Chy3G050360 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G050360
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Subtilisin-like protease SBT3.5 isoform X1
Location	chrH03: 586418 .. 588529 (+)
RNA-Seq Expression	Chy3G050360
Synteny	Chy3G050360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTTCTGATGAAGCTTCTTCAACTCATTCCATAGCTTCATCCATACCCTCTTCACGCCCCGCAGTTCACATTGTGTACACCGAAACGCCTCCGGATGAGGACCCCAAGCACTTCCATATCCGAACCCTGGCTTCTGCTCTCGGAAGGTTCGTCTCTTTACTTCTCTCTGCTTTCCGGTTCACGTTTTTCGAAATGCAAAAGAAGATGATCGGACCTATGCTTCTTTTTTTGCATTTCCAGTGAAGAGGCTGCCAAGGATGCGCTTGTTTACAGCTACAAAACGGCTGTTAGTGGATTCTCTGCAAAGCTCACTCCCGACCAGGTTTCTCGAGTTTCCCGTAAGTTTAGCTTCAGTGGTTGCGTTTTCGAGTATATAATTAAGTTATAATCATTATGAGACTGCTGCTACTGATTCTTTAGTTTGCGAAATCCATTGTTGTAATTGGGTTGATTGAATTTGATCAAGGATGATGTCTAGGGGTTCATCATCTTCATAACTCTGATCAGATGATGAGTTAAGCTTTGAGTAGTTTTCATCTTCTGCATTCATCGTTTTATCTTTGTTTGTGCGTTGCGTAGCAAAGGGTATCCTAATTACATCCTATATTTTGGATTTAGGTTCCCAAAATGCACACCCATATCTAACACATTGAGTTTACGCGTCTCATTTCCATGCGTTTGGGTTTCCATACTGCGCTTCATCTTCTCATGCCGCTTCTATGAACTCCTTCCGCCACCTGCTTCAGTGAAACCCCTCTCTTTTCCCTCTTTCTATCGCCATGGATGAAGCTCTATCTTCAATTTCTACTCTCTCCATTTGCTTTGAGGAATTGCTCTCTCTCTCTCTCTCTCTCATTTGCGCTTCCATGATCTCCTCTGCTAATCAGATTCTTGACTCGAGAGGAAGTTTCTGGGGTGGGGGAGATAGAAGCTTGACAGAGTGAGAGAGAGTTGGAAATTGGTAAAATGGAGGAATGAGTAGGGAAAAGAGAAGAGAAGAAATAGTCGGGTGCTCTGTTTTGACGATATGGTTCTTTCTGCCTCATCTTTGGCATTCGATTTTCCATTTTTGTCAGGTTGGTCGACCTTTTTCCCAAATTCTAATTGTTTATTCCTCTGATTATTCATCCGTTAATCTCGTGGTGGAACTTGTTCATGTAGTTTGCTTGTTTTCTGTAGCACTAATTGTTTGGTTTAGTTAACCGAACAACCTTGGTTTTGGTTTCGTTCCTTTGTTGATTTGAATTTTGTTTAATGCGTTGGTGTTTTGTTCCAGTTTTTTCATTTTAGAAATCATAGTGTGCTACTGTTTTTCTCCGGACTCGTTTGTGCTTTTAAATCTTCCTCAATCAAATTGAAGAATCTAATTTATTATCAATAATTATATTATTAACTACACCCATCTTCCTCACTAACCACCTTTTTTTCTTTATAATTGCTGTTCTGGATGAACCCTACAACTCTCCTCACCCCTCCCATTAACCATTACCGCTCTCTGCGTCGCCTTCCTTTGCTTCATCTTAGACCCACCAGCATTGCATGGTATAGGCTGGGACAAGAAATCAAATGCCGTAGTAACATCCTTTAGAGGTAGGAAGAGAAAACGAGCTGATATTATTGAAATCTTAAGAGATTTTTCTACATTTTTCTAAAATGCTAAACGTTCTGCTTCTAAAGTTGGTTGTACCCTGGGTGAGGGGTGAAGAAGAGGAGGAAACGACCAATAGACACTTGGTGGTTGACTAATTGCGTCAGATACAGGGTCGCATCAATGATCATCTTACCAGGGCGATAGGAATATCATTTGGAGGAAGGAAGAGGAAACGGGTCGATTTAACTAACATAATTGGGAAGTTTCAGTTTGAGATGAGAATGCATAACTTAGAGCTTCAAAGGATTGTGTACTTGATTACAGGTGAGAAAGAGGAGGAGAAGGCCAAATGGTTGGTTCATGAACTTTATGGGTTACCGGGTTTGAGCTCTGACGATGCTCTTGAGATTTTTCTAATGTTGTTTGATGATGTCCAAAAGCTCGATTGCTTTCTTAGTTTACATATGAACAAGAAATCTGAGTATTGCATGGATCTTCTTGGCTGGCGCAGCTGA

mRNA sequence

ATGGCTGCTTCTGATGAAGCTTCTTCAACTCATTCCATAGCTTCATCCATACCCTCTTCACGCCCCGCAGTTCACATTGTGTACACCGAAACGCCTCCGGATGAGGACCCCAAGCACTTCCATATCCGAACCCTGGCTTCTGCTCTCGGAAGTGAAGAGGCTGCCAAGGATGCGCTTGTTTACAGCTACAAAACGGCTGTTAGTGGATTCTCTGCAAAGCTCACTCCCGACCAGATACAGGGTCGCATCAATGATCATCTTACCAGGGCGATAGGAATATCATTTGGAGGAAGGAAGAGGAAACGGGTCGATTTAACTAACATAATTGGGAAGTTTCAGTTTGAGATGAGAATGCATAACTTAGAGCTTCAAAGGATTGTGTACTTGATTACAGGTGAGAAAGAGGAGGAGAAGGCCAAATGGTTGGTTCATGAACTTTATGGGTTACCGGGTTTGAGCTCTGACGATGCTCTTGAGATTTTTCTAATGTTGTTTGATGATGTCCAAAAGCTCGATTGCTTTCTTAGTTTACATATGAACAAGAAATCTGAGTATTGCATGGATCTTCTTGGCTGGCGCAGCTGA

Coding sequence (CDS)

ATGGCTGCTTCTGATGAAGCTTCTTCAACTCATTCCATAGCTTCATCCATACCCTCTTCACGCCCCGCAGTTCACATTGTGTACACCGAAACGCCTCCGGATGAGGACCCCAAGCACTTCCATATCCGAACCCTGGCTTCTGCTCTCGGAAGTGAAGAGGCTGCCAAGGATGCGCTTGTTTACAGCTACAAAACGGCTGTTAGTGGATTCTCTGCAAAGCTCACTCCCGACCAGATACAGGGTCGCATCAATGATCATCTTACCAGGGCGATAGGAATATCATTTGGAGGAAGGAAGAGGAAACGGGTCGATTTAACTAACATAATTGGGAAGTTTCAGTTTGAGATGAGAATGCATAACTTAGAGCTTCAAAGGATTGTGTACTTGATTACAGGTGAGAAAGAGGAGGAGAAGGCCAAATGGTTGGTTCATGAACTTTATGGGTTACCGGGTTTGAGCTCTGACGATGCTCTTGAGATTTTTCTAATGTTGTTTGATGATGTCCAAAAGCTCGATTGCTTTCTTAGTTTACATATGAACAAGAAATCTGAGTATTGCATGGATCTTCTTGGCTGGCGCAGCTGA

Protein sequence

MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGISFGGRKRKRVDLTNIIGKFQFEMRMHNLELQRIVYLITGEKEEEKAKWLVHELYGLPGLSSDDALEIFLMLFDDVQKLDCFLSLHMNKKSEYCMDLLGWRS*

Homology

[Close]

BLAST of Chy3G050360 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 5.2e-06
Identity = 30/63 (47.62%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 19 SSRPAVHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLT 78
          S++  VHIVY      +DP+     H R L S LGS+E A D++VYSY+   SGF+AKLT
Sbjct: 29 SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 88

[Close]

BLAST of Chy3G050360 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-05
Identity = 28/63 (44.44%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 19 SSRPAVHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLT 78
          S++  VHIVY      +DPK     H + L+S LGS++ A +++VYSY+   SGF+AKLT
Sbjct: 24 SAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLT 83

[Close]

BLAST of Chy3G050360 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 2.6e-05
Identity = 28/58 (48.28%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 24 VHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLTPDQ 79
          VHIVY       DP+     H + LAS LGS++ A D++VYSY+   SGF+AKLT  Q
Sbjct: 32 VHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQ 89

[Close]

BLAST of Chy3G050360 vs. ExPASy Swiss-Prot
Match: Q9C7U8 (Subtilisin-like protease SBT3.17 OS=Arabidopsis thaliana OX=3702 GN=SBT3.17 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-04
Identity = 26/58 (44.83%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 24 VHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLTPDQ 79
          +HIV+      + P+     H + L   LGS+EAAK++LVY+YK   SGF+AKLT  Q
Sbjct: 37 IHIVHLGAKQHDTPELVTKSHYQILEPLLGSKEAAKNSLVYNYKHGFSGFAAKLTASQ 94

[Close]

BLAST of Chy3G050360 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 2.2e-04
Identity = 27/63 (42.86%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 19 SSRPAVHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLT 78
          S    VHIVY      +DP+     H + L+S LGS+  A +++VYSY+   SGF+AKLT
Sbjct: 24 SDESKVHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLT 83

[Close]

BLAST of Chy3G050360 vs. ExPASy TrEMBL
Match: A0A5A7T617 (Subtilisin-like protease SBT3.5 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001220 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 3.0e-57
Identity = 136/198 (68.69%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA S EASSTHSIASS+PSS PAVHIVYTETPPDE+PK FHIRTLASALGSEEAAKDALV
Sbjct: 1   MADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHIRTLASALGSEEAAKDALV 60

Query: 61  YSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGIS---FGGRKRKRVDLTNII-GKFQFEM 120
           YSYKTAV GFSAKLTPDQ+         R   I         R RV+   ++ G++++  
Sbjct: 61  YSYKTAVGGFSAKLTPDQVSRVSRVKKRRKSPIDNWWLTNCVRCRVESMIVLPGRYEYP- 120

Query: 121 RMHNLELQRIVYLITGEKEEEKAKWLVHELYGLPGLSSDDALEIFLMLFDDVQKLDCFLS 180
               LE  R     + EKEEEKAKWLV ELYGLPGLSS DALEIFLMLFD+VQK DCFLS
Sbjct: 121 ----LEEGR----GSSEKEEEKAKWLVDELYGLPGLSSVDALEIFLMLFDNVQKTDCFLS 180

Query: 181 LHMNKKSEYCMDLLGWRS 195
           LHMNKK EYCMDLLG RS
Sbjct: 181 LHMNKKYEYCMDLLGRRS 189

[Close]

BLAST of Chy3G050360 vs. ExPASy TrEMBL
Match: A0A0A0LMH0 (Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402130 PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 1.3e-36
Identity = 105/195 (53.85%), Postives = 112/195 (57.44%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA SDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV
Sbjct: 25  MADSDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 84

Query: 61  YSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGISFGGRKRKRVDLTNIIGKFQFEMRMHN 120
           YSYKTAVSGFSAKLTPDQ+                                         
Sbjct: 85  YSYKTAVSGFSAKLTPDQVS---------------------------------------- 144

Query: 121 LELQRIVYLITGEKEEEKAKWLVHELYGLPGLSSDDALEIFLMLFDDVQKLDCFLSLH-- 180
                    ++ EKEEEK K LV ELYGLPG ++D AL               FLSLH  
Sbjct: 145 --------RVSREKEEEKDKSLVDELYGLPG-AADHALR--------------FLSLHTL 156

Query: 181 -MNKKSEYCMDLLGW 193
              ++S  C    GW
Sbjct: 205 VTTRRSTGCSRQPGW 156

[Close]

BLAST of Chy3G050360 vs. ExPASy TrEMBL
Match: A0A5A7T2J7 (Subtilisin-like protease SBT3.17 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001210 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 4.3e-35
Identity = 93/192 (48.44%), Postives = 120/192 (62.50%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA SDEASSTHS+ASSI SSRP  HI YTETPPDEDPKHFHIRTLASALGSEEA K+ALV
Sbjct: 1   MADSDEASSTHSLASSISSSRPEGHIAYTETPPDEDPKHFHIRTLASALGSEEAPKNALV 60

Query: 61  YSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGISFGGRKRKRVDLTNIIGKFQFEMRMHN 120
           YSYKTAV GFSAKLTPDQ+  R++   +     S    +++      I+   +  M   N
Sbjct: 61  YSYKTAVGGFSAKLTPDQV-SRVSRRSSEGRNASSRSMRKRGGQTAEIVEVIRNTMEFMN 120

Query: 121 LELQRIVYLITGEK--EEEKAKWLVHELYGLPGLSSDDALEIFLMLFDDVQKLDCFLSLH 180
            +L+ I      ++  E+E    +V +L  +P LSS   +++  ++    Q ++ FL + 
Sbjct: 121 NQLKTIAERPKKQRQAEDEYRAEVVKQLQDIPELSSRGKVQLMRIIMHSFQDMEAFLMIP 180

Query: 181 MNKKSEYCMDLL 191
              K EYC  LL
Sbjct: 181 TELKLEYCTVLL 191

[Close]

BLAST of Chy3G050360 vs. ExPASy TrEMBL
Match: A0A1S3B3P2 (subtilisin-like protease SBT3.17 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485420 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 1.3e-28
Identity = 76/126 (60.32%), Postives = 89/126 (70.63%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA SDEASSTHS+ASSI SSRP  HI YTETPPDEDPKHFHIRTLASALGSEEA K+ALV
Sbjct: 1   MADSDEASSTHSLASSISSSRPEGHIAYTETPPDEDPKHFHIRTLASALGSEEAPKNALV 60

Query: 61  YSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGISFGGRKRKRVDLTNIIGKFQFEMRMHN 120
           YSYKTAV GFSAKLTPDQ+  R++   +     S    +++      I+   +  M   N
Sbjct: 61  YSYKTAVGGFSAKLTPDQV-SRVSRRSSEGRNASSRSMRKRGGQTAEIVEVIRNTMEFMN 120

Query: 121 LELQRI 127
            +L+ I
Sbjct: 121 NQLKTI 125

[Close]

BLAST of Chy3G050360 vs. ExPASy TrEMBL
Match: A0A1S3B2X4 (subtilisin-like protease SBT3.6 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103485421 PE=4 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.3e-28
Identity = 71/79 (89.87%), Postives = 74/79 (93.67%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA S EASSTHSIASS+PSS PAVHIVYTETPPDE+PK FHIRTLASALGSEEAAKDALV
Sbjct: 25  MADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHIRTLASALGSEEAAKDALV 84

Query: 61  YSYKTAVSGFSAKLTPDQI 80
           YSYKTAV GFSAKLTPDQ+
Sbjct: 85  YSYKTAVGGFSAKLTPDQV 103

[Close]

BLAST of Chy3G050360 vs. NCBI nr
Match: XP_004138648.1 (subtilisin-like protease SBT3.5 isoform X1 [Cucumis sativus])

HSP 1 Score: 228 bits (581), Expect = 4.69e-73
Identity = 130/194 (67.01%), Postives = 135/194 (69.59%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA SDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV
Sbjct: 25  MADSDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 84

Query: 61  YSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGISFGGRKRKRVDLTNIIGKFQFEMRMHN 120
           YSYKTAVSGFSAKLTPDQ+                                         
Sbjct: 85  YSYKTAVSGFSAKLTPDQVSR--------------------------------------- 144

Query: 121 LELQRIVYLITGEKEEEKAKWLVHELYGLPGLSSDDALEIFLMLFDDVQKLDCFLSLHMN 180
                    ++ EKEEEK K LV ELYGLPGLSS++ALEIFLMLFDDVQK DCFLS HMN
Sbjct: 145 ---------VSREKEEEKDKSLVDELYGLPGLSSNNALEIFLMLFDDVQKTDCFLSFHMN 170

Query: 181 KKSEYCMDLLGWRS 194
           KK EYCMDLLG RS
Sbjct: 205 KKYEYCMDLLGRRS 170

[Close]

BLAST of Chy3G050360 vs. NCBI nr
Match: KAA0037007.1 (subtilisin-like protease SBT3.5 isoform X1 [Cucumis melo var. makuwa] >TYK06644.1 subtilisin-like protease SBT3.5 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 228 bits (581), Expect = 8.80e-73
Identity = 136/198 (68.69%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA S EASSTHSIASS+PSS PAVHIVYTETPPDE+PK FHIRTLASALGSEEAAKDALV
Sbjct: 1   MADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHIRTLASALGSEEAAKDALV 60

Query: 61  YSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGIS---FGGRKRKRVDLTNII-GKFQFEM 120
           YSYKTAV GFSAKLTPDQ+         R   I         R RV+   ++ G++++  
Sbjct: 61  YSYKTAVGGFSAKLTPDQVSRVSRVKKRRKSPIDNWWLTNCVRCRVESMIVLPGRYEYP- 120

