133 T-----------QQKLAGVTALSCWLPLRASFSQGPINSANRD------ISVLQCHGDCD 192

Query: 206 DVVAYKHGEKSAHTLSS-AGFRNLTFKTYNGLGHYTIPEEMNVVCNWLTVIL 251
            +V    G  +   L +     N+TFK Y G+ H +  +EM  V +++  +L
Sbjct: 193 PLVPLMFGSLTVERLKALINPANVTFKIYEGMMHSSCQQEMMDVKHFIDKLL 226

BLAST of Chy3G061220 vs. ExPASy Swiss-Prot
Match: O77821 (Acyl-protein thioesterase 1 OS=Oryctolagus cuniculus OX=9986 GN=LYPLA1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.7e-25
Identity = 74/232 (31.90%), Postives = 114/232 (49.14%), Query Frame = 0

Query: 26  VVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVALFGGFPCTAW 85
           VV    K  A +++LHGLGD G  W++    +  P+IK+ICP AP  PV L       +W
Sbjct: 13  VVPAARKATAAVIFLHGLGDTGHGWAEAFAGIKSPHIKYICPHAPVMPVTLNMNMAMPSW 72

Query: 86  FDVGDISEDSPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAAIAIYSASC 145
           FD+  +S DS +D  G+  AA  V  L+  E     P++ ++ +GGFS G A+++Y+A  
Sbjct: 73  FDIVGLSPDSQEDESGIKQAAETVKALIDQEVKNGIPSN-RIILGGFSQGGALSLYTALT 132

Query: 146 RILGQYGNGNLYPINLSAVVGLSGWLPCSRSL-RNQINVSHEAARRAACLPILLCHGSGD 205
                          L+ V  LS WLP   S  +  IN ++        + +L CHG  D
Sbjct: 133 T-----------QQKLAGVTALSCWLPLRASFSQGPINSANRD------ISVLQCHGDCD 192

Query: 206 DVVAYKHGEKSAHTLSS-AGFRNLTFKTYNGLGHYTIPEEMNVVCNWLTVIL 251
            +V    G  +   L +     N+TFK Y G+ H +  +EM  V +++  +L
Sbjct: 193 PLVPLMFGSLTVERLKALINPANVTFKIYEGMMHSSCQQEMMDVKHFIDKLL 226

BLAST of Chy3G061220 vs. ExPASy TrEMBL
Match: A0A0A0LM90 (Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G380030 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 5.3e-150
Identity = 257/260 (98.85%), Postives = 258/260 (99.23%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQ+LETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQILETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN
Sbjct: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240

Query: 241 VVCNWLTVILGLDGLRLFDH 261
           VVCNWLTVILGLDGLRL DH
Sbjct: 241 VVCNWLTVILGLDGLRLVDH 260

BLAST of Chy3G061220 vs. ExPASy TrEMBL
Match: A0A1S4DVB8 (acyl-protein thioesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103488285 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.3e-145
Identity = 251/260 (96.54%), Postives = 252/260 (96.92%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEP DI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPGDI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQI VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQIGVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAA RAA LPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFK YNGLGHYTIPEEM+
Sbjct: 181 EAAMRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKAYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 261
           VVC WLTVILGLDGLRL DH
Sbjct: 241 VVCQWLTVILGLDGLRLVDH 260

BLAST of Chy3G061220 vs. ExPASy TrEMBL
Match: A0A6J1HB89 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111462433 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 7.4e-144
Identity = 245/260 (94.23%), Postives = 254/260 (97.69%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 261
           VVCNWLTV+LGLDGLRL DH
Sbjct: 241 VVCNWLTVVLGLDGLRLVDH 260

BLAST of Chy3G061220 vs. ExPASy TrEMBL
Match: A0A6J1K2A0 (acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111491767 PE=4 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 1.3e-143
Identity = 245/260 (94.23%), Postives = 254/260 (97.69%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSST+GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 261
           VVCNWLTVIL LDG+RL DH
Sbjct: 241 VVCNWLTVILALDGIRLVDH 260

BLAST of Chy3G061220 vs. ExPASy TrEMBL
Match: A0A6J1BUH3 (acyl-protein thioesterase 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004864 PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 7.0e-142
Identity = 244/260 (93.85%), Postives = 253/260 (97.31%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSTVGSGSRTGRMT EFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTLEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSA+CRILGQYGNGNLYPI+LSAVVGLSGWLPCSRSLRNQI+VSH
Sbjct: 121 KLGIGGFSMGAATAIYSATCRILGQYGNGNLYPIHLSAVVGLSGWLPCSRSLRNQISVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAAR AA LPILLCHGSGDDVVA+KHGE+SA TLSSAGFRNLTF+TY+GLGHYTIPEEM+
Sbjct: 181 EAARHAAALPILLCHGSGDDVVAFKHGERSAQTLSSAGFRNLTFRTYHGLGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 261
           VVCNWLT ILGLDGLRL D+
Sbjct: 241 VVCNWLTAILGLDGLRLVDN 260

BLAST of Chy3G061220 vs. NCBI nr
Match: XP_004138733.1 (acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649812.1 acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649813.1 acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649814.1 acyl-protein thioesterase 2 [Cucumis sativus] >KGN62923.1 hypothetical protein Csa_021920 [Cucumis sativus])

HSP 1 Score: 540 bits (1390), Expect = 8.71e-194
Identity = 257/260 (98.85%), Postives = 258/260 (99.23%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQ+LETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQILETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN
Sbjct: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240

Query: 241 VVCNWLTVILGLDGLRLFDH 260
           VVCNWLTVILGLDGLRL DH
Sbjct: 241 VVCNWLTVILGLDGLRLVDH 260

BLAST of Chy3G061220 vs. NCBI nr
Match: XP_038886103.1 (acyl-protein thioesterase 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 528 bits (1361), Expect = 2.30e-189
Identity = 253/260 (97.31%), Postives = 256/260 (98.46%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQI++SH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQISMSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYN LGHYTIPEEM+
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNELGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 260
           VVCNWLTVILGLDGLRL DH
Sbjct: 241 VVCNWLTVILGLDGLRLVDH 260

BLAST of Chy3G061220 vs. NCBI nr
Match: XP_008445177.1 (PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445178.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445179.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445180.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_016899931.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo])

HSP 1 Score: 524 bits (1350), Expect = 1.09e-187
Identity = 251/260 (96.54%), Postives = 252/260 (96.92%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEP DI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPGDI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQI VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQIGVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAA RAA LPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFK YNGLGHYTIPEEM+
Sbjct: 181 EAAMRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKAYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 260
           VVC WLTVILGLDGLRL DH
Sbjct: 241 VVCQWLTVILGLDGLRLVDH 260

BLAST of Chy3G061220 vs. NCBI nr
Match: KAG6598584.1 (Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 521 bits (1343), Expect = 1.28e-186
Identity = 246/260 (94.62%), Postives = 255/260 (98.08%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GFRNLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFRNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 260
           VVCNWLTV+LGLDGLRL DH
Sbjct: 241 VVCNWLTVVLGLDGLRLVDH 260

BLAST of Chy3G061220 vs. NCBI nr
Match: XP_022961761.1 (acyl-protein thioesterase 2-like [Cucurbita moschata])

HSP 1 Score: 519 bits (1337), Expect = 1.05e-185
Identity = 245/260 (94.23%), Postives = 254/260 (97.69%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLGIGGFSMGAA AIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVILGLDGLRLFDH 260
           VVCNWLTV+LGLDGLRL DH
Sbjct: 241 VVCNWLTVVLGLDGLRLVDH 260

BLAST of Chy3G061220 vs. TAIR 10
Match: AT3G15650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 3.6e-106
Identity = 181/256 (70.70%), Postives = 214/256 (83.59%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSY+  ++GSGSR+ R  +EFGRT+VVRPKGKHQATIVWLHGLGD GSS SQLLE+LPLP
Sbjct: 1   MSYSRQSMGSGSRSTR-GYEFGRTYVVRPKGKHQATIVWLHGLGDNGSSSSQLLESLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP+RPV+L GGFPCTAWFDVG+ISED  DD+EGLDA+A+H+ANLLS EP D+
Sbjct: 61  NIKWICPTAPSRPVSLLGGFPCTAWFDVGEISEDLHDDIEGLDASAAHIANLLSAEPTDV 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           K+GIGGFSMGAAIA+YS +C  LG+YG G+ Y INL A VGLSGWLP  RSLR++I  S+
Sbjct: 121 KVGIGGFSMGAAIALYSTTCYALGRYGTGHAYTINLRATVGLSGWLPGWRSLRSKIESSN 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           E ARRAA +PILL HG+ DDVV Y+ GEKSAH+L+ AGFR   FK Y GLGHYT+P+EM+
Sbjct: 181 EVARRAASIPILLAHGTSDDVVPYRFGEKSAHSLAMAGFRQTMFKPYEGLGHYTVPKEMD 240

Query: 241 VVCNWLTVILGLDGLR 257
            V +WL   LGL+G R
Sbjct: 241 EVVHWLVSRLGLEGSR 255

BLAST of Chy3G061220 vs. TAIR 10
Match: AT1G52700.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 2.3e-105
Identity = 180/256 (70.31%), Postives = 213/256 (83.20%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSY+  ++GSGSR  R  +EFGRT+VVRPKGKHQAT+VWLHGLGD GSS SQL+++L LP
Sbjct: 1   MSYSHQSMGSGSRNAR-GYEFGRTYVVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP+RPV   GGF CTAWFDVG+ISED  DDLEGLDA+ASH+ANLLS+EPAD+
Sbjct: 61  NIKWICPTAPSRPVTSLGGFTCTAWFDVGEISEDGHDDLEGLDASASHIANLLSSEPADV 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           K+GIGGFSMGAAI++YSA+C  LG+YG G+ YPINL AVVGLSGWLP  +SLR++I  S 
Sbjct: 121 KVGIGGFSMGAAISLYSATCYALGRYGTGHAYPINLQAVVGLSGWLPGWKSLRSKIECSF 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           EAARRAA LPI+L HG+ DDVV Y+ GEKSA +L  AGFR   FK Y GLGHYT+P EM+
Sbjct: 181 EAARRAASLPIILTHGTSDDVVPYRFGEKSAQSLGMAGFRLAMFKPYEGLGHYTVPREMD 240

Query: 241 VVCNWLTVILGLDGLR 257
            V +WLT +LGL+G R
Sbjct: 241 EVVHWLTTMLGLEGSR 255

BLAST of Chy3G061220 vs. TAIR 10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 4.4e-104
Identity = 173/254 (68.11%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MS + + VGSG R  R   EFG+THVVRPKGKHQATIVWLHGLGD GSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGSG-RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP++P++LFGGFP TAWFDV DI+ED PDD+EGLD AA+HVANLLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLG+GGFSMGAA ++YSA+C  LG+YGNGN YPINLSA++GLSGWLPC+++L  ++    
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLE-EE 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           +   RAA LPI++CHG  DDVV +K GEKS+  L S GF+ +TFK Y+ LGH+TIP+E++
Sbjct: 181 QIKNRAASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 VVCNWLTVILGLDG 255
            +C WLT  L L+G
Sbjct: 241 ELCAWLTSTLSLEG 252

BLAST of Chy3G061220 vs. TAIR 10
Match: AT5G20060.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 4.4e-104
Identity = 173/254 (68.11%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MS + + VGSG R  R   EFG+THVVRPKGKHQATIVWLHGLGD GSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGSG-RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP++P++LFGGFP TAWFDV DI+ED PDD+EGLD AA+HVANLLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLG+GGFSMGAA ++YSA+C  LG+YGNGN YPINLSA++GLSGWLPC+++L  ++    
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLE-EE 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           +   RAA LPI++CHG  DDVV +K GEKS+  L S GF+ +TFK Y+ LGH+TIP+E++
Sbjct: 181 QIKNRAASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 VVCNWLTVILGLDG 255
            +C WLT  L L+G
Sbjct: 241 ELCAWLTSTLSLEG 252

BLAST of Chy3G061220 vs. TAIR 10
Match: AT5G20060.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 375.2 bits (962), Expect = 4.4e-104
Identity = 173/254 (68.11%), Postives = 209/254 (82.28%), Query Frame = 0

Query: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MS + + VGSG R  R   EFG+THVVRPKGKHQATIVWLHGLGD GSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGSG-RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP++P++LFGGFP TAWFDV DI+ED PDD+EGLD AA+HVANLLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGIGGFSMGAAIAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180
           KLG+GGFSMGAA ++YSA+C  LG+YGNGN YPINLSA++GLSGWLPC+++L  ++    
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLE-EE 180

Query: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240
           +   RAA LPI++CHG  DDVV +K GEKS+  L S GF+ +TFK Y+ LGH+TIP+E++
Sbjct: 181 QIKNRAASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 VVCNWLTVILGLDG 255
            +C WLT  L L+G
Sbjct: 241 ELCAWLTSTLSLEG 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O953723.1e-3036.28Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1[more]
Q9WTL76.9e-3035.84Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1[more]
Q9QYL86.9e-3035.84Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1[more]
P978236.7e-2531.90Acyl-protein thioesterase 1 OS=Mus musculus OX=10090 GN=Lypla1 PE=1 SV=1[more]
O778216.7e-2531.90Acyl-protein thioesterase 1 OS=Oryctolagus cuniculus OX=9986 GN=LYPLA1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LM905.3e-15098.85Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G3800... [more]
A0A1S4DVB82.3e-14596.54acyl-protein thioesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103488285 PE=4 SV... [more]
A0A6J1HB897.4e-14494.23acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111462433 P... [more]
A0A6J1K2A01.3e-14394.23acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111491767 PE=... [more]
A0A6J1BUH37.0e-14293.85acyl-protein thioesterase 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_004138733.18.71e-19498.85acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649812.1 acyl-protein thioe... [more]
XP_038886103.12.30e-18997.31acyl-protein thioesterase 2-like isoform X2 [Benincasa hispida][more]
XP_008445177.11.09e-18796.54PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445178.1 PREDI... [more]
KAG6598584.11.28e-18694.62Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961761.11.05e-18594.23acyl-protein thioesterase 2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G15650.13.6e-10670.70alpha/beta-Hydrolases superfamily protein [more]
AT1G52700.12.3e-10570.31alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.24.4e-10468.11alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.14.4e-10468.11alpha/beta-Hydrolases superfamily protein [more]
AT5G20060.34.4e-10468.11alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 17..251
e-value: 4.5E-61
score: 208.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 25..247
IPR003140Phospholipase/carboxylesterase/thioesterasePFAMPF02230Abhydrolase_2coord: 25..247
e-value: 8.1E-40
score: 136.9
NoneNo IPR availablePANTHERPTHR46234:SF3ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..254
NoneNo IPR availablePANTHERPTHR46234ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G061220.1Chy3G061220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity