Chy2G046400 (gene) Cucumber (hystrix) v1

Overview


Name	Chy2G046400
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	protein ASPARTIC PROTEASE IN GUARD CELL 1
Location	chrH02: 27562511 .. 27563983 (-)
RNA-Seq Expression	Chy2G046400
Synteny	Chy2G046400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAACACTTCACTTTCCTATGCTTTTCTCTTCCTAACATTCTTCACTTTCCTTCACTTCCCATCAATTCTCTCTCGCAAACTAACAACTCAATCTCCTTATTCCACTAATACCTTTGATGTCTCGGCCTCCATTAACCAAGCCTTAAATGCCCTTTCCATCAAACCCAAACCCTTTCAAACGACTCACTCTAATTACCATTCAAGTTCACCTCTATCTCTCTCATTGCACCCTAGATTGACCGTTCATAACCCTTCCTACGAGGACTATGGTAGTCTTGTTAGAGCCCGACTTGCTCGTGGTGCCGCCCGAGTTCAATCCCTTAATAGAAAGCTTGAGCTCTCTTTAAAAGGGGGTAAACAATTTGGTAGAAGAATTAATGGATCTGATTCTACAAATTCACTTACTGTTCCCGTTACTTCAGGGGCGAGTCAAGGGGCTGGCGAATATTTTGCCCGGATTGGAGTCGGTCAACCTGTGCAATCGTATTTTTTTGTGCCTGATACTGGAAGTGATGTTTCGTGGCTACAATGTCAGCCATGTGATGGTGAGAATGGTTGTTATAAACAAATTGGCCCGATATTTGACCCGAAATCGTCGTCTTCTTATAGTCCACTGTCTTGTGATTCGGAACAATGTCATTTGTTGGATGAAGCTGCTTGTGATGTCAATTCGTGTATCTACGAAGTTGAATATGGTGATGGATCATTCACAGTTGGTGAACTTGCCACCGAAACGTTCTCATTTCGACATTCTAATTCCATCCCTAATCTCCCGATTGGTTGTGGTCATGACAATGAAGGCCTCTTTATTGGGGCTGCTGGTTTAATTGGCCTCGGTGGTGGGGCAATTTCCCTTTCTTCCCAATTAGAAGCGTCGTCATTTTCATATTGTCTGGTCGACCTAGACTCAGAATCATCCTCCACTCTTGACTTCAACGCAGACCAACCAAGCGACTCACTCACCTCCCCACTTGTGAAAAATGATCGATTCCCCACGTTTCGGTACGTGAAAGTAATTGGGATGAGCGTCGGCGGAAAACCTTTACCGATTTCCTCGTCAAGCTTTGAAATCGACGAATCAGGATCAGGGGGAATAATCGTGGATTCTGGGACGACTATAACTGAACTACCAAGTGATGTGTATGGCGTGTTAAGAGACGCGTTCGTGGGGCTAACAAAGAACCTCCCACCAGCACCAGGGGTATCACCGTTTGATACTTGTTATGACTTATCAAGTCAATCGAATGTGGAGGTACCAACCATAGCATTTATTTTGTCGGGGGAAAACTCGCTACAACTACCAGCAAAAAATTGTTTGATCCAAGTGGACTCAGCTGGAACTTTCTGCTTGGCGTTTCTTCCATCAACATTTCCACTTTCTATTATTGGGAATGTCCAACAGCAAGGAATACGTGTCAGCTATGACTTGGCCAACTCTCTCGTCGGATTCTCGACTGATAAATGTTAA

mRNA sequence

ATGATGAACACTTCACTTTCCTATGCTTTTCTCTTCCTAACATTCTTCACTTTCCTTCACTTCCCATCAATTCTCTCTCGCAAACTAACAACTCAATCTCCTTATTCCACTAATACCTTTGATGTCTCGGCCTCCATTAACCAAGCCTTAAATGCCCTTTCCATCAAACCCAAACCCTTTCAAACGACTCACTCTAATTACCATTCAAGTTCACCTCTATCTCTCTCATTGCACCCTAGATTGACCGTTCATAACCCTTCCTACGAGGACTATGGTAGTCTTGTTAGAGCCCGACTTGCTCGTGGTGCCGCCCGAGTTCAATCCCTTAATAGAAAGCTTGAGCTCTCTTTAAAAGGGGGTAAACAATTTGGTAGAAGAATTAATGGATCTGATTCTACAAATTCACTTACTGTTCCCGTTACTTCAGGGGCGAGTCAAGGGGCTGGCGAATATTTTGCCCGGATTGGAGTCGGTCAACCTGTGCAATCGTATTTTTTTGTGCCTGATACTGGAAGTGATGTTTCGTGGCTACAATGTCAGCCATGTGATGGTGAGAATGGTTGTTATAAACAAATTGGCCCGATATTTGACCCGAAATCGTCGTCTTCTTATAGTCCACTGTCTTGTGATTCGGAACAATGTCATTTGTTGGATGAAGCTGCTTGTGATGTCAATTCGTGTATCTACGAAGTTGAATATGGTGATGGATCATTCACAGTTGGTGAACTTGCCACCGAAACGTTCTCATTTCGACATTCTAATTCCATCCCTAATCTCCCGATTGGTTGTGGTCATGACAATGAAGGCCTCTTTATTGGGGCTGCTGGTTTAATTGGCCTCGGTGGTGGGGCAATTTCCCTTTCTTCCCAATTAGAAGCGTCGTCATTTTCATATTGTCTGGTCGACCTAGACTCAGAATCATCCTCCACTCTTGACTTCAACGCAGACCAACCAAGCGACTCACTCACCTCCCCACTTGTGAAAAATGATCGATTCCCCACGTTTCGGTACGTGAAAGTAATTGGGATGAGCGTCGGCGGAAAACCTTTACCGATTTCCTCGTCAAGCTTTGAAATCGACGAATCAGGATCAGGGGGAATAATCGTGGATTCTGGGACGACTATAACTGAACTACCAAGTGATGTGTATGGCGTGTTAAGAGACGCGTTCGTGGGGCTAACAAAGAACCTCCCACCAGCACCAGGGGTATCACCGTTTGATACTTGTTATGACTTATCAAGTCAATCGAATGTGGAGGTACCAACCATAGCATTTATTTTGTCGGGGGAAAACTCGCTACAACTACCAGCAAAAAATTGTTTGATCCAAGTGGACTCAGCTGGAACTTTCTGCTTGGCGTTTCTTCCATCAACATTTCCACTTTCTATTATTGGGAATGTCCAACAGCAAGGAATACGTGTCAGCTATGACTTGGCCAACTCTCTCGTCGGATTCTCGACTGATAAATGTTAA

Coding sequence (CDS)

