Chy3G059610 (gene) Cucumber (hystrix) v1

Overview
NameChy3G059610
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.6-like
LocationchrH03: 13318985 .. 13325156 (+)
RNA-Seq ExpressionChy3G059610
SyntenyChy3G059610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATGTATGTTCATCCCTAAATTCCTTTATGAAAGTCATGGTAGGGTCATAAAAATTTCAATAATACGTACCTTACTGAATGAAATTGGTTGGATATGTAGGAAGCCGTGAAGGAGGAGAGCGACGGGAGCATATGGGAAGGGTATGTGGATTGGCGGAAGAGGCCTGCAGTCAAAGGGCGGCATGGGGGAATGATAGCGGCTGGATTCGTATTAGGTTGGTGAATATATACATACATACATACATACATACATTTAAATAATAAGTAGTTAAATTTGGTATTAATTAATTATTATTGCAGGAGTTGAAGCGTTGGAGAATTTGGCGTTCTTGGCAAATGCAAGTAATTTGGTGATGTATTTGAGGAAATATATGGGATTTTCACCGGCGAAATCCGCAAACCATGTCACGACGTTTATGGGAACGGCGTTTCTTCTTGCACTTCTTGGTGGTTTCATATCGGATGCGTTTTTCACAACTTATTACGTCTTCCTTTTCTCCTCTTTCATTGAGTTTCTGGTTCGTCTTATTTAACTCCTCTTTATGTTTTTTTCTTTTCTTTTTAATCTCTATTTTAGTTAAAATAGTAGTGTTTTTATGTAGGTTAGTACATCGATTGGGTGAGAATTGGGTAGGAGATTCGAATCAAAACAAACAAACGTGGGTGTGTTATTGTTAATAGGGAATTAAACTGATGTTTTAATATGTTTCTTGTATCTTTTCGTATTAACTTGAATATATTTTTAGAACAAATGGTGATCAGAACCAAAATAAAAGAAAAACAAAAGAAGCCCTTTTTTTTTCTTTCTTTGGCCTATTTTCTTCTCTTTTATTTTAAAATTTTATTCTCTGCCATGATTTATGAGAACCCATGTGATCGGCCATGAAGATTGTTGGAATAATTGTATTTATTATTTCGAAATTTTCAGACTTTTAGTCGGCTTTGTTATTCAAAGTCCTTTATTTTTTTTATGTTTGAAAAGTCAAGTTAAGAATTTAGTAATATTTCAATTTACATGACAAAATCTATCAAATTTTTCAGTGCACACCTAAGTTCCTTTTTTGGTATAAATTTTTGTTTTATTTTTTATTTTTGAAAATTAAGCCTATAAACTTTCTTTATCTCTAAATTGTTTTAATTTACATTTTTTATTATTGTAAATCTGTTTTTTTTTAAAAAAAACTTAGGTAAGAATTAATTTTTTATAAATAGATTTAATTTTTAAAAATAAAAGACTTAAAAACAAATTTAGTAACTATTTTCTATTTTTATTTTTGAAAAGAGGAAGAAAGATGAAATAATATTTTTAGGTTTAATTTTCAAAAACCAATAACAAAAAAATGAAATGGTTACGTGGGGGTCTAAACAAGATGGTTTTTTTTTTCTTCCATTATCTCCAATCAATTTCTTCTTTCTTAGTTTTGTTATATTGAATTGAAAAAAATGAAATGAATAGAAGGGGAATATGTCCCACAACCAAAAAAACAACGTAGGCATCCGTTTTTGCCCCGAAGATAATGACACAAATCCCAACAATAAAATACCTTGTTATTATATATATATGTATGTATTTTATTAATATCATATTAAAGTTAGTAATAGTATGGGCGCTATCGTCAAGATATATACATATATTTTACGTCACACACATGAGAAAAACAATCTATTTTTATAATTAAAATATTGGTATCTTTGTCAAACTTAAATGTTAAAAAAAACTTAATTAATTTTGTTTTTTTTTTTTATATTCCAAAGCTAACTCAAACACACATTCAAAAGTTGGTAACTTTAAAGAATTTTCAACTAAATTCCTACTCAACAAGTTGATCAAGTTGAATGAGAGAAAAAGATAATACTATAAATCTTGACATATGTCTAAGATTCTAAGATTGTAATTTGGGAGTAATGAGAATAAAGTCTTATTGTCAAATATATACACAAATAGAAGATTATTGGTAAATATAAGTAAAAAAATAAACATATACACCAGTATGAAAAGTATTATTTATTGAGTTGATAAAAAGTAAAAAAGCAAATCCACGTTTTCATATAGCTTTAAAGATCTTTAAAAATTTAAATTATACCTTTGAAGAAAAAGTGATGAATGGGCAAAATTACAAAACTAATCCTTTGAATTTTTAAATTTACAAAATTATAAATTTAGTCTTCCGACTTTAACAATTGTATTAGAGATTATTTTTTTGAAAACTTGAAAGTTGTTTTATAGATACTAATAGATAAATTTTGTTTTAATATAGTTGAATGATTATATACTAGATGATGCATGGTTATGGAAGAGAATGATAAAAATATGAAAGAAAAAGAGAGAGAGAGGAATGGAAATAATAATAAAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGTTGAAGGGACCCAGAAAGGAGTGAATTAGTAACAGTGAGAATGGTGATGAGAGTGTGTGTTACGACATTGGAACTCATCCGTTTGCGTCAAACCAAATAAAGCTTTTTTTCTTTTGATCTCGTTTTCACCACCTCCACTAAACCAACTTCTTCCTTCCTCAACTTGTCCTTTTCCATTTCTTTTTCTCTTTTCCTTTTCATTGCAAGTAGACGACTAGCACAACCCTACATATCTCTTCTCTCTTTTAAATCCCTTCCAATCAACCTCTTTTTCTCCAACCCATCAAAATAATAATAATAATAATAATAAACTCGAGTCCATGATACAATCTTGCTGACACCCAATAGAAATATGTTATTGGACACTCTTTTTTTCTTTGTTAAAGAATGGTTTTTTTTTTTTTTCACTTGTGTGTAAATGAGGGAAACGTTTGAGAGTAGACGTAGTCTGAATTGCACCTAGTTATTGCTCTAAAAATAGAAAAAAATCTATTTTTATCTTTTATTTTGAGTCTAGGTTTCAATTGATTAGGTTTTAAGAAAGTTATAATTTGACCGTGTTTCAAATTTTACACTTTTGACTTTGATCGTTTGTTGTTTTTTTTAGTGCTAATGCCTATTAATTAGTTTAAAAGAATTATAATAGATTAAGTTTCAATTTTTTCATCAAGTAAAATTAAAGGATATTTTTACTTCATAATTATTTTAAATCAATTAATATACATTAACTCTAATAATTGAGAGTAAATATTTAATATTAAAAATAAGGTAAACTTGTAAATCTTAAAACCTAGCAAGGAATAAAATTGAAACAAAACTCAAATTTCAAAAGTAAGTAAAATTGTGATGTTTTAAAATTTAAATACTAAACTGAAGCAAAACTCAAAACTTAAAAAAGACTAAATGGTAACATTTTGAAATCTAGGACTAAAAAAAAAGGATAAAAACCTTTATACAATAAACATATTAAATAACAAAATGTGTGGAATAATATATGATGGGTTACTCGTATGAGATAACAAAATTCTCAAAACTGGAAAGACTCGGAGCATATATTCAGATTATACATCTACATCTAATTGCATCGTTTTGTAGAGAATTTTCAAAGTAACTTGATAATATAGTTTGACAGTTTTCAAAGTAATTTTATTTATTTTCATGACTTTCTTTCTCATCCATCAAAACTCTTTACAGAACTACACTCCAACCACTATTTTTTCCCCTTAATTTAGTTGCTTTTTATGTAATACGTCATTGTCTTTTCATCTACTTTCGGTCTGCTTGTTTTTGTTTTTTTGTGCTTCTATTACTATTAATGAATTGACAGTGGAATACAATGTAATCATGATAATATAGATGTGTCAATGTAGTTGAAATTCTCTAAAAACAATATCTTAGTGTGTATGCTTGTTAAAATACTTTGAATATGTTATATAGCTGTTTGAATGAACAAATAAGGAGTTTAGAGAAATGCATTATGTAAATTCTCTAACCCCAGTGACACTAAGTATTTTGTAACATTTTATCAAATAAAATTGTTTTTTCTTTTTCTCACGAAAGTAACTGATACACCAGTTAACGAATCACATATTTCTATGTGTTTGATTTTCTATTTTTCTTATTTGTTGATTTTGTAACCATGCTGATCGGTGCTAATATACACATGGAATAGGGGTTAGTGATGCTGACAGTGCAAGCAAAGGTGAGTTCATCATGCGAGGAGGGGAGCTCAAAGGGGTGCAAGGAAGTAGGGGGGAAAGAGATGGCGGTGCTGTTCATAGGGCTGTACTTGGTGGCGTTGGGCGTCGGTGGAATAAAGGGGTCACTGCCGGCGCACGGGGCAGAGCAGTTCGACGAAAGCACCCCACAAGGGAGGAAGAGGAGGTCTAGTTTCTTTAACTACTTTGTGTTTAGTCTGTCATGTGGAGCCCTTGTTGCAGTGACATTCGTGGTTTGGATTGAAGACAACTTGGGGTGGCAATGGGGTTTTGGAATCTCAACTATCAGCATTTTTCTATCTATTCCTTTGTTCTTTGCTGGTTCTCCTTTTTATCGTAACAAAATCCCGACTGGTAGTCCCCTTACCACCATTCTCAAGGTATTTTTCTACCCTTACAACTAATATGCATTTCATTCAAGTATATAATTAAATATAACAAAATATCGTCACATGAGTGGCTAATACTTTTTTTTAAAAAACATATTTTTTTATATTGTTTTTACTTTGTAATTAAAAGAGAATGCAAAGAAGATGAGTGTAGACTAGATTACAACAAAGTATTTGCTTTTTGAGTGTAGCTCAAAGTTTAATATCCTCATGCACTCGATTGAGAAAAGAAAAATATATATATTTATATTTTTAGAAGTATTAAAATATGTTTCTTTTAAGAAAAGTTTGCTATGTGTCATCGATGATTGAGTATTAGAGAGAATAGCGTAGAAAGGTAGATACACCTCGAGTTATACCAAAGTATTTTTTTTATAGGATAATTGCAATATGGATAACAATTCTATAACTAAAAATAAATTGTATATAATAACATTTTTAAAGCATTGCAAAATATAGAAAAATATATCCGTAATAGAATCTATTATTAATACAGTATTCCTATCAATAAAAAAAGTAGATCGACAGTAATAAACTCATATTATTAGCAACGTAGTTTATTACTGATAGACTCTAGAAGAGTTAGAATCTAAACTTTACTTATCTACAAATTCTTTTACATTATATTATATTTGTTAATGCTTTGAGACTTATTTACAATTGGCCCAAGATTAGTATAACATTTAATTATATGAATGATGATATAATTAGGTACTAGTAGCAGCAACATTGAACCGTAGGAGCAACAAAAAACCAGCAACAAACGCAGTGGCAAGCCTTGCAAGAAGCCCATCAACTACCTCAACCCCAAAGCAACTAATCAATAAAGACCTAATAGAAAACAATGCAACTACTCCAACTCAAAGCCTCAAATTCCTAAACAAAGCAGTACAAAACCCACCTTTCCACCCATCACTAAAATGCACAACCCAACAACTTGAAGACGTAAAAATAGTCATAAAAGTCCTCCCAATCTTTGCATGCACCATAATACTCAACTCATGCTTAGCTCAACTCTCCACTTTCTCCATAGAACAAGCCGCCACAATGAACACCAAAATCTACTCCCTCAAAATCCCCCCAGCCTCCCTCCCAGTCTTCCCTGTCCTATTCATCATCATCCTCGCCCCTCTCTACGACCATCTCATCATCCCCTTCGCTCGATCCCTCACACGCACCGAAGCCGGCATCACCCACCTCCAACGCATTGGTGTCGGCCTCCTCCTCTCTGTCCTTGCCATGGCTGTCGCTGCACTGGTCGAGACCAAACGTAAGGCCATGGCTGATGCCAACCCGGATGTGCACGGCCCGCTTCCCATTACGTTCCTTTGGATTGCATTCCAGTACTTGTTTTTGGGGTCGGCGGATTTGTTTACGTTGGCGGGTTTGTTGGAGTTTTTCTTCACTGAGGCTCCGGCTACTATGAGGTCTTTGGCTACGGCTCTTTCATGGGCTTCGTTGGCTGTTGGGTATTATTTTAGTTCGGTCATTGTATCGGTTGTGAACCATGCGAGTGCCAAAGCTTGGCATAGGCCTTGGCTGTCTGGCCACAACATTAATCACTATCGTCTTTCTAACTTCTATTGGGTTATGTGTGTTCTTAGTGGCCTCAATTTCTTGCACTACCTTTTTTGGGCTCTTAAGTATAAATATCGATCCACCCAAGGGAACAAATGA

mRNA sequence

ATGGAAGCCGTGAAGGAGGAGAGCGACGGGAGCATATGGGAAGGGTATGTGGATTGGCGGAAGAGGCCTGCAGTCAAAGGGCGGCATGGGGGAATGATAGCGGCTGGATTCGTATTAGGAGTTGAAGCGTTGGAGAATTTGGCGTTCTTGGCAAATGCAAGTAATTTGGTGATGTATTTGAGGAAATATATGGGATTTTCACCGGCGAAATCCGCAAACCATGTCACGACGTTTATGGGAACGGCGTTTCTTCTTGCACTTCTTGGTGGTTTCATATCGGATGCGTTTTTCACAACTTATTACGTCTTCCTTTTCTCCTCTTTCATTGAGTTTCTGGGGTTAGTGATGCTGACAGTGCAAGCAAAGGTGAGTTCATCATGCGAGGAGGGGAGCTCAAAGGGGTGCAAGGAAGTAGGGGGGAAAGAGATGGCGGTGCTGTTCATAGGGCTGTACTTGGTGGCGTTGGGCGTCGGTGGAATAAAGGGGTCACTGCCGGCGCACGGGGCAGAGCAGTTCGACGAAAGCACCCCACAAGGGAGGAAGAGGAGGTCTAGTTTCTTTAACTACTTTGTGTTTAGTCTGTCATGTGGAGCCCTTGTTGCAGTGACATTCGTGGTTTGGATTGAAGACAACTTGGGGTGGCAATGGGGTTTTGGAATCTCAACTATCAGCATTTTTCTATCTATTCCTTTGTTCTTTGCTGGTTCTCCTTTTTATCGTAACAAAATCCCGACTGGTAGTCCCCTTACCACCATTCTCAAGGTACTAGTAGCAGCAACATTGAACCGTAGGAGCAACAAAAAACCAGCAACAAACGCAGTGGCAAGCCTTGCAAGAAGCCCATCAACTACCTCAACCCCAAAGCAACTAATCAATAAAGACCTAATAGAAAACAATGCAACTACTCCAACTCAAAGCCTCAAATTCCTAAACAAAGCAGTACAAAACCCACCTTTCCACCCATCACTAAAATGCACAACCCAACAACTTGAAGACGTAAAAATAGTCATAAAAGTCCTCCCAATCTTTGCATGCACCATAATACTCAACTCATGCTTAGCTCAACTCTCCACTTTCTCCATAGAACAAGCCGCCACAATGAACACCAAAATCTACTCCCTCAAAATCCCCCCAGCCTCCCTCCCAGTCTTCCCTGTCCTATTCATCATCATCCTCGCCCCTCTCTACGACCATCTCATCATCCCCTTCGCTCGATCCCTCACACGCACCGAAGCCGGCATCACCCACCTCCAACGCATTGGTGTCGGCCTCCTCCTCTCTGTCCTTGCCATGGCTGTCGCTGCACTGGTCGAGACCAAACGTAAGGCCATGGCTGATGCCAACCCGGATGTGCACGGCCCGCTTCCCATTACGTTCCTTTGGATTGCATTCCAGTACTTGTTTTTGGGGTCGGCGGATTTGTTTACGTTGGCGGGTTTGTTGGAGTTTTTCTTCACTGAGGCTCCGGCTACTATGAGGTCTTTGGCTACGGCTCTTTCATGGGCTTCGTTGGCTGTTGGGTATTATTTTAGTTCGGTCATTGTATCGGTTGTGAACCATGCGAGTGCCAAAGCTTGGCATAGGCCTTGGCTGTCTGGCCACAACATTAATCACTATCGTCTTTCTAACTTCTATTGGGTTATGTGTGTTCTTAGTGGCCTCAATTTCTTGCACTACCTTTTTTGGGCTCTTAAGTATAAATATCGATCCACCCAAGGGAACAAATGA

Coding sequence (CDS)

ATGGAAGCCGTGAAGGAGGAGAGCGACGGGAGCATATGGGAAGGGTATGTGGATTGGCGGAAGAGGCCTGCAGTCAAAGGGCGGCATGGGGGAATGATAGCGGCTGGATTCGTATTAGGAGTTGAAGCGTTGGAGAATTTGGCGTTCTTGGCAAATGCAAGTAATTTGGTGATGTATTTGAGGAAATATATGGGATTTTCACCGGCGAAATCCGCAAACCATGTCACGACGTTTATGGGAACGGCGTTTCTTCTTGCACTTCTTGGTGGTTTCATATCGGATGCGTTTTTCACAACTTATTACGTCTTCCTTTTCTCCTCTTTCATTGAGTTTCTGGGGTTAGTGATGCTGACAGTGCAAGCAAAGGTGAGTTCATCATGCGAGGAGGGGAGCTCAAAGGGGTGCAAGGAAGTAGGGGGGAAAGAGATGGCGGTGCTGTTCATAGGGCTGTACTTGGTGGCGTTGGGCGTCGGTGGAATAAAGGGGTCACTGCCGGCGCACGGGGCAGAGCAGTTCGACGAAAGCACCCCACAAGGGAGGAAGAGGAGGTCTAGTTTCTTTAACTACTTTGTGTTTAGTCTGTCATGTGGAGCCCTTGTTGCAGTGACATTCGTGGTTTGGATTGAAGACAACTTGGGGTGGCAATGGGGTTTTGGAATCTCAACTATCAGCATTTTTCTATCTATTCCTTTGTTCTTTGCTGGTTCTCCTTTTTATCGTAACAAAATCCCGACTGGTAGTCCCCTTACCACCATTCTCAAGGTACTAGTAGCAGCAACATTGAACCGTAGGAGCAACAAAAAACCAGCAACAAACGCAGTGGCAAGCCTTGCAAGAAGCCCATCAACTACCTCAACCCCAAAGCAACTAATCAATAAAGACCTAATAGAAAACAATGCAACTACTCCAACTCAAAGCCTCAAATTCCTAAACAAAGCAGTACAAAACCCACCTTTCCACCCATCACTAAAATGCACAACCCAACAACTTGAAGACGTAAAAATAGTCATAAAAGTCCTCCCAATCTTTGCATGCACCATAATACTCAACTCATGCTTAGCTCAACTCTCCACTTTCTCCATAGAACAAGCCGCCACAATGAACACCAAAATCTACTCCCTCAAAATCCCCCCAGCCTCCCTCCCAGTCTTCCCTGTCCTATTCATCATCATCCTCGCCCCTCTCTACGACCATCTCATCATCCCCTTCGCTCGATCCCTCACACGCACCGAAGCCGGCATCACCCACCTCCAACGCATTGGTGTCGGCCTCCTCCTCTCTGTCCTTGCCATGGCTGTCGCTGCACTGGTCGAGACCAAACGTAAGGCCATGGCTGATGCCAACCCGGATGTGCACGGCCCGCTTCCCATTACGTTCCTTTGGATTGCATTCCAGTACTTGTTTTTGGGGTCGGCGGATTTGTTTACGTTGGCGGGTTTGTTGGAGTTTTTCTTCACTGAGGCTCCGGCTACTATGAGGTCTTTGGCTACGGCTCTTTCATGGGCTTCGTTGGCTGTTGGGTATTATTTTAGTTCGGTCATTGTATCGGTTGTGAACCATGCGAGTGCCAAAGCTTGGCATAGGCCTTGGCTGTCTGGCCACAACATTAATCACTATCGTCTTTCTAACTTCTATTGGGTTATGTGTGTTCTTAGTGGCCTCAATTTCTTGCACTACCTTTTTTGGGCTCTTAAGTATAAATATCGATCCACCCAAGGGAACAAATGA

Protein sequence

MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLRKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQAKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNATTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRIGVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK*
Homology
BLAST of Chy3G059610 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.6e-212
Identity = 395/582 (67.87%), Postives = 474/582 (81.44%), Query Frame = 0

Query: 7   ESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLRKYMGF 66
           E + S WEGY DWR R AVKGRHGGM+AA FVL VE LENLA+LANASNLV+YLR+YM  
Sbjct: 4   EEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHM 63

Query: 67  SPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQAKVSS- 126
           SP+KSAN VT FMGTAFLLALLGGF+SDAFF+T+ +FL S+ IEFLGL++LT+QA+  S 
Sbjct: 64  SPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSL 123

Query: 127 ---SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKR 186
              SC+   S  C+EV G + A+LF+GLYLVALGVGGIKGSL +HGAEQFDESTP+GRK+
Sbjct: 124 MPPSCD---SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQ 183

Query: 187 RSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNK 246
           RS+FFNYFVF L+CGALVAVTFVVW+EDN GW+WGFG+STI+IF+SI +F +GS FYRNK
Sbjct: 184 RSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNK 243

Query: 247 IPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPS---TTSTPKQLINKDLIE-- 306
           IP GSPLTTILKVL+AA++   S+   ++NAVAS++ SPS    +   K++ ++  +E  
Sbjct: 244 IPCGSPLTTILKVLLAASVKCCSSGS-SSNAVASMSVSPSNHCVSKGKKEVESQGELEKP 303

Query: 307 --NNATTP----TQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNS 366
               A  P    T SLK LN A    P H  L+CT QQ+EDVKIV+K+LPIFACTI+LN 
Sbjct: 304 RQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNC 363

Query: 367 CLAQLSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTE 426
           CLAQLSTFS++QAA+MNTKI SLKIPPASLP+FPV+FI+ILAP+YDHLIIPFAR  T+TE
Sbjct: 364 CLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTE 423

Query: 427 AGITHLQRIGVGLLLSVLAMAVAALVETKRKAMADANP--DVHGPLPITFLWIAFQYLFL 486
            G+THLQRIGVGL+LS+LAMAVAALVE KRK +A  +   D    LP+TFLWIA QYLFL
Sbjct: 424 TGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFL 483

Query: 487 GSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRP 546
           GSADLFTLAGLLE+FFTEAP++MRSLAT+LSWASLA+GYY SSVIVS+VN  +  + + P
Sbjct: 484 GSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTP 543

Query: 547 WLSGHNINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRST 572
           WL G +IN Y+L  FYW+MCVLS  NFLHYLFWA++YKYRST
Sbjct: 544 WLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRST 581

BLAST of Chy3G059610 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.7e-199
Identity = 368/574 (64.11%), Postives = 447/574 (77.87%), Query Frame = 0

Query: 4   VKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLRKY 63
           V+   D S WEGY DWR + A++GRHGGM+AA FVL VE LENLAFLANASNLV+YL+ +
Sbjct: 3   VEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNF 62

Query: 64  MGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQAKV 123
           M  S A+S++ VTTFM TAFLLALLGGF++DAFF+T+ +FL S+ IEFLGL++LT+QA+ 
Sbjct: 63  MHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARR 122

Query: 124 SS----SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQG 183
            S     C+  ++  C+ VGG + A LF+GLYLV+LG+GGIKGSLP+HGAEQFDE TP+G
Sbjct: 123 PSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKG 182

Query: 184 RKRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFY 243
           RK+RS+FFNY+VF LSCGALVAVTFVVWIEDN GW+WGFG+STISIFLSI +F  GS FY
Sbjct: 183 RKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFY 242

Query: 244 RNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENN 303
           +NKIP GSPLTTI KVL+AA++   S+K  + +  +   +S     TP Q +        
Sbjct: 243 KNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL-------- 302

Query: 304 ATTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTF 363
               T SL  LNKA++    H  L+CT QQ+EDVKIV+K+LPIF CTI+LN CLAQLST+
Sbjct: 303 ----TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTY 362

Query: 364 SIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQR 423
           S+ QAATMN KI +  +P ASLPVFPV+F++ILAP YDHLIIPFAR +T++E GITHLQR
Sbjct: 363 SVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQR 422

Query: 424 IGVGLLLSVLAMAVAALVETKRKAMADANP--DVHGPLPITFLWIAFQYLFLGSADLFTL 483
           IGVGL+LS++AMAVAALVE KRK +A      D    LPITFLWIA QYLFLGSADLFTL
Sbjct: 423 IGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTL 482

Query: 484 AGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNIN 543
           AGLLEFFFTEAP++MRSLAT+LSWASLA+GYY SSV+V +VN  +  A   PWL G  +N
Sbjct: 483 AGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLN 542

Query: 544 HYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRST 572
             RL  FYW+MCVLS +NFLHYLFWA +YKY ST
Sbjct: 543 RNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Chy3G059610 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 661.4 bits (1705), Expect = 9.5e-189
Identity = 352/580 (60.69%), Postives = 434/580 (74.83%), Query Frame = 0

Query: 2   EAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLR 61
           E + E +  S W GYVDWR RPA++GRHGGM+AA FVL VE LENLAFLANASNLV+YL 
Sbjct: 30  EQMDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS 89

Query: 62  KYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQA 121
             MGFSP+ +AN VT FMGTAF LALLGGF++DAFFTT++++L S+ IEFLGL++LTVQA
Sbjct: 90  TKMGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQA 149

Query: 122 KVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRK 181
                  E S++    V       LF+GLYLVALGVGGIKGSLP HGAEQFDE T  GR+
Sbjct: 150 ------HEHSTEPWSRV------FLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRR 209

Query: 182 RRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRN 241
           +RS FFNYF+FSLSCGAL+AVT VVW+EDN GW +GFG+ST +I +S+P+F AGS  YR 
Sbjct: 210 QRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRL 269

Query: 242 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNAT 301
           K+P+GSP+TT+ KVL AA   +   ++ +   V    R+    S  KQ  + D       
Sbjct: 270 KVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVTKQNCDGD------- 329

Query: 302 TPTQSLKFLNKAVQNPPFHP-SLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 361
                  FL + V+     P  L+CT +Q++DVKIVIK+LPIF  TI+LN CLAQLSTFS
Sbjct: 330 -DGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFS 389

Query: 362 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 421
           ++QA+TMNTK+ S  +PPA+LPVFPV+F++ILAP Y+HL++P AR  T+TE GITHLQRI
Sbjct: 390 VQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRI 449

Query: 422 GVGLLLSVLAMAVAALVETKRKAMA-------DANPDVHGPLPITFLWIAFQYLFLGSAD 481
           G GL+LS++AMAVAALVETKRK +        +++     PLPITFLW+A QY+FLGSAD
Sbjct: 450 GTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSAD 509

Query: 482 LFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSG 541
           LFTLAG++EFFFTEAP+TMRSLAT+LSWASLA+GYYFSSV+VS VN  +    H PWL G
Sbjct: 510 LFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLG 569

Query: 542 HNINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQG 574
            N+N Y L  FYW+MCVLSG+NFLHYLFWA +Y YRS QG
Sbjct: 570 ENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQG 589

BLAST of Chy3G059610 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.2e-103
Identity = 231/590 (39.15%), Postives = 348/590 (58.98%), Query Frame = 0

Query: 5   KEESDGSIW----EGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 64
           +E S+ + W    E  VDWR RP+   +HGGM AA FVLG++A E +   A  +NL+ Y+
Sbjct: 14  EEVSNENNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYV 73

Query: 65  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 124
              M F  +K+AN VT F+GT F+ ALLGG++SDAF  +++  +   F+E  G ++L+VQ
Sbjct: 74  INEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQ 133

Query: 125 AKVSS----SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDEST 184
           A +       C     + C+E  G +  + F+ LYLVALG G +K ++ AHGA+QF +S 
Sbjct: 134 AHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSH 193

Query: 185 PQGRKRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGS 244
           P+  KR SS+FN   F+ S G L+A+T +VW++ + G   GFG+S  ++ + I    +G+
Sbjct: 194 PKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGT 253

Query: 245 PFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQL-----I 304
            ++RNK P  S  T I  V+VAA L R+                 ++ S P+ L     +
Sbjct: 254 MYFRNKRPRRSIFTPIAHVIVAAILKRKL----------------ASPSDPRMLHGDHHV 313

Query: 305 NKDLIENNATTPTQSLKFLNKAV-----QNPPFHPSLKCTTQQLEDVKIVIKVLPIFACT 364
             D++ ++    T   +FL+KA       N    P   CT  Q+E VK +I ++PIFA T
Sbjct: 314 ANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFAST 373

Query: 365 IILNSCLAQLSTFSIEQAATMNTKI-YSLKIPPASLPVFPVLFIIILAPLYDHLIIPFAR 424
           I+ N+ LAQL TFS++Q ++MNT++  S  IPPASL   P + +I L PLYD  ++PFAR
Sbjct: 374 IVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFAR 433

Query: 425 SLTRTEAGITHLQRIGVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQ 484
            LT   +GI  L RIG+GL LS  +M  AA++E KR+  +  +  +     ++  WI  Q
Sbjct: 434 KLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-----LSIFWITPQ 493

Query: 485 YLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNH-ASAK 544
           +L  G +++FT  GL+EFF+ ++   M S   AL++ S + G+YFSSV+VSVVN   S  
Sbjct: 494 FLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTS 553

Query: 545 AWHRPWLSGHNINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGN 575
              + WL  +++N  RL  FYW++ VLS LNFL YLFW+ ++  +S++ N
Sbjct: 554 VDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS-RWNIKSSRRN 581

BLAST of Chy3G059610 vs. ExPASy Swiss-Prot
Match: Q9LSF0 (Protein NRT1/ PTR FAMILY 4.1 OS=Arabidopsis thaliana OX=3702 GN=NPF4.1 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 4.8e-100
Identity = 225/555 (40.54%), Postives = 331/555 (59.64%), Query Frame = 0

Query: 14  EGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLRKYMGFSPAKSAN 73
           E + DWR + A+ G+HGG+ AA     VE +EN+ FLA ++N +MY  K M +S  K+A 
Sbjct: 7   EKFEDWRGKEAISGKHGGIKAAFIACVVETMENMVFLACSTNFMMYFTKSMNYSTPKAAT 66

Query: 74  HVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQAKVSSSCEEGSSK 133
            VT F+GT+FLL + GGF++D+F T +  F+    IE LGL+MLT+QA ++    +G   
Sbjct: 67  MVTNFVGTSFLLTIFGGFVADSFLTRFAAFVLFGSIELLGLIMLTLQAHITKLQPQGG-- 126

Query: 134 GCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKRRSSFFNYFVFS 193
             K+    +  VLF GLY +A+GVGG+KGSLPAHG +Q      + ++  S FFN++ FS
Sbjct: 127 --KKPSTPQSTVLFTGLYAIAIGVGGVKGSLPAHGGDQIG---TRNQRLISGFFNWYFFS 186

Query: 194 LSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNKIPTGSPLTTIL 253
           +  G  +AVT +VWIE+N+GW   F IST  +  +I +F AG P YR K P GSPLT I+
Sbjct: 187 VCLGGFLAVTLMVWIEENIGWSSSFTISTAVLASAIFVFVAGCPMYRFKRPAGSPLTRIV 246

Query: 254 KVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNATTPTQSLKFLNKA 313
            V V+A  NR                  +   T  +++ ++    + +      KFLNKA
Sbjct: 247 NVFVSAARNR------------------NRFVTDAEVVTQNHNSTDKSIHHNKFKFLNKA 306

Query: 314 VQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFSIEQAATMNTKI-Y 373
             N       K +  Q+E+ +  + +LPIF  TII+N C+AQ+ TFS++Q    N K+  
Sbjct: 307 KLNN------KISATQVEETRTFLALLPIFGSTIIMNCCVAQMGTFSVQQGMVTNRKLSR 366

Query: 374 SLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGIT-HLQRIGVGLLLSVLAM 433
           S +IP ASL   P+L ++    LY+   +   R L+ +E   + +L+RIG GL L+ ++M
Sbjct: 367 SFEIPVASLNAIPLLCMLSSLALYE---LFGKRILSNSERSSSFNLKRIGYGLALTSISM 426

Query: 434 AVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPAT 493
           AVAA+VE KRK  A     VH  + I+  W+  Q++ L  +D+ T+ G+LEFFF E+PA+
Sbjct: 427 AVAAIVEVKRKHEA-----VHNNIKISVFWLELQFVMLSLSDMLTVGGMLEFFFRESPAS 486

Query: 494 MRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYRLSNFYWVMCVL 553
           MRS++TAL W S A+G++ SSV+V VVN  +       WL   ++N  RL  FY V+CVL
Sbjct: 487 MRSMSTALGWCSTAMGFFLSSVLVEVVNGITG------WLR-DDLNESRLELFYLVLCVL 515

Query: 554 SGLNFLHYLFWALKY 567
           + LN  +Y+FWA +Y
Sbjct: 547 NTLNLFNYIFWAKRY 515

BLAST of Chy3G059610 vs. ExPASy TrEMBL
Match: A0A1P8SN46 (NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 563/575 (97.91%), Postives = 567/575 (98.61%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           MEAVKEESDGSIWEGYVDWRKRPA KGRHGGMIAAGFVLGVE LENLAFLANASNLVMYL
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAAKGRHGGMIAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYMGFSPAKSANHVTTFMGTAFLLALLGGF+SDA FTTYYVF+FSSFIEFLGLVMLTVQ
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAIFTTYYVFIFSSFIEFLGLVMLTVQ 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR
Sbjct: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           KRRSSFFNYFVFSLSCGAL+AVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL ENN 
Sbjct: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLTENNV 300

Query: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360
           TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS
Sbjct: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360

Query: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420
           IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI
Sbjct: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420

Query: 421 GVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480
           GVGLLLSVLAMAVAALVETKRKA+ADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYR 540
           LEFFFTEAP TMRSLATALSWASLAVGYY SSVIVSVVNH SAKAWHRPWLSGHNINHYR
Sbjct: 481 LEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYR 540

Query: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 576
           LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK
Sbjct: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575

BLAST of Chy3G059610 vs. ExPASy TrEMBL
Match: A0A5D3BLL0 (Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00950 PE=3 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 8.6e-310
Identity = 552/575 (96.00%), Postives = 562/575 (97.74%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGM+AAGFVLGVE LENLAFLANASNLVMYL
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYMGFSPAKSANHVTTFMGTAFLLALLGGF+SDAFFTTYYVFLFSSF+EFLGLVMLTVQ
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQ 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AKVSSSCE G +KGCKEVGGKE+AVLFIGLYLVALGVGGIKGSLPAHGAEQFDEST QGR
Sbjct: 121 AKVSSSCEAG-TKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           KRRSSFFNYFVFSLSCGALVAVTFVVW+EDNLGWQWGFGISTIS FLSIPLFFAGSPFYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPK LINKDL E+N 
Sbjct: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDLTESNT 300

Query: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360
           TTPTQSLKFLNKAVQNPPFHPS+KCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS
Sbjct: 301 TTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360

Query: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420
           IEQAATMNTKI SLKIPPASLPVFPVLFII+LAPLYDHLIIPFARSLTRTEAGITHLQRI
Sbjct: 361 IEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGITHLQRI 420

Query: 421 GVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480
           GVGLLLSVLAMAVAALVETKRKA+ADANPDV GPLPITFLWIAFQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYR 540
           LEFFFTEAPATMRSLATALSWASLAVGYY SSVIVSVVNH SAKAWHRPWLSGHNINHYR
Sbjct: 481 LEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYR 540

Query: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 576
           LSNFYWVMCVLSG NFLHYLFWALKYKYRSTQGNK
Sbjct: 541 LSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Chy3G059610 vs. ExPASy TrEMBL
Match: A0A1S3C3A1 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 8.6e-310
Identity = 552/575 (96.00%), Postives = 562/575 (97.74%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGM+AAGFVLGVE LENLAFLANASNLVMYL
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYMGFSPAKSANHVTTFMGTAFLLALLGGF+SDAFFTTYYVFLFSSF+EFLGLVMLTVQ
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQ 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AKVSSSCE G +KGCKEVGGKE+AVLFIGLYLVALGVGGIKGSLPAHGAEQFDEST QGR
Sbjct: 121 AKVSSSCEAG-TKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           KRRSSFFNYFVFSLSCGALVAVTFVVW+EDNLGWQWGFGISTIS FLSIPLFFAGSPFYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPK LINKDL E+N 
Sbjct: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDLTESNT 300

Query: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360
           TTPTQSLKFLNKAVQNPPFHPS+KCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS
Sbjct: 301 TTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360

Query: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420
           IEQAATMNTKI SLKIPPASLPVFPVLFII+LAPLYDHLIIPFARSLTRTEAGITHLQRI
Sbjct: 361 IEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGITHLQRI 420

Query: 421 GVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480
           GVGLLLSVLAMAVAALVETKRKA+ADANPDV GPLPITFLWIAFQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYR 540
           LEFFFTEAPATMRSLATALSWASLAVGYY SSVIVSVVNH SAKAWHRPWLSGHNINHYR
Sbjct: 481 LEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYR 540

Query: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 576
           LSNFYWVMCVLSG NFLHYLFWALKYKYRSTQGNK
Sbjct: 541 LSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Chy3G059610 vs. ExPASy TrEMBL
Match: A0A0A0KHD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G404120 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 5.7e-253
Identity = 453/460 (98.48%), Postives = 455/460 (98.91%), Query Frame = 0

Query: 116 MLTVQAKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 175
           MLTVQAKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES
Sbjct: 1   MLTVQAKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDES 60

Query: 176 TPQGRKRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAG 235
           TPQGRKRRSSFFNYFVFSLSCGAL+AVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAG
Sbjct: 61  TPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAG 120

Query: 236 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL 295
           SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL
Sbjct: 121 SPFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL 180

Query: 296 IENNATTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 355
            ENN TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ
Sbjct: 181 TENNVTTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQ 240

Query: 356 LSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGIT 415
           LSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGIT
Sbjct: 241 LSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGIT 300

Query: 416 HLQRIGVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLF 475
           HLQRIGVGLLLSVLAMAVAALVETKRKA+ADANPDVHGPLPITFLWIAFQYLFLGSADLF
Sbjct: 301 HLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLF 360

Query: 476 TLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHN 535
           TLAGLLEFFFTEAP TMRSLATALSWASLAVGYY SSVIVSVVNH SAKAWHRPWLSGHN
Sbjct: 361 TLAGLLEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHN 420

Query: 536 INHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 576
           INHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK
Sbjct: 421 INHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 460

BLAST of Chy3G059610 vs. ExPASy TrEMBL
Match: A0A6J1G2M6 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 4.3e-245
Identity = 441/575 (76.70%), Postives = 498/575 (86.61%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           ME VKEE DG +WEGYVDWR+RPAV+GRHGGM AAGFVLGVE LENLAFLANASNLVM+L
Sbjct: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYM FSP +SANHVT FMGTAFLLALLGGF+SDAFFTTYYVFLFSS +EFLGLV+LTV+
Sbjct: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AK +++                 AV+F+GLYLVA+GVGGIKGSLP HGAEQF+E T +GR
Sbjct: 121 AKAATAA--------------SAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           + RSSFFNYFVFSLSCGA+VAVT VVW+EDNLGW+WGFGISTI+I +SIPLF AGS FYR
Sbjct: 181 RGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIP G+PLTTILKVLVAATLN RS  K A+NAVAS+ RSPSTT TPK         +  
Sbjct: 241 NKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTT-TPKPTKEP---THTT 300

Query: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360
             PTQ+LKFLNKA+Q PPFHPSL CTTQQLEDVK+V+K+LPIFACTIILNSCLAQLSTFS
Sbjct: 301 PPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFS 360

Query: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420
           +EQA+TMNTKI S+K+PPASLP+FP+LFII+LAPLY+HLIIPFAR+LTRTE+GITHLQRI
Sbjct: 361 VEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRI 420

Query: 421 GVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480
           GVGLLLS++AMA+AAL+E KRKA+ADA+P  H PLPITFLWI FQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYR 540
           LEFFFTEAP+TMRSLATALSWASLAVGYY SSVIVS+VN  +AK+ H PWLS  +INHY 
Sbjct: 481 LEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYD 540

Query: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 576
           L  FYW+MC LS LNFLH+LFWA+KYKYRST  +K
Sbjct: 541 LHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Chy3G059610 vs. NCBI nr
Match: XP_011657481.1 (protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >APY23675.1 NRT1.2-like nitrate transporter [Cucumis sativus] >KAE8647291.1 hypothetical protein Csa_004297 [Cucumis sativus])

HSP 1 Score: 1092 bits (2825), Expect = 0.0
Identity = 563/575 (97.91%), Postives = 567/575 (98.61%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           MEAVKEESDGSIWEGYVDWRKRPA KGRHGGMIAAGFVLGVE LENLAFLANASNLVMYL
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAAKGRHGGMIAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYMGFSPAKSANHVTTFMGTAFLLALLGGF+SDA FTTYYVF+FSSFIEFLGLVMLTVQ
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAIFTTYYVFIFSSFIEFLGLVMLTVQ 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR
Sbjct: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           KRRSSFFNYFVFSLSCGAL+AVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALIAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDL ENN 
Sbjct: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLTENNV 300

Query: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360
           TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS
Sbjct: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360

Query: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420
           IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI
Sbjct: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420

Query: 421 GVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480
           GVGLLLSVLAMAVAALVETKRKA+ADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSVLAMAVAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYR 540
           LEFFFTEAP TMRSLATALSWASLAVGYY SSVIVSVVNH SAKAWHRPWLSGHNINHYR
Sbjct: 481 LEFFFTEAPTTMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYR 540

Query: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575
           LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK
Sbjct: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575

BLAST of Chy3G059610 vs. NCBI nr
Match: XP_008456458.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa] >TYK00047.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1067 bits (2759), Expect = 0.0
Identity = 552/575 (96.00%), Postives = 562/575 (97.74%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGM+AAGFVLGVE LENLAFLANASNLVMYL
Sbjct: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMLAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYMGFSPAKSANHVTTFMGTAFLLALLGGF+SDAFFTTYYVFLFSSF+EFLGLVMLTVQ
Sbjct: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSFLEFLGLVMLTVQ 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AKVSSSCE G+ KGCKEVGGKE+AVLFIGLYLVALGVGGIKGSLPAHGAEQFDEST QGR
Sbjct: 121 AKVSSSCEAGT-KGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTTQGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           KRRSSFFNYFVFSLSCGALVAVTFVVW+EDNLGWQWGFGISTIS FLSIPLFFAGSPFYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFFAGSPFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPK LINKDL E+N 
Sbjct: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKPLINKDLTESNT 300

Query: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360
           TTPTQSLKFLNKAVQNPPFHPS+KCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS
Sbjct: 301 TTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360

Query: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420
           IEQAATMNTKI SLKIPPASLPVFPVLFII+LAPLYDHLIIPFARSLTRTEAGITHLQRI
Sbjct: 361 IEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFARSLTRTEAGITHLQRI 420

Query: 421 GVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480
           GVGLLLSVLAMAVAALVETKRKA+ADANPDV GPLPITFLWIAFQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYR 540
           LEFFFTEAPATMRSLATALSWASLAVGYY SSVIVSVVNH SAKAWHRPWLSGHNINHYR
Sbjct: 481 LEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYR 540

Query: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575
           LSNFYWVMCVLSG NFLHYLFWALKYKYRSTQGNK
Sbjct: 541 LSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Chy3G059610 vs. NCBI nr
Match: XP_038886228.1 (protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida])

HSP 1 Score: 1015 bits (2624), Expect = 0.0
Identity = 526/575 (91.48%), Postives = 548/575 (95.30%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           ME VKEESDGSIWEGYVDWRKR AV+GRHGGM+AAGFVLGVE LENLAFLANASNLVMYL
Sbjct: 1   MEIVKEESDGSIWEGYVDWRKRAAVRGRHGGMMAAGFVLGVEVLENLAFLANASNLVMYL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYM FSPAKSANHVTTFMGTAFLLALLGGF+SDAFFTTYYVFLFSS +EFLGLVMLTVQ
Sbjct: 61  RKYMEFSPAKSANHVTTFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVMLTVQ 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AKVSSSCEEG+SKGCKEVGG+E AVLFIGLYLVALGVGGIKGSLPAHGAEQFDE +PQGR
Sbjct: 121 AKVSSSCEEGNSKGCKEVGGREAAVLFIGLYLVALGVGGIKGSLPAHGAEQFDEGSPQGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           KRRSSFFNYFVFSLSCGAL+AVTFVVW+EDNLGWQWGFGISTISIFLSIPLF AGSPFYR
Sbjct: 181 KRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLFLAGSPFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIPTGSPLTTILKVLVAA LNR S+ KPATNAVASLARSPST ST KQLINK+L +NN 
Sbjct: 241 NKIPTGSPLTTILKVLVAAALNRGSSNKPATNAVASLARSPST-STAKQLINKELTQNNT 300

Query: 301 TTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 360
           T PTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIV+KVLPIFACTIILNSCLAQLSTFS
Sbjct: 301 TNPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVLKVLPIFACTIILNSCLAQLSTFS 360

Query: 361 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 420
           IEQA+TMNTKI SLK+PPASLP+FPVLFII+LAPLYDHLIIPFAR+LTRTEAGITHLQRI
Sbjct: 361 IEQASTMNTKISSLKVPPASLPIFPVLFIILLAPLYDHLIIPFARTLTRTEAGITHLQRI 420

Query: 421 GVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGL 480
           GVGLLLSVLAMAVAALVETKRKA+A A+PD H PLPITFLWIAFQYLFLGSADLFTLAGL
Sbjct: 421 GVGLLLSVLAMAVAALVETKRKAVAGAHPDAH-PLPITFLWIAFQYLFLGSADLFTLAGL 480

Query: 481 LEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYR 540
           LEFFFTEAPATMRSLATALSWASLAVGYY SSVIVSVVN  +AKA H PWLSGHNINHYR
Sbjct: 481 LEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKASHTPWLSGHNINHYR 540

Query: 541 LSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575
           LSNFYW+MC LSG NFLHYLFWALKYKYRSTQGNK
Sbjct: 541 LSNFYWLMCALSGFNFLHYLFWALKYKYRSTQGNK 573

BLAST of Chy3G059610 vs. NCBI nr
Match: XP_022946066.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata])

HSP 1 Score: 853 bits (2203), Expect = 2.09e-307
Identity = 444/577 (76.95%), Postives = 500/577 (86.66%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           ME VKEE DG +WEGYVDWR+RPAV+GRHGGM AAGFVLGVE LENLAFLANASNLVM+L
Sbjct: 1   MEVVKEEGDGGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYM FSP +SANHVT FMGTAFLLALLGGF+SDAFFTTYYVFLFSS +EFLGLV+LTV+
Sbjct: 61  RKYMQFSPTESANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AK +++                 AV+F+GLYLVA+GVGGIKGSLP HGAEQF+E T +GR
Sbjct: 121 AKAATAASA--------------AVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           + RSSFFNYFVFSLSCGA+VAVT VVW+EDNLGW+WGFGISTI+I +SIPLF AGS FYR
Sbjct: 181 RGRSSFFNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIP G+PLTTILKVLVAATLN RS  K A+NAVAS+ RSPSTT TPK        E   
Sbjct: 241 NKIPAGAPLTTILKVLVAATLNGRSRNKTASNAVASMTRSPSTT-TPKPTK-----EPTH 300

Query: 301 TTP--TQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLST 360
           TTP  TQ+LKFLNKA+Q PPFHPSL CTTQQLEDVK+V+K+LPIFACTIILNSCLAQLST
Sbjct: 301 TTPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLST 360

Query: 361 FSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQ 420
           FS+EQA+TMNTKI S+K+PPASLP+FP+LFII+LAPLY+HLIIPFAR+LTRTE+GITHLQ
Sbjct: 361 FSVEQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQ 420

Query: 421 RIGVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLA 480
           RIGVGLLLS++AMA+AAL+E KRKA+ADA+P  H PLPITFLWI FQYLFLGSADLFTLA
Sbjct: 421 RIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLA 480

Query: 481 GLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINH 540
           GLLEFFFTEAP+TMRSLATALSWASLAVGYY SSVIVS+VN  +AK+ H PWLS  +INH
Sbjct: 481 GLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINH 540

Query: 541 YRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575
           Y L  FYW+MC LS LNFLH+LFWA+KYKYRST  +K
Sbjct: 541 YDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Chy3G059610 vs. NCBI nr
Match: XP_023546035.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852 bits (2202), Expect = 2.96e-307
Identity = 447/577 (77.47%), Postives = 498/577 (86.31%), Query Frame = 0

Query: 1   MEAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 60
           ME VKEE D  +WEGYVDWR+RPAV+GRHGGM AAGFVLGVE LENLAFLANASNLVM+L
Sbjct: 1   MEVVKEEGDPGVWEGYVDWRRRPAVRGRHGGMRAAGFVLGVEVLENLAFLANASNLVMFL 60

Query: 61  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 120
           RKYM FSP KSANHVT FMGTAFLLALLGGF+SDAFFTTYYVFLFSS +EFLGLV+LTV+
Sbjct: 61  RKYMQFSPTKSANHVTNFMGTAFLLALLGGFLSDAFFTTYYVFLFSSLLEFLGLVILTVE 120

Query: 121 AKVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGR 180
           AK +++                 AV+F+GLYLVA+GVGGIKGSLPAHGAEQ +E T +GR
Sbjct: 121 AKAATAASA--------------AVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGR 180

Query: 181 KRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYR 240
           K RSSFFNYFVFSLSCGALVAVT VVW+EDNLGW+WGFGISTI+I +SIPLF AGS FYR
Sbjct: 181 KGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYR 240

Query: 241 NKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNA 300
           NKIP  +PLTTILKVLVAA LN R   K A+NAVAS+ RSPSTT TPK        E   
Sbjct: 241 NKIPAAAPLTTILKVLVAAPLNGRRRNKTASNAVASMTRSPSTT-TPKPTK-----EPTH 300

Query: 301 TTP--TQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLST 360
           TTP  TQ+LKFLNKA+Q PPFHPSL CTTQQLEDVKIV+K+LPIFACTIILNSCLAQLST
Sbjct: 301 TTPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLST 360

Query: 361 FSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQ 420
           FS+EQA+TMNTKI S+K+PPASLP+FPVLFII+LAPLY+HLIIPFAR+LTRTE+GITHLQ
Sbjct: 361 FSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQ 420

Query: 421 RIGVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQYLFLGSADLFTLA 480
           RIGVGLLLS++AMA+AAL+E KRKA+ADA+P  H PLPITFLWI FQYLFLGSADLFTLA
Sbjct: 421 RIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLA 480

Query: 481 GLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINH 540
           GLLEFFFTEAP+TMRSLATALSWASLAVGYY SSVIVS+VNH +AK+ H PWLSG +INH
Sbjct: 481 GLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINH 540

Query: 541 YRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGNK 575
           Y L  FYW+MC LS LNFLH+LFWA+KYKYRST  +K
Sbjct: 541 YDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Chy3G059610 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 740.3 bits (1910), Expect = 1.1e-213
Identity = 395/582 (67.87%), Postives = 474/582 (81.44%), Query Frame = 0

Query: 7   ESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLRKYMGF 66
           E + S WEGY DWR R AVKGRHGGM+AA FVL VE LENLA+LANASNLV+YLR+YM  
Sbjct: 4   EEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHM 63

Query: 67  SPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQAKVSS- 126
           SP+KSAN VT FMGTAFLLALLGGF+SDAFF+T+ +FL S+ IEFLGL++LT+QA+  S 
Sbjct: 64  SPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSL 123

Query: 127 ---SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKR 186
              SC+   S  C+EV G + A+LF+GLYLVALGVGGIKGSL +HGAEQFDESTP+GRK+
Sbjct: 124 MPPSCD---SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQ 183

Query: 187 RSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNK 246
           RS+FFNYFVF L+CGALVAVTFVVW+EDN GW+WGFG+STI+IF+SI +F +GS FYRNK
Sbjct: 184 RSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNK 243

Query: 247 IPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPS---TTSTPKQLINKDLIE-- 306
           IP GSPLTTILKVL+AA++   S+   ++NAVAS++ SPS    +   K++ ++  +E  
Sbjct: 244 IPCGSPLTTILKVLLAASVKCCSSGS-SSNAVASMSVSPSNHCVSKGKKEVESQGELEKP 303

Query: 307 --NNATTP----TQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNS 366
               A  P    T SLK LN A    P H  L+CT QQ+EDVKIV+K+LPIFACTI+LN 
Sbjct: 304 RQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNC 363

Query: 367 CLAQLSTFSIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTE 426
           CLAQLSTFS++QAA+MNTKI SLKIPPASLP+FPV+FI+ILAP+YDHLIIPFAR  T+TE
Sbjct: 364 CLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTE 423

Query: 427 AGITHLQRIGVGLLLSVLAMAVAALVETKRKAMADANP--DVHGPLPITFLWIAFQYLFL 486
            G+THLQRIGVGL+LS+LAMAVAALVE KRK +A  +   D    LP+TFLWIA QYLFL
Sbjct: 424 TGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFL 483

Query: 487 GSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRP 546
           GSADLFTLAGLLE+FFTEAP++MRSLAT+LSWASLA+GYY SSVIVS+VN  +  + + P
Sbjct: 484 GSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTP 543

Query: 547 WLSGHNINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRST 572
           WL G +IN Y+L  FYW+MCVLS  NFLHYLFWA++YKYRST
Sbjct: 544 WLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRST 581

BLAST of Chy3G059610 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 696.4 bits (1796), Expect = 1.9e-200
Identity = 368/574 (64.11%), Postives = 447/574 (77.87%), Query Frame = 0

Query: 4   VKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLRKY 63
           V+   D S WEGY DWR + A++GRHGGM+AA FVL VE LENLAFLANASNLV+YL+ +
Sbjct: 3   VEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNF 62

Query: 64  MGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQAKV 123
           M  S A+S++ VTTFM TAFLLALLGGF++DAFF+T+ +FL S+ IEFLGL++LT+QA+ 
Sbjct: 63  MHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARR 122

Query: 124 SS----SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQG 183
            S     C+  ++  C+ VGG + A LF+GLYLV+LG+GGIKGSLP+HGAEQFDE TP+G
Sbjct: 123 PSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKG 182

Query: 184 RKRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFY 243
           RK+RS+FFNY+VF LSCGALVAVTFVVWIEDN GW+WGFG+STISIFLSI +F  GS FY
Sbjct: 183 RKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFY 242

Query: 244 RNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENN 303
           +NKIP GSPLTTI KVL+AA++   S+K  + +  +   +S     TP Q +        
Sbjct: 243 KNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL-------- 302

Query: 304 ATTPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTF 363
               T SL  LNKA++    H  L+CT QQ+EDVKIV+K+LPIF CTI+LN CLAQLST+
Sbjct: 303 ----TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTY 362

Query: 364 SIEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQR 423
           S+ QAATMN KI +  +P ASLPVFPV+F++ILAP YDHLIIPFAR +T++E GITHLQR
Sbjct: 363 SVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQR 422

Query: 424 IGVGLLLSVLAMAVAALVETKRKAMADANP--DVHGPLPITFLWIAFQYLFLGSADLFTL 483
           IGVGL+LS++AMAVAALVE KRK +A      D    LPITFLWIA QYLFLGSADLFTL
Sbjct: 423 IGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTL 482

Query: 484 AGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNIN 543
           AGLLEFFFTEAP++MRSLAT+LSWASLA+GYY SSV+V +VN  +  A   PWL G  +N
Sbjct: 483 AGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLN 542

Query: 544 HYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRST 572
             RL  FYW+MCVLS +NFLHYLFWA +YKY ST
Sbjct: 543 RNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Chy3G059610 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 695.3 bits (1793), Expect = 4.2e-200
Identity = 367/569 (64.50%), Postives = 445/569 (78.21%), Query Frame = 0

Query: 9   DGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLRKYMGFSP 68
           D S WEGY DWR + A++GRHGGM+AA FVL VE LENLAFLANASNLV+YL+ +M  S 
Sbjct: 4   DVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSL 63

Query: 69  AKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQAKVSS--- 128
           A+S++ VTTFM TAFLLALLGGF++DAFF+T+ +FL S+ IEFLGL++LT+QA+  S   
Sbjct: 64  ARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP 123

Query: 129 -SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKRRS 188
             C+  ++  C+ VGG + A LF+GLYLV+LG+GGIKGSLP+HGAEQFDE TP+GRK+RS
Sbjct: 124 PPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRS 183

Query: 189 SFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNKIP 248
           +FFNY+VF LSCGALVAVTFVVWIEDN GW+WGFG+STISIFLSI +F  GS FY+NKIP
Sbjct: 184 TFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIP 243

Query: 249 TGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNATTPT 308
            GSPLTTI KVL+AA++   S+K  + +  +   +S     TP Q +            T
Sbjct: 244 RGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL------------T 303

Query: 309 QSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFSIEQA 368
            SL  LNKA++    H  L+CT QQ+EDVKIV+K+LPIF CTI+LN CLAQLST+S+ QA
Sbjct: 304 NSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQA 363

Query: 369 ATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRIGVGL 428
           ATMN KI +  +P ASLPVFPV+F++ILAP YDHLIIPFAR +T++E GITHLQRIGVGL
Sbjct: 364 ATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGL 423

Query: 429 LLSVLAMAVAALVETKRKAMADANP--DVHGPLPITFLWIAFQYLFLGSADLFTLAGLLE 488
           +LS++AMAVAALVE KRK +A      D    LPITFLWIA QYLFLGSADLFTLAGLLE
Sbjct: 424 VLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLE 483

Query: 489 FFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSGHNINHYRLS 548
           FFFTEAP++MRSLAT+LSWASLA+GYY SSV+V +VN  +  A   PWL G  +N  RL 
Sbjct: 484 FFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GEKLNRNRLD 543

Query: 549 NFYWVMCVLSGLNFLHYLFWALKYKYRST 572
            FYW+MCVLS +NFLHYLFWA +YKY ST
Sbjct: 544 LFYWLMCVLSVVNFLHYLFWAKRYKYIST 559

BLAST of Chy3G059610 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 661.4 bits (1705), Expect = 6.7e-190
Identity = 352/580 (60.69%), Postives = 434/580 (74.83%), Query Frame = 0

Query: 2   EAVKEESDGSIWEGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYLR 61
           E + E +  S W GYVDWR RPA++GRHGGM+AA FVL VE LENLAFLANASNLV+YL 
Sbjct: 30  EQMDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS 89

Query: 62  KYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQA 121
             MGFSP+ +AN VT FMGTAF LALLGGF++DAFFTT++++L S+ IEFLGL++LTVQA
Sbjct: 90  TKMGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQA 149

Query: 122 KVSSSCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRK 181
                  E S++    V       LF+GLYLVALGVGGIKGSLP HGAEQFDE T  GR+
Sbjct: 150 ------HEHSTEPWSRV------FLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRR 209

Query: 182 RRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRN 241
           +RS FFNYF+FSLSCGAL+AVT VVW+EDN GW +GFG+ST +I +S+P+F AGS  YR 
Sbjct: 210 QRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRL 269

Query: 242 KIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLIENNAT 301
           K+P+GSP+TT+ KVL AA   +   ++ +   V    R+    S  KQ  + D       
Sbjct: 270 KVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVTKQNCDGD------- 329

Query: 302 TPTQSLKFLNKAVQNPPFHP-SLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFS 361
                  FL + V+     P  L+CT +Q++DVKIVIK+LPIF  TI+LN CLAQLSTFS
Sbjct: 330 -DGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFS 389

Query: 362 IEQAATMNTKIYSLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRI 421
           ++QA+TMNTK+ S  +PPA+LPVFPV+F++ILAP Y+HL++P AR  T+TE GITHLQRI
Sbjct: 390 VQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRI 449

Query: 422 GVGLLLSVLAMAVAALVETKRKAMA-------DANPDVHGPLPITFLWIAFQYLFLGSAD 481
           G GL+LS++AMAVAALVETKRK +        +++     PLPITFLW+A QY+FLGSAD
Sbjct: 450 GTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSAD 509

Query: 482 LFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNHASAKAWHRPWLSG 541
           LFTLAG++EFFFTEAP+TMRSLAT+LSWASLA+GYYFSSV+VS VN  +    H PWL G
Sbjct: 510 LFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLG 569

Query: 542 HNINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQG 574
            N+N Y L  FYW+MCVLSG+NFLHYLFWA +Y YRS QG
Sbjct: 570 ENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQG 589

BLAST of Chy3G059610 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 8.7e-105
Identity = 231/590 (39.15%), Postives = 348/590 (58.98%), Query Frame = 0

Query: 5   KEESDGSIW----EGYVDWRKRPAVKGRHGGMIAAGFVLGVEALENLAFLANASNLVMYL 64
           +E S+ + W    E  VDWR RP+   +HGGM AA FVLG++A E +   A  +NL+ Y+
Sbjct: 14  EEVSNENNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYV 73

Query: 65  RKYMGFSPAKSANHVTTFMGTAFLLALLGGFISDAFFTTYYVFLFSSFIEFLGLVMLTVQ 124
              M F  +K+AN VT F+GT F+ ALLGG++SDAF  +++  +   F+E  G ++L+VQ
Sbjct: 74  INEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQ 133

Query: 125 AKVSS----SCEEGSSKGCKEVGGKEMAVLFIGLYLVALGVGGIKGSLPAHGAEQFDEST 184
           A +       C     + C+E  G +  + F+ LYLVALG G +K ++ AHGA+QF +S 
Sbjct: 134 AHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSH 193

Query: 185 PQGRKRRSSFFNYFVFSLSCGALVAVTFVVWIEDNLGWQWGFGISTISIFLSIPLFFAGS 244
           P+  KR SS+FN   F+ S G L+A+T +VW++ + G   GFG+S  ++ + I    +G+
Sbjct: 194 PKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGT 253

Query: 245 PFYRNKIPTGSPLTTILKVLVAATLNRRSNKKPATNAVASLARSPSTTSTPKQL-----I 304
            ++RNK P  S  T I  V+VAA L R+                 ++ S P+ L     +
Sbjct: 254 MYFRNKRPRRSIFTPIAHVIVAAILKRKL----------------ASPSDPRMLHGDHHV 313

Query: 305 NKDLIENNATTPTQSLKFLNKAV-----QNPPFHPSLKCTTQQLEDVKIVIKVLPIFACT 364
             D++ ++    T   +FL+KA       N    P   CT  Q+E VK +I ++PIFA T
Sbjct: 314 ANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFAST 373

Query: 365 IILNSCLAQLSTFSIEQAATMNTKI-YSLKIPPASLPVFPVLFIIILAPLYDHLIIPFAR 424
           I+ N+ LAQL TFS++Q ++MNT++  S  IPPASL   P + +I L PLYD  ++PFAR
Sbjct: 374 IVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFAR 433

Query: 425 SLTRTEAGITHLQRIGVGLLLSVLAMAVAALVETKRKAMADANPDVHGPLPITFLWIAFQ 484
            LT   +GI  L RIG+GL LS  +M  AA++E KR+  +  +  +     ++  WI  Q
Sbjct: 434 KLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-----LSIFWITPQ 493

Query: 485 YLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYFSSVIVSVVNH-ASAK 544
           +L  G +++FT  GL+EFF+ ++   M S   AL++ S + G+YFSSV+VSVVN   S  
Sbjct: 494 FLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTS 553

Query: 545 AWHRPWLSGHNINHYRLSNFYWVMCVLSGLNFLHYLFWALKYKYRSTQGN 575
              + WL  +++N  RL  FYW++ VLS LNFL YLFW+ ++  +S++ N
Sbjct: 554 VDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS-RWNIKSSRRN 581

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1571.6e-21267.87Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE42.7e-19964.11Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9FM209.5e-18960.69Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Q93VV51.2e-10339.15Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q9LSF04.8e-10040.54Protein NRT1/ PTR FAMILY 4.1 OS=Arabidopsis thaliana OX=3702 GN=NPF4.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1P8SN460.0e+0097.91NRT1.2-like nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.2C PE=2 SV=1[more]
A0A5D3BLL08.6e-31096.00Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C3A18.6e-31096.00protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 S... [more]
A0A0A0KHD25.7e-25398.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G404120 PE=3 SV=1[more]
A0A6J1G2M64.3e-24576.70protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 ... [more]
Match NameE-valueIdentityDescription
XP_011657481.10.097.91protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >APY23675.1 NRT1.2-like nitrate t... [more]
XP_008456458.10.096.00PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protei... [more]
XP_038886228.10.091.48protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida][more]
XP_022946066.12.09e-30776.95protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata][more]
XP_023546035.12.96e-30777.47protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G69850.11.1e-21367.87nitrate transporter 1:2 [more]
AT1G27040.11.9e-20064.11Major facilitator superfamily protein [more]
AT1G27040.24.2e-20064.50Major facilitator superfamily protein [more]
AT5G62730.16.7e-19060.69Major facilitator superfamily protein [more]
AT1G59740.18.7e-10539.15Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 102..523
e-value: 5.6E-102
score: 341.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 15..572
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 3..574
e-value: 9.3E-168
score: 560.7
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 49..569
NoneNo IPR availablePANTHERPTHR11654:SF490PROTEIN NRT1/ PTR FAMILY 4.6-LIKEcoord: 15..572
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 32..563
e-value: 0.0
score: 631.366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G059610.1Chy3G059610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity