Chy3G059920 (gene) Cucumber (hystrix) v1

Overview
NameChy3G059920
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioneukaryotic translation initiation factor 5-like
LocationchrH03: 13669527 .. 13670861 (-)
RNA-Seq ExpressionChy3G059920
SyntenyChy3G059920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTGCAGAATATTGGTGCTTCAAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAGGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTCGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACACGAGACTGCAAAGCTGGCTGGTCTTCTTGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTCGACATGAGAGATAAACTCACAACATTTATTCTCAAGAACCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATGTTCTAAAAAAGAAAAGCACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGACGAAGATGATGATGACGTGCAGTGGCAAACGGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCAGATATGGTCATGCTCTCAACTACTGAAGAGAACAAGTCGTCAAAGAAGTCATCTGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGAGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTGCCTCCACAACTGATCTCAAGTCATTCTTGGAATCACTCACTGGAACTCGCCAAGAGATTGTGAACGCCTTAGTCGAAGCTCTATTTGATGGTGTTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAAAAGAAGAAGTTCCTGGCTGTAGTTGCTGCTGTGGAAGGATCACAGATAATTCTTTTACGTGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCACTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCGTTCATCGAATGGCTCCAAAATGCCGAGTCGGAGACGGAAGAAGAGTAA

mRNA sequence

ATGGCGTTGCAGAATATTGGTGCTTCAAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAGGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTCGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACACGAGACTGCAAAGCTGGCTGGTCTTCTTGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTCGACATGAGAGATAAACTCACAACATTTATTCTCAAGAACCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATGTTCTAAAAAAGAAAAGCACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGACGAAGATGATGATGACGTGCAGTGGCAAACGGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCAGATATGGTCATGCTCTCAACTACTGAAGAGAACAAGTCGTCAAAGAAGTCATCTGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGAGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTGCCTCCACAACTGATCTCAAGTCATTCTTGGAATCACTCACTGGAACTCGCCAAGAGATTGTGAACGCCTTAGTCGAAGCTCTATTTGATGGTGTTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAAAAGAAGAAGTTCCTGGCTGTAGTTGCTGCTGTGGAAGGATCACAGATAATTCTTTTACGTGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCACTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCGTTCATCGAATGGCTCCAAAATGCCGAGTCGGAGACGGAAGAAGAGTAA

Coding sequence (CDS)

ATGGCGTTGCAGAATATTGGTGCTTCAAACCGCGATGATGCCTTCTATAGGTATAAAATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAGGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTTGGATGTGAACTTGGTGCACAATCCAAGTTCGATGAGAAAACTGGAACTTCTCTTGTAAATGGTGCACACGAGACTGCAAAGCTGGCTGGTCTTCTTGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGTAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTCGACATGAGAGATAAACTCACAACATTTATTCTCAAGAACCCACCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGGCTTAAGGAGGGAGAGGCTGCCGATGAGGAGCAGAAGAAAATAAAAAAGGATGTTCTAAAAAAGAAAAGCACTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAAGATCATTCTCCAACTCGTAGCCATGCTGATGATAATGACCGAGCAGCTGATGACGAAGATGATGATGACGTGCAGTGGCAAACGGATACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGTGCTGTCACTGCAGATATGGTCATGCTCTCAACTACTGAAGAGAACAAGTCGTCAAAGAAGTCATCTGAGCATGAGAAGTCTGCAGCCAATGGTAACAAGAATGGAAATGGAGTTTCTACACATGGTGCCCTCATTCAAGAAATCAAAGATTATTTGAAGAAAGGTGCCTCCACAACTGATCTCAAGTCATTCTTGGAATCACTCACTGGAACTCGCCAAGAGATTGTGAACGCCTTAGTCGAAGCTCTATTTGATGGTGTTGGCAAAGGCTTCTCTAAGGAAGTAGTGAAAAAGAAGAAGTTCCTGGCTGTAGTTGCTGCTGTGGAAGGATCACAGATAATTCTTTTACGTGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCGGGTAAGGAGGTTGCCCTTGTCCTGAAATCACTATACGATGGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAGCAGGGTGTGGCTGGTGCAAACAAAAGCTCTCAAATCTGGAAATACGTAAAGCCGTTCATCGAATGGCTCCAAAATGCCGAGTCGGAGACGGAAGAAGAGTAA

Protein sequence

MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEEE*
Homology
BLAST of Chy3G059920 vs. ExPASy Swiss-Prot
Match: P48724 (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 1.6e-159
Identity = 306/448 (68.30%), Postives = 356/448 (79.46%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGA N DDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAK L RPASYTTKYFG
Sbjct: 1   MALQNIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTS VNGAHETAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI 
Sbjct: 61  CELGAQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKV 180

Query: 181 KKDVLKKKSTTTKDAVTK-VPSKKKNNGSDED-HSPTRSHADDNDRAADDEDDDD--VQW 240
           KK+V KK S++ KD   K   SKKK +GSDED  SPT    ++ + A D++DDDD  VQW
Sbjct: 181 KKEVKKKGSSSAKDGTAKSTISKKKGSGSDEDRRSPTHKQIEEKEEAKDEDDDDDDGVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG 300
            TDTSL+A++QRI+EQLSAVTADMVML+T E  K  K +S     + NGN        +G
Sbjct: 241 LTDTSLDASRQRIKEQLSAVTADMVMLTTDEPEKKKKAASNQNGGSQNGNS-----KNYG 300

Query: 301 ALIQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360
            ++ E+K  LKKG   ++L S L +L    QE ++ALVEALF+G  KGF +E +KKK + 
Sbjct: 301 TVVAEVKANLKKGFGASELLSHLAALPVPAQEKMSALVEALFEGTEKGFGRETLKKKNYF 360

Query: 361 AVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
           A   A EGSQI+LL AIE FC   +  A KEVALVLK+LYD DV+EEE ++ WYQ+G+ G
Sbjct: 361 AAAVAEEGSQILLLHAIEEFCCKPNSNALKEVALVLKTLYDADVLEEEAIVLWYQKGLKG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 445
            NK+S+IWK  +PFI+WLQNAESE++EE
Sbjct: 421 DNKNSKIWKNAQPFIDWLQNAESESDEE 443

BLAST of Chy3G059920 vs. ExPASy Swiss-Prot
Match: P55876 (Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.4e-155
Identity = 310/454 (68.28%), Postives = 359/454 (79.07%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMV+IAKAL RPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG SLVNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI+
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMIS 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKKG KDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKN--NGSDEDH--SPTRSHADDNDRAADDEDDDDVQW 240
           KKD  KK S   KD+  K   KK     GSDEDH  SPTRSH  D D+AA D+DDDDVQW
Sbjct: 181 KKDAKKKGS---KDSTAKGLKKKATTATGSDEDHSSSPTRSH--DGDKAAADDDDDDVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNG------- 300
           QTDTS+EAAKQR+QEQLSA TA+MVMLS TEE +   K   H+  + NG+          
Sbjct: 241 QTDTSIEAAKQRMQEQLSAATAEMVMLS-TEETEKKMKQPTHKDGSTNGSAKEIPNDKPA 300

Query: 301 -NGVSTHGALIQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKE 360
               S +  LI +IK  L    + + LK+ L S T   Q+++NA +EALF GVGKGF+KE
Sbjct: 301 VTKPSPYEELIGDIKASLGSAPTPSQLKAVLASSTLPPQDVMNAPLEALFGGVGKGFTKE 360

Query: 361 VVKKKKFLAVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLE 420
           VVK KK+LAV    EG+Q +L++AIE+F    +PEA KEV +VLK+LYDGD+++EE +++
Sbjct: 361 VVKNKKYLAVAVPDEGAQTLLVQAIEAFGGKCNPEALKEVPVVLKALYDGDILDEETIVD 420

Query: 421 WYQQGVAGANKSSQIWKYVKPFIEWLQNAESETE 443
           WY   VA A K SQ+ K  KPF+EWLQ+AES+ E
Sbjct: 421 WYNDAVA-AGKDSQVVKNAKPFVEWLQSAESDEE 447

