Chy3G055300 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G055300
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	gibberellin 20 oxidase 3
Location	chrH03: 7790010 .. 7790714 (-)
RNA-Seq Expression	Chy3G055300
Synteny	Chy3G055300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCAAATATCTCTCAATTAGAATCCCAAGAGCAAGCACCCAATGAAGCACTTCCTTTTCTCCACGCTTCCAAATTAACCCATAAAACCCTATTCGTACAATCCAAATTTATTTGGCCAAAAGGGGATTTAGTAGAAGCTTATGAAAAGCTAAGTGAACCACATGTGGATCTTGAGGGCTTCCTCAAGGGCGATAAAAAGGCAACCCTTGAAGCTTCCAAGCTTGTGAGGAAAGCCTGCTTGAAACATGGCTTCTTCCAAGTTACTAACCATGGCGTCGATCAAAATCTCTTGGCCACTGCCCTTCACGAAATGGGACCTATTTTTAACCTTCCTTTTAACGTTAAGACGAGGGCCTCTCAATCCCACCCTGGCAAAATGTGGGGCTTTTCTACTGCTCATTCAAATCGCTTTTCTTCAAAGCTGCCATGGAAGGAAACTTTCTCTTTTGGGTTTGACCATTGCAATAGCTTCAATAATGAACCATCTGTTGTTGATTTCTTCTCTTCCACTCTGGGCAAGGAATTTAAAGAAATTGGGTATGGTATTCAAACCCCTATATCTCAAATTAAAAACTAATAAATTTTGGAAAAAAACATGCATCTGTTTGTTGCATGATACATTTTTTTTTTCTTTAGAAGTTGTTTTTATTTTTTATTATATATTTTCAGTGTGATGCAGAAAGATTATACACAATATTGA

mRNA sequence

ATGGATTCAAATATCTCTCAATTAGAATCCCAAGAGCAAGCACCCAATGAAGCACTTCCTTTTCTCCACGCTTCCAAATTAACCCATAAAACCCTATTCGTACAATCCAAATTTATTTGGCCAAAAGGGGATTTAGTAGAAGCTTATGAAAAGCTAAGTGAACCACATGTGGATCTTGAGGGCTTCCTCAAGGGCGATAAAAAGGCAACCCTTGAAGCTTCCAAGCTTGTGAGGAAAGCCTGCTTGAAACATGGCTTCTTCCAAGTTACTAACCATGGCGTCGATCAAAATCTCTTGGCCACTGCCCTTCACGAAATGGGACCTATTTTTAACCTTCCTTTTAACGTTAAGACGAGGGCCTCTCAATCCCACCCTGGCAAAATGTGGGGCTTTTCTACTGCTCATTCAAATCGCTTTTCTTCAAAGCTGCCATGGAAGGAAACTTTCTCTTTTGGGTTTGACCATTGCAATAGCTTCAATAATGAACCATCTGTTGTTGATTTCTTCTCTTCCACTCTGGGCAAGGAATTTAAAGAAATTGGTGTGATGCAGAAAGATTATACACAATATTGA

Coding sequence (CDS)

ATGGATTCAAATATCTCTCAATTAGAATCCCAAGAGCAAGCACCCAATGAAGCACTTCCTTTTCTCCACGCTTCCAAATTAACCCATAAAACCCTATTCGTACAATCCAAATTTATTTGGCCAAAAGGGGATTTAGTAGAAGCTTATGAAAAGCTAAGTGAACCACATGTGGATCTTGAGGGCTTCCTCAAGGGCGATAAAAAGGCAACCCTTGAAGCTTCCAAGCTTGTGAGGAAAGCCTGCTTGAAACATGGCTTCTTCCAAGTTACTAACCATGGCGTCGATCAAAATCTCTTGGCCACTGCCCTTCACGAAATGGGACCTATTTTTAACCTTCCTTTTAACGTTAAGACGAGGGCCTCTCAATCCCACCCTGGCAAAATGTGGGGCTTTTCTACTGCTCATTCAAATCGCTTTTCTTCAAAGCTGCCATGGAAGGAAACTTTCTCTTTTGGGTTTGACCATTGCAATAGCTTCAATAATGAACCATCTGTTGTTGATTTCTTCTCTTCCACTCTGGGCAAGGAATTTAAAGAAATTGGTGTGATGCAGAAAGATTATACACAATATTGA

Protein sequence

MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVMQKDYTQY*

Homology

[Close]

BLAST of Chy3G055300 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 6.2e-28
Identity = 66/157 (42.04%), Postives = 100/157 (63.69%), Query Frame = 0


Query: 34  VQSKFIWPKGD--LVEAYEKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVTN 93
           + S+FIWP+G+    +A E+L  P +D+ G L GD +AT E ++LV +AC +HGFFQV N
Sbjct: 17  IPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVN 76

Query: 94  HGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFSF 153
           HG+D  LLA A   +   F +P   K RA +  PG+  G++++ + RF+SKLPWKET SF
Sbjct: 77  HGIDAELLADAHRCVDAFFTMPLPEKQRALR-RPGESCGYASSFTGRFASKLPWKETLSF 136

Query: 154 GFDHCNSFNNEPS-VVDFFSSTLGKEFKEIGVMQKDY 188
                 S  ++P+ VVD+  +TLG++ + +G +   Y
Sbjct: 137 -----RSCPSDPALVVDYIVATLGEDHRRLGEVYARY 167

[Close]

BLAST of Chy3G055300 vs. ExPASy Swiss-Prot
Match: O04705 (Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 8.1e-28
Identity = 66/157 (42.04%), Postives = 99/157 (63.06%), Query Frame = 0


Query: 34  VQSKFIWPKGD--LVEAYEKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVTN 93
           + S+FIWP+G+    +A E+L  P +D+ G L GD  A  E ++LV +AC +HGFFQV N
Sbjct: 17  IPSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGEACERHGFFQVVN 76

Query: 94  HGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFSF 153
           HG+D  LLA A   +   F +P   K RA + HPG+  G++++ + RF+SKLPWKET SF
Sbjct: 77  HGIDAELLADAHRCVDNFFTMPLPEKQRALR-HPGESCGYASSFTGRFASKLPWKETLSF 136

Query: 154 GFDHCNSFNNEPS-VVDFFSSTLGKEFKEIGVMQKDY 188
                 S  ++P+ VVD+  +TLG++ + +G +   Y
Sbjct: 137 -----RSCPSDPALVVDYIVATLGEDHRRLGEVYARY 167

[Close]

BLAST of Chy3G055300 vs. ExPASy Swiss-Prot
Match: Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 9.0e-27
Identity = 66/180 (36.67%), Postives = 106/180 (58.89%), Query Frame = 0


Query: 11  QEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEA-YEKLSEPHVDLEGFLKGDKKA 70
           Q+   ++  P +    L +    + ++FIWP  +       +L+ P +DL       + +
Sbjct: 19  QDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDS 78

Query: 71  TLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMW 130
           TLEA +++ +AC KHGFF V NHGV ++L+A A   M   F++P   K +A Q  PG+  
Sbjct: 79  TLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKA-QRKPGESC 138

Query: 131 GFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVMQKDYTQ 190
           G++++ + RFS+KLPWKET SF F + NS     +V D+FS TLG+EF++ G + +DY +
Sbjct: 139 GYASSFTGRFSTKLPWKETLSFQFSNDNS--GSRTVQDYFSDTLGQEFEQFGKVYQDYCE 190

[Close]

BLAST of Chy3G055300 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.6e-26
Identity = 64/157 (40.76%), Postives = 98/157 (62.42%), Query Frame = 0


Query: 34  VQSKFIWPKGD--LVEAYEKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVTN 93
           + S+FIWP+G+    +A E+L  P +++ G L GD  A  E ++LV +AC +HGFFQV N
Sbjct: 17  IPSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGFFQVVN 76

Query: 94  HGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFSF 153
           HG+D  LLA A   +   F +P   K RA +  PG+  G++++ + RF+SKLPWKET SF
Sbjct: 77  HGIDAELLADAHRCVDNFFTMPLPEKQRALR-RPGESCGYASSFTGRFASKLPWKETLSF 136

Query: 154 GFDHCNSFNNEPS-VVDFFSSTLGKEFKEIGVMQKDY 188
                 S  ++P+ VVD+  +TLG++ + +G +   Y
Sbjct: 137 -----RSCPSDPALVVDYIVATLGEDHRRLGEVYARY 167

[Close]

BLAST of Chy3G055300 vs. ExPASy Swiss-Prot
Match: P0C5H5 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.3e-25
Identity = 66/157 (42.04%), Postives = 88/157 (56.05%), Query Frame = 0


Query: 34  VQSKFIWPKGDLVEA-YEKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVTNH 93
           +   F+WP GD   A   +L  P VD+     GD +    A+  V  AC  HGFFQV+ H
Sbjct: 42  IPEPFVWPNGDARPASAAELDMPVVDVGVLRDGDAEGLRRAAAQVAAACATHGFFQVSEH 101

Query: 94  GVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFSFG 153
           GVD  L   AL      F LP   K RA +  PG + G+++AH++RF+SKLPWKET SFG
Sbjct: 102 GVDAALARAALDGASDFFRLPLAEKRRARRV-PGTVSGYTSAHADRFASKLPWKETLSFG 161

Query: 154 FDHCNSFNNEPSVVDFFSSTLGKEFKEIGVMQKDYTQ 190
           F   +     P V D+FSSTLG +F  +G + + Y +
Sbjct: 162 F---HDRAAAPVVADYFSSTLGPDFAPMGRVYQKYCE 194

[Close]

BLAST of Chy3G055300 vs. ExPASy TrEMBL
Match: S0BE44 (GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox5 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.0e-101
Identity = 182/189 (96.30%), Postives = 185/189 (97.88%), Query Frame = 0


Query: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60
           MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE
Sbjct: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60

Query: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120
           GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA
Sbjct: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120

Query: 121 SQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI 180
           SQSHP KMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI
Sbjct: 121 SQSHPAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI 180

Query: 181 GVMQKDYTQ 190
           GV+ + Y +
Sbjct: 181 GVIYEKYCE 189

[Close]

BLAST of Chy3G055300 vs. ExPASy TrEMBL
Match: A0A5D3D529 (Gibberellin 20 oxidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold390G00180 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 5.5e-96
Identity = 173/189 (91.53%), Postives = 181/189 (95.77%), Query Frame = 0


Query: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60
           MDSNIS+L+SQEQAPNEALPFLHASKLTHKTLFV SKFIWPKGD+VEAY KLSEP+VDLE
Sbjct: 1   MDSNISELDSQEQAPNEALPFLHASKLTHKTLFVPSKFIWPKGDIVEAYGKLSEPYVDLE 60

Query: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120
           GFLKGDKKATLEASKLVRKACL+HGFFQVTNHGVDQNLLATAL EMGPIFNLPFNVKTRA
Sbjct: 61  GFLKGDKKATLEASKLVRKACLEHGFFQVTNHGVDQNLLATALLEMGPIFNLPFNVKTRA 120

Query: 121 SQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI 180
           SQSHP KMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSS LGKEFKEI
Sbjct: 121 SQSHPAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSALGKEFKEI 180

Query: 181 GVMQKDYTQ 190
           GV+ + Y +
Sbjct: 181 GVIYEKYCE 189

[Close]

BLAST of Chy3G055300 vs. ExPASy TrEMBL
Match: A0A1S3B3S2 (gibberellin 20 oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103485678 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 5.5e-96
Identity = 173/189 (91.53%), Postives = 181/189 (95.77%), Query Frame = 0


Query: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60
           MDSNIS+L+SQEQAPNEALPFLHASKLTHKTLFV SKFIWPKGD+VEAY KLSEP+VDLE
Sbjct: 1   MDSNISELDSQEQAPNEALPFLHASKLTHKTLFVPSKFIWPKGDIVEAYGKLSEPYVDLE 60

Query: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120
           GFLKGDKKATLEASKLVRKACL+HGFFQVTNHGVDQNLLATAL EMGPIFNLPFNVKTRA
Sbjct: 61  GFLKGDKKATLEASKLVRKACLEHGFFQVTNHGVDQNLLATALLEMGPIFNLPFNVKTRA 120

Query: 121 SQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI 180
           SQSHP KMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSS LGKEFKEI
Sbjct: 121 SQSHPAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSALGKEFKEI 180

Query: 181 GVMQKDYTQ 190
           GV+ + Y +
Sbjct: 181 GVIYEKYCE 189

[Close]

BLAST of Chy3G055300 vs. ExPASy TrEMBL
Match: A0A6J1CIQ5 (gibberellin 20 oxidase 3-like OS=Momordica charantia OX=3673 GN=LOC111011962 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.3e-65
Identity = 129/187 (68.98%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 3   SNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLEGF 62
           S+ SQLE Q+Q P+  L FL  S+ ++KTL + SKFIWPKGDLV+A+ KL+EP+VDL+GF
Sbjct: 11  SSQSQLEPQDQRPSTQLSFLDNSQ-SNKTLLLPSKFIWPKGDLVDAHHKLTEPYVDLQGF 70

Query: 63  LKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRASQ 122
           LKGDK AT+ AS LVRKAC+KHGFFQV NHGVD  LLATAL +MG +FNLP N+KTRAS 
Sbjct: 71  LKGDKNATIAASNLVRKACMKHGFFQVVNHGVDHTLLATALDKMGSLFNLPSNLKTRAS- 130

Query: 123 SHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGV 182
               KMWGFSTAHSNRFSSKLPWKETFSFGFDHC + +N+PSV  FF+STLGKEF+EIGV
Sbjct: 131 ----KMWGFSTAHSNRFSSKLPWKETFSFGFDHCIT-SNDPSVAHFFTSTLGKEFEEIGV 190

Query: 183 MQKDYTQ 190
           + + Y +
Sbjct: 191 VYQKYCE 190

[Close]

BLAST of Chy3G055300 vs. ExPASy TrEMBL
Match: A0A6J1FF43 (gibberellin 20 oxidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111445146 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.2e-61
Identity = 119/159 (74.84%), Postives = 134/159 (84.28%), Query Frame = 0


Query: 32  LFVQSKFIWPKGDLVEAY-EKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVT 91
           L + S+FIWPK DLV+AY EKLSEPHVDL GFLKGD+KAT+EAS LVR AC+KHGFFQV 
Sbjct: 8   LVLSSQFIWPKEDLVDAYDEKLSEPHVDLHGFLKGDQKATIEASHLVRTACMKHGFFQVI 67

Query: 92  NHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFS 151
           NHGVDQ LLATAL +MGP+FNLPF++KT AS     KMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 68  NHGVDQTLLATALDKMGPLFNLPFHLKTTAS----AKMWGFSTAHSNRFSSKLPWKETFS 127

Query: 152 FGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVMQKDYTQ 190
           FGFDHC + NNEP VVDFFSS LG EF+EIG + + Y +
Sbjct: 128 FGFDHCKTSNNEPLVVDFFSSILGHEFQEIGALYEKYCE 162

[Close]

BLAST of Chy3G055300 vs. NCBI nr
Match: XP_004144295.2 (gibberellin 20 oxidase 1-D [Cucumis sativus] >KGN47512.1 hypothetical protein Csa_018977 [Cucumis sativus] >CCG14216.1 GA 20-oxidase [Cucumis sativus])

HSP 1 Score: 376 bits (966), Expect = 1.25e-128
Identity = 182/189 (96.30%), Postives = 185/189 (97.88%), Query Frame = 0


Query: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60
           MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE
Sbjct: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60

Query: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120
           GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA
Sbjct: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120

Query: 121 SQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI 180
           SQSHP KMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI
Sbjct: 121 SQSHPAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI 180

Query: 181 GVMQKDYTQ 189
           GV+ + Y +
Sbjct: 181 GVIYEKYCE 189

[Close]

BLAST of Chy3G055300 vs. NCBI nr
Match: XP_008441598.1 (PREDICTED: gibberellin 20 oxidase 3 [Cucumis melo] >TYK18655.1 gibberellin 20 oxidase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 358 bits (919), Expect = 1.74e-121
Identity = 173/189 (91.53%), Postives = 181/189 (95.77%), Query Frame = 0


Query: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60
           MDSNIS+L+SQEQAPNEALPFLHASKLTHKTLFV SKFIWPKGD+VEAY KLSEP+VDLE
Sbjct: 1   MDSNISELDSQEQAPNEALPFLHASKLTHKTLFVPSKFIWPKGDIVEAYGKLSEPYVDLE 60

Query: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120
           GFLKGDKKATLEASKLVRKACL+HGFFQVTNHGVDQNLLATAL EMGPIFNLPFNVKTRA
Sbjct: 61  GFLKGDKKATLEASKLVRKACLEHGFFQVTNHGVDQNLLATALLEMGPIFNLPFNVKTRA 120

Query: 121 SQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEI 180
           SQSHP KMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSS LGKEFKEI
Sbjct: 121 SQSHPAKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSALGKEFKEI 180

Query: 181 GVMQKDYTQ 189
           GV+ + Y +
Sbjct: 181 GVIYEKYCE 189

[Close]

BLAST of Chy3G055300 vs. NCBI nr
Match: XP_038885915.1 (gibberellin 20 oxidase 3-like [Benincasa hispida])

HSP 1 Score: 318 bits (814), Expect = 1.55e-105
Identity = 154/190 (81.05%), Postives = 171/190 (90.00%), Query Frame = 0


Query: 1   MDSNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLE 60
           MDS+ SQLESQEQA N+ LPFL  S+LTHKTLF+ SKFIWPKGDLV+AYEKLSEPH+DLE
Sbjct: 1   MDSSSSQLESQEQALNQQLPFLDTSQLTHKTLFIPSKFIWPKGDLVDAYEKLSEPHIDLE 60

Query: 61  GFLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRA 120
           GFLKGDK+AT+EASKLVRKACLKHGFFQVTNHGVDQNLLA AL +MG +FNLPFN+K+RA
Sbjct: 61  GFLKGDKEATIEASKLVRKACLKHGFFQVTNHGVDQNLLAIALDKMGTLFNLPFNLKSRA 120

Query: 121 SQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDH-CNSFNNEPSVVDFFSSTLGKEFKE 180
           S +   KMWGFSTAHSNRFSSKLPWKETFSFGFDH CNS NNEP VVDFFSSTLGKEF+E
Sbjct: 121 SHARAAKMWGFSTAHSNRFSSKLPWKETFSFGFDHSCNSSNNEPPVVDFFSSTLGKEFEE 180

Query: 181 IGVMQKDYTQ 189
           IGV+ + Y +
Sbjct: 181 IGVIYEKYCE 190

[Close]

BLAST of Chy3G055300 vs. NCBI nr
Match: XP_022141660.1 (gibberellin 20 oxidase 3-like [Momordica charantia])

HSP 1 Score: 256 bits (655), Expect = 2.05e-81
Identity = 129/187 (68.98%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 3   SNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEAYEKLSEPHVDLEGF 62
           S+ SQLE Q+Q P+  L FL  S+ ++KTL + SKFIWPKGDLV+A+ KL+EP+VDL+GF
Sbjct: 11  SSQSQLEPQDQRPSTQLSFLDNSQ-SNKTLLLPSKFIWPKGDLVDAHHKLTEPYVDLQGF 70

Query: 63  LKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRASQ 122
           LKGDK AT+ AS LVRKAC+KHGFFQV NHGVD  LLATAL +MG +FNLP N+KTRAS 
Sbjct: 71  LKGDKNATIAASNLVRKACMKHGFFQVVNHGVDHTLLATALDKMGSLFNLPSNLKTRAS- 130

Query: 123 SHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGV 182
               KMWGFSTAHSNRFSSKLPWKETFSFGFDHC + +N+PSV  FF+STLGKEF+EIGV
Sbjct: 131 ----KMWGFSTAHSNRFSSKLPWKETFSFGFDHCIT-SNDPSVAHFFTSTLGKEFEEIGV 190

Query: 183 MQKDYTQ 189
           + + Y +
Sbjct: 191 VYQKYCE 190

[Close]

BLAST of Chy3G055300 vs. NCBI nr
Match: XP_023550661.1 (gibberellin 20 oxidase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245 bits (626), Expect = 1.85e-77
Identity = 120/159 (75.47%), Postives = 135/159 (84.91%), Query Frame = 0


Query: 32  LFVQSKFIWPKGDLVEAY-EKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVT 91
           L + S+FIWPK DLV+AY EKLSEPHVDL GFLKGD+KAT+EAS +VR AC+KHGFFQV 
Sbjct: 8   LVLSSQFIWPKEDLVDAYYEKLSEPHVDLHGFLKGDQKATIEASHIVRTACMKHGFFQVI 67

Query: 92  NHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFS 151
           NHGVDQ+LLATAL +MGP+FNLPFN+KT AS     KMWGFSTAHSNRFSSKLPWKETFS
Sbjct: 68  NHGVDQSLLATALDKMGPLFNLPFNLKTTAST----KMWGFSTAHSNRFSSKLPWKETFS 127

Query: 152 FGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVMQKDYTQ 189
           FGFDHC S NNEP VVDFFSS LG EF+EIG + + Y +
Sbjct: 128 FGFDHCKSSNNEPLVVDFFSSMLGHEFQEIGALYEKYCE 162

[Close]

BLAST of Chy3G055300 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 121.7 bits (304), Expect = 6.4e-28
Identity = 66/180 (36.67%), Postives = 106/180 (58.89%), Query Frame = 0


Query: 11  QEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEA-YEKLSEPHVDLEGFLKGDKKA 70
           Q+   ++  P +    L +    + ++FIWP  +       +L+ P +DL       + +
Sbjct: 19  QDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIPELNVPFIDL-----SSQDS 78

Query: 71  TLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMW 130
           TLEA +++ +AC KHGFF V NHGV ++L+A A   M   F++P   K +A Q  PG+  
Sbjct: 79  TLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKA-QRKPGESC 138

Query: 131 GFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVMQKDYTQ 190
           G++++ + RFS+KLPWKET SF F + NS     +V D+FS TLG+EF++ G + +DY +
Sbjct: 139 GYASSFTGRFSTKLPWKETLSFQFSNDNS--GSRTVQDYFSDTLGQEFEQFGKVYQDYCE 190

[Close]

BLAST of Chy3G055300 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 115.2 bits (287), Expect = 6.0e-26
Identity = 66/188 (35.11%), Postives = 104/188 (55.32%), Query Frame = 0


Query: 3   SNISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDLVEA-YEKLSEPHVDLEG 62
           + + Q+ S+ +   ++  F  A  L   +  +  +F+WP  +      + L  P +DL G
Sbjct: 7   ATVPQIFSENKTKEDSSIF-DAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPLIDLAG 66

Query: 63  FLKGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRAS 122
           FL GD     EA++LV KA  KHGFF +TNHGVD++LL+ A   M   F  P   K +A 
Sbjct: 67  FLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACEKQKA- 126

Query: 123 QSHPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEIG 182
           Q   G+  G++++   RFSSKLPWKET SF F      +++ +V DF S  +G  +++ G
Sbjct: 127 QRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQ-TVKDFVSKKMGDGYEDFG 186

Query: 183 VMQKDYTQ 190
            + ++Y +
Sbjct: 187 KVYQEYAE 191

[Close]

BLAST of Chy3G055300 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 111.7 bits (278), Expect = 6.6e-25
Identity = 59/175 (33.71%), Postives = 97/175 (55.43%), Query Frame = 0


Query: 16  NEALPFLHASKLTHKTLFVQSKFIWPKGDLVEA-YEKLSEPHVDLEGFLKGDKKATLEAS 75
           N   P +    + +    + ++FIWP  +       +L  P +DL+  L  D  +TL+AS
Sbjct: 22  NIQTPLIFNPSMLNLQANIPNQFIWPDDEKPSINVLELDVPLIDLQNLL-SDPSSTLDAS 81

Query: 76  KLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTA 135
           +L+ +AC KHGFF V NHG+ + L++ A       F++P + K R  +   G+  G++++
Sbjct: 82  RLISEACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRK-SGESVGYASS 141

Query: 136 HSNRFSSKLPWKETFSFGFDHCNSFNNEPSVVDFFSSTLGKEFKEIGVMQKDYTQ 190
            + RFS+KLPWKET SF F  C+  +   SV D+F   LG  F+  G + ++Y +
Sbjct: 142 FTGRFSTKLPWKETLSFRF--CDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCE 192

[Close]

BLAST of Chy3G055300 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 110.2 bits (274), Expect = 1.9e-24
Identity = 63/187 (33.69%), Postives = 98/187 (52.41%), Query Frame = 0


Query: 5   ISQLESQEQAPNEALPFLHASKLTHKTLFVQSKFIWPKGDL-VEAYEKLSEPHVDLEGFL 64
           + Q  ++ ++    L    ++ L H+   +  +F+WP  +   +    L  P +DL GFL
Sbjct: 7   LPQRFNKNKSKKNPLRIFDSTVLNHQPDHIPQEFVWPDHEKPSKNVPILQVPVIDLAGFL 66

Query: 65  KGDKKATLEASKLVRKACLKHGFFQVTNHGVDQNLLATALHEMGPIFNLPFNVKTRASQS 124
             D     EA +LV +A  KHGFF VTNHGVD+ LL+TA   M   F  P N +   +Q 
Sbjct: 67  SNDPLLVSEAERLVSEAAKKHGFFLVTNHGVDERLLSTAHKLMDTFFKSP-NYEKLKAQR 126

Query: 125 HPGKMWGFSTAHSNRFSSKLPWKETFSFGFDHC-NSFNNEPSVVDFFSSTLGKEFKEIGV 184
             G+  G++++   RF   LPWKET SF F     S N   +V ++ S T+G  +K+ G 
Sbjct: 127 KVGETTGYASSFVGRFKENLPWKETLSFSFSPTEKSENYSQTVKNYISKTMGDGYKDFGS 186

Query: 185 MQKDYTQ 190
           + ++Y +
Sbjct: 187 VYQEYAE 192

[Close]

BLAST of Chy3G055300 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-23
Identity = 61/155 (39.35%), Postives = 84/155 (54.19%), Query Frame = 0


Query: 34  VQSKFIWPKGDLVEAYEKLSEPHVDLEGFLKGDKKATLEASKLVRKACLKHGFFQVTNHG 93
           V ++F WP+ D+  +   L  P +DL GFL G++  T  A+K V+KAC+ HG F V NHG
Sbjct: 43  VPAEFFWPEKDVAPSEGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLVVNHG 102

Query: 94  VDQNLLATALHEMGPIFNLPFNVKTRASQSHPGKMWGFSTAHSNRFSSKLPWKETFSFGF 153
               L   AL      F L  + K RA +  PG + G++  HS RFSS LPW ET +  F
Sbjct: 103 FKSGLAEKALEISSLFFGLSKDEKLRAYRI-PGNISGYTAGHSQRFSSNLPWNETLTLAF 162

Query: 154 DHCNSFNNEPSVV-DFFSSTLGKEFKEIGVMQKDY 188
                    P VV DF +S LG   +EIG + +++
Sbjct: 163 K-----KGPPHVVEDFLTSRLGNHRQEIGQVFQEF 191

The following BLAST results are available for this feature:

BLAST of Chy3G055300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04706	6.2e-28	42.04	Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1	[more]
O04705	8.1e-28	42.04	Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1	[more]
Q39111	9.0e-27	36.67	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]
O04707	2.6e-26	40.76	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]
P0C5H5	1.3e-25	42.04	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV...	[more]

BLAST of Chy3G055300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
S0BE44	2.0e-101	96.30	GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox5 PE=2 SV=1	[more]
A0A5D3D529	5.5e-96	91.53	Gibberellin 20 oxidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3B3S2	5.5e-96	91.53	gibberellin 20 oxidase 3 OS=Cucumis melo OX=3656 GN=LOC103485678 PE=3 SV=1	[more]
A0A6J1CIQ5	2.3e-65	68.98	gibberellin 20 oxidase 3-like OS=Momordica charantia OX=3673 GN=LOC111011962 PE=...	[more]
A0A6J1FF43	1.2e-61	74.84	gibberellin 20 oxidase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111445146 PE=3...	[more]

BLAST of Chy3G055300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144295.2	1.25e-128	96.30	gibberellin 20 oxidase 1-D [Cucumis sativus] >KGN47512.1 hypothetical protein Cs...	[more]
XP_008441598.1	1.74e-121	91.53	PREDICTED: gibberellin 20 oxidase 3 [Cucumis melo] >TYK18655.1 gibberellin 20 ox...	[more]
XP_038885915.1	1.55e-105	81.05	gibberellin 20 oxidase 3-like [Benincasa hispida]	[more]
XP_022141660.1	2.05e-81	68.98	gibberellin 20 oxidase 3-like [Momordica charantia]	[more]
XP_023550661.1	1.85e-77	75.47	gibberellin 20 oxidase 3-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy3G055300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G51810.1	6.4e-28	36.67	gibberellin 20 oxidase 2	[more]
AT5G07200.1	6.0e-26	35.11	gibberellin 20-oxidase 3	[more]
AT4G25420.1	6.6e-25	33.71	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G60980.1	1.9e-24	33.69	gibberellin 20-oxidase 4	[more]
AT1G44090.1	1.2e-23	39.35	gibberellin 20-oxidase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 55..155 e-value: 3.6E-19 score: 69.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 24..185 e-value: 2.4E-21 score: 78.3
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 31..189
None	No IPR available	PANTHER	PTHR47990:SF86	GIBBERELLIN 20 OXIDASE 5	coord: 31..189
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 35..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G055300.1	Chy3G055300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009908	flower development
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
biological_process	GO:0009826	unidimensional cell growth
molecular_function	GO:0045544	gibberellin 20-oxidase activity
molecular_function	GO:0046872	metal ion binding