Chy2G047500 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G047500
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	PMEI domain-containing protein
Location	chrH02: 28367890 .. 28369825 (-)
RNA-Seq Expression	Chy2G047500
Synteny	Chy2G047500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATCAATATATCTACTTCCTTTCTCTTCTCCTTCACTATCTTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACAGCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGATTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAGAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGTAAGTTCAATTATGGAAGCTCCTACAACTTGTGAAAATGGGTTTAAAGAAAAAGAAGGAATCATTTCGCCATTGACAAAGAATAATACTAATGTGTTTCAATTGGCAGCTTTGACTCTCTCCATCATCAATATGAATCTGCATGATATCCAATAATAATCTTTATAATAGTAATTTCATCATAGAATTTATGTCATTGGCGAATATATGTTGTTTCTTATTTATTCCACTTTATTGTATACAATTGATCAGTGAGAGATTGAGGAGATGTTTTTAATTGTGTGGGAATTATTGATTTTAATTTGAGGACTCATGTCGACATGTGACTCAACCATGATTTTTTAGTAGGAAAATACTTGCACGTACCTTAGAGTACTAAACATATCTTGTTGTATTCTATTAAAAAGATTGTGTGATACAAGATGGAACGAGCAAAGATAAGTTCATCTTGATACTACAATTAATTGGTAGTTGAGTTGCAATTGCAATCATTCTTTGTAATCTCTCTAACCATAAAATAAAATATTATATATGTAAAAAAGTTTGTCACATGTCACTTAAACCAAACCGAAAAGATATATAGTTGGATAGTATGGGTGAGTGAGAACTTTTCTAGTTTTACAAAAGTGTTATAACTCTCGCTTTCTTCTCTCTAAAAGTTTGTCTTCATAAATACTTTATTTTTTTGTGTGTGTTAAGTAATATACTTCATACTATTTGATATGTAATAAATGGAAGTTGTATAGAAAACTTTACTCTTAAACTAAATTCAAAAAAAGCTTAGAAAATACACATGTGAATAGTTGGTTAATTTGGGTTTGATGGAGAAATTAAAATAGGGTAGAGGTAACCATATTTAGAATTTCTTTTATAGAAATATATATAGCCCAAATGGTGTGTAAACTTAAAACATAACTTTTGTTGTTCTTACCTAATTAGTCCAATATTCATATTTACTTTCTAATTTTTTTAACTTGTGTAAAAAAAAAAAGTGTATAGTGTGGTCGTTGATCGTTTAATTTTTTCCTAACTACGTGTTTGAATTTGAACTATTCATAAACTCTTAAATATTTAAGTTTTGGGTTCAATACATATTTGTAATTTTTTTTAAAATATTGTATAATATATTAAGAATCTAATGAAAAGAAAATTGACACAAAAAAGTTAAAAGTTTACTACTCTATCCGAAATTTTTTAAGTTGTTGAATATTAAATAAATACAAATTTCAAAATTCGTAAATTAAAACTATAATTCAACCATAGCATTTTAAAATCAATTTACAACCCAACAAGTTAGTCATTTTGAGTGATTGTTAAATAACAATAATAATAGAGAAATTTTTATCTATAGAAAAATGCCAAACTATTTAAAGAAAAAAGTAAAAAAAATACTGATTGAGGTTGATATCCTTCTATCATCATTAATAAGTTTTTGTTATTTTTCTATATCTTCAAAGCCCTTAATAAATATATATAGGAAGAAGAAAACGTGGGAATGAGGGGAAGAGGTGAATGA

mRNA sequence

ATGATGATCAATATATCTACTTCCTTTCTCTTCTCCTTCACTATCTTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACAGCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGATTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAGAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGAAGAAGAAAACGTGGGAATGAGGGGAAGAGGTGAATGA

Coding sequence (CDS)

ATGATGATCAATATATCTACTTCCTTTCTCTTCTCCTTCACTATCTTCTTTATACTCATTTTTTCCTCATTCTCCCAAACCTATTCAAATCCCCACAATATCATTCAAGAAACATGCAAGAAATCAGCTGCGAGTACCCCAAATCTCACCTACAAATTCTGTGTTACTTCTTTAGAATCCGACAGCCGTAGTCGCTATGCGAATCTTCACAAACTTGGCCTCATTTCCATGGATTTGTTGCGACACAACGTAACAAGCACTCGACGAGAAATCAAGAAGCTTTTGAGGAACAAAAAAATGGAAGAGTTCATCAAGGGTTGTTTGAAGGATTGTGTAGAGCTTTATTCTGATGCTGTTCCAACATTGAAAGAAGCAAAAAGAGAATACAAAAATAGGAATTATAAAGATGCAAATATTAAGGAAGAAGAAAACGTGGGAATGAGGGGAAGAGGTGAATGA

Protein sequence

MMINISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIKEEENVGMRGRGE*

Homology

[Close]

BLAST of Chy2G047500 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.2e-13
Identity = 48/130 (36.92%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 11  FSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYANLH 70
           FS  IFF++             +I+Q TCKK A  +PN+ Y FCV SL +D +S  A+L 
Sbjct: 5   FSLCIFFLI-----------SADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQ 64

Query: 71  KLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYK 130
            LG+IS +L     +  +  I ++L++ K++  +K  L DCV LY+DA  +++EA  ++K
Sbjct: 65  GLGVISANLAIQQGSKIQTFIGRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFK 122

Query: 131 NRNYKDANIK 141
           +++Y  AN+K
Sbjct: 125 SKDYASANVK 122

[Close]

BLAST of Chy2G047500 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.6e-13
Identity = 49/130 (37.69%), Postives = 79/130 (60.77%), Query Frame = 0


Query: 11  FSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYANLH 70
           FS  IFF  +        S   +I+Q TCKK A  +PN+ Y FCV SL +D +S  A+L 
Sbjct: 5   FSLCIFFFNLLLLLQAVIS--ADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQ 64

Query: 71  KLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYK 130
            LG+IS +L   + +  +  I ++L++ K++  +K  L DCV LY+DA  +++EA  ++K
Sbjct: 65  GLGVISANLAIQHGSKIQTFIGRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFK 124

Query: 131 NRNYKDANIK 141
           +++Y  AN+K
Sbjct: 125 SKDYASANVK 131

[Close]

BLAST of Chy2G047500 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-09
Identity = 43/136 (31.62%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 18  ILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYA-NLHKLGLIS 77
           ++IFS F   ++    +IQ++CKK+ A  P L+Y FCV SL  D +S+ A  L  L L S
Sbjct: 7   LVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLAS 66

Query: 78  MDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKD 137
                  +T+ +  + + L++++ ++ ++  LK C+  Y+DA   L  A    K+R+Y+ 
Sbjct: 67  TKTAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRDYQG 126

Query: 138 ANIKEEENVGMRGRGE 153
           ANI     + + G  E
Sbjct: 127 ANINLSAALDVPGNCE 141

[Close]

BLAST of Chy2G047500 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 7.7e-05
Identity = 44/141 (31.21%), Postives = 72/141 (51.06%), Query Frame = 0


Query: 1   MMINISTSFLFSFTIFFILIFSSFSQ--TYSNPHNIIQETCKKSAASTPNLTYKFCVTSL 60
           M  +  +S LF   +  IL  S  SQ  +    +++I E C K+    P+L    C+ +L
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKT--RNPSL----CLQAL 60

Query: 61  ESDSRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDA 120
           ESD RS   +L  LG  S+D+ + +   T + I   L N+  +  +KG  + C E Y+DA
Sbjct: 61  ESDPRSASKDLKGLGQFSIDIAQASAKQTSKIIAS-LTNQATDPKLKGRYETCSENYADA 120

Query: 121 VPTLKEAKREYKNRNYKDANI 140
           + +L +AK+   + +Y   NI
Sbjct: 121 IDSLGQAKQFLTSGDYNSLNI 134

[Close]

BLAST of Chy2G047500 vs. ExPASy Swiss-Prot
Match: O49603 (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702 GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 6.5e-04
Identity = 41/135 (30.37%), Postives = 65/135 (48.15%), Query Frame = 0


Query: 5   ISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRS 64
           +++S +F   +      S+     SN   II+ TCK     T N  YKFCV++L+SD RS
Sbjct: 1   MASSLIFLLLVTLTFSASTLISAKSNTTTIIESTCK-----TTNY-YKFCVSALKSDPRS 60

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
             A+   L  I + +   N TST   I   L     +  +K  L+DC E Y+ A  +L+ 
Sbjct: 61  PTADTKGLASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADSLRL 120

Query: 125 AKREYKNRNYKDANI 140
             ++  +  Y  A++
Sbjct: 121 TIQDLDDEAYDYASM 129

[Close]

BLAST of Chy2G047500 vs. ExPASy TrEMBL
Match: A0A0A0LUJ3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-65
Identity = 134/136 (98.53%), Postives = 135/136 (99.26%), Query Frame = 0


Query: 5   ISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRS 64
           ISTSFLFSFTI FILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESD+RS
Sbjct: 2   ISTSFLFSFTIVFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDTRS 61

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
           RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE
Sbjct: 62  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 121

Query: 125 AKREYKNRNYKDANIK 141
           AKREYKNRNYKDANIK
Sbjct: 122 AKREYKNRNYKDANIK 137

[Close]

BLAST of Chy2G047500 vs. ExPASy TrEMBL
Match: A0A6J1EZS0 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 4.1e-41
Identity = 90/136 (66.18%), Postives = 111/136 (81.62%), Query Frame = 0


Query: 5   ISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRS 64
           IST  LF  +   +++ SSFS   S PHN++QETCKK AA+TPNL+Y FCV+SLESD RS
Sbjct: 2   ISTFSLFFLSPLLVILVSSFSHG-STPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 61

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
           R A+LH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K CL DC+ELYSDA+PTLKE
Sbjct: 62  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 121

Query: 125 AKREYKNRNYKDANIK 141
            KREYK + +  AN+K
Sbjct: 122 CKREYKQKRFSGANVK 136

[Close]

BLAST of Chy2G047500 vs. ExPASy TrEMBL
Match: A0A6J1I7I4 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.2e-40
Identity = 91/136 (66.91%), Postives = 111/136 (81.62%), Query Frame = 0


Query: 5   ISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRS 64
           IST  LF  +  F+++ SS S   S PHN++QETCKK AA+TPNL+Y FCV+SLESD RS
Sbjct: 2   ISTFSLFFLSPLFVILVSSSSHG-STPHNMVQETCKKCAATTPNLSYSFCVSSLESDDRS 61

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
           R ANLH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K  L DC+ELYSDA+PTLKE
Sbjct: 62  RSANLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKVRLDDCLELYSDAIPTLKE 121

Query: 125 AKREYKNRNYKDANIK 141
            KREYK + + DAN+K
Sbjct: 122 CKREYKQKRFSDANVK 136

[Close]

BLAST of Chy2G047500 vs. ExPASy TrEMBL
Match: A0A6J1C4E5 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 3.8e-39
Identity = 93/139 (66.91%), Postives = 111/139 (79.86%), Query Frame = 0


Query: 3   INISTSFLFSFTIFFILIFSSFSQ-TYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESD 62
           I IST   FS T FF ++F SFS  +     NIIQETCK+SA STPNL+Y FCV SLESD
Sbjct: 26  IMIST---FSLTTFFAVLFFSFSDGSTITTSNIIQETCKRSADSTPNLSYNFCVASLESD 85

Query: 63  SRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPT 122
            RSR A+LHKLGLI+MDL+RHN+TSTRR+I+KLLRNKK+++F+K  L DC+ELYSDAVP 
Sbjct: 86  DRSRCADLHKLGLIAMDLMRHNITSTRRDIRKLLRNKKLDQFVKARLDDCLELYSDAVPI 145

Query: 123 LKEAKREYKNRNYKDANIK 141
           LKEAKR YK + Y DAN+K
Sbjct: 146 LKEAKRCYKEKKYVDANVK 161

[Close]

BLAST of Chy2G047500 vs. ExPASy TrEMBL
Match: A0A4U5QXE7 (Invertase/pectin methylesterase inhibitor family protein OS=Populus alba OX=43335 GN=D5086_0000027800 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 2.7e-29
Identity = 66/122 (54.10%), Postives = 96/122 (78.69%), Query Frame = 0


Query: 19  LIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYANLHKLGLISMD 78
           L+FS+F+   +   N+I ETCKK AA  PNL+Y FCVTSL++ +RS+  NL  LG++S+ 
Sbjct: 15  LLFSAFNANIA--QNLIPETCKKCAADDPNLSYNFCVTSLQASNRSQCDNLRGLGMMSIK 74

Query: 79  LLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDAN 138
           L+++NVT+TR  +K LL+NKK + FI+ CL DC++LYSDA+PTLK+A  +YK+++YKDAN
Sbjct: 75  LIKYNVTNTRNYVKNLLKNKKTDPFIRACLNDCLDLYSDAIPTLKQAMMDYKSKHYKDAN 134

Query: 139 IK 141
           I+
Sbjct: 135 IE 134

[Close]

BLAST of Chy2G047500 vs. NCBI nr
Match: XP_038880152.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 214 bits (545), Expect = 4.98e-68
Identity = 113/139 (81.29%), Postives = 124/139 (89.21%), Query Frame = 0


Query: 2   MINISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESD 61
           MI ISTS LFSFT F +LIFSS S   SN HNIIQETCKKSA S+PN++YKFCV S ESD
Sbjct: 1   MIMISTSHLFSFTAFLLLIFSSSSHG-SNTHNIIQETCKKSAESSPNISYKFCVASFESD 60

Query: 62  SRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPT 121
           +RSR ANLHKLGLISMDL+RHN+TSTRREIKKLLRNKK+EEFIKGCLKDC+ELYSDAVPT
Sbjct: 61  NRSRSANLHKLGLISMDLMRHNITSTRREIKKLLRNKKLEEFIKGCLKDCLELYSDAVPT 120

Query: 122 LKEAKREYKNRNYKDANIK 140
           LKEAKREYK + YKDAN+K
Sbjct: 121 LKEAKREYKEKKYKDANVK 138

[Close]

BLAST of Chy2G047500 vs. NCBI nr
Match: KAE8652421.1 (hypothetical protein Csa_013805 [Cucumis sativus])

HSP 1 Score: 204 bits (520), Expect = 1.23e-64
Identity = 103/104 (99.04%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 37  ETCKKSAASTPNLTYKFCVTSLESDSRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLR 96
           ETCKKSAASTPNLTYKFCVTSLESD+RSRYANLHKLGLISMDLLRHNVTSTRREIKKLLR
Sbjct: 5   ETCKKSAASTPNLTYKFCVTSLESDTRSRYANLHKLGLISMDLLRHNVTSTRREIKKLLR 64

Query: 97  NKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIK 140
           NKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIK
Sbjct: 65  NKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKDANIK 108

[Close]

BLAST of Chy2G047500 vs. NCBI nr
Match: XP_022933437.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 176 bits (446), Expect = 5.17e-53
Identity = 90/136 (66.18%), Postives = 111/136 (81.62%), Query Frame = 0


Query: 5   ISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRS 64
           IST  LF  +   +++ SSFS   S PHN++QETCKK AA+TPNL+Y FCV+SLESD RS
Sbjct: 2   ISTFSLFFLSPLLVILVSSFSHG-STPHNMVQETCKKCAATTPNLSYAFCVSSLESDDRS 61

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
           R A+LH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K CL DC+ELYSDA+PTLKE
Sbjct: 62  RSASLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKACLDDCLELYSDAIPTLKE 121

Query: 125 AKREYKNRNYKDANIK 140
            KREYK + +  AN+K
Sbjct: 122 CKREYKQKRFSGANVK 136

[Close]

BLAST of Chy2G047500 vs. NCBI nr
Match: XP_022973011.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 174 bits (442), Expect = 2.09e-52
Identity = 91/136 (66.91%), Postives = 111/136 (81.62%), Query Frame = 0


Query: 5   ISTSFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRS 64
           IST  LF  +  F+++ SS S   S PHN++QETCKK AA+TPNL+Y FCV+SLESD RS
Sbjct: 2   ISTFSLFFLSPLFVILVSSSSHG-STPHNMVQETCKKCAATTPNLSYSFCVSSLESDDRS 61

Query: 65  RYANLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKE 124
           R ANLH+LGLISMDLLRHN+TSTRRE+KKLLRNK ++ F+K  L DC+ELYSDA+PTLKE
Sbjct: 62  RSANLHRLGLISMDLLRHNLTSTRREVKKLLRNKTLDGFVKVRLDDCLELYSDAIPTLKE 121

Query: 125 AKREYKNRNYKDANIK 140
            KREYK + + DAN+K
Sbjct: 122 CKREYKQKRFSDANVK 136

[Close]

BLAST of Chy2G047500 vs. NCBI nr
Match: XP_022135283.1 (putative invertase inhibitor [Momordica charantia])

HSP 1 Score: 170 bits (430), Expect = 2.98e-50
Identity = 89/131 (67.94%), Postives = 107/131 (81.68%), Query Frame = 0


Query: 11  FSFTIFFILIFSSFSQTYS-NPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYANL 70
           FS T FF ++F SFS   +    NIIQETCK+SA STPNL+Y FCV SLESD RSR A+L
Sbjct: 31  FSLTTFFAVLFFSFSDGSTITTSNIIQETCKRSADSTPNLSYNFCVASLESDDRSRCADL 90

Query: 71  HKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREY 130
           HKLGLI+MDL+RHN+TSTRR+I+KLLRNKK+++F+K  L DC+ELYSDAVP LKEAKR Y
Sbjct: 91  HKLGLIAMDLMRHNITSTRRDIRKLLRNKKLDQFVKARLDDCLELYSDAVPILKEAKRCY 150

Query: 131 KNRNYKDANIK 140
           K + Y DAN+K
Sbjct: 151 KEKKYVDANVK 161

[Close]

BLAST of Chy2G047500 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-16
Identity = 50/133 (37.59%), Postives = 81/133 (60.90%), Query Frame = 0


Query: 8   SFLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYA 67
           +FLF F +FF+L+       ++   ++IQ+ CKK+A   P + Y FCV SLE + +S+ A
Sbjct: 2   TFLFYFAMFFLLL-----NAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTA 61

Query: 68  -NLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAK 127
            +L +L ++S        TS +  + K+L+  + E + +  L+DC+ELYSDA  +LKEA 
Sbjct: 62  RSLDRLVMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEAL 121

Query: 128 REYKNRNYKDANI 140
              K+R+YK AN+
Sbjct: 122 TIIKSRDYKTANV 129

[Close]

BLAST of Chy2G047500 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.1e-11
Identity = 42/127 (33.07%), Postives = 72/127 (56.69%), Query Frame = 0


Query: 9   FLFSFTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYA- 68
           FL     FF+L     S   +N   +I+ +CKK+ A++P   Y  CVTSLE++ +++ A 
Sbjct: 3   FLLYLVTFFVL-----SNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAK 62

Query: 69  NLHKLGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKR 128
           +L  L + S        T+ +  + K+++ KK+ +     L+DC++LY+DA+ +L EA  
Sbjct: 63  DLAGLVMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALA 122

Query: 129 EYKNRNY 135
             K+RNY
Sbjct: 123 GVKSRNY 124

[Close]

BLAST of Chy2G047500 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 9.7e-11
Identity = 43/136 (31.62%), Postives = 74/136 (54.41%), Query Frame = 0


Query: 18  ILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYA-NLHKLGLIS 77
           ++IFS F   ++    +IQ++CKK+ A  P L+Y FCV SL  D +S+ A  L  L L S
Sbjct: 7   LVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLAS 66

Query: 78  MDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKD 137
                  +T+ +  + + L++++ ++ ++  LK C+  Y+DA   L  A    K+R+Y+ 
Sbjct: 67  TKTAAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYNDANDDLTTALANIKSRDYQG 126

Query: 138 ANIKEEENVGMRGRGE 153
           ANI     + + G  E
Sbjct: 127 ANINLSAALDVPGNCE 141

[Close]

BLAST of Chy2G047500 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.3e-10
Identity = 40/122 (32.79%), Postives = 69/122 (56.56%), Query Frame = 0


Query: 18  ILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYA-NLHKLGLIS 77
           ++IFS F   ++    +IQ++CKK+ A  P  +Y FCV SL  D +S+ A  L  L L S
Sbjct: 7   LVIFSLFLNGFATAQTLIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATTLEDLALAS 66

Query: 78  MDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKNRNYKD 137
              +   +T+ +  + + L++++ ++ ++  LK C+  Y DA  +LK A    K+R+Y  
Sbjct: 67  TKNVAAKITNLKGIVAQDLKDQRYQDIVED-LKLCLGFYKDANDSLKTALANIKSRDYDG 126

Query: 138 AN 139
           AN
Sbjct: 127 AN 127

[Close]

BLAST of Chy2G047500 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 2.8e-10
Identity = 39/128 (30.47%), Postives = 74/128 (57.81%), Query Frame = 0


Query: 13  FTIFFILIFSSFSQTYSNPHNIIQETCKKSAASTPNLTYKFCVTSLESDSRSRYA-NLHK 72
           F+++ ++ F   +  ++   ++I+++CKK+    P L Y FCV SLE + +S+ A +L  
Sbjct: 3   FSLYLVMFFLLLN-CFATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEG 62

Query: 73  LGLISMDLLRHNVTSTRREIKKLLRNKKMEEFIKGCLKDCVELYSDAVPTLKEAKREYKN 132
           L  +S        TS +  + K+L+  K E  ++  L DC+ELY++A+ +L ++    K+
Sbjct: 63  LVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAIDSLNQSLDTVKS 122

Query: 133 RNYKDANI 140
           R+YK A +
Sbjct: 123 RDYKTATM 127

The following BLAST results are available for this feature:

BLAST of Chy2G047500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A9YUH4	1.2e-13	36.92	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q8GT41	1.6e-13	37.69	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
Q9FJR5	1.4e-09	31.62	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
P83326	7.7e-05	31.21	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
O49603	6.5e-04	30.37	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Chy2G047500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUJ3	1.4e-65	98.53	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009900 PE=4 S...	[more]
A0A6J1EZS0	4.1e-41	66.18	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111440851 PE=4 ...	[more]
A0A6J1I7I4	1.2e-40	66.91	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471526 PE=4 SV...	[more]
A0A6J1C4E5	3.8e-39	66.91	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111007285 PE=4...	[more]
A0A4U5QXE7	2.7e-29	54.10	Invertase/pectin methylesterase inhibitor family protein OS=Populus alba OX=4333...	[more]

BLAST of Chy2G047500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038880152.1	4.98e-68	81.29	putative invertase inhibitor [Benincasa hispida]	[more]
KAE8652421.1	1.23e-64	99.04	hypothetical protein Csa_013805 [Cucumis sativus]	[more]
XP_022933437.1	5.17e-53	66.18	putative invertase inhibitor [Cucurbita moschata]	[more]
XP_022973011.1	2.09e-52	66.91	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_022135283.1	2.98e-50	67.94	putative invertase inhibitor [Momordica charantia]	[more]

BLAST of Chy2G047500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46970.1	2.0e-16	37.59	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46940.1	1.1e-11	33.07	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	9.7e-11	31.62	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	1.3e-10	32.79	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	2.8e-10	30.47	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 77..97
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 2..140
None	No IPR available	PANTHER	PTHR31707:SF314	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR	coord: 2..140
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..147 e-value: 8.6E-4 score: 28.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..137 e-value: 1.3E-17 score: 64.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..140 e-value: 1.4E-30 score: 103.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 31..144 e-value: 6.6E-26 score: 93.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..139
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 39..143 e-value: 2.75971E-35 score: 117.851

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G047500.1	Chy2G047500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004857	enzyme inhibitor activity