ATGATGAACACTTCACTTTCCTATGCTTTTCTCTTCCTAACATTCTTCACTTTCCTTCACTTCCCATCAATTCTCTCTCGCAAACTAACAACTCAATCTCCTTATTCCACTAATACCTTTGATGTCTCGGCCTCCATTAACCAAGCCTTAAATGCCCTTTCCATCAAACCCAAACCCTTTCAAACGACTCACTCTAATTACCATTCAAGTTCACCTCTATCTCTCTCATTGCACCCTAGATTGACCGTTCATAACCCTTCCTACGAGGACTATGGTAGTCTTGTTAGAGCCCGACTTGCTCGTGGTGCCGCCCGAGTTCAATCCCTTAATAGAAAGCTTGAGCTCTCTTTAAAAGGGGGTAAACAATTTGGTAGAAGAATTAATGGATCTGATTCTACAAATTCACTTACTGTTCCCGTTACTTCAGGGGCGAGTCAAGGGGCTGGCGAATATTTTGCCCGGATTGGAGTCGGTCAACCTGTGCAATCGTATTTTTTTGTGCCTGATACTGGAAGTGATGTTTCGTGGCTACAATGTCAGCCATGTGATGGTGAGAATGGTTGTTATAAACAAATTGGCCCGATATTTGACCCGAAATCGTCGTCTTCTTATAGTCCACTGTCTTGTGATTCGGAACAATGTCATTTGTTGGATGAAGCTGCTTGTGATGTCAATTCGTGTATCTACGAAGTTGAATATGGTGATGGATCATTCACAGTTGGTGAACTTGCCACCGAAACGTTCTCATTTCGACATTCTAATTCCATCCCTAATCTCCCGATTGGTTGTGGTCATGACAATGAAGGCCTCTTTATTGGGGCTGCTGGTTTAATTGGCCTCGGTGGTGGGGCAATTTCCCTTTCTTCCCAATTAGAAGCGTCGTCATTTTCATATTGTCTGGTCGACCTAGACTCAGAATCATCCTCCACTCTTGACTTCAACGCAGACCAACCAAGCGACTCACTCACCTCCCCACTTGTGAAAAATGATCGATTCCCCACGTTTCGGTACGTGAAAGTAATTGGGATGAGCGTCGGCGGAAAACCTTTACCGATTTCCTCGTCAAGCTTTGAAATCGACGAATCAGGATCAGGGGGAATAATCGTGGATTCTGGGACGACTATAACTGAACTACCAAGTGATGTGTATGGCGTGTTAAGAGACGCGTTCGTGGGGCTAACAAAGAACCTCCCACCAGCACCAGGGGTATCACCGTTTGATACTTGTTATGACTTATCAAGTCAATCGAATGTGGAGGTACCAACCATAGCATTTATTTTGTCGGGGGAAAACTCGCTACAACTACCAGCAAAAAATTGTTTGATCCAAGTGGACTCAGCTGGAACTTTCTGCTTGGCGTTTCTTCCATCAACATTTCCACTTTCTATTATTGGGAATGTCCAACAGCAAGGAATACGTGTCAGCTATGACTTGGCCAACTCTCTCGTCGGATTCTCGACTGATAAATGTTAA

Protein sequence

MMNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQTTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGKQFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQPCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTVGELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCLVDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVPTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANSLVGFSTDKC*

Homology

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BLAST of Chy2G046400 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.1e-126
Identity = 244/493 (49.49%), Postives = 329/493 (66.73%), Query Frame = 0


Query: 13  LTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQTTHSN------ 72
           +T   FL      SR L+T  P  TN  DV +S+ Q    LS+ P     T +       
Sbjct: 13  VTLSLFLTTTDASSRSLST--PPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSD 72

Query: 73  ---YHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKG-GKQ 132
              ++SSSPLSL LH R T     ++DY SL  +RL R ++RV  +  K+  +++G  + 
Sbjct: 73  PVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRS 132

Query: 133 FGRRINGSDS---TNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQC 192
             + +   D+   T  LT PV SGASQG+GEYF+RIGVG P +  + V DTGSDV+W+QC
Sbjct: 133 DLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQC 192

Query: 193 QPCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFT 252
           +PC     CY+Q  P+F+P SSS+Y  L+C + QC LL+ +AC  N C+Y+V YGDGSFT
Sbjct: 193 EPC---ADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFT 252

Query: 253 VGELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYC 312
           VGELAT+T +F +S  I N+ +GCGHDNEGLF GAAGL+GLGGG +S+++Q++A+SFSYC
Sbjct: 253 VGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYC 312

Query: 313 LVDLDSESSSTLDFNADQ-PSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFE 372
           LVD DS  SS+LDFN+ Q      T+PL++N +  TF YV + G SVGG+ + +  + F+
Sbjct: 313 LVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFD 372

Query: 373 IDESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPP-APGVSPFDTCYDLSSQSN 432
           +D SGSGG+I+D GT +T L +  Y  LRDAF+ LT NL   +  +S FDTCYD SS S 
Sbjct: 373 VDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST 432

Query: 433 VEVPTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYD 491
           V+VPT+AF  +G  SL LPAKN LI VD +GTFC AF P++  LSIIGNVQQQG R++YD
Sbjct: 433 VKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYD 492

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BLAST of Chy2G046400 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.1e-97
Identity = 204/483 (42.24%), Postives = 280/483 (57.97%), Query Frame = 0


Query: 15  FFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQTTHSNYHSSSPLS 74
           FF FLH    L   L++ S  S   F +   +   L   +  P  F  TH +  SSS  +
Sbjct: 6   FFFFLH----LHLHLSSSSSISFPDFQIIDVLQPPLTVTATLP-DFNNTHFSDESSSKYT 65

Query: 75  LSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGKQFGRRINGSDS-- 134
           L L  R    + +Y ++   + AR+ R   RV ++ R++          G+ I  SDS  
Sbjct: 66  LRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRIS---------GKVIPSSDSRY 125

Query: 135 -TNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQPCDGENGCYKQ 194
             N     + SG  QG+GEYF RIGVG P +  + V D+GSD+ W+QCQPC     CYKQ
Sbjct: 126 EVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KLCYKQ 185

Query: 195 IGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTVGELATETFSFR 254
             P+FDP  S SY+ +SC S  C  ++ + C    C YEV YGDGS+T G LA ET +F 
Sbjct: 186 SDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFA 245

Query: 255 HSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLE---ASSFSYCLVDLDSESS 314
               + N+ +GCGH N G+FIGAAGL+G+GGG++S   QL      +F YCLV   ++S+
Sbjct: 246 -KTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDST 305

Query: 315 STLDFNADQ-PSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDESGSGGI 374
            +L F  +  P  +   PLV+N R P+F YV + G+ VGG  +P+    F++ E+G GG+
Sbjct: 306 GSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 365

Query: 375 IVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVPTIAFIL 434
           ++D+GT +T LP+  Y   RD F   T NLP A GVS FDTCYDLS   +V VPT++F  
Sbjct: 366 VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYF 425

Query: 435 SGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANSLVGFST 491
           +    L LPA+N L+ VD +GT+C AF  S   LSIIGN+QQ+GI+VS+D AN  VGF  
Sbjct: 426 TEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGP 470

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BLAST of Chy2G046400 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 6.0e-91
Identity = 209/492 (42.48%), Postives = 286/492 (58.13%), Query Frame = 0


Query: 9   AFLFLTFFTFLHFPSILS-RKLTTQSPYSTNTFDVS-ASINQALNALSIKPKPFQTTHSN 68
           A LF   F FL  PS  S     T  P S +    S  S     ++ S+    F+ + S+
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFE-SGSD 66

Query: 69  YHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGKQFGRR 128
             SSS ++L+L     + +    D   L  +RL R + RV+S      ++    +  GR 
Sbjct: 67  SESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKS------IATLAAQIPGRN 126

Query: 129 INGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQPCDGEN 188
           +  +      +  V SG SQG+GEYF R+GVG P +  + V DTGSD+ WLQC PC    
Sbjct: 127 VTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---R 186

Query: 189 GCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDV--NSCIYEVEYGDGSFTVGELA 248
            CY Q  PIFDP+ S +Y+ + C S  C  LD A C+    +C+Y+V YGDGSFTVG+ +
Sbjct: 187 RCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFS 246

Query: 249 TETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLE---ASSFSYCLV 308
           TET +FR  N +  + +GCGHDNEGLF+GAAGL+GLG G +S   Q        FSYCLV
Sbjct: 247 TETLTFRR-NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLV 306

Query: 309 D--LDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLP-ISSSSFE 368
           D    S+ SS +  NA     +  +PL+ N +  TF YV ++G+SVGG  +P +++S F+
Sbjct: 307 DRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFK 366

Query: 369 IDESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNV 428
           +D+ G+GG+I+DSGT++T L    Y  +RDAF    K L  AP  S FDTC+DLS+ + V
Sbjct: 367 LDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEV 426

Query: 429 EVPTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDL 488
           +VPT+     G + + LPA N LI VD+ G FC AF  +   LSIIGN+QQQG RV YDL
Sbjct: 427 KVPTVVLHFRGAD-VSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDL 484

Query: 489 ANSLVGFSTDKC 491
           A+S VGF+   C
Sbjct: 487 ASSRVGFAPGGC 484

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BLAST of Chy2G046400 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 9.2e-68
Identity = 155/397 (39.04%), Postives = 221/397 (55.67%), Query Frame = 0


Query: 101 RGAARVQSLNRKLELSLKGGKQFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQP 160
           +   + Q L R +E   +  ++    +NG          V +    G GEY   + +G P
Sbjct: 52  KNLTKFQLLERAIERGSRRLQRLEAMLNGPSG-------VETSVYAGDGEYLMNLSIGTP 111

Query: 161 VQSYFFVPDTGSDVSWLQCQPCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEA 220
            Q +  + DTGSD+ W QCQPC     C+ Q  PIF+P+ SSS+S L C S+ C  L   
Sbjct: 112 AQPFSAIMDTGSDLIWTQCQPC---TQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSP 171

Query: 221 ACDVNSCIYEVEYGDGSFTVGELATETFSFRHSNSIPNLPIGCGHDNEGLFIG-AAGLIG 280
            C  N C Y   YGDGS T G + TET +F  S SIPN+  GCG +N+G   G  AGL+G
Sbjct: 172 TCSNNFCQYTYGYGDGSETQGSMGTETLTF-GSVSIPNITFGCGENNQGFGQGNGAGLVG 231

Query: 281 LGGGAISLSSQLEASSFSYCLVDLDSESSSTLDFNADQPSDSLTSP---LVKNDRFPTFR 340
           +G G +SL SQL+ + FSYC+  + S + S L   +   S +  SP   L+++ + PTF 
Sbjct: 232 MGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFY 291

Query: 341 YVKVIGMSVGGKPLPISSSSFEID-ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTK 400
           Y+ + G+SVG   LPI  S+F ++  +G+GGII+DSGTT+T   ++ Y  +R  F+    
Sbjct: 292 YITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQI- 351

Query: 401 NLPPAPGVSP-FDTCYDL-SSQSNVEVPTIAFILSGENSLQLPAKNCLIQVDSAGTFCLA 460
           NLP   G S  FD C+   S  SN+++PT      G   L+LP++N  I   S G  CLA
Sbjct: 352 NLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDG-GDLELPSENYFIS-PSNGLICLA 411

Query: 461 FLPSTFPLSIIGNVQQQGIRVSYDLANSLVGFSTDKC 491
              S+  +SI GN+QQQ + V YD  NS+V F++ +C
Sbjct: 412 MGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Chy2G046400 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 9.2e-68
Identity = 159/404 (39.36%), Postives = 221/404 (54.70%), Query Frame = 0


Query: 94  LVRARLARGAARVQSLNRKLELSLKGGKQFGRRINGSDSTNSLTVPVTSGASQGAGEYFA 153
           L++  + RG  R++S+N  L+                 S++ +  PV +    G GEY  
Sbjct: 60  LIKRAIKRGERRMRSINAMLQ-----------------SSSGIETPVYA----GDGEYLM 119

Query: 154 RIGVGQPVQSYFFVPDTGSDVSWLQCQPCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQ 213
            + +G P  S+  + DTGSD+ W QC+PC     C+ Q  PIF+P+ SSS+S L C+S+ 
Sbjct: 120 NVAIGTPDSSFSAIMDTGSDLIWTQCEPC---TQCFSQPTPIFNPQDSSSFSTLPCESQY 179

Query: 214 CHLLDEAACDVNSCIYEVEYGDGSFTVGELATETFSFRHSNSIPNLPIGCGHDNEGLFIG 273
           C  L    C+ N C Y   YGDGS T G +ATETF+F  ++S+PN+  GCG DN+G   G
Sbjct: 180 CQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTF-ETSSVPNIAFGCGEDNQGFGQG 239

Query: 274 -AAGLIGLGGGAISLSSQLEASSFSYCLVDLDSESSSTLDFN---ADQPSDSLTSPLVKN 333
             AGLIG+G G +SL SQL    FSYC+    S S STL      +  P  S ++ L+ +
Sbjct: 240 NGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHS 299

Query: 334 DRFPTFRYVKVIGMSVGGKPLPISSSSFEIDESGSGGIIVDSGTTITELPSDVYGVLRDA 393
              PT+ Y+ + G++VGG  L I SS+F++ + G+GG+I+DSGTT+T LP D Y  +  A
Sbjct: 300 SLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQA 359

Query: 394 FVGLTKNLPPA-PGVSPFDTCYDLSSQ-SNVEVPTIAFILSGENSLQLPAKNCLIQVDSA 453
           F     NLP      S   TC+   S  S V+VP I+    G   L L  +N LI   + 
Sbjct: 360 FTDQI-NLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDG-GVLNLGEQNILIS-PAE 419

Query: 454 GTFCLAF-LPSTFPLSIIGNVQQQGIRVSYDLANSLVGFSTDKC 491
           G  CLA    S   +SI GN+QQQ  +V YDL N  V F   +C
Sbjct: 420 GVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Chy2G046400 vs. ExPASy TrEMBL
Match: A0A0A0LS14 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022480 PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.5e-274
Identity = 478/489 (97.75%), Postives = 481/489 (98.36%), Query Frame = 0


Query: 2   MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 61
           MNTSLSYAFLFLTFF  LHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ
Sbjct: 1   MNTSLSYAFLFLTFFASLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 60

Query: 62  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGK 121
           TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAAR QSLNRKLELSLKGGK
Sbjct: 61  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARAQSLNRKLELSLKGGK 120

Query: 122 QFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 181
           QFGRRINGSDSTNSLT PVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP
Sbjct: 121 QFGRRINGSDSTNSLTAPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 180

Query: 182 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTVG 241
           CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACD NSCIYEVEYGDGSFTVG
Sbjct: 181 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDANSCIYEVEYGDGSFTVG 240

Query: 242 ELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCLV 301
           ELATETFSFRHSNSIPNLPIGCGHDNEGLF+GAAGLIGLGGGAISLSSQLEA+SFSYCLV
Sbjct: 241 ELATETFSFRHSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLEATSFSYCLV 300

Query: 302 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 361
           DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE
Sbjct: 301 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 360

Query: 362 SGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 421
           SGSGGIIVDSGTTITE+PSDVY VLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP
Sbjct: 361 SGSGGIIVDSGTTITEIPSDVYDVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 420

Query: 422 TIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 481
           TIAFIL GENSLQLPAKNCL QVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS
Sbjct: 421 TIAFILPGENSLQLPAKNCLFQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 480

Query: 482 LVGFSTDKC 491
           LVGFSTDKC
Sbjct: 481 LVGFSTDKC 489

[Close]

BLAST of Chy2G046400 vs. ExPASy TrEMBL
Match: A0A5A7UQC3 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003680 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 4.0e-239
Identity = 428/490 (87.35%), Postives = 450/490 (91.84%), Query Frame = 0


Query: 1   MMNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYA LFLT FTFL FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGG 120
           Q THS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A RVQSLNRKLELSL G 
Sbjct: 61  Q-THS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGA 120

Query: 121 KQFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQ 180
           KQFG+RINGS STNSLT PVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+
Sbjct: 121 KQFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCK 180

Query: 181 PCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTV 240
           PC  EN C+KQ+ PIFDPKSSSSYS LSC+SEQC LLDEA C  NSCIYEVEYGDGSFT+
Sbjct: 181 PCANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTI 240

Query: 241 GELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCL 300
           GELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+ASSFSYCL
Sbjct: 241 GELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCL 300

Query: 301 VDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEID 360
           VDLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEID
Sbjct: 301 VDLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEID 360

Query: 361 ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEV 420
           ESGSGGIIVDSGTTIT+LPSDVY VLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEV
Sbjct: 361 ESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEV 420

Query: 421 PTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLAN 480
           P IAFIL G  SL+LPAKNCLIQVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL N
Sbjct: 421 PIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDN 480

Query: 481 SLVGFSTDKC 491
           S+VGF+T+KC
Sbjct: 481 SIVGFATNKC 488

[Close]

BLAST of Chy2G046400 vs. ExPASy TrEMBL
Match: A0A1S3BW42 (protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC103494116 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 4.0e-239
Identity = 428/490 (87.35%), Postives = 450/490 (91.84%), Query Frame = 0


Query: 1   MMNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYA LFLT FTFL FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGG 120
           Q THS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A RVQSLNRKLELSL G 
Sbjct: 61  Q-THS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGA 120

Query: 121 KQFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQ 180
           KQFG+RINGS STNSLT PVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+
Sbjct: 121 KQFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCK 180

Query: 181 PCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTV 240
           PC  EN C+KQ+ PIFDPKSSSSYS LSC+SEQC LLDEA C  NSCIYEVEYGDGSFT+
Sbjct: 181 PCANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTI 240

Query: 241 GELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCL 300
           GELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+ASSFSYCL
Sbjct: 241 GELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCL 300

Query: 301 VDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEID 360
           VDLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEID
Sbjct: 301 VDLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEID 360

Query: 361 ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEV 420
           ESGSGGIIVDSGTTIT+LPSDVY VLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEV
Sbjct: 361 ESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEV 420

Query: 421 PTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLAN 480
           P IAFIL G  SL+LPAKNCLIQVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL N
Sbjct: 421 PIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDN 480

Query: 481 SLVGFSTDKC 491
           S+VGF+T+KC
Sbjct: 481 SIVGFATNKC 488

[Close]

BLAST of Chy2G046400 vs. ExPASy TrEMBL
Match: A0A5D3DY88 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00360 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.2e-239
Identity = 428/490 (87.35%), Postives = 449/490 (91.63%), Query Frame = 0


Query: 1   MMNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYA LFLT FTFL FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGG 120
           Q THS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A RVQSLNRKLELSL G 
Sbjct: 61  Q-THS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGA 120

Query: 121 KQFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQ 180
           KQFG+RINGS STNSLT PVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+
Sbjct: 121 KQFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCK 180

Query: 181 PCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTV 240
           PC  EN C+KQ+ PIFDPK SSSYS LSCDSEQC LLDEA C  NSCIYEVEYGDGSFT+
Sbjct: 181 PCANENACFKQLDPIFDPKLSSSYSSLSCDSEQCQLLDEAGCSSNSCIYEVEYGDGSFTI 240

Query: 241 GELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCL 300
           GELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+ASSFSYCL
Sbjct: 241 GELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCL 300

Query: 301 VDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEID 360
           VDLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEID
Sbjct: 301 VDLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEID 360

Query: 361 ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEV 420
           ESGSGGIIVDSGTTIT+LPSDVY VLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEV
Sbjct: 361 ESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEV 420

Query: 421 PTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLAN 480
           P IAFIL G  SL+LPAKNCLIQVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL N
Sbjct: 421 PIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDN 480

Query: 481 SLVGFSTDKC 491
           S+VGF+T+KC
Sbjct: 481 SIVGFATNKC 488

[Close]

BLAST of Chy2G046400 vs. ExPASy TrEMBL
Match: A0A5A7UQC2 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003690 PE=4 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.4e-196
Identity = 364/490 (74.29%), Postives = 414/490 (84.49%), Query Frame = 0


