Chy2G045110 (gene) Cucumber (hystrix) v1

Overview
NameChy2G045110
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionIsocitrate dehydrogenase [NADP]
LocationchrH02: 26554908 .. 26555090 (+)
RNA-Seq ExpressionChy2G045110
SyntenyChy2G045110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGGTATTGTCTTCAAAGAACCGATTCTTCGCAGAAATGTTCCTCGCCTTGTACCAGGTTGGACGAAGCCTATTTGTATTGGAAGACATGCTTTTGGTGATCAATACAGAGCTGCTACTGATACAGTCATCAGACGACCTGGGAAATTGAAATTTGTTTTTGAGGGGCAGGGGACGTAA

mRNA sequence

ATGAATGGTATTGTCTTCAAAGAACCGATTCTTCGCAGAAATGTTCCTCGCCTTGTACCAGGTTGGACGAAGCCTATTTGTATTGGAAGACATGCTTTTGGTGATCAATACAGAGCTGCTACTGATACAGTCATCAGACGACCTGGGAAATTGAAATTTGTTTTTGAGGGGCAGGGGACGTAA

Coding sequence (CDS)

ATGAATGGTATTGTCTTCAAAGAACCGATTCTTCGCAGAAATGTTCCTCGCCTTGTACCAGGTTGGACGAAGCCTATTTGTATTGGAAGACATGCTTTTGGTGATCAATACAGAGCTGCTACTGATACAGTCATCAGACGACCTGGGAAATTGAAATTTGTTTTTGAGGGGCAGGGGACGTAA

Protein sequence

MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT*
Homology
BLAST of Chy2G045110 vs. ExPASy Swiss-Prot
Match: Q9SLK0 (Peroxisomal isocitrate dehydrogenase [NADP] OS=Arabidopsis thaliana OX=3702 GN=ICDH PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 6.2e-22
Identity = 44/60 (73.33%), Postives = 51/60 (85.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPI+ RN+PRLVPGWTKPICIGRHAFGDQYR ATD ++  PGKLK VFE  G+
Sbjct: 105 LNGTVFREPIICRNIPRLVPGWTKPICIGRHAFGDQYR-ATDLIVNEPGKLKLVFEPSGS 163

BLAST of Chy2G045110 vs. ExPASy Swiss-Prot
Match: Q40345 (Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 8.1e-22
Identity = 46/61 (75.41%), Postives = 54/61 (88.52%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVF--EGQ 60
           +NG VF+EPI+ +N+PRL+PGWTKPICIGRHAFGDQYR ATD+VI+ PGKLK VF  EGQ
Sbjct: 126 LNGTVFREPIICKNIPRLIPGWTKPICIGRHAFGDQYR-ATDSVIKGPGKLKLVFVPEGQ 185

BLAST of Chy2G045110 vs. ExPASy Swiss-Prot
Match: P50218 (Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.3e-21
Identity = 45/60 (75.00%), Postives = 52/60 (86.67%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPI+ +N+PRLVPGWTKPICIGRHAFGDQYR ATDTVI+  GKLK VF  +GT
Sbjct: 105 LNGTVFREPIMCKNIPRLVPGWTKPICIGRHAFGDQYR-ATDTVIQGAGKLKLVFVPEGT 163

BLAST of Chy2G045110 vs. ExPASy Swiss-Prot
Match: P50217 (Isocitrate dehydrogenase [NADP] OS=Solanum tuberosum OX=4113 GN=ICDH-1 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.2e-21
Identity = 44/60 (73.33%), Postives = 52/60 (86.67%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPI+ +N+PRLVPGWTKPICIGRHAFGDQYR ATDTVI+  GKLK VF  +G+
Sbjct: 105 LNGTVFREPIMCKNIPRLVPGWTKPICIGRHAFGDQYR-ATDTVIKGAGKLKLVFVPEGS 163

BLAST of Chy2G045110 vs. ExPASy Swiss-Prot
Match: Q9SRZ6 (Cytosolic isocitrate dehydrogenase [NADP] OS=Arabidopsis thaliana OX=3702 GN=CICDH PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 8.9e-21
Identity = 43/58 (74.14%), Postives = 50/58 (86.21%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQ 59
           +NG VF+EPI+ +NVP+LVPGWTKPICIGRHAFGDQYR ATD VI+ PGKL   FEG+
Sbjct: 105 LNGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYR-ATDAVIKGPGKLTMTFEGK 161

BLAST of Chy2G045110 vs. ExPASy TrEMBL
Match: A0A0A0KRB4 (Isocitrate dehydrogenase [NADP] OS=Cucumis sativus OX=3659 GN=Csa_5G346550 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 51/60 (85.00%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYR ATDTVIR PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYR-ATDTVIRGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. ExPASy TrEMBL
Match: Q8RW69 (Isocitrate dehydrogenase [NADP] OS=Cucumis sativus OX=3659 GN=IDH PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 51/60 (85.00%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYR ATDTVIR PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYR-ATDTVIRGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. ExPASy TrEMBL
Match: A0A1S4E429 (Isocitrate dehydrogenase [NADP] OS=Cucumis melo OX=3656 GN=LOC103501355 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.4e-21
Identity = 50/60 (83.33%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYR ATDTVI+ PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYR-ATDTVIKGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. ExPASy TrEMBL
Match: A0A6J1FJA0 (Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111444771 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.4e-21
Identity = 50/60 (83.33%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYR ATDTVI+ PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYR-ATDTVIKGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. ExPASy TrEMBL
Match: A0A5A7VEU1 (Isocitrate dehydrogenase [NADP] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold871G00180 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.4e-21
Identity = 50/60 (83.33%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYR ATDTVI+ PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYR-ATDTVIKGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. NCBI nr
Match: NP_001295854.1 (cytosolic isocitrate dehydrogenase [Cucumis sativus] >CAD24779.1 isocitrate dehydrogenase [Cucumis sativus])

HSP 1 Score: 109 bits (273), Expect = 1.42e-26
Identity = 51/60 (85.00%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYRA TDTVIR PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYRA-TDTVIRGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. NCBI nr
Match: XP_011655148.1 (cytosolic isocitrate dehydrogenase isoform X2 [Cucumis sativus] >XP_031740811.1 cytosolic isocitrate dehydrogenase isoform X2 [Cucumis sativus] >KAE8648364.1 hypothetical protein Csa_023086 [Cucumis sativus])

HSP 1 Score: 109 bits (273), Expect = 1.42e-26
Identity = 51/60 (85.00%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYRA TDTVIR PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYRA-TDTVIRGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. NCBI nr
Match: XP_031740810.1 (cytosolic isocitrate dehydrogenase isoform X1 [Cucumis sativus])

HSP 1 Score: 109 bits (273), Expect = 1.46e-26
Identity = 51/60 (85.00%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYRA TDTVIR PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYRA-TDTVIRGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. NCBI nr
Match: KAG1367693.1 (putative Isocitrate dehydrogenase [NADP] [Cocos nucifera])

HSP 1 Score: 104 bits (260), Expect = 3.60e-26
Identity = 46/58 (79.31%), Postives = 53/58 (91.38%), Query Frame = 0

Query: 1  MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQ 58
          +NG VF+EPI+ +N+PRLVPGWTKPICIGRHAFGDQYRA TDTVI+ PGKLK VFEG+
Sbjct: 27 LNGTVFREPIICKNIPRLVPGWTKPICIGRHAFGDQYRA-TDTVIKGPGKLKLVFEGK 83

BLAST of Chy2G045110 vs. NCBI nr
Match: KAG6579052.1 (Cytosolic isocitrate dehydrogenase [NADP], partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 108 bits (270), Expect = 3.84e-26
Identity = 50/60 (83.33%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPIL +NVPRLVPGWTKPICIGRHAFGDQYRA TDTVI+ PGKLK VFEGQ T
Sbjct: 105 LNGTVFREPILCKNVPRLVPGWTKPICIGRHAFGDQYRA-TDTVIKGPGKLKLVFEGQET 163

BLAST of Chy2G045110 vs. TAIR 10
Match: AT1G54340.1 (isocitrate dehydrogenase )

HSP 1 Score: 104.0 bits (258), Expect = 4.4e-23
Identity = 44/60 (73.33%), Postives = 51/60 (85.00%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQGT 60
           +NG VF+EPI+ RN+PRLVPGWTKPICIGRHAFGDQYR ATD ++  PGKLK VFE  G+
Sbjct: 105 LNGTVFREPIICRNIPRLVPGWTKPICIGRHAFGDQYR-ATDLIVNEPGKLKLVFEPSGS 163

BLAST of Chy2G045110 vs. TAIR 10
Match: AT1G65930.1 (cytosolic NADP+-dependent isocitrate dehydrogenase )

HSP 1 Score: 100.1 bits (248), Expect = 6.3e-22
Identity = 43/58 (74.14%), Postives = 50/58 (86.21%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVFEGQ 59
           +NG VF+EPI+ +NVP+LVPGWTKPICIGRHAFGDQYR ATD VI+ PGKL   FEG+
Sbjct: 105 LNGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYR-ATDAVIKGPGKLTMTFEGK 161

BLAST of Chy2G045110 vs. TAIR 10
Match: AT5G14590.1 (Isocitrate/isopropylmalate dehydrogenase family protein )

HSP 1 Score: 96.3 bits (238), Expect = 9.1e-21
Identity = 42/55 (76.36%), Postives = 48/55 (87.27%), Query Frame = 0

Query: 1   MNGIVFKEPILRRNVPRLVPGWTKPICIGRHAFGDQYRAATDTVIRRPGKLKFVF 56
           ++G VF+EPI+  N+PRLVPGW KPICIGRHAFGDQYR ATDTVI+ PGKLK VF
Sbjct: 175 LDGTVFREPIMCSNIPRLVPGWEKPICIGRHAFGDQYR-ATDTVIKGPGKLKMVF 228

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SLK06.2e-2273.33Peroxisomal isocitrate dehydrogenase [NADP] OS=Arabidopsis thaliana OX=3702 GN=I... [more]
Q403458.1e-2275.41Isocitrate dehydrogenase [NADP], chloroplastic (Fragment) OS=Medicago sativa OX=... [more]
P502182.3e-2175.00Isocitrate dehydrogenase [NADP] OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P502175.2e-2173.33Isocitrate dehydrogenase [NADP] OS=Solanum tuberosum OX=4113 GN=ICDH-1 PE=2 SV=1[more]
Q9SRZ68.9e-2174.14Cytosolic isocitrate dehydrogenase [NADP] OS=Arabidopsis thaliana OX=3702 GN=CIC... [more]
Match NameE-valueIdentityDescription
A0A0A0KRB41.1e-2185.00Isocitrate dehydrogenase [NADP] OS=Cucumis sativus OX=3659 GN=Csa_5G346550 PE=3 ... [more]
Q8RW691.1e-2185.00Isocitrate dehydrogenase [NADP] OS=Cucumis sativus OX=3659 GN=IDH PE=2 SV=1[more]
A0A1S4E4292.4e-2183.33Isocitrate dehydrogenase [NADP] OS=Cucumis melo OX=3656 GN=LOC103501355 PE=3 SV=... [more]
A0A6J1FJA02.4e-2183.33Isocitrate dehydrogenase [NADP] OS=Cucurbita moschata OX=3662 GN=LOC111444771 PE... [more]
A0A5A7VEU12.4e-2183.33Isocitrate dehydrogenase [NADP] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
NP_001295854.11.42e-2685.00cytosolic isocitrate dehydrogenase [Cucumis sativus] >CAD24779.1 isocitrate dehy... [more]
XP_011655148.11.42e-2685.00cytosolic isocitrate dehydrogenase isoform X2 [Cucumis sativus] >XP_031740811.1 ... [more]
XP_031740810.11.46e-2685.00cytosolic isocitrate dehydrogenase isoform X1 [Cucumis sativus][more]
KAG1367693.13.60e-2679.31putative Isocitrate dehydrogenase [NADP] [Cocos nucifera][more]
KAG6579052.13.84e-2683.33Cytosolic isocitrate dehydrogenase [NADP], partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
AT1G54340.14.4e-2373.33isocitrate dehydrogenase [more]
AT1G65930.16.3e-2274.14cytosolic NADP+-dependent isocitrate dehydrogenase [more]
AT5G14590.19.1e-2176.36Isocitrate/isopropylmalate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.718.10Isopropylmalate Dehydrogenasecoord: 1..60
e-value: 7.9E-16
score: 59.6
NoneNo IPR availablePANTHERPTHR11822:SF30PEROXISOMAL ISOCITRATE DEHYDROGENASE [NADP]coord: 1..59
NoneNo IPR availableSUPERFAMILY53659Isocitrate/Isopropylmalate dehydrogenase-likecoord: 1..56
IPR004790Isocitrate dehydrogenase NADP-dependentPANTHERPTHR11822NADP-SPECIFIC ISOCITRATE DEHYDROGENASEcoord: 1..59

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G045110.1Chy2G045110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006102 isocitrate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0043229 intracellular organelle
molecular_function GO:0004450 isocitrate dehydrogenase (NADP+) activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0051287 NAD binding