Chy3G058080 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G058080
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	DNA-directed RNA polymerase I subunit RPA1-like
Location	chrH03: 11466602 .. 11467127 (-)
RNA-Seq Expression	Chy3G058080
Synteny	Chy3G058080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTGGATTAAAATGGCACAAATGCCTAGAGTTAAATCTAAGAGAAAGAAAAGAGAATAGTATGAAAAATGCCCTTTTCCGTCATGACGACTTTATAACAAGAAGAGATATAGAAAGAAATTTCAAAGCATTATGCAATGAGGAAGACTCATTGAAAGAAAAATTAGTTAACCTCTATATCTTAGAAACCTTTTTAATTCCTAAGCAATAACACAACCACATAAATCTCCAACATCTAGACATATTGGATCATGAAGAAACATTCAAAGACTATCCATGGGGGAGATCACCATACATCCTAAAATGTCAATTCTTCAAGAAAGCATCATACAATGACAAGGATGCTATATACTTTCAAGGATTTCGCCTAGCTTTAATTTATTGGATTTTTGAGACAATACCAAAACTTTTCAATTTAGATGTTGGGTTTGGAAGAACGCTTGGAATTCAAGGGCCAACAATAATGACATGAGAATGTTTGGAGTCAAGTGACTGGAGGACTCTAAACATGAACATTTTATTAA

mRNA sequence

ATGACTGGATTAAAATGGCACAAATGCCTAGAGTTAAATCTAAGAGAAAGAAAAGAGAATAGTATGAAAAATGCCCTTTTCCGTCATGACGACTTTATAACAAGAAGAGATATAGAAAGAAATTTCAAAGCATTATGCAATGAGGAAGACTCATTGAAAGAAAAATTAGTTAACCTCTATATCTTAGAAACCTTTTTAATTCCTAAGCAATAACACAACCACATAAATCTCCAACATCTAGACATATTGGATCATGAAGAAACATTCAAAGACTATCCATGGGGGAGATCACCATACATCCTAAAATGTCAATTCTTCAAGAAAGCATCATACAATGACAAGGATGCTATATACTTTCAAGGATTTCGCCTAGCTTTAATTTATTGGATTTTTGAGACAATACCAAAACTTTTCAATTTAGATGTTGGGTTTGGAAGAACGCTTGGAATTCAAGGGCCAACAATAATGACATGAGAATGTTTGGAGTCAAGTGACTGGAGGACTCTAAACATGAACATTTTATTAA

Coding sequence (CDS)

ATGACTGGATTAAAATGGCACAAATGCCTAGAGTTAAATCTAAGAGAAAGAAAAGAGAATAGTATGAAAAATGCCCTTTTCCGTCATGACGACTTTATAACAAGAAGAGATATAGAAAGAAATTTCAAAGCATTATGCAATGAGGAAGACTCATTGAAAGAAAAATTAGTTAACCTCTATATCTTAGAAACCTTTTTAATTCCTAAGCAATAACACAACCACATAAATCTCCAACATCTAGACATATTGGATCATGAAGAAACATTCAAAGACTATCCATGGGGGAGATCACCATACATCCTAAAATGTCAATTCTTCAAGAAAGCATCATACAATGACAAGGATGCTATATACTTTCAAGGATTTCGCCTAGCTTTAATTTATTGGATTTTTGAGACAATACCAAAACTTTTCAATTTAGATGTTGGGTTTGGAAGAACGCTTGGAATTCAAGGGCCAACAATAATGACATGAGAATGTTTGGAGTCAAGTGACTGGAGGACTCTAAACATGAACATTTTATTAA

Protein sequence

MTGLKWHKCLELNLRERKENSMKNALFRHDDFITRRDIERNFKALCNEEDSLKEKLVNLYILETFLIPKQ*HNHINLQHLDILDHEETFKDYPWGRSPYILKCQFFKKASYNDKDAIYFQGFRLALIYWIFETIPKLFNLDVGFGRTLGIQGPTIMT*ECLESSDWRTLNMNILLX

Homology

[Close]

BLAST of Chy3G058080 vs. ExPASy TrEMBL
Match: A0A5A7T9I0 (DNA-directed RNA polymerase I subunit RPA1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G001480 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.5e-31
Identity = 65/94 (69.15%), Postives = 74/94 (78.72%), Query Frame = 0


Query: 72  HNHINLQHLDILDHEETFKDYPWGRSPYILKCQFFKKASYNDKDAIYFQGFRLALIYWIF 131
           HNHINL HLDIL++EE FK YPWGR  YIL  QF KKASYNDKD +Y QGF LAL+YW  
Sbjct: 235 HNHINLLHLDILNNEEIFKGYPWGRLSYILTYQFLKKASYNDKDVVYLQGFPLALVYWAL 294

Query: 132 ETIPKLFNLDVGFGRTLGIQGPTIMT*ECLESSD 166
           E IP+  NLDVGFGR L I+GP I+T ECL++SD
Sbjct: 295 EKIPRFSNLDVGFGRKLAIEGPPIVTWECLKTSD 328

[Close]

BLAST of Chy3G058080 vs. ExPASy TrEMBL
Match: A0A5D3CNI7 (TF-B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005170 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.5e-10
Identity = 48/152 (31.58%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 1   MTGLKWHKCLELNLRERKENSMKNALFRHDDFITRRDIERNFKALCNEEDSLKEKLVNLY 60
           +TGL   +   +++ + ++       F  +  I R  +   F  +    +    K+  LY
Sbjct: 723 ITGLNCGELPAIDMSKIQKGKFNKRYFGGEKTIRRTKLHEVFTEMDKGRNKDVVKMAKLY 782

Query: 61  ILETFLIPKQ*HNHINLQHLDILDHEETFKDYPWGRSPYILKCQFFKKA-SYNDKDAIYF 120
           ILE F++ KQ    IN ++  ++D +E F  YPWGR  Y +   F KKA   ND  AI  
Sbjct: 783 ILEMFILGKQLRTGINHEYTLLIDDKEQFDSYPWGRISYEITIDFVKKAIKSNDASAIGV 842

Query: 121 QGFRLALIYWIFETIPKL------FNLDVGFG 146
            GF  AL  W +ETIP L      F + + FG
Sbjct: 843 GGFPFALCVWAYETIPLLALNSNFFAMRISFG 874

[Close]

BLAST of Chy3G058080 vs. ExPASy TrEMBL
Match: A0A1S3B0L9 (uncharacterized protein LOC103484737 isoform X5 OS=Cucumis melo OX=3656 GN=LOC103484737 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.5e-10
Identity = 48/152 (31.58%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 1   MTGLKWHKCLELNLRERKENSMKNALFRHDDFITRRDIERNFKALCNEEDSLKEKLVNLY 60
           +TGL   +   +++ + ++       F  +  I R  +   F  +    +    K+  LY
Sbjct: 631 ITGLNCGELPAIDMSKIQKGKFNKRYFGGEKTIRRTKLHEVFTEMDKGRNKDVVKMAKLY 690

Query: 61  ILETFLIPKQ*HNHINLQHLDILDHEETFKDYPWGRSPYILKCQFFKKA-SYNDKDAIYF 120
           ILE F++ KQ    IN ++  ++D +E F  YPWGR  Y +   F KKA   ND  AI  
Sbjct: 691 ILEMFILGKQLRTGINHEYTLLIDDKEQFDSYPWGRISYEITIDFVKKAIKSNDASAIGV 750

Query: 121 QGFRLALIYWIFETIPKL------FNLDVGFG 146
            GF  AL  W +ETIP L      F + + FG
Sbjct: 751 GGFPFALCVWAYETIPLLALNSNFFAMRISFG 782

[Close]

BLAST of Chy3G058080 vs. ExPASy TrEMBL
Match: A0A1S3B181 (uncharacterized protein LOC103484737 isoform X7 OS=Cucumis melo OX=3656 GN=LOC103484737 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.5e-10
Identity = 48/152 (31.58%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 1   MTGLKWHKCLELNLRERKENSMKNALFRHDDFITRRDIERNFKALCNEEDSLKEKLVNLY 60
           +TGL   +   +++ + ++       F  +  I R  +   F  +    +    K+  LY
Sbjct: 629 ITGLNCGELPAIDMSKIQKGKFNKRYFGGEKTIRRTKLHEVFTEMDKGRNKDVVKMAKLY 688

