Chy2G046940 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G046940
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	protein MIS12 homolog
Location	chrH02: 27953341 .. 27955884 (-)
RNA-Seq Expression	Chy2G046940
Synteny	Chy2G046940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAAGTAAAGGCGAAGCGGTGTTCAATTCACTCAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATACTGTCGACGATTTGGTTGATGATGCTTTCGATTTCTATCAATCGTATTGTTCTCTCTGTTTTCCATTTCTTTAATTTAGGTTTTTCAATTTTGTTCGCTTAATTTTGTTGATTCTGGTTAATTCTTTTTCCCTTCAGACAAGCATCGGCTGCCTTGAAGACCGAGGGCTCGGATAGATCTCAGGATCTTACCCTGGTAAGGTTTAATTTGTATTATGTTATTTGGCATTTTCTTGTTAATTTCTGAATTATTAAGTTTGTACTCTTAAGCACTTCTTGAATTGTTCCCATTTTCGCCTCGTTTTATTCAGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGTCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGTAGGTTCTTCTTCTACTCTGTCTTAATGAGGCTCCTTGGCTGGTATATCAACATTGACAGATATTTATGTAAAACAATAAATTAACAGAAATTATTTAAATTAGCTATTGAATTGCTTTTGCTTTCAAACTAAGTTGTAGATCTTGCTTTTAAGTAGTTTTCATATTGAGATTAATAGATGACATCTTTTTATGTTTTATGTGTTTATAAAGGATAGTTGAGATGTTTATAAACCCTTAATATTGATGTCAAATCTTCATATATATATATATATATATAACTTCGCGCGTTCAAAAGTTCTCTATAAATATAACTTTGCATGTAAATTGACTGGTTGCTTATGCAGCGAGTTTCGCATTTATTTTATCCTGACTTGATAACTTCCCCAATTTTTGGTTCTGGGACGAACAAATTTTGAGGAGAAAGCCCAGTTACTTACTGTGCATCATTGAGTTTCAATTTTTGTTCCAAATATATTCATTGCTACTTTCAGATATAATCACGAGTTTCCATATTTTCAGACCTCAGAGCTTCCTCATTTTGTTAGCAATAACTCAACGTGGTTCCATTGTAACTAGAATGTGGTCATGACGGTAAAAGATTCTTTTCAGCTCTAAAAGGAATGCGACCAAATGCCTGCAGCACTTTTAAAAATCTGTCACTGGGGCATCGTGCCTTTTCTTTTTCAATTACATTTGAAGTCTCCTATGTAAGATCCAGTTCGATTCCTTTCATTTTGCACCAATATAGTTTACTTTGGGTTGGGCAACTTCTGTCTGTTTCACTGGCATCTCATTGGAATCCCCAATATCTTATTGTCAGGATGCTAAATTATCAATGGTTCCCATCTTGTTTGAGAACTTTGAGTTATGGCTAATTGAGTCTATGTGCTCTTTTAATCGAGTCTGTGTTCTTTTACTACTTTTTCTTCTTTCTAATGCAATAGCTAATAAACATATTTCTGATTCAGATCTAAATATTTTCTTTTAAGTTGTCTAAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAACTTGATCTTTTGAGAAATAAGCTCTCCGAGGTATTGAATATGTCGTATGTTTAACACGTGCGTTTATGTAAATCATATTTCATGAGGAAATATATTCTTCAGGTCAGAAAAGAGAACATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAATTAAGTTGTGCTTAAAACCAATTTTTTCTTCCTGCACTCCTGGGTTTACGTGCACACTACTCTACTAGTACACTCATGAAGGATTCCTCCAAATGAAATATACAAATAATAGCCTCCTCCGGCGGATCATCCTGGTTTGCATGTCTCATGGTGACACACTCTAATAGTTCCAAATGACACTTAAAATCATTTCATTTAGGCAATTTTTATATTACCCTAAACTCTAATAGTCTCATTTTCTGTTCATTCCATAAAAGAGGAGCTTCTTGCAGTATGTGAATAAGGCAACTTCTCTTGCTATTTTCATTTTGAATGCCACTTGAACAAAATTTATTCTTTAGTTATTAAATTTCGTTGTACTGACACTTCAACAGTCGGCAGGTAAAAGTTCATGATTATAGGAAGCAGAATTCTAACATATATTGATTTCTTGCAGAAATGATCAGAACTGCATCGGAGCTTCGTGCGAAAATAGGAAAGCTGAAGAAAAGTAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGATTCATACAAATGGAGACATCTCTCACCATCACAAGGGTAGGATTTTCAAGCTACTTTATTATCAATGAAGCTGGTTTTAGCTTTTTTGAAGCTGAATATCATCTCACATGCTCGTTGATGCTTGTTTCTGTTGTGCAGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGA

mRNA sequence

ATGGAAGGAAGTAAAGGCGAAGCGGTGTTCAATTCACTCAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATACTGTCGACGATTTGGTTGATGATGCTTTCGATTTCTATCAATCACAAGCATCGGCTGCCTTGAAGACCGAGGGCTCGGATAGATCTCAGGATCTTACCCTGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGTCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATTTGTCTAAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAACTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAACATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAGAACTGCATCGGAGCTTCGTGCGAAAATAGGAAAGCTGAAGAAAAGTAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGATTCATACAAATGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGA

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAAGCGGTGTTCAATTCACTCAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATACTGTCGACGATTTGGTTGATGATGCTTTCGATTTCTATCAATCACAAGCATCGGCTGCCTTGAAGACCGAGGGCTCGGATAGATCTCAGGATCTTACCCTGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGTCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATTTGTCTAAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAACTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAACATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAGAACTGCATCGGAGCTTCGTGCGAAAATAGGAAAGCTGAAGAAAAGTAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGATTCATACAAATGGAGACATCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGA

Protein sequence

MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI*

Homology

[Close]

BLAST of Chy2G046940 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 3.3e-50
Identity = 116/250 (46.40%), Postives = 174/250 (69.60%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK EAVF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHD--H 120
           + GI++VR L+   +  RL +WE Y L  CF+VP+GF LP +   +ES   +S+  D  +
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKS---EES---SSVHQDGLY 120

Query: 121 DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVND 180
           D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS+++
Sbjct: 121 DLELDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDE 180

Query: 181 MFQEMIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFS----NAKLDDI 240
           +F+EM + ASELRA + +LK  R + ++ A V++      + +H K FS    + KL+D+
Sbjct: 181 IFKEMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDL 238

Query: 241 QEFLSDLKKI 243
           ++F ++L+K+
Sbjct: 241 EKFQAELRKM 238

[Close]

BLAST of Chy2G046940 vs. ExPASy TrEMBL
Match: A0A0A0LPL2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.1e-120
Identity = 232/242 (95.87%), Postives = 234/242 (96.69%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDL 120
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+   DESPGVTSISHDHDVDL
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD---DESPGVTSISHDHDVDL 120

Query: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180
           DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE
Sbjct: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180

Query: 181 MIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLK 240
           MIRTASELR KIGKLKK RTEETKLAIVEK+HTNGDISHHHKGFSNAKLDDIQEFLS LK
Sbjct: 181 MIRTASELRVKIGKLKK-RTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSGLK 238

Query: 241 KI 243
           KI
Sbjct: 241 KI 238

[Close]

BLAST of Chy2G046940 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 2.7e-119
Identity = 228/242 (94.21%), Postives = 233/242 (96.28%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDL 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN   DESPGVTSI+HD DVDL
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DESPGVTSINHDQDVDL 120

Query: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180
           DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE
Sbjct: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180

Query: 181 MIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLK 240
           MI+TASELRAKIGK+KK RTEE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLK
Sbjct: 181 MIKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLK 239

Query: 241 KI 243
           KI
Sbjct: 241 KI 239

[Close]

BLAST of Chy2G046940 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.7e-118
Identity = 226/242 (93.39%), Postives = 232/242 (95.87%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDL 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN   DESPGVTSI+HD DVDL
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DESPGVTSINHDQDVDL 120

Query: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180
           DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE
Sbjct: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180

Query: 181 MIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLK 240
           MI+TASELR+KIGK+KK RTEE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLK
Sbjct: 181 MIKTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLK 239

Query: 241 KI 243
           KI
Sbjct: 241 KI 239

[Close]

BLAST of Chy2G046940 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 8.3e-105
Identity = 205/244 (84.02%), Postives = 220/244 (90.16%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHD--HDV 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN   DESP VTS+S D  +DV
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DESPVVTSVSRDCLYDV 120

Query: 121 DLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMF 180
           DLDTELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN+MF
Sbjct: 121 DLDTELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMF 180

Query: 181 QEMIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSD 240
           QEM+ TASELRAKIGKLKK R EE+KL  VEKIHTNGD SHHHKGFSNAKLDDIQEFL+D
Sbjct: 181 QEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLAD 240

Query: 241 LKKI 243
           LK +
Sbjct: 241 LKAL 241

[Close]

BLAST of Chy2G046940 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.1e-104
Identity = 205/244 (84.02%), Postives = 219/244 (89.75%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHD--HDV 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN   DESP VTS+S D  +DV
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DESPVVTSVSRDCLYDV 120

Query: 121 DLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMF 180
           DLDTELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MF
Sbjct: 121 DLDTELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMF 180

Query: 181 QEMIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSD 240
           QEM+ TASELRAKIGKLKK R EE+KL  VEKIHTN DISHHHKGFSNAKLDDIQEFL+D
Sbjct: 181 QEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLAD 240

Query: 241 LKKI 243
           LK +
Sbjct: 241 LKTL 241

[Close]

BLAST of Chy2G046940 vs. NCBI nr
Match: XP_004138204.3 (protein MIS12 homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 441 bits (1134), Expect = 2.01e-155
Identity = 233/242 (96.28%), Postives = 235/242 (97.11%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDL 120
           GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+   DESPGVTSISHDHDVDL
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD---DESPGVTSISHDHDVDL 120

Query: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180
           DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE
Sbjct: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180

Query: 181 MIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLK 240
           MIRTASELR KIGKLKK RTEETKLAIVEK+HTNGDISHHHKGFSNAKLDDIQEFLSDLK
Sbjct: 181 MIRTASELRVKIGKLKK-RTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLK 238

Query: 241 KI 242
           KI
Sbjct: 241 KI 238

[Close]

BLAST of Chy2G046940 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 435 bits (1119), Expect = 4.02e-153
Identity = 228/242 (94.21%), Postives = 233/242 (96.28%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDL 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN   DESPGVTSI+HD DVDL
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DESPGVTSINHDQDVDL 120

Query: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180
           DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE
Sbjct: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180

Query: 181 MIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLK 240
           MI+TASELRAKIGK+KK RTEE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLK
Sbjct: 181 MIKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLK 239

Query: 241 KI 242
           KI
Sbjct: 241 KI 239

[Close]

BLAST of Chy2G046940 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 432 bits (1112), Expect = 4.69e-152
Identity = 226/242 (93.39%), Postives = 232/242 (95.87%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDL 120
           GISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN   DESPGVTSI+HD DVDL
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DESPGVTSINHDQDVDL 120

Query: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180
           DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE
Sbjct: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180

Query: 181 MIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLK 240
           MI+TASELR+KIGK+KK RTEE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLK
Sbjct: 181 MIKTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLK 239

Query: 241 KI 242
           KI
Sbjct: 241 KI 239

[Close]

BLAST of Chy2G046940 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 399 bits (1024), Expect = 3.50e-138
Identity = 209/242 (86.36%), Postives = 223/242 (92.15%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGEAVF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTEGSDRSQDLTL
Sbjct: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHDHDVDL 120
           GIS+VRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN   D+SPGVTSISHD+DV L
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DDSPGVTSISHDYDVGL 150

Query: 121 DTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQE 180
           DTELD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQE
Sbjct: 151 DTELDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQE 210

Query: 181 MIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLK 240
           M+ TASELRAKIGKLKK R EE+KL  VEK+HTNGDISH+H G SNAKLDDI+ FL DLK
Sbjct: 211 MMGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLK 269

Query: 241 KI 242
            +
Sbjct: 271 TL 269

[Close]

BLAST of Chy2G046940 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 393 bits (1009), Expect = 2.49e-136
Identity = 207/244 (84.84%), Postives = 223/244 (91.39%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL+L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHD--HDV 120
           GIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN   DESP VTS+S D  +DV
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSN---DESPVVTSVSRDCLYDV 120

Query: 121 DLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMF 180
           DLDTELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MF
Sbjct: 121 DLDTELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMF 180

Query: 181 QEMIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSD 240
           QEM+ TASELRAKIGKLKK R EE+KL  VEKIHTNGDISHHHKGFSNAKLDDIQEFL+D
Sbjct: 181 QEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLAD 240

Query: 241 LKKI 242
           LK +
Sbjct: 241 LKTL 241

[Close]

BLAST of Chy2G046940 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 199.9 bits (507), Expect = 2.3e-51
Identity = 116/250 (46.40%), Postives = 174/250 (69.60%), Query Frame = 0


Query: 1   MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK EAVF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVTSISHD--H 120
           + GI++VR L+   +  RL +WE Y L  CF+VP+GF LP +   +ES   +S+  D  +
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKS---EES---SSVHQDGLY 120

Query: 121 DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVND 180
           D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS+++
Sbjct: 121 DLELDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDE 180

Query: 181 MFQEMIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHHHKGFS----NAKLDDI 240
           +F+EM + ASELRA + +LK  R + ++ A V++      + +H K FS    + KL+D+
Sbjct: 181 IFKEMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDL 238

Query: 241 QEFLSDLKKI 243
           ++F ++L+K+
Sbjct: 241 EKFQAELRKM 238

The following BLAST results are available for this feature:

BLAST of Chy2G046940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	3.3e-50	46.40	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of Chy2G046940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPL2	3.1e-120	95.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1	[more]
A0A5A7US57	2.7e-119	94.21	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7UQ95	1.7e-118	93.39	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A6J1E0A1	8.3e-105	84.02	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JME3	3.1e-104	84.02	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of Chy2G046940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004138204.3	2.01e-155	96.28	protein MIS12 homolog isoform X1 [Cucumis sativus]	[more]
KAA0058004.1	4.02e-153	94.21	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]
KAA0058032.1	4.69e-152	93.39	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]
XP_038879945.1	3.50e-138	86.36	protein MIS12 homolog [Benincasa hispida]	[more]
XP_023515499.1	2.49e-136	84.84	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy2G046940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	2.3e-51	46.40	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 124..151
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 11..142 e-value: 8.6E-19 score: 68.0
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G046940.1	Chy2G046940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus