Chy3G056520 (gene) Cucumber (hystrix) v1

Overview
NameChy3G056520
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionTP53-regulated inhibitor of apoptosis 1
LocationchrH03: 9295198 .. 9295768 (-)
RNA-Seq ExpressionChy3G056520
SyntenyChy3G056520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGCGAGAACAAACGACGACTTCGTTGGCCGAGAGAATTTCCAAGAGCCGCCGCGTCGTTCAGGCCGCTGTTGCTTCTCGGATGGTCATTTGATGGAGTGATAGTATCTCAAGCAATGGGCATCAAGAGAAAGAGTAAAAGCTCCGCTTCTGCAACATCGCCGTGTGCTGATCTCAGGGCTGCTTATCACAATTGTTTCAATCGGTTCGTTTCCATTTCCATTCATTCCCTAAACCCCTTTTGAACCCTAATTCGTTCTCACCTCGGCGTTGTTCTACTGATCTCCCGTTTCTAATTTGCAGATGGTACTCGGAGAAGTTCGTAAAAGGTAATTGGGATGAAGAACCCTGCGTCTCCGAGTGGCAGAAGTACAGGGCTTGCCTTTATGTATGTCTTTGAACTAACCTTGATTATTACTAGGTCTTGTAAATATCATTATTTGTGCTTTTTTTCGAGATCTGATATATAGTCTAATTGATTGACAGGAACATTTGGATGATAAGAAGTTGAAGCGTTTCTTGGAAGAGGAAACGCTTGTTCATTCCTCCATGAAATCTGATGGGAGTTAA

mRNA sequence

ATGCGCGAGAACAAACGACGACTTCGTTGGCCGAGAGAATTTCCAAGAGCCGCCGCGTCGTTCAGGCCGCTGTTGCTTCTCGGATGGTCATTTGATGGAGTGATAGTATCTCAAGCAATGGGCATCAAGAGAAAGAGTAAAAGCTCCGCTTCTGCAACATCGCCGTGTGCTGATCTCAGGGCTGCTTATCACAATTGTTTCAATCGATGGTACTCGGAGAAGTTCGTAAAAGGTAATTGGGATGAAGAACCCTGCGTCTCCGAGTGGCAGAAGTACAGGGCTTGCCTTTATGAACATTTGGATGATAAGAAGTTGAAGCGTTTCTTGGAAGAGGAAACGCTTGTTCATTCCTCCATGAAATCTGATGGGAGTTAA

Coding sequence (CDS)

ATGCGCGAGAACAAACGACGACTTCGTTGGCCGAGAGAATTTCCAAGAGCCGCCGCGTCGTTCAGGCCGCTGTTGCTTCTCGGATGGTCATTTGATGGAGTGATAGTATCTCAAGCAATGGGCATCAAGAGAAAGAGTAAAAGCTCCGCTTCTGCAACATCGCCGTGTGCTGATCTCAGGGCTGCTTATCACAATTGTTTCAATCGATGGTACTCGGAGAAGTTCGTAAAAGGTAATTGGGATGAAGAACCCTGCGTCTCCGAGTGGCAGAAGTACAGGGCTTGCCTTTATGAACATTTGGATGATAAGAAGTTGAAGCGTTTCTTGGAAGAGGAAACGCTTGTTCATTCCTCCATGAAATCTGATGGGAGTTAA

Protein sequence

MRENKRRLRWPREFPRAAASFRPLLLLGWSFDGVIVSQAMGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS*
Homology
BLAST of Chy3G056520 vs. ExPASy Swiss-Prot
Match: Q9SMZ9 (Uncharacterized protein At4g33100 OS=Arabidopsis thaliana OX=3702 GN=At4g33100 PE=3 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 3.0e-23
Identity = 48/68 (70.59%), Postives = 56/68 (82.35%), Query Frame = 0

Query: 43  KRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEHLDD 102
           K+ S S+ S+TSPCADLR AYHNCFN+WYSEKFVKG WD+E CV+EW+KYR CL E+LD 
Sbjct: 6   KKDSTSARSSTSPCADLRNAYHNCFNKWYSEKFVKGQWDKEECVAEWKKYRDCLSENLDG 65

Query: 103 KKLKRFLE 111
           K L R LE
Sbjct: 66  KLLTRILE 73

BLAST of Chy3G056520 vs. ExPASy Swiss-Prot
Match: O60200 (Mitochondrial distribution and morphology protein 35 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MDM35 PE=1 SV=3)

HSP 1 Score: 59.7 bits (143), Expect = 2.7e-08
Identity = 27/63 (42.86%), Postives = 35/63 (55.56%), Query Frame = 0

Query: 49  SASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRF 108
           SAS    C DL+  Y +CFN WYSEKF+KG   E  C  +W  Y  C+   L  + +K  
Sbjct: 6   SASFAPECTDLKTKYDSCFNEWYSEKFLKGKSVENECSKQWYAYTTCVNAALVKQGIKPA 65

Query: 109 LEE 112
           L+E
Sbjct: 66  LDE 68

BLAST of Chy3G056520 vs. ExPASy Swiss-Prot
Match: Q6DD38 (TP53-regulated inhibitor of apoptosis 1-B OS=Xenopus laevis OX=8355 GN=triap1-b PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 6.1e-08
Identity = 21/55 (38.18%), Postives = 35/55 (63.64%), Query Frame = 0

Query: 51  SATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKL 106
           S    C D++  Y  CFNRW++EKF+KG+   +PC   +++YR C+ + + DK +
Sbjct: 3   SVGEECTDMKREYDQCFNRWFAEKFLKGDGSGDPCTELFRRYRECVQKAIKDKDI 57

BLAST of Chy3G056520 vs. ExPASy Swiss-Prot
Match: A9ULB4 (TP53-regulated inhibitor of apoptosis 1 OS=Xenopus tropicalis OX=8364 GN=triap1 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.0e-07
Identity = 21/55 (38.18%), Postives = 34/55 (61.82%), Query Frame = 0

Query: 51  SATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKL 106
           S    C D++  Y  CFNRW++EKF+KG    +PC   +++YR C+ + + DK +
Sbjct: 3   SVGEECTDMKREYDQCFNRWFAEKFLKGECSGDPCTELFRRYRDCVQKAIKDKDI 57

BLAST of Chy3G056520 vs. ExPASy Swiss-Prot
Match: Q6INR6 (TP53-regulated inhibitor of apoptosis 1-A OS=Xenopus laevis OX=8355 GN=triap1-a PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.8e-07
Identity = 21/55 (38.18%), Postives = 34/55 (61.82%), Query Frame = 0

Query: 51  SATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKL 106
           S    C D++  Y  CFNRW++EKF+KG    +PC   +++YR C+ + + DK +
Sbjct: 3   SVGEECTDMKRDYDQCFNRWFAEKFLKGAGSGDPCTELFRRYRECVQKAIKDKDI 57

BLAST of Chy3G056520 vs. ExPASy TrEMBL
Match: I3XLL9 (Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=ABI2 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.6e-56
Identity = 106/112 (94.64%), Postives = 110/112 (98.21%), Query Frame = 0

Query: 13  EFPRAAASFRPLLLLGWSFDGVIVSQAMGIKRKSKSSASATSPCADLRAAYHNCFNRWYS 72
           + PRAAASFRPL+LLGWSFDG+IVSQAMG KR+SKSSASATSPCADLRAAYHNCFNRWYS
Sbjct: 559 KIPRAAASFRPLMLLGWSFDGMIVSQAMGFKRESKSSASATSPCADLRAAYHNCFNRWYS 618

Query: 73  EKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 125
           EKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS
Sbjct: 619 EKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 670

BLAST of Chy3G056520 vs. ExPASy TrEMBL
Match: A0A0A0KGU3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G363580 PE=3 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.3e-50
Identity = 96/100 (96.00%), Postives = 99/100 (99.00%), Query Frame = 0

Query: 25  LLLGWSFDGVIVSQAMGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEP 84
           +LLGWSFDG+IVSQAMG KR+SKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEP
Sbjct: 1   MLLGWSFDGMIVSQAMGFKRESKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEP 60

Query: 85  CVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 125
           CVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS
Sbjct: 61  CVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 100

BLAST of Chy3G056520 vs. ExPASy TrEMBL
Match: A0A5A7V9A7 (Protein phosphatase 2C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00330 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-42
Identity = 84/85 (98.82%), Postives = 85/85 (100.00%), Query Frame = 0

Query: 40  MGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 99
           MGIKR+SKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH
Sbjct: 1   MGIKRESKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 60

Query: 100 LDDKKLKRFLEEETLVHSSMKSDGS 125
           LDDKKLKRFLEEETLVHSSMKSDGS
Sbjct: 61  LDDKKLKRFLEEETLVHSSMKSDGS 85

BLAST of Chy3G056520 vs. ExPASy TrEMBL
Match: A0A1S3BCF6 (uncharacterized protein At4g33100 OS=Cucumis melo OX=3656 GN=LOC103488506 PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.3e-42
Identity = 84/85 (98.82%), Postives = 85/85 (100.00%), Query Frame = 0

Query: 40  MGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 99
           MGIKR+SKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH
Sbjct: 1   MGIKRESKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 60

Query: 100 LDDKKLKRFLEEETLVHSSMKSDGS 125
           LDDKKLKRFLEEETLVHSSMKSDGS
Sbjct: 61  LDDKKLKRFLEEETLVHSSMKSDGS 85

BLAST of Chy3G056520 vs. ExPASy TrEMBL
Match: A0A6J1BQK5 (uncharacterized protein At4g33100 OS=Momordica charantia OX=3673 GN=LOC111004711 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.8e-37
Identity = 74/85 (87.06%), Postives = 81/85 (95.29%), Query Frame = 0

Query: 40  MGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 99
           MGI R+SKSSASATSPCADLRAAYHNCFNRWYSEKFV+G WDEEPCVSEWQKYRACL EH
Sbjct: 1   MGINRESKSSASATSPCADLRAAYHNCFNRWYSEKFVRGQWDEEPCVSEWQKYRACLSEH 60

Query: 100 LDDKKLKRFLEEETLVHSSMKSDGS 125
           L+DKKLKRFLE+E+LVHSS+K+D S
Sbjct: 61  LEDKKLKRFLEDESLVHSSVKADAS 85

BLAST of Chy3G056520 vs. NCBI nr
Match: AFL56849.1 (protein phosphatase 2C [Cucumis sativus])

HSP 1 Score: 229 bits (584), Expect = 5.48e-69
Identity = 106/112 (94.64%), Postives = 110/112 (98.21%), Query Frame = 0

Query: 13  EFPRAAASFRPLLLLGWSFDGVIVSQAMGIKRKSKSSASATSPCADLRAAYHNCFNRWYS 72
           + PRAAASFRPL+LLGWSFDG+IVSQAMG KR+SKSSASATSPCADLRAAYHNCFNRWYS
Sbjct: 559 KIPRAAASFRPLMLLGWSFDGMIVSQAMGFKRESKSSASATSPCADLRAAYHNCFNRWYS 618

Query: 73  EKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 124
           EKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS
Sbjct: 619 EKFVKGNWDEEPCVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 670

BLAST of Chy3G056520 vs. NCBI nr
Match: XP_004144203.1 (uncharacterized protein At4g33100 [Cucumis sativus] >KGN47602.1 hypothetical protein Csa_019023 [Cucumis sativus])

HSP 1 Score: 209 bits (533), Expect = 6.16e-68
Identity = 96/100 (96.00%), Postives = 99/100 (99.00%), Query Frame = 0

Query: 25  LLLGWSFDGVIVSQAMGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEP 84
           +LLGWSFDG+IVSQAMG KR+SKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEP
Sbjct: 1   MLLGWSFDGMIVSQAMGFKRESKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEP 60

Query: 85  CVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 124
           CVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS
Sbjct: 61  CVSEWQKYRACLYEHLDDKKLKRFLEEETLVHSSMKSDGS 100

BLAST of Chy3G056520 vs. NCBI nr
Match: XP_008445512.1 (PREDICTED: uncharacterized protein At4g33100 [Cucumis melo] >KAA0064723.1 protein phosphatase 2C [Cucumis melo var. makuwa] >TYK00743.1 protein phosphatase 2C [Cucumis melo var. makuwa])

HSP 1 Score: 183 bits (465), Expect = 9.09e-58
Identity = 84/85 (98.82%), Postives = 85/85 (100.00%), Query Frame = 0

Query: 40  MGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 99
           MGIKR+SKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH
Sbjct: 1   MGIKRESKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 60

Query: 100 LDDKKLKRFLEEETLVHSSMKSDGS 124
           LDDKKLKRFLEEETLVHSSMKSDGS
Sbjct: 61  LDDKKLKRFLEEETLVHSSMKSDGS 85

BLAST of Chy3G056520 vs. NCBI nr
Match: XP_038886487.1 (uncharacterized protein At4g33100 [Benincasa hispida])

HSP 1 Score: 166 bits (420), Expect = 6.61e-51
Identity = 77/85 (90.59%), Postives = 81/85 (95.29%), Query Frame = 0

Query: 40  MGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 99
           MGIK++SKSSASATS CADLRAAYHNCFNRWYSEKFVKG+WDEEPC SEWQKYRACL EH
Sbjct: 1   MGIKKESKSSASATSRCADLRAAYHNCFNRWYSEKFVKGHWDEEPCDSEWQKYRACLSEH 60

Query: 100 LDDKKLKRFLEEETLVHSSMKSDGS 124
           L DKKLKRFLEEETLVHSSMK+DGS
Sbjct: 61  LVDKKLKRFLEEETLVHSSMKADGS 85

BLAST of Chy3G056520 vs. NCBI nr
Match: XP_022131556.1 (uncharacterized protein At4g33100 [Momordica charantia] >XP_022131557.1 uncharacterized protein At4g33100 [Momordica charantia] >XP_022131558.1 uncharacterized protein At4g33100 [Momordica charantia] >XP_022131559.1 uncharacterized protein At4g33100 [Momordica charantia])

HSP 1 Score: 165 bits (418), Expect = 1.33e-50
Identity = 74/85 (87.06%), Postives = 81/85 (95.29%), Query Frame = 0

Query: 40  MGIKRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEH 99
           MGI R+SKSSASATSPCADLRAAYHNCFNRWYSEKFV+G WDEEPCVSEWQKYRACL EH
Sbjct: 1   MGINRESKSSASATSPCADLRAAYHNCFNRWYSEKFVRGQWDEEPCVSEWQKYRACLSEH 60

Query: 100 LDDKKLKRFLEEETLVHSSMKSDGS 124
           L+DKKLKRFLE+E+LVHSS+K+D S
Sbjct: 61  LEDKKLKRFLEDESLVHSSVKADAS 85

BLAST of Chy3G056520 vs. TAIR 10
Match: AT4G33100.1 (CONTAINS InterPro DOMAIN/s: Mitochondrial distribution/morphology family 35/apoptosis (InterPro:IPR007918); Has 214 Blast hits to 214 proteins in 102 species: Archae - 0; Bacteria - 0; Metazoa - 110; Fungi - 69; Plants - 29; Viruses - 0; Other Eukaryotes - 6 (source: NCBI BLink). )

