Chy3G050100 (gene) Cucumber (hystrix) v1

Overview
NameChy3G050100
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionHistidine--tRNA ligase
LocationchrH03: 372969 .. 378969 (+)
RNA-Seq ExpressionChy3G050100
SyntenyChy3G050100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGACCCTACTGAGGTCTCCGTGATTACGCTCGGAGGAAAGGGTTCATCGCTTTCATCTTCCTCTGTCTATGCCATTGCTCATGGCTTCGCTCTTGTTCGGATTGACTCATCGGCCCTCGACAGGCTTTCTTCTTCATCCAACAATCCAAATGCTGCATCCATTAAGCACCACCTCCTCATTCCCGATTTTCTAACTCGCGAAGAAGCCAGGGCGTCCCTTGTTGTCCTCCTAAACAAGCTTATCATTTCTAGCTCTTCAGGGATTCGTTCGGTTATTCCTGTTCTAATTGCGGAGACTCTCAATTCGAAACCAGAAACTTTACAATTTGAATCACTTGATGTGACCAATGAGGAGTTATCCGTTTTCAAGCAATCGTGTTATGTTTTGAATGGAGTTTGCGCGTTGTTGGACCATCAATCAACAGCATTATCTTCCATTGCCGATGCTGTGGCTGGCATATCTTGCGAGGCGTCTAAAGCTGATGTGTCTGCGTTTAGTTTGATGGACTCGGGTGATGGGTTCGCCTCGAAGGAGGAGGTAGGAGTCGCCAATGATATGAAGGTACTTCTAAATGGATCTAAGTTGGTGGGTAGGATTGAGAGTGAAGCTATCTCTAAGATTCCAAAGGTTCATGGATGCTTAAGAGAGCAAGCGAAGTTGGTGCACTCAAGAATGCGGGTTGAGTTGAATTCTAGTGTGAAAATTGGGAAAGGAGGATCTTTGAGTTCTGGGACAGAGGATACCACTCGGACCGCATTGTTGTCATTTGCAGCCATGTTGTGGGACTTGGGAAAATGTAGTTTGGACAGAGGAAAGTTGATTTTGGGTTCAAGTGGCGACGAGAATATAAAGGCAAGTTTGGCGAGTTTGCTTGCTAGGGAATGCCCTAGTAATGAGAGTCTAAAAAAGGAATACAAACTGGTCTGCGAATTGTCTTTGGATGAGAAGTACGATGAATTTGTCCATACTGTGAATGTGTTGTTGGAAACTGTATGGAAAATTTTCTCTTGGGAGGCTACAGCAGCTCTCTTGATAATTGAAGGTGGTGAGTTGATAGGTAAGGGTCAAGATGTTAGCACGAATGAAGCTAACGAGAAGGTTGTAAAAAAGAATGAGAAGAAGAAGAAAGCAGTATTGGGTAAAGGGACTAGTGTGGTTGTTCAATTGATTAAAGATAGATTGCAGGGTAACGGCGGTGGTCTGGGGTCATTGGAGAACTTGGTAAAAGATCTTTTATCCTTTTTGGATCCCAAGGCTTCCGGGTTTGATAATCTATTGAAGAAAATCAAAGATATAGTTGAAAGCAACGAAAGTAGGAGGCTACCGAAGCTTCCCAAGGTAATGACATATGTCAAAACTTCACCTAAATTCCAAGTTATTTAGCCTTCCTCTTGTTTTGGTCAAGGAGGTGGACTCTCATTAGAAGAATATTATCATCCTTTGTTATAGCTTCTGTTTCCGCCGTTATTTCTTCAATTGCATATGCCCCCACATATTTTAGGTTATGCTAACAATGGAATCATACTTTTACCATCCGTTAAAAAATGTTAAAAATAGAGGCTTTACTAAGTATTTATTTAATACCCCATCAGCAAATACACATCAAAATAACTACAATTATCCATATTACATTTTAATTATTTGGTTCGTTCTATGTCACCTTTGTAATTATCGTAGATAGTTCCTTCGACCACTTAAAATTTTTGTAACACATGATATTGTTACTCAAACTGAATCGCTGAAAAGTGATCATTCTATTTGTTTGATTGTGTGTGTTTTTTTTTTAATATTATGCTTCTTATCCTTTTTTTAGAAAAAAACAAAAAAAAAATTATGCCTTATTTTAGTAGCTTGTTTTGAAAACATTGCGGGCAGTAGTTGAAAGGGAACAATATTTTACTGTCAAGCACTAGATACACACAGACACACACACATATAGAATCCAACTGTGCTTACTTATGATTCTCTGTGTACCGTCAATATGATGACAATAGTTTTCTTTCTCTGTAGTCACAAATCAGTATGCCATGTCTTGCTATTCAACTTCCTTACCATTCCAAGGCATATTTGTTAATATTTTTATTTTTGGATAAGACTGAAAGCTTTGGTCTGCTTTAAGTAGAATTTTTGTTTCCAAAATGAGCATAGTTCAATTGACGTAGTGGTGGTACACTATGAACCTTGAAGTCAGAGGTTTGCTTCCCCACCCTATATATTGTGAACAAAAGTAACATCTTCGTTTATTTATTTGTTTTTTTTGTGAGTTGGTTAATGGCAAAGGCTCATGAAGATCGATTGTAACTGCTCGCACCAATTATATTTTATGAATGGAACTGGAGAACTCTTTGTTTCTTTTAGAAGTCTCAAATTTAGAAAATAATATGGAAAGCAAAACATTCATTTTCGTTTGCTAGGTTAACGATATAGGCTTGTGCATTGTTTTATTTGTGCTTCTGCTTAACTCTTTTGTTAATTCTGTTACCCTTTGGTTTGGTTTAATATTTTATTGTTTTTTTTTTCTCTTTCTAACTCGAGTAAGGTGTTTGTTAATTTAACTCATTTGCTGTTGCTTACATGTTAGGGGACTCGGGATTTTGCGAAAGAGCAAATGACAATTAGAAAGAAAGCATTTTCAATTATAGAGAGCGTTTTTGAGCGCCATGGTGCCACAACCTTGGATACTCCAGCTTTTGAGTTGAGAGAAACTCTCACAGGGAAATATGGAGAAGATTCCAAGCTGATTTTTGATCTGGCCGATCAGGTTATAATAGATGTTCTTACTTCTGCTAATGGAACCACCAGTATCTTTCTTTTCAAATTTTAAAAGATATAATTGTTGGGCTGTTTAGTTAAAATTATTCCTCCCTTCATCCTAAGCATCTATTCATGTTTCGTGAAATAATTACATAATTACCAATTAACTGAATTATTTTTATCTGTCGCTGAGACTAATGATAGTTGATTGTTGATGTTTTTGGAACTTTATGTTTAAGTAGTCCTTGTATCTGTTGCCTTATTAATGCTATTTAAAAGCATGACTTTAAGTGATGTTGAGCTTTTTCGGTAATGTTAACATTCAATCCTTATGTCAAGGTTCTCTAATCCTTTCGGTATTAAGTAATCAATTCATTTTATTGAATCAATCCTTCTCTGTGTAAGCATTGGCTACTTCACCTTCCCTATTCTATCTAGTTTTAAGGTTTCTCAATGAATGACGTCGCTTAAATTTGCTCAACACTGAGTAGTTTTGTGTGACAGATCAATTACTCTTTGTTCTTCTTTCTCTGGTTCTCCATCATTGTAATTTCTTTCACAGTTTGTTTACAGGGTGGGGAGCTCTATTCGCTTAGATACGACTTGACTGTTCCATTTGCTAGGTATGTGGCCATGAATGGTTTAACATCATTCAAAAGGTATCAAATAGCTAAAGTTTATAGAAGGGACAACCCATCTAAAGGAAGATACCGTGAATTTTATCAATGTGATTTGGATATTGCTGGTCACTATGAAAAGATGGGGCCAGATTTTGAGGTTATCAAGATACTAACAGAGCTACTTGATGAACTGAACATTGGGGAATACGAGGTAACCTTTTTGATTAATTGTTCTGCTGAACACAACTCATTGTTTTTTGTTTGTTTGATGTCTATTTTTATGAACTTTTAGTTATTTTGAATAATTATGATGCCTCAGCAACTACATCGCATCGCAGAACAACAAACAAATATATTATAAGTGCATTTGAGTGGATTGGATTCCTAGATTCCCTTTCTATAACATTGAGTTGCTTCAGTCTATTCATATTGTTACCATTAACCTAATTTATTTTGTACCTATTCTCTTTTGCAGATAAAATTGAATCACCGAAAGCTATTGGATGGAATGTTAGAAATTTGTGGAGTACCACCTGAAAAGTTTAGAACCATATGTTCTAGTATTGACAAGTTAGACAAGCAATCGTTTGACCAGATAAAAAAAGAAATGGTAATTTAAATAAACTGTCGATCACAATCTTATTATTAAGCACGCCTGCCTCTTTCTGCATTCCCTTCGTAATATCTTTAGTTTTTTTAAATTATGTCTTCTAACATATTTACATTATTGCATTTCACCTGGGCCAAAACTCTTTGATAAACTCTTACATTAATCCTTTTTCCTAGTTCTGCATTATTTGTCATCCATCGTGGCCGTAACAATAATGGAATTATTGCACTTGATTATAATTAACTTTTACTTGCTAGGTGGAGGAAAAGGGGTTGACCGTGGAGGTAGCTGAAAGAATTGGTAATTTTGTGAAGGAAAGGGGACATCCATTAGACTTATTATCTAAGCTTAAACAGGAGCAAAGCGCGTTACTACAAAATAAAGGATCTTCTGATGCATTGAGTGAATTAGATATTTTGTTCAGTGCTCTAGAAAAGTCAAAGTGTATTGATAAAGTGGTATTTGACTTGAGTCTTGCCAGAGGACTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGCACCCAGGTAAGTTTCTATATGTTATAATCATGCCTGTCTCTACCATTTTTGTTTTTGACTTGTTAGGCTACTGACCATGGAGTTATTGGCATTGACTTGTTATTGCTACTTGTTTCAAGATCCTGTCGTTGCTCATTATGAATAAGTAACTGTTTGTTGTTGTGGATGGTAACGGATCAACTTCTTTATGTATTTGTGAAATTTACACCAGGAGAAGTATTTTGAGGCATGTAATTGTTTTTAGTTATGCAACAATATGATACTTCGTTGACTTCTGATGACAATTCTTTCTTCTATCTAAGATTATTTCTCCTTGGTTACATTACCTGCAATGATGTATAAAGCTATCAACGTGTTATCTTGTTGCCCACTAATACATATTTTATTTGGATTCTTTGAGGATTTAATTAGCAAATCATTTATATTCTCAGGTTGGATCTATTGCTGCTGGTGGACGCTTTGACAATCTGATTGGAATGTTTGGCTCCAAGCAGATCCCAGCAGTTGGTATCAGTCTTGGTATTGAGCGAGTTCTTGTTATTATGGAACAAATTTTGAAAGATCAAAACAAGGTAACGAGTTTGATTTTGTTTGCTGACCCTTTGATTTTTTATGGGTTTTTTTCCAGACCGTTTCTTTACAATGGTATTAACCTTTCTTTAAGGCAAAATTTCAACATTTTATGGTTTTCTTTTTTTTTTTTAGGCTAGAAATTAATTGTAGTCTCAAAATATGAAAAGGATTTTGAAAACATGGTTCAGAGGTAAATAGCAAATAAAGAAACTCATGATGGTGGGAGTAGTGTTGTTTAGAAGCTTAATTTTCAAAAACCAAAAACGAAAAATGAAATGTTTATCAATGGAGATTTTAGAGCTGATTTTAGAGGTATGTGCTTCTCCTGTAGTTAAACGTTAAAATGAAACTCAAAACTCTAAGTCATCACATTTCCACAATACCACCATTAAGGTTTGGAGATAAAAAATTTAGGGTGGATTTAGTTTAACTTTCAAGTATAAAATAAGTCATTGGAAAAGTTGAAAAACTTTTTTGAAACTCTCAAAGTGTACTACACTTTTAAATAGTTTCTATCCAAGGAGATTATATAAAAATGACTTTTTTGAAAAACATTTTTTTTAGTCAATCCAAACAAGCCCTATTCAACTGTTGGGATTTTGCAGGCTGTTCGGGCAACTAAGACTGATGTTTTAGTGAGCATACTGGGGGATGATTTGACACTAGCAGCAGAGCTTGCAAGTGAGATGTGGGGTGCGAAACTTAACGTTGAATTCCTGGTCAATAAAAGAGTGATGAAACATATCGATCGTGCGAAAGAGTCGAGAATCCCTTGGATAGTATTTTTAGGGGAACGTGAAGTGAGTGAAGGGATTGTGAAGTTGAAAAACGTGGAAACCTTTGAAGAAGTAACAATTTCCAGAAGCAACATCATTGACGAACTCAAGAAACGCCTCACTCCTTGA

mRNA sequence

ATGGCAGACCCTACTGAGGTCTCCGTGATTACGCTCGGAGGAAAGGGTTCATCGCTTTCATCTTCCTCTGTCTATGCCATTGCTCATGGCTTCGCTCTTGTTCGGATTGACTCATCGGCCCTCGACAGGCTTTCTTCTTCATCCAACAATCCAAATGCTGCATCCATTAAGCACCACCTCCTCATTCCCGATTTTCTAACTCGCGAAGAAGCCAGGGCGTCCCTTGTTGTCCTCCTAAACAAGCTTATCATTTCTAGCTCTTCAGGGATTCGTTCGGTTATTCCTGTTCTAATTGCGGAGACTCTCAATTCGAAACCAGAAACTTTACAATTTGAATCACTTGATGTGACCAATGAGGAGTTATCCGTTTTCAAGCAATCGTGTTATGTTTTGAATGGAGTTTGCGCGTTGTTGGACCATCAATCAACAGCATTATCTTCCATTGCCGATGCTGTGGCTGGCATATCTTGCGAGGCGTCTAAAGCTGATGTGTCTGCGTTTAGTTTGATGGACTCGGGTGATGGGTTCGCCTCGAAGGAGGAGGTAGGAGTCGCCAATGATATGAAGGTACTTCTAAATGGATCTAAGTTGGTGGGTAGGATTGAGAGTGAAGCTATCTCTAAGATTCCAAAGGTTCATGGATGCTTAAGAGAGCAAGCGAAGTTGGTGCACTCAAGAATGCGGGTTGAGTTGAATTCTAGTGTGAAAATTGGGAAAGGAGGATCTTTGAGTTCTGGGACAGAGGATACCACTCGGACCGCATTGTTGTCATTTGCAGCCATGTTGTGGGACTTGGGAAAATGTAGTTTGGACAGAGGAAAGTTGATTTTGGGTTCAAGTGGCGACGAGAATATAAAGGCAAGTTTGGCGAGTTTGCTTGCTAGGGAATGCCCTAGTAATGAGAGTCTAAAAAAGGAATACAAACTGGTCTGCGAATTGTCTTTGGATGAGAAGTACGATGAATTTGTCCATACTGTGAATGTGTTGTTGGAAACTGTATGGAAAATTTTCTCTTGGGAGGCTACAGCAGCTCTCTTGATAATTGAAGGTGGTGAGTTGATAGGTAAGGGTCAAGATGTTAGCACGAATGAAGCTAACGAGAAGGTTGTAAAAAAGAATGAGAAGAAGAAGAAAGCAGTATTGGGTAAAGGGACTAGTGTGGTTGTTCAATTGATTAAAGATAGATTGCAGGGTAACGGCGGTGGTCTGGGGTCATTGGAGAACTTGGTAAAAGATCTTTTATCCTTTTTGGATCCCAAGGCTTCCGGGTTTGATAATCTATTGAAGAAAATCAAAGATATAGTTGAAAGCAACGAAAGTAGGAGGCTACCGAAGCTTCCCAAGGGGACTCGGGATTTTGCGAAAGAGCAAATGACAATTAGAAAGAAAGCATTTTCAATTATAGAGAGCGTTTTTGAGCGCCATGGTGCCACAACCTTGGATACTCCAGCTTTTGAGTTGAGAGAAACTCTCACAGGGAAATATGGAGAAGATTCCAAGCTGATTTTTGATCTGGCCGATCAGGGTGGGGAGCTCTATTCGCTTAGATACGACTTGACTGTTCCATTTGCTAGGTATGTGGCCATGAATGGTTTAACATCATTCAAAAGGTATCAAATAGCTAAAGTTTATAGAAGGGACAACCCATCTAAAGGAAGATACCGTGAATTTTATCAATGTGATTTGGATATTGCTGGTCACTATGAAAAGATGGGGCCAGATTTTGAGGTTATCAAGATACTAACAGAGCTACTTGATGAACTGAACATTGGGGAATACGAGATAAAATTGAATCACCGAAAGCTATTGGATGGAATGTTAGAAATTTGTGGAGTACCACCTGAAAAGTTTAGAACCATATGTTCTAGTATTGACAAGTTAGACAAGCAATCGTTTGACCAGATAAAAAAAGAAATGGTGGAGGAAAAGGGGTTGACCGTGGAGGTAGCTGAAAGAATTGGTAATTTTGTGAAGGAAAGGGGACATCCATTAGACTTATTATCTAAGCTTAAACAGGAGCAAAGCGCGTTACTACAAAATAAAGGATCTTCTGATGCATTGAGTGAATTAGATATTTTGTTCAGTGCTCTAGAAAAGTCAAAGTGTATTGATAAAGTGGTATTTGACTTGAGTCTTGCCAGAGGACTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGCACCCAGGTTGGATCTATTGCTGCTGGTGGACGCTTTGACAATCTGATTGGAATGTTTGGCTCCAAGCAGATCCCAGCAGTTGGTATCAGTCTTGGTATTGAGCGAGTTCTTGTTATTATGGAACAAATTTTGAAAGATCAAAACAAGGCTGTTCGGGCAACTAAGACTGATGTTTTAGTGAGCATACTGGGGGATGATTTGACACTAGCAGCAGAGCTTGCAAGTGAGATGTGGGGTGCGAAACTTAACGTTGAATTCCTGGTCAATAAAAGAGTGATGAAACATATCGATCGTGCGAAAGAGTCGAGAATCCCTTGGATAGTATTTTTAGGGGAACGTGAAGTGAGTGAAGGGATTGTGAAGTTGAAAAACGTGGAAACCTTTGAAGAAGTAACAATTTCCAGAAGCAACATCATTGACGAACTCAAGAAACGCCTCACTCCTTGA

Coding sequence (CDS)

ATGGCAGACCCTACTGAGGTCTCCGTGATTACGCTCGGAGGAAAGGGTTCATCGCTTTCATCTTCCTCTGTCTATGCCATTGCTCATGGCTTCGCTCTTGTTCGGATTGACTCATCGGCCCTCGACAGGCTTTCTTCTTCATCCAACAATCCAAATGCTGCATCCATTAAGCACCACCTCCTCATTCCCGATTTTCTAACTCGCGAAGAAGCCAGGGCGTCCCTTGTTGTCCTCCTAAACAAGCTTATCATTTCTAGCTCTTCAGGGATTCGTTCGGTTATTCCTGTTCTAATTGCGGAGACTCTCAATTCGAAACCAGAAACTTTACAATTTGAATCACTTGATGTGACCAATGAGGAGTTATCCGTTTTCAAGCAATCGTGTTATGTTTTGAATGGAGTTTGCGCGTTGTTGGACCATCAATCAACAGCATTATCTTCCATTGCCGATGCTGTGGCTGGCATATCTTGCGAGGCGTCTAAAGCTGATGTGTCTGCGTTTAGTTTGATGGACTCGGGTGATGGGTTCGCCTCGAAGGAGGAGGTAGGAGTCGCCAATGATATGAAGGTACTTCTAAATGGATCTAAGTTGGTGGGTAGGATTGAGAGTGAAGCTATCTCTAAGATTCCAAAGGTTCATGGATGCTTAAGAGAGCAAGCGAAGTTGGTGCACTCAAGAATGCGGGTTGAGTTGAATTCTAGTGTGAAAATTGGGAAAGGAGGATCTTTGAGTTCTGGGACAGAGGATACCACTCGGACCGCATTGTTGTCATTTGCAGCCATGTTGTGGGACTTGGGAAAATGTAGTTTGGACAGAGGAAAGTTGATTTTGGGTTCAAGTGGCGACGAGAATATAAAGGCAAGTTTGGCGAGTTTGCTTGCTAGGGAATGCCCTAGTAATGAGAGTCTAAAAAAGGAATACAAACTGGTCTGCGAATTGTCTTTGGATGAGAAGTACGATGAATTTGTCCATACTGTGAATGTGTTGTTGGAAACTGTATGGAAAATTTTCTCTTGGGAGGCTACAGCAGCTCTCTTGATAATTGAAGGTGGTGAGTTGATAGGTAAGGGTCAAGATGTTAGCACGAATGAAGCTAACGAGAAGGTTGTAAAAAAGAATGAGAAGAAGAAGAAAGCAGTATTGGGTAAAGGGACTAGTGTGGTTGTTCAATTGATTAAAGATAGATTGCAGGGTAACGGCGGTGGTCTGGGGTCATTGGAGAACTTGGTAAAAGATCTTTTATCCTTTTTGGATCCCAAGGCTTCCGGGTTTGATAATCTATTGAAGAAAATCAAAGATATAGTTGAAAGCAACGAAAGTAGGAGGCTACCGAAGCTTCCCAAGGGGACTCGGGATTTTGCGAAAGAGCAAATGACAATTAGAAAGAAAGCATTTTCAATTATAGAGAGCGTTTTTGAGCGCCATGGTGCCACAACCTTGGATACTCCAGCTTTTGAGTTGAGAGAAACTCTCACAGGGAAATATGGAGAAGATTCCAAGCTGATTTTTGATCTGGCCGATCAGGGTGGGGAGCTCTATTCGCTTAGATACGACTTGACTGTTCCATTTGCTAGGTATGTGGCCATGAATGGTTTAACATCATTCAAAAGGTATCAAATAGCTAAAGTTTATAGAAGGGACAACCCATCTAAAGGAAGATACCGTGAATTTTATCAATGTGATTTGGATATTGCTGGTCACTATGAAAAGATGGGGCCAGATTTTGAGGTTATCAAGATACTAACAGAGCTACTTGATGAACTGAACATTGGGGAATACGAGATAAAATTGAATCACCGAAAGCTATTGGATGGAATGTTAGAAATTTGTGGAGTACCACCTGAAAAGTTTAGAACCATATGTTCTAGTATTGACAAGTTAGACAAGCAATCGTTTGACCAGATAAAAAAAGAAATGGTGGAGGAAAAGGGGTTGACCGTGGAGGTAGCTGAAAGAATTGGTAATTTTGTGAAGGAAAGGGGACATCCATTAGACTTATTATCTAAGCTTAAACAGGAGCAAAGCGCGTTACTACAAAATAAAGGATCTTCTGATGCATTGAGTGAATTAGATATTTTGTTCAGTGCTCTAGAAAAGTCAAAGTGTATTGATAAAGTGGTATTTGACTTGAGTCTTGCCAGAGGACTTGATTACTACACTGGAGTAATTTATGAAGCTATTTTTAAAGGTGGCACCCAGGTTGGATCTATTGCTGCTGGTGGACGCTTTGACAATCTGATTGGAATGTTTGGCTCCAAGCAGATCCCAGCAGTTGGTATCAGTCTTGGTATTGAGCGAGTTCTTGTTATTATGGAACAAATTTTGAAAGATCAAAACAAGGCTGTTCGGGCAACTAAGACTGATGTTTTAGTGAGCATACTGGGGGATGATTTGACACTAGCAGCAGAGCTTGCAAGTGAGATGTGGGGTGCGAAACTTAACGTTGAATTCCTGGTCAATAAAAGAGTGATGAAACATATCGATCGTGCGAAAGAGTCGAGAATCCCTTGGATAGTATTTTTAGGGGAACGTGAAGTGAGTGAAGGGATTGTGAAGTTGAAAAACGTGGAAACCTTTGAAGAAGTAACAATTTCCAGAAGCAACATCATTGACGAACTCAAGAAACGCCTCACTCCTTGA

Protein sequence

MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHLLIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEELSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKEEVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKGGSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSNESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDVSTNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNKAVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP*
Homology
BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match: F4IYF8 (Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760 PE=2 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 6.3e-285
Identity = 536/888 (60.36%), Postives = 659/888 (74.21%), Query Frame = 0

Query: 6   EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHLL-IPD 65
           E S ITLGGKGSSLSSSSVY +A G A VRIDSSA++R S+     N  SIK     IP 
Sbjct: 4   ERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGIPQ 63

Query: 66  FLTREEARASLVVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTNE 125
            LT EE RASL VLLNKLI+S     SSS  RSV+P+ I E LN K E+L+   +DVT  
Sbjct: 64  GLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTEG 123

Query: 126 ELSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASK 185
           E  V ++SC  L G+C+++DH+ST LS I D+VA +SCE +KAD+++FSL+DSGDG   K
Sbjct: 124 ENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGDK 183

Query: 186 EEVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGK 245
           + +GVA D+KVLLNG K  G++E E ISKIP +HG  R   K VH+  R ELNS VK GK
Sbjct: 184 DVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGGK 243

Query: 246 GGSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPS 305
            GS ++G  +   T LL     + +LG CS  R KL      DEN+K  L+  +   C  
Sbjct: 244 TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLSEKI---CVE 303

Query: 306 NESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQD 365
           NE+LK  YKL     L+E Y  F H +N  L  VW+I   EA AA   + GGEL  +   
Sbjct: 304 NENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKSG 363

Query: 366 VSTNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGN-----GGGLGSLENLVKDLL 425
            +  E ++   KK + +KKAV+GKGTS+V+Q IKDRL  N     G  + SL    + +L
Sbjct: 364 DADKEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQIL 423

Query: 426 SFLDPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFER 485
           +  +P+   FD+LL K+K+IVESNE+RRLPKLPKGTRDFAKEQM +R+KAFSII++VF+R
Sbjct: 424 NLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVFKR 483

Query: 486 HGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTS 545
           HGAT LDTP FELRETL GKYGEDSKL++D+ADQGGEL SLRYDLTVPFARYVAMNG+TS
Sbjct: 484 HGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGITS 543

Query: 546 FKRYQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYE 605
           FKRYQIAKVYRRDNPSKGRYREFYQCD DIAG +E MGPDFE++KILTELLDEL IG+YE
Sbjct: 544 FKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDYE 603

Query: 606 IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIG 665
           +KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+KKEMVEEKGL+ E+A+RIG
Sbjct: 604 VKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIG 663

Query: 666 NFVKERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLAR 725
           NFVKE+G PL+LLSKL+QE S  L N+ S +AL EL I+F AL++SKC +++VFDLSLAR
Sbjct: 664 NFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSLAR 723

Query: 726 GLDYYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQI 785
           GLDYYTGVI+EA+   G +VGSI AGGR+DNLIGMFG+KQ+PAVG+SLGIERV  IME++
Sbjct: 724 GLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIMEEL 783

Query: 786 LKDQNKAVRATKTDVLVSILGDD-LTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKES 845
            + Q + +R T+T VLVSI+ D+ L  AAEL S++WGAK+N E+LV+KR  KH +RAKES
Sbjct: 784 NEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKES 843

Query: 846 RIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 878
            IPW+V +GE+E+S   V LK +    E  E+ T +R   ++ELKK L
Sbjct: 844 GIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883

BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match: P93422 (Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150900 PE=2 SV=2)

HSP 1 Score: 743.8 bits (1919), Expect = 2.2e-213
Identity = 410/760 (53.95%), Postives = 533/760 (70.13%), Query Frame = 0

Query: 138 LDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKEEVGVANDMKVLLNGSKL 197
           +D  ++ L  +ADAVA +SCEA++ DV+AF +  SGDG ++K+E  VA D+K+LL GSKL
Sbjct: 36  IDCCASPLVRVADAVAALSCEAARGDVAAFDVPTSGDGLSAKDEADVAADVKMLLFGSKL 95

Query: 198 VGR---IESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKGGSLSS--GTEDTTR 257
           VG     ++ + +K+P V+G  RE  + +H+R+R+ELN+ VK+GK  ++ +  G E+   
Sbjct: 96  VGAAGGADAASFTKVPTVNGIFREAVRALHARVRIELNAPVKLGKRDAVQTGEGKEEALV 155

Query: 258 TALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSNESLKKEYKLVCE 317
                 A  +  + K S+ R +L +    D  ++  L      +    + LK    ++ +
Sbjct: 156 ALATQLARPVQAMLKLSVARARLCVARIDDAELRKKLT-----DGVEIDDLK---GMLGK 215

Query: 318 LSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDVSTNEA------- 377
           +++D      +  V   L     I +WEA  A+ +IE    I K Q    NEA       
Sbjct: 216 VTIDSDAVSVLRGVYNSLLKFRDILAWEAAVAMAVIEMDSSIEKPQACEENEAGSSTENP 275

Query: 378 ---NEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLV-----KDLLSFL 437
               EK     + KKK  LGKGTS V+ L++D L  NG  + S+ + +      +L    
Sbjct: 276 HASGEKPKGDKKSKKKKTLGKGTSAVLMLLRD-LVTNGKEVLSVNSALLAEWGTELSLLF 335

Query: 438 DPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGA 497
           DPK     +L+ K+K+IVE+NE RRLPK+PKGTRDF KEQM IR+ AFSII  VF+ HGA
Sbjct: 336 DPKCPRLVSLVDKVKEIVETNEVRRLPKIPKGTRDFGKEQMAIREHAFSIITGVFKMHGA 395

Query: 498 TTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKR 557
            +LDTP FELRETL GKYGEDSKLI+DLADQGGEL SLRYDLTVPFARYVAMN ++S KR
Sbjct: 396 VSLDTPVFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNNISSLKR 455

Query: 558 YQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKL 617
           YQIAKVYRRDNPSKGRYREFYQCD DIAG YE M PDFEVIK+LTELLD+L+IG YEIKL
Sbjct: 456 YQIAKVYRRDNPSKGRYREFYQCDFDIAGVYETMEPDFEVIKVLTELLDQLDIGTYEIKL 515

Query: 618 NHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFV 677
           NHRKLLDGMLEICGVPPEKFRT+CSSIDKLDKQ+F+Q+KKE+V+EKG++ E A++IG+ V
Sbjct: 516 NHRKLLDGMLEICGVPPEKFRTVCSSIDKLDKQTFEQVKKELVDEKGISNETADKIGDLV 575

Query: 678 KERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLD 737
           K RG PL++L +L++E S  + N GS  AL+EL+ILF AL+K+  I K+VFDLSLARGLD
Sbjct: 576 KTRGPPLEVLLELRKEGSKFMGNAGSVTALNELEILFKALDKANAIGKIVFDLSLARGLD 635

Query: 738 YYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKD 797
           YYTGVIYEA+FKG TQVGSIAAGGR+DNL+GMF  KQ+PAVG+SLGIERV  IMEQ   +
Sbjct: 636 YYTGVIYEAVFKGTTQVGSIAAGGRYDNLVGMFSGKQVPAVGVSLGIERVFAIMEQQEME 695

Query: 798 QNKAVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPW 857
           +N+ +RAT+T+VLVSI+G DL LAAEL SE+W A +  EF +  R+  H+  A +S IPW
Sbjct: 696 KNQ-IRATETEVLVSIIGKDLILAAELVSELWNAGIKAEFKLTTRIQNHLKYATQSGIPW 755

Query: 858 IVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRL 878
           +V +GE E+S G VKLKN+   +E  + R+     LK++L
Sbjct: 756 MVLVGESEISSGKVKLKNLAASQEEEVDRTEFAQVLKQKL 785

BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match: E9QI36 (Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2)

HSP 1 Score: 461.8 bits (1187), Expect = 1.7e-128
Identity = 244/457 (53.39%), Postives = 328/457 (71.77%), Query Frame = 0

Query: 445 KLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFD 504
           K  KGTRD+  +QM IR+K F+II + F+RHGA T+D+P FEL+ETLTGKYGEDSKLI+D
Sbjct: 57  KTAKGTRDYNPKQMAIREKVFNIIINCFKRHGAETIDSPVFELKETLTGKYGEDSKLIYD 116

Query: 505 LADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQIAKVYRRDNP--SKGRYREFYQCDL 564
           L DQGGEL SLRYDLTVPFARY+AMN +T+ KRY IAKVYRRDNP  ++GRYREFYQCD 
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKITNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176

Query: 565 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICS 624
           DIAG Y+ M PD E +K++ E+L EL++G++ IK+N R++LDGM  ICGVP EKFRTICS
Sbjct: 177 DIAGQYDAMIPDAECLKLVYEILSELDLGDFRIKVNDRRILDGMFAICGVPDEKFRTICS 236

Query: 625 SIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKG 684
           ++DKLDK +++++KKEMV EKGL+ EVA+RI ++V  +G   DL  +L Q+   L Q+K 
Sbjct: 237 TVDKLDKLAWEEVKKEMVNEKGLSEEVADRIRDYVSMQGGK-DLAERLLQDPK-LSQSKQ 296

Query: 685 SSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIF-------------- 744
           +   ++++ +LFS LE  +  DKVVFDLSLARGLDYYTGVIYEAI               
Sbjct: 297 ACAGITDMKLLFSYLELFQITDKVVFDLSLARGLDYYTGVIYEAILTQANPAPASTPAEQ 356

Query: 745 ----KGGTQVGSIAAGGRFDNLIGMFGSK--QIPAVGISLGIERVLVIMEQILKDQNKAV 804
                 G  VGS+A GGR+D L+GMF  K  ++P VG+S+GIERV  IMEQ  +  ++ V
Sbjct: 357 NGAEDAGVSVGSVAGGGRYDGLVGMFDPKGRKVPCVGVSIGIERVFSIMEQKAELSSEKV 416

Query: 805 RATKTDVLV-SILGDDLTLAAELASEMWGAKLNVEFLV--NKRVMKHIDRAKESRIPWIV 864
           R T+T VLV S   + L    +L +E+W A +  E L   N +++  +   +++ IP + 
Sbjct: 417 RTTETQVLVASAQKNLLEERLKLTAELWNAGIKAEVLYKKNPKLLSQLQHCEDTGIPLVA 476

Query: 865 FLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKR 877
            LGE+E+ +G+VKL+NV + EEV + R+ ++DE+KKR
Sbjct: 477 ILGEQELKDGVVKLRNVASREEVDVPRAELVDEVKKR 511

BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match: Q2KI84 (Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 7.1e-127
Identity = 238/447 (53.24%), Postives = 323/447 (72.26%), Query Frame = 0

Query: 445 KLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFD 504
           K PKGTRD++  QM +R+K F +I S F+RHGA  +DTP FEL+ETLTGKYGEDSKLI+D
Sbjct: 57  KTPKGTRDYSPRQMAVREKVFDVIISCFKRHGAEVIDTPVFELKETLTGKYGEDSKLIYD 116

Query: 505 LADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQIAKVYRRDNP--SKGRYREFYQCDL 564
           L DQGGEL SLRYDLTVPFARY+AMN LT+ KRY IAKVYRRDNP  ++GRYREFYQCD 
Sbjct: 117 LKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDF 176

Query: 565 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHRKLLDGMLEICGVPPEKFRTICS 624
           DIAG ++ M PD E +KI+ E+L  L IG++ +K+N R++LDGM  ICGVP  KFRTICS
Sbjct: 177 DIAGQFDPMLPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVPDSKFRTICS 236

Query: 625 SIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKG 684
           S+DKLDK S++++K EMV EKGL  EVA+RIG++V++ G  + L+ +L Q+   L QNK 
Sbjct: 237 SVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHG-GVSLVEQLLQDPK-LSQNKQ 296

Query: 685 SSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIF-----KGGTQ---V 744
           + + L +L +LF  L      DK+ FDLSLARGLDYYTGVIYEA+      + G +   V
Sbjct: 297 ALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQPPARAGEEPLGV 356

Query: 745 GSIAAGGRFDNLIGMFGSK--QIPAVGISLGIERVLVIMEQILKDQNKAVRATKTDVLV- 804
           GS+AAGGR+D L+GMF  K  ++P VG+S+G+ER+  I+EQ L+   + VR T+T VLV 
Sbjct: 357 GSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKVRTTETQVLVA 416

Query: 805 SILGDDLTLAAELASEMWGAKLNVEFLV--NKRVMKHIDRAKESRIPWIVFLGEREVSEG 864
           S     L    +L SE+W A +  E L   N +++  +   +E+ IP +  +GE+E+ +G
Sbjct: 417 SAQKKLLEERLKLISELWDAGIKAELLYKKNPKLLNQLQYCEETGIPLVAIIGEQELKDG 476

Query: 865 IVKLKNVETFEEVTISRSNIIDELKKR 877
           ++KL++V + EEV + R ++++E+K+R
Sbjct: 477 VIKLRSVASREEVDVRREDLVEEIKRR 501

BLAST of Chy3G050100 vs. ExPASy Swiss-Prot
Match: Q86AS6 (Histidine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=hisS PE=3 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.0e-125
Identity = 232/452 (51.33%), Postives = 317/452 (70.13%), Query Frame = 0

Query: 432 KDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETL 491
           K+  E   ++   K PKGT+D+   QMTIR++ F  I+ VF+RHGA T++TP FEL+ETL
Sbjct: 27  KEKKEKKSNKVQLKTPKGTQDYNPRQMTIREQVFDGIKQVFKRHGAVTIETPVFELKETL 86

Query: 492 TGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQIAKVYRRDNP-- 551
           TGKYGEDSKLI+DL DQGGE+ SLRYDLTVPFARYVAMNG+ + KRY IA+VYRRDNP  
Sbjct: 87  TGKYGEDSKLIYDLQDQGGEICSLRYDLTVPFARYVAMNGVLNIKRYHIARVYRRDNPIM 146

Query: 552 SKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHRKLLDGMLEI 611
           +KGR+REFYQCD DIAG Y+ M PD E + ++ E+L+++ +G+++IKLNHRKLLD +  I
Sbjct: 147 TKGRFREFYQCDFDIAGTYDLMVPDAECLVMICEILEQVKVGDFQIKLNHRKLLDAIFAI 206

Query: 612 CGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSK 671
           CGVP +KFR ICS++DKLDK  +++++KEMVE K L   VA++I  FV  +  P+ LL  
Sbjct: 207 CGVPADKFRAICSAVDKLDKSPWEEVRKEMVEVKALDGAVADKIEKFVSLKDEPIKLLQH 266

Query: 672 LKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFK 731
           L+        NK +++ALS+L+ LF  LE       ++FDLSLARGLDYYTG+IYEA+  
Sbjct: 267 LR-ATGMCDGNKDATEALSQLETLFGYLECFGVTQHILFDLSLARGLDYYTGIIYEAVLT 326

Query: 732 GGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNKAVRATKTDV 791
           G  +VGSIAAGGR+D L+GM+G K +PAVG S+GIER+  I+E   K +NK +R   T V
Sbjct: 327 GQDRVGSIAAGGRYDGLVGMYGKKDVPAVGFSIGIERIFTILEDEYKKENKKIRENATQV 386

Query: 792 LVSILGDDLTL-AAELASEMWGAKLNVEF--LVNKRVMKHIDRAKESRIPWIVFLGEREV 851
            V  +  DL      + SE+W A +N EF   VN ++   ++ A ES IP I+ +G+ EV
Sbjct: 387 FVVQMEKDLIKERLAIVSELWKAGINAEFSYKVNPKLPAQLNTADESNIPLIIIIGKSEV 446

Query: 852 SEGIVKLKNVETFEEVTISRSNIIDELKKRLT 879
               + +K +   ++V+I RSN   ++K+ L+
Sbjct: 447 ETNSLSVKTMHDRKQVSIERSNFTVKIKEILS 477

BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match: A0A0A0LSU1 (Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_2G401360 PE=3 SV=1)

HSP 1 Score: 1651.3 bits (4275), Expect = 0.0e+00
Identity = 867/879 (98.63%), Postives = 873/879 (99.32%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL
Sbjct: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
           LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLI ETLNSKPETLQFESLDVTNEE
Sbjct: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLITETLNSKPETLQFESLDVTNEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESL+KEYKLVCELSLDEKYDEFVHTVNVLL TVWKIFSWEATAALL IEGGELIGKGQDV
Sbjct: 301 ESLRKEYKLVCELSLDEKYDEFVHTVNVLLVTVWKIFSWEATAALLTIEGGELIGKGQDV 360

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGTSVVVQ IKD+LQGNGGGLGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVVQSIKDKLQGNGGGLGSLENLVKDLLSFLDPK 420

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRK+AFSIIESVFERHGATTL
Sbjct: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKQAFSIIESVFERHGATTL 480

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIGNFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGNFVKER 660

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
           AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
           LGEREVSEGIVKLKNVETFEEVTISRS IIDELKKRLTP
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSTIIDELKKRLTP 879

BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match: A0A5A7T4J4 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G001410 PE=3 SV=1)

HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN  NAASIKHHL
Sbjct: 1   MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 60

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
            IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 61  FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 360

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 420

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 660

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
            VRATKTDVLVSILGDDLTLAAELASEMW  KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
           LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 879

BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match: A0A5D3C5F8 (Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00730 PE=3 SV=1)

HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN  NAASIKHHL
Sbjct: 60  MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 119

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
            IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 120 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 179

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 180 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 239

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 240 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 299

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 300 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 359

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 360 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 419

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 420 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 479

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 480 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 539

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 540 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 599

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 600 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 659

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 660 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 719

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 720 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 779

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 780 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 839

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
            VRATKTDVLVSILGDDLTLAAELASEMW  KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 840 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 899

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
           LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 900 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 938

BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match: A0A1S3B3G8 (Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103485391 PE=3 SV=1)

HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN  NAASIKHHL
Sbjct: 1   MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 60

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
            IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 61  FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 360

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 420

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 660

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
            VRATKTDVLVSILGDDLTLAAELASEMW  KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
           LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 879

BLAST of Chy3G050100 vs. ExPASy TrEMBL
Match: A0A6J1DGB6 (Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1)

HSP 1 Score: 1502.3 bits (3888), Expect = 0.0e+00
Identity = 785/884 (88.80%), Postives = 829/884 (93.78%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADP+EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSAL+RLSSSSN+ NAAS KH +
Sbjct: 1   MADPSEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALERLSSSSNSRNAASTKHQV 60

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
            IP+FLTREEARASL VLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVT+EE
Sbjct: 61  FIPNFLTREEARASLAVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQS ALSSIADAVA ISCEAS+ADVSAF LMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAAISCEASRADVSAFGLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IE  A SKIPK HGCLREQAKLVHS+MRVELNS +K GKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIEIGAFSKIPKNHGCLREQAKLVHSKMRVELNSCMKFGKG 240

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTED+ RTALLSFAA+LWDLGKCSL RGKLIL S+ +EN+KASL  LL RECPSN
Sbjct: 241 GSLSSGTEDSVRTALLSFAAVLWDLGKCSLYRGKLILSSNSNENVKASLVGLLDRECPSN 300

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESLKKEYKLV EL+LD++YDEFVHTVNVLL TVWKI +WEA AA L IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVSELALDDQYDEFVHTVNVLLVTVWKIVAWEAIAAFLTIEGGELLGKGQDV 360

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNG-----GGLGSLENLVKDLLS 420
             NE NEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQG G     GGLGSLEN VKDLLS
Sbjct: 361 DINEVNEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGKGGGASAGGLGSLENSVKDLLS 420

Query: 421 FLDPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERH 480
           FLDPKAS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERH
Sbjct: 421 FLDPKASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERH 480

Query: 481 GATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSF 540
           GAT LDTPAFELRETL GKYGEDSKLI+DLADQGGEL SLRYDLTVPFARYVAMNG+TSF
Sbjct: 481 GATALDTPAFELRETLMGKYGEDSKLIYDLADQGGELCSLRYDLTVPFARYVAMNGITSF 540

Query: 541 KRYQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEI 600
           KRYQIAKVYRRDNPSKGR+REFYQCD DIAG YEKMGPDFEVIKILTELLDEL+IG+YEI
Sbjct: 541 KRYQIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELSIGQYEI 600

Query: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGN 660
           KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVE+AE+IG+
Sbjct: 601 KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAEKIGD 660

Query: 661 FVKERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARG 720
           FVKERGHPL+LLSKLKQEQ  LLQNKGS DAL EL+ILF+ALEKSKC+DKVVFDLSLARG
Sbjct: 661 FVKERGHPLELLSKLKQEQCPLLQNKGSFDALGELEILFNALEKSKCVDKVVFDLSLARG 720

Query: 721 LDYYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQIL 780
           LDYYTGVIYEA+FKGGTQVGSIAAGGR+DNLIGMFGSKQ+PAVG+SLGIERV VIMEQ+L
Sbjct: 721 LDYYTGVIYEAVFKGGTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLL 780

Query: 781 KDQNKAVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRI 840
           KDQN+  RATKT+VLVSILGDDLT AAELASE+W AKLN EFLVNKRVMKHIDRAKESRI
Sbjct: 781 KDQNQTTRATKTEVLVSILGDDLTQAAELASEIWSAKLNAEFLVNKRVMKHIDRAKESRI 840

Query: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 880
           PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRL+P
Sbjct: 841 PWIVFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLSP 884

BLAST of Chy3G050100 vs. NCBI nr
Match: XP_004138662.1 (histidine--tRNA ligase, cytoplasmic [Cucumis sativus] >KGN63076.1 hypothetical protein Csa_021991 [Cucumis sativus])

HSP 1 Score: 1657 bits (4290), Expect = 0.0
Identity = 867/879 (98.63%), Postives = 873/879 (99.32%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL
Sbjct: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
           LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLI ETLNSKPETLQFESLDVTNEE
Sbjct: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLITETLNSKPETLQFESLDVTNEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNGVCALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGVCALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESL+KEYKLVCELSLDEKYDEFVHTVNVLL TVWKIFSWEATAALL IEGGELIGKGQDV
Sbjct: 301 ESLRKEYKLVCELSLDEKYDEFVHTVNVLLVTVWKIFSWEATAALLTIEGGELIGKGQDV 360

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGTSVVVQ IKD+LQGNGGGLGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVVQSIKDKLQGNGGGLGSLENLVKDLLSFLDPK 420

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRK+AFSIIESVFERHGATTL
Sbjct: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKQAFSIIESVFERHGATTL 480

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIGNFVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGNFVKER 660

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
           AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
           LGEREVSEGIVKLKNVETFEEVTISRS IIDELKKRLTP
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSTIIDELKKRLTP 879

BLAST of Chy3G050100 vs. NCBI nr
Match: TYK06605.1 (histidine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN  NAASIKHHL
Sbjct: 60  MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 119

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
            IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 120 FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 179

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 180 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 239

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 240 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 299

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 300 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 359

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 360 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 419

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 420 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 479

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 480 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 539

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 540 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 599

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 600 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 659

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 660 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 719

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 720 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 779

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 780 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 839

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
            VRATKTDVLVSILGDDLTLAAELASEMW  KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 840 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 899

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
           LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 900 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 938

BLAST of Chy3G050100 vs. NCBI nr
Match: XP_008441178.2 (PREDICTED: histidine--tRNA ligase, cytoplasmic [Cucumis melo] >KAA0037046.1 histidine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 849/879 (96.59%), Postives = 861/879 (97.95%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADP EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSN  NAASIKHHL
Sbjct: 1   MADPIEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNISNAASIKHHL 60

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
            IPDFLT EEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETL+FESLDVTNEE
Sbjct: 61  FIPDFLTPEEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLRFESLDVTNEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQSTALSS+ADAVAGISCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSTALSSVADAVAGISCEASKADVSAFSLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IES AISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESGAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN
Sbjct: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESLKKEYKLVCELSLDEKYDEFVH+VNVLL TVWKIFSWEATAALL IEGGEL+GKGQDV
Sbjct: 301 ESLKKEYKLVCELSLDEKYDEFVHSVNVLLVTVWKIFSWEATAALLTIEGGELLGKGQDV 360

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGTSVV+QLIKDRLQGNGG LGSLENLVKDLLSFLDPK
Sbjct: 361 GTNEANEKVVKKNEKKKKAVLGKGTSVVIQLIKDRLQGNGGDLGSLENLVKDLLSFLDPK 420

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASEFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCD DIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVEVAERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEVAERIGDFVKER 660

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK
Sbjct: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
            VRATKTDVLVSILGDDLTLAAELASEMW  KLNVEFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TVRATKTDVLVSILGDDLTLAAELASEMWSEKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
           LGEREVSEGIVKLKNVETFEEVTISRS+II+ELKKRL P
Sbjct: 841 LGEREVSEGIVKLKNVETFEEVTISRSDIIEELKKRLAP 879

BLAST of Chy3G050100 vs. NCBI nr
Match: XP_038884703.1 (histidine--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida])

HSP 1 Score: 1573 bits (4074), Expect = 0.0
Identity = 816/879 (92.83%), Postives = 851/879 (96.81%), Query Frame = 0

Query: 1   MADPTEVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHL 60
           MADPTEVSVITLGGKGSSLSS+SVYAIAHGFALVRIDSSALDRLSSSSN+ NAAS+KHH+
Sbjct: 1   MADPTEVSVITLGGKGSSLSSTSVYAIAHGFALVRIDSSALDRLSSSSNDQNAASLKHHV 60

Query: 61  LIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNEE 120
            IPDFLTREEARA+LVVLLNKLIIS+SSGIRSVIPVLIA+ LNS+PET +FESLDVT+EE
Sbjct: 61  FIPDFLTREEARAALVVLLNKLIISASSGIRSVIPVLIADKLNSRPETFRFESLDVTDEE 120

Query: 121 LSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASKE 180
           LSVFKQSCYVLNG+CALLDHQS ALSSIADAVAG+SCEASKADVSAFSLMDSGDGFASKE
Sbjct: 121 LSVFKQSCYVLNGICALLDHQSAALSSIADAVAGMSCEASKADVSAFSLMDSGDGFASKE 180

Query: 181 EVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGKG 240
           EVGVANDMKVLLNGSKLVG+IESEAISKIPKVHGCLREQAKLVHSR+RVELNSSVKIGKG
Sbjct: 181 EVGVANDMKVLLNGSKLVGKIESEAISKIPKVHGCLREQAKLVHSRVRVELNSSVKIGKG 240

Query: 241 GSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPSN 300
           GSLSSGTEDT RTALLSFAAMLWDLGKCSL+RGKLILGSSGD+NIKASLA LL RECPSN
Sbjct: 241 GSLSSGTEDTIRTALLSFAAMLWDLGKCSLERGKLILGSSGDDNIKASLAGLLDRECPSN 300

Query: 301 ESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQDV 360
           ESLKKEY+LVCELSLDEK+DEFVHTVNVLL TVWKIF+WEATAALL IEGGEL+ KGQDV
Sbjct: 301 ESLKKEYRLVCELSLDEKHDEFVHTVNVLLVTVWKIFAWEATAALLTIEGGELMVKGQDV 360

Query: 361 STNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLDPK 420
            TNEANEKVVKKNEKKKKAVLGKGT+VVVQLIKDRLQG GGGLGSLENLVKDLLSFLDPK
Sbjct: 361 DTNEANEKVVKKNEKKKKAVLGKGTNVVVQLIKDRLQGKGGGLGSLENLVKDLLSFLDPK 420

Query: 421 ASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGATTL 480
           AS FDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGATTL
Sbjct: 421 ASQFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGATTL 480

Query: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRYQI 540
           DTPAFELRETLTGKYGEDSKLIFDLADQGGEL SLRYDLTVPFARYVAMNGLTSFKRYQI
Sbjct: 481 DTPAFELRETLTGKYGEDSKLIFDLADQGGELCSLRYDLTVPFARYVAMNGLTSFKRYQI 540

Query: 541 AKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLNHR 600
           AKVYRRDNPSKGRYREFYQCD DIAG YEKMGPDFEVIKI+TELLDELNIG+YEIKLNHR
Sbjct: 541 AKVYRRDNPSKGRYREFYQCDFDIAGQYEKMGPDFEVIKIMTELLDELNIGQYEIKLNHR 600

Query: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKER 660
           KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVE AERIG+FVKER
Sbjct: 601 KLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVETAERIGDFVKER 660

Query: 661 GHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYT 720
           GHPLDLLSKLKQE+S LLQNKGSSDALS+L+ILFSALEKSKCIDKVVFDLSLARGLDYYT
Sbjct: 661 GHPLDLLSKLKQEESVLLQNKGSSDALSDLEILFSALEKSKCIDKVVFDLSLARGLDYYT 720

Query: 721 GVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNK 780
           GVIYEA+FKGGTQVGSIAAGGRFDNLIGMFGSKQ+PAVGISLGIERVLVIMEQ LKDQ +
Sbjct: 721 GVIYEAVFKGGTQVGSIAAGGRFDNLIGMFGSKQVPAVGISLGIERVLVIMEQNLKDQKQ 780

Query: 781 AVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWIVF 840
             RATKTD+LVSILGDDLTLAAELASEMW AKL+ EFLVNKRVMKHIDRAKESRIPWIVF
Sbjct: 781 TARATKTDILVSILGDDLTLAAELASEMWSAKLHAEFLVNKRVMKHIDRAKESRIPWIVF 840

Query: 841 LGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
           LGEREVSEGIVKLKNVETFEE+TI RSNIIDELKKRLTP
Sbjct: 841 LGEREVSEGIVKLKNVETFEEITIPRSNIIDELKKRLTP 879

BLAST of Chy3G050100 vs. NCBI nr
Match: XP_023550553.1 (histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1509 bits (3907), Expect = 0.0
Identity = 779/881 (88.42%), Postives = 830/881 (94.21%), Query Frame = 0

Query: 1   MADPT-EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHH 60
           MADP  E+SVITLGGKGSSL+S+SVYAIAHGFALVRIDSS+L+R+SSSSNNPNAASIKHH
Sbjct: 1   MADPAAELSVITLGGKGSSLASTSVYAIAHGFALVRIDSSSLERISSSSNNPNAASIKHH 60

Query: 61  LLIPDFLTREEARASLVVLLNKLIISSSSGIRSVIPVLIAETLNSKPETLQFESLDVTNE 120
           L +PDFLTREEARASLVVLLNKLIIS+SSGIRSVIP+LIAETLNS PETLQFE LDVT E
Sbjct: 61  LFVPDFLTREEARASLVVLLNKLIISTSSGIRSVIPILIAETLNSNPETLQFEPLDVTPE 120

Query: 121 ELSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASK 180
           ELSVF+QSCY+LNG+CALLDHQS ALSSIADAVA ISCEAS+ADVSAFSLMDSGDGFASK
Sbjct: 121 ELSVFEQSCYILNGICALLDHQSAALSSIADAVASISCEASRADVSAFSLMDSGDGFASK 180

Query: 181 EEVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGK 240
           EEVG+ANDMKV LNGSKLVG+IES AISKIPK HGCLREQAKLVHS+MRVELNSSVKIGK
Sbjct: 181 EEVGIANDMKVFLNGSKLVGKIESGAISKIPKTHGCLREQAKLVHSKMRVELNSSVKIGK 240

Query: 241 GG-SLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECP 300
           GG SLSSGTED  RT LLSFAAMLWDLGKCSLDR KLI GS+GDEN+KASLA LL RECP
Sbjct: 241 GGGSLSSGTEDAVRTVLLSFAAMLWDLGKCSLDRVKLIWGSNGDENMKASLAGLLDRECP 300

Query: 301 SNESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQ 360
           SNESLK+EY+LV EL LDEKY EFVH VNVL  TVWKI +WEA AA + IEGGELIGKGQ
Sbjct: 301 SNESLKREYRLVSELGLDEKYVEFVHAVNVLFATVWKIVAWEAIAAFMTIEGGELIGKGQ 360

Query: 361 DVSTNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGNGGGLGSLENLVKDLLSFLD 420
           DV  N ANEKVVKKNEKKKKAVLGKG+SVVVQLIKD+LQG GGGLGSLENLVKDLLSFLD
Sbjct: 361 DVDINGANEKVVKKNEKKKKAVLGKGSSVVVQLIKDKLQGKGGGLGSLENLVKDLLSFLD 420

Query: 421 PKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFERHGAT 480
           PKASGFDNLLKKIK+IVESNESRRLPKLPKGTRDFAKEQM IRKKAFSIIE VFERHGAT
Sbjct: 421 PKASGFDNLLKKIKEIVESNESRRLPKLPKGTRDFAKEQMAIRKKAFSIIEGVFERHGAT 480

Query: 481 TLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTSFKRY 540
            LDTPAFELRETL GKYGEDSKL++DLADQGGEL SLRYDLTVPFARYVAMNG+TSFKRY
Sbjct: 481 ALDTPAFELRETLMGKYGEDSKLVYDLADQGGELCSLRYDLTVPFARYVAMNGITSFKRY 540

Query: 541 QIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYEIKLN 600
           QIAKVYRRDNPSKGR+REFYQCD DIAG YEKMGPDFEVIKILTELLDELNIG YEIKLN
Sbjct: 541 QIAKVYRRDNPSKGRFREFYQCDFDIAGQYEKMGPDFEVIKILTELLDELNIGHYEIKLN 600

Query: 601 HRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVK 660
           HR+LLDGMLEICGVP +KFRTICSSIDKLDKQSFDQIK+EMVEEKGLTVE+AERIG+FVK
Sbjct: 601 HRRLLDGMLEICGVPSDKFRTICSSIDKLDKQSFDQIKREMVEEKGLTVEIAERIGDFVK 660

Query: 661 ERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDY 720
           ERGHPL+LLSKLKQEQS LLQNKGSSDAL+EL+ILFSALEKS C+DKVVFDLSLARGLDY
Sbjct: 661 ERGHPLELLSKLKQEQSPLLQNKGSSDALNELEILFSALEKSNCVDKVVFDLSLARGLDY 720

Query: 721 YTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQ 780
           YTGVIYEA+FKGGTQ+GSIAAGGR+DNLIGMFGSKQ+PAVG+SLGIERV VIMEQ+LKDQ
Sbjct: 721 YTGVIYEAVFKGGTQIGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFVIMEQLLKDQ 780

Query: 781 NKAVRATKTDVLVSILGDDLTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKESRIPWI 840
           N+  RATKT+VLVSILGDDLT AAELASE+W AK++ EFLVNKRVMKHIDRAKE+RIPWI
Sbjct: 781 NQTTRATKTEVLVSILGDDLTQAAELASEIWSAKVHAEFLVNKRVMKHIDRAKEARIPWI 840

Query: 841 VFLGEREVSEGIVKLKNVETFEEVTISRSNIIDELKKRLTP 879
           VFLGERE SEGIVKLKNVETFEEVT+SRSNIIDELKKRLTP
Sbjct: 841 VFLGEREASEGIVKLKNVETFEEVTVSRSNIIDELKKRLTP 881

BLAST of Chy3G050100 vs. TAIR 10
Match: AT3G02760.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 981.5 bits (2536), Expect = 4.5e-286
Identity = 536/888 (60.36%), Postives = 659/888 (74.21%), Query Frame = 0

Query: 6   EVSVITLGGKGSSLSSSSVYAIAHGFALVRIDSSALDRLSSSSNNPNAASIKHHLL-IPD 65
           E S ITLGGKGSSLSSSSVY +A G A VRIDSSA++R S+     N  SIK     IP 
Sbjct: 4   ERSSITLGGKGSSLSSSSVYNVASGVANVRIDSSAIERFSTR----NVPSIKRSSFGIPQ 63

Query: 66  FLTREEARASLVVLLNKLIIS-----SSSGIRSVIPVLIAETLNSKPETLQFESLDVTNE 125
            LT EE RASL VLLNKLI+S     SSS  RSV+P+ I E LN K E+L+   +DVT  
Sbjct: 64  GLTNEETRASLAVLLNKLILSTSGPPSSSTARSVLPLKIVEILNLKAESLELGEIDVTEG 123

Query: 126 ELSVFKQSCYVLNGVCALLDHQSTALSSIADAVAGISCEASKADVSAFSLMDSGDGFASK 185
           E  V ++SC  L G+C+++DH+ST LS I D+VA +SCE +KAD+++FSL+DSGDG   K
Sbjct: 124 ENIVLEKSCASLIGICSIIDHKSTTLSQIVDSVAALSCEVTKADIASFSLLDSGDGNGDK 183

Query: 186 EEVGVANDMKVLLNGSKLVGRIESEAISKIPKVHGCLREQAKLVHSRMRVELNSSVKIGK 245
           + +GVA D+KVLLNG K  G++E E ISKIP +HG  R   K VH+  R ELNS VK GK
Sbjct: 184 DVIGVAGDLKVLLNGYKGTGKLEIEEISKIPWIHGKFRYVVKSVHADARRELNSGVKGGK 243

Query: 246 GGSLSSGTEDTTRTALLSFAAMLWDLGKCSLDRGKLILGSSGDENIKASLASLLARECPS 305
            GS ++G  +   T LL     + +LG CS  R KL      DEN+K  L+  +   C  
Sbjct: 244 TGSGNTGIGEALGTTLLPLLTAIKNLGVCSFLRAKLCFEKIVDENLKKCLSEKI---CVE 303

Query: 306 NESLKKEYKLVCELSLDEKYDEFVHTVNVLLETVWKIFSWEATAALLIIEGGELIGKGQD 365
           NE+LK  YKL     L+E Y  F H +N  L  VW+I   EA AA   + GGEL  +   
Sbjct: 304 NENLKNSYKLAYTAHLEEDYCRFAHKLNECLGIVWRIVGLEAVAAFFALAGGELFVQKSG 363

Query: 366 VSTNEANEKVVKKNEKKKKAVLGKGTSVVVQLIKDRLQGN-----GGGLGSLENLVKDLL 425
            +  E ++   KK + +KKAV+GKGTS+V+Q IKDRL  N     G  + SL    + +L
Sbjct: 364 DADKEESKTDKKKKKNEKKAVVGKGTSLVIQFIKDRLVSNDAASDGDQMHSLMQCGEQIL 423

Query: 426 SFLDPKASGFDNLLKKIKDIVESNESRRLPKLPKGTRDFAKEQMTIRKKAFSIIESVFER 485
           +  +P+   FD+LL K+K+IVESNE+RRLPKLPKGTRDFAKEQM +R+KAFSII++VF+R
Sbjct: 424 NLFNPEGRSFDSLLDKVKEIVESNENRRLPKLPKGTRDFAKEQMAVREKAFSIIQNVFKR 483

Query: 486 HGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDLTVPFARYVAMNGLTS 545
           HGAT LDTP FELRETL GKYGEDSKL++D+ADQGGEL SLRYDLTVPFARYVAMNG+TS
Sbjct: 484 HGATALDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGITS 543

Query: 546 FKRYQIAKVYRRDNPSKGRYREFYQCDLDIAGHYEKMGPDFEVIKILTELLDELNIGEYE 605
           FKRYQIAKVYRRDNPSKGRYREFYQCD DIAG +E MGPDFE++KILTELLDEL IG+YE
Sbjct: 544 FKRYQIAKVYRRDNPSKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDYE 603

Query: 606 IKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIG 665
           +KLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSF+Q+KKEMVEEKGL+ E+A+RIG
Sbjct: 604 VKLNHRKLLDGMLEICGVPPEKFRTICSSIDKLDKQSFEQVKKEMVEEKGLSSEIADRIG 663

Query: 666 NFVKERGHPLDLLSKLKQEQSALLQNKGSSDALSELDILFSALEKSKCIDKVVFDLSLAR 725
           NFVKE+G PL+LLSKL+QE S  L N+ S +AL EL I+F AL++SKC +++VFDLSLAR
Sbjct: 664 NFVKEKGAPLELLSKLRQEGSEFLDNQSSREALDELSIMFEALKRSKCSERIVFDLSLAR 723

Query: 726 GLDYYTGVIYEAIFKGGTQVGSIAAGGRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQI 785
           GLDYYTGVI+EA+   G +VGSI AGGR+DNLIGMFG+KQ+PAVG+SLGIERV  IME++
Sbjct: 724 GLDYYTGVIFEAVCI-GAEVGSIGAGGRYDNLIGMFGTKQVPAVGMSLGIERVFNIMEEL 783

Query: 786 LKDQNKAVRATKTDVLVSILGDD-LTLAAELASEMWGAKLNVEFLVNKRVMKHIDRAKES 845
            + Q + +R T+T VLVSI+ D+ L  AAEL S++WGAK+N E+LV+KR  KH +RAKES
Sbjct: 784 NEKQKQVIRPTETQVLVSIMVDNKLAEAAELVSQLWGAKINAEYLVSKRKEKHFNRAKES 843

Query: 846 RIPWIVFLGEREVSEGIVKLKNV----ETFEEVTISRSNIIDELKKRL 878
            IPW+V +GE+E+S   V LK +    E  E+ T +R   ++ELKK L
Sbjct: 844 GIPWMVMVGEKELSGSFVTLKKLEKGSEEKEDQTCTRDRFVEELKKLL 883

BLAST of Chy3G050100 vs. TAIR 10
Match: AT3G46100.1 (Histidyl-tRNA synthetase 1 )

HSP 1 Score: 157.9 bits (398), Expect = 3.7e-38
Identity = 122/432 (28.24%), Postives = 218/432 (50.46%), Query Frame = 0

Query: 447 PKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSK-LIFDL 506
           PKGTRDF  E M +R   F+  + V   +G   +D P  E       K GE+ +  ++  
Sbjct: 72  PKGTRDFPPEDMRLRNWLFNHFKEVSRLYGYEEVDYPVLETEALFIRKAGEEIRDQLYCF 131

Query: 507 ADQGGELYSLRYDLTVPFARYVAMNGLT---SFKRYQIAKVYRRDNPSKGRYREFYQCDL 566
            D+G    +LR +LT   AR V   G +     K + I + +R +  ++GR RE YQ ++
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAIGQCWRYERMTRGRRREHYQWNM 191

Query: 567 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEI--KLNHRKLLDGMLEICGVPPEKFRTI 626
           DI G   ++  + E+I  +      + I   ++  K++ RK+L  +L+  GVP + F  +
Sbjct: 192 DIIG-VPQVTAEAELISSIVTFFKRIGITASDVGFKVSSRKVLQELLKKYGVPEDLFGRV 251

Query: 627 CSSIDKLDKQSFDQIKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQN 686
           C  IDK++K   D+IKKE+    G++ +  E++   +  +   LD L  +          
Sbjct: 252 CIIIDKIEKIPIDEIKKEL-GFTGISEDAIEQLLQVLSVKS--LDDLEDI---------I 311

Query: 687 KGSSDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVGSIAAG 746
            G+ +A+++L  LFS  EK    + + FD S+ RGL YYTG+++E   + G ++ +I  G
Sbjct: 312 GGAGEAIADLKQLFSLAEKFGYSEWIQFDASVVRGLAYYTGIVFEGFDRKG-KLRAICGG 371

Query: 747 GRFDNLIGMFGSKQIPAVGISLGIERVLVIMEQILKDQNKAVR-ATKTDVLVSILGDDLT 806
           GR+D L+  +G    PA G   G + V+V   ++LK+++       + + +V  L  DL 
Sbjct: 372 GRYDRLLSTYGGDDFPACGFGFG-DAVIV---ELLKEKDLLPELGQEVENIVCALDKDLQ 431

Query: 807 -LAAELASEMWGAKLNVEFLVNKRVMKHI-DRAKESRIPWIVFLGEREVSEGIVKLKNVE 866
             AA +A+ +      V+ ++  + +K +  RA       +V +G+ E  +G V +K + 
Sbjct: 432 GAAATVATALRDKGQTVDLVLESKPLKWVFKRAARVNARRLVLVGKTEWEDGSVSVKVLS 485

Query: 867 TFEEVTISRSNI 870
           + E+  +  S++
Sbjct: 492 SGEQFQVKLSDL 485

BLAST of Chy3G050100 vs. TAIR 10
Match: AT3G59410.1 (protein kinase family protein )

HSP 1 Score: 107.5 bits (267), Expect = 5.7e-23
Identity = 110/445 (24.72%), Postives = 196/445 (44.04%), Query Frame = 0

Query: 460  IRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDL 519
            +R     I + VF +H A  L+     +   L     + S+    L   GG++  L Y+L
Sbjct: 796  LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 855

Query: 520  TVPFARYVAMNGLTSFKRYQIAKVYRR------DNPSKGRYREFYQCDLDIAGHYEKMGP 579
             +PF  ++++N  +SFKRY+I+ VYRR       NP         Q D DI G    +  
Sbjct: 856  RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 915

Query: 580  DFEVIKILTELLDEL-NIGEYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 639
            + EV+K++ ++   + + G  +I LNH  LLD +    G+  E   K   + S +  L  
Sbjct: 916  EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 975

Query: 640  QSFDQ------IKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKGS 699
            QS ++      I++++++E  L   V  R+           D    L + + AL  ++ +
Sbjct: 976  QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDAD--QALPRLRGALRADRPT 1035

Query: 700  SDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVGS------I 759
              AL EL  L + L   +  + V  D+ +     Y+  + ++         G+      +
Sbjct: 1036 RKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLL 1095

Query: 760  AAGGRFDNLIGMFGSKQ----IP-AVGISLGIERVLVIMEQILKDQNKAVRATKTDVLVS 819
            A GGR+D L+     ++    +P AVG+SL +E    I + +  D         T VLV 
Sbjct: 1096 AVGGRYDWLVQEVCDREHKMNLPGAVGVSLALE---TIFQHLPMDLRPIRNEVSTSVLVC 1155

Query: 820  ILGDD--LTLAAELASEMWGAKLNVEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVSE 871
              G    L    EL +E+W   +  EF+   +  + +  + A E  I  +V + E  V++
Sbjct: 1156 SRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQ 1215

BLAST of Chy3G050100 vs. TAIR 10
Match: AT3G59410.2 (protein kinase family protein )

HSP 1 Score: 107.5 bits (267), Expect = 5.7e-23
Identity = 110/445 (24.72%), Postives = 196/445 (44.04%), Query Frame = 0

Query: 460  IRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSKLIFDLADQGGELYSLRYDL 519
            +R     I + VF +H A  L+     +   L     + S+    L   GG++  L Y+L
Sbjct: 820  LRDYVVEITKEVFRQHCAKHLEV----IPMRLLSDCPQFSRKTVKLLTNGGDMLELCYEL 879

Query: 520  TVPFARYVAMNGLTSFKRYQIAKVYRR------DNPSKGRYREFYQCDLDIAGHYEKMGP 579
             +PF  ++++N  +SFKRY+I+ VYRR       NP         Q D DI G    +  
Sbjct: 880  RLPFVHWISVNQKSSFKRYEISHVYRRAIGHSPPNPC-------LQADFDIVGGTLSL-T 939

Query: 580  DFEVIKILTELLDEL-NIGEYEIKLNHRKLLDGMLEICGVPPE---KFRTICSSIDKLDK 639
            + EV+K++ ++   + + G  +I LNH  LLD +    G+  E   K   + S +  L  
Sbjct: 940  EAEVLKVIVDITTHIFHRGSCDIHLNHGDLLDAIWSWAGIKAEHRRKVAELLSMMGSLRP 999

Query: 640  QSFDQ------IKKEMVEEKGLTVEVAERIGNFVKERGHPLDLLSKLKQEQSALLQNKGS 699
            QS ++      I++++++E  L   V  R+           D    L + + AL  ++ +
Sbjct: 1000 QSSERKLKWVFIRRQLLQELKLPEAVVNRLQTVASRFCGDAD--QALPRLRGALRADRPT 1059

Query: 700  SDALSELDILFSALEKSKCIDKVVFDLSLARGLDYYTGVIYEAIFKGGTQVGS------I 759
              AL EL  L + L   +  + V  D+ +     Y+  + ++         G+      +
Sbjct: 1060 RKALDELSNLLTYLRVWRIEEHVHIDVLMPPTESYHRNLFFQVFLTKENSSGTSNDGVLL 1119

Query: 760  AAGGRFDNLIGMFGSKQ----IP-AVGISLGIERVLVIMEQILKDQNKAVRATKTDVLVS 819
            A GGR+D L+     ++    +P AVG+SL +E    I + +  D         T VLV 
Sbjct: 1120 AVGGRYDWLVQEVCDREHKMNLPGAVGVSLALE---TIFQHLPMDLRPIRNEVSTSVLVC 1179

Query: 820  ILGDD--LTLAAELASEMWGAKLNVEFL--VNKRVMKHIDRAKESRIPWIVFLGEREVSE 871
              G    L    EL +E+W   +  EF+   +  + +  + A E  I  +V + E  V++
Sbjct: 1180 SRGGGGLLVQRMELVAELWEKSIKAEFVPTPDPSLTEQYEYANEHEIKCLVIITESGVAQ 1239

BLAST of Chy3G050100 vs. TAIR 10
Match: AT5G03406.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 2.3e-16
Identity = 56/187 (29.95%), Postives = 92/187 (49.20%), Query Frame = 0

Query: 447 PKGTRDFAKEQMTIRKKAFSIIESVFERHGATTLDTPAFELRETLTGKYGEDSK-LIFDL 506
           PKGTRDF  E M +R   F+  + V    G   +D P  E       K GE+ +  ++  
Sbjct: 72  PKGTRDFPPEDMRLRNWLFNHFKEVSRLFGYEEVDFPVLETEALFIRKAGEEIRDQLYCF 131

Query: 507 ADQGGELYSLRYDLTVPFARYVAMNGLT---SFKRYQIAKVYRRDNPSKGRYREFYQCDL 566
            D+G    +LR +LT   AR V   G +     K + + + +R +  ++GR RE YQ ++
Sbjct: 132 EDRGNRRVALRPELTPSLARLVIQKGKSVSLPLKWFAVGQCWRYERMTRGRRREHYQWNM 191

Query: 567 DIAGHYEKMGPDFEVIKILTELLDELNIGEYEI--KLNHRKLLDGMLEICGVPPEKFRTI 626
           DI G   ++  + E+I  +      + I   ++  K++ RK+L   L+  GVP + F  +
Sbjct: 192 DIIG-VPEVTAEAELISSIMTFFKRIGITASDVGFKVSSRKVLQEFLKKYGVPEDLFGRV 251

Query: 627 CSSIDKL 628
           C  IDK+
Sbjct: 252 CFIIDKV 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IYF86.3e-28560.36Histidine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g02760... [more]
P934222.2e-21353.95Histidine--tRNA ligase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
E9QI361.7e-12853.39Histidine--tRNA ligase OS=Danio rerio OX=7955 GN=hars PE=3 SV=2[more]
Q2KI847.1e-12753.24Histidine--tRNA ligase, cytoplasmic OS=Bos taurus OX=9913 GN=HARS1 PE=2 SV=1[more]
Q86AS61.0e-12551.33Histidine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=hisS... [more]
Match NameE-valueIdentityDescription
A0A0A0LSU10.0e+0098.63Histidine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_2G401360 PE=3 SV=1[more]
A0A5A7T4J40.0e+0096.59Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A5D3C5F80.0e+0096.59Histidine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... [more]
A0A1S3B3G80.0e+0096.59Histidine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103485391 PE=3 SV=1[more]
A0A6J1DGB60.0e+0088.80Histidine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111020184 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004138662.10.098.63histidine--tRNA ligase, cytoplasmic [Cucumis sativus] >KGN63076.1 hypothetical p... [more]
TYK06605.10.096.59histidine--tRNA ligase [Cucumis melo var. makuwa][more]
XP_008441178.20.096.59PREDICTED: histidine--tRNA ligase, cytoplasmic [Cucumis melo] >KAA0037046.1 hist... [more]
XP_038884703.10.092.83histidine--tRNA ligase, cytoplasmic isoform X1 [Benincasa hispida][more]
XP_023550553.10.088.42histidine--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G02760.14.5e-28660.36Class II aaRS and biotin synthetases superfamily protein [more]
AT3G46100.13.7e-3828.24Histidyl-tRNA synthetase 1 [more]
AT3G59410.15.7e-2324.72protein kinase family protein [more]
AT3G59410.25.7e-2324.72protein kinase family protein [more]
AT5G03406.12.3e-1629.95Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 129..236
e-value: 1.5E-6
score: 29.4
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 433..782
e-value: 4.1E-140
score: 468.7
NoneNo IPR availablePANTHERPTHR11476HISTIDYL-TRNA SYNTHETASEcoord: 131..878
NoneNo IPR availablePANTHERPTHR11476:SF7HISTIDYL-TRNA SYNTHETASEcoord: 131..878
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 787..877
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 445..771
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 791..875
e-value: 1.4E-12
score: 47.6
IPR015807Histidine-tRNA ligaseTIGRFAMTIGR00442TIGR00442coord: 446..865
e-value: 1.7E-97
score: 324.9
IPR015807Histidine-tRNA ligaseHAMAPMF_00127His_tRNA_synthcoord: 442..875
score: 23.694609
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 783..878
e-value: 2.5E-19
score: 70.9
IPR041715Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domainPFAMPF13393tRNA-synt_Hiscoord: 449..767
e-value: 1.3E-44
score: 152.7
IPR041715Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core domainCDDcd00773HisRS-like_corecoord: 457..772
e-value: 7.41932E-100
score: 309.918
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 425..771
score: 10.771797
IPR033656Histidyl-anticodon-bindingCDDcd00859HisRS_anticodoncoord: 787..874
e-value: 1.63601E-19
score: 81.8172
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 15..261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G050100.1Chy3G050100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006427 histidyl-tRNA aminoacylation
biological_process GO:0032543 mitochondrial translation
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004821 histidine-tRNA ligase activity
molecular_function GO:0003824 catalytic activity