Query: 61  ILETFLIPKQ*HNHINLQHLDILDHEETFKDYPWGRSPYILKCQFFKKA-SYNDKDAIYF 120
           ILE F++ KQ    IN ++  ++D +E F  YPWGR  Y +   F KKA   ND  AI  
Sbjct: 689 ILEMFILGKQLRTGINHEYTLLIDDKEQFDSYPWGRISYEITIDFVKKAIKSNDASAIGV 748

Query: 121 QGFRLALIYWIFETIPKL------FNLDVGFG 146
            GF  AL  W +ETIP L      F + + FG
Sbjct: 749 GGFPFALCVWAYETIPLLALNSNFFAMRISFG 780

[Close]

BLAST of Chy3G058080 vs. ExPASy TrEMBL
Match: A0A1S3B065 (uncharacterized protein LOC103484737 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103484737 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.5e-10
Identity = 48/152 (31.58%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 1   MTGLKWHKCLELNLRERKENSMKNALFRHDDFITRRDIERNFKALCNEEDSLKEKLVNLY 60
           +TGL   +   +++ + ++       F  +  I R  +   F  +    +    K+  LY
Sbjct: 647 ITGLNCGELPAIDMSKIQKGKFNKRYFGGEKTIRRTKLHEVFTEMDKGRNKDVVKMAKLY 706

Query: 61  ILETFLIPKQ*HNHINLQHLDILDHEETFKDYPWGRSPYILKCQFFKKA-SYNDKDAIYF 120
           ILE F++ KQ    IN ++  ++D +E F  YPWGR  Y +   F KKA   ND  AI  
Sbjct: 707 ILEMFILGKQLRTGINHEYTLLIDDKEQFDSYPWGRISYEITIDFVKKAIKSNDASAIGV 766

Query: 121 QGFRLALIYWIFETIPKL------FNLDVGFG 146
            GF  AL  W +ETIP L      F + + FG
Sbjct: 767 GGFPFALCVWAYETIPLLALNSNFFAMRISFG 798

[Close]

BLAST of Chy3G058080 vs. NCBI nr
Match: KAA0038287.1 (DNA-directed RNA polymerase I subunit RPA1-like [Cucumis melo var. makuwa])

HSP 1 Score: 145 bits (366), Expect = 6.65e-39
Identity = 65/94 (69.15%), Postives = 74/94 (78.72%), Query Frame = 0


Query: 72  HNHINLQHLDILDHEETFKDYPWGRSPYILKCQFFKKASYNDKDAIYFQGFRLALIYWIF 131
           HNHINL HLDIL++EE FK YPWGR  YIL  QF KKASYNDKD +Y QGF LAL+YW  
Sbjct: 235 HNHINLLHLDILNNEEIFKGYPWGRLSYILTYQFLKKASYNDKDVVYLQGFPLALVYWAL 294

Query: 132 ETIPKLFNLDVGFGRTLGIQGPTIMT*ECLESSD 165
           E IP+  NLDVGFGR L I+GP I+T ECL++SD
Sbjct: 295 EKIPRFSNLDVGFGRKLAIEGPPIVTWECLKTSD 328

[Close]

BLAST of Chy3G058080 vs. NCBI nr
Match: XP_038891747.1 (pescadillo homolog [Benincasa hispida])

HSP 1 Score: 97.8 bits (242), Expect = 7.20e-21
Identity = 42/92 (45.65%), Postives = 56/92 (60.87%), Query Frame = 0


Query: 82  ILDHEETFKDYPWGRSPYILKCQFFKKASYNDKDAIYFQGFRLALIYWIFETIPKLFNLD 141
           +LD EE F+ YPWGR  + L  +FFKK   N   +I+ QGF L L+YW FE IP+L N  
Sbjct: 1   MLDDEERFRSYPWGRVSFELTIEFFKKVVINKPSSIFLQGFPLGLVYWAFEIIPRLSNPT 60

Query: 142 VGFGRTLGIQGPTIMT*ECLESSDWRTLNMNI 173
           +GF R +   GP +   E  E +DW+ +N NI
Sbjct: 61  IGFARRIKSDGPRLTQWESQEPNDWQHINNNI 92

[Close]

BLAST of Chy3G058080 vs. NCBI nr
Match: XP_024031030.1 (uncharacterized protein LOC21394043 [Morus notabilis] >XP_024031031.1 uncharacterized protein LOC21394043 [Morus notabilis] >XP_024031032.1 uncharacterized protein LOC21394043 [Morus notabilis] >XP_024031033.1 uncharacterized protein LOC21394043 [Morus notabilis])

HSP 1 Score: 83.2 bits (204), Expect = 6.81e-15
Identity = 48/152 (31.58%), Postives = 77/152 (50.66%), Query Frame = 0


Query: 19  ENSMKNALFRHDDFITRRDIERNFKALCNEEDSLKEKLVNLYILETFLIPKQ*HNHINLQ 78
           E++ K   FR    + R  +   F+A     D    KL  LY LE+ LIPK+  N+I+  
Sbjct: 197 ESTTKRKFFRKGKSVQRIKLNDVFRANRGGTDEDIVKLAKLYCLESLLIPKKIENNIDPN 256

Query: 79  HLDILDHEETFKDYPWGRSPYILKCQFFKKASYNDKDAIY-FQGFRLALIYWIFETIPKL 138
           HL ++D+ E F +YPWGR  Y +   + K++  + +   Y   GF  A+I W +ETIP L
Sbjct: 257 HLKMVDNPELFDNYPWGRLSYEMTIAYIKRSIKSQEAEAYGIGGFPYAVIVWAYETIPTL 316

Query: 139 FNLDVGFGRTLGIQGPTIMT*ECLESSDWRTL 169
              ++   + +G   P I+  E  +   +R +
Sbjct: 317 IKKNIA--KRIGNGIPRIINWEADQQPSFREI 346

[Close]

BLAST of Chy3G058080 vs. NCBI nr
Match: EXC30509.1 (hypothetical protein L484_010758 [Morus notabilis])

HSP 1 Score: 83.2 bits (204), Expect = 6.83e-15
Identity = 48/152 (31.58%), Postives = 77/152 (50.66%), Query Frame = 0


Query: 19  ENSMKNALFRHDDFITRRDIERNFKALCNEEDSLKEKLVNLYILETFLIPKQ*HNHINLQ 78
           E++ K   FR    + R  +   F+A     D    KL  LY LE+ LIPK+  N+I+  
Sbjct: 197 ESTTKRKFFRKGKSVQRIKLNDVFRANRGGTDEDIVKLAKLYCLESLLIPKKIENNIDPN 256

Query: 79  HLDILDHEETFKDYPWGRSPYILKCQFFKKASYNDKDAIY-FQGFRLALIYWIFETIPKL 138
           HL ++D+ E F +YPWGR  Y +   + K++  + +   Y   GF  A+I W +ETIP L
Sbjct: 257 HLKMVDNPELFDNYPWGRLSYEMTIAYIKRSIKSQEAEAYGIGGFPYAVIVWAYETIPTL 316

Query: 139 FNLDVGFGRTLGIQGPTIMT*ECLESSDWRTL 169
              ++   + +G   P I+  E  +   +R +
Sbjct: 317 IKKNIA--KRIGNGIPRIINWEADQQPSFREI 346

[Close]

BLAST of Chy3G058080 vs. NCBI nr
Match: XP_022132727.1 (uncharacterized protein LOC111005524 [Momordica charantia] >XP_022132728.1 uncharacterized protein LOC111005524 [Momordica charantia] >XP_022132729.1 uncharacterized protein LOC111005524 [Momordica charantia] >XP_022132730.1 uncharacterized protein LOC111005524 [Momordica charantia])

HSP 1 Score: 79.7 bits (195), Expect = 1.06e-13
Identity = 49/142 (34.51%), Postives = 66/142 (46.48%), Query Frame = 0


Query: 33  ITRRDIERNFKALCNEEDSLKEKLVNLYILETFLIPKQ*HNHINLQHLDILDHEETFKDY 92
           I R  +   F  +    D    K+  LYILE FL+ KQ    IN ++  ++D +E F+ Y
Sbjct: 369 IKRTKLPEIFMEMDKGRDKDVVKMAKLYILEMFLLGKQITTGINHEYTLLIDDDEQFEGY 428

Query: 93  PWGRSPYILKCQFFKKA-SYNDKDAIYFQGFRLALIYWIFETIPKLFNLDVGFGRTLGIQ 152
           PWGR  Y +   F KKA   ND  AI   GF  AL+ W +ETIP L      F   +   
Sbjct: 429 PWGRVSYEITIDFVKKAIKSNDASAIGIGGFPYALLVWAYETIPLLSLNSNLFATKISSG 488

Query: 153 GPTIMT*ECLESSDWRTLNMNI 173
            P +         +WR L+  I
Sbjct: 489 MPRMNNWVANVHPEWRDLSEKI 510

[Close]

BLAST of Chy3G058080 vs. TAIR 10
Match: AT3G32960.1 (Domain of unknown function (DUF1985) )

HSP 1 Score: 43.1 bits (100), Expect = 2.7e-04
Identity = 33/111 (29.73%), Postives = 50/111 (45.05%), Query Frame = 0


Query: 48  EEDSLKEK--LVNLYILETFLIPKQ*HNHINLQHLDILDHEETFKDYPWGRSPYILKCQF 107
           +EDS +E+  L  L ++E+  +         +++L+     E   +YPWG   + +    
Sbjct: 49  DEDSSEERFCLAMLLLIESTFLTLYKGCKFPIENLERAQDVEKLTNYPWGEDAFNVPMSS 108

Query: 108 FKK--ASYNDKDAIYFQGFRLALIYWIFETIPKLFNLDVGFGRTLGIQGPT 155
            KK  AS   K      GF LAL  WI E+IP    L   + R   I+ PT
Sbjct: 109 IKKNVASNMMKPKHDIHGFPLALHIWILESIPM---LQTTYNRISLIERPT 156

[Close]

BLAST of Chy3G058080 vs. TAIR 10
Match: AT5G28810.1 (Domain of unknown function (DUF1985) )

HSP 1 Score: 42.7 bits (99), Expect = 3.5e-04
Identity = 29/88 (32.95%), Postives = 39/88 (44.32%), Query Frame = 0


Query: 89  FKDYPWG--------RSPYILKCQFFKKASYNDKDAIYFQGFRLALIYWIFETIPKLFNL 148
           F+ YPWG        RS  I+KC         D D+    G   AL+ WI+E++P     
Sbjct: 139 FEKYPWGRVAFDSLLRSVKIVKC---------DGDSYVIHGCVQALLVWIYESVP----- 198

Query: 149 DVGFGRTLGIQGPTIMT*ECLESSDWRT 169
             G G   G  G T +T  C+   DWR+
Sbjct: 199 --GIGEACGF-GKTTLT--CVPLLDWRS 207

The following BLAST results are available for this feature:

BLAST of Chy3G058080 vs.


Match Name	E-value	Identity	Description

BLAST of Chy3G058080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T9I0	1.5e-31	69.15	DNA-directed RNA polymerase I subunit RPA1-like OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3CNI7	1.5e-10	31.58	TF-B3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3B0L9	1.5e-10	31.58	uncharacterized protein LOC103484737 isoform X5 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3B181	1.5e-10	31.58	uncharacterized protein LOC103484737 isoform X7 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A1S3B065	1.5e-10	31.58	uncharacterized protein LOC103484737 isoform X4 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Chy3G058080 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0038287.1	6.65e-39	69.15	DNA-directed RNA polymerase I subunit RPA1-like [Cucumis melo var. makuwa]	[more]
XP_038891747.1	7.20e-21	45.65	pescadillo homolog [Benincasa hispida]	[more]
XP_024031030.1	6.81e-15	31.58	uncharacterized protein LOC21394043 [Morus notabilis] >XP_024031031.1 uncharacte...	[more]
EXC30509.1	6.83e-15	31.58	hypothetical protein L484_010758 [Morus notabilis]	[more]
XP_022132727.1	1.06e-13	34.51	uncharacterized protein LOC111005524 [Momordica charantia] >XP_022132728.1 uncha...	[more]

BLAST of Chy3G058080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G32960.1	2.7e-04	29.73	Domain of unknown function (DUF1985)	[more]
AT5G28810.1	3.5e-04	32.95	Domain of unknown function (DUF1985)	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 35..55
IPR015410	Domain of unknown function DUF1985	PFAM	PF09331	DUF1985	coord: 1..96 e-value: 1.2E-11 score: 44.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G058080.1	Chy3G058080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032774	RNA biosynthetic process
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity