Chy2G047070 (gene) Cucumber (hystrix) v1

Overview
NameChy2G047070
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionReplication protein A subunit
LocationchrH02: 28056017 .. 28059440 (-)
RNA-Seq ExpressionChy2G047070
SyntenyChy2G047070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGTGCACCAACTGCTGGCGGAATATCAAAGGTTTTGTCCAATCCATCACCGGACTCCCCCTCCGATGTCCCCGACGTTGTGGTTCAGGTCATTGATCTCAAGGCAACGGGAAATCGCTATATGTGAGTTTTCGTTCACGAATAAACTCTGATTAGTACTCTGTTTTTGTGTATATTTGCTTGCAATGTGTGAGGTTCGCTTTTGGCTTTGACCTAAGTCTGATGAAGTTCTTATTTAGACGATTTGAAGTTGAAAGTATGGTTTGGATCTCAGTGTTTGTGTTGTTTGTTATATATTCTGTTTATGGTTTTGACTTTGCAGCTGAGGGTATGTGAGTTTTCTTTTGCTTGGAATTCTGATTCGTCATGTATGTTTGCTCACACTGTGCGAGATTCACTTTTGGTTCTGACCTAAGTTTGGTGAAGTTCTTGTTTAGATGACTGGAAGTTGAAAGTATGGTTTAGTACTCTGTTGTTATGCATATTTGCTCGCACTGTGTGAGGTTCGCCTTTGGGTTTGACCTAAGTTTGGTGAAGTTCTTATTTAGATGACTGAAAGCTGAAGCTATGGTTTAGATCTCAGTGATTGTGTTGCTTGTGATATAGGGTGTTTATGGTTTTGAATTTGCAACCTAGCGCGTATACTGACTGGTGCAATATGATTTTTTTTTTGTTTGAAACAATTGACGATTTAAAGATGGGTGAGGTTTCTGTTGTTTTAAATGAAGATTTAAAGGCAATACTGACGGTTTCTCTATCTTCTTTTTGCTTCACATTCAGGTTTACTGCTAGCGATGGTGACATGAAGCTTAAAGCTATTCTTCCATCAAATCTGACTTCTGACGTCATTTCTGGAAACATTCAAAATTTGGGGCTTATTCGCATTCTTGACTACTCTCTCAATGATATTCCAAACAAGTCAGAAAAGTGAGTATAATCAATCAAAATTTGTAAAAGAAACTCGTGTTAGATACCTGATACTAGAAAACGTTCTTCTAGTTATTTTTCTTTTCGTTCTGTTTTGTAACAAGTGATGCTTTTCTTAATATCAGGTATTTAATCGTGACAAAATGTGAGGTGGTTTCTCCTGCTCTAGAAAAGGAGGTAAAAACTGAGGTTAAGACAGAGGAAGCAGGTACTATTTTGAAACCGAAGGTAGAGCTTGATGATAAGAGCACATTTAACAATGGATCTGGTATTATTCTGAAACCAAAGCAAGAATTGGTCGCTAAATCAGCTGCTCAGATTGTACACGAGCAGCGTATGAAGTGAGTATGCTATATTTGAAAAAGGATTTATTGGATTTTGTTATCAAGTTCCTAGACTTTTTGTGTGCAATTAATAATATATATTTAAATGCTTCTGTTTTTCAATGTGCAGTATGGCACCAGCTGCCCGGATGTCCATGACAAGGCGAGTTCAGCCCCTTGTCTACTTAAACCCTTACCTGGGAGATTGGACCATCAAAGTTCGTGTTACTGATAAGGGAAACCTGCGTACATACAAAAATGCTCAAGGAGAAGGTTGTGTTTTCAATGTGGTGTTGACAGATGAAGATGTAAGCTAACTGCTGCTTGTTGAAGTCTTGGTATTTTTTTACTGAAACAATAACATTTTCTAGTCTCATGTTGATATCCATGTGAACTAACTTGGTTCTTCTGGTTCAGGGTACACAAATACAGGCAACGATGTTCAATGATGCTGCAAGGAAGTTCTATGAGAAGTTTGCCCTCGGCAAAGTCTATTATGTTTCCAGGGGAACTTTGAAAGTTGCCAATAAGAAATTCAAGACAGTTCAAAATGATTATGAAATGACTTTGAATGTGAATTCTCAAGTAGACGAGGTCAGCAATGAAGCTGCTTTTGTTCCTGAAACAAAATATAATTTTGTCAAGATTGATATGTTAGGTCCTCACGTGAATGGAAGAGATCTCGTTGGTAAGAATCCAACCCTAGCCTAAATAATTTATCCTCGCATGATTTTATTTTATTTTTTCACTTAAAAGGTTCAAATGTGTTTTTGCGACTGCCAGATGTAATTGGAGTTGTTCAAAATGTTTCTTCTACAATGAGCATTCGAAGGAAACTCAACAATGAGACAGCTCCAAAACGTGATGTAACCATTGCTGATGAAACGTGAGTAGATGTTTTCTATTGATGAAAATGAAAATTGTTGTTATTTAATTAATTCATATTAACGTTGTGAGTATCAAATCGCAGGAAGAAGACAGTTGTGATATCTTTGTGGAATGATCTTGCAACCACTGTGGGGCAGGAGTTGCTTGACATTGCCGACCAATCTCCTATAATTGCATTGAAATATCTTAGAGTTGGAGACTTTCAAGGTACTGCTAAAATTGAAGTGGGAACTCATGTCTTCTGTGGCGAGGTGCCTACATGTACTTAACATATTATTTGCTTCCAATTTCAGGTGTATCTTTGTCGACAATAAGCAGGAGCTCAATTACAATAAACCCAGACACAAAGGAAGCACATGATTTGAGATCATGGTATGGTTACAAAGTACAAATGGTGTTACTACCGTAACATTATTTACTGTAGTTGTAATCATTCTATATATGCGTACAGGTATGACTCTGAAGGTAAAGCCGTATCCATGTCTTCTGTAAGCTCCGGCCTGAGTCCATCAACCAAGAGCGGTTCTAGGTCCATGTACTCTGACCGGATTTCCTTATCTCATATACTCGAAAACAAATCTTTGGGAGAAGAAAAGGTAACACATAATCTTCAAAATAAAGAAAGTGAATTCTATTAAGAACAAAACTACTAAATGCATGGTTTAAGGATTTTATGCCAAAAAATAACATGAAGTTATTGTCTCCACAGCCCACGTTCTTCAGCATTAGAGCATATGTCAGCTTTATCAAACCCGATCAAACAATGTGGTATCGTGCTTGCAAAACATGCAATAAGAAGGTAACTGAAGCCATCGGATCTGGATATTGGTGTGACAATTGCCAGAAAAACGATGAAGAATGCAGTTTAAGGTACATAATGGTTGTGAGGGTATCTGATGCAAGCGGTGAAGCTTGGGTCTCTACATTCAACGAAGAAGCAGAGAGAATTATGGGATGCTCAGCTGATGAACTTGATCAGCTCAAATCGCAGGTAAACAAACCATACAACACAATCAACTTTGTTGAAAACTAGAAAGAAAAAAAGCAAGTAGGTTGATCTATACAGCATGTTTTCATTTTCAGATTGGTGAGGAGAATTCATATCAACTTAAACTCAAAGAAGCCACATGGGTTCCCCACTTGTTTCGAGTTAGCGTTTCTCAGAACGAATACAACAATGAGAAGAGACAAAGGATCACCGTCAGGTCAGTTGCTCCAGTTGATTTTGCTGCTGAGTCCAGATTTTTGCTAGAAGAGATAGCAAAAATGAAGGCTTCCTAA

mRNA sequence

ATGGCGAGTGCACCAACTGCTGGCGGAATATCAAAGGTTTTGTCCAATCCATCACCGGACTCCCCCTCCGATGTCCCCGACGTTGTGGTTCAGGTCATTGATCTCAAGGCAACGGGAAATCGCTATATGTTTACTGCTAGCGATGGTGACATGAAGCTTAAAGCTATTCTTCCATCAAATCTGACTTCTGACGTCATTTCTGGAAACATTCAAAATTTGGGGCTTATTCGCATTCTTGACTACTCTCTCAATGATATTCCAAACAAGTCAGAAAAGTATTTAATCGTGACAAAATGTGAGGTGGTTTCTCCTGCTCTAGAAAAGGAGGTAAAAACTGAGGTTAAGACAGAGGAAGCAGGTACTATTTTGAAACCGAAGGTAGAGCTTGATGATAAGAGCACATTTAACAATGGATCTGGTATTATTCTGAAACCAAAGCAAGAATTGGTCGCTAAATCAGCTGCTCAGATTGTACACGAGCAGCGTATGAATATGGCACCAGCTGCCCGGATGTCCATGACAAGGCGAGTTCAGCCCCTTGTCTACTTAAACCCTTACCTGGGAGATTGGACCATCAAAGTTCGTGTTACTGATAAGGGAAACCTGCGTACATACAAAAATGCTCAAGGAGAAGGTTGTGTTTTCAATGTGGTGTTGACAGATGAAGATGGTACACAAATACAGGCAACGATGTTCAATGATGCTGCAAGGAAGTTCTATGAGAAGTTTGCCCTCGGCAAAGTCTATTATGTTTCCAGGGGAACTTTGAAAGTTGCCAATAAGAAATTCAAGACAGTTCAAAATGATTATGAAATGACTTTGAATGTGAATTCTCAAGTAGACGAGGTCAGCAATGAAGCTGCTTTTGTTCCTGAAACAAAATATAATTTTGTCAAGATTGATATGTTAGGTCCTCACGTGAATGGAAGAGATCTCGTTGATGTAATTGGAGTTGTTCAAAATGTTTCTTCTACAATGAGCATTCGAAGGAAACTCAACAATGAGACAGCTCCAAAACGTGATGTAACCATTGCTGATGAAACGAAGAAGACAGTTGTGATATCTTTGTGGAATGATCTTGCAACCACTGTGGGGCAGGAGTTGCTTGACATTGCCGACCAATCTCCTATAATTGCATTGAAATATCTTAGAGTTGGAGACTTTCAAGGTGTATCTTTGTCGACAATAAGCAGGAGCTCAATTACAATAAACCCAGACACAAAGGAAGCACATGATTTGAGATCATGGTATGACTCTGAAGGTAAAGCCGTATCCATGTCTTCTGTAAGCTCCGGCCTGAGTCCATCAACCAAGAGCGGTTCTAGGTCCATGTACTCTGACCGGATTTCCTTATCTCATATACTCGAAAACAAATCTTTGGGAGAAGAAAAGCCCACGTTCTTCAGCATTAGAGCATATGTCAGCTTTATCAAACCCGATCAAACAATGTGGTATCGTGCTTGCAAAACATGCAATAAGAAGGTAACTGAAGCCATCGGATCTGGATATTGGTGTGACAATTGCCAGAAAAACGATGAAGAATGCAGTTTAAGGTACATAATGGTTGTGAGGGTATCTGATGCAAGCGGTGAAGCTTGGGTCTCTACATTCAACGAAGAAGCAGAGAGAATTATGGGATGCTCAGCTGATGAACTTGATCAGCTCAAATCGCAGATTGGTGAGGAGAATTCATATCAACTTAAACTCAAAGAAGCCACATGGGTTCCCCACTTGTTTCGAGTTAGCGTTTCTCAGAACGAATACAACAATGAGAAGAGACAAAGGATCACCGTCAGGTCAGTTGCTCCAGTTGATTTTGCTGCTGAGTCCAGATTTTTGCTAGAAGAGATAGCAAAAATGAAGGCTTCCTAA

Coding sequence (CDS)

ATGGCGAGTGCACCAACTGCTGGCGGAATATCAAAGGTTTTGTCCAATCCATCACCGGACTCCCCCTCCGATGTCCCCGACGTTGTGGTTCAGGTCATTGATCTCAAGGCAACGGGAAATCGCTATATGTTTACTGCTAGCGATGGTGACATGAAGCTTAAAGCTATTCTTCCATCAAATCTGACTTCTGACGTCATTTCTGGAAACATTCAAAATTTGGGGCTTATTCGCATTCTTGACTACTCTCTCAATGATATTCCAAACAAGTCAGAAAAGTATTTAATCGTGACAAAATGTGAGGTGGTTTCTCCTGCTCTAGAAAAGGAGGTAAAAACTGAGGTTAAGACAGAGGAAGCAGGTACTATTTTGAAACCGAAGGTAGAGCTTGATGATAAGAGCACATTTAACAATGGATCTGGTATTATTCTGAAACCAAAGCAAGAATTGGTCGCTAAATCAGCTGCTCAGATTGTACACGAGCAGCGTATGAATATGGCACCAGCTGCCCGGATGTCCATGACAAGGCGAGTTCAGCCCCTTGTCTACTTAAACCCTTACCTGGGAGATTGGACCATCAAAGTTCGTGTTACTGATAAGGGAAACCTGCGTACATACAAAAATGCTCAAGGAGAAGGTTGTGTTTTCAATGTGGTGTTGACAGATGAAGATGGTACACAAATACAGGCAACGATGTTCAATGATGCTGCAAGGAAGTTCTATGAGAAGTTTGCCCTCGGCAAAGTCTATTATGTTTCCAGGGGAACTTTGAAAGTTGCCAATAAGAAATTCAAGACAGTTCAAAATGATTATGAAATGACTTTGAATGTGAATTCTCAAGTAGACGAGGTCAGCAATGAAGCTGCTTTTGTTCCTGAAACAAAATATAATTTTGTCAAGATTGATATGTTAGGTCCTCACGTGAATGGAAGAGATCTCGTTGATGTAATTGGAGTTGTTCAAAATGTTTCTTCTACAATGAGCATTCGAAGGAAACTCAACAATGAGACAGCTCCAAAACGTGATGTAACCATTGCTGATGAAACGAAGAAGACAGTTGTGATATCTTTGTGGAATGATCTTGCAACCACTGTGGGGCAGGAGTTGCTTGACATTGCCGACCAATCTCCTATAATTGCATTGAAATATCTTAGAGTTGGAGACTTTCAAGGTGTATCTTTGTCGACAATAAGCAGGAGCTCAATTACAATAAACCCAGACACAAAGGAAGCACATGATTTGAGATCATGGTATGACTCTGAAGGTAAAGCCGTATCCATGTCTTCTGTAAGCTCCGGCCTGAGTCCATCAACCAAGAGCGGTTCTAGGTCCATGTACTCTGACCGGATTTCCTTATCTCATATACTCGAAAACAAATCTTTGGGAGAAGAAAAGCCCACGTTCTTCAGCATTAGAGCATATGTCAGCTTTATCAAACCCGATCAAACAATGTGGTATCGTGCTTGCAAAACATGCAATAAGAAGGTAACTGAAGCCATCGGATCTGGATATTGGTGTGACAATTGCCAGAAAAACGATGAAGAATGCAGTTTAAGGTACATAATGGTTGTGAGGGTATCTGATGCAAGCGGTGAAGCTTGGGTCTCTACATTCAACGAAGAAGCAGAGAGAATTATGGGATGCTCAGCTGATGAACTTGATCAGCTCAAATCGCAGATTGGTGAGGAGAATTCATATCAACTTAAACTCAAAGAAGCCACATGGGTTCCCCACTTGTTTCGAGTTAGCGTTTCTCAGAACGAATACAACAATGAGAAGAGACAAAGGATCACCGTCAGGTCAGTTGCTCCAGTTGATTTTGCTGCTGAGTCCAGATTTTTGCTAGAAGAGATAGCAAAAATGAAGGCTTCCTAA

Protein sequence

MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSNLTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAGTILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPLVYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFYEKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKIDMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDLATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSEGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPDQTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRSVAPVDFAAESRFLLEEIAKMKAS*
Homology
BLAST of Chy2G047070 vs. ExPASy Swiss-Prot
Match: Q9SD82 (Replication protein A 70 kDa DNA-binding subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA1B PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 3.1e-246
Identity = 423/624 (67.79%), Postives = 516/624 (82.69%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           M ++ T  GI+ VL+N S DS S  P++VVQV+DLK  GNRY F+A+DG MK+KA+LP+ 
Sbjct: 1   MENSVTQDGIATVLANQSLDSSSVRPEIVVQVVDLKPAGNRYTFSANDGKMKIKAMLPAT 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKS-EKYLIVTKCEVVSPALEKEVKTEVKTEEA 120
           LTSD+ISG IQNLGLIR+L+Y++NDIP KS EKY+++TKCE V+ AL+ E+K E+K    
Sbjct: 61  LTSDIISGKIQNLGLIRLLEYTVNDIPGKSEEKYMLITKCEAVASALDSEIKAEIKA--- 120

Query: 121 GTILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQP 180
                              +GI+LKPK E VAKSA+QI++EQR N APAARM+MTRRV P
Sbjct: 121 ------------------STGIMLKPKHEFVAKSASQIINEQRGNAAPAARMAMTRRVHP 180

Query: 181 LVYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKF 240
           LV LNPY G WTIKVRVT+KG +RTYKNA+GEGCVFNV LTDE+GTQIQATMFN AARKF
Sbjct: 181 LVSLNPYQGSWTIKVRVTNKGVMRTYKNARGEGCVFNVELTDEEGTQIQATMFNAAARKF 240

Query: 241 YEKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVK 300
           Y++F +GKVYY+SRG+LK+ANK+FKTVQNDYEMTLN NS+V+E SNE  F PETK+NFV 
Sbjct: 241 YDRFEMGKVYYISRGSLKLANKQFKTVQNDYEMTLNENSEVEEASNEEMFTPETKFNFVP 300

Query: 301 IDMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWND 360
           ID LG +VN +DL+DVIGVVQ+VS TMSIRRK +NE  PKRD+T+ADETKKTVV+SLWND
Sbjct: 301 IDELGTYVNQKDLIDVIGVVQSVSPTMSIRRKNDNEMIPKRDITLADETKKTVVVSLWND 360

Query: 361 LATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDS 420
           LAT +GQELLD+AD  P+IA+K L+VG FQGVSLSTISRS++ INP++ EA  L+SWYD+
Sbjct: 361 LATGIGQELLDMADNHPVIAIKSLKVGAFQGVSLSTISRSNVVINPNSPEATKLKSWYDA 420

Query: 421 EGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKP 480
           EGK  SMS++ SG+S S  +GSRSMYSDR+ LSHI  N SLGEEKP FFS RAY+SFIKP
Sbjct: 421 EGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEEKPVFFSTRAYISFIKP 480

Query: 481 DQTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNE 540
           DQTMWYRACKTCNKKVTEA+ SGYWC++CQK D+ECSLRYIM V+VSD++GE W+S FN+
Sbjct: 481 DQTMWYRACKTCNKKVTEAMDSGYWCESCQKKDQECSLRYIMAVKVSDSTGETWLSAFND 540

Query: 541 EAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVR 600
           EAE+I+GC+AD+L+ LKS+ GE N +Q KLKEATW  HLFR+SVSQ EYN+EKRQRITVR
Sbjct: 541 EAEKIIGCTADDLNDLKSEEGEVNEFQTKLKEATWSSHLFRISVSQQEYNSEKRQRITVR 600

Query: 601 SVAPVDFAAESRFLLEEIAKMKAS 624
            V+P+DFAAE+R LL++I+K K S
Sbjct: 601 GVSPIDFAAETRLLLQDISKNKTS 603

BLAST of Chy2G047070 vs. ExPASy Swiss-Prot
Match: Q9FME0 (Replication protein A 70 kDa DNA-binding subunit D OS=Arabidopsis thaliana OX=3702 GN=RPA1D PE=2 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 3.2e-243
Identity = 417/622 (67.04%), Postives = 521/622 (83.76%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           M ++ T   IS VLSNPS DS SD  ++VVQV+DLK  GNRY F+A+DG  K+KA+  ++
Sbjct: 1   MQTSVTPDAISTVLSNPSFDSSSDRSEIVVQVVDLKPIGNRYTFSANDGKTKVKAMFTAS 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKT--EE 120
           LT ++ISG IQNLGLIR++D+++NDI +KS KY +VTKCE V   L+ E+  + K+  EE
Sbjct: 61  LTPEIISGKIQNLGLIRLIDFTVNDISSKSTKYFLVTKCEAVGSVLDSEINLDSKSGEEE 120

Query: 121 AGTILKPKVELDDKSTFNN--GSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRR 180
           A    K K+E    S  N+   +GI LKPKQE VAKSA+QI+ EQR N APAARM+MTRR
Sbjct: 121 AREPKKQKLEHSPVSPLNDVVSTGITLKPKQEFVAKSASQIMSEQRGNAAPAARMAMTRR 180

Query: 181 VQPLVYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAA 240
           V PLV LNPY G+WTIKVRVT+KG +R YKNA+GEGCVFNV LTDE+GTQIQATMFNDAA
Sbjct: 181 VHPLVSLNPYQGNWTIKVRVTNKGVMRNYKNARGEGCVFNVELTDEEGTQIQATMFNDAA 240

Query: 241 RKFYEKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYN 300
           RKF+++F LGKVYY+SRG+LK+ANK+FKTVQNDYEMTLN NS+V+E S+E  F+PETK+N
Sbjct: 241 RKFFDRFQLGKVYYISRGSLKLANKQFKTVQNDYEMTLNENSEVEEASSEEMFIPETKFN 300

Query: 301 FVKIDMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISL 360
           FV I+ LG +VN ++L+D+IGVVQ+VS TMSIRR+ +NE  PKRD+T+ADE++KTVV+SL
Sbjct: 301 FVPIEELGLYVNQKELIDLIGVVQSVSPTMSIRRRTDNEMIPKRDITLADESRKTVVVSL 360

Query: 361 WNDLATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSW 420
           WNDLAT +GQELLD+ADQSP+IA+K L+VGDFQGVSLSTISRS++ INP++ EA  L+SW
Sbjct: 361 WNDLATGIGQELLDMADQSPVIAIKSLKVGDFQGVSLSTISRSNVVINPESPEAKKLKSW 420

Query: 421 YDSEGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSF 480
           +DSEGK +SMSS+ SG+SPS K+GSRS+Y+DR+ LSHI  N SL EEKP FFS RAY+SF
Sbjct: 421 FDSEGKEISMSSIGSGMSPSAKNGSRSLYTDRVLLSHITSNPSLFEEKPVFFSTRAYISF 480

Query: 481 IKPDQTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVST 540
           IKPDQTMWY+ACKTCNKKVTEA+ SGYWC+ CQ+  EECSLRYIM V+V+D+SGE W+S+
Sbjct: 481 IKPDQTMWYQACKTCNKKVTEALDSGYWCEGCQRKYEECSLRYIMAVKVTDSSGETWISS 540

Query: 541 FNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRI 600
           FN+EAE+I+GCSADEL++LKS+ GE N YQ KLKEATW  H+FRVSV+QNEYN EKRQR+
Sbjct: 541 FNDEAEKILGCSADELNKLKSEEGEVNEYQTKLKEATWSSHVFRVSVTQNEYNGEKRQRV 600

Query: 601 TVRSVAPVDFAAESRFLLEEIA 619
           TV+ VAP+DFAAE+R LL++I+
Sbjct: 601 TVKGVAPLDFAAETRLLLQDIS 622

BLAST of Chy2G047070 vs. ExPASy Swiss-Prot
Match: Q10Q08 (Replication protein A 70 kDa DNA-binding subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA1B PE=1 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 4.1e-230
Identity = 405/626 (64.70%), Postives = 501/626 (80.03%), Query Frame = 0

Query: 2   ASAPTAGGISKVLSNPSPDSPS--DVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPS 61
           A + T G ++ VL N SPD+ +   VP++V+QV+DLK  G R+ F ASDG  K+K +L +
Sbjct: 6   APSVTPGAVAFVLENASPDAATGVPVPEIVLQVVDLKPIGTRFTFLASDGKDKIKTMLLT 65

Query: 62  NLTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEE- 121
            L  +V SGNIQNLG+IR+LDY+ N I  K EK LI+TK EVV  AL+ E+K E + +E 
Sbjct: 66  QLAPEVRSGNIQNLGVIRVLDYTCNTIGEKQEKVLIITKLEVVFKALDSEIKCEAEKQEE 125

Query: 122 -AGTILKPKVELDDKSTFNNG---SGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMT 181
               +L PK E    S   N      ++LKPKQE+  KSA+QIV+EQR N APAAR++MT
Sbjct: 126 KPAILLSPKEESVVLSKPTNAPPLPPVVLKPKQEV--KSASQIVNEQRGNAAPAARLAMT 185

Query: 182 RRVQPLVYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFND 241
           RRV PL+ LNPY G+W IKVRVT KGNLRTYKNA+GEGCVFNV LTD DGTQIQATMFN+
Sbjct: 186 RRVHPLISLNPYQGNWIIKVRVTSKGNLRTYKNARGEGCVFNVELTDVDGTQIQATMFNE 245

Query: 242 AARKFYEKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETK 301
           AA+KFY  F LGKVYY+S+G+L+VANK+FKTV NDYEMTLN N+ V+E   E  F+P+ +
Sbjct: 246 AAKKFYPMFELGKVYYISKGSLRVANKQFKTVHNDYEMTLNENAVVEEAEGE-TFIPQIQ 305

Query: 302 YNFVKIDMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVI 361
           YNFVKID LGP+V GR+LVDVIGVVQ+VS T+S+RRK++NET PKRD+ +AD++ KTV I
Sbjct: 306 YNFVKIDQLGPYVGGRELVDVIGVVQSVSPTLSVRRKIDNETIPKRDIVVADDSSKTVTI 365

Query: 362 SLWNDLATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLR 421
           SLWNDLATT GQELLD+ D +PIIA+K L+V DFQG+SLST+ RS+I +NPD  EA  LR
Sbjct: 366 SLWNDLATTTGQELLDMVDSAPIIAIKSLKVSDFQGLSLSTVGRSTIVVNPDLPEAEQLR 425

Query: 422 SWYDSEGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYV 481
           +WYDSEGK  SM+S+ S +  S   G+RSMYSDR+ LSHI  + +LG++KP FFS+ AY+
Sbjct: 426 AWYDSEGKGTSMASIGSDMGASRVGGARSMYSDRVFLSHITSDPNLGQDKPVFFSLNAYI 485

Query: 482 SFIKPDQTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWV 541
           S IKPDQTMWYRACKTCNKKVTEAIGSGYWC+ CQKND ECSLRYIMV++VSD +GEAW+
Sbjct: 486 SLIKPDQTMWYRACKTCNKKVTEAIGSGYWCEGCQKNDAECSLRYIMVIKVSDPTGEAWL 545

Query: 542 STFNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQ 601
           S FN++AERI+GCSADELD+++ + G++ SY LKLKEATWVPHLFRVSV+QNEY NEKRQ
Sbjct: 546 SLFNDQAERIVGCSADELDRIRKEEGDD-SYLLKLKEATWVPHLFRVSVTQNEYMNEKRQ 605

Query: 602 RITVRSVAPVDFAAESRFLLEEIAKM 621
           RITVRS APVD AAE++++LEEIAK+
Sbjct: 606 RITVRSEAPVDHAAEAKYMLEEIAKL 627

BLAST of Chy2G047070 vs. ExPASy Swiss-Prot
Match: Q9SKI4 (Replication protein A 70 kDa DNA-binding subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA1A PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.8e-82
Identity = 194/627 (30.94%), Postives = 331/627 (52.79%), Query Frame = 0

Query: 29  VVQVIDLKATG-------NRYMFTASDGDMKLKAILPSNLTSDVISGNIQNLGLIRILDY 88
           ++QV+++K  G        RY F  SDG     A++   L   V SG  +   +++++DY
Sbjct: 23  LLQVLEIKMIGRSQERSQERYRFLISDGVSAQHAMVAVQLNDRVKSGQFEKGSIVQLIDY 82

Query: 89  SLNDIPNKSEKYLIVTKCEVV--------SPALEKEVKTEVKTEEAGT----------IL 148
             +D+  K  K ++V   E +        +P +  E  TE +   +GT            
Sbjct: 83  ICSDV--KGRKLIVVLNMETIVQQSETIGNPTIFGETDTEAQKTFSGTGNIPPPNRVVFN 142

Query: 149 KPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHE---QRMNMAPAARMSMTRRVQPL 208
           +P V+         G  I  +       + + Q  ++      N  P  +     RV P+
Sbjct: 143 EPMVQHSVNRAPPRGVNIQNQANNTPSFRPSVQPSYQPPASYRNHGPIMKNEAPARVIPI 202

Query: 209 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 268
             LNPY G W IK RVT KG++R Y NA+G+G VF+  L D DG +I+ T FN    +FY
Sbjct: 203 AALNPYQGRWAIKARVTAKGDIRRYNNAKGDGKVFSFDLLDYDGGEIRVTCFNALVDRFY 262

Query: 269 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 328
           +   +GKVY +S+G+LK A K F  ++N++E+ L   S V+   +E   +P+ +++F  I
Sbjct: 263 DVTEVGKVYLISKGSLKPAQKNFNHLKNEWEIFLESTSTVELCPDEDGSIPKQQFSFRPI 322

Query: 329 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 388
             +  +     ++DVIGVV +V+ ++ I RK   ET  +R + + DE+ K V ++LW + 
Sbjct: 323 SDI-ENAENNTILDVIGVVTSVNPSVPILRKNGMET-HRRILNLKDESGKAVEVTLWGEF 382

Query: 389 ATTVGQELLDIADQS--PIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYD 448
               G++L ++ D +  P++A+K  +V DF G S+ TIS + + INPD  EAH LR+W+D
Sbjct: 383 CNRDGRQLEEMVDSAFHPVLAIKAGKVSDFSGKSVGTISSTQLFINPDFPEAHKLRTWFD 442

Query: 449 SEGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIK 508
             GK     + S  +S  T  G  S    R ++S I E      +KP + +++A +SFIK
Sbjct: 443 YGGK----DTASFSISRDTMPGGVSRNEIRKNVSQIKEEGLGRSDKPDWITVKATISFIK 502

Query: 509 PDQTMWYRAC------KTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEA 568
            D +  Y AC      K CNKKVT +  + + CD C +  +EC  RY++ V++ D +G  
Sbjct: 503 TD-SFCYTACPLMIGDKQCNKKVTRSGTNRWLCDRCNQESDECDYRYLLQVQIQDHTGLT 562

Query: 569 WVSTFNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEK 620
           W++ F E  E IMGC A +L  +K ++ +E  +   +++  +  ++ ++ + +  Y +E+
Sbjct: 563 WITAFQETGEEIMGCPAKKLYAMKYELEKEEEFAEIVRDRLFHQYMLKLKIKEESYGDEQ 622

BLAST of Chy2G047070 vs. ExPASy Swiss-Prot
Match: Q6YZ49 (Replication protein A 70 kDa DNA-binding subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA1A PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 7.8e-80
Identity = 199/665 (29.92%), Postives = 342/665 (51.43%), Query Frame = 0

Query: 6   TAGGISKVLSNPSPDSPSDVPDVVVQVIDLKAT---------GNRYMFTASDGDMKLKAI 65
           T  G++  L+  +   P      V+Q+++L+           G R+    SDG     A+
Sbjct: 7   TPNGVAAALAGDTNLKP------VLQIVELRGVQVNGAGVTRGERFRAVVSDGTAASSAL 66

Query: 66  LPSNLTSDVISGNIQNLGLIRILDYSLNDIPNKS-----EKYLIVTKCEVV-SPALEKEV 125
             + L+    SG ++   ++++ +Y +N++  +         ++V++CE++ +P    E 
Sbjct: 67  FAAQLSDHARSGALRRGSIVQLSEYVINEVGPRRIIVILNLEVLVSECEIIGNPTALSET 126

Query: 126 KTEVKT------------------EEAGTILKP--KVELDDKSTFNNGSGIILKPKQEL- 185
            + +                      + T  KP   V L   S   N S    +P  ++ 
Sbjct: 127 GSPIPNPTRVEQFNGAPQYGLMAGNSSNTTTKPSDNVPLFQNSMAGNSSNFATRPSDKVP 186

Query: 186 VAKSAAQIVHE---QRMNMAPAARMSMTRRVQPLVYLNPYLGDWTIKVRVTDKGNLRTYK 245
           V +   Q  +       N     +     R+ P+  LNPY G W IK RVT KG++R Y 
Sbjct: 187 VFQPTVQPSYRPAPNYKNHGAIMKNEAPARIIPISALNPYQGRWAIKARVTAKGDIRRYH 246

Query: 246 NAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFYEKFALGKVYYVSRGTLKVANKKFKTV 305
           NA+G+G VF+  L D DG +I+ T FN    +FYE   +GKVY VSRG L+ A K +  +
Sbjct: 247 NAKGDGKVFSFDLLDSDGGEIRVTCFNALLDRFYEVVEVGKVYVVSRGNLRPAQKNYNHL 306

Query: 306 QNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKIDMLGPHVNGRDLVDVIGVVQNVSSTM 365
            N++E+ L   S VD   +E + +P  +++F  I+ +    N   ++D+IGVV +V+   
Sbjct: 307 NNEWEILLENGSTVDLCPDENSSIPTQRFDFRPINEIEDAQNNA-ILDIIGVVTSVNPCT 366

Query: 366 SIRRKLNNETAPKRDVTIADETKKTVVISLWNDLATTVGQELLDIADQS--PIIALKYLR 425
           +I+RK   ET  KR + + D + ++V +++W D     G +L  + ++   P++A+K  +
Sbjct: 367 TIQRKNGMET-QKRTMNLKDMSGRSVEVTMWGDFCNREGSQLQGMVERGIFPVLAVKAGK 426

Query: 426 VGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSEGKAVSMSSVSSGLSPSTKSGSRSM 485
           V DF G S+ TIS + + INPD+ EAH LR W+DS G+  S  S+S  ++P     SR+ 
Sbjct: 427 VSDFSGKSVGTISSTQLFINPDSAEAHSLRQWFDSGGRDASTQSISRDITP---GASRNE 486

Query: 486 YSDRISLSHILENKSLG-EEKPTFFSIRAYVSFIKPDQTMWYRAC------KTCNKKVTE 545
               ++    ++++ LG  +KP + +++A V F K +++ +Y AC      + CNKKVT+
Sbjct: 487 IRKTVAQ---IKDEGLGMGDKPDWITVKATVIFFK-NESFFYTACPNMIGDRQCNKKVTK 546

Query: 546 AIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEEAERIMGCSADELDQLKS 605
           +    + CD C +  EEC  RY++  ++ D SG AWV+ F E  + ++GCSA EL+ LK 
Sbjct: 547 STNGNWTCDKCDREFEECDYRYLLQFQIQDHSGTAWVTAFQEAGQELLGCSATELNALKE 606

Query: 606 QIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRSVAPVDFAAESRFLLEEI 623
           +  E+  +   +    +  +L R+ V +  Y +E++ + T   V  VD + ES+FLL+ I
Sbjct: 607 R--EDPRFADTMLNCLFQEYLLRLKVKEESYGDERKVKNTAVKVEKVDPSGESKFLLDLI 654

BLAST of Chy2G047070 vs. ExPASy TrEMBL
Match: A0A0A0LSG2 (Replication protein A subunit OS=Cucumis sativus OX=3659 GN=Csa_1G013800 PE=3 SV=1)

HSP 1 Score: 1204.9 bits (3116), Expect = 0.0e+00
Identity = 619/623 (99.36%), Postives = 621/623 (99.68%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDG MKLKAILPSN
Sbjct: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGVMKLKAILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG
Sbjct: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
           TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL
Sbjct: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY
Sbjct: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRK+NNETAPKRDVTIADETKKTVVISLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKINNETAPKRDVTIADETKKTVVISLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPD KEAHDLRSWYDSE
Sbjct: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDIKEAHDLRSWYDSE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD
Sbjct: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE
Sbjct: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 624
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. ExPASy TrEMBL
Match: A0A5A7UTE7 (Replication protein A subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003010 PE=3 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 600/623 (96.31%), Postives = 614/623 (98.56%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MASAPTAGGISK+LSNPSPDSP+DVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN
Sbjct: 1   MASAPTAGGISKILSNPSPDSPTDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTSDVISGNIQNLGLIRILDYSLNDIPNKS+KYLIV KC VVSPALEKEVKTEVK EEAG
Sbjct: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSDKYLIVAKCVVVSPALEKEVKTEVKKEEAG 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            +LKPKVELDDKS FNNGSGI+LKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL
Sbjct: 121 IVLKPKVELDDKSKFNNGSGILLKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY
Sbjct: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVS+EAAFVPETKYNFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSSEAAFVPETKYNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRK+NNETAPKRDVTIADETKKTVVISLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKINNETAPKRDVTIADETKKTVVISLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDIADQSPIIA+KYLRVGDFQGVSLSTISRSSITINPD KEAHDLRSWYD+E
Sbjct: 361 ATTVGQELLDIADQSPIIAVKYLRVGDFQGVSLSTISRSSITINPDIKEAHDLRSWYDAE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKAVSMSSVSSG+SPSTKSGSRSMYSDR+SLSHI +NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAVSMSSVSSGMSPSTKSGSRSMYSDRVSLSHINQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGE WVSTFNEE
Sbjct: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEVWVSTFNEE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIIGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 624
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. ExPASy TrEMBL
Match: A0A1S3BX02 (Replication protein A subunit OS=Cucumis melo OX=3656 GN=LOC103494045 PE=3 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 600/623 (96.31%), Postives = 614/623 (98.56%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MASAPTAGGISK+LSNPSPDSP+DVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN
Sbjct: 1   MASAPTAGGISKILSNPSPDSPTDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTSDVISGNIQNLGLIRILDYSLNDIPNKS+KYLIV KC VVSPALEKEVKTEVK EEAG
Sbjct: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSDKYLIVAKCVVVSPALEKEVKTEVKKEEAG 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            +LKPKVELDDKS FNNGSGI+LKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL
Sbjct: 121 IVLKPKVELDDKSKFNNGSGILLKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY
Sbjct: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVS+EAAFVPETKYNFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSSEAAFVPETKYNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRK+NNETAPKRDVTIADETKKTVVISLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKINNETAPKRDVTIADETKKTVVISLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDIADQSPIIA+KYLRVGDFQGVSLSTISRSSITINPD KEAHDLRSWYD+E
Sbjct: 361 ATTVGQELLDIADQSPIIAVKYLRVGDFQGVSLSTISRSSITINPDIKEAHDLRSWYDAE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKAVSMSSVSSG+SPSTKSGSRSMYSDR+SLSHI +NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAVSMSSVSSGMSPSTKSGSRSMYSDRVSLSHINQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGE WVSTFNEE
Sbjct: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEVWVSTFNEE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIIGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 624
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. ExPASy TrEMBL
Match: A0A6J1F085 (Replication protein A subunit OS=Cucurbita moschata OX=3662 GN=LOC111440985 PE=3 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 562/623 (90.21%), Postives = 596/623 (95.67%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MA+APT GGISK+L+NPSPDS SDVP VV+QV+DLKATGNRYMFTA+DGDMKLK+ILPSN
Sbjct: 1   MANAPTPGGISKILANPSPDSASDVPQVVLQVLDLKATGNRYMFTAADGDMKLKSILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTS+VISGNIQNLGLIR+LDYSLNDIPNK EKYLIVTKCEVVS ALEKEVK EVK EEA 
Sbjct: 61  LTSEVISGNIQNLGLIRVLDYSLNDIPNKPEKYLIVTKCEVVSSALEKEVKFEVKKEEAD 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            +LKPK ELDDKS  N GSGI+LKPKQELVAKSAAQIVHEQ  NMAP+ARMSMTRRV PL
Sbjct: 121 IVLKPKQELDDKSAVNTGSGILLKPKQELVAKSAAQIVHEQSTNMAPSARMSMTRRVHPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPY GDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFN+AARKFY
Sbjct: 181 VYLNPYQGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNEAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEV+NEAAFVPETK+NFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVTNEAAFVPETKFNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDV+GVVQ+VS TMSIRRK+NNETAPKRDVTIADETKKTVV+SLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVVGVVQSVSPTMSIRRKINNETAPKRDVTIADETKKTVVVSLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDI DQSPIIALKYLRVGDFQGVSLSTISRSSI INPD KEAH LRSWYDSE
Sbjct: 361 ATTVGQELLDIVDQSPIIALKYLRVGDFQGVSLSTISRSSILINPDIKEAHQLRSWYDSE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKA+SM+SVSSG+SPS+KSGSRSMY+DR+SLSHIL+NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAISMASVSSGMSPSSKSGSRSMYTDRVSLSHILQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVT+AIGSGYWC++CQKNDEECSLRYIMVVRVSDAS EAW+STFN E
Sbjct: 481 QTMWYRACKTCNKKVTDAIGSGYWCESCQKNDEECSLRYIMVVRVSDASCEAWLSTFNAE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GC+A+ELD LKSQIGEE SYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIVGCTAEELDGLKSQIGEEASYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 624
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. ExPASy TrEMBL
Match: A0A6J1I6K6 (Replication protein A subunit OS=Cucurbita maxima OX=3661 GN=LOC111470404 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 561/623 (90.05%), Postives = 597/623 (95.83%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MA+APT GGISK+L+NPSPDS SDVP VV+QV+DLKATGNRYMFTA+DGDMKLK+ILPSN
Sbjct: 1   MANAPTPGGISKILANPSPDSASDVPQVVLQVLDLKATGNRYMFTAADGDMKLKSILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTS+VISGNIQNLGLIR+LDYSLNDIPNK EKYLIVTKCEVVS AL+KEVK+EVK EEA 
Sbjct: 61  LTSEVISGNIQNLGLIRVLDYSLNDIPNKPEKYLIVTKCEVVSSALDKEVKSEVKKEEAD 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            +LKPK ELDDKS  N GSGI+LKPKQELVAKSAAQIVHEQR NMAP+ARMSMTRRV PL
Sbjct: 121 IVLKPKQELDDKSAVNTGSGILLKPKQELVAKSAAQIVHEQRTNMAPSARMSMTRRVHPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPY GDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVL DEDGTQIQATMFN+AARKFY
Sbjct: 181 VYLNPYQGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLADEDGTQIQATMFNEAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEV+NEAAFVPETK+NFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVTNEAAFVPETKFNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGR+LVDVIGVVQ+VS TMSIRRK+NNETAPKRDVTIADETKKTVV+SLWNDL
Sbjct: 301 DMLGPHVNGRELVDVIGVVQSVSPTMSIRRKINNETAPKRDVTIADETKKTVVVSLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDI DQSPIIALKYLRVGDFQGVSLSTISRSSI INPD KEAH LRSWYDSE
Sbjct: 361 ATTVGQELLDIVDQSPIIALKYLRVGDFQGVSLSTISRSSILINPDIKEAHQLRSWYDSE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKA+SM+SVSSG+SPS+KSGSRSMY+DR+SLSHIL+NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAISMASVSSGMSPSSKSGSRSMYTDRVSLSHILQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVT+AIGSGYWC++CQKNDEECSLRYIMVVRVSDAS EAW+STFN E
Sbjct: 481 QTMWYRACKTCNKKVTDAIGSGYWCESCQKNDEECSLRYIMVVRVSDASCEAWLSTFNAE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GC+A+ELD LKSQIGEE SYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIVGCTAEELDGLKSQIGEEASYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 624
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. NCBI nr
Match: XP_011648462.1 (replication protein A 70 kDa DNA-binding subunit B [Cucumis sativus] >KGN63732.1 hypothetical protein Csa_014362 [Cucumis sativus])

HSP 1 Score: 1203 bits (3113), Expect = 0.0
Identity = 619/623 (99.36%), Postives = 621/623 (99.68%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDG MKLKAILPSN
Sbjct: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGVMKLKAILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG
Sbjct: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
           TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL
Sbjct: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY
Sbjct: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRK+NNETAPKRDVTIADETKKTVVISLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKINNETAPKRDVTIADETKKTVVISLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPD KEAHDLRSWYDSE
Sbjct: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDIKEAHDLRSWYDSE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD
Sbjct: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE
Sbjct: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 623
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. NCBI nr
Match: XP_008453278.1 (PREDICTED: replication protein A 70 kDa DNA-binding subunit B [Cucumis melo] >KAA0057997.1 replication protein A 70 kDa DNA-binding subunit B [Cucumis melo var. makuwa])

HSP 1 Score: 1177 bits (3044), Expect = 0.0
Identity = 600/623 (96.31%), Postives = 614/623 (98.56%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MASAPTAGGISK+LSNPSPDSP+DVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN
Sbjct: 1   MASAPTAGGISKILSNPSPDSPTDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTSDVISGNIQNLGLIRILDYSLNDIPNKS+KYLIV KC VVSPALEKEVKTEVK EEAG
Sbjct: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSDKYLIVAKCVVVSPALEKEVKTEVKKEEAG 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            +LKPKVELDDKS FNNGSGI+LKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL
Sbjct: 121 IVLKPKVELDDKSKFNNGSGILLKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY
Sbjct: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVS+EAAFVPETKYNFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSSEAAFVPETKYNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRK+NNETAPKRDVTIADETKKTVVISLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKINNETAPKRDVTIADETKKTVVISLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDIADQSPIIA+KYLRVGDFQGVSLSTISRSSITINPD KEAHDLRSWYD+E
Sbjct: 361 ATTVGQELLDIADQSPIIAVKYLRVGDFQGVSLSTISRSSITINPDIKEAHDLRSWYDAE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKAVSMSSVSSG+SPSTKSGSRSMYSDR+SLSHI +NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAVSMSSVSSGMSPSTKSGSRSMYSDRVSLSHINQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGE WVSTFNEE
Sbjct: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEVWVSTFNEE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIIGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 623
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. NCBI nr
Match: XP_038878718.1 (replication protein A 70 kDa DNA-binding subunit B-like [Benincasa hispida])

HSP 1 Score: 1151 bits (2978), Expect = 0.0
Identity = 587/623 (94.22%), Postives = 604/623 (96.95%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MA APT GGIS +LSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN
Sbjct: 1   MADAPTVGGISNILSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTSDVISGNIQNLGLIR+LDYSLNDIPNK EKYLIVTKCEVVSPALEKEVKTEVK EE G
Sbjct: 61  LTSDVISGNIQNLGLIRVLDYSLNDIPNKQEKYLIVTKCEVVSPALEKEVKTEVKKEETG 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            ILKPK+E DDKSTFNNGSGI+LKPKQELVAKSAAQIVHEQ+ NMAPAARMSMTRRV PL
Sbjct: 121 IILKPKLEDDDKSTFNNGSGILLKPKQELVAKSAAQIVHEQQTNMAPAARMSMTRRVHPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPY GDWTIKVRVTDKGNLRTYKNAQGEG VFNVVLTDEDGTQIQATMFNDAARKFY
Sbjct: 181 VYLNPYQGDWTIKVRVTDKGNLRTYKNAQGEGYVFNVVLTDEDGTQIQATMFNDAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYY+SRG LKVANKKFKTVQNDYEMTLNVNSQVDEVS+EAAFVPETKYNFVKI
Sbjct: 241 EKFSLGKVYYISRGALKVANKKFKTVQNDYEMTLNVNSQVDEVSSEAAFVPETKYNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDVIGVVQ+VS TMSIRRK+NNETAPKRDVTIADETKKTVVISLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVIGVVQSVSPTMSIRRKINNETAPKRDVTIADETKKTVVISLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDIADQSPIIA+KYLRVGDFQGVSLSTISRSSI INPD KEAHDLRSWYDSE
Sbjct: 361 ATTVGQELLDIADQSPIIAVKYLRVGDFQGVSLSTISRSSIMINPDIKEAHDLRSWYDSE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKAVSMSSVSSGLSPSTKSG+RSMYSDRISLSHIL+NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAVSMSSVSSGLSPSTKSGTRSMYSDRISLSHILQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTC KKVTEAIGSGYWCDNCQKNDEECSL+YIMVVRVSDASGEAW STFNEE
Sbjct: 481 QTMWYRACKTCKKKVTEAIGSGYWCDNCQKNDEECSLKYIMVVRVSDASGEAWFSTFNEE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GCSADELDQLKSQ+GEENSYQLKLK+ATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIIGCSADELDQLKSQVGEENSYQLKLKQATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 623
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. NCBI nr
Match: KAG6587586.1 (Replication protein A 70 kDa DNA-binding subunit B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021553.1 Replication protein A 70 kDa DNA-binding subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1112 bits (2875), Expect = 0.0
Identity = 564/623 (90.53%), Postives = 597/623 (95.83%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MA+APT GGISK+L+NPSPDS SDVP VV+QV+DLKATGNRYMFTA+DGDMKLK+ILPSN
Sbjct: 1   MANAPTPGGISKILANPSPDSASDVPQVVLQVLDLKATGNRYMFTAADGDMKLKSILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTS+VISGNIQNLGLIR+LDYSLNDIPNK EKYLIVTKCEVVS ALEKEVK EVK EEA 
Sbjct: 61  LTSEVISGNIQNLGLIRVLDYSLNDIPNKPEKYLIVTKCEVVSSALEKEVKFEVKKEEAD 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            +LKPK ELDDKS  N GSGI+LKPKQELVAKSAAQIVHEQ  NMAP+ARMSMTRRV PL
Sbjct: 121 IVLKPKQELDDKSAVNTGSGILLKPKQELVAKSAAQIVHEQSTNMAPSARMSMTRRVHPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPY GDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFN+AARKFY
Sbjct: 181 VYLNPYQGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNEAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEV+NEAAFVPETK+NFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVTNEAAFVPETKFNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDVIGVVQ+VS TMSIRRK+NNETAPKRDVTIADETKKTVV+SLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVIGVVQSVSPTMSIRRKINNETAPKRDVTIADETKKTVVVSLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSI INPD KEAH LRSWYDSE
Sbjct: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSILINPDIKEAHQLRSWYDSE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKA+SM+SVSSG+SPS+KSGSRSMY+DR+SLSHIL+NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAISMASVSSGMSPSSKSGSRSMYTDRVSLSHILQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVT+AIGSGYWC++CQKNDEECSLRYIMVVRVSDAS EAW+STFN E
Sbjct: 481 QTMWYRACKTCNKKVTDAIGSGYWCESCQKNDEECSLRYIMVVRVSDASCEAWLSTFNAE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GC+A+ELD LKSQIGEE SYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIVGCTAEELDGLKSQIGEEASYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 623
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. NCBI nr
Match: XP_022933609.1 (replication protein A 70 kDa DNA-binding subunit B-like [Cucurbita moschata])

HSP 1 Score: 1110 bits (2870), Expect = 0.0
Identity = 562/623 (90.21%), Postives = 596/623 (95.67%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           MA+APT GGISK+L+NPSPDS SDVP VV+QV+DLKATGNRYMFTA+DGDMKLK+ILPSN
Sbjct: 1   MANAPTPGGISKILANPSPDSASDVPQVVLQVLDLKATGNRYMFTAADGDMKLKSILPSN 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKTEEAG 120
           LTS+VISGNIQNLGLIR+LDYSLNDIPNK EKYLIVTKCEVVS ALEKEVK EVK EEA 
Sbjct: 61  LTSEVISGNIQNLGLIRVLDYSLNDIPNKPEKYLIVTKCEVVSSALEKEVKFEVKKEEAD 120

Query: 121 TILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQPL 180
            +LKPK ELDDKS  N GSGI+LKPKQELVAKSAAQIVHEQ  NMAP+ARMSMTRRV PL
Sbjct: 121 IVLKPKQELDDKSAVNTGSGILLKPKQELVAKSAAQIVHEQSTNMAPSARMSMTRRVHPL 180

Query: 181 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 240
           VYLNPY GDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFN+AARKFY
Sbjct: 181 VYLNPYQGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNEAARKFY 240

Query: 241 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 300
           EKF+LGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEV+NEAAFVPETK+NFVKI
Sbjct: 241 EKFSLGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVTNEAAFVPETKFNFVKI 300

Query: 301 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 360
           DMLGPHVNGRDLVDV+GVVQ+VS TMSIRRK+NNETAPKRDVTIADETKKTVV+SLWNDL
Sbjct: 301 DMLGPHVNGRDLVDVVGVVQSVSPTMSIRRKINNETAPKRDVTIADETKKTVVVSLWNDL 360

Query: 361 ATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSE 420
           ATTVGQELLDI DQSPIIALKYLRVGDFQGVSLSTISRSSI INPD KEAH LRSWYDSE
Sbjct: 361 ATTVGQELLDIVDQSPIIALKYLRVGDFQGVSLSTISRSSILINPDIKEAHQLRSWYDSE 420

Query: 421 GKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPD 480
           GKA+SM+SVSSG+SPS+KSGSRSMY+DR+SLSHIL+NKSLGEEKP FFSIRAYVSFIKPD
Sbjct: 421 GKAISMASVSSGMSPSSKSGSRSMYTDRVSLSHILQNKSLGEEKPMFFSIRAYVSFIKPD 480

Query: 481 QTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNEE 540
           QTMWYRACKTCNKKVT+AIGSGYWC++CQKNDEECSLRYIMVVRVSDAS EAW+STFN E
Sbjct: 481 QTMWYRACKTCNKKVTDAIGSGYWCESCQKNDEECSLRYIMVVRVSDASCEAWLSTFNAE 540

Query: 541 AERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600
           AERI+GC+A+ELD LKSQIGEE SYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS
Sbjct: 541 AERIVGCTAEELDGLKSQIGEEASYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVRS 600

Query: 601 VAPVDFAAESRFLLEEIAKMKAS 623
           VAPVDFAAESRFLLEEIAKMKAS
Sbjct: 601 VAPVDFAAESRFLLEEIAKMKAS 623

BLAST of Chy2G047070 vs. TAIR 10
Match: AT5G08020.1 (RPA70-kDa subunit B )

HSP 1 Score: 852.4 bits (2201), Expect = 2.2e-247
Identity = 423/624 (67.79%), Postives = 516/624 (82.69%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           M ++ T  GI+ VL+N S DS S  P++VVQV+DLK  GNRY F+A+DG MK+KA+LP+ 
Sbjct: 1   MENSVTQDGIATVLANQSLDSSSVRPEIVVQVVDLKPAGNRYTFSANDGKMKIKAMLPAT 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKS-EKYLIVTKCEVVSPALEKEVKTEVKTEEA 120
           LTSD+ISG IQNLGLIR+L+Y++NDIP KS EKY+++TKCE V+ AL+ E+K E+K    
Sbjct: 61  LTSDIISGKIQNLGLIRLLEYTVNDIPGKSEEKYMLITKCEAVASALDSEIKAEIKA--- 120

Query: 121 GTILKPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRRVQP 180
                              +GI+LKPK E VAKSA+QI++EQR N APAARM+MTRRV P
Sbjct: 121 ------------------STGIMLKPKHEFVAKSASQIINEQRGNAAPAARMAMTRRVHP 180

Query: 181 LVYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKF 240
           LV LNPY G WTIKVRVT+KG +RTYKNA+GEGCVFNV LTDE+GTQIQATMFN AARKF
Sbjct: 181 LVSLNPYQGSWTIKVRVTNKGVMRTYKNARGEGCVFNVELTDEEGTQIQATMFNAAARKF 240

Query: 241 YEKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVK 300
           Y++F +GKVYY+SRG+LK+ANK+FKTVQNDYEMTLN NS+V+E SNE  F PETK+NFV 
Sbjct: 241 YDRFEMGKVYYISRGSLKLANKQFKTVQNDYEMTLNENSEVEEASNEEMFTPETKFNFVP 300

Query: 301 IDMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWND 360
           ID LG +VN +DL+DVIGVVQ+VS TMSIRRK +NE  PKRD+T+ADETKKTVV+SLWND
Sbjct: 301 IDELGTYVNQKDLIDVIGVVQSVSPTMSIRRKNDNEMIPKRDITLADETKKTVVVSLWND 360

Query: 361 LATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDS 420
           LAT +GQELLD+AD  P+IA+K L+VG FQGVSLSTISRS++ INP++ EA  L+SWYD+
Sbjct: 361 LATGIGQELLDMADNHPVIAIKSLKVGAFQGVSLSTISRSNVVINPNSPEATKLKSWYDA 420

Query: 421 EGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKP 480
           EGK  SMS++ SG+S S  +GSRSMYSDR+ LSHI  N SLGEEKP FFS RAY+SFIKP
Sbjct: 421 EGKETSMSAIGSGMSSSANNGSRSMYSDRVFLSHITSNPSLGEEKPVFFSTRAYISFIKP 480

Query: 481 DQTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVSTFNE 540
           DQTMWYRACKTCNKKVTEA+ SGYWC++CQK D+ECSLRYIM V+VSD++GE W+S FN+
Sbjct: 481 DQTMWYRACKTCNKKVTEAMDSGYWCESCQKKDQECSLRYIMAVKVSDSTGETWLSAFND 540

Query: 541 EAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRITVR 600
           EAE+I+GC+AD+L+ LKS+ GE N +Q KLKEATW  HLFR+SVSQ EYN+EKRQRITVR
Sbjct: 541 EAEKIIGCTADDLNDLKSEEGEVNEFQTKLKEATWSSHLFRISVSQQEYNSEKRQRITVR 600

Query: 601 SVAPVDFAAESRFLLEEIAKMKAS 624
            V+P+DFAAE+R LL++I+K K S
Sbjct: 601 GVSPIDFAAETRLLLQDISKNKTS 603

BLAST of Chy2G047070 vs. TAIR 10
Match: AT5G61000.1 (Replication factor-A protein 1-related )

HSP 1 Score: 842.4 bits (2175), Expect = 2.3e-244
Identity = 417/622 (67.04%), Postives = 521/622 (83.76%), Query Frame = 0

Query: 1   MASAPTAGGISKVLSNPSPDSPSDVPDVVVQVIDLKATGNRYMFTASDGDMKLKAILPSN 60
           M ++ T   IS VLSNPS DS SD  ++VVQV+DLK  GNRY F+A+DG  K+KA+  ++
Sbjct: 1   MQTSVTPDAISTVLSNPSFDSSSDRSEIVVQVVDLKPIGNRYTFSANDGKTKVKAMFTAS 60

Query: 61  LTSDVISGNIQNLGLIRILDYSLNDIPNKSEKYLIVTKCEVVSPALEKEVKTEVKT--EE 120
           LT ++ISG IQNLGLIR++D+++NDI +KS KY +VTKCE V   L+ E+  + K+  EE
Sbjct: 61  LTPEIISGKIQNLGLIRLIDFTVNDISSKSTKYFLVTKCEAVGSVLDSEINLDSKSGEEE 120

Query: 121 AGTILKPKVELDDKSTFNN--GSGIILKPKQELVAKSAAQIVHEQRMNMAPAARMSMTRR 180
           A    K K+E    S  N+   +GI LKPKQE VAKSA+QI+ EQR N APAARM+MTRR
Sbjct: 121 AREPKKQKLEHSPVSPLNDVVSTGITLKPKQEFVAKSASQIMSEQRGNAAPAARMAMTRR 180

Query: 181 VQPLVYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAA 240
           V PLV LNPY G+WTIKVRVT+KG +R YKNA+GEGCVFNV LTDE+GTQIQATMFNDAA
Sbjct: 181 VHPLVSLNPYQGNWTIKVRVTNKGVMRNYKNARGEGCVFNVELTDEEGTQIQATMFNDAA 240

Query: 241 RKFYEKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYN 300
           RKF+++F LGKVYY+SRG+LK+ANK+FKTVQNDYEMTLN NS+V+E S+E  F+PETK+N
Sbjct: 241 RKFFDRFQLGKVYYISRGSLKLANKQFKTVQNDYEMTLNENSEVEEASSEEMFIPETKFN 300

Query: 301 FVKIDMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISL 360
           FV I+ LG +VN ++L+D+IGVVQ+VS TMSIRR+ +NE  PKRD+T+ADE++KTVV+SL
Sbjct: 301 FVPIEELGLYVNQKELIDLIGVVQSVSPTMSIRRRTDNEMIPKRDITLADESRKTVVVSL 360

Query: 361 WNDLATTVGQELLDIADQSPIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSW 420
           WNDLAT +GQELLD+ADQSP+IA+K L+VGDFQGVSLSTISRS++ INP++ EA  L+SW
Sbjct: 361 WNDLATGIGQELLDMADQSPVIAIKSLKVGDFQGVSLSTISRSNVVINPESPEAKKLKSW 420

Query: 421 YDSEGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSF 480
           +DSEGK +SMSS+ SG+SPS K+GSRS+Y+DR+ LSHI  N SL EEKP FFS RAY+SF
Sbjct: 421 FDSEGKEISMSSIGSGMSPSAKNGSRSLYTDRVLLSHITSNPSLFEEKPVFFSTRAYISF 480

Query: 481 IKPDQTMWYRACKTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVST 540
           IKPDQTMWY+ACKTCNKKVTEA+ SGYWC+ CQ+  EECSLRYIM V+V+D+SGE W+S+
Sbjct: 481 IKPDQTMWYQACKTCNKKVTEALDSGYWCEGCQRKYEECSLRYIMAVKVTDSSGETWISS 540

Query: 541 FNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQRI 600
           FN+EAE+I+GCSADEL++LKS+ GE N YQ KLKEATW  H+FRVSV+QNEYN EKRQR+
Sbjct: 541 FNDEAEKILGCSADELNKLKSEEGEVNEYQTKLKEATWSSHVFRVSVTQNEYNGEKRQRV 600

Query: 601 TVRSVAPVDFAAESRFLLEEIA 619
           TV+ VAP+DFAAE+R LL++I+
Sbjct: 601 TVKGVAPLDFAAETRLLLQDIS 622

BLAST of Chy2G047070 vs. TAIR 10
Match: AT2G06510.1 (replication protein A 1A )

HSP 1 Score: 307.4 bits (786), Expect = 2.7e-83
Identity = 194/627 (30.94%), Postives = 331/627 (52.79%), Query Frame = 0

Query: 29  VVQVIDLKATG-------NRYMFTASDGDMKLKAILPSNLTSDVISGNIQNLGLIRILDY 88
           ++QV+++K  G        RY F  SDG     A++   L   V SG  +   +++++DY
Sbjct: 23  LLQVLEIKMIGRSQERSQERYRFLISDGVSAQHAMVAVQLNDRVKSGQFEKGSIVQLIDY 82

Query: 89  SLNDIPNKSEKYLIVTKCEVV--------SPALEKEVKTEVKTEEAGT----------IL 148
             +D+  K  K ++V   E +        +P +  E  TE +   +GT            
Sbjct: 83  ICSDV--KGRKLIVVLNMETIVQQSETIGNPTIFGETDTEAQKTFSGTGNIPPPNRVVFN 142

Query: 149 KPKVELDDKSTFNNGSGIILKPKQELVAKSAAQIVHE---QRMNMAPAARMSMTRRVQPL 208
           +P V+         G  I  +       + + Q  ++      N  P  +     RV P+
Sbjct: 143 EPMVQHSVNRAPPRGVNIQNQANNTPSFRPSVQPSYQPPASYRNHGPIMKNEAPARVIPI 202

Query: 209 VYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFY 268
             LNPY G W IK RVT KG++R Y NA+G+G VF+  L D DG +I+ T FN    +FY
Sbjct: 203 AALNPYQGRWAIKARVTAKGDIRRYNNAKGDGKVFSFDLLDYDGGEIRVTCFNALVDRFY 262

Query: 269 EKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKI 328
           +   +GKVY +S+G+LK A K F  ++N++E+ L   S V+   +E   +P+ +++F  I
Sbjct: 263 DVTEVGKVYLISKGSLKPAQKNFNHLKNEWEIFLESTSTVELCPDEDGSIPKQQFSFRPI 322

Query: 329 DMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDL 388
             +  +     ++DVIGVV +V+ ++ I RK   ET  +R + + DE+ K V ++LW + 
Sbjct: 323 SDI-ENAENNTILDVIGVVTSVNPSVPILRKNGMET-HRRILNLKDESGKAVEVTLWGEF 382

Query: 389 ATTVGQELLDIADQS--PIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYD 448
               G++L ++ D +  P++A+K  +V DF G S+ TIS + + INPD  EAH LR+W+D
Sbjct: 383 CNRDGRQLEEMVDSAFHPVLAIKAGKVSDFSGKSVGTISSTQLFINPDFPEAHKLRTWFD 442

Query: 449 SEGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIK 508
             GK     + S  +S  T  G  S    R ++S I E      +KP + +++A +SFIK
Sbjct: 443 YGGK----DTASFSISRDTMPGGVSRNEIRKNVSQIKEEGLGRSDKPDWITVKATISFIK 502

Query: 509 PDQTMWYRAC------KTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEA 568
            D +  Y AC      K CNKKVT +  + + CD C +  +EC  RY++ V++ D +G  
Sbjct: 503 TD-SFCYTACPLMIGDKQCNKKVTRSGTNRWLCDRCNQESDECDYRYLLQVQIQDHTGLT 562

Query: 569 WVSTFNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEK 620
           W++ F E  E IMGC A +L  +K ++ +E  +   +++  +  ++ ++ + +  Y +E+
Sbjct: 563 WITAFQETGEEIMGCPAKKLYAMKYELEKEEEFAEIVRDRLFHQYMLKLKIKEESYGDEQ 622

BLAST of Chy2G047070 vs. TAIR 10
Match: AT2G06510.2 (replication protein A 1A )

HSP 1 Score: 304.7 bits (779), Expect = 1.7e-82
Identity = 193/624 (30.93%), Postives = 328/624 (52.56%), Query Frame = 0

Query: 32  VIDLKATG-------NRYMFTASDGDMKLKAILPSNLTSDVISGNIQNLGLIRILDYSLN 91
           V+++K  G        RY F  SDG     A++   L   V SG  +   +++++DY  +
Sbjct: 3   VLEIKMIGRSQERSQERYRFLISDGVSAQHAMVAVQLNDRVKSGQFEKGSIVQLIDYICS 62

Query: 92  DIPNKSEKYLIVTKCEVV--------SPALEKEVKTEVKTEEAGT----------ILKPK 151
           D+  K  K ++V   E +        +P +  E  TE +   +GT            +P 
Sbjct: 63  DV--KGRKLIVVLNMETIVQQSETIGNPTIFGETDTEAQKTFSGTGNIPPPNRVVFNEPM 122

Query: 152 VELDDKSTFNNGSGIILKPKQELVAKSAAQIVHE---QRMNMAPAARMSMTRRVQPLVYL 211
           V+         G  I  +       + + Q  ++      N  P  +     RV P+  L
Sbjct: 123 VQHSVNRAPPRGVNIQNQANNTPSFRPSVQPSYQPPASYRNHGPIMKNEAPARVIPIAAL 182

Query: 212 NPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDEDGTQIQATMFNDAARKFYEKF 271
           NPY G W IK RVT KG++R Y NA+G+G VF+  L D DG +I+ T FN    +FY+  
Sbjct: 183 NPYQGRWAIKARVTAKGDIRRYNNAKGDGKVFSFDLLDYDGGEIRVTCFNALVDRFYDVT 242

Query: 272 ALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDEVSNEAAFVPETKYNFVKIDML 331
            +GKVY +S+G+LK A K F  ++N++E+ L   S V+   +E   +P+ +++F  I  +
Sbjct: 243 EVGKVYLISKGSLKPAQKNFNHLKNEWEIFLESTSTVELCPDEDGSIPKQQFSFRPISDI 302

Query: 332 GPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDVTIADETKKTVVISLWNDLATT 391
             +     ++DVIGVV +V+ ++ I RK   ET  +R + + DE+ K V ++LW +    
Sbjct: 303 -ENAENNTILDVIGVVTSVNPSVPILRKNGMET-HRRILNLKDESGKAVEVTLWGEFCNR 362

Query: 392 VGQELLDIADQS--PIIALKYLRVGDFQGVSLSTISRSSITINPDTKEAHDLRSWYDSEG 451
            G++L ++ D +  P++A+K  +V DF G S+ TIS + + INPD  EAH LR+W+D  G
Sbjct: 363 DGRQLEEMVDSAFHPVLAIKAGKVSDFSGKSVGTISSTQLFINPDFPEAHKLRTWFDYGG 422

Query: 452 KAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSLGEEKPTFFSIRAYVSFIKPDQ 511
           K     + S  +S  T  G  S    R ++S I E      +KP + +++A +SFIK D 
Sbjct: 423 K----DTASFSISRDTMPGGVSRNEIRKNVSQIKEEGLGRSDKPDWITVKATISFIKTD- 482

Query: 512 TMWYRAC------KTCNKKVTEAIGSGYWCDNCQKNDEECSLRYIMVVRVSDASGEAWVS 571
           +  Y AC      K CNKKVT +  + + CD C +  +EC  RY++ V++ D +G  W++
Sbjct: 483 SFCYTACPLMIGDKQCNKKVTRSGTNRWLCDRCNQESDECDYRYLLQVQIQDHTGLTWIT 542

Query: 572 TFNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEATWVPHLFRVSVSQNEYNNEKRQR 620
            F E  E IMGC A +L  +K ++ +E  +   +++  +  ++ ++ + +  Y +E+R +
Sbjct: 543 AFQETGEEIMGCPAKKLYAMKYELEKEEEFAEIVRDRLFHQYMLKLKIKEESYGDEQRVK 602

BLAST of Chy2G047070 vs. TAIR 10
Match: AT5G45400.1 (Replication factor-A protein 1-related )

HSP 1 Score: 285.4 bits (729), Expect = 1.1e-76
Identity = 160/468 (34.19%), Postives = 262/468 (55.98%), Query Frame = 0

Query: 163 MNMAPAARMSMTRRVQPLVYLNPYLGDWTIKVRVTDKGNLRTYKNAQGEGCVFNVVLTDE 222
           +N  P AR     R+ P+  LNPY G WTIKVRVT K +LR + N +GEG +F+  L D 
Sbjct: 285 VNRGPVARNEAPPRINPIAALNPYQGRWTIKVRVTSKADLRRFNNPRGEGKLFSFDLLDA 344

Query: 223 DGTQIQATMFNDAARKFYEKFALGKVYYVSRGTLKVANKKFKTVQNDYEMTLNVNSQVDE 282
           DG +I+ T FNDA  +F++K  +G VY +SRG LK A K F  + NDYE+ L+  S +  
Sbjct: 345 DGGEIRVTCFNDAVDQFFDKIVVGNVYLISRGNLKPAQKNFNHLPNDYEIHLDSASTIQP 404

Query: 283 VSNEAAFVPETKYNFVKIDMLGPHVNGRDLVDVIGVVQNVSSTMSIRRKLNNETAPKRDV 342
             ++   +P   ++F  I  +  ++      DVIG+V ++S T++I RK N     KR +
Sbjct: 405 CEDDGT-IPRYHFHFRNIGDI-ENMENNSTTDVIGIVSSISPTVAIMRK-NLTEVQKRSL 464

Query: 343 TIADETKKTVVISLWNDLATTVGQELLDIADQS--PIIALKYLRVGDFQGVSLSTISRSS 402
            + D + ++V +++W +     GQ+L ++ D    P++ALK  R+G+F G  +STI  S 
Sbjct: 465 QLKDMSGRSVEVTMWGNFCNAEGQKLQNLCDSGVFPVLALKAGRIGEFNGKQVSTIGASQ 524

Query: 403 ITINPDTKEAHDLRSWYDSEGKAVSMSSVSSGLSPSTKSGSRSMYSDRISLSHILENKSL 462
             I PD  EA +LR WY+ EG+    +S+S   S   +   R +      ++ I + K  
Sbjct: 525 FFIEPDFPEARELRQWYEREGRNAHFTSISREFSGVGRQEVRKV------IAQIKDEKLG 584

Query: 463 GEEKPTFFSIRAYVSFIKPDQTMWYRAC------KTCNKKVTEAIGSGYW-CDNCQKNDE 522
             EKP + ++ A +SF+K  +   Y AC      + C+KKVT   G G W C+ C K  +
Sbjct: 585 TSEKPDWITVCATISFMKV-ENFCYTACPIMNGDRPCSKKVTNN-GDGTWRCEKCDKCVD 644

Query: 523 ECSLRYIMVVRVSDASGEAWVSTFNEEAERIMGCSADELDQLKSQIGEENSYQLKLKEAT 582
           EC  RYI+ +++ D +   W + F E  E IMG SA +L  +K +  +E  ++  ++   
Sbjct: 645 ECDYRYILQIQLQDHTDLTWATAFQEAGEEIMGMSAKDLYYVKYENQDEEKFEDIIRSVA 704

Query: 583 WVPHLFRVSVSQNEYNNEKRQRITVRSVAPVDFAAESRFLLEEIAKMK 622
           +  ++F++ + +  Y++E+R + TV     +++++ +RF+LE I K+K
Sbjct: 705 FTKYIFKLKIKEETYSDEQRVKATVVKAEKLNYSSNTRFMLEAIDKLK 741

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD823.1e-24667.79Replication protein A 70 kDa DNA-binding subunit B OS=Arabidopsis thaliana OX=37... [more]
Q9FME03.2e-24367.04Replication protein A 70 kDa DNA-binding subunit D OS=Arabidopsis thaliana OX=37... [more]
Q10Q084.1e-23064.70Replication protein A 70 kDa DNA-binding subunit B OS=Oryza sativa subsp. japoni... [more]
Q9SKI43.8e-8230.94Replication protein A 70 kDa DNA-binding subunit A OS=Arabidopsis thaliana OX=37... [more]
Q6YZ497.8e-8029.92Replication protein A 70 kDa DNA-binding subunit A OS=Oryza sativa subsp. japoni... [more]
Match NameE-valueIdentityDescription
A0A0A0LSG20.0e+0099.36Replication protein A subunit OS=Cucumis sativus OX=3659 GN=Csa_1G013800 PE=3 SV... [more]
A0A5A7UTE70.0e+0096.31Replication protein A subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BX020.0e+0096.31Replication protein A subunit OS=Cucumis melo OX=3656 GN=LOC103494045 PE=3 SV=1[more]
A0A6J1F0850.0e+0090.21Replication protein A subunit OS=Cucurbita moschata OX=3662 GN=LOC111440985 PE=3... [more]
A0A6J1I6K60.0e+0090.05Replication protein A subunit OS=Cucurbita maxima OX=3661 GN=LOC111470404 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_011648462.10.099.36replication protein A 70 kDa DNA-binding subunit B [Cucumis sativus] >KGN63732.1... [more]
XP_008453278.10.096.31PREDICTED: replication protein A 70 kDa DNA-binding subunit B [Cucumis melo] >KA... [more]
XP_038878718.10.094.22replication protein A 70 kDa DNA-binding subunit B-like [Benincasa hispida][more]
KAG6587586.10.090.53Replication protein A 70 kDa DNA-binding subunit B, partial [Cucurbita argyrospe... [more]
XP_022933609.10.090.21replication protein A 70 kDa DNA-binding subunit B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G08020.12.2e-24767.79RPA70-kDa subunit B [more]
AT5G61000.12.3e-24467.04Replication factor-A protein 1-related [more]
AT2G06510.12.7e-8330.94replication protein A 1A [more]
AT2G06510.21.7e-8230.93replication protein A 1A [more]
AT5G45400.11.1e-7634.19Replication factor-A protein 1-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 538..558
NoneNo IPR availableGENE3D2.40.50.140coord: 176..285
e-value: 8.8E-34
score: 118.0
NoneNo IPR availableGENE3D2.40.50.140coord: 290..431
e-value: 4.5E-38
score: 132.1
NoneNo IPR availableGENE3D2.40.50.140coord: 432..620
e-value: 1.0E-52
score: 180.2
NoneNo IPR availableGENE3D2.40.50.140coord: 6..113
e-value: 1.5E-13
score: 52.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR23273:SF32REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT B-RELATEDcoord: 7..618
NoneNo IPR availablePANTHERPTHR23273REPLICATION FACTOR A 1, RFA1coord: 7..618
NoneNo IPR availableCDDcd04475RPA1_DBD_Bcoord: 312..418
e-value: 1.34933E-34
score: 124.619
NoneNo IPR availableCDDcd04474RPA1_DBD_Acoord: 179..282
e-value: 5.13139E-43
score: 147.778
IPR013955Replication factor A, C-terminalPFAMPF08646Rep_fac-A_Ccoord: 467..613
e-value: 4.7E-46
score: 156.2
IPR013955Replication factor A, C-terminalCDDcd04476RPA1_DBD_Ccoord: 454..619
e-value: 4.20321E-54
score: 180.197
IPR031657Replication protein A, OB domainPFAMPF16900REPA_OB_2coord: 308..404
e-value: 1.9E-21
score: 75.8
IPR004365OB-fold nucleic acid binding domain, AA-tRNA synthetase-typePFAMPF01336tRNA_anti-codoncoord: 190..274
e-value: 4.9E-10
score: 39.3
IPR007199Replication factor-A protein 1, N-terminalPFAMPF04057Rep-A_Ncoord: 6..103
e-value: 1.4E-20
score: 73.1
IPR004591Replication factor A protein 1TIGRFAMTIGR00617TIGR00617coord: 6..619
e-value: 1.3E-188
score: 626.3
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 4..107
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 181..286
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 295..424
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 446..620

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G047070.1Chy2G047070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006268 DNA unwinding involved in DNA replication
biological_process GO:0000724 double-strand break repair via homologous recombination
biological_process GO:0051321 meiotic cell cycle
biological_process GO:0006289 nucleotide-excision repair
biological_process GO:0007004 telomere maintenance via telomerase
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
biological_process GO:0006260 DNA replication
cellular_component GO:0005662 DNA replication factor A complex
cellular_component GO:0005634 nucleus
molecular_function GO:0003684 damaged DNA binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0043047 single-stranded telomeric DNA binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003676 nucleic acid binding