Query: 2   MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 61
           M TSLS  FLFLT FT L F SILSRKL TQSPYST+ FDV AS NQALNALSIKPK  Q
Sbjct: 1   MKTSLSSVFLFLTIFTSLQFSSILSRKL-TQSPYSTSIFDVLASTNQALNALSIKPKHLQ 60

Query: 62  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGK 121
            THS+  +SS LSL L+PRL++HNPSY+DY SLVRARLAR AARVQ LNR LE SL GGK
Sbjct: 61  -THSHLPNSS-LSLPLYPRLSLHNPSYKDYDSLVRARLARDAARVQFLNRNLEHSLNGGK 120

Query: 122 QFGRRINGSDSTNSLTVPVTSGASQGAG-EYFARIGVGQPVQSYFFVPDTGSDVSWLQCQ 181
            FG   NGS   +S+T PV SG S+G+G EY A++GVGQPV+ ++ VPDTGSDV+WLQCQ
Sbjct: 121 DFGEVTNGSLIGDSITAPVVSGQSKGSGAEYLAQVGVGQPVKLFYLVPDTGSDVTWLQCQ 180

Query: 182 PCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTV 241
           PC  EN CYKQI PIFDPKSSSSY+PLSC+S+QC LLD   C+  +CIY+V YGDGSFT 
Sbjct: 181 PCATENACYKQIDPIFDPKSSSSYTPLSCNSQQCGLLDRPNCNSGTCIYQVHYGDGSFTT 240

Query: 242 GELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCL 301
           GELATET SF +SNSIPNLPIGCGHDNEGLF G AGLIGLGGGAISLSSQL+ASSFSYCL
Sbjct: 241 GELATETLSFGNSNSIPNLPIGCGHDNEGLFAGGAGLIGLGGGAISLSSQLKASSFSYCL 300

Query: 302 VDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEID 361
           V++DS+SSSTL+FN++ PSDSLTSPLVKNDRF ++RYVKVIG+SVGGK LPISS+ FEID
Sbjct: 301 VNMDSDSSSTLEFNSNMPSDSLTSPLVKNDRFHSYRYVKVIGISVGGKTLPISSTRFEID 360

Query: 362 ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEV 421
           ESG GGIIVDSGT I+ LPSDVY  LR+AFV LT +L PAPG+S FDTCY+LSSQSNVEV
Sbjct: 361 ESGLGGIIVDSGTIISRLPSDVYESLREAFVKLTSSLSPAPGISVFDTCYNLSSQSNVEV 420

Query: 422 PTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLAN 481
           PTIAF+LSG  SL+LPA+N LI+VD+AGT+CLAF+ +   LSIIG+ QQQGIRVSYDL N
Sbjct: 421 PTIAFVLSGGTSLRLPARNYLIRVDTAGTYCLAFIKTKSSLSIIGSFQQQGIRVSYDLTN 480

Query: 482 SLVGFSTDKC 491
           SLVGFST+KC
Sbjct: 481 SLVGFSTNKC 487

[Close]

BLAST of Chy2G046400 vs. NCBI nr
Match: KGN63815.2 (hypothetical protein Csa_013944 [Cucumis sativus])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 480/489 (98.16%), Postives = 483/489 (98.77%), Query Frame = 0


Query: 2   MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 61
           MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ
Sbjct: 1   MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 60

Query: 62  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGK 121
           TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAAR QSLNRKLELSLKGGK
Sbjct: 61  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARAQSLNRKLELSLKGGK 120

Query: 122 QFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 181
           QFGRRINGSDSTNSLT PVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP
Sbjct: 121 QFGRRINGSDSTNSLTAPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 180

Query: 182 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTVG 241
           CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACD NSCIYEVEYGDGSFTVG
Sbjct: 181 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDANSCIYEVEYGDGSFTVG 240

Query: 242 ELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCLV 301
           ELATETFSFRHSNSIPNLPIGCGHDNEGLF+GAAGLIGLGGGAISLSSQLEA+SFSYCLV
Sbjct: 241 ELATETFSFRHSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLEATSFSYCLV 300

Query: 302 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 361
           DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE
Sbjct: 301 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 360

Query: 362 SGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 421
           SGSGGIIVDSGTTITE+PSDVY VLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP
Sbjct: 361 SGSGGIIVDSGTTITEIPSDVYDVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 420

Query: 422 TIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 481
           TIAFIL GENSLQLPAKNCL QVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS
Sbjct: 421 TIAFILPGENSLQLPAKNCLFQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 480

Query: 482 LVGFSTDKC 490
           LVGFSTDKC
Sbjct: 481 LVGFSTDKC 489

[Close]

BLAST of Chy2G046400 vs. NCBI nr
Match: XP_004138237.3 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 480/489 (98.16%), Postives = 483/489 (98.77%), Query Frame = 0


Query: 2   MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 61
           MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ
Sbjct: 35  MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 94

Query: 62  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGK 121
           TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAAR QSLNRKLELSLKGGK
Sbjct: 95  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARAQSLNRKLELSLKGGK 154

Query: 122 QFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 181
           QFGRRINGSDSTNSLT PVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP
Sbjct: 155 QFGRRINGSDSTNSLTAPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 214

Query: 182 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTVG 241
           CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACD NSCIYEVEYGDGSFTVG
Sbjct: 215 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDANSCIYEVEYGDGSFTVG 274

Query: 242 ELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCLV 301
           ELATETFSFRHSNSIPNLPIGCGHDNEGLF+GAAGLIGLGGGAISLSSQLEA+SFSYCLV
Sbjct: 275 ELATETFSFRHSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLEATSFSYCLV 334

Query: 302 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 361
           DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE
Sbjct: 335 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 394

Query: 362 SGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 421
           SGSGGIIVDSGTTITE+PSDVY VLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP
Sbjct: 395 SGSGGIIVDSGTTITEIPSDVYDVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 454

Query: 422 TIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 481
           TIAFIL GENSLQLPAKNCL QVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS
Sbjct: 455 TIAFILPGENSLQLPAKNCLFQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 514

Query: 482 LVGFSTDKC 490
           LVGFSTDKC
Sbjct: 515 LVGFSTDKC 523

[Close]

BLAST of Chy2G046400 vs. NCBI nr
Match: XP_008453383.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo] >KAA0058062.1 protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo var. makuwa])

HSP 1 Score: 837 bits (2162), Expect = 1.02e-303
Identity = 428/490 (87.35%), Postives = 450/490 (91.84%), Query Frame = 0


Query: 1   MMNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYA LFLT FTFL FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGG 120
           QT HS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A RVQSLNRKLELSL G 
Sbjct: 61  QT-HS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGA 120

Query: 121 KQFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQ 180
           KQFG+RINGS STNSLT PVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+
Sbjct: 121 KQFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCK 180

