Chy2G049230 (gene) Cucumber (hystrix) v1

Overview
NameChy2G049230
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioncytochrome P450 94B3
LocationchrH02: 29595310 .. 29597326 (-)
RNA-Seq ExpressionChy2G049230
SyntenyChy2G049230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCGGCTCGGTTCGAGGCGGACCATTCTGACGGCTAACCCAGCCAATGTAGAACACATGTTGAAGACTAATTTCCTTAATTATCCTAAAGGGAAGCCGTTTACTGATATACTTGGCGATCTACTTGGGTGTGGCATTTTTAACGTCGATGGCGATTTATGGTCCACACAAAGGAAGCTTGCCAGTCATGCATTTTCCGCTAAGTCGTTAAGGGAATTCGTGGTTAAAACGCTTGAAGATGAAGTTCATTTTCGTTTAATTCCGTTGCTTCATAACGCTGCGAGAACTAACGCTGTTCTTGATTTGCAAGATGTTTTGGGAAGACTAGCTTTTGACACCGTTTGTAAGGTTTGTGATCGATCTCTCCATGATCATATATTTATCTTCTTGCATGCATGACTTCAATTTTTGTATGTATTATATATAATATTGTATTCAACTAAAACACAATACAGTACTGCTACCCTTAAAAGTACTTCAGCTTTTCTGTTTAATTTGTTTCCAAAATAACAAAAAATACTCATATATTTAATAAAATTGCAATATTATTGTTTCAATATGACCCTTGGAAGAACAATTCTTTAAAACCTTGAGTATAAAACTGTGTTGCAAAAACCTAAAACAATTTGATGACCTAATTTTTTTTATTATAGAAGTATTTTTTAAACAGATTGTAAGGTTTGAGAACATTTTTACATAATTTAATCAATTTTTAAAATTATGATAAATTTATCACATGGAACCATATGGTTTGGACCATAAGAAATGTGATTGCAGCTATGTATTACCTATAATGAAAAGAAAAACACTTTGACAGTACATGCTGAGTTGCATTATTTCTCCTTGAAAAGCTGAAAAAGTTGTTGAATTAAAAATTGTCACATGACAAGGGAACTTTTTATAAGAATTGATCGTTACTTCCATGTTAGTTATTGGAGAGATATGTATGATGATCTAGATTTGCACCTAAAATTCTACCAAACTTACTAATCGAGAAGTTCACCAATTTGGATAGGTTGATTGATTTTGTTTGTTGTTTACATTTTCTCAGGTAACGTTGGGAACGGACGAGCAATGTTTAGACATGTCGCGCCCAGTCCCAGAAATTGTAAAAGCATTCGACGTGGCAACGGCGATCAGCGCAAGGCGGGCAGTGACACCACTCTACCTCACGTGGAAGGTCAAGCGCATGCTCAACCTCGGCTCTGAAAAGAAGCTAAAACAAGTTGTTCAAACGGTTCACGAATGGATCTCCAATATCATTCACAACAAAGTCCTCAATAACAACGATAATAAAAATCATAATCATAACCAAACACACAACAACAGCGATCTGTTATCTCGTCTCTTATCGGCCGGCCTCAACGAGGAAGTCATAAGAGACATGATCGTAAGCTTCATAATGGCTGGCCGCGACACCACCTCAGCTGCCATGACATGGCTCTTTTGGTTGCTCACAAACCACCTGAACATCGAACAAACCATAATCAACGAAGCCACTTTATTGTCCAATCACGATTCGAAAACCACGTCGTTGGGTTACGGTTACGAAGAGTTGAAAGATATGAAGTATTTGAAAGCGTGTTTATGCGAGTCAATGAGGCTATACCCACCAGTAGCATGGGATTCAAAGCATGCAGCAGCCGCGGACATTCTCCCGGACGGAACTCAAGTGAGAAAAGGGGATAGAGTGACGTATTTTCCATACGGAATGGGACGGATGAAGGAGCTGTGGGGAAAAGACCGGCTAGAGTTCAAACCGGAGAGGTGGTTCCAAAACGGGGAGTTGAAAACGGTGAGTGCGTTCAAGTTTCCGGTGTTTCAAGCGGGACCAAGGATGTGTTTGGGGACGGAAATGGCGTTCATTCAGATGAAATACGTGATGGCCACGGTTTTGAAACGGTTTGAATTTAGACGAGTGAGTGAAAATAATGAGCCGGTTTTTGTCCCGTTGCTGACAGCGCATATGGCGGGTGGACTAAAGGTTTATGTCAGGGAAAGGACGGAACAATAG

mRNA sequence

ATGCATCGGCTCGGTTCGAGGCGGACCATTCTGACGGCTAACCCAGCCAATGTAGAACACATGTTGAAGACTAATTTCCTTAATTATCCTAAAGGGAAGCCGTTTACTGATATACTTGGCGATCTACTTGGGTGTGGCATTTTTAACGTCGATGGCGATTTATGGTCCACACAAAGGAAGCTTGCCAGTCATGCATTTTCCGCTAAGTCGTTAAGGGAATTCGTGGTTAAAACGCTTGAAGATGAAGTTCATTTTCGTTTAATTCCGTTGCTTCATAACGCTGCGAGAACTAACGCTGTTCTTGATTTGCAAGATGTTTTGGGAAGACTAGCTTTTGACACCGTTTGTAAGGTAACGTTGGGAACGGACGAGCAATGTTTAGACATGTCGCGCCCAGTCCCAGAAATTGTAAAAGCATTCGACGTGGCAACGGCGATCAGCGCAAGGCGGGCAGTGACACCACTCTACCTCACGTGGAAGGTCAAGCGCATGCTCAACCTCGGCTCTGAAAAGAAGCTAAAACAAGTTGTTCAAACGGTTCACGAATGGATCTCCAATATCATTCACAACAAAGTCCTCAATAACAACGATAATAAAAATCATAATCATAACCAAACACACAACAACAGCGATCTGTTATCTCGTCTCTTATCGGCCGGCCTCAACGAGGAAGTCATAAGAGACATGATCGTAAGCTTCATAATGGCTGGCCGCGACACCACCTCAGCTGCCATGACATGGCTCTTTTGGTTGCTCACAAACCACCTGAACATCGAACAAACCATAATCAACGAAGCCACTTTATTGTCCAATCACGATTCGAAAACCACGTCGTTGGGTTACGGTTACGAAGAGTTGAAAGATATGAAGTATTTGAAAGCGTGTTTATGCGAGTCAATGAGGCTATACCCACCAGTAGCATGGGATTCAAAGCATGCAGCAGCCGCGGACATTCTCCCGGACGGAACTCAAGTGAGAAAAGGGGATAGAGTGACGTATTTTCCATACGGAATGGGACGGATGAAGGAGCTGTGGGGAAAAGACCGGCTAGAGTTCAAACCGGAGAGGTGGTTCCAAAACGGGGAGTTGAAAACGGTGAGTGCGTTCAAGTTTCCGGTGTTTCAAGCGGGACCAAGGATGTGTTTGGGGACGGAAATGGCGTTCATTCAGATGAAATACGTGATGGCCACGGTTTTGAAACGGTTTGAATTTAGACGAGTGAGTGAAAATAATGAGCCGGTTTTTGTCCCGTTGCTGACAGCGCATATGGCGGGTGGACTAAAGGTTTATGTCAGGGAAAGGACGGAACAATAG

Coding sequence (CDS)

ATGCATCGGCTCGGTTCGAGGCGGACCATTCTGACGGCTAACCCAGCCAATGTAGAACACATGTTGAAGACTAATTTCCTTAATTATCCTAAAGGGAAGCCGTTTACTGATATACTTGGCGATCTACTTGGGTGTGGCATTTTTAACGTCGATGGCGATTTATGGTCCACACAAAGGAAGCTTGCCAGTCATGCATTTTCCGCTAAGTCGTTAAGGGAATTCGTGGTTAAAACGCTTGAAGATGAAGTTCATTTTCGTTTAATTCCGTTGCTTCATAACGCTGCGAGAACTAACGCTGTTCTTGATTTGCAAGATGTTTTGGGAAGACTAGCTTTTGACACCGTTTGTAAGGTAACGTTGGGAACGGACGAGCAATGTTTAGACATGTCGCGCCCAGTCCCAGAAATTGTAAAAGCATTCGACGTGGCAACGGCGATCAGCGCAAGGCGGGCAGTGACACCACTCTACCTCACGTGGAAGGTCAAGCGCATGCTCAACCTCGGCTCTGAAAAGAAGCTAAAACAAGTTGTTCAAACGGTTCACGAATGGATCTCCAATATCATTCACAACAAAGTCCTCAATAACAACGATAATAAAAATCATAATCATAACCAAACACACAACAACAGCGATCTGTTATCTCGTCTCTTATCGGCCGGCCTCAACGAGGAAGTCATAAGAGACATGATCGTAAGCTTCATAATGGCTGGCCGCGACACCACCTCAGCTGCCATGACATGGCTCTTTTGGTTGCTCACAAACCACCTGAACATCGAACAAACCATAATCAACGAAGCCACTTTATTGTCCAATCACGATTCGAAAACCACGTCGTTGGGTTACGGTTACGAAGAGTTGAAAGATATGAAGTATTTGAAAGCGTGTTTATGCGAGTCAATGAGGCTATACCCACCAGTAGCATGGGATTCAAAGCATGCAGCAGCCGCGGACATTCTCCCGGACGGAACTCAAGTGAGAAAAGGGGATAGAGTGACGTATTTTCCATACGGAATGGGACGGATGAAGGAGCTGTGGGGAAAAGACCGGCTAGAGTTCAAACCGGAGAGGTGGTTCCAAAACGGGGAGTTGAAAACGGTGAGTGCGTTCAAGTTTCCGGTGTTTCAAGCGGGACCAAGGATGTGTTTGGGGACGGAAATGGCGTTCATTCAGATGAAATACGTGATGGCCACGGTTTTGAAACGGTTTGAATTTAGACGAGTGAGTGAAAATAATGAGCCGGTTTTTGTCCCGTTGCTGACAGCGCATATGGCGGGTGGACTAAAGGTTTATGTCAGGGAAAGGACGGAACAATAG

Protein sequence

MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTLGTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDTTSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQNGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLLTAHMAGGLKVYVRERTEQ*
Homology
BLAST of Chy2G049230 vs. ExPASy Swiss-Prot
Match: Q9FMV7 (Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.0e-150
Identity = 272/440 (61.82%), Postives = 334/440 (75.91%), Query Frame = 0

Query: 4   LGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLAS 63
           L  RRTI+TANP NVEH+LKTNF N+PKGKPFTD+LGDLLG GIFN DG+LWS+QRKLAS
Sbjct: 78  LFGRRTIITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLAS 137

Query: 64  HAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTLGTD 123
           H F+ +SLREF  + L +EV  RLIP+L +A      +D Q+VL R AFD VCKV+LG D
Sbjct: 138 HEFTMRSLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWD 197

Query: 124 EQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEW 183
             CLD++RPVPE+VKAFDVA  ISARRA  P+Y  WKVKR LN+GSEK+L++ ++TVH  
Sbjct: 198 PDCLDLTRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLS 257

Query: 184 ISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDTTSA 243
           +S II  K  + +   + +  Q     DLLSR L+AG  EE +RD ++SFIMAGRDTTSA
Sbjct: 258 VSEIIRAKKKSLDIGGDVSDKQ-----DLLSRFLAAGHGEEAVRDSVISFIMAGRDTTSA 317

Query: 244 AMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLY 303
           AMTWLFWLL+ + ++E  I++E   L N      SLG G+E+L++M Y KACLCE+MRLY
Sbjct: 318 AMTWLFWLLSQNDDVETKILDE---LRNKG----SLGLGFEDLREMSYTKACLCEAMRLY 377

Query: 304 PPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQNGE- 363
           PPVAWDSKHAA  DILPDGT ++KGD+VTYFPYGMGRM+++WGKD  EFKP RWF+    
Sbjct: 378 PPVAWDSKHAANDDILPDGTPLKKGDKVTYFPYGMGRMEKVWGKDWDEFKPNRWFEEEPS 437

Query: 364 ------LKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFV 423
                 LK+VS+FKFPVFQAGPR+C+G EMAF QMKYV+ +VL RF+   V  NN PVFV
Sbjct: 438 YGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQMKYVVGSVLSRFKIIPVC-NNRPVFV 497

Query: 424 PLLTAHMAGGLKVYVRERTE 437
           PLLTAHMAGGLKV ++ R +
Sbjct: 498 PLLTAHMAGGLKVKIKRREQ 504

BLAST of Chy2G049230 vs. ExPASy Swiss-Prot
Match: Q9SMP5 (Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 5.6e-149
Identity = 264/438 (60.27%), Postives = 327/438 (74.66%), Query Frame = 0

Query: 4   LGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLAS 63
           LG+RRTI+T NP NVE++LKTNF N+PKGKPFTD+LGDLLG GIFNVDG  WS+QRKLAS
Sbjct: 76  LGNRRTIITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNVDGHSWSSQRKLAS 135

Query: 64  HAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTLGTD 123
           H FS +SLR F  + L+DEV  RL+P+L  AA     +DLQDVL R AFD VCKV+LG D
Sbjct: 136 HEFSTRSLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRFAFDVVCKVSLGWD 195

Query: 124 EQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEW 183
             CLD++RPV  +V+AFD A  ISARRA  P+Y  WK KR+LN+GSE+KL++ ++TVH  
Sbjct: 196 PDCLDLTRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVGSERKLREAIRTVHVL 255

Query: 184 ISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDTTSA 243
           +S I+  K  +          Q     DLLSR L+AG N E +RDM++SFIMAGRDTTSA
Sbjct: 256 VSEIVRAKKKSLEIGTGAEAKQ-----DLLSRFLAAGHNGEAVRDMVISFIMAGRDTTSA 315

Query: 244 AMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLY 303
           AMTWLFWLLT + ++E+ I+ E   L        SLG G+E+LK+M Y KACLCE+MRLY
Sbjct: 316 AMTWLFWLLTENDDVERKILEEVDPL-------VSLGLGFEDLKEMAYTKACLCEAMRLY 375

Query: 304 PPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQNGE- 363
           PPV+WDSKHAA  D+LPDGT+V++GD+VTYFPYGMGRM+ LWG D  EF P RWF +   
Sbjct: 376 PPVSWDSKHAANDDVLPDGTRVKRGDKVTYFPYGMGRMETLWGTDSEEFNPNRWFDSEPG 435

Query: 364 -----LKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVP 423
                LK +S +KFPVFQAGPR+C+G EMAF+QMKYV+ +VL RFE   V++ + PVFVP
Sbjct: 436 STRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIVPVNK-DRPVFVP 495

Query: 424 LLTAHMAGGLKVYVRERT 436
           LLTAHMAGGLKV ++ R+
Sbjct: 496 LLTAHMAGGLKVKIKRRS 500

BLAST of Chy2G049230 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.3e-100
Identity = 201/435 (46.21%), Postives = 278/435 (63.91%), Query Frame = 0

Query: 9   TILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHAFSA 68
           +++TANP+NVEH+LKTNF NYPKGK F+ ILGDLLG GIFN DGD W  QRKLAS    +
Sbjct: 75  SVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIFNSDGDTWRFQRKLASLELGS 134

Query: 69  KSLREFVVKTLEDEVHFRLIPLLHN-AARTNAVLDLQDVLGRLAFDTVCKVTLGTDEQCL 128
            S+R F  + ++ E+  RL+P+L + +    +VLDLQDV  R +FDT+ K++ G D  CL
Sbjct: 135 VSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVFRRFSFDTISKLSFGFDPDCL 194

Query: 129 DMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEWISNI 188
            +  P+ E   AFD A+ +SA+RA+ P  L WK KR+L +GSEKKL++ +  ++    ++
Sbjct: 195 RLPFPISEFAVAFDTASLLSAKRALAPFPLLWKTKRLLRIGSEKKLQESINVINRLAGDL 254

Query: 189 IHNKVLNNNDNKNHNHNQTHNNSDLLSRLLS--AGLNEEVIRDMIVSFIMAGRDTTSAAM 248
           I  + L     KN          DL+SR ++  A  ++E +RD++VSF++AGRDT +A +
Sbjct: 255 IKQRRLTGLMGKN----------DLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGL 314

Query: 249 TWLFWLLTNHLNIEQTIINE--ATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLY 308
           T  FWLLT H  +E  I  E    + +  DS T       +E+++M YL A L ESMRL+
Sbjct: 315 TGFFWLLTRHPEVENRIREELDRVMGTGFDSVTARC----DEMREMDYLHASLYESMRLF 374

Query: 309 PPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQN-GE 368
           PPV +DSK A   D+L DGT V  G RVTY  Y MGRM  +WG D  EFKPERW  N G+
Sbjct: 375 PPVQFDSKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEGK 434

Query: 369 LKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVS-ENNEPV-FVPLLT 428
            +  +  K+PVFQAG R+C+G EMA ++MK +   +++RFE R  S E  E + F P LT
Sbjct: 435 FRPENPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASPETTETLRFAPGLT 494

Query: 429 AHMAGGLKVYVRERT 436
           A + GGL V ++ER+
Sbjct: 495 ATVNGGLPVMIQERS 495

BLAST of Chy2G049230 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 361.7 bits (927), Expect = 1.2e-98
Identity = 191/436 (43.81%), Postives = 272/436 (62.39%), Query Frame = 0

Query: 7   RRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHAF 66
           +R I+T NP+ V+H+LK  F NY KG  FT+ L D LG GIFN +G  W  QR++ASH F
Sbjct: 91  KRQIITGNPSTVQHILKNQFSNYQKGTTFTNTLSDFLGTGIFNTNGPNWKFQRQVASHEF 150

Query: 67  SAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTLGTDEQC 126
           + KS+R FV   ++ E+  RLIP+L ++ +TN +LD QD+L R  FD +C +  G D + 
Sbjct: 151 NTKSIRNFVEHIVDTELTNRLIPILTSSTQTNNILDFQDILQRFTFDNICNIAFGYDPEY 210

Query: 127 LDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEWISN 186
           L  S    +  +A++ AT IS++R   PL + WK+K+  N+GSEK+LK+ V  V  +   
Sbjct: 211 LTPSTNRSKFAEAYEDATEISSKRFRLPLPIIWKIKKYFNIGSEKRLKEAVTEVRSFAKK 270

Query: 187 IIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAG-LNEEVIRDMIVSFIMAGRDTTSAAM 246
           ++  K       +      +    D+LSR LS+G  +E+ + D+++SFI+AG+DTTSAA+
Sbjct: 271 LVREK------KRELEEKSSLETEDMLSRFLSSGHSDEDFVADIVISFILAGKDTTSAAL 330

Query: 247 TWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLYPP 306
           TW FWLL  +  +E+ I+NE        SK + L   Y+E+K+M Y  A L ESMRLYPP
Sbjct: 331 TWFFWLLWKNPRVEEEIVNEL-------SKKSEL-MVYDEVKEMVYTHAALSESMRLYPP 390

Query: 307 VAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQNGEL-- 366
           V  DSK A   D+LPDG  V+KG  VTY  Y MGRMK LWG D  EF+PERW +  E+  
Sbjct: 391 VPMDSKEAVNDDVLPDGWVVKKGTIVTYHVYAMGRMKSLWGDDWAEFRPERWLEKDEVNG 450

Query: 367 KTV----SAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFE-FRRVSENNEPVFVPL 426
           K V     ++ +PVFQAGPR+CLG EMAF+QMK ++A ++ +F+         EP F+  
Sbjct: 451 KWVFVGRDSYSYPVFQAGPRVCLGKEMAFMQMKRIVAGIVGKFKVVPEAHLAQEPGFISF 510

Query: 427 LTAHMAGGLKVYVRER 435
           L++ M GG  V +++R
Sbjct: 511 LSSQMEGGFPVTIQKR 512

BLAST of Chy2G049230 vs. ExPASy Swiss-Prot
Match: P98188 (Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 7.6e-98
Identity = 194/442 (43.89%), Postives = 271/442 (61.31%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           +HR    R + TA PA V+H+L+TNF  Y KG  F   + D LG GIFN DG+ W  QR+
Sbjct: 80  LHRPFGARQVFTAQPAVVQHILRTNFTCYGKGLTFYQSINDFLGDGIFNADGESWKFQRQ 139

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           ++SH F+ +SLR+FV   ++ E+  RL+P+L  A+ +   LD QD+L RL FD +C +  
Sbjct: 140 ISSHEFNTRSLRKFVETVVDVELSDRLVPVLSQASNSQTTLDFQDILQRLTFDNICMIAF 199

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           G D + L  S P     KAFD ++ +S  R    + L WKVKR LN+G E++LK+ V  V
Sbjct: 200 GYDPEYLLPSLPEIPFAKAFDESSQLSIERLNALIPLLWKVKRFLNIGVERQLKEAVAEV 259

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEE-VIRDMIVSFIMAGRD 240
               + I+ NK     + K         + DLLSR LS+G ++E  + DM++S I+AGRD
Sbjct: 260 RGLATKIVKNK---KKELKEKALQSESESVDLLSRFLSSGHSDESFVTDMVISIILAGRD 319

Query: 241 TTSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCES 300
           TTSAA+TW FWLL+ H ++E  I+ E T       K+ ++  GY+E+KDM Y  A LCES
Sbjct: 320 TTSAALTWFFWLLSKHSHVENEILKEIT------GKSETV--GYDEVKDMVYTHAALCES 379

Query: 301 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 360
           MRLYPP+  D+K A   D+LPDGT V+KG RVTY  Y MGR +++WG D  EF+PERW  
Sbjct: 380 MRLYPPLPVDTKVAVHDDVLPDGTLVKKGWRVTYHIYAMGRSEKIWGPDWAEFRPERWLS 439

Query: 361 NGELKT-----VSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFE-FRRVSENNEP 420
             E+       +  + +PVFQAGPR+C+G EMAF+QMK V+A ++ RF     + E  EP
Sbjct: 440 RDEVGKWSFVGIDYYSYPVFQAGPRVCIGKEMAFLQMKRVVAGIMGRFRVVPAMVEGIEP 499

Query: 421 VFVPLLTAHMAGGLKVYVRERT 436
            +    T+ M GG  V + +R+
Sbjct: 500 EYTAHFTSVMKGGFPVKIEKRS 510

BLAST of Chy2G049230 vs. ExPASy TrEMBL
Match: A0A0A0LR92 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002090 PE=4 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.0e-246
Identity = 429/438 (97.95%), Postives = 433/438 (98.86%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 78  MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 137

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL
Sbjct: 138 LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 197

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+PEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV
Sbjct: 198 GTDEQCLDMSRPIPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 257

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 240
           HEWISNIIHNKVLNNNDNK+ NHNQT NNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT
Sbjct: 258 HEWISNIIHNKVLNNNDNKDRNHNQTQNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 317

Query: 241 TSAAMTWLFWLLTNHLNIEQTIINEATLLSNHD-SKTTSLGYGYEELKDMKYLKACLCES 300
           TSAAMTWLFWLLTNH NIEQTIINEAT LS+HD SKTTSLGYGYEELKDMKYLKACLCES
Sbjct: 318 TSAAMTWLFWLLTNHRNIEQTIINEATSLSDHDYSKTTSLGYGYEELKDMKYLKACLCES 377

Query: 301 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 360
           MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRM+ELWGKDRLEFKPERWFQ
Sbjct: 378 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMEELWGKDRLEFKPERWFQ 437

Query: 361 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 420
           NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL
Sbjct: 438 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 497

Query: 421 TAHMAGGLKVYVRERTEQ 438
           TAHMAGGLKVYVRERTEQ
Sbjct: 498 TAHMAGGLKVYVRERTEQ 515

BLAST of Chy2G049230 vs. ExPASy TrEMBL
Match: A0A5D3BH58 (Cytochrome P450 94B3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00590 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.3e-235
Identity = 411/438 (93.84%), Postives = 424/438 (96.80%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           +HRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 73  LHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 132

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAV+DLQDVLGRLAFDTVCKVTL
Sbjct: 133 LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVVDLQDVLGRLAFDTVCKVTL 192

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+PEIV AFDVATAISARRAV PLYLTWK KRMLNLGSEKKLK+VVQTV
Sbjct: 193 GTDEQCLDMSRPIPEIVNAFDVATAISARRAVAPLYLTWKAKRMLNLGSEKKLKEVVQTV 252

Query: 181 HEWISNIIHNKVLNNNDNKN-HNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRD 240
           HEWISNIIH+K+LNNN+N   HNHNQTHNN DLLSRLLSAGL+EEV+RDMIVSFIMAGRD
Sbjct: 253 HEWISNIIHSKLLNNNNNNTVHNHNQTHNN-DLLSRLLSAGLDEEVVRDMIVSFIMAGRD 312

Query: 241 TTSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCES 300
           TTSAAMTWLFWLLTNH NIEQTIINEAT LS+ DSKTTSLGYGYEELKDMKYLKACLCES
Sbjct: 313 TTSAAMTWLFWLLTNHRNIEQTIINEATSLSD-DSKTTSLGYGYEELKDMKYLKACLCES 372

Query: 301 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 360
           MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRM+ELWGKDRLEFKPERWFQ
Sbjct: 373 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMEELWGKDRLEFKPERWFQ 432

Query: 361 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 420
           NGELKTVS FKFPVFQAGPRMCLG EMAFIQMKYVMATVLKRFEFR VSENN PVFVPLL
Sbjct: 433 NGELKTVSPFKFPVFQAGPRMCLGMEMAFIQMKYVMATVLKRFEFRPVSENNVPVFVPLL 492

Query: 421 TAHMAGGLKVYVRERTEQ 438
           TAHMAGGL+V+VRERTEQ
Sbjct: 493 TAHMAGGLQVFVRERTEQ 508

BLAST of Chy2G049230 vs. ExPASy TrEMBL
Match: A0A1S3CN07 (cytochrome P450 94B3 OS=Cucumis melo OX=3656 GN=LOC103502328 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.3e-235
Identity = 411/438 (93.84%), Postives = 424/438 (96.80%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           +HRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 78  LHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 137

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAV+DLQDVLGRLAFDTVCKVTL
Sbjct: 138 LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVVDLQDVLGRLAFDTVCKVTL 197

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+PEIV AFDVATAISARRAV PLYLTWK KRMLNLGSEKKLK+VVQTV
Sbjct: 198 GTDEQCLDMSRPIPEIVNAFDVATAISARRAVAPLYLTWKAKRMLNLGSEKKLKEVVQTV 257

Query: 181 HEWISNIIHNKVLNNNDNKN-HNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRD 240
           HEWISNIIH+K+LNNN+N   HNHNQTHNN DLLSRLLSAGL+EEV+RDMIVSFIMAGRD
Sbjct: 258 HEWISNIIHSKLLNNNNNNTVHNHNQTHNN-DLLSRLLSAGLDEEVVRDMIVSFIMAGRD 317

Query: 241 TTSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCES 300
           TTSAAMTWLFWLLTNH NIEQTIINEAT LS+ DSKTTSLGYGYEELKDMKYLKACLCES
Sbjct: 318 TTSAAMTWLFWLLTNHRNIEQTIINEATSLSD-DSKTTSLGYGYEELKDMKYLKACLCES 377

Query: 301 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 360
           MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRM+ELWGKDRLEFKPERWFQ
Sbjct: 378 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMEELWGKDRLEFKPERWFQ 437

Query: 361 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 420
           NGELKTVS FKFPVFQAGPRMCLG EMAFIQMKYVMATVLKRFEFR VSENN PVFVPLL
Sbjct: 438 NGELKTVSPFKFPVFQAGPRMCLGMEMAFIQMKYVMATVLKRFEFRPVSENNVPVFVPLL 497

Query: 421 TAHMAGGLKVYVRERTEQ 438
           TAHMAGGL+V+VRERTEQ
Sbjct: 498 TAHMAGGLQVFVRERTEQ 513

BLAST of Chy2G049230 vs. ExPASy TrEMBL
Match: A0A6J1E0I0 (cytochrome P450 94B3 OS=Cucurbita moschata OX=3662 GN=LOC111429724 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.8e-209
Identity = 365/437 (83.52%), Postives = 395/437 (90.39%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           ++RLGSRRT++TANPANVEHMLKTNF+NYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 79  VNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 138

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASH FSAKS+REFVVKTLEDEVH RLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVT+
Sbjct: 139 LASHEFSAKSVREFVVKTLEDEVHLRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTV 198

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+P IV + D AT ISARRAV PL++TWK KRMLN+GSEKKLK+ V+ V
Sbjct: 199 GTDEQCLDMSRPIPAIVNSLDTATGISARRAVAPLFVTWKAKRMLNVGSEKKLKEAVEMV 258

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 240
           HEW+SNII N+ LNN++  +HN    H + DLLSRLLSAG ++EVIRDM+VSFIMAGRDT
Sbjct: 259 HEWVSNIIRNRNLNNSNTLDHN----HAHGDLLSRLLSAGHSQEVIRDMVVSFIMAGRDT 318

Query: 241 TSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESM 300
           TSAAMTWLFWLLT H  +EQ I++EA  LS  DS+T SLGYGYEELKDMKYLKACLCESM
Sbjct: 319 TSAAMTWLFWLLTKHPKVEQAIVDEAIKLSG-DSETASLGYGYEELKDMKYLKACLCESM 378

Query: 301 RLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQN 360
           RLYPPVAWDSKHAAA DILPDGT VRKGDRVTYFPYGMGRM+ELWGKDRLEFKP+RWFQN
Sbjct: 379 RLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEFKPDRWFQN 438

Query: 361 GELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLLT 420
           GELK VS FKFPVFQAGPRMCLG EMAFIQMKYVMATVLKRFEFR  +E N PVFVPLLT
Sbjct: 439 GELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANE-NAPVFVPLLT 498

Query: 421 AHMAGGLKVYVRERTEQ 438
           AHMAGGLKVYV ER EQ
Sbjct: 499 AHMAGGLKVYVGERKEQ 509

BLAST of Chy2G049230 vs. ExPASy TrEMBL
Match: A0A6J1JAK9 (cytochrome P450 94B3-like OS=Cucurbita maxima OX=3661 GN=LOC111485059 PE=3 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.5e-208
Identity = 365/437 (83.52%), Postives = 394/437 (90.16%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           ++RLGSRRT++TANPANVEHMLKTNF+NYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 79  VNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 138

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASH FSAKS+REFVVKTLEDEVH RLIPLL NAARTNAVLDLQDVLGRLAFDTVCKVT+
Sbjct: 139 LASHEFSAKSVREFVVKTLEDEVHLRLIPLLQNAARTNAVLDLQDVLGRLAFDTVCKVTV 198

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+P IV + D AT ISA RAV PL+LTWK KRMLN+GSEKKLK+ V+ V
Sbjct: 199 GTDEQCLDMSRPLPAIVNSLDTATGISAGRAVAPLFLTWKAKRMLNVGSEKKLKEAVEMV 258

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 240
           HEW+SNII N+ LNN++  +HNH  TH   DLLSRLLSAG ++EV+RDM+VSFIMAGRDT
Sbjct: 259 HEWVSNIIRNRNLNNSNTLDHNH--TH--GDLLSRLLSAGHSQEVVRDMVVSFIMAGRDT 318

Query: 241 TSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESM 300
           TSAAMTWLFWLLT H  +EQ I++EA  LS  DS+T SLGYGYEELKDMKYLKACLCESM
Sbjct: 319 TSAAMTWLFWLLTKHPKVEQVIVDEAIKLSG-DSETASLGYGYEELKDMKYLKACLCESM 378

Query: 301 RLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQN 360
           RLYPPVAWDSKHAAA DILPDGT VRKGDRVTYFPYGMGRM+ELWGKDRLEFKP+RWFQN
Sbjct: 379 RLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEFKPDRWFQN 438

Query: 361 GELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLLT 420
           GELK VS FKFPVFQAGPRMCLG EMAFIQMKYVMATVLKRFEFR  +E N PVFVPLLT
Sbjct: 439 GELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANE-NAPVFVPLLT 498

Query: 421 AHMAGGLKVYVRERTEQ 438
           AHMAGGLKVYV ER EQ
Sbjct: 499 AHMAGGLKVYVGERKEQ 509

BLAST of Chy2G049230 vs. NCBI nr
Match: XP_011651847.1 (cytochrome P450 94B3 [Cucumis sativus] >KGN63489.1 hypothetical protein Csa_013660 [Cucumis sativus])

HSP 1 Score: 858 bits (2216), Expect = 2.01e-312
Identity = 429/438 (97.95%), Postives = 433/438 (98.86%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 78  MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 137

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL
Sbjct: 138 LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 197

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+PEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV
Sbjct: 198 GTDEQCLDMSRPIPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 257

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 240
           HEWISNIIHNKVLNNNDNK+ NHNQT NNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT
Sbjct: 258 HEWISNIIHNKVLNNNDNKDRNHNQTQNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 317

Query: 241 TSAAMTWLFWLLTNHLNIEQTIINEATLLSNHD-SKTTSLGYGYEELKDMKYLKACLCES 300
           TSAAMTWLFWLLTNH NIEQTIINEAT LS+HD SKTTSLGYGYEELKDMKYLKACLCES
Sbjct: 318 TSAAMTWLFWLLTNHRNIEQTIINEATSLSDHDYSKTTSLGYGYEELKDMKYLKACLCES 377

Query: 301 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 360
           MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRM+ELWGKDRLEFKPERWFQ
Sbjct: 378 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMEELWGKDRLEFKPERWFQ 437

Query: 361 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 420
           NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL
Sbjct: 438 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 497

Query: 421 TAHMAGGLKVYVRERTEQ 437
           TAHMAGGLKVYVRERTEQ
Sbjct: 498 TAHMAGGLKVYVRERTEQ 515

BLAST of Chy2G049230 vs. NCBI nr
Match: KAA0057776.1 (cytochrome P450 94B3 [Cucumis melo var. makuwa] >TYJ98457.1 cytochrome P450 94B3 [Cucumis melo var. makuwa])

HSP 1 Score: 819 bits (2115), Expect = 3.75e-297
Identity = 411/438 (93.84%), Postives = 424/438 (96.80%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           +HRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 73  LHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 132

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAV+DLQDVLGRLAFDTVCKVTL
Sbjct: 133 LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVVDLQDVLGRLAFDTVCKVTL 192

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+PEIV AFDVATAISARRAV PLYLTWK KRMLNLGSEKKLK+VVQTV
Sbjct: 193 GTDEQCLDMSRPIPEIVNAFDVATAISARRAVAPLYLTWKAKRMLNLGSEKKLKEVVQTV 252

Query: 181 HEWISNIIHNKVLNNNDNKN-HNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRD 240
           HEWISNIIH+K+LNNN+N   HNHNQTHNN DLLSRLLSAGL+EEV+RDMIVSFIMAGRD
Sbjct: 253 HEWISNIIHSKLLNNNNNNTVHNHNQTHNN-DLLSRLLSAGLDEEVVRDMIVSFIMAGRD 312

Query: 241 TTSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCES 300
           TTSAAMTWLFWLLTNH NIEQTIINEAT LS+ DSKTTSLGYGYEELKDMKYLKACLCES
Sbjct: 313 TTSAAMTWLFWLLTNHRNIEQTIINEATSLSD-DSKTTSLGYGYEELKDMKYLKACLCES 372

Query: 301 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 360
           MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRM+ELWGKDRLEFKPERWFQ
Sbjct: 373 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMEELWGKDRLEFKPERWFQ 432

Query: 361 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 420
           NGELKTVS FKFPVFQAGPRMCLG EMAFIQMKYVMATVLKRFEFR VSENN PVFVPLL
Sbjct: 433 NGELKTVSPFKFPVFQAGPRMCLGMEMAFIQMKYVMATVLKRFEFRPVSENNVPVFVPLL 492

Query: 421 TAHMAGGLKVYVRERTEQ 437
           TAHMAGGL+V+VRERTEQ
Sbjct: 493 TAHMAGGLQVFVRERTEQ 508

BLAST of Chy2G049230 vs. NCBI nr
Match: XP_008464443.1 (PREDICTED: cytochrome P450 94B3 [Cucumis melo])

HSP 1 Score: 819 bits (2115), Expect = 4.52e-297
Identity = 411/438 (93.84%), Postives = 424/438 (96.80%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           +HRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 78  LHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 137

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAV+DLQDVLGRLAFDTVCKVTL
Sbjct: 138 LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVVDLQDVLGRLAFDTVCKVTL 197

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+PEIV AFDVATAISARRAV PLYLTWK KRMLNLGSEKKLK+VVQTV
Sbjct: 198 GTDEQCLDMSRPIPEIVNAFDVATAISARRAVAPLYLTWKAKRMLNLGSEKKLKEVVQTV 257

Query: 181 HEWISNIIHNKVLNNNDNKN-HNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRD 240
           HEWISNIIH+K+LNNN+N   HNHNQTHNN DLLSRLLSAGL+EEV+RDMIVSFIMAGRD
Sbjct: 258 HEWISNIIHSKLLNNNNNNTVHNHNQTHNN-DLLSRLLSAGLDEEVVRDMIVSFIMAGRD 317

Query: 241 TTSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCES 300
           TTSAAMTWLFWLLTNH NIEQTIINEAT LS+ DSKTTSLGYGYEELKDMKYLKACLCES
Sbjct: 318 TTSAAMTWLFWLLTNHRNIEQTIINEATSLSD-DSKTTSLGYGYEELKDMKYLKACLCES 377

Query: 301 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 360
           MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRM+ELWGKDRLEFKPERWFQ
Sbjct: 378 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMEELWGKDRLEFKPERWFQ 437

Query: 361 NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLL 420
           NGELKTVS FKFPVFQAGPRMCLG EMAFIQMKYVMATVLKRFEFR VSENN PVFVPLL
Sbjct: 438 NGELKTVSPFKFPVFQAGPRMCLGMEMAFIQMKYVMATVLKRFEFRPVSENNVPVFVPLL 497

Query: 421 TAHMAGGLKVYVRERTEQ 437
           TAHMAGGL+V+VRERTEQ
Sbjct: 498 TAHMAGGLQVFVRERTEQ 513

BLAST of Chy2G049230 vs. NCBI nr
Match: XP_038879988.1 (cytochrome P450 94B3-like [Benincasa hispida])

HSP 1 Score: 758 bits (1958), Expect = 4.08e-273
Identity = 379/437 (86.73%), Postives = 404/437 (92.45%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           M RLGSRRTI+TANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWS+QRK
Sbjct: 78  MQRLGSRRTIVTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSSQRK 137

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASHAFSAKSLREFVVKTLEDEVH RLIPLL NAA+TNAVLDLQDVLGRLAFDTVCKVTL
Sbjct: 138 LASHAFSAKSLREFVVKTLEDEVHLRLIPLLQNAAQTNAVLDLQDVLGRLAFDTVCKVTL 197

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRP+PEIV AFD AT +SA+RA+ PL+LTWK KRMLNLGSEKKLK+ VQ V
Sbjct: 198 GTDEQCLDMSRPIPEIVNAFDTATGVSAKRAIAPLFLTWKAKRMLNLGSEKKLKEAVQMV 257

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 240
           H+W+SNII NK LNNN+N   NHNQTHNN DLLSRL SAG +EEV+RDMI+SFIMAGRDT
Sbjct: 258 HKWVSNIIRNKNLNNNNNTLPNHNQTHNN-DLLSRLQSAGHDEEVVRDMIISFIMAGRDT 317

Query: 241 TSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESM 300
           TSAAMTWLFWLLT H  +E+ IINEA+ LS+ D K+TSLGYGYEELKDMKYLKACLCESM
Sbjct: 318 TSAAMTWLFWLLTKHPEVEEAIINEASSLSD-DLKSTSLGYGYEELKDMKYLKACLCESM 377

Query: 301 RLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQN 360
           RLYPPVAWDSKHAAA DILPDGT VRKGDRVTYFPYGMGRM+ELWGKDRLEFKP+RW+QN
Sbjct: 378 RLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEFKPDRWYQN 437

Query: 361 GELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLLT 420
           GELKTVS FKFPVFQAGPRMCLG EMAFIQMKYV+ATVLKRFEFR  SE N PVFVPLLT
Sbjct: 438 GELKTVSPFKFPVFQAGPRMCLGMEMAFIQMKYVVATVLKRFEFRPASE-NVPVFVPLLT 497

Query: 421 AHMAGGLKVYVRERTEQ 437
           AHMAGGL+VYVRERTEQ
Sbjct: 498 AHMAGGLQVYVRERTEQ 511

BLAST of Chy2G049230 vs. NCBI nr
Match: XP_023516482.1 (cytochrome P450 94B3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 738 bits (1906), Expect = 3.01e-265
Identity = 368/437 (84.21%), Postives = 397/437 (90.85%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           ++RLGSRRT++TANPANVEHMLKTNF+NYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK
Sbjct: 79  VNRLGSRRTVVTANPANVEHMLKTNFVNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 138

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LASH FSAKS+REFVVKTLEDEVH RLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVT+
Sbjct: 139 LASHEFSAKSVREFVVKTLEDEVHLRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTV 198

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           GTDEQCLDMSRPVP IV + D AT ISARRAV PL++TWK KRMLN+GSEKKLK+ V+ V
Sbjct: 199 GTDEQCLDMSRPVPAIVNSLDTATGISARRAVAPLFVTWKAKRMLNVGSEKKLKEAVEMV 258

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 240
           HEW+SNIIHN+ LNN++  +HNH  TH   DLLSRLLSAG ++EV+RDM+VSFIMAGRDT
Sbjct: 259 HEWVSNIIHNRNLNNSNTLDHNH--TH--GDLLSRLLSAGHSQEVVRDMVVSFIMAGRDT 318

Query: 241 TSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESM 300
           TSAAMTWLFWLLT H  +EQ I++EA  LS  DS+T SLGYGYEELKDMKYLKACLCESM
Sbjct: 319 TSAAMTWLFWLLTKHPKVEQAIVDEAIKLSG-DSETASLGYGYEELKDMKYLKACLCESM 378

Query: 301 RLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQN 360
           RLYPPVAWDSKHAAA DILPDGT VRKGDRVTYFPYGMGRM+ELWGKDRLEFKP+RWFQN
Sbjct: 379 RLYPPVAWDSKHAAAPDILPDGTPVRKGDRVTYFPYGMGRMEELWGKDRLEFKPDRWFQN 438

Query: 361 GELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVPLLT 420
           GELK VS FKFPVFQAGPRMCLG EMAFIQMKYVMATVLKRFEFR  +E N PVFVPLLT
Sbjct: 439 GELKPVSPFKFPVFQAGPRMCLGKEMAFIQMKYVMATVLKRFEFRPANE-NAPVFVPLLT 498

Query: 421 AHMAGGLKVYVRERTEQ 437
           AHMAGGLKVYV ER EQ
Sbjct: 499 AHMAGGLKVYVGERKEQ 509

BLAST of Chy2G049230 vs. TAIR 10
Match: AT5G63450.1 (cytochrome P450, family 94, subfamily B, polypeptide 1 )

HSP 1 Score: 534.6 bits (1376), Expect = 7.2e-152
Identity = 272/440 (61.82%), Postives = 334/440 (75.91%), Query Frame = 0

Query: 4   LGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLAS 63
           L  RRTI+TANP NVEH+LKTNF N+PKGKPFTD+LGDLLG GIFN DG+LWS+QRKLAS
Sbjct: 78  LFGRRTIITANPENVEHILKTNFYNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLAS 137

Query: 64  HAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTLGTD 123
           H F+ +SLREF  + L +EV  RLIP+L +A      +D Q+VL R AFD VCKV+LG D
Sbjct: 138 HEFTMRSLREFTFEILREEVQNRLIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWD 197

Query: 124 EQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEW 183
             CLD++RPVPE+VKAFDVA  ISARRA  P+Y  WKVKR LN+GSEK+L++ ++TVH  
Sbjct: 198 PDCLDLTRPVPELVKAFDVAAEISARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLS 257

Query: 184 ISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDTTSA 243
           +S II  K  + +   + +  Q     DLLSR L+AG  EE +RD ++SFIMAGRDTTSA
Sbjct: 258 VSEIIRAKKKSLDIGGDVSDKQ-----DLLSRFLAAGHGEEAVRDSVISFIMAGRDTTSA 317

Query: 244 AMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLY 303
           AMTWLFWLL+ + ++E  I++E   L N      SLG G+E+L++M Y KACLCE+MRLY
Sbjct: 318 AMTWLFWLLSQNDDVETKILDE---LRNKG----SLGLGFEDLREMSYTKACLCEAMRLY 377

Query: 304 PPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQNGE- 363
           PPVAWDSKHAA  DILPDGT ++KGD+VTYFPYGMGRM+++WGKD  EFKP RWF+    
Sbjct: 378 PPVAWDSKHAANDDILPDGTPLKKGDKVTYFPYGMGRMEKVWGKDWDEFKPNRWFEEEPS 437

Query: 364 ------LKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFV 423
                 LK+VS+FKFPVFQAGPR+C+G EMAF QMKYV+ +VL RF+   V  NN PVFV
Sbjct: 438 YGTKPVLKSVSSFKFPVFQAGPRVCIGKEMAFTQMKYVVGSVLSRFKIIPVC-NNRPVFV 497

Query: 424 PLLTAHMAGGLKVYVRERTE 437
           PLLTAHMAGGLKV ++ R +
Sbjct: 498 PLLTAHMAGGLKVKIKRREQ 504

BLAST of Chy2G049230 vs. TAIR 10
Match: AT3G48520.1 (cytochrome P450, family 94, subfamily B, polypeptide 3 )

HSP 1 Score: 528.9 bits (1361), Expect = 4.0e-150
Identity = 264/438 (60.27%), Postives = 327/438 (74.66%), Query Frame = 0

Query: 4   LGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLAS 63
           LG+RRTI+T NP NVE++LKTNF N+PKGKPFTD+LGDLLG GIFNVDG  WS+QRKLAS
Sbjct: 76  LGNRRTIITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNVDGHSWSSQRKLAS 135

Query: 64  HAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTLGTD 123
           H FS +SLR F  + L+DEV  RL+P+L  AA     +DLQDVL R AFD VCKV+LG D
Sbjct: 136 HEFSTRSLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRFAFDVVCKVSLGWD 195

Query: 124 EQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEW 183
             CLD++RPV  +V+AFD A  ISARRA  P+Y  WK KR+LN+GSE+KL++ ++TVH  
Sbjct: 196 PDCLDLTRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVGSERKLREAIRTVHVL 255

Query: 184 ISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDTTSA 243
           +S I+  K  +          Q     DLLSR L+AG N E +RDM++SFIMAGRDTTSA
Sbjct: 256 VSEIVRAKKKSLEIGTGAEAKQ-----DLLSRFLAAGHNGEAVRDMVISFIMAGRDTTSA 315

Query: 244 AMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLY 303
           AMTWLFWLLT + ++E+ I+ E   L        SLG G+E+LK+M Y KACLCE+MRLY
Sbjct: 316 AMTWLFWLLTENDDVERKILEEVDPL-------VSLGLGFEDLKEMAYTKACLCEAMRLY 375

Query: 304 PPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQNGE- 363
           PPV+WDSKHAA  D+LPDGT+V++GD+VTYFPYGMGRM+ LWG D  EF P RWF +   
Sbjct: 376 PPVSWDSKHAANDDVLPDGTRVKRGDKVTYFPYGMGRMETLWGTDSEEFNPNRWFDSEPG 435

Query: 364 -----LKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVP 423
                LK +S +KFPVFQAGPR+C+G EMAF+QMKYV+ +VL RFE   V++ + PVFVP
Sbjct: 436 STRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIVPVNK-DRPVFVP 495

Query: 424 LLTAHMAGGLKVYVRERT 436
           LLTAHMAGGLKV ++ R+
Sbjct: 496 LLTAHMAGGLKVKIKRRS 500

BLAST of Chy2G049230 vs. TAIR 10
Match: AT3G01900.1 (cytochrome P450, family 94, subfamily B, polypeptide 2 )

HSP 1 Score: 453.0 bits (1164), Expect = 2.8e-127
Identity = 235/440 (53.41%), Postives = 301/440 (68.41%), Query Frame = 0

Query: 1   MHRLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRK 60
           + RL +RRT++TANP+NVE++LKTNF NYPKGKPFT+ILGD LG GIFNVDG+LW  QR+
Sbjct: 69  IRRLAARRTVVTANPSNVEYILKTNFDNYPKGKPFTEILGDFLGNGIFNVDGNLWLKQRR 128

Query: 61  LASHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTL 120
           LA+H F+ KSLRE+V   L +EV   L+  L+ AA  +   DLQ++L R  F+ VC V L
Sbjct: 129 LATHDFTPKSLREYVT-VLRNEVEKELLAFLNAAAEDSQPFDLQELLRRFTFNIVCIVFL 188

Query: 121 GTDEQCLDMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTV 180
           G D   L+ S PV E  +AF  A+A+SA R   PL   WK KR++  GSEK+L++ V  V
Sbjct: 189 GIDRCTLNPSSPVSEFDRAFQTASAVSAGRGSAPLSFVWKFKRLVGFGSEKELRKAVGEV 248

Query: 181 HEWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAGLNEEVIRDMIVSFIMAGRDT 240
           H  +  II +K             +   N D LSRL+ AG ++E +RDM++S IMAGRDT
Sbjct: 249 HNCVDEIIRDK------------KRKPANQDFLSRLIVAGESDETVRDMVISIIMAGRDT 308

Query: 241 TSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESM 300
           TSA  T LFWL+T H   E  +++E   +     +  + G+ YE LK +  LKACLCE M
Sbjct: 309 TSAVATRLFWLITGHEETEHDLVSEIRSV----KEEITGGFDYESLKKLSLLKACLCEVM 368

Query: 301 RLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQN 360
           RLYPPV WDSKHA   D LPDGT VR GDRVTYFPYGMGRM+ELWG+D  EFKP RW ++
Sbjct: 369 RLYPPVPWDSKHALTDDRLPDGTLVRAGDRVTYFPYGMGRMEELWGEDWDEFKPNRWAES 428

Query: 361 GE------LKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPV 420
            +      LK V+ FKFPVFQAGPR+CLG EMA++QMKY++A++L RFE   +   ++P 
Sbjct: 429 YDKTCCRVLKKVNPFKFPVFQAGPRVCLGEEMAYVQMKYIVASILDRFEIEPI-PTDKPD 488

Query: 421 FVPLLTAHMAGGLKVYVRER 435
           FVP+LTAHMAGG++V V  R
Sbjct: 489 FVPMLTAHMAGGMQVRVHRR 490

BLAST of Chy2G049230 vs. TAIR 10
Match: AT2G27690.1 (cytochrome P450, family 94, subfamily C, polypeptide 1 )

HSP 1 Score: 368.2 bits (944), Expect = 9.0e-102
Identity = 201/435 (46.21%), Postives = 278/435 (63.91%), Query Frame = 0

Query: 9   TILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLASHAFSA 68
           +++TANP+NVEH+LKTNF NYPKGK F+ ILGDLLG GIFN DGD W  QRKLAS    +
Sbjct: 75  SVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIFNSDGDTWRFQRKLASLELGS 134

Query: 69  KSLREFVVKTLEDEVHFRLIPLLHN-AARTNAVLDLQDVLGRLAFDTVCKVTLGTDEQCL 128
            S+R F  + ++ E+  RL+P+L + +    +VLDLQDV  R +FDT+ K++ G D  CL
Sbjct: 135 VSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVFRRFSFDTISKLSFGFDPDCL 194

Query: 129 DMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVHEWISNI 188
            +  P+ E   AFD A+ +SA+RA+ P  L WK KR+L +GSEKKL++ +  ++    ++
Sbjct: 195 RLPFPISEFAVAFDTASLLSAKRALAPFPLLWKTKRLLRIGSEKKLQESINVINRLAGDL 254

Query: 189 IHNKVLNNNDNKNHNHNQTHNNSDLLSRLLS--AGLNEEVIRDMIVSFIMAGRDTTSAAM 248
           I  + L     KN          DL+SR ++  A  ++E +RD++VSF++AGRDT +A +
Sbjct: 255 IKQRRLTGLMGKN----------DLISRFMAVVAEDDDEYLRDIVVSFLLAGRDTVAAGL 314

Query: 249 TWLFWLLTNHLNIEQTIINE--ATLLSNHDSKTTSLGYGYEELKDMKYLKACLCESMRLY 308
           T  FWLLT H  +E  I  E    + +  DS T       +E+++M YL A L ESMRL+
Sbjct: 315 TGFFWLLTRHPEVENRIREELDRVMGTGFDSVTARC----DEMREMDYLHASLYESMRLF 374

Query: 309 PPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQN-GE 368
           PPV +DSK A   D+L DGT V  G RVTY  Y MGRM  +WG D  EFKPERW  N G+
Sbjct: 375 PPVQFDSKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEGK 434

Query: 369 LKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVS-ENNEPV-FVPLLT 428
            +  +  K+PVFQAG R+C+G EMA ++MK +   +++RFE R  S E  E + F P LT
Sbjct: 435 FRPENPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRVASPETTETLRFAPGLT 494

Query: 429 AHMAGGLKVYVRERT 436
           A + GGL V ++ER+
Sbjct: 495 ATVNGGLPVMIQERS 495

BLAST of Chy2G049230 vs. TAIR 10
Match: AT3G56630.1 (cytochrome P450, family 94, subfamily D, polypeptide 2 )

HSP 1 Score: 368.2 bits (944), Expect = 9.0e-102
Identity = 190/438 (43.38%), Postives = 275/438 (62.79%), Query Frame = 0

Query: 3   RLGSRRTILTANPANVEHMLKTNFLNYPKGKPFTDILGDLLGCGIFNVDGDLWSTQRKLA 62
           R G  + ++TANPANVE+MLKT F ++PKG+ F  IL D LG GIFN DG++W  QRK A
Sbjct: 70  RPGKLQFVMTANPANVEYMLKTKFESFPKGERFISILEDFLGRGIFNSDGEMWWKQRKTA 129

Query: 63  SHAFSAKSLREFVVKTLEDEVHFRLIPLLHNAARTNAVLDLQDVLGRLAFDTVCKVTLGT 122
           S+ FS KSLR+FV+  +  E++ RL+P+L  AA    ++DLQD+L R AFD +CK+    
Sbjct: 130 SYEFSTKSLRDFVMSNVTVEINTRLVPVLAEAATNGKLIDLQDILERFAFDNICKLAFNV 189

Query: 123 DEQCL-DMSRPVPEIVKAFDVATAISARRAVTPLYLTWKVKRMLNLGSEKKLKQVVQTVH 182
           D  CL D        ++AF+ A  I ++R  + +  +WK+K+ LN+GSE+ L++ +  VH
Sbjct: 190 DSACLGDDGAAGVNFMQAFETAATIISQRFQSVISYSWKIKKKLNIGSERVLRESIMIVH 249

Query: 183 EWISNIIHNKVLNNNDNKNHNHNQTHNNSDLLSRLLSAG--LNEEVIRDMIVSFIMAGRD 242
           ++   I+ N++      K  +H +     DLLSR +S     + E++RD+++SFI+AGRD
Sbjct: 250 KFADEIVRNRI---EQGKVSDHKE-----DLLSRFISKEEMNSPEILRDIVISFILAGRD 309

Query: 243 TTSAAMTWLFWLLTNHLNIEQTIINEATLLSNHDSKTTSLGYGYEELKDMKYLKACLCES 302
           TTS+A++W FWLL+ H  ++  I+ E   +     K     YG+E+LK M YL A + ES
Sbjct: 310 TTSSALSWFFWLLSMHPEVKDKILQELNSIRERTGKRIGEVYGFEDLKLMNYLHAAITES 369

Query: 303 MRLYPPVAWDSKHAAAADILPDGTQVRKGDRVTYFPYGMGRMKELWGKDRLEFKPERWFQ 362
           +RLYPPV  D+   A  ++LPDGT + K   ++Y  Y MGRM+ +WGKD   F PERW  
Sbjct: 370 LRLYPPVPVDTMSCAEDNVLPDGTFIGKDWGISYNAYAMGRMESIWGKDCDRFDPERWID 429

Query: 363 --NGELKTVSAFKFPVFQAGPRMCLGTEMAFIQMKYVMATVLKRFEFRRVSENNEPVFVP 422
             NG  +  + +KFP F AGPRMCLG EMA+IQMK ++A VL+RF      +   P  + 
Sbjct: 430 ETNGGFRGENPYKFPAFHAGPRMCLGKEMAYIQMKSIVAAVLERFVVEVPGKKERPEILM 489

Query: 423 LLTAHMAGGLKVYVRERT 436
            +T  + GGL V V+ER+
Sbjct: 490 SVTLRIRGGLNVRVQERS 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMV71.0e-15061.82Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1[more]
Q9SMP55.6e-14960.27Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1[more]
Q9ZUX11.3e-10046.21Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1[more]
O811171.2e-9843.81Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2[more]
P981887.6e-9843.89Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LR921.0e-24697.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G002090 PE=4 SV=1[more]
A0A5D3BH585.3e-23593.84Cytochrome P450 94B3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350... [more]
A0A1S3CN075.3e-23593.84cytochrome P450 94B3 OS=Cucumis melo OX=3656 GN=LOC103502328 PE=4 SV=1[more]
A0A6J1E0I03.8e-20983.52cytochrome P450 94B3 OS=Cucurbita moschata OX=3662 GN=LOC111429724 PE=3 SV=1[more]
A0A6J1JAK95.5e-20883.52cytochrome P450 94B3-like OS=Cucurbita maxima OX=3661 GN=LOC111485059 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011651847.12.01e-31297.95cytochrome P450 94B3 [Cucumis sativus] >KGN63489.1 hypothetical protein Csa_0136... [more]
KAA0057776.13.75e-29793.84cytochrome P450 94B3 [Cucumis melo var. makuwa] >TYJ98457.1 cytochrome P450 94B3... [more]
XP_008464443.14.52e-29793.84PREDICTED: cytochrome P450 94B3 [Cucumis melo][more]
XP_038879988.14.08e-27386.73cytochrome P450 94B3-like [Benincasa hispida][more]
XP_023516482.13.01e-26584.21cytochrome P450 94B3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G63450.17.2e-15261.82cytochrome P450, family 94, subfamily B, polypeptide 1 [more]
AT3G48520.14.0e-15060.27cytochrome P450, family 94, subfamily B, polypeptide 3 [more]
AT3G01900.12.8e-12753.41cytochrome P450, family 94, subfamily B, polypeptide 2 [more]
AT2G27690.19.0e-10246.21cytochrome P450, family 94, subfamily C, polypeptide 1 [more]
AT3G56630.19.0e-10243.38cytochrome P450, family 94, subfamily D, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 372..381
score: 46.46
coord: 381..392
score: 32.26
coord: 294..305
score: 38.04
coord: 236..253
score: 36.98
IPR001128Cytochrome P450PFAMPF00067p450coord: 3..413
e-value: 1.0E-61
score: 209.1
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 381..399
score: 29.76
coord: 227..253
score: 21.51
coord: 323..337
score: 28.97
coord: 289..305
score: 31.45
coord: 340..358
score: 23.48
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..437
e-value: 8.1E-88
score: 297.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 3..434
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 1..432
NoneNo IPR availablePANTHERPTHR24296:SF5CYTOCHROME P450 94B3coord: 1..432

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G049230.1Chy2G049230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen