Chy3G050400 (gene) Cucumber (hystrix) v1

Overview
NameChy3G050400
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein disulfide-isomerase 5-2-like
LocationchrH03: 610559 .. 615292 (-)
RNA-Seq ExpressionChy3G050400
SyntenyChy3G050400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGAGATCCTCGCAATTTCTGGTTCTTTTCCTCCTTTCAATCTCATCACTGCCATCCTTTTCCTTTTCCACCTCGGAGCTGCCGCTCACGGCGGATGGAAAAGTGTTGGACCTCGACGATTCCAATTTCGATCTCGCCATTTCATCATTTGATTATATATTGGTCGATTTTTACGCCCCATGGTGCGGCCACTGCAAGCGTCTTTCTCCAGAGGTTCCTTGTTTTCTCTTCCGTTCTTTTTAGATTATCTTGCGTATTCCCACTCTTAATTCTATATTTCTTCCAGGTTATTTTATCTCGTCAAAGGAAAATTTGTTAAAGCCTTAGTATGAAAGTGTAGAGTGAAGTAAGCGGAATCGATGGAGTGTTGAATTACTTATACTTGTAATAGAATCAGTTCGTTAATACTATGGAGGACTTAAACACAAATATGGTTTTAGATAGAACGACAATGAGGATATTGAAAATATGAATCTTCTACGTCGGCTTCATATATTTGGAGCAACTGAAGTCGGTTCATTATTTATTATTATAGGGGATTATTACTTTTGAGAGTTTTTCATCTGTTATTTGCTGATTCTAATTGATGAATCTGTCACCTTTTTTTTTAAACTATTAGTTTACTAGATGATTGATCCTTCTACTTCGTGCTCCACCATCCGTACACCAATTCCATCACATATTCTTTGAGTTATTATTGTTATTTTTTTAATTAATTATTATTGTTGTTGTTTTTTGTGACCTGATTTCAGCTTTGGAAGAGGCAAGAAATGTAAAGAAATTTCGGTGCTGGAATCTGGATTGTGTTCATGATATTCTTTAGGGGGGTTCTCTTCGTTCTTATTTAGATCATAATGGGTCTTGGTGGCTAAATCCATCTGTTGTTATCTCCTTGCCCAGTTAATTTCTTTTTATCTTTGAAGAACATTTGAAAGAGGAAGGTTAAGATGGTTGTCCAGATGGAGGATTAACTCTAATGTTAATTCCTAGAGGAGGAATTCATTTATGTACCTGCATACCTACAACCTCTTTGGTGTATTCTTTGTATGAGTTTTAGCATATAACTTATAAGAGCTGTTGTTTGGTTCAGTCTAGTTCCCCACAGTTTCTGGATTAGTTCTTCTGTCTTGAGGTGTGAGCTTCATTTCAAATGAGTGTTGTGGATTTTATTTTAATGTTAGAGACATGGTCCAACCCGGGAGGAGGTATGGTAGGTATTATGTAGTATGGATTTGGAACTGAATGTTTGAAGGCGAAGGTGCGTTAGCTGTCTCGTTGTCTGATAGATAAGTATCCGCCCAAGTTCCACAGTTCACATGTTGCCTGTAAGTAAATGCGGGACAGCTAGGGGTAAAGCACTACTGGTACTGGAGTTCAGGTGAGAGCTTAGCTATAGTAGCGCTTAACATCTATAGTAGTTAAAGAGTGTTGTCATAGTTTAGTTTCATTTTCTTACACCCAGCCTTTGAATATAGTCTGTAGAACTCAAGCTATCGACTGAAGTGAAACCCCACGAACGATAGTTCACTACGATAGTAGTGGGACTGTAATGCATGTGTGATTCTGCTCATAGGGATTGTACCTAACTTCTTCAAGTACTTCCTCACAGTTGTCGCATTTGCTTTCTCTCTGAACAAAGGACTGTGGCTTCTTTCTTCTCTATCTATCTAAAAGTAGAGTCACTGGTCAGCAGAGCATTTTTATGGCTTTCATAGCGTTTAGTAGTTCTAGGGATAAGTAGCTACAAGAATCTACTGAGCTAACCATCTAATCTAATCAGTAGCATCTCAACTCAAGGTCTTTTATGCCCGCGAGAGGAAAGTGATAAGAAAAGTCTCTATCTACACGAAACACACAACCACAACAATCAAATAGAAAGACTACGGCTAAAGAAATAATATTTGAAGGAGTTTTTGCTAAGTTGGCTCTTACTTCGTATGGAGGGAGATGGTTCTTGCCTTCTTGGAAGGAGTGCAAGTTTCTTTAATAAATTTATTATGCATCAAAGACTCCAGTTTGCAGATTTTGACAATTTGCTTTGGGATACCATCACTAGTAAGCTTCAAAATCTTTGATTGTCAGCAATCAGGAGGCTTACTTGTAAAGTTCTTTACAGGTTTTTTTGTTGTCGCTCTGATTGGTTTGTTTTTGGACTGCAGTTGTTATTCTCCAGTTTTTCTATTGTATTTGATTTGTCATGTTTTTTAATGTGATGTATGCCATTTGAGTCGAAATACAGATATTTCGCCCAAAAGCGTAGTCTATTATGCACAGAGTATGCATTCTTTTCTTCTCTTAATTTTTTTTCTTCATTTGTGTAGTTGGACGCTGCTGCACCTCAGCTTGCCAGATTGAAGGAGCCTATAGTTCTAGCAAAAGTGAATGCTGACAAGTATACTAGTCTTGCTAAAAAATATGACGTTGAGTATGCCACTGACCCTGATTCATATCTCCAATGTAATAAACTATAGGTTTATTTGTATTAAGTTTGGAGTTTAGTTGTGCCCTTTGGCTTAAACGTAGAATTTAAGTACGAATCTCCATTAGACGATTTCCCTGCTCAATAAGCTTGCTAGTGAATACCAGTTCTTGAATTATATGAAGGGTAAACCATGGTTTTCTTCTACCTATGTTAACCTTTTGTTTTCTTTTTGGTAGTGCCTATCCTACAATCAAGATCTTCATGCATGGTGTCCCAGTAGACTATTATGGACCAAGGAAAGCAGAGTTACTTGCCCGTTATCTGAAGAAATTTGTTGCTCCAGATGTCTCAGTTCTTGAGTCAGACTCATCAATTAATGAATTTGTTGAAGCTGCTGGGCCTTACTTCCCTATCTATTTGGGTTTTGGTTTAGATGAATCTGTTATTTCTAAATTCGGTATTAAGTATAAGAAAAAGGCCTGGTTTTCGGTGGCAAAGGGTTTCTCAGAGGATATCATGGTGTCTTATGATTTTGATAAAGTTCCTGCTTTGGTTTCTATCCATCCAAATTACAACAATGAGCGGAGCATCTTTTATGGTCCTTTTGAAGGTTGGTTTGTATACTATACTACACCTAAACTGTTTAATGTAGCATTTTCTTAAGTTCTTCAATCTGGCATATCTGGCATTAGAAGTTTACATTCTTTTGAATGTTTCATGCACTGTTGATAGGAATGTTAGACTGCATAAGATTCATTTTGCACTGGCGAGTCACTTGTTGAAAGAATTCGAAACCAGAGGTTTTTTATATTTGTTATGAACTCCTAAAGAGGCTATGTTTCCAATTGCTTGAATTCGAACCTGTTGCTTGTTTACACCATATGTTTCAGCTTGAGTTCTCATCCATTTTCAAGAACATCTCAACAACATTTCTTGTCGTGTATGTAGTACTTCAGGTTCATGAACATCCAACCGTATGGCTCTATGTATTCTATTTTAAATGGTTTAAGCCTTTCTGTTTGAATTGTACAGAACAATTCTTGGAGGAATTCATAAAACAAAGTCTGTTCCCTCTTGTTCTGCCGATAAACTACGACACTCTTAAGCTACTAAAAGACGATGACAGGAAAATAGCACTCACAATTGTGGAGGATGAGGATGAGGACCAGACTAAGAAGTTGATCAATTTATTAAAGGCTGCTGCATCTGCTAATCGTGACTTGGTGTTTGCTTACGTTGGGGCTAAACAGTGGGGAGAATTTGCCGATTCGTTTGGAGACAAGAAGACTACACTGCCAAAAATGGTCATTTGGGACGGGGAGGACGATTACTTAATGGTAAGTTCTCATTAAATGCAAATTAAGCATATTATACTCGTTAAATCTTTTAGACATGCATGTGATAAAATGAAATATTAACTCATGCATGTATCTATTTCAAAGAAACAAGTTCTCTTTAATAGGTTTAATTATTTTAAAAGAATTATTCACTCAATTAGTTCCACTTAATGATGCAGCAAGATGATTTTATCTCAAATATGATCCTTCGGCCAAAAAGGCAGTATGATCTTTTTCCCTCTAAAAAAGATTAACATTTGTAGTTCATGGAAACAACTCAACTACCATTGAAGTTTGCACATTTATTAAGGAACTTTATCGAGAAACTTTATCTTCTCCTCTATGGCTTAACATTTATCAAGTAATTTCATCTTTCCTCTAAATCTACTTTTTACAATTATCGAGAAACTCTATCTTACAACTCTCCACCTCAAACACAAACTTACTTACTCCAGCATATTAACTTCAACTTAAGAACTCCACCCAGTGGGCCAAACACCCTCTAAACGATTGTTTGATTGTTGATTAAAGTATCCAAATGTCTACAATCGCATGCTTGCTATGAAGTTGTCTCTAGAATTAGAAAACTGTTACTTTTTCTTAGCATATGCATTTCATTTCATTTCTACTATTGTGTTTTTATAGGTTACTGGGTCAGAAAGCATCGTCGGGAACGACCATGCATCCGAAATCTCAAAATTTATTGAAGGATACAGAGAAGGAAGAACTGAAAAAAGAAGAGTTGCGGGTCCTGCAATTCTTGGCTTCATAAATTCACTAATAGGCATCAGGACGATATACATCATTGTAATTATTGTTGCAGGAATAATGCTTTACCAAAACCTCACCAAAGATGATAGCGAATATAACAGAGTCGATACTTCTGCTCGGGATCAGGTTGAGCAAACCAGCAGCAGCTCTGCCGCAGAGGTTGAAAGGTCTGAATACAAGGCTGGTGACAAGGAAGATTGA

mRNA sequence

ATGCGGAGATCCTCGCAATTTCTGGTTCTTTTCCTCCTTTCAATCTCATCACTGCCATCCTTTTCCTTTTCCACCTCGGAGCTGCCGCTCACGGCGGATGGAAAAGTGTTGGACCTCGACGATTCCAATTTCGATCTCGCCATTTCATCATTTGATTATATATTGGTCGATTTTTACGCCCCATGGTGCGGCCACTGCAAGCGTCTTTCTCCAGAGTTGGACGCTGCTGCACCTCAGCTTGCCAGATTGAAGGAGCCTATAGTTCTAGCAAAAGTGAATGCTGACAAGTATACTAGTCTTGCTAAAAAATATGACGTTGATGCCTATCCTACAATCAAGATCTTCATGCATGGTGTCCCAGTAGACTATTATGGACCAAGGAAAGCAGAGTTACTTGCCCGTTATCTGAAGAAATTTGTTGCTCCAGATGTCTCAGTTCTTGAGTCAGACTCATCAATTAATGAATTTGTTGAAGCTGCTGGGCCTTACTTCCCTATCTATTTGGGTTTTGGTTTAGATGAATCTGTTATTTCTAAATTCGGTATTAAGTATAAGAAAAAGGCCTGGTTTTCGGTGGCAAAGGGTTTCTCAGAGGATATCATGGTGTCTTATGATTTTGATAAAGTTCCTGCTTTGGTTTCTATCCATCCAAATTACAACAATGAGCGGAGCATCTTTTATGGTCCTTTTGAAGAACAATTCTTGGAGGAATTCATAAAACAAAGTCTGTTCCCTCTTGTTCTGCCGATAAACTACGACACTCTTAAGCTACTAAAAGACGATGACAGGAAAATAGCACTCACAATTGTGGAGGATGAGGATGAGGACCAGACTAAGAAGTTGATCAATTTATTAAAGGCTGCTGCATCTGCTAATCGTGACTTGGTGTTTGCTTACGTTGGGGCTAAACAGTGGGGAGAATTTGCCGATTCGTTTGGAGACAAGAAGACTACACTGCCAAAAATGGTCATTTGGGACGGGGAGGACGATTACTTAATGGTTACTGGGTCAGAAAGCATCGTCGGGAACGACCATGCATCCGAAATCTCAAAATTTATTGAAGGATACAGAGAAGGAAGAACTGAAAAAAGAAGAGTTGCGGGTCCTGCAATTCTTGGCTTCATAAATTCACTAATAGGCATCAGGACGATATACATCATTGTAATTATTGTTGCAGGAATAATGCTTTACCAAAACCTCACCAAAGATGATAGCGAATATAACAGAGTCGATACTTCTGCTCGGGATCAGGTTGAGCAAACCAGCAGCAGCTCTGCCGCAGAGGTTGAAAGGTCTGAATACAAGGCTGGTGACAAGGAAGATTGA

Coding sequence (CDS)

ATGCGGAGATCCTCGCAATTTCTGGTTCTTTTCCTCCTTTCAATCTCATCACTGCCATCCTTTTCCTTTTCCACCTCGGAGCTGCCGCTCACGGCGGATGGAAAAGTGTTGGACCTCGACGATTCCAATTTCGATCTCGCCATTTCATCATTTGATTATATATTGGTCGATTTTTACGCCCCATGGTGCGGCCACTGCAAGCGTCTTTCTCCAGAGTTGGACGCTGCTGCACCTCAGCTTGCCAGATTGAAGGAGCCTATAGTTCTAGCAAAAGTGAATGCTGACAAGTATACTAGTCTTGCTAAAAAATATGACGTTGATGCCTATCCTACAATCAAGATCTTCATGCATGGTGTCCCAGTAGACTATTATGGACCAAGGAAAGCAGAGTTACTTGCCCGTTATCTGAAGAAATTTGTTGCTCCAGATGTCTCAGTTCTTGAGTCAGACTCATCAATTAATGAATTTGTTGAAGCTGCTGGGCCTTACTTCCCTATCTATTTGGGTTTTGGTTTAGATGAATCTGTTATTTCTAAATTCGGTATTAAGTATAAGAAAAAGGCCTGGTTTTCGGTGGCAAAGGGTTTCTCAGAGGATATCATGGTGTCTTATGATTTTGATAAAGTTCCTGCTTTGGTTTCTATCCATCCAAATTACAACAATGAGCGGAGCATCTTTTATGGTCCTTTTGAAGAACAATTCTTGGAGGAATTCATAAAACAAAGTCTGTTCCCTCTTGTTCTGCCGATAAACTACGACACTCTTAAGCTACTAAAAGACGATGACAGGAAAATAGCACTCACAATTGTGGAGGATGAGGATGAGGACCAGACTAAGAAGTTGATCAATTTATTAAAGGCTGCTGCATCTGCTAATCGTGACTTGGTGTTTGCTTACGTTGGGGCTAAACAGTGGGGAGAATTTGCCGATTCGTTTGGAGACAAGAAGACTACACTGCCAAAAATGGTCATTTGGGACGGGGAGGACGATTACTTAATGGTTACTGGGTCAGAAAGCATCGTCGGGAACGACCATGCATCCGAAATCTCAAAATTTATTGAAGGATACAGAGAAGGAAGAACTGAAAAAAGAAGAGTTGCGGGTCCTGCAATTCTTGGCTTCATAAATTCACTAATAGGCATCAGGACGATATACATCATTGTAATTATTGTTGCAGGAATAATGCTTTACCAAAACCTCACCAAAGATGATAGCGAATATAACAGAGTCGATACTTCTGCTCGGGATCAGGTTGAGCAAACCAGCAGCAGCTCTGCCGCAGAGGTTGAAAGGTCTGAATACAAGGCTGGTGACAAGGAAGATTGA

Protein sequence

MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVPVDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKFGIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIKQSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYVGAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGRTEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQTSSSSAAEVERSEYKAGDKED*
Homology
BLAST of Chy3G050400 vs. ExPASy Swiss-Prot
Match: Q94F09 (Protein disulfide-isomerase 5-2 OS=Arabidopsis thaliana OX=3702 GN=PDIL5-2 PE=1 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.5e-149
Identity = 267/437 (61.10%), Postives = 350/437 (80.09%), Query Frame = 0

Query: 8   LVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYAPWCGHCK 67
           L+L  +S  +L     ++S+   T DG VL+L DSNFD AIS+FD I VDFYAPWCGHCK
Sbjct: 6   LLLCWISFLTLSISISASSDDQFTLDGTVLELTDSNFDSAISTFDCIFVDFYAPWCGHCK 65

Query: 68  RLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVPVDYYGPR 127
           RL+PELDAAAP LA+LK+PIV+AK+NADKY+ LA+K ++DA+PT+ ++ HGVP++YYGPR
Sbjct: 66  RLNPELDAAAPILAKLKQPIVIAKLNADKYSRLARKIEIDAFPTLMLYNHGVPMEYYGPR 125

Query: 128 KAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKFGIKYKKK 187
           KA+LL RYLKKFVAPDV+VLESDS++ EFVE AG +FP+++GFGL+ES+IS  G KYKKK
Sbjct: 126 KADLLVRYLKKFVAPDVAVLESDSTVKEFVEDAGTFFPVFIGFGLNESIISGLGRKYKKK 185

Query: 188 AWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIKQSLFPLV 247
           AWF+V+K  SED MVSYDFDK PALV+ HP Y NE S+FYGPFE+ FLEEF+KQS  PL+
Sbjct: 186 AWFAVSKEVSEDTMVSYDFDKAPALVANHPTY-NEHSVFYGPFEDGFLEEFVKQSFLPLI 245

Query: 248 LPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYVGAKQWGE 307
           LPIN+DTLKLLKDD+RKI LTIVEDE  +  +KL   L+AAA ANRDLVF YVG KQ+ E
Sbjct: 246 LPINHDTLKLLKDDERKIVLTIVEDETHESLEKLYKALRAAAHANRDLVFGYVGVKQFEE 305

Query: 308 FADSFG-DKKTTLPKMVIWDGEDDYLMVTGSESIV-GNDHASEISKFIEGYREGRTEKRR 367
           F DSF  DKKT LPK+V+WDG+++Y  VTG E+I    DH +++S+F+EGYREGRTEK++
Sbjct: 306 FVDSFHVDKKTNLPKIVVWDGDEEYDQVTGIETITQEEDHLTQVSRFLEGYREGRTEKKK 365

Query: 368 VAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTK-DDSEYNRVDTSARDQVEQTSSS 427
           + GP+ +GFINS+IGIR++YI+V +VA IM+ ++L + ++    R  T+ R++V+Q  ++
Sbjct: 366 INGPSFMGFINSMIGIRSVYILVFLVAVIMMLRSLGQVEEPTGVRTATAVRERVDQ--AT 425

Query: 428 SAAEVERSEYKAGDKED 442
           +  E E SE+K  DK++
Sbjct: 426 TVPEDESSEHKPSDKKE 439

BLAST of Chy3G050400 vs. ExPASy Swiss-Prot
Match: Q0JD42 (Protein disulfide isomerase-like 5-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDIL5-2 PE=2 SV=2)

HSP 1 Score: 474.2 bits (1219), Expect = 1.6e-132
Identity = 225/403 (55.83%), Postives = 310/403 (76.92%), Query Frame = 0

Query: 10  LFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYAPWCGHCKRL 69
           L LL +S   + + +  E P   DG+V++LD+S+F+ A+ + DY+ VDFYAPWCGHCKRL
Sbjct: 20  LLLLLLSFHAAAAAAAEEFP--RDGRVIELDESSFEAALGAIDYLFVDFYAPWCGHCKRL 79

Query: 70  SPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVPVDYYGPRKA 129
           +PELD AAP LA L EPI++AKVNADKY  L  KY VD +PT+ +F+HGVP++Y G RKA
Sbjct: 80  APELDEAAPVLAGLSEPIIVAKVNADKYRKLGSKYGVDGFPTLMLFIHGVPIEYTGSRKA 139

Query: 130 ELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKFGIKYKKKAW 189
           +LL R L KFVAPDVS+LESDS+I  FVE AG  FP+++GFG++ES+I+ +G KYKK+AW
Sbjct: 140 DLLVRNLNKFVAPDVSILESDSAIKSFVENAGTSFPMFIGFGVNESLIAGYGGKYKKRAW 199

Query: 190 FSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIKQSLFPLVLP 249
           F+VAK FSED MV+YDFDKVPALVS+HP Y  E+S+FYGPFE  FLE+FI+QSL PL +P
Sbjct: 200 FAVAKDFSEDFMVTYDFDKVPALVSLHPKY-KEQSVFYGPFEGSFLEDFIRQSLLPLTVP 259

Query: 250 INYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYVGAKQWGEFA 309
           IN +TLK+L DDDRK+ L I+ED+ ++ + +L+ +L++AA+ANRDLVF YVG KQW EF 
Sbjct: 260 INTETLKMLDDDDRKVVLAILEDDSDETSSQLVKVLRSAANANRDLVFGYVGIKQWDEFV 319

Query: 310 DSFG-DKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGRTEKRRVAG 369
           ++F   K + LPK+++WD  ++Y +V GSE +   D AS+IS+F+EGYR GRT K++V+G
Sbjct: 320 ETFDISKSSQLPKLIVWDRNEEYEVVEGSEKLEEGDQASQISQFLEGYRAGRTTKKKVSG 379

Query: 370 PAILGFINSLIGIRTIYIIVIIVA--GIMLYQNLTKDDSEYNR 410
           P+ +GF+NSL+ + ++YI++ + A  G+M+Y     D  +  R
Sbjct: 380 PSFMGFLNSLVSLNSLYILICVFALLGVMIYFTGQDDTPQVRR 419

BLAST of Chy3G050400 vs. ExPASy Swiss-Prot
Match: Q0E0I1 (Protein disulfide isomerase-like 5-3 OS=Oryza sativa subsp. japonica OX=39947 GN=PDIL5-3 PE=3 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 4.6e-119
Identity = 217/412 (52.67%), Postives = 294/412 (71.36%), Query Frame = 0

Query: 8   LVLFLLSISSLPSFSF--------STSELPLTADGKVLDLDDSNFDLAISSFDYILVDFY 67
           +V+ LL +  LP+ +            E  +  DG+VL+LDD NFD A+ +   + VDFY
Sbjct: 12  VVVLLLLVVVLPATTCGADAGGGGEAEEFQIPRDGRVLELDDGNFDAAVRAAGLLFVDFY 71

Query: 68  APWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGV 127
           APWCGHCKRL+P+LD AAP LA L  PIV+AKVNADKY  L  KY VD +PT+ +F HG 
Sbjct: 72  APWCGHCKRLAPQLDEAAPVLAGLSTPIVVAKVNADKYKKLGSKYGVDGFPTLMLFDHGT 131

Query: 128 PVDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISK 187
           P +Y G RKA+LL   LKK VAPDVSVLESDS+I  FVE AG  FP++LGFG+DES+I +
Sbjct: 132 PTEYTGSRKADLLVENLKKLVAPDVSVLESDSAIKSFVEDAGMGFPLFLGFGVDESLIVE 191

Query: 188 FGIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEE-QFLEEF 247
           +G KYK +AWFSVAK FSED+MV YDFDKVPALVS++P Y  E+SIFYGPF++  FLE+F
Sbjct: 192 YGAKYKNRAWFSVAKDFSEDMMVFYDFDKVPALVSVNPKY-REQSIFYGPFDDGAFLEDF 251

Query: 248 IKQSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQ-TKKLINLLKAAASANRDLVF 307
           I+ SL PLV+P+N +T+K+L DD RK+ L I++D++ D+ + +LI +L++AASANRDLVF
Sbjct: 252 IRNSLLPLVVPMNRETVKMLNDDGRKVVLMILQDDESDENSPRLIKVLRSAASANRDLVF 311

Query: 308 AYVGAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYR 367
            YVG  QW EF ++F  K + LP M++WD +++Y +V GSE +   D+ S+IS+F+EGYR
Sbjct: 312 GYVGVNQWEEFTETFDVKSSELPTMIVWDKKEEYEIVEGSERLEEGDYGSQISRFLEGYR 371

Query: 368 EGRTEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNR 410
            GRT K++V   +      +L+G+  +YI+V +VA ++L    +    E  R
Sbjct: 372 AGRTIKKKVGDRS-----PTLLGVNAVYILVFLVAVLVLLMYFSGQGEEDQR 417

BLAST of Chy3G050400 vs. ExPASy Swiss-Prot
Match: P54399 (Protein disulfide-isomerase OS=Drosophila melanogaster OX=7227 GN=Pdi PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.1e-27
Identity = 96/341 (28.15%), Postives = 164/341 (48.09%), Query Frame = 0

Query: 20  SFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQ 79
           S+  +++E  +  +  VL     NF   I+  +++LV+FYAPWCGHCK L+PE   AA Q
Sbjct: 13  SYVAASAEAEVKVEEGVLVATVDNFKQLIADNEFVLVEFYAPWCGHCKALAPEYAKAAQQ 72

Query: 80  LARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVPVDYYGPRKAELLARYLKKF 139
           LA  + PI LAKV+A     LA++Y V  YPT+K F  G PV+Y G R+A  +  ++ K 
Sbjct: 73  LAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPVEYSGGRQAADIIAWVTKK 132

Query: 140 VAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLD-ESVISKFGIKYKKKAWFSVAKGFSE 199
             P    L S +   +F++         +GF  D ES  +K   K    A  S   G S 
Sbjct: 133 TGPPAKDLTSVADAEQFLK---DNEIAIIGFFKDLESEEAKTFTKV-ANALDSFVFGVSS 192

Query: 200 DIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIKQSLFPLVLPINYDTLKLL 259
           +  V   ++     V +   +++++S+F G   E+ L++F +    PL++  N+++   +
Sbjct: 193 NADVIAKYEAKDNGVVLFKPFDDKKSVFEGELNEENLKKFAQVQSLPLIVDFNHESASKI 252

Query: 260 KDDDRKIALTIVEDEDEDQTKKLINLLKAAASANR-DLVFAYVGA--KQWGEFADSFGDK 319
                K  L      +    +K ++ LK  A   R D++F  + +  +      + FG  
Sbjct: 253 FGGSIKSHLLFFVSREGGHIEKYVDPLKEIAKKYRDDILFVTISSDEEDHTRIFEFFGMN 312

Query: 320 KTTLPKMVIWDGEDDYLMV-TGSESIVGNDHASEISKFIEG 356
           K  +P + +   E+D       S+ +      + + KF++G
Sbjct: 313 KEEVPTIRLIKLEEDMAKYKPESDDLSAETIEAFLKKFLDG 349

BLAST of Chy3G050400 vs. ExPASy Swiss-Prot
Match: P05307 (Protein disulfide-isomerase OS=Bos taurus OX=9913 GN=P4HB PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.2e-27
Identity = 99/333 (29.73%), Postives = 156/333 (46.85%), Query Frame = 0

Query: 36  VLDLDDSNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNAD 95
           VL L   NFD A+++  Y+LV+FYAPWCGHCK L+PE   AA +L      I LAKV+A 
Sbjct: 28  VLVLHKGNFDEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDAT 87

Query: 96  KYTSLAKKYDVDAYPTIKIFMHG---VPVDYYGPRKAELLARYLKKFVAPDVSVLESDSS 155
           + + LA++Y V  YPTIK F +G    P +Y   R+A+ +  +LKK   P  S L   ++
Sbjct: 88  EESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAASTLSDGAA 147

Query: 156 INEFVEAAGPYFPIYLGFGLDESVISKFGIKYKKKAWFSVAKGF--SEDIMVSYDFDKVP 215
               VE++       +GF  D    S        +    +  G   + D+   Y  DK  
Sbjct: 148 AEALVESSE---VAVIGFFKDMESDSAKQFFLAAEVIDDIPFGITSNSDVFSKYQLDKDG 207

Query: 216 ALVSIHPNYNNERSIFYGPFEEQFLEEFIKQSLFPLVLPINYDTL-KLLKDDDRKIALTI 275
             V +   ++  R+ F G   ++ L +FIK +  PLV+     T  K+   + +   L  
Sbjct: 208 --VVLFKKFDEGRNNFEGEVTKEKLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLF 267

Query: 276 VEDEDEDQTKKLINLLKAAASANRDLVFAYVGAKQWG--EFADSFGDKKTTLPKMVIWDG 335
           +     D   KL N  KAA S    ++F ++ +         + FG KK   P + +   
Sbjct: 268 LPKSVSDYEGKLSNFKKAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITL 327

Query: 336 EDDYLMVTGSESIVGNDHASEISKFIEGYREGR 361
           E++   +T  +       A +I++F   + EG+
Sbjct: 328 EEE---MTKYKPESDELTAEKITEFCHRFLEGK 352

BLAST of Chy3G050400 vs. ExPASy TrEMBL
Match: A0A0A0LQ17 (Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G403150 PE=4 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 2.8e-244
Identity = 437/441 (99.09%), Postives = 439/441 (99.55%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           MRRSSQFLVLFLLS+SSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA
Sbjct: 1   MRRSSQFLVLFLLSVSSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
           GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSE+IVGNDHASEISKFIEGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSETIVGNDHASEISKFIEGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEY RVDTSARDQVEQ
Sbjct: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYPRVDTSARDQVEQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 442
           TSSSSA EVERSEYKAGDKED
Sbjct: 421 TSSSSAPEVERSEYKAGDKED 441

BLAST of Chy3G050400 vs. ExPASy TrEMBL
Match: A0A5D3C662 (Protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001260 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.4e-240
Identity = 429/441 (97.28%), Postives = 436/441 (98.87%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           MRRS QFLVLFLLSIS+LPSFS S+SELPLTADGKVLDLD+SNFDLAISSFDYILVDFYA
Sbjct: 1   MRRSWQFLVLFLLSISALPSFSLSSSELPLTADGKVLDLDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
           GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPAL+SIHPNYNNERSIFYGPFEE FLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALISIHPNYNNERSIFYGPFEEPFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSF DKKTTLPKMVIWDGEDDYLMVTGSESI+GNDHASEISKFIEGYREGR
Sbjct: 301 GAKQWGEFADSFRDKKTTLPKMVIWDGEDDYLMVTGSESIIGNDHASEISKFIEGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TEKRRVAGPAILGFINSLIGIRTIYIIVIIVA IMLYQNLTK+DSEYNRVDTSARDQVEQ
Sbjct: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAVIMLYQNLTKEDSEYNRVDTSARDQVEQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 442
           TSSSSAAEV+RSEYKAGDKED
Sbjct: 421 TSSSSAAEVQRSEYKAGDKED 441

BLAST of Chy3G050400 vs. ExPASy TrEMBL
Match: A0A1S3B3L3 (protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485425 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 9.4e-240
Identity = 429/441 (97.28%), Postives = 436/441 (98.87%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           MRRS QFLVLFLLSIS+LPSFS S+SELPLTADGKVLDLD+SNFDLAISSFDYILVDFYA
Sbjct: 1   MRRSWQFLVLFLLSISALPSFSLSSSELPLTADGKVLDLDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
           GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPAL+SIHPNYNNERSIFYGPFEE FLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALISIHPNYNNERSIFYGPFEEPFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSF DKKTTLPKMVIWDGEDDYLMVTGSESI+GNDHASEISKFIEGYREGR
Sbjct: 301 GAKQWGEFADSFRDKKTTLPKMVIWDGEDDYLMVTGSESIIGNDHASEISKFIEGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TEKRRVAGPAILGFINSLIGIRTIYIIVIIVA IMLYQNLTK+DSEYNRVDTSARDQVEQ
Sbjct: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAVIMLYQNLTKEDSEYNRVDTSARDQVEQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 442
           TSSSSAAEV+RSEYKAGDKED
Sbjct: 421 TSSSSAAEVQRSEYKAGDKED 441

BLAST of Chy3G050400 vs. ExPASy TrEMBL
Match: A0A6J1H1U7 (protein disulfide-isomerase 5-2-like OS=Cucurbita moschata OX=3662 GN=LOC111459596 PE=4 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.5e-218
Identity = 379/441 (85.94%), Postives = 421/441 (95.46%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           M+RS Q +V+ L+SI +LPS SFS+SELPLTADG VL+LD+SNFDLAISSFDYIL+DFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILIDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLA+LKEPIVLAK+NADK+T LAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELL RYLKKFVAPDVS+LESDSSINEFVEA+GPYFPIYLGFGLDES+ISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
            IKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFE++FLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDD+RKIALTIV+DED+DQTK LINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKM+IWDG+++YLMV GSESI+GNDHASEISKF++GYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISKFVQGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TE+RRVAGP+ +GFINS+IG +++YIIV IVA IML+Q LTKDD+EY+RVDT ARDQV+Q
Sbjct: 361 TEERRVAGPSFIGFINSMIGTKSVYIIVFIVAVIMLFQYLTKDDNEYHRVDTPARDQVDQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 442
            S SS ++VER+EY+AGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of Chy3G050400 vs. ExPASy TrEMBL
Match: A0A6J1JQY3 (protein disulfide-isomerase 5-2-like OS=Cucurbita maxima OX=3661 GN=LOC111487041 PE=4 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 1.7e-217
Identity = 379/441 (85.94%), Postives = 420/441 (95.24%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           M+RS Q +V+ L+SI +LPS SFS+SELPLTADG VL+LD+SNFDLAISSFDYILVDFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLA+LKEPIVLAK+NADK+T LAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELL RYLKKFVAPDVS+LESDSSINEFVEA+GPYFPIYLGFGLDES+ISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
            IKYKKKAWFSVAKGFSEDIMVSYDFDKVP+LVSIHPNYNNERSIFYGPFE++FLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPSLVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDD+RKIALTIV+DED+DQTK LINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKM+IWDGE++YLMV GSESI+GNDHASEISKF+EGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGEEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TE+RRVAGP+ +GFINS+IG  ++YIIV IVA +ML+Q LTKDD+EY+RVDT A+DQV+Q
Sbjct: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPAQDQVDQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 442
            S SS ++VER+EY+AGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of Chy3G050400 vs. NCBI nr
Match: XP_004138646.1 (protein disulfide-isomerase 5-2 [Cucumis sativus] >KGN63109.1 hypothetical protein Csa_022564 [Cucumis sativus])

HSP 1 Score: 849 bits (2193), Expect = 4.60e-310
Identity = 437/441 (99.09%), Postives = 439/441 (99.55%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           MRRSSQFLVLFLLS+SSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA
Sbjct: 1   MRRSSQFLVLFLLSVSSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
           GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSE+IVGNDHASEISKFIEGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSETIVGNDHASEISKFIEGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEY RVDTSARDQVEQ
Sbjct: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYPRVDTSARDQVEQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 441
           TSSSSA EVERSEYKAGDKED
Sbjct: 421 TSSSSAPEVERSEYKAGDKED 441

BLAST of Chy3G050400 vs. NCBI nr
Match: XP_008441230.1 (PREDICTED: protein disulfide-isomerase 5-2 isoform X1 [Cucumis melo] >KAA0037003.1 protein disulfide-isomerase 5-2 isoform X1 [Cucumis melo var. makuwa] >TYK06648.1 protein disulfide-isomerase 5-2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 834 bits (2154), Expect = 4.04e-304
Identity = 429/441 (97.28%), Postives = 436/441 (98.87%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           MRRS QFLVLFLLSIS+LPSFS S+SELPLTADGKVLDLD+SNFDLAISSFDYILVDFYA
Sbjct: 1   MRRSWQFLVLFLLSISALPSFSLSSSELPLTADGKVLDLDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
           GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPAL+SIHPNYNNERSIFYGPFEE FLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALISIHPNYNNERSIFYGPFEEPFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSF DKKTTLPKMVIWDGEDDYLMVTGSESI+GNDHASEISKFIEGYREGR
Sbjct: 301 GAKQWGEFADSFRDKKTTLPKMVIWDGEDDYLMVTGSESIIGNDHASEISKFIEGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TEKRRVAGPAILGFINSLIGIRTIYIIVIIVA IMLYQNLTK+DSEYNRVDTSARDQVEQ
Sbjct: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAVIMLYQNLTKEDSEYNRVDTSARDQVEQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 441
           TSSSSAAEV+RSEYKAGDKED
Sbjct: 421 TSSSSAAEVQRSEYKAGDKED 441

BLAST of Chy3G050400 vs. NCBI nr
Match: XP_038886294.1 (protein disulfide-isomerase 5-2 [Benincasa hispida])

HSP 1 Score: 805 bits (2078), Expect = 1.48e-292
Identity = 409/441 (92.74%), Postives = 431/441 (97.73%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           M+RS Q +V+FLLSIS LPSFSFS++ELPLTADG VL+LD+SNFDLAISSFDYILVDFYA
Sbjct: 1   MQRSWQVVVVFLLSISELPSFSFSSAELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLA+LKEPIVLAKVNADK+TSLAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKVNADKFTSLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELLARYLKKFVAPDVS+LESDSSINEFVEAAGPYFPIYLGFGLDESVISKF
Sbjct: 121 VDYYGPRKAELLARYLKKFVAPDVSILESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
           GIKYKKKAWFSVAKGFSED+MV+YDFDKVP+LVSIHPNYNNERSIFYGPFEEQFLEEFIK
Sbjct: 181 GIKYKKKAWFSVAKGFSEDMMVTYDFDKVPSLVSIHPNYNNERSIFYGPFEEQFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDD+RKIALTIVEDED DQTK LINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVEDEDNDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESI+GNDHASEISKFIEGYREG+
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIIGNDHASEISKFIEGYREGK 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TEKRRVAGPAI+GFINSLIGIRT+YIIVIIVAGIMLYQNLTKDD+EY RVD  ARDQVEQ
Sbjct: 361 TEKRRVAGPAIIGFINSLIGIRTVYIIVIIVAGIMLYQNLTKDDNEYRRVDIPARDQVEQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 441
           TSSS++ +VERSEYKAGDKED
Sbjct: 421 TSSSTS-QVERSEYKAGDKED 440

BLAST of Chy3G050400 vs. NCBI nr
Match: XP_022958351.1 (protein disulfide-isomerase 5-2-like [Cucurbita moschata])

HSP 1 Score: 762 bits (1968), Expect = 8.82e-276
Identity = 379/441 (85.94%), Postives = 421/441 (95.46%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           M+RS Q +V+ L+SI +LPS SFS+SELPLTADG VL+LD+SNFDLAISSFDYIL+DFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSSSELPLTADGTVLELDESNFDLAISSFDYILIDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLA+LKEPIVLAK+NADK+T LAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELL RYLKKFVAPDVS+LESDSSINEFVEA+GPYFPIYLGFGLDES+ISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
            IKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFE++FLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDD+RKIALTIV+DED+DQTK LINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKM+IWDG+++YLMV GSESI+GNDHASEISKF++GYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISKFVQGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TE+RRVAGP+ +GFINS+IG +++YIIV IVA IML+Q LTKDD+EY+RVDT ARDQV+Q
Sbjct: 361 TEERRVAGPSFIGFINSMIGTKSVYIIVFIVAVIMLFQYLTKDDNEYHRVDTPARDQVDQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 441
            S SS ++VER+EY+AGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of Chy3G050400 vs. NCBI nr
Match: KAG7033399.1 (Protein disulfide-isomerase 5-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 760 bits (1963), Expect = 5.09e-275
Identity = 380/441 (86.17%), Postives = 419/441 (95.01%), Query Frame = 0

Query: 1   MRRSSQFLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYA 60
           M+RS Q +V+ L+SI +LPS SFS SELPLTADG VL+LD+SNFDLAISSFDYILVDFYA
Sbjct: 1   MQRSWQAVVIVLVSILALPSSSFSISELPLTADGTVLELDESNFDLAISSFDYILVDFYA 60

Query: 61  PWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVP 120
           PWCGHCKRLSPELDAAAPQLA+LKEPIVLAK+NADK+T LAKKYDVDAYPTIKIFMHGVP
Sbjct: 61  PWCGHCKRLSPELDAAAPQLAKLKEPIVLAKLNADKFTRLAKKYDVDAYPTIKIFMHGVP 120

Query: 121 VDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKF 180
           VDYYGPRKAELL RYLKKFVAPDVS+LESDSSINEFVEA+GPYFPIYLGFGLDES+ISKF
Sbjct: 121 VDYYGPRKAELLVRYLKKFVAPDVSILESDSSINEFVEASGPYFPIYLGFGLDESIISKF 180

Query: 181 GIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIK 240
            IKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFE++FLEEFIK
Sbjct: 181 AIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEDKFLEEFIK 240

Query: 241 QSLFPLVLPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV 300
           QSLFPLVLPINYDTLKLLKDD+RKIALTIV+DED+DQTK LINLLKAAASANRDLVFAYV
Sbjct: 241 QSLFPLVLPINYDTLKLLKDDERKIALTIVKDEDDDQTKNLINLLKAAASANRDLVFAYV 300

Query: 301 GAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDHASEISKFIEGYREGR 360
           GAKQWGEFADSFGDKKTTLPKM+IWDG+++YLMV GSESI+GNDHASEISKF+EGYREGR
Sbjct: 301 GAKQWGEFADSFGDKKTTLPKMIIWDGKEEYLMVIGSESIIGNDHASEISKFVEGYREGR 360

Query: 361 TEKRRVAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTKDDSEYNRVDTSARDQVEQ 420
           TE+RRVAGP+ +GFINS+IG  ++YIIV IVA +ML+Q LTKDD+EY+RVDT ARDQV+Q
Sbjct: 361 TEERRVAGPSFIGFINSMIGTWSVYIIVFIVAIVMLFQYLTKDDNEYHRVDTPARDQVDQ 420

Query: 421 TSSSSAAEVERSEYKAGDKED 441
            S SS ++VER+EY+AGDKED
Sbjct: 421 ISGSSTSQVERTEYRAGDKED 441

BLAST of Chy3G050400 vs. TAIR 10
Match: AT1G35620.1 (PDI-like 5-2 )

HSP 1 Score: 530.8 bits (1366), Expect = 1.1e-150
Identity = 267/437 (61.10%), Postives = 350/437 (80.09%), Query Frame = 0

Query: 8   LVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYAPWCGHCK 67
           L+L  +S  +L     ++S+   T DG VL+L DSNFD AIS+FD I VDFYAPWCGHCK
Sbjct: 6   LLLCWISFLTLSISISASSDDQFTLDGTVLELTDSNFDSAISTFDCIFVDFYAPWCGHCK 65

Query: 68  RLSPELDAAAPQLARLKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHGVPVDYYGPR 127
           RL+PELDAAAP LA+LK+PIV+AK+NADKY+ LA+K ++DA+PT+ ++ HGVP++YYGPR
Sbjct: 66  RLNPELDAAAPILAKLKQPIVIAKLNADKYSRLARKIEIDAFPTLMLYNHGVPMEYYGPR 125

Query: 128 KAELLARYLKKFVAPDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKFGIKYKKK 187
           KA+LL RYLKKFVAPDV+VLESDS++ EFVE AG +FP+++GFGL+ES+IS  G KYKKK
Sbjct: 126 KADLLVRYLKKFVAPDVAVLESDSTVKEFVEDAGTFFPVFIGFGLNESIISGLGRKYKKK 185

Query: 188 AWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEFIKQSLFPLV 247
           AWF+V+K  SED MVSYDFDK PALV+ HP Y NE S+FYGPFE+ FLEEF+KQS  PL+
Sbjct: 186 AWFAVSKEVSEDTMVSYDFDKAPALVANHPTY-NEHSVFYGPFEDGFLEEFVKQSFLPLI 245

Query: 248 LPINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYVGAKQWGE 307
           LPIN+DTLKLLKDD+RKI LTIVEDE  +  +KL   L+AAA ANRDLVF YVG KQ+ E
Sbjct: 246 LPINHDTLKLLKDDERKIVLTIVEDETHESLEKLYKALRAAAHANRDLVFGYVGVKQFEE 305

Query: 308 FADSFG-DKKTTLPKMVIWDGEDDYLMVTGSESIV-GNDHASEISKFIEGYREGRTEKRR 367
           F DSF  DKKT LPK+V+WDG+++Y  VTG E+I    DH +++S+F+EGYREGRTEK++
Sbjct: 306 FVDSFHVDKKTNLPKIVVWDGDEEYDQVTGIETITQEEDHLTQVSRFLEGYREGRTEKKK 365

Query: 368 VAGPAILGFINSLIGIRTIYIIVIIVAGIMLYQNLTK-DDSEYNRVDTSARDQVEQTSSS 427
           + GP+ +GFINS+IGIR++YI+V +VA IM+ ++L + ++    R  T+ R++V+Q  ++
Sbjct: 366 INGPSFMGFINSMIGIRSVYILVFLVAVIMMLRSLGQVEEPTGVRTATAVRERVDQ--AT 425

Query: 428 SAAEVERSEYKAGDKED 442
           +  E E SE+K  DK++
Sbjct: 426 TVPEDESSEHKPSDKKE 439

BLAST of Chy3G050400 vs. TAIR 10
Match: AT1G21750.1 (PDI-like 1-1 )

HSP 1 Score: 124.0 bits (310), Expect = 3.0e-28
Identity = 106/384 (27.60%), Postives = 180/384 (46.88%), Query Frame = 0

Query: 1   MRRSSQFLVLFL-LSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFY 60
           MR  + F +L L L  SS+ S    T E        VL LD +NF   I+  D+I+V+FY
Sbjct: 3   MRGFTLFSILVLSLCASSIRSEETETKEF-------VLTLDHTNFTDTINKHDFIVVEFY 62

Query: 61  APWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTS--LAKKYDVDAYPTIKIFMH 120
           APWCGHCK+L+PE + AA  L+    P+VLAK++A + T+   A +Y+V  +PTIKIF +
Sbjct: 63  APWCGHCKQLAPEYEKAASALSSNVPPVVLAKIDASEETNREFATQYEVQGFPTIKIFRN 122

Query: 121 G--VPVDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVE----AAGPYFPIYLGFG 180
           G     +Y GPR+AE +  YLKK   P  + ++S    +E V          FP   G  
Sbjct: 123 GGKAVQEYNGPREAEGIVTYLKKQSGPASAEIKSADDASEVVSDKKVVVVGIFPKLSGSE 182

Query: 181 LDESVISKFGIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFE 240
            D    S   I  K ++    A      ++   +      +V +   + +E+ +    F+
Sbjct: 183 FD----SFMAIAEKLRSELDFAHTSDAKLLPRGESSVTGPVVRLFKPF-DEQFVDSKDFD 242

Query: 241 EQFLEEFIKQSLFPLVLPINYD------TLKLLKDDDRKIALTIVEDEDEDQTKKLINLL 300
            + LE+F+K+S  PL+   + D       +K  +  + K  L I    +  ++ K     
Sbjct: 243 GEALEKFVKESSIPLITVFDKDPNNHPYVIKFFESTNTKAMLFINFTGEGAESLKSKYRE 302

Query: 301 KAAASANRDLVFAYVGAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDH 360
            A ++  + L F    A+        FG +++ +P ++I   +D   + T       N  
Sbjct: 303 VATSNKGQGLSFLLGDAENSQGAFQYFGLEESQVPLIIIQTADDKKYLKT-------NVE 362

Query: 361 ASEISKFIEGYREGRTEKRRVAGP 370
             +I  +++ +++G+    + + P
Sbjct: 363 VDQIESWVKDFKDGKIAPHKKSQP 367

BLAST of Chy3G050400 vs. TAIR 10
Match: AT1G21750.2 (PDI-like 1-1 )

HSP 1 Score: 124.0 bits (310), Expect = 3.0e-28
Identity = 106/384 (27.60%), Postives = 180/384 (46.88%), Query Frame = 0

Query: 1   MRRSSQFLVLFL-LSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFY 60
           MR  + F +L L L  SS+ S    T E        VL LD +NF   I+  D+I+V+FY
Sbjct: 3   MRGFTLFSILVLSLCASSIRSEETETKEF-------VLTLDHTNFTDTINKHDFIVVEFY 62

Query: 61  APWCGHCKRLSPELDAAAPQLARLKEPIVLAKVNADKYTS--LAKKYDVDAYPTIKIFMH 120
           APWCGHCK+L+PE + AA  L+    P+VLAK++A + T+   A +Y+V  +PTIKIF +
Sbjct: 63  APWCGHCKQLAPEYEKAASALSSNVPPVVLAKIDASEETNREFATQYEVQGFPTIKIFRN 122

Query: 121 G--VPVDYYGPRKAELLARYLKKFVAPDVSVLESDSSINEFVE----AAGPYFPIYLGFG 180
           G     +Y GPR+AE +  YLKK   P  + ++S    +E V          FP   G  
Sbjct: 123 GGKAVQEYNGPREAEGIVTYLKKQSGPASAEIKSADDASEVVSDKKVVVVGIFPKLSGSE 182

Query: 181 LDESVISKFGIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFE 240
            D    S   I  K ++    A      ++   +      +V +   + +E+ +    F+
Sbjct: 183 FD----SFMAIAEKLRSELDFAHTSDAKLLPRGESSVTGPVVRLFKPF-DEQFVDSKDFD 242

Query: 241 EQFLEEFIKQSLFPLVLPINYD------TLKLLKDDDRKIALTIVEDEDEDQTKKLINLL 300
            + LE+F+K+S  PL+   + D       +K  +  + K  L I    +  ++ K     
Sbjct: 243 GEALEKFVKESSIPLITVFDKDPNNHPYVIKFFESTNTKAMLFINFTGEGAESLKSKYRE 302

Query: 301 KAAASANRDLVFAYVGAKQWGEFADSFGDKKTTLPKMVIWDGEDDYLMVTGSESIVGNDH 360
            A ++  + L F    A+        FG +++ +P ++I   +D   + T       N  
Sbjct: 303 VATSNKGQGLSFLLGDAENSQGAFQYFGLEESQVPLIIIQTADDKKYLKT-------NVE 362

Query: 361 ASEISKFIEGYREGRTEKRRVAGP 370
             +I  +++ +++G+    + + P
Sbjct: 363 VDQIESWVKDFKDGKIAPHKKSQP 367

BLAST of Chy3G050400 vs. TAIR 10
Match: AT1G77510.1 (PDI-like 1-2 )

HSP 1 Score: 115.9 bits (289), Expect = 8.1e-26
Identity = 108/379 (28.50%), Postives = 178/379 (46.97%), Query Frame = 0

Query: 7   FLVLFLLSISSLPSFSFSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYAPWCGHC 66
           F +L LLS+      S  T E        VL LD SNF   IS  D+I+V+FYAPWCGHC
Sbjct: 9   FSILLLLSLFVSSIRSEETKEF-------VLTLDHSNFTETISKHDFIVVEFYAPWCGHC 68

Query: 67  KRLSPELDAAAPQLARLKEPIVLAKVNADKYTS--LAKKYDVDAYPTIKIFMHG--VPVD 126
           ++L+PE + AA +L+    P+ LAK++A +  +   A +Y +  +PT+KI  +G     D
Sbjct: 69  QKLAPEYEKAASELSSHNPPLALAKIDASEEANKEFANEYKIQGFPTLKILRNGGKSVQD 128

Query: 127 YYGPRKAELLARYLKKFVAP-DVSVLESDSS---INEFVEAAGPYFPIYLGFGLDESVIS 186
           Y GPR+AE +  YLKK   P  V +  +DS+   + E    A   FP   G   D    S
Sbjct: 129 YNGPREAEGIVTYLKKQSGPASVEIKSADSATEVVGEKNVVAVGVFPKLSGDEFD----S 188

Query: 187 KFGIKYKKKAWFSVAKGFSEDIMVSYDFDKVPALVSIHPNYNNERSIFYGPFEEQFLEEF 246
              +  K +A +  A       +   +  + PA+    P   +E  +    F  + LE+F
Sbjct: 189 FMALAEKLRADYDFAHTLDAKFLPRGESVEGPAVRLFKP--FDELFVDSKDFNGEALEKF 248

Query: 247 IKQSLFPLVL-----PINYDTLKLLKDDDRKIALTIVEDEDEDQTKKLINLLKAAASANR 306
           +K+S  PLV      P N+  +    +     A+  V        + L +  +  A++N+
Sbjct: 249 VKESSIPLVTVFDSDPNNHPYVAKFFESPATKAMMFVNFTGA-TAEALKSKYREVATSNK 308

Query: 307 DLVFAY-VGAKQWGEFA-DSFGDKKTTLPKMVIWDGED-DYLMVTGSESIVGNDHASEIS 366
           D   A+ VG  +  + A   FG +++ +P ++I   ++  YL V        N    +I 
Sbjct: 309 DQSLAFLVGDAESSQGAFQYFGLEESQVPLIIIQTPDNKKYLKV--------NVEVDQIE 365

Query: 367 KFIEGYREGRTEKRRVAGP 370
            + + +++G+    + + P
Sbjct: 369 SWFKDFQDGKVAVHKKSQP 365

BLAST of Chy3G050400 vs. TAIR 10
Match: AT3G54960.1 (PDI-like 1-3 )

HSP 1 Score: 103.2 bits (256), Expect = 5.4e-22
Identity = 85/314 (27.07%), Postives = 143/314 (45.54%), Query Frame = 0

Query: 23  FSTSELPLTADGKVLDLDDSNFDLAISSFDYILVDFYAPWCGHCKRLSPELDAAAPQLAR 82
           +    LP   +  V  L   NF   + +  + +V+FYAPWCG C+ L+PE  AAA +L  
Sbjct: 88  YEEEPLPPVDEKDVAVLTKDNFTEFVGNNSFAMVEFYAPWCGACQALTPEYAAAATELKG 147

Query: 83  LKEPIVLAKVNADKYTSLAKKYDVDAYPTIKIFMHG-VPVDYYGPRKAELLARYLKKFVA 142
           L     LAK++A +   LA+KY++  +PT+ +F+ G +   Y G R  + +  +LKK  +
Sbjct: 148 L---AALAKIDATEEGDLAQKYEIQGFPTVFLFVDGEMRKTYEGERTKDGIVTWLKKKAS 207

Query: 143 PDVSVLESDSSINEFVEAAGPYFPIYLGFGLDESVISKFGIKYKKKAWFSVAKGFSEDIM 202
           P +  + +     E V +A P         L  S   +     + +   S  +  S DI 
Sbjct: 208 PSIHNITTKEEA-ERVLSAEPKLVFGFLNSLVGSESEELAAASRLEDDLSFYQTASPDIA 267

Query: 203 VSYDFD---KVPALVSIHPNYNNERSIFYGPFEEQFLEEFIKQSLFPLVLPINYDTLKLL 262
             ++ +   K PALV +      + + F G F +  + EF+  +  PLV+    +   L+
Sbjct: 268 KLFEIETQVKRPALVLLKKE-EEKLARFDGNFTKTAIAEFVSANKVPLVINFTREGASLI 327

Query: 263 KDDDRKIALTIVEDEDEDQTKKLINLLKAAASANRDLVFAYV--GAKQWGEFADSFGDKK 322
            +   K  L +    +E + K L  L + A S     VF YV    + +GE    F    
Sbjct: 328 FESSVKNQLILFAKANESE-KHLPTLREVAKSFKGKFVFVYVQMDNEDYGEAVSGFFGVT 387

Query: 323 TTLPKMVIWDGEDD 331
              PK++++ G +D
Sbjct: 388 GAAPKVLVYTGNED 395

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94F091.5e-14961.10Protein disulfide-isomerase 5-2 OS=Arabidopsis thaliana OX=3702 GN=PDIL5-2 PE=1 ... [more]
Q0JD421.6e-13255.83Protein disulfide isomerase-like 5-2 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q0E0I14.6e-11952.67Protein disulfide isomerase-like 5-3 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P543991.1e-2728.15Protein disulfide-isomerase OS=Drosophila melanogaster OX=7227 GN=Pdi PE=2 SV=1[more]
P053074.2e-2729.73Protein disulfide-isomerase OS=Bos taurus OX=9913 GN=P4HB PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LQ172.8e-24499.09Thioredoxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G403150... [more]
A0A5D3C6629.4e-24097.28Protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B3L39.4e-24097.28protein disulfide-isomerase 5-2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034854... [more]
A0A6J1H1U73.5e-21885.94protein disulfide-isomerase 5-2-like OS=Cucurbita moschata OX=3662 GN=LOC1114595... [more]
A0A6J1JQY31.7e-21785.94protein disulfide-isomerase 5-2-like OS=Cucurbita maxima OX=3661 GN=LOC111487041... [more]
Match NameE-valueIdentityDescription
XP_004138646.14.60e-31099.09protein disulfide-isomerase 5-2 [Cucumis sativus] >KGN63109.1 hypothetical prote... [more]
XP_008441230.14.04e-30497.28PREDICTED: protein disulfide-isomerase 5-2 isoform X1 [Cucumis melo] >KAA0037003... [more]
XP_038886294.11.48e-29292.74protein disulfide-isomerase 5-2 [Benincasa hispida][more]
XP_022958351.18.82e-27685.94protein disulfide-isomerase 5-2-like [Cucurbita moschata][more]
KAG7033399.15.09e-27586.17Protein disulfide-isomerase 5-2, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
Match NameE-valueIdentityDescription
AT1G35620.11.1e-15061.10PDI-like 5-2 [more]
AT1G21750.13.0e-2827.60PDI-like 1-1 [more]
AT1G21750.23.0e-2827.60PDI-like 1-1 [more]
AT1G77510.18.1e-2628.50PDI-like 1-2 [more]
AT3G54960.15.4e-2227.07PDI-like 1-3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00421THIOREDOXINcoord: 54..62
score: 63.02
coord: 105..116
score: 37.27
coord: 62..71
score: 60.33
NoneNo IPR availablePFAMPF13848Thioredoxin_6coord: 190..332
e-value: 2.2E-13
score: 50.4
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 232..365
e-value: 4.7E-14
score: 54.8
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 24..152
e-value: 4.3E-35
score: 122.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 414..429
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 411..441
NoneNo IPR availablePANTHERPTHR18929:SF218PROTEIN DISULFIDE-ISOMERASE 5-2coord: 10..441
NoneNo IPR availablePANTHERPTHR18929PROTEIN DISULFIDE ISOMERASEcoord: 10..441
NoneNo IPR availableCDDcd02961PDI_a_familycoord: 37..136
e-value: 3.6846E-33
score: 118.482
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 36..137
e-value: 2.6E-25
score: 88.4
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 12..141
score: 15.314689
IPR017937Thioredoxin, conserved sitePROSITEPS00194THIOREDOXIN_1coord: 55..73
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 28..139
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 243..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G050400.1Chy3G050400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0034976 response to endoplasmic reticulum stress
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003756 protein disulfide isomerase activity