HSP 1 Score: 109.4 bits (272), Expect = 2.1e-24
Identity = 48/68 (70.59%), Postives = 56/68 (82.35%), Query Frame = 0

Query: 43  KRKSKSSASATSPCADLRAAYHNCFNRWYSEKFVKGNWDEEPCVSEWQKYRACLYEHLDD 102
           K+ S S+ S+TSPCADLR AYHNCFN+WYSEKFVKG WD+E CV+EW+KYR CL E+LD 
Sbjct: 6   KKDSTSARSSTSPCADLRNAYHNCFNKWYSEKFVKGQWDKEECVAEWKKYRDCLSENLDG 65

Query: 103 KKLKRFLE 111
           K L R LE
Sbjct: 66  KLLTRILE 73

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMZ93.0e-2370.59Uncharacterized protein At4g33100 OS=Arabidopsis thaliana OX=3702 GN=At4g33100 P... [more]
O602002.7e-0842.86Mitochondrial distribution and morphology protein 35 OS=Saccharomyces cerevisiae... [more]
Q6DD386.1e-0838.18TP53-regulated inhibitor of apoptosis 1-B OS=Xenopus laevis OX=8355 GN=triap1-b ... [more]
A9ULB41.0e-0738.18TP53-regulated inhibitor of apoptosis 1 OS=Xenopus tropicalis OX=8364 GN=triap1 ... [more]
Q6INR61.8e-0738.18TP53-regulated inhibitor of apoptosis 1-A OS=Xenopus laevis OX=8355 GN=triap1-a ... [more]
Match NameE-valueIdentityDescription
I3XLL91.6e-5694.64Protein-serine/threonine phosphatase OS=Cucumis sativus OX=3659 GN=ABI2 PE=3 SV=... [more]
A0A0A0KGU32.3e-5096.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G363580 PE=3 SV=1[more]
A0A5A7V9A71.3e-4298.82Protein phosphatase 2C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3BCF61.3e-4298.82uncharacterized protein At4g33100 OS=Cucumis melo OX=3656 GN=LOC103488506 PE=3 S... [more]
A0A6J1BQK53.8e-3787.06uncharacterized protein At4g33100 OS=Momordica charantia OX=3673 GN=LOC111004711... [more]
Match NameE-valueIdentityDescription
AFL56849.15.48e-6994.64protein phosphatase 2C [Cucumis sativus][more]
XP_004144203.16.16e-6896.00uncharacterized protein At4g33100 [Cucumis sativus] >KGN47602.1 hypothetical pro... [more]
XP_008445512.19.09e-5898.82PREDICTED: uncharacterized protein At4g33100 [Cucumis melo] >KAA0064723.1 protei... [more]
XP_038886487.16.61e-5190.59uncharacterized protein At4g33100 [Benincasa hispida][more]
XP_022131556.11.33e-5087.06uncharacterized protein At4g33100 [Momordica charantia] >XP_022131557.1 uncharac... [more]
Match NameE-valueIdentityDescription
AT4G33100.12.1e-2470.59CONTAINS InterPro DOMAIN/s: Mitochondrial distribution/morphology family 35/apop... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007918Mitochondrial distribution/morphology family 35/apoptosisPFAMPF05254UPF0203coord: 51..112
e-value: 1.2E-23
score: 83.1
IPR007918Mitochondrial distribution/morphology family 35/apoptosisPANTHERPTHR46403TP53-REGULATED INHIBITOR OF APOPTOSIS 1coord: 43..122
NoneNo IPR availablePROSITEPS51808CHCHcoord: 53..103
score: 14.080887
IPR036811Ubiquinol-cytochrome C reductase hinge domain superfamilySUPERFAMILY81531Non-heme 11 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)coord: 42..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G056520.1Chy3G056520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004722 protein serine/threonine phosphatase activity