Query: 121 RMHNLELQRIVYLITGEKEEEKAKWLVHELYGLPGLSSDDALEIFLMLFDDVQKLDCFLS 180
               LE  R     + EKEEEKAKWLV ELYGLPGLSS DALEIFLMLFD+VQK DCFLS
Sbjct: 121 ----LEEGRG----SSEKEEEKAKWLVDELYGLPGLSSVDALEIFLMLFDNVQKTDCFLS 180

Query: 181 LHMNKKSEYCMDLLGWRS 194
           LHMNKK EYCMDLLG RS
Sbjct: 181 LHMNKKYEYCMDLLGRRS 189

[Close]

BLAST of Chy3G050360 vs. NCBI nr
Match: KAA0037008.1 (subtilisin-like protease SBT3.17 isoform X1 [Cucumis melo var. makuwa] >TYK06643.1 subtilisin-like protease SBT3.17 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 155 bits (392), Expect = 4.80e-44
Identity = 93/192 (48.44%), Postives = 120/192 (62.50%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA SDEASSTHS+ASSI SSRP  HI YTETPPDEDPKHFHIRTLASALGSEEA K+ALV
Sbjct: 1   MADSDEASSTHSLASSISSSRPEGHIAYTETPPDEDPKHFHIRTLASALGSEEAPKNALV 60

Query: 61  YSYKTAVSGFSAKLTPDQIQGRINDHLTRAIGISFGGRKRKRVDLTNIIGKFQFEMRMHN 120
           YSYKTAV GFSAKLTPDQ+  R++   +     S    +++      I+   +  M   N
Sbjct: 61  YSYKTAVGGFSAKLTPDQVS-RVSRRSSEGRNASSRSMRKRGGQTAEIVEVIRNTMEFMN 120

Query: 121 LELQRIVYLITGEK--EEEKAKWLVHELYGLPGLSSDDALEIFLMLFDDVQKLDCFLSLH 180
            +L+ I      ++  E+E    +V +L  +P LSS   +++  ++    Q ++ FL + 
Sbjct: 121 NQLKTIAERPKKQRQAEDEYRAEVVKQLQDIPELSSRGKVQLMRIIMHSFQDMEAFLMIP 180

Query: 181 MNKKSEYCMDLL 190
              K EYC  LL
Sbjct: 181 TELKLEYCTVLL 191

[Close]

BLAST of Chy3G050360 vs. NCBI nr
Match: XP_011649903.1 (subtilisin-like protease SBT3.6 isoform X2 [Cucumis sativus] >KAE8652245.1 hypothetical protein Csa_022143 [Cucumis sativus])

HSP 1 Score: 147 bits (372), Expect = 6.01e-42
Identity = 77/79 (97.47%), Postives = 78/79 (98.73%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA SDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV
Sbjct: 25  MADSDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 84

Query: 61  YSYKTAVSGFSAKLTPDQI 79
           YSYKTAVSGFSAKLTPDQ+
Sbjct: 85  YSYKTAVSGFSAKLTPDQV 103

[Close]

BLAST of Chy3G050360 vs. NCBI nr
Match: XP_008441225.1 (PREDICTED: subtilisin-like protease SBT3.6 isoform X4 [Cucumis melo])

HSP 1 Score: 133 bits (335), Expect = 1.43e-36
Identity = 71/79 (89.87%), Postives = 74/79 (93.67%), Query Frame = 0


Query: 1   MAASDEASSTHSIASSIPSSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALV 60
           MA S EASSTHSIASS+PSS PAVHIVYTETPPDE+PK FHIRTLASALGSEEAAKDALV
Sbjct: 25  MADSVEASSTHSIASSMPSSPPAVHIVYTETPPDENPKDFHIRTLASALGSEEAAKDALV 84

Query: 61  YSYKTAVSGFSAKLTPDQI 79
           YSYKTAV GFSAKLTPDQ+
Sbjct: 85  YSYKTAVGGFSAKLTPDQV 103

[Close]

BLAST of Chy3G050360 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 96.3 bits (238), Expect = 2.9e-20
Identity = 45/61 (73.77%), Postives = 53/61 (86.89%), Query Frame = 0


Query: 19  SSRPAVHIVYTETPPDEDPKHFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLTPDQ 78
           SS   VHI+YTE P DE+PK +H+RTL+SALGSEEAAKDAL+YSYK A SGFSAKLTP+Q
Sbjct: 44  SSEAKVHIIYTEKPTDEEPKTYHLRTLSSALGSEEAAKDALIYSYKEAASGFSAKLTPEQ 103

Query: 79  I 80
           +
Sbjct: 104 V 104

[Close]

BLAST of Chy3G050360 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.7e-07
Identity = 30/63 (47.62%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 19 SSRPAVHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLT 78
          S++  VHIVY      +DP+     H R L S LGS+E A D++VYSY+   SGF+AKLT
Sbjct: 29 SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 88

[Close]

BLAST of Chy3G050360 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.7e-07
Identity = 30/63 (47.62%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 19  SSRPAVHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLT 78
           S++  VHIVY      +DP+     H R L S LGS+E A D++VYSY+   SGF+AKLT
Sbjct: 45  SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLT 104

[Close]

BLAST of Chy3G050360 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 51.6 bits (122), Expect = 8.3e-07
Identity = 28/63 (44.44%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 19 SSRPAVHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLT 78
          S++  VHIVY      +DPK     H + L+S LGS++ A +++VYSY+   SGF+AKLT
Sbjct: 24 SAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLT 83

[Close]

BLAST of Chy3G050360 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.8e-06
Identity = 28/58 (48.28%), Postives = 36/58 (62.07%), Query Frame = 0


Query: 24 VHIVYTETPPDEDPK---HFHIRTLASALGSEEAAKDALVYSYKTAVSGFSAKLTPDQ 79
          VHIVY       DP+     H + LAS LGS++ A D++VYSY+   SGF+AKLT  Q
Sbjct: 32 VHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQ 89

The following BLAST results are available for this feature:

BLAST of Chy3G050360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7I2	5.2e-06	47.62	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
F4HPF1	1.2e-05	44.44	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]
Q9MAP5	2.6e-05	48.28	Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S...	[more]
Q9C7U8	1.3e-04	44.83	Subtilisin-like protease SBT3.17 OS=Arabidopsis thaliana OX=3702 GN=SBT3.17 PE=3...	[more]
Q9MAP7	2.2e-04	42.86	Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S...	[more]

BLAST of Chy3G050360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T617	3.0e-57	68.69	Subtilisin-like protease SBT3.5 isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A0A0LMH0	1.3e-36	53.85	Inhibitor I9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40213...	[more]
A0A5A7T2J7	4.3e-35	48.44	Subtilisin-like protease SBT3.17 isoform X1 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B3P2	1.3e-28	60.32	subtilisin-like protease SBT3.17 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485...	[more]
A0A1S3B2X4	2.3e-28	89.87	subtilisin-like protease SBT3.6 isoform X3 OS=Cucumis melo OX=3656 GN=LOC1034854...	[more]

BLAST of Chy3G050360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138648.1	4.69e-73	67.01	subtilisin-like protease SBT3.5 isoform X1 [Cucumis sativus]	[more]
KAA0037007.1	8.80e-73	68.69	subtilisin-like protease SBT3.5 isoform X1 [Cucumis melo var. makuwa] >TYK06644....	[more]
KAA0037008.1	4.80e-44	48.44	subtilisin-like protease SBT3.17 isoform X1 [Cucumis melo var. makuwa] >TYK06643...	[more]
XP_011649903.1	6.01e-42	97.47	subtilisin-like protease SBT3.6 isoform X2 [Cucumis sativus] >KAE8652245.1 hypot...	[more]
XP_008441225.1	1.43e-36	89.87	PREDICTED: subtilisin-like protease SBT3.6 isoform X4 [Cucumis melo]	[more]

BLAST of Chy3G050360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	2.9e-20	73.77	Proteinase inhibitor, propeptide	[more]
AT4G10550.1	3.7e-07	47.62	Subtilase family protein	[more]
AT4G10550.3	3.7e-07	47.62	Subtilase family protein	[more]
AT1G32950.1	8.3e-07	44.44	Subtilase family protein	[more]
AT1G32960.1	1.8e-06	48.28	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 38..80 e-value: 6.1E-6 score: 26.8
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 6..85 e-value: 7.7E-14 score: 53.9
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 11..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G050360.1	Chy3G050360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008233	peptidase activity