BLAST of Chy3G059920 vs. ExPASy Swiss-Prot
Match: Q9C8F1 (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX=3702 GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.8e-155
Identity = 303/453 (66.89%), Postives = 361/453 (79.69%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDVL-KKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KK+   KKK+ TT  +  KV  KK       DHSP RS +D+ND+A  +EDDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNG---VSTHG 300
           TS EAA++R++EQLSAVTA+MVMLST EE    K  +E +K+    ++NGN     + H 
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEE---KKPVAEVKKAPEQVHENGNSKIPENAHE 300

Query: 301 ALIQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360
            L+ EIK+ L  G+S T LK+ L S +   QE ++AL  ALF G GKGF+KEV+KKKK+L
Sbjct: 301 KLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKKKYL 360

Query: 361 AVVAAVE-----GSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQ 420
             +  ++      +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ ++EWY 
Sbjct: 361 LALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVEWYN 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEEE 445
           +GV    KSS + K V PFIEWLQNAESE+EEE
Sbjct: 421 KGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

BLAST of Chy3G059920 vs. ExPASy Swiss-Prot
Match: Q9S825 (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX=3702 GN=At1g77840 PE=1 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 9.3e-152
Identity = 304/449 (67.71%), Postives = 358/449 (79.73%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDD-VQWQTD 240
           K    KK  +  KD+ T      KN+ SDED SP   H ++     +DEDDDD V+WQTD
Sbjct: 181 K--AKKKALSNGKDSKT-----SKNHSSDEDISP--KHDENALEVDEDEDDDDGVEWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTE-ENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300
           TS EAA++R+ EQLSA TA+MVMLS  E E K + KS    KS  N  K  N       L
Sbjct: 241 TSREAAEKRMMEQLSAKTAEMVMLSAMEVEEKKAPKS----KSNGNVVKTENPPPQEKNL 300

Query: 301 IQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360
           +Q++K+YLKKG+  + LKSF+ SL+   Q+I++AL  ALFDGVGKGF+KEV KKK +LA 
Sbjct: 301 VQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLAA 360

Query: 361 VAAV--EGSQIILLRAIESFC-LNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVA 420
            A +  +GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL+WY++G+ 
Sbjct: 361 AATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGLT 420

Query: 421 GANKSSQIWKYVKPFIEWLQNAESETEEE 445
           GA+KSS +WK VKPF+EWLQ+AESE+EEE
Sbjct: 421 GADKSSPVWKNVKPFVEWLQSAESESEEE 436

BLAST of Chy3G059920 vs. ExPASy Swiss-Prot
Match: P59325 (Eukaryotic translation initiation factor 5 OS=Mus musculus OX=10090 GN=Eif5 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.3e-46
Identity = 149/450 (33.11%), Postives = 218/450 (48.44%), Query Frame = 0

Query: 13  DAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEK 72
           D FYRYKMP+++ K+EG+GNGIKT +VNMV++AKAL RP +Y TKYFGCELGAQ++FD K
Sbjct: 11  DQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK 70

Query: 73  TGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMITMKCAACGFISD 132
               +VNG+HE  KL  +L+ FIKK+V C  C NPET++ +  K Q I   C ACG+   
Sbjct: 71  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGM 130

Query: 133 VDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDVLKKKSTT 192
           +D   KL TFILKNPPE       KK                 E++KK +K         
Sbjct: 131 LDTHHKLCTFILKNPPENSDIGTGKK-----------------EKEKKNRK--------- 190

Query: 193 TKDAVTKVPSKKKNNGS---DEDHSPTRSHADDNDRAADDEDDDDVQWQTDTSLEAAKQR 252
                     K K NGS    E   P   +      A ++E+DDD  W  DT+ EA ++R
Sbjct: 191 ---------GKDKENGSVSTSETPPPPPPNEISPPHAVEEEEDDD--WGEDTTEEAQRRR 250

Query: 253 IQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQEIKDYLKK 312
           + E              ++  K    S + E++                 +  + D++KK
Sbjct: 251 MDE-------------ISDHAKGLTLSDDLERTVEE-------------RVNILFDFVKK 310

Query: 313 GASTTDLKSFLESLTGTRQEI-VNA-----LVEALFDGVGKGFSKEVVKK--KKFLAVVA 372
                 + S  + +    + + V A     L E LFD       +E +KK  + FL    
Sbjct: 311 KKEEGIIDSSDKEIVAEAERLDVKAMGPLVLTEVLFDEK----IREQIKKYRRHFLRFCH 370

Query: 373 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQG---VAGA 432
             + +Q  LL  +E        +   ++  +LK +YD D++EEE ++ W ++        
Sbjct: 371 NNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSK 393

Query: 433 NKSSQIWKYVKPFIEWLQNAESET---EEE 445
             + +I    +PFI+WL+ AE E+   EEE
Sbjct: 431 ELAKEIRVKAEPFIKWLKEAEEESSGGEEE 393

BLAST of Chy3G059920 vs. ExPASy TrEMBL
Match: A0A0A0LMB4 (W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.5e-240
Identity = 443/444 (99.77%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKDYLKKGASTTDLKSFLESL GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS
Sbjct: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 445
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Chy3G059920 vs. ExPASy TrEMBL
Match: A0A1S3BCI3 (eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC103488416 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.5e-234
Identity = 432/444 (97.30%), Postives = 440/444 (99.10%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHA DND+AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTH +LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           A EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 AEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 445
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Chy3G059920 vs. ExPASy TrEMBL
Match: A0A5A7VCS9 (Eukaryotic translation initiation factor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001250 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 5.9e-234
Identity = 431/444 (97.07%), Postives = 439/444 (98.87%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSP RSHA DND AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDLAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG+LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           + EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 SEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 445
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Chy3G059920 vs. ExPASy TrEMBL
Match: A0A6J1BQ30 (eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 GN=LOC111004721 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.4e-219
Identity = 409/448 (91.29%), Postives = 426/448 (95.09%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDVLKKKST--TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAA-DDEDDDDVQWQ 240
           KKD +KKKST   TKDAVTKVPSKKKNNGSDEDHSPTRS A DND AA DDED+DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS+E+EKS  NGNKNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LIQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA 360
           L+ EIKDYLKKG+S  +LKSFLESL+GTRQE+VNALVE LFDGVGKGFSKEV+KKK FLA
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFLA 360

Query: 361 VVAAVEG-SQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
             AA EG SQIILLRAIES CLNT+PEAGKEVALVLKSLYDGDV+EEEFVLEWYQQG+AG
Sbjct: 361 AAAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 445
            NKSSQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ENKSSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Chy3G059920 vs. ExPASy TrEMBL
Match: A0A6J1HCN3 (probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3662 GN=LOC111462865 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.3e-216
Identity = 401/448 (89.51%), Postives = 419/448 (93.53%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG +LVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMI 
Sbjct: 61  CELGAQSKFDEKTGIALVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQT 240
           KKD LKKKST+   K+AVTKVPSKKK+NGSDEDHSPTRSHA +ND+AADD+DDDDVQWQT
Sbjct: 181 KKDSLKKKSTSGNAKEAVTKVPSKKKSNGSDEDHSPTRSHAVENDQAADDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEE KSSKKS EHEKS  NG+KNGNGVSTHGAL
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEETKSSKKSPEHEKSVVNGSKNGNGVSTHGAL 300

Query: 301 IQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360
           + EIKDYLKK  S  DLKSFLESLTGT QE  NALVEALF+GVGKGFSKEVVKKKKFL  
Sbjct: 301 VHEIKDYLKKSPSAADLKSFLESLTGTHQETANALVEALFEGVGKGFSKEVVKKKKFLVA 360

Query: 361 VAAV--EGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
           VAA   EGSQI+LLR+IESFCLNT PE  KE+ALVLKSLYDGDVIEEEFVLEWYQQG+AG
Sbjct: 361 VAAAQEEGSQIVLLRSIESFCLNTCPEVAKEIALVLKSLYDGDVIEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 445
           ANK SQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ANKGSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Chy3G059920 vs. NCBI nr
Match: XP_004150695.1 (eukaryotic translation initiation factor 5 [Cucumis sativus] >XP_011649412.1 eukaryotic translation initiation factor 5 [Cucumis sativus] >KAE8652938.1 hypothetical protein Csa_017777 [Cucumis sativus])

HSP 1 Score: 837 bits (2162), Expect = 3.08e-305
Identity = 443/444 (99.77%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKDYLKKGASTTDLKSFLESL GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS
Sbjct: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Chy3G059920 vs. NCBI nr
Match: XP_008445359.1 (PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo] >XP_008445360.1 PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo])

HSP 1 Score: 818 bits (2112), Expect = 1.28e-297
Identity = 432/444 (97.30%), Postives = 440/444 (99.10%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHA DND+AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTH +LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHVSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           A EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 AEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Chy3G059920 vs. NCBI nr
Match: KAA0064817.1 (eukaryotic translation initiation factor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 817 bits (2110), Expect = 2.59e-297
Identity = 431/444 (97.07%), Postives = 439/444 (98.87%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTDT 240
           KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSP RSHA DND AADDEDDDDVQWQTDT
Sbjct: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDLAADDEDDDDVQWQTDT 240

Query: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALIQ 300
           SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHG+LIQ
Sbjct: 241 SLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGSLIQ 300

Query: 301 EIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360
           EIKD+LKKG+STTDLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA
Sbjct: 301 EIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVVA 360

Query: 361 AVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANKS 420
           + EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYDGDVIEEEFVLEWY+QGVAGANKS
Sbjct: 361 SEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAGANKS 420

Query: 421 SQIWKYVKPFIEWLQNAESETEEE 444
           SQIWKYVKPFIEWLQNAESETEEE
Sbjct: 421 SQIWKYVKPFIEWLQNAESETEEE 444

BLAST of Chy3G059920 vs. NCBI nr
Match: XP_038884689.1 (eukaryotic translation initiation factor 5-like [Benincasa hispida])

HSP 1 Score: 798 bits (2062), Expect = 5.74e-290
Identity = 424/445 (95.28%), Postives = 431/445 (96.85%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKA+RRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKALRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDVLKKKST-TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KKD LKKKST TTKDAVTKVPSKKKNNGSDEDHSP RSHA DND+AADD+DDDDVQWQTD
Sbjct: 181 KKDSLKKKSTGTTKDAVTKVPSKKKNNGSDEDHSPARSHAGDNDQAADDDDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGALI 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENK SKKS EHEKSA NGNKNGNGVSTHGALI
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKLSKKSPEHEKSAVNGNKNGNGVSTHGALI 300

Query: 301 QEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAVV 360
           QEIKDYLKKG S  DLKSFLESL+GTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA  
Sbjct: 301 QEIKDYLKKGPSAADLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLASA 360

Query: 361 AAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAGANK 420
           AA EGSQIILLRAIESFC+NTSPEAGKEVALVLKSLYDGDVI+EEFVLEWYQQGVAGANK
Sbjct: 361 AAEEGSQIILLRAIESFCINTSPEAGKEVALVLKSLYDGDVIDEEFVLEWYQQGVAGANK 420

Query: 421 SSQIWKYVKPFIEWLQNAESETEEE 444
           SS IWK+VKPFIEWLQNAESETEEE
Sbjct: 421 SSHIWKHVKPFIEWLQNAESETEEE 445

BLAST of Chy3G059920 vs. NCBI nr
Match: XP_022131574.1 (eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131575.1 eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131576.1 eukaryotic translation initiation factor 5-like [Momordica charantia])

HSP 1 Score: 768 bits (1984), Expect = 4.90e-278
Identity = 409/448 (91.29%), Postives = 426/448 (95.09%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDVLKKKSTT--TKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADD-EDDDDVQWQ 240
           KKD +KKKST   TKDAVTKVPSKKKNNGSDEDHSPTRS A DND AADD ED+DDVQWQ
Sbjct: 181 KKDSVKKKSTAGNTKDAVTKVPSKKKNNGSDEDHSPTRSQAGDNDHAADDDEDEDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNGVSTHGA 300
           TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS+E+EKS  NGNKNGNG+S HGA
Sbjct: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSTENEKSQVNGNKNGNGISAHGA 300

Query: 301 LIQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLA 360
           L+ EIKDYLKKG+S  +LKSFLESL+GTRQE+VNALVE LFDGVGKGFSKEV+KKK FLA
Sbjct: 301 LVHEIKDYLKKGSSAAELKSFLESLSGTRQEVVNALVECLFDGVGKGFSKEVLKKKSFLA 360

Query: 361 VVAAVEGS-QIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420
             AA EGS QIILLRAIES CLNT+PEAGKEVALVLKSLYDGDV+EEEFVLEWYQQG+AG
Sbjct: 361 AAAAQEGSSQIILLRAIESLCLNTNPEAGKEVALVLKSLYDGDVLEEEFVLEWYQQGLAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444
            NKSSQIWK+VKPFIEWLQNAESETEEE
Sbjct: 421 ENKSSQIWKHVKPFIEWLQNAESETEEE 448

BLAST of Chy3G059920 vs. TAIR 10
Match: AT1G36730.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 550.4 bits (1417), Expect = 1.3e-156
Identity = 303/453 (66.89%), Postives = 361/453 (79.69%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDVL-KKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240
           KK+   KKK+ TT  +  KV  KK       DHSP RS +D+ND+A  +EDDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKSSEHEKSAANGNKNGNG---VSTHG 300
           TS EAA++R++EQLSAVTA+MVMLST EE    K  +E +K+    ++NGN     + H 
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEE---KKPVAEVKKAPEQVHENGNSKIPENAHE 300

Query: 301 ALIQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360
            L+ EIK+ L  G+S T LK+ L S +   QE ++AL  ALF G GKGF+KEV+KKKK+L
Sbjct: 301 KLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKKKYL 360

Query: 361 AVVAAVE-----GSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQ 420
             +  ++      +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ ++EWY 
Sbjct: 361 LALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVEWYN 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEEE 445
           +GV    KSS + K V PFIEWLQNAESE+EEE
Sbjct: 421 KGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

BLAST of Chy3G059920 vs. TAIR 10
Match: AT1G77840.1 (Translation initiation factor IF2/IF5 )

HSP 1 Score: 538.1 bits (1385), Expect = 6.6e-153
Identity = 304/449 (67.71%), Postives = 358/449 (79.73%), Query Frame = 0

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDVLKKKSTTTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDD-VQWQTD 240
           K    KK  +  KD+ T      KN+ SDED SP   H ++     +DEDDDD V+WQTD
Sbjct: 181 K--AKKKALSNGKDSKT-----SKNHSSDEDISP--KHDENALEVDEDEDDDDGVEWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTE-ENKSSKKSSEHEKSAANGNKNGNGVSTHGAL 300
           TS EAA++R+ EQLSA TA+MVMLS  E E K + KS    KS  N  K  N       L
Sbjct: 241 TSREAAEKRMMEQLSAKTAEMVMLSAMEVEEKKAPKS----KSNGNVVKTENPPPQEKNL 300

Query: 301 IQEIKDYLKKGASTTDLKSFLESLTGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFLAV 360
           +Q++K+YLKKG+  + LKSF+ SL+   Q+I++AL  ALFDGVGKGF+KEV KKK +LA 
Sbjct: 301 VQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTKKKNYLAA 360

Query: 361 VAAV--EGSQIILLRAIESFC-LNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVA 420
            A +  +GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL+WY++G+ 
Sbjct: 361 AATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLDWYEKGLT 420

Query: 421 GANKSSQIWKYVKPFIEWLQNAESETEEE 445
           GA+KSS +WK VKPF+EWLQ+AESE+EEE
Sbjct: 421 GADKSSPVWKNVKPFVEWLQSAESESEEE 436

BLAST of Chy3G059920 vs. TAIR 10
Match: AT5G20920.1 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0

Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

BLAST of Chy3G059920 vs. TAIR 10
Match: AT5G20920.2 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0

Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 156 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 215

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 216 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 246

BLAST of Chy3G059920 vs. TAIR 10
Match: AT5G20920.3 (eukaryotic translation initiation factor 2 beta subunit )

HSP 1 Score: 53.9 bits (128), Expect = 3.8e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 0

Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQ-RLVVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P487241.6e-15968.30Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris OX=3885 GN=EIF5... [more]
P558761.4e-15568.28Eukaryotic translation initiation factor 5 OS=Zea mays OX=4577 GN=EIF5 PE=2 SV=1[more]
Q9C8F11.8e-15566.89Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana OX... [more]
Q9S8259.3e-15267.71Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana OX... [more]
P593255.3e-4633.11Eukaryotic translation initiation factor 5 OS=Mus musculus OX=10090 GN=Eif5 PE=1... [more]
Match NameE-valueIdentityDescription
A0A0A0LMB45.5e-24099.77W2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G310390 PE=3 SV=... [more]
A0A1S3BCI33.5e-23497.30eukaryotic translation initiation factor 5-like OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7VCS95.9e-23497.07Eukaryotic translation initiation factor 5-like OS=Cucumis melo var. makuwa OX=1... [more]
A0A6J1BQ302.4e-21991.29eukaryotic translation initiation factor 5-like OS=Momordica charantia OX=3673 G... [more]
A0A6J1HCN37.3e-21689.51probable eukaryotic translation initiation factor 5-1 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
XP_004150695.13.08e-30599.77eukaryotic translation initiation factor 5 [Cucumis sativus] >XP_011649412.1 euk... [more]
XP_008445359.11.28e-29797.30PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo] >XP_00... [more]
KAA0064817.12.59e-29797.07eukaryotic translation initiation factor 5-like [Cucumis melo var. makuwa][more]
XP_038884689.15.74e-29095.28eukaryotic translation initiation factor 5-like [Benincasa hispida][more]
XP_022131574.14.90e-27891.29eukaryotic translation initiation factor 5-like [Momordica charantia] >XP_022131... [more]
Match NameE-valueIdentityDescription
AT1G36730.11.3e-15666.89Translation initiation factor IF2/IF5 [more]
AT1G77840.16.6e-15367.71Translation initiation factor IF2/IF5 [more]
AT5G20920.13.8e-0729.03eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.23.8e-0729.03eukaryotic translation initiation factor 2 beta subunit [more]
AT5G20920.33.8e-0729.03eukaryotic translation initiation factor 2 beta subunit [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 159..179
NoneNo IPR availableGENE3D3.30.30.170coord: 2..104
e-value: 9.0E-43
score: 147.0
NoneNo IPR availableGENE3D2.20.25.350coord: 105..145
e-value: 1.6E-19
score: 71.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..223
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 266..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..235
NoneNo IPR availablePANTHERPTHR23001:SF28EUKARYOTIC TRANSLATION INITIATION FACTOR 5-1-RELATEDcoord: 1..444
NoneNo IPR availablePANTHERPTHR23001EUKARYOTIC TRANSLATION INITIATION FACTORcoord: 1..444
NoneNo IPR availableCDDcd11561W2_eIF5coord: 293..442
e-value: 1.23879E-35
score: 127.347
IPR003307W2 domainSMARTSM00515542_3coord: 353..440
e-value: 1.3E-30
score: 117.7
IPR003307W2 domainPFAMPF02020W2coord: 366..444
e-value: 1.1E-19
score: 70.4
IPR003307W2 domainPROSITEPS51363W2coord: 288..444
score: 24.083931
IPR002735Translation initiation factor IF2/IF5SMARTSM00653eIF2Bneu4coord: 15..129
e-value: 9.2E-63
score: 224.5
IPR002735Translation initiation factor IF2/IF5PFAMPF01873eIF-5_eIF-2Bcoord: 11..127
e-value: 6.1E-37
score: 126.1
IPR016021MIF4G-like domain superfamilyGENE3D1.25.40.180coord: 292..444
e-value: 3.0E-27
score: 97.4
IPR016189Translation initiation factor IF2/IF5, N-terminalSUPERFAMILY100966Translation initiation factor 2 beta, aIF2beta, N-terminal domaincoord: 10..101
IPR016190Translation initiation factor IF2/IF5, zinc-bindingSUPERFAMILY75689Zinc-binding domain of translation initiation factor 2 betacoord: 98..133
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 298..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G059920.1Chy3G059920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001732 formation of cytoplasmic translation initiation complex
biological_process GO:0001731 formation of translation preinitiation complex
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0006413 translational initiation
cellular_component GO:0005829 cytosol
molecular_function GO:0071074 eukaryotic initiation factor eIF2 binding
molecular_function GO:0005092 GDP-dissociation inhibitor activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0005515 protein binding