Query: 181 PCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTV 240
           PC  EN C+KQ+ PIFDPKSSSSYS LSC+SEQC LLDEA C  NSCIYEVEYGDGSFT+
Sbjct: 181 PCANENACFKQLDPIFDPKSSSSYSSLSCNSEQCQLLDEAGCSSNSCIYEVEYGDGSFTI 240

Query: 241 GELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCL 300
           GELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+ASSFSYCL
Sbjct: 241 GELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCL 300

Query: 301 VDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEID 360
           VDLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEID
Sbjct: 301 VDLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEID 360

Query: 361 ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEV 420
           ESGSGGIIVDSGTTIT+LPSDVY VLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEV
Sbjct: 361 ESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEV 420

Query: 421 PTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLAN 480
           P IAFIL G  SL+LPAKNCLIQVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL N
Sbjct: 421 PIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDN 480

Query: 481 SLVGFSTDKC 490
           S+VGF+T+KC
Sbjct: 481 SIVGFATNKC 488

[Close]

BLAST of Chy2G046400 vs. NCBI nr
Match: TYK28408.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo var. makuwa])

HSP 1 Score: 837 bits (2161), Expect = 1.45e-303
Identity = 428/490 (87.35%), Postives = 449/490 (91.63%), Query Frame = 0


Query: 1   MMNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPF 60
           MMNTSLSYA LFLT FTFL FPSILSRKLT QSPYST TFDVSASINQALNALSIKPKPF
Sbjct: 1   MMNTSLSYALLFLTIFTFLQFPSILSRKLTAQSPYSTTTFDVSASINQALNALSIKPKPF 60

Query: 61  QTTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGG 120
           QT HS YHS+SPLSLSLHPRLTVHNPSY+DYG+LVRARLAR A RVQSLNRKLELSL G 
Sbjct: 61  QT-HS-YHSNSPLSLSLHPRLTVHNPSYKDYGTLVRARLARHATRVQSLNRKLELSLNGA 120

Query: 121 KQFGRRINGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQ 180
           KQFG+RINGS STNSLT PVTSGAS G GEYFARIGVGQPVQS+F VPDTGSDV+WLQC+
Sbjct: 121 KQFGKRINGSASTNSLTAPVTSGASHGDGEYFARIGVGQPVQSFFLVPDTGSDVTWLQCK 180

Query: 181 PCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTV 240
           PC  EN C+KQ+ PIFDPK SSSYS LSCDSEQC LLDEA C  NSCIYEVEYGDGSFT+
Sbjct: 181 PCANENACFKQLDPIFDPKLSSSYSSLSCDSEQCQLLDEAGCSSNSCIYEVEYGDGSFTI 240

Query: 241 GELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCL 300
           GELATET SF +SNSIPNLPIGCGHDNEGLF  AAGLIGLGGGAISLSSQL+ASSFSYCL
Sbjct: 241 GELATETLSFGNSNSIPNLPIGCGHDNEGLFDAAAGLIGLGGGAISLSSQLQASSFSYCL 300

Query: 301 VDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEID 360
           VDLDS+SSSTLDFNADQPSDSLTSPLVKN+RFP+FRYVKVIGMSVGGK LPISSS FEID
Sbjct: 301 VDLDSDSSSTLDFNADQPSDSLTSPLVKNNRFPSFRYVKVIGMSVGGKRLPISSSRFEID 360

Query: 361 ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEV 420
           ESGSGGIIVDSGTTIT+LPSDVY VLRDAFVGLT NLP APGVSPFDTCYDLSSQS+VEV
Sbjct: 361 ESGSGGIIVDSGTTITQLPSDVYDVLRDAFVGLTTNLPTAPGVSPFDTCYDLSSQSSVEV 420

Query: 421 PTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLAN 480
           P IAFIL G  SL+LPAKNCLIQVDSAGTFCLAFLP TFPLSIIGNVQQQGIRVSYDL N
Sbjct: 421 PIIAFILPGGKSLKLPAKNCLIQVDSAGTFCLAFLPGTFPLSIIGNVQQQGIRVSYDLDN 480

Query: 481 SLVGFSTDKC 490
           S+VGF+T+KC
Sbjct: 481 SIVGFATNKC 488

[Close]

BLAST of Chy2G046400 vs. NCBI nr
Match: XP_038878113.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida])

HSP 1 Score: 727 bits (1877), Expect = 2.39e-260
Identity = 376/490 (76.73%), Postives = 419/490 (85.51%), Query Frame = 0


Query: 2   MNTSLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQ 61
           MN S+S AF+FLT  T L FPSI SRKLT QSPYST  FDVSAS  QA NALSIKPKPF+
Sbjct: 1   MNISISSAFIFLTILTSLQFPSIFSRKLT-QSPYSTAIFDVSASTKQAQNALSIKPKPFE 60

Query: 62  TTHSNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGK 121
           T HS +H +SPLSL LHPRLT++NPSY+DYGSLVRARLAR A RVQSLNR LELSL GG+
Sbjct: 61  T-HS-HHPNSPLSLPLHPRLTLYNPSYKDYGSLVRARLARDAVRVQSLNRNLELSLNGGQ 120

Query: 122 QFGRRINGSDSTNSLTVPVTSGASQG-AGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQ 181
           + G  INGS+S +S+T PV SG S G  GEYFARIGVGQPVQS++ VPDTGSDV+WLQCQ
Sbjct: 121 KIGAGINGSESADSITAPVVSGQSLGNGGEYFARIGVGQPVQSFYLVPDTGSDVTWLQCQ 180

Query: 182 PCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTV 241
           PC  E  CYKQI PIFDPKSSSSY+ LSC+S+QC LLD+A C+ + C Y+V YGDGSFT 
Sbjct: 181 PCAAEKACYKQIDPIFDPKSSSSYTALSCNSQQCQLLDKANCNSDVCQYQVNYGDGSFTT 240

Query: 242 GELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCL 301
           GELATET SF +SNSIPNLPIGCGHDNEGLF+GAAGLIGLGGGAISLSSQL+ASSFSYCL
Sbjct: 241 GELATETLSFGNSNSIPNLPIGCGHDNEGLFVGAAGLIGLGGGAISLSSQLKASSFSYCL 300

Query: 302 VDLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEID 361
           VDLDS+SSSTL+FN D+PSDSLTSPLVKNDRF ++RYVKV+GMSVGG PLPISS+ FEID
Sbjct: 301 VDLDSDSSSTLEFNTDRPSDSLTSPLVKNDRFHSYRYVKVVGMSVGGNPLPISSTRFEID 360

Query: 362 ESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEV 421
           ESG GGIIVDSGTTIT+LPSDVY  LR+AFV  T NLPPA G+S FDTCYDLS QS+VEV
Sbjct: 361 ESGLGGIIVDSGTTITQLPSDVYESLREAFVKQTSNLPPAQGISLFDTCYDLSGQSSVEV 420

Query: 422 PTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLAN 481
           P IAF+L GENSL+LPAKN LI VDS GT+CLAF  +   LSIIG+ QQQGIRVSYDLAN
Sbjct: 421 PVIAFVLPGENSLRLPAKNYLIPVDSDGTYCLAFFKTRSSLSIIGSFQQQGIRVSYDLAN 480

Query: 482 SLVGFSTDKC 490
           SLVGFST+KC
Sbjct: 481 SLVGFSTNKC 487

[Close]

BLAST of Chy2G046400 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 463.0 bits (1190), Expect = 3.0e-130
Identity = 247/489 (50.51%), Postives = 333/489 (68.10%), Query Frame = 0


Query: 5   SLSYAFLFLTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQTTH 64
           S +Y+F F  FF   H  S+ SR L   S  +T+  +V+ SI++     S +    Q   
Sbjct: 2   SPNYSFFFFIFFLTSH-SSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLN--QQEE 61

Query: 65  SNYHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKG-GKQF 124
             + +SS  SL LH R++V    + DY SL  ARL R  ARV+SL  +L+L++    K  
Sbjct: 62  QTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKAD 121

Query: 125 GRRINGSDST--NSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQP 184
            + I+   +T    +  P+ SG +QG+GEYF R+G+G+P +  + V DTGSDV+WLQC P
Sbjct: 122 LKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTP 181

Query: 185 CDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTVG 244
           C     CY Q  PIF+P SSSSY PLSCD+ QC+ L+ + C   +C+YEV YGDGS+TVG
Sbjct: 182 C---ADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVG 241

Query: 245 ELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYCLV 304
           + ATET +   S  + N+ +GCGH NEGLF+GAAGL+GLGGG ++L SQL  +SFSYCLV
Sbjct: 242 DFATETLTI-GSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLV 301

Query: 305 DLDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDE 364
           D DS+S+ST+DF      D++ +PL++N +  TF Y+ + G+SVGG+ L I  SSFE+DE
Sbjct: 302 DRDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDE 361

Query: 365 SGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVP 424
           SGSGGII+DSGT +T L +++Y  LRD+FV  T +L  A GV+ FDTCY+LS+++ VEVP
Sbjct: 362 SGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVP 421

Query: 425 TIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANS 484
           T+AF   G   L LPAKN +I VDS GTFCLAF P+   L+IIGNVQQQG RV++DLANS
Sbjct: 422 TVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANS 481

Query: 485 LVGFSTDKC 491
           L+GFS++KC
Sbjct: 482 LIGFSSNKC 483

[Close]

BLAST of Chy2G046400 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 454.9 bits (1169), Expect = 8.2e-128
Identity = 244/493 (49.49%), Postives = 329/493 (66.73%), Query Frame = 0


Query: 13  LTFFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQTTHSN------ 72
           +T   FL      SR L+T  P  TN  DV +S+ Q    LS+ P     T +       
Sbjct: 13  VTLSLFLTTTDASSRSLST--PPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSD 72

Query: 73  ---YHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKG-GKQ 132
              ++SSSPLSL LH R T     ++DY SL  +RL R ++RV  +  K+  +++G  + 
Sbjct: 73  PVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRS 132

Query: 133 FGRRINGSDS---TNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQC 192
             + +   D+   T  LT PV SGASQG+GEYF+RIGVG P +  + V DTGSDV+W+QC
Sbjct: 133 DLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQC 192

Query: 193 QPCDGENGCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFT 252
           +PC     CY+Q  P+F+P SSS+Y  L+C + QC LL+ +AC  N C+Y+V YGDGSFT
Sbjct: 193 EPC---ADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFT 252

Query: 253 VGELATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLEASSFSYC 312
           VGELAT+T +F +S  I N+ +GCGHDNEGLF GAAGL+GLGGG +S+++Q++A+SFSYC
Sbjct: 253 VGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYC 312

Query: 313 LVDLDSESSSTLDFNADQ-PSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFE 372
           LVD DS  SS+LDFN+ Q      T+PL++N +  TF YV + G SVGG+ + +  + F+
Sbjct: 313 LVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFD 372

Query: 373 IDESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPP-APGVSPFDTCYDLSSQSN 432
           +D SGSGG+I+D GT +T L +  Y  LRDAF+ LT NL   +  +S FDTCYD SS S 
Sbjct: 373 VDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST 432

Query: 433 VEVPTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYD 491
           V+VPT+AF  +G  SL LPAKN LI VD +GTFC AF P++  LSIIGNVQQQG R++YD
Sbjct: 433 VKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYD 492

[Close]

BLAST of Chy2G046400 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 358.6 bits (919), Expect = 8.0e-99
Identity = 204/483 (42.24%), Postives = 280/483 (57.97%), Query Frame = 0


Query: 15  FFTFLHFPSILSRKLTTQSPYSTNTFDVSASINQALNALSIKPKPFQTTHSNYHSSSPLS 74
           FF FLH    L   L++ S  S   F +   +   L   +  P  F  TH +  SSS  +
Sbjct: 6   FFFFLH----LHLHLSSSSSISFPDFQIIDVLQPPLTVTATLP-DFNNTHFSDESSSKYT 65

Query: 75  LSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGKQFGRRINGSDS-- 134
           L L  R    + +Y ++   + AR+ R   RV ++ R++          G+ I  SDS  
Sbjct: 66  LRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRIS---------GKVIPSSDSRY 125

Query: 135 -TNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQPCDGENGCYKQ 194
             N     + SG  QG+GEYF RIGVG P +  + V D+GSD+ W+QCQPC     CYKQ
Sbjct: 126 EVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPC---KLCYKQ 185

Query: 195 IGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDVNSCIYEVEYGDGSFTVGELATETFSFR 254
             P+FDP  S SY+ +SC S  C  ++ + C    C YEV YGDGS+T G LA ET +F 
Sbjct: 186 SDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFA 245

Query: 255 HSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLE---ASSFSYCLVDLDSESS 314
               + N+ +GCGH N G+FIGAAGL+G+GGG++S   QL      +F YCLV   ++S+
Sbjct: 246 -KTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDST 305

Query: 315 STLDFNADQ-PSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLPISSSSFEIDESGSGGI 374
            +L F  +  P  +   PLV+N R P+F YV + G+ VGG  +P+    F++ E+G GG+
Sbjct: 306 GSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 365

Query: 375 IVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNVEVPTIAFIL 434
           ++D+GT +T LP+  Y   RD F   T NLP A GVS FDTCYDLS   +V VPT++F  
Sbjct: 366 VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYF 425

Query: 435 SGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDLANSLVGFST 491
           +    L LPA+N L+ VD +GT+C AF  S   LSIIGN+QQ+GI+VS+D AN  VGF  
Sbjct: 426 TEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGP 470

[Close]

BLAST of Chy2G046400 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 341.7 bits (875), Expect = 1.0e-93
Identity = 200/439 (45.56%), Postives = 267/439 (60.82%), Query Frame = 0


Query: 69  SSSPLSLSLHPRLTVHNPSYEDY--GSLVRARLARGAARVQSLNRKLELSLKGGKQFGRR 128
           S S  SLS+H        S+ D     L   RL R + RV+S+     +S   G+   +R
Sbjct: 55  SESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVST--GRNATKR 114

Query: 129 INGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQPCDGEN 188
                +    +  V SG SQG+GEYF R+GVG P  + + V DTGSDV WLQC PC    
Sbjct: 115 T--PRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPC---K 174

Query: 189 GCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDV----NSCIYEVEYGDGSFTVGE 248
            CY Q   IFDPK S +++ + C S  C  LD+++  V     +C+Y+V YGDGSFT G+
Sbjct: 175 ACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGD 234

Query: 249 LATETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLE---ASSFSYC 308
            +TET +F H   + ++P+GCGHDNEGLF+GAAGL+GLG G +S  SQ +      FSYC
Sbjct: 235 FSTETLTF-HGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYC 294

Query: 309 LVDLDSESS-----STLDF-NADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLP-I 368
           LVD  S  S     ST+ F NA  P  S+ +PL+ N +  TF Y++++G+SVGG  +P +
Sbjct: 295 LVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGV 354

Query: 369 SSSSFEIDESGSGGIIVDSGTTITELPSDVYGVLRDAF-VGLTKNLPPAPGVSPFDTCYD 428
           S S F++D +G+GG+I+DSGT++T L    Y  LRDAF +G TK L  AP  S FDTC+D
Sbjct: 355 SESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATK-LKRAPSYSLFDTCFD 414

Query: 429 LSSQSNVEVPTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQG 488
           LS  + V+VPT+ F   G   + LPA N LI V++ G FC AF  +   LSIIGN+QQQG
Sbjct: 415 LSGMTTVKVPTVVFHFGG-GEVSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQG 474

Query: 489 IRVSYDLANSLVGFSTDKC 491
            RV+YDL  S VGF +  C
Sbjct: 475 FRVAYDLVGSRVGFLSRAC 483

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BLAST of Chy2G046400 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 336.3 bits (861), Expect = 4.2e-92
Identity = 209/492 (42.48%), Postives = 286/492 (58.13%), Query Frame = 0


Query: 9   AFLFLTFFTFLHFPSILS-RKLTTQSPYSTNTFDVS-ASINQALNALSIKPKPFQTTHSN 68
           A LF   F FL  PS  S     T  P S +    S  S     ++ S+    F+ + S+
Sbjct: 7   ALLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFE-SGSD 66

Query: 69  YHSSSPLSLSLHPRLTVHNPSYEDYGSLVRARLARGAARVQSLNRKLELSLKGGKQFGRR 128
             SSS ++L+L     + +    D   L  +RL R + RV+S      ++    +  GR 
Sbjct: 67  SESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKS------IATLAAQIPGRN 126

Query: 129 INGSDSTNSLTVPVTSGASQGAGEYFARIGVGQPVQSYFFVPDTGSDVSWLQCQPCDGEN 188
           +  +      +  V SG SQG+GEYF R+GVG P +  + V DTGSD+ WLQC PC    
Sbjct: 127 VTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC---R 186

Query: 189 GCYKQIGPIFDPKSSSSYSPLSCDSEQCHLLDEAACDV--NSCIYEVEYGDGSFTVGELA 248
            CY Q  PIFDP+ S +Y+ + C S  C  LD A C+    +C+Y+V YGDGSFTVG+ +
Sbjct: 187 RCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFS 246

Query: 249 TETFSFRHSNSIPNLPIGCGHDNEGLFIGAAGLIGLGGGAISLSSQLE---ASSFSYCLV 308
           TET +FR  N +  + +GCGHDNEGLF+GAAGL+GLG G +S   Q        FSYCLV
Sbjct: 247 TETLTFRR-NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLV 306

Query: 309 D--LDSESSSTLDFNADQPSDSLTSPLVKNDRFPTFRYVKVIGMSVGGKPLP-ISSSSFE 368
           D    S+ SS +  NA     +  +PL+ N +  TF YV ++G+SVGG  +P +++S F+
Sbjct: 307 DRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFK 366

Query: 369 IDESGSGGIIVDSGTTITELPSDVYGVLRDAFVGLTKNLPPAPGVSPFDTCYDLSSQSNV 428
           +D+ G+GG+I+DSGT++T L    Y  +RDAF    K L  AP  S FDTC+DLS+ + V
Sbjct: 367 LDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEV 426

Query: 429 EVPTIAFILSGENSLQLPAKNCLIQVDSAGTFCLAFLPSTFPLSIIGNVQQQGIRVSYDL 488
           +VPT+     G + + LPA N LI VD+ G FC AF  +   LSIIGN+QQQG RV YDL
Sbjct: 427 KVPTVVLHFRGAD-VSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDL 484

Query: 489 ANSLVGFSTDKC 491
           A+S VGF+   C
Sbjct: 487 ASSRVGFAPGGC 484

The following BLAST results are available for this feature:

BLAST of Chy2G046400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	1.1e-126	49.49	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LHE3	1.1e-97	42.24	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	6.0e-91	42.48	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	9.2e-68	39.04	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	9.2e-68	39.36	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Chy2G046400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LS14	6.5e-274	97.75	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G02248...	[more]
A0A5A7UQC3	4.0e-239	87.35	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BW42	4.0e-239	87.35	protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo OX=3656 GN=LOC10349411...	[more]
A0A5D3DY88	5.2e-239	87.35	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A5A7UQC2	2.4e-196	74.29	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]

BLAST of Chy2G046400 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN63815.2	0.0	98.16	hypothetical protein Csa_013944 [Cucumis sativus]	[more]
XP_004138237.3	0.0	98.16	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis sativus]	[more]
XP_008453383.1	1.02e-303	87.35	PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo] >KAA0058062....	[more]
TYK28408.1	1.45e-303	87.35	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Cucumis melo var. makuwa]	[more]
XP_038878113.1	2.39e-260	76.73	protein ASPARTIC PROTEASE IN GUARD CELL 1 [Benincasa hispida]	[more]

BLAST of Chy2G046400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G25510.1	3.0e-130	50.51	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	8.2e-128	49.49	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	8.0e-99	42.24	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.0e-93	45.56	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	4.2e-92	42.48	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 337..486 e-value: 2.1E-36 score: 125.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 125..312 e-value: 7.5E-48 score: 165.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 313..490 e-value: 4.3E-50 score: 171.9
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 146..490
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 151..311 e-value: 3.4E-45 score: 154.4
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 14..490
None	No IPR available	PANTHER	PTHR13683:SF274	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1	coord: 14..490
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 151..486 score: 42.507488

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy2G046400.1	Chy2G046400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity