Homology
BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match:
F4IYF8 (Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760 PE=2 SV=1)
HSP 1 Score: 981.5 bits (2536), Expect = 6.3e-285
Identity = 536/888 (60.36%), Postives = 659/888 (74.21%), Query Frame = 0
Query: 6 EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHLL-IPD 65
E S ITLGGKGSSLSSSSVY +A G A VRIDSSA++R S+ N SIK IP
Sbjct: 4 ERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGIPQ 63
Query: 66 FLTREEARASLVVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTNE 125
LT EE RASL VLLNKLI+S SSS RSV+P+ I E LN K E+L+ +DVT
Sbjct: 64 GLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTEG 123
Query: 126 ELSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASK 185
E V ++SC L G+C+++DH+ST LS I D+VA +SCE +KAD+++FSL+DSGDG K
Sbjct: 124 ENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGDK 183
Query: 186 EEVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGK 245
+ +GVA D+KVLLNG K G++E E ISKIP +HG R K VH+ R ELNS VK GK
Sbjct: 184 DVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGGK 243
Query: 246 GGSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPS 305
GS ++G + T LL + +LG CS R KL DEN+K L+ + C
Sbjct: 244 TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLSEKI---CVE 303
Query: 306 NESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQD 365
NE+LK YKL L+E Y F H +N L VW+I EA AA + GGEL +
Sbjct: 304 NENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKSG 363
Query: 366 VSTNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGN-----GGGLGSLENLVKDLL 425
+ E ++ KK + +KKAV+GKGTS+V+Q IKDRL N G + SL + +L
Sbjct: 364 DADKEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQIL 423
Query: 426 SFLDPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFER 485
+ +P+ FD+LL K+K+IVESNE+RRLPKLPKGTRDFAKEQM +R+KAFSII++VF+R
Sbjct: 424 NLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVFKR 483
Query: 486 HGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTS 545
HGAT LDTP FELRETL GKYGEDSKL++D+ADQGGEL SLRYDLTVPFARYVAMNG+TS
Sbjct: 484 HGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGITS 543
Query: 546 FKRYQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYE 605
FKRYQIAKVYRRDNPSKGRYREFYQCD DIAG +E MGPDFE++KILTELLDEL IG+YE
Sbjct: 544 FKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDYE 603
Query: 606 IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIG 665
+KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+KKEMVEEKGL+ E+A+RIG
Sbjct: 604 VKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIG 663
Query: 666 NFVKERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLAR 725
NFVKE+G PL+LLSKL+QE S L N+ S +AL EL I+F AL++SKC +++VFDLSLAR
Sbjct: 664 NFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSLAR 723
Query: 726 GLDYYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQI 785
GLDYYTGVI+EA+ G +VGSI AGGR+DNLIGMFG+KQ+PAVG+SLGIERV IME++
Sbjct: 724 GLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIMEEL 783
Query: 786 LKDQNKAVRATKTDVLVSILGDD-LTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKES 845
+ Q + +R T+T VLVSI+ D+ L AAEL S++WGAK+N E+LV+KR KH +RAKES
Sbjct: 784 NEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKES 843
Query: 846 RIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 878
IPW+V +GE+E+S V LK + E E+ T +R ++ELKK L
Sbjct: 844 GIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match:
P93422 (Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150900 PE=2 SV=2)
HSP 1 Score: 743.8 bits (1919), Expect = 2.2e-213
Identity = 410/760 (53.95%), Postives = 533/760 (70.13%), Query Frame = 0
Query: 138 LDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKEEVGVANDMKVLLNGSKL 197
+D ++ L +ADAVA +SCEA++ DV+AF + SGDG ++K+E VA D+K+LL GSKL
Sbjct: 36 IDCCASPLVRVADAVAALSCEAARGDVAAFDVPTSGDGLSAKDEADVAADVKMLLFGSKL 95
Query: 198 VGR---IESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKGGSLSS--GTEDTTR 257
VG ++ + +K+P V+G RE + +H+R+R+ELN+ VK+GK ++ + G E+
Sbjct: 96 VGAAGGADAASFTKVPTVNGIFREAVRALHARVRIELNAPVKLGKRDAVQTGEGKEEALV 155
Query: 258 TALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSNESLKKEYKLVCE 317
A + + K S+ R +L + D ++ L + + LK ++ +
Sbjct: 156 ALATQLARPVQAMLKLSVARARLCVARIDDAELRKKLT-----DGVEIDDLK---GMLGK 215
Query: 318 LSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDVSTNEA------- 377
+++D + V L I +WEA A+ +IE I K Q NEA
Sbjct: 216 VTIDSDAVSVLRGVYNSLLKFRDILAWEAAVAMAVIEMDSSIEKPQACEENEAGSSTENP 275
Query: 378 ---NEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLV-----KDLLSFL 437
EK + KKK LGKGTS V+ L++D L NG + S+ + + +L
Sbjct: 276 HASGEKPKGDKKSKKKKTLGKGTSAVLMLLRD-LVTNGKEVLSVNSALLAEWGTELSLLF 335
Query: 438 DPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGA 497
DPK +L+ K+K+IVE+NE RRLPK+PKGTRDF KEQM IR+ AFSII VF+ HGA
Sbjct: 336 DPKCPRLVSLVDKVKEIVETNEVRRLPKIPKGTRDFGKEQMAIREHAFSIITGVFKMHGA 395
Query: 498 TTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKR 557
+LDTP FELRETL GKYGEDSKLI+DLADQGGEL SLRYDLTVPFARYVAMN ++S KR
Sbjct: 396 VSLDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNNISSLKR 455
Query: 558 YQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKL 617
YQIAKVYRRDNPSKGRYREFYQCD DIAG YE M PDFEVIK+LTELLD+L+IG YEIKL
Sbjct: 456 YQIAKVYRRDNPSKGRYREFYQCDFDIAGVYETMEPDFEVIKVLTELLDQLDIGTYEIKL 515
Query: 618 NHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFV 677
NHRKLLDGMLEICGVPPEKFRT+CSSIDKLDKQ+F+Q+KKE+V+EKG++ E A++IG+ V
Sbjct: 516 NHRKLLDGMLEICGVPPEKFRTVCSSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLV 575
Query: 678 KERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLD 737
K RG PL++L +L++E S + N GS AL+EL+ILF AL+K+ I K+VFDLSLARGLD
Sbjct: 576 KTRGPPLEVLLELRKEGSKFMGNAGSVTALNELEILFKALDKANAIGKIVFDLSLARGLD 635
Query: 738 YYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKD 797
YYTGVIYEA+FKG TQVGSIAAGGR+DNL+GMF KQ+PAVG+SLGIERV IMEQ +
Sbjct: 636 YYTGVIYEAVFKGTTQVGSIAAGGRYDNLVGMFSGKQVPAVGVSLGIERVFAIMEQQEME 695
Query: 798 QNKAVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPW 857
+N+ +RAT+T+VLVSI+G DL LAAEL SE+W A + EF + R+ H+ A +S IPW
Sbjct: 696 KNQ-IRATETEVLVSIIGKDLILAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSGIPW 755
Query: 858 IVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRL 878
+V +GE E+S G VKLKN+ +E + R+ LK++L
Sbjct: 756 MVLVGESEISSGKVKLKNLAASQEEEVDRTEFAQVLKQKL 785
BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match:
E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)
HSP 1 Score: 461.8 bits (1187), Expect = 1.7e-128
Identity = 244/457 (53.39%), Postives = 328/457 (71.77%), Query Frame = 0
Query: 445 KLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFD 504
K KGTRD+ +QM IR+K F+II + F+RHGA T+D+P FEL+ETLTGKYGEDSKLI+D
Sbjct: 57 KTAKGTRDYNPKQMAIREKVFNIIINCFKRHGAETIDSPVFELKETLTGKYGEDSKLIYD 116
Query: 505 LADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQIAKVYRRDNP--SKGRYREFYQCDL 564
L DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYRRDNP ++GRYREFYQCD
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176
Query: 565 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICS 624
DIAG Y+ M PD E +K++ E+L EL++G++ IK+N R++LDGM ICGVP EKFRTICS
Sbjct: 177 DIAGQYDAMIPDAECLKLVYEILSELDLGDFRIKVNDRRILDGMFAICGVPDEKFRTICS 236
Query: 625 SIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKG 684
++DKLDK +++++KKEMV EKGL+ EVA+RI ++V +G DL +L Q+ L Q+K
Sbjct: 237 TVDKLDKLAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGK-DLAERLLQDPK-LSQSKQ 296
Query: 685 SSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIF-------------- 744
+ ++++ +LFS LE + DKVVFDLSLARGLDYYTGVIYEAI
Sbjct: 297 ACAGITDMKLLFSYLELFQITDKVVFDLSLARGLDYYTGVIYEAILTQANPAPASTPAEQ 356
Query: 745 ----KGGTQVGSIAAGGRFDNLIGMFGSK--QIPAVGISLGIERVLVIMEQILKDQNKAV 804
G VGS+A GGR+D L+GMF K ++P VG+S+GIERV IMEQ + ++ V
Sbjct: 357 NGAEDAGVSVGSVAGGGRYDGLVGMFDPKGRKVPCVGVSIGIERVFSIMEQKAELSSEKV 416
Query: 805 RATKTDVLV-SILGDDLTLAAELASEMWGAKLNVEFLV--NKRVMKHIDRAKESRIPWIV 864
R T+T VLV S + L +L +E+W A + E L N +++ + +++ IP +
Sbjct: 417 RTTETQVLVASAQKNLLEERLKLTAELWNAGIKAEVLYKKNPKLLSQLQHCEDTGIPLVA 476
Query: 865 FLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKR 877
LGE+E+ +G+VKL+NV + EEV + R+ ++DE+KKR
Sbjct: 477 ILGEQELKDGVVKLRNVASREEVDVPRAELVDEVKKR 511
BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match:
Q2KI84 (Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1)
HSP 1 Score: 456.4 bits (1173), Expect = 7.1e-127
Identity = 238/447 (53.24%), Postives = 323/447 (72.26%), Query Frame = 0
Query: 445 KLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFD 504
K PKGTRD++ QM +R+K F +I S F+RHGA +DTP FEL+ETLTGKYGEDSKLI+D
Sbjct: 57 KTPKGTRDYSPRQMAVREKVFDVIISCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYD 116
Query: 505 LADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQIAKVYRRDNP--SKGRYREFYQCDL 564
L DQGGEL SLRYDLTVPFARY+AMN LT+ KRY IAKVYRRDNP ++GRYREFYQCD
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176
Query: 565 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICS 624
DIAG ++ M PD E +KI+ E+L L IG++ +K+N R++LDGM ICGVP KFRTICS
Sbjct: 177 DIAGQFDPMLPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVPDSKFRTICS 236
Query: 625 SIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKG 684
S+DKLDK S++++K EMV EKGL EVA+RIG++V++ G + L+ +L Q+ L QNK
Sbjct: 237 SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDPK-LSQNKQ 296
Query: 685 SSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIF-----KGGTQ---V 744
+ + L +L +LF L DK+ FDLSLARGLDYYTGVIYEA+ + G + V
Sbjct: 297 ALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQPPARAGEEPLGV 356
Query: 745 GSIAAGGRFDNLIGMFGSK--QIPAVGISLGIERVLVIMEQILKDQNKAVRATKTDVLV- 804
GS+AAGGR+D L+GMF K ++P VG+S+G+ER+ I+EQ L+ + VR T+T VLV
Sbjct: 357 GSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKVRTTETQVLVA 416
Query: 805 SILGDDLTLAAELASEMWGAKLNVEFLV--NKRVMKHIDRAKESRIPWIVFLGEREVSEG 864
S L +L SE+W A + E L N +++ + +E+ IP + +GE+E+ +G
Sbjct: 417 SAQKKLLEERLKLISELWDAGIKAELLYKKNPKLLNQLQYCEETGIPLVAIIGEQELKDG 476
Query: 865 IVKLKNVETFEEVTISRSNIIDELKKR 877
++KL++V + EEV + R ++++E+K+R
Sbjct: 477 VIKLRSVASREEVDVRREDLVEEIKRR 501
BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match:
Q86AS6 (Histidine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=hisS PE=3 SV=1)
HSP 1 Score: 452.6 bits (1163), Expect = 1.0e-125
Identity = 232/452 (51.33%), Postives = 317/452 (70.13%), Query Frame = 0
Query: 432 KDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETL 491
K+ E ++ K PKGT+D+ QMTIR++ F I+ VF+RHGA T++TP FEL+ETL
Sbjct: 27 KEKKEKKSNKVQLKTPKGTQDYNPRQMTIREQVFDGIKQVFKRHGAVTIETPVFELKETL 86
Query: 492 TGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQIAKVYRRDNP-- 551
TGKYGEDSKLI+DL DQGGE+ SLRYDLTVPFARYVAMNG+ + KRY IA+VYRRDNP
Sbjct: 87 TGKYGEDSKLIYDLQDQGGEICSLRYDLTVPFARYVAMNGVLNIKRYHIARVYRRDNPIM 146
Query: 552 SKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHRKLLDGMLEI 611
+KGR+REFYQCD DIAG Y+ M PD E + ++ E+L+++ +G+++IKLNHRKLLD + I
Sbjct: 147 TKGRFREFYQCDFDIAGTYDLMVPDAECLVMICEILEQVKVGDFQIKLNHRKLLDAIFAI 206
Query: 612 CGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSK 671
CGVP +KFR ICS++DKLDK +++++KEMVE K L VA++I FV + P+ LL
Sbjct: 207 CGVPADKFRAICSAVDKLDKSPWEEVRKEMVEVKALDGAVADKIEKFVSLKDEPIKLLQH 266
Query: 672 LKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFK 731
L+ NK +++ALS+L+ LF LE ++FDLSLARGLDYYTG+IYEA+
Sbjct: 267 LR-ATGMCDGNKDATEALSQLETLFGYLECFGVTQHILFDLSLARGLDYYTGIIYEAVLT 326
Query: 732 GGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNKAVRATKTDV 791
G +VGSIAAGGR+D L+GM+G K +PAVG S+GIER+ I+E K +NK +R T V
Sbjct: 327 GQDRVGSIAAGGRYDGLVGMYGKKDVPAVGFSIGIERIFTILEDEYKKENKKIRENATQV 386
Query: 792 LVSILGDDLTL-AAELASEMWGAKLNVEF--LVNKRVMKHIDRAKESRIPWIVFLGEREV 851
V + DL + SE+W A +N EF VN ++ ++ A ES IP I+ +G+ EV
Sbjct: 387 FVVQMEKDLIKERLAIVSELWKAGINAEFSYKVNPKLPAQLNTADESNIPLIIIIGKSEV 446
Query: 852 SEGIVKLKNVETFEEVTISRSNIIDELKKRLT 879
+ +K + ++V+I RSN ++K+ L+
Sbjct: 447 ETNSLSVKTMHDRKQVSIERSNFTVKIKEILS 477
BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match:
A0A0A0LSU1 (Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_2G401360 PE=3 SV=1)
HSP 1 Score: 1651.3 bits (4275), Expect = 0.0e+00
Identity = 867/879 (98.63%), Postives = 873/879 (99.32%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL
Sbjct: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLI ETLNSKPETLQFESLDVTNEE
Sbjct: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLITETLNSKPETLQFESLDVTNEE 120
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNGVCALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESL+KEYKLVCELSLDEKYDEFVHTVNVLL TVWKIFSWEATAALL IEGGELIGKGQDV
Sbjct: 301 ESLRKEYKLVCELSLDEKYDEFVHTVNVLLVTVWKIFSWEATAALLTIEGGELIGKGQDV 360
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGTSVVVQ IKD+LQGNGGGLGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVVQSIKDKLQGNGGGLGSLENLVKDLLSFLDPK 420
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRK+AFSIIESVFERHGATTL
Sbjct: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKQAFSIIESVFERHGATTL 480
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIGNFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGNFVKER 660
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
LGEREVSEGIVKLKNVETFEEVTISRS IIDELKKRLTP
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSTIIDELKKRLTP 879
BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match:
A0A5A7T4J4 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G001410 PE=3 SV=1)
HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN NAASIKHHL
Sbjct: 1 MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 60
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 61 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 120
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 360
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 420
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 660
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
VRATKTDVLVSILGDDLTLAAELASEMW KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 879
BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match:
A0A5D3C5F8 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00730 PE=3 SV=1)
HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN NAASIKHHL
Sbjct: 60 MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 119
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 120 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 179
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 180 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 239
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 240 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 299
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 300 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 359
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 360 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 419
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 420 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 479
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 480 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 539
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 540 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 599
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 600 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 659
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 660 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 719
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 720 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 779
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 780 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 839
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
VRATKTDVLVSILGDDLTLAAELASEMW KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 840 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 899
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 900 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 938
BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match:
A0A1S3B3G8 (Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103485391 PE=3 SV=1)
HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN NAASIKHHL
Sbjct: 1 MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 60
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 61 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 120
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 360
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 420
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 660
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
VRATKTDVLVSILGDDLTLAAELASEMW KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 879
BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match:
A0A6J1DGB6 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1)
HSP 1 Score: 1502.3 bits (3888), Expect = 0.0e+00
Identity = 785/884 (88.80%), Postives = 829/884 (93.78%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADP+EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSAL+RLSSSSN+ NAAS KH +
Sbjct: 1 MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
IP+FLTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVT+EE
Sbjct: 61 FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQS ALSSIADAVA ISCEAS+ADVSAF LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IE A SKIPK HGCLREQAKLVHS+MRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTED+ RTALLSFAA+LWDLGKCSL RGKLIL S+ +EN+KASL LL RECPSN
Sbjct: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESLKKEYKLV EL+LD++YDEFVHTVNVLL TVWKI +WEA AA L IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNG-----GGLGSLENLVKDLLS 420
NE NEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQG G GGLGSLEN VKDLLS
Sbjct: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420
Query: 421 FLDPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERH 480
FLDPKAS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERH
Sbjct: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480
Query: 481 GATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSF 540
GAT LDTPAFELRETL GKYGEDSKLI+DLADQGGEL SLRYDLTVPFARYVAMNG+TSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540
Query: 541 KRYQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEI 600
KRYQIAKVYRRDNPSKGR+REFYQCD DIAG YEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600
Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGN 660
KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVE+AE+IG+
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660
Query: 661 FVKERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARG 720
FVKERGHPL+LLSKLKQEQ LLQNKGS DAL EL+ILF+ALEKSKC+DKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720
Query: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQIL 780
LDYYTGVIYEA+FKGGTQVGSIAAGGR+DNLIGMFGSKQ+PAVG+SLGIERV VIMEQ+L
Sbjct: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780
Query: 781 KDQNKAVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRI 840
KDQN+ RATKT+VLVSILGDDLT AAELASE+W AKLN EFLVNKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840
Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884
BLAST of Chy3G050100 vs. NCBI nr
Match:
XP_004138662.1 (histidine--tRNA ligase, cytoplasmic [Cucumis sativus] >KGN63076.1 hypothetical protein Csa_021991 [Cucumis sativus])
HSP 1 Score: 1657 bits (4290), Expect = 0.0
Identity = 867/879 (98.63%), Postives = 873/879 (99.32%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL
Sbjct: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLI ETLNSKPETLQFESLDVTNEE
Sbjct: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLITETLNSKPETLQFESLDVTNEE 120
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNGVCALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESL+KEYKLVCELSLDEKYDEFVHTVNVLL TVWKIFSWEATAALL IEGGELIGKGQDV
Sbjct: 301 ESLRKEYKLVCELSLDEKYDEFVHTVNVLLVTVWKIFSWEATAALLTIEGGELIGKGQDV 360
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGTSVVVQ IKD+LQGNGGGLGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVVQSIKDKLQGNGGGLGSLENLVKDLLSFLDPK 420
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRK+AFSIIESVFERHGATTL
Sbjct: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKQAFSIIESVFERHGATTL 480
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIGNFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGNFVKER 660
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
LGEREVSEGIVKLKNVETFEEVTISRS IIDELKKRLTP
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSTIIDELKKRLTP 879
BLAST of Chy3G050100 vs. NCBI nr
Match:
TYK06605.1 (histidine--tRNA ligase [Cucumis melo var. makuwa])
HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN NAASIKHHL
Sbjct: 60 MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 119
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 120 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 179
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 180 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 239
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 240 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 299
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 300 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 359
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 360 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 419
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 420 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 479
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 480 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 539
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 540 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 599
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 600 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 659
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 660 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 719
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 720 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 779
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 780 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 839
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
VRATKTDVLVSILGDDLTLAAELASEMW KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 840 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 899
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 900 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 938
BLAST of Chy3G050100 vs. NCBI nr
Match:
XP_008441178.2 (PREDICTED: histidine--tRNA ligase, cytoplasmic [Cucumis melo] >KAA0037046.1 histidine--tRNA ligase [Cucumis melo var. makuwa])
HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN NAASIKHHL
Sbjct: 1 MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 60
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 61 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 120
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 360
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 420
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 660
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
VRATKTDVLVSILGDDLTLAAELASEMW KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 879
BLAST of Chy3G050100 vs. NCBI nr
Match:
XP_038884703.1 (histidine--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida])
HSP 1 Score: 1573 bits (4074), Expect = 0.0
Identity = 816/879 (92.83%), Postives = 851/879 (96.81%), Query Frame = 0
Query: 1 MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
MADPTEVSVITLGGKGSSLSS+SVYAIAHGFALVRIDSSALDRLSSSSN+ NAAS+KHH+
Sbjct: 1 MADPTEVSVITLGGKGSSLSSTSVYAIAHGFALVRIDSSALDRLSSSSNDQNAASLKHHV 60
Query: 61 LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
IPDFLTREEARA+LVVLLNKLIIS+SSGIRSVIPVLIA+ LNS+PET +FESLDVT+EE
Sbjct: 61 FIPDFLTREEARAALVVLLNKLIISASSGIRSVIPVLIADKLNSRPETFRFESLDVTDEE 120
Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
LSVFKQSCYVLNG+CALLDHQS ALSSIADAVAG+SCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAGMSCEASKADVSAFSLMDSGDGFASKE 180
Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
EVGVANDMKVLLNGSKLVG+IESEAISKIPKVHGCLREQAKLVHSR+RVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESEAISKIPKVHGCLREQAKLVHSRVRVELNSSVKIGKG 240
Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
GSLSSGTEDT RTALLSFAAMLWDLGKCSL+RGKLILGSSGD+NIKASLA LL RECPSN
Sbjct: 241 GSLSSGTEDTIRTALLSFAAMLWDLGKCSLERGKLILGSSGDDNIKASLAGLLDRECPSN 300
Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
ESLKKEY+LVCELSLDEK+DEFVHTVNVLL TVWKIF+WEATAALL IEGGEL+ KGQDV
Sbjct: 301 ESLKKEYRLVCELSLDEKHDEFVHTVNVLLVTVWKIFAWEATAALLTIEGGELMVKGQDV 360
Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
TNEANEKVVKKNEKKKKAVLGKGT+VVVQLIKDRLQG GGGLGSLENLVKDLLSFLDPK
Sbjct: 361 DTNEANEKVVKKNEKKKKAVLGKGTNVVVQLIKDRLQGKGGGLGSLENLVKDLLSFLDPK 420
Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASQFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480
Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
DTPAFELRETLTGKYGEDSKLIFDLADQGGEL SLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELCSLRYDLTVPFARYVAMNGLTSFKRYQI 540
Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
AKVYRRDNPSKGRYREFYQCD DIAG YEKMGPDFEVIKI+TELLDELNIG+YEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGQYEKMGPDFEVIKIMTELLDELNIGQYEIKLNHR 600
Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVE AERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVETAERIGDFVKER 660
Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
GHPLDLLSKLKQE+S LLQNKGSSDALS+L+ILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEESVLLQNKGSSDALSDLEILFSALEKSKCIDKVVFDLSLARGLDYYT 720
Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
GVIYEA+FKGGTQVGSIAAGGRFDNLIGMFGSKQ+PAVGISLGIERVLVIMEQ LKDQ +
Sbjct: 721 GVIYEAVFKGGTQVGSIAAGGRFDNLIGMFGSKQVPAVGISLGIERVLVIMEQNLKDQKQ 780
Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
RATKTD+LVSILGDDLTLAAELASEMW AKL+ EFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TARATKTDILVSILGDDLTLAAELASEMWSAKLHAEFLVNKRVMKHIDRAKESRIPWIVF 840
Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
LGEREVSEGIVKLKNVETFEE+TI RSNIIDELKKRLTP
Sbjct: 841 LGEREVSEGIVKLKNVETFEEITIPRSNIIDELKKRLTP 879
BLAST of Chy3G050100 vs. NCBI nr
Match:
XP_023550553.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1509 bits (3907), Expect = 0.0
Identity = 779/881 (88.42%), Postives = 830/881 (94.21%), Query Frame = 0
Query: 1 MADPT-EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHH 60
MADP E+SVITLGGKGSSL+S+SVYAIAHGFALVRIDSS+L+R+SSSSNNPNAASIKHH
Sbjct: 1 MADPAAELSVITLGGKGSSLASTSVYAIAHGFALVRIDSSSLERISSSSNNPNAASIKHH 60
Query: 61 LLIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNE 120
L +PDFLTREEARASLVVLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61 LFVPDFLTREEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120
Query: 121 ELSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASK 180
ELSVF+QSCY+LNG+CALLDHQS ALSSIADAVA ISCEAS+ADVSAFSLMDSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180
Query: 181 EEVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGK 240
EEVG+ANDMKV LNGSKLVG+IES AISKIPK HGCLREQAKLVHS+MRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240
Query: 241 GG-SLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECP 300
GG SLSSGTED RT LLSFAAMLWDLGKCSLDR KLI GS+GDEN+KASLA LL RECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLIWGSNGDENMKASLAGLLDRECP 300
Query: 301 SNESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQ 360
SNESLK+EY+LV EL LDEKY EFVH VNVL TVWKI +WEA AA + IEGGELIGKGQ
Sbjct: 301 SNESLKREYRLVSELGLDEKYVEFVHAVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360
Query: 361 DVSTNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLD 420
DV N ANEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQG GGGLGSLENLVKDLLSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420
Query: 421 PKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGAT 480
PKASGFDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGAT
Sbjct: 421 PKASGFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480
Query: 481 TLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRY 540
LDTPAFELRETL GKYGEDSKL++DLADQGGEL SLRYDLTVPFARYVAMNG+TSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540
Query: 541 QIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLN 600
QIAKVYRRDNPSKGR+REFYQCD DIAG YEKMGPDFEVIKILTELLDELNIG YEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGHYEIKLN 600
Query: 601 HRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVK 660
HR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVE+AERIG+FVK
Sbjct: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660
Query: 661 ERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDY 720
ERGHPL+LLSKLKQEQS LLQNKGSSDAL+EL+ILFSALEKS C+DKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720
Query: 721 YTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQ 780
YTGVIYEA+FKGGTQ+GSIAAGGR+DNLIGMFGSKQ+PAVG+SLGIERV VIMEQ+LKDQ
Sbjct: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780
Query: 781 NKAVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWI 840
N+ RATKT+VLVSILGDDLT AAELASE+W AK++ EFLVNKRVMKHIDRAKE+RIPWI
Sbjct: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840
Query: 841 VFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
VFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRLTP
Sbjct: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 881
BLAST of Chy3G050100 vs. TAIR 10
Match:
AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 981.5 bits (2536), Expect = 4.5e-286
Identity = 536/888 (60.36%), Postives = 659/888 (74.21%), Query Frame = 0
Query: 6 EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHLL-IPD 65
E S ITLGGKGSSLSSSSVY +A G A VRIDSSA++R S+ N SIK IP
Sbjct: 4 ERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGIPQ 63
Query: 66 FLTREEARASLVVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTNE 125
LT EE RASL VLLNKLI+S SSS RSV+P+ I E LN K E+L+ +DVT
Sbjct: 64 GLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTEG 123
Query: 126 ELSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASK 185
E V ++SC L G+C+++DH+ST LS I D+VA +SCE +KAD+++FSL+DSGDG K
Sbjct: 124 ENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGDK 183
Query: 186 EEVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGK 245
+ +GVA D+KVLLNG K G++E E ISKIP +HG R K VH+ R ELNS VK GK
Sbjct: 184 DVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGGK 243
Query: 246 GGSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPS 305
GS ++G + T LL + +LG CS R KL DEN+K L+ + C
Sbjct: 244 TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLSEKI---CVE 303
Query: 306 NESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQD 365
NE+LK YKL L+E Y F H +N L VW+I EA AA + GGEL +
Sbjct: 304 NENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKSG 363
Query: 366 VSTNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGN-----GGGLGSLENLVKDLL 425
+ E ++ KK + +KKAV+GKGTS+V+Q IKDRL N G + SL + +L
Sbjct: 364 DADKEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQIL 423
Query: 426 SFLDPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFER 485
+ +P+ FD+LL K+K+IVESNE+RRLPKLPKGTRDFAKEQM +R+KAFSII++VF+R
Sbjct: 424 NLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVFKR 483
Query: 486 HGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTS 545
HGAT LDTP FELRETL GKYGEDSKL++D+ADQGGEL SLRYDLTVPFARYVAMNG+TS
Sbjct: 484 HGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGITS 543
Query: 546 FKRYQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYE 605
FKRYQIAKVYRRDNPSKGRYREFYQCD DIAG +E MGPDFE++KILTELLDEL IG+YE
Sbjct: 544 FKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDYE 603
Query: 606 IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIG 665
+KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+KKEMVEEKGL+ E+A+RIG
Sbjct: 604 VKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIG 663
Query: 666 NFVKERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLAR 725
NFVKE+G PL+LLSKL+QE S L N+ S +AL EL I+F AL++SKC +++VFDLSLAR
Sbjct: 664 NFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSLAR 723
Query: 726 GLDYYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQI 785
GLDYYTGVI+EA+ G +VGSI AGGR+DNLIGMFG+KQ+PAVG+SLGIERV IME++
Sbjct: 724 GLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIMEEL 783
Query: 786 LKDQNKAVRATKTDVLVSILGDD-LTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKES 845
+ Q + +R T+T VLVSI+ D+ L AAEL S++WGAK+N E+LV+KR KH +RAKES
Sbjct: 784 NEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKES 843
Query: 846 RIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 878
IPW+V +GE+E+S V LK + E E+ T +R ++ELKK L
Sbjct: 844 GIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883
BLAST of Chy3G050100 vs. TAIR 10
Match:
AT3G46100.1 (Histidyl-tRNA synthetase 1 )
HSP 1 Score: 157.9 bits (398), Expect = 3.7e-38
Identity = 122/432 (28.24%), Postives = 218/432 (50.46%), Query Frame = 0
Query: 447 PKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSK-LIFDL 506
PKGTRDF E M +R F+ + V +G +D P E K GE+ + ++
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCF 131
Query: 507 ADQGGELYSLRYDLTVPFARYVAMNGLT---SFKRYQIAKVYRRDNPSKGRYREFYQCDL 566
D+G +LR +LT AR V G + K + I + +R + ++GR RE YQ ++
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 191
Query: 567 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEI--KLNHRKLLDGMLEICGVPPEKFRTI 626
DI G ++ + E+I + + I ++ K++ RK+L +L+ GVP + F +
Sbjct: 192 DIIG-VPQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRV 251
Query: 627 CSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQN 686
C IDK++K D+IKKE+ G++ + E++ + + LD L +
Sbjct: 252 CIIIDKIEKIPIDEIKKEL-GFTGISEDAIEQLLQVLSVKS--LDDLEDI---------I 311
Query: 687 KGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVGSIAAG 746
G+ +A+++L LFS EK + + FD S+ RGL YYTG+++E + G ++ +I G
Sbjct: 312 GGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKG-KLRAICGG 371
Query: 747 GRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNKAVR-ATKTDVLVSILGDDLT 806
GR+D L+ +G PA G G + V+V ++LK+++ + + +V L DL
Sbjct: 372 GRYDRLLSTYGGDDFPACGFGFG-DAVIV---ELLKEKDLLPELGQEVENIVCALDKDLQ 431
Query: 807 -LAAELASEMWGAKLNVEFLVNKRVMKHI-DRAKESRIPWIVFLGEREVSEGIVKLKNVE 866
AA +A+ + V+ ++ + +K + RA +V +G+ E +G V +K +
Sbjct: 432 GAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLS 485
Query: 867 TFEEVTISRSNI 870
+ E+ + S++
Sbjct: 492 SGEQFQVKLSDL 485
BLAST of Chy3G050100 vs. TAIR 10
Match:
AT3G59410.1 (protein kinase family protein )
HSP 1 Score: 107.5 bits (267), Expect = 5.7e-23
Identity = 110/445 (24.72%), Postives = 196/445 (44.04%), Query Frame = 0
Query: 460 IRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDL 519
+R I + VF +H A L+ + L + S+ L GG++ L Y+L
Sbjct: 796 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 855
Query: 520 TVPFARYVAMNGLTSFKRYQIAKVYRR------DNPSKGRYREFYQCDLDIAGHYEKMGP 579
+PF ++++N +SFKRY+I+ VYRR NP Q D DI G +
Sbjct: 856 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 915
Query: 580 DFEVIKILTELLDEL-NIGEYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 639
+ EV+K++ ++ + + G +I LNH LLD + G+ E K + S + L
Sbjct: 916 EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 975
Query: 640 QSFDQ------IKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKGS 699
QS ++ I++++++E L V R+ D L + + AL ++ +
Sbjct: 976 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDAD--QALPRLRGALRADRPT 1035
Query: 700 SDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVGS------I 759
AL EL L + L + + V D+ + Y+ + ++ G+ +
Sbjct: 1036 RKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLL 1095
Query: 760 AAGGRFDNLIGMFGSKQ----IP-AVGISLGIERVLVIMEQILKDQNKAVRATKTDVLVS 819
A GGR+D L+ ++ +P AVG+SL +E I + + D T VLV
Sbjct: 1096 AVGGRYDWLVQEVCDREHKMNLPGAVGVSLALE---TIFQHLPMDLRPIRNEVSTSVLVC 1155
Query: 820 ILGDD--LTLAAELASEMWGAKLNVEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVSE 871
G L EL +E+W + EF+ + + + + A E I +V + E V++
Sbjct: 1156 SRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQ 1215
BLAST of Chy3G050100 vs. TAIR 10
Match:
AT3G59410.2 (protein kinase family protein )
HSP 1 Score: 107.5 bits (267), Expect = 5.7e-23
Identity = 110/445 (24.72%), Postives = 196/445 (44.04%), Query Frame = 0
Query: 460 IRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDL 519
+R I + VF +H A L+ + L + S+ L GG++ L Y+L
Sbjct: 820 LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 879
Query: 520 TVPFARYVAMNGLTSFKRYQIAKVYRR------DNPSKGRYREFYQCDLDIAGHYEKMGP 579
+PF ++++N +SFKRY+I+ VYRR NP Q D DI G +
Sbjct: 880 RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 939
Query: 580 DFEVIKILTELLDEL-NIGEYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 639
+ EV+K++ ++ + + G +I LNH LLD + G+ E K + S + L
Sbjct: 940 EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 999
Query: 640 QSFDQ------IKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKGS 699
QS ++ I++++++E L V R+ D L + + AL ++ +
Sbjct: 1000 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDAD--QALPRLRGALRADRPT 1059
Query: 700 SDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVGS------I 759
AL EL L + L + + V D+ + Y+ + ++ G+ +
Sbjct: 1060 RKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLL 1119
Query: 760 AAGGRFDNLIGMFGSKQ----IP-AVGISLGIERVLVIMEQILKDQNKAVRATKTDVLVS 819
A GGR+D L+ ++ +P AVG+SL +E I + + D T VLV
Sbjct: 1120 AVGGRYDWLVQEVCDREHKMNLPGAVGVSLALE---TIFQHLPMDLRPIRNEVSTSVLVC 1179
Query: 820 ILGDD--LTLAAELASEMWGAKLNVEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVSE 871
G L EL +E+W + EF+ + + + + A E I +V + E V++
Sbjct: 1180 SRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQ 1239
BLAST of Chy3G050100 vs. TAIR 10
Match:
AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )
HSP 1 Score: 85.5 bits (210), Expect = 2.3e-16
Identity = 56/187 (29.95%), Postives = 92/187 (49.20%), Query Frame = 0
Query: 447 PKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSK-LIFDL 506
PKGTRDF E M +R F+ + V G +D P E K GE+ + ++
Sbjct: 72 PKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQLYCF 131
Query: 507 ADQGGELYSLRYDLTVPFARYVAMNGLT---SFKRYQIAKVYRRDNPSKGRYREFYQCDL 566
D+G +LR +LT AR V G + K + + + +R + ++GR RE YQ ++
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHYQWNM 191
Query: 567 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEI--KLNHRKLLDGMLEICGVPPEKFRTI 626
DI G ++ + E+I + + I ++ K++ RK+L L+ GVP + F +
Sbjct: 192 DIIG-VPEVTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLFGRV 251
Query: 627 CSSIDKL 628
C IDK+
Sbjct: 252 CFIIDKV 257
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
F4IYF8 | 6.3e-285 | 60.36 | Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760... | [more] |
P93422 | 2.2e-213 | 53.95 | Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=... | [more] |
E9QI36 | 1.7e-128 | 53.39 | Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2 | [more] |
Q2KI84 | 7.1e-127 | 53.24 | Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1 | [more] |
Q86AS6 | 1.0e-125 | 51.33 | Histidine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=hisS... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LSU1 | 0.0e+00 | 98.63 | Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_2G401360 PE=3 SV=1 | [more] |
A0A5A7T4J4 | 0.0e+00 | 96.59 | Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... | [more] |
A0A5D3C5F8 | 0.0e+00 | 96.59 | Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... | [more] |
A0A1S3B3G8 | 0.0e+00 | 96.59 | Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103485391 PE=3 SV=1 | [more] |
A0A6J1DGB6 | 0.0e+00 | 88.80 | Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_004138662.1 | 0.0 | 98.63 | histidine--tRNA ligase, cytoplasmic [Cucumis sativus] >KGN63076.1 hypothetical p... | [more] |
TYK06605.1 | 0.0 | 96.59 | histidine--tRNA ligase [Cucumis melo var. makuwa] | [more] |
XP_008441178.2 | 0.0 | 96.59 | PREDICTED: histidine--tRNA ligase, cytoplasmic [Cucumis melo] >KAA0037046.1 hist... | [more] |
XP_038884703.1 | 0.0 | 92.83 | histidine--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida] | [more] |
XP_023550553.1 | 0.0 | 88.42 | histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